Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052902.t1 | RHN69500.1 | 87 | 69 | 6 | 3 | 22 | 89 | 1 | 67 | 2.40E-18 | 101.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052902.t1 | A0A396IVQ6 | 87.0 | 69 | 6 | 3 | 22 | 89 | 1 | 67 | 1.7e-18 | 101.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051544 | MS.gene052902 | 0.813529 | 2.44E-51 | -1.69E-46 |
| MS.gene052902 | MS.gene053356 | 0.875081 | 4.08E-68 | -1.69E-46 |
| MS.gene052902 | MS.gene058426 | 0.821233 | 4.50E-53 | -1.69E-46 |
| MS.gene052902 | MS.gene070515 | 0.806439 | 8.22E-50 | -1.69E-46 |
| MS.gene052902 | MS.gene09317 | 0.831122 | 2.00E-55 | -1.69E-46 |
| MS.gene052902 | MS.gene23193 | 0.822831 | 1.92E-53 | -1.69E-46 |
| MS.gene052902 | MS.gene25712 | 0.834506 | 2.88E-56 | -1.69E-46 |
| MS.gene052902 | MS.gene25714 | 0.834252 | 3.34E-56 | -1.69E-46 |
| MS.gene052902 | MS.gene36708 | 0.816477 | 5.42E-52 | -1.69E-46 |
| MS.gene052902 | MS.gene41476 | 0.80809 | 3.67E-50 | -1.69E-46 |
| MS.gene052902 | MS.gene53831 | 0.845545 | 3.80E-59 | -1.69E-46 |
| MS.gene052902 | MS.gene54117 | 0.800459 | 1.43E-48 | -1.69E-46 |
| MS.gene052902 | MS.gene54314 | 0.817658 | 2.94E-52 | -1.69E-46 |
| MS.gene052902 | MS.gene54316 | 0.813465 | 2.52E-51 | -1.69E-46 |
| MS.gene052902 | MS.gene56057 | 0.817336 | 3.48E-52 | -1.69E-46 |
| MS.gene052902 | MS.gene62931 | 0.814017 | 1.91E-51 | -1.69E-46 |
| MS.gene052902 | MS.gene64443 | 0.810946 | 8.95E-51 | -1.69E-46 |
| MS.gene052902 | MS.gene65036 | 0.815797 | 7.69E-52 | -1.69E-46 |
| MS.gene052902 | MS.gene65458 | 0.830142 | 3.47E-55 | -1.69E-46 |
| MS.gene052902 | MS.gene66157 | 0.804179 | 2.45E-49 | -1.69E-46 |
| MS.gene052902 | MS.gene66424 | 0.800594 | 1.34E-48 | -1.69E-46 |
| MS.gene052902 | MS.gene68819 | 0.803881 | 2.82E-49 | -1.69E-46 |
| MS.gene052902 | MS.gene68820 | 0.826021 | 3.41E-54 | -1.69E-46 |
| MS.gene052902 | MS.gene69575 | 0.812642 | 3.83E-51 | -1.69E-46 |
| MS.gene052902 | MS.gene71493 | 0.827756 | 1.31E-54 | -1.69E-46 |
| MS.gene052902 | MS.gene72477 | 0.806094 | 9.72E-50 | -1.69E-46 |
| MS.gene052902 | MS.gene73045 | 0.80768 | 4.49E-50 | -1.69E-46 |
| MS.gene052902 | MS.gene73889 | 0.829606 | 4.68E-55 | -1.69E-46 |
| MS.gene052902 | MS.gene80682 | 0.81183 | 5.75E-51 | -1.69E-46 |
| MS.gene052902 | MS.gene83829 | 0.828427 | 9.05E-55 | -1.69E-46 |
| MS.gene052902 | MS.gene84535 | 0.811797 | 5.85E-51 | -1.69E-46 |
| MS.gene052902 | MS.gene84869 | 0.803621 | 3.19E-49 | -1.69E-46 |
| MS.gene052902 | MS.gene88413 | 0.801061 | 1.08E-48 | -1.69E-46 |
