Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68820.t1 | XP_027338956.1 | 89 | 109 | 12 | 0 | 1 | 109 | 170 | 278 | 1.00E-42 | 182.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68820.t1 | Q9SY29 | 74.3 | 109 | 28 | 0 | 1 | 109 | 129 | 237 | 5.5e-37 | 154.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68820.t1 | A0A1S2XES6 | 89.9 | 109 | 11 | 0 | 1 | 109 | 153 | 261 | 7.4e-43 | 182.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052902 | MS.gene68820 | 0.826021 | 3.41E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAAAAGAATACTTTAT+TGG | - | 15232:6898-6917 | MS.gene68820:CDS | 15.0% |
| !! | AAAAGTTTATTGTTACGAAA+TGG | - | 15232:6990-7009 | MS.gene68820:CDS | 20.0% |
| ! | TTATTGTTACGAAATGGTAA+TGG | - | 15232:6996-7015 | MS.gene68820:CDS | 25.0% |
| !!! | TCTTTTTTTGTTAGTCCTTT+TGG | + | 15232:6889-6908 | MS.gene68820:intergenic | 25.0% |
| AATGGAAATGCAGCTTATAA+TGG | - | 15232:7014-7033 | MS.gene68820:CDS | 30.0% | |
| TTATAAACCTTCTCCATAAC+TGG | + | 15232:6970-6989 | MS.gene68820:intergenic | 30.0% | |
| TTATTTACCAGTTATGGAGA+AGG | - | 15232:6960-6979 | MS.gene68820:CDS | 30.0% | |
| ! | TGCACTTTATTTACCAGTTA+TGG | - | 15232:6954-6973 | MS.gene68820:CDS | 30.0% |
| !! | GATCTTTTGTTTCACTATGA+TGG | + | 15232:6788-6807 | MS.gene68820:intergenic | 30.0% |
| AGCTGTGATGATAGTAAAAG+TGG | + | 15232:6742-6761 | MS.gene68820:intergenic | 35.0% | |
| AAATAACAACCCTGCACCAA+GGG | + | 15232:6935-6954 | MS.gene68819:intergenic | 40.0% | |
| TCTAGTAGTGAGAGACCAAA+AGG | - | 15232:6871-6890 | MS.gene68820:CDS | 40.0% | |
| ! | ATTGGATTCTTTTGCACCCT+TGG | - | 15232:6916-6935 | MS.gene68820:CDS | 40.0% |
| ! | TATAATGGAGATTGCAGCGA+CGG | - | 15232:7029-7048 | MS.gene68820:CDS | 40.0% |
| CAAATAACAACCCTGCACCA+AGG | + | 15232:6936-6955 | MS.gene68820:intergenic | 45.0% | |
| !!! | GTTTTTAGCGACAGTGGGAA+TGG | - | 15232:7051-7070 | MS.gene68820:CDS | 45.0% |
| !! | TCTTTTGCACCCTTGGTGCA+GGG | - | 15232:6923-6942 | MS.gene68820:CDS | 50.0% |
| !! | TTCTTTTGCACCCTTGGTGC+AGG | - | 15232:6922-6941 | MS.gene68820:CDS | 50.0% |
| !!! | CGACGGTTTTTAGCGACAGT+GGG | - | 15232:7046-7065 | MS.gene68820:CDS | 50.0% |
| !!! | GCGACGGTTTTTAGCGACAG+TGG | - | 15232:7045-7064 | MS.gene68820:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15232 | gene | 6740 | 7074 | 6740 | ID=MS.gene68820 |
| 15232 | mRNA | 6740 | 7074 | 6740 | ID=MS.gene68820.t1;Parent=MS.gene68820 |
| 15232 | exon | 6740 | 7074 | 6740 | ID=MS.gene68820.t1.exon1;Parent=MS.gene68820.t1 |
| 15232 | CDS | 6740 | 7074 | 6740 | ID=cds.MS.gene68820.t1;Parent=MS.gene68820.t1 |
| Gene Sequence |
| Protein sequence |