| MS.gene052902 | MS.gene91136 | 0.810169 | 1.32E-50 | -1.69E-46 |
| MS.gene052902 | MS.gene98807 | 0.80698 | 6.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCGACGGTACTTCCAAAA+AGG | 0.300478 | 3.1:+71893530 | MS.gene052902:CDS |
| GTGATGAAGAGTGTTCTTAA+TGG | 0.357323 | 3.1:+71893559 | MS.gene052902:CDS |
| GATTCCAACTCCTCTTCTAA+TGG | 0.443033 | 3.1:+71893484 | MS.gene052902:CDS |
| CAAGGATGAGTGAAGGCAGC+AGG | 0.502664 | 3.1:+71893401 | MS.gene052902:CDS |
| TGCACCATTAGAAGAGGAGT+TGG | 0.504942 | 3.1:-71893488 | None:intergenic |
| CAGAAGCTTGGTTTGAGTCA+AGG | 0.516315 | 3.1:+71893383 | MS.gene052902:CDS |
| CAGTTCAATCACTACACTGT+GGG | 0.521382 | 3.1:+71893354 | MS.gene052902:CDS |
| TCTCATCAACCCACCAACAC+TGG | 0.526880 | 3.1:+71893460 | MS.gene052902:CDS |
| AACTATCTACCCATCGGTGG+TGG | 0.528156 | 3.1:-71893596 | None:intergenic |
| TTGACTTCGTCCACCACCGA+TGG | 0.548133 | 3.1:+71893586 | MS.gene052902:CDS |
| GTTGGAATCTCCAGTGTTGG+TGG | 0.553067 | 3.1:-71893470 | None:intergenic |
| TTGGAATCTCCAGTGTTGGT+GGG | 0.569606 | 3.1:-71893469 | None:intergenic |
| TAATGGTGCAAGGATGATGA+AGG | 0.569809 | 3.1:+71893501 | MS.gene052902:CDS |
| CATCCTTGCACCATTAGAAG+AGG | 0.585644 | 3.1:-71893494 | None:intergenic |
| TGACTTCGTCCACCACCGAT+GGG | 0.604322 | 3.1:+71893587 | MS.gene052902:CDS |
| GGAGTTGGAATCTCCAGTGT+TGG | 0.610509 | 3.1:-71893473 | None:intergenic |
| ACTCCTCTTCTAATGGTGCA+AGG | 0.620077 | 3.1:+71893491 | MS.gene052902:CDS |
| AGCAGGAAGAGTCAACAGTA+CGG | 0.624149 | 3.1:+71893418 | MS.gene052902:CDS |
| TTTGAGTCAAGGATGAGTGA+AGG | 0.645388 | 3.1:+71893394 | MS.gene052902:CDS |
| GTAACTAACTATCTACCCAT+CGG | 0.661836 | 3.1:-71893602 | None:intergenic |
| AGTTCAATCACTACACTGTG+GGG | 0.691304 | 3.1:+71893355 | MS.gene052902:CDS |
| GATGAAGGGTGTAAAACCGA+CGG | 0.694227 | 3.1:+71893516 | MS.gene052902:CDS |
| ACTAACTATCTACCCATCGG+TGG | 0.704765 | 3.1:-71893599 | None:intergenic |
| GTTCAATCACTACACTGTGG+GGG | 0.705459 | 3.1:+71893356 | MS.gene052902:CDS |
| ACAGTTCAATCACTACACTG+TGG | 0.712449 | 3.1:+71893353 | MS.gene052902:CDS |
| AATGGTGCAAGGATGATGAA+GGG | 0.729070 | 3.1:+71893502 | MS.gene052902:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTGATGAAGAGTGTTCTTAA+TGG | + | chr3.1:71893559-71893578 | MS.gene052902:CDS | 35.0% |
| AATGGTGCAAGGATGATGAA+GGG | + | chr3.1:71893502-71893521 | MS.gene052902:CDS | 40.0% | |
| ACAGTTCAATCACTACACTG+TGG | + | chr3.1:71893353-71893372 | MS.gene052902:CDS | 40.0% | |
| AGTTCAATCACTACACTGTG+GGG | + | chr3.1:71893355-71893374 | MS.gene052902:CDS | 40.0% | |
| CAGTTCAATCACTACACTGT+GGG | + | chr3.1:71893354-71893373 | MS.gene052902:CDS | 40.0% | |
| GATTCCAACTCCTCTTCTAA+TGG | + | chr3.1:71893484-71893503 | MS.gene052902:CDS | 40.0% | |
| TAATGGTGCAAGGATGATGA+AGG | + | chr3.1:71893501-71893520 | MS.gene052902:CDS | 40.0% | |
| TTTGAGTCAAGGATGAGTGA+AGG | + | chr3.1:71893394-71893413 | MS.gene052902:CDS | 40.0% | |
| AACCGACGGTACTTCCAAAA+AGG | + | chr3.1:71893530-71893549 | MS.gene052902:CDS | 45.0% | |
| ACCGACGGTACTTCCAAAAA+GGG | + | chr3.1:71893531-71893550 | MS.gene052902:CDS | 45.0% | |
| ACTCCTCTTCTAATGGTGCA+AGG | + | chr3.1:71893491-71893510 | MS.gene052902:CDS | 45.0% | |
| AGCAGGAAGAGTCAACAGTA+CGG | + | chr3.1:71893418-71893437 | MS.gene052902:CDS | 45.0% | |
| CATCCTTGCACCATTAGAAG+AGG | - | chr3.1:71893497-71893516 | None:intergenic | 45.0% | |
| GTTCAATCACTACACTGTGG+GGG | + | chr3.1:71893356-71893375 | MS.gene052902:CDS | 45.0% | |
| TGCACCATTAGAAGAGGAGT+TGG | - | chr3.1:71893491-71893510 | None:intergenic | 45.0% | |
| ! | CAGAAGCTTGGTTTGAGTCA+AGG | + | chr3.1:71893383-71893402 | MS.gene052902:CDS | 45.0% |
| ! | GATGAAGGGTGTAAAACCGA+CGG | + | chr3.1:71893516-71893535 | MS.gene052902:CDS | 45.0% |
| ! | TTCATCACAGCTCCCCTTTT+TGG | - | chr3.1:71893547-71893566 | None:intergenic | 45.0% |
| !! | TTGGAATCTCCAGTGTTGGT+GGG | - | chr3.1:71893472-71893491 | None:intergenic | 45.0% |
| CCGACGGTACTTCCAAAAAG+GGG | + | chr3.1:71893532-71893551 | MS.gene052902:CDS | 50.0% | |
| GGAGTTGGAATCTCCAGTGT+TGG | - | chr3.1:71893476-71893495 | None:intergenic | 50.0% | |
| TCTCATCAACCCACCAACAC+TGG | + | chr3.1:71893460-71893479 | MS.gene052902:CDS | 50.0% | |
| ! | GTTGGAATCTCCAGTGTTGG+TGG | - | chr3.1:71893473-71893492 | None:intergenic | 50.0% |
| !!! | CCCCTTTTTGGAAGTACCGT+CGG | - | chr3.1:71893535-71893554 | None:intergenic | 50.0% |
| CAAGGATGAGTGAAGGCAGC+AGG | + | chr3.1:71893401-71893420 | MS.gene052902:CDS | 55.0% | |
| TGACTTCGTCCACCACCGAT+GGG | + | chr3.1:71893587-71893606 | MS.gene052902:CDS | 55.0% | |
| TTGACTTCGTCCACCACCGA+TGG | + | chr3.1:71893586-71893605 | MS.gene052902:CDS | 55.0% | |
| !! | TGTGGGGGTGCTCAGAAGCT+TGG | + | chr3.1:71893371-71893390 | MS.gene052902:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 71893343 | 71893612 | 71893343 | ID=MS.gene052902 |
| chr3.1 | mRNA | 71893343 | 71893612 | 71893343 | ID=MS.gene052902.t1;Parent=MS.gene052902 |
| chr3.1 | exon | 71893343 | 71893612 | 71893343 | ID=MS.gene052902.t1.exon1;Parent=MS.gene052902.t1 |
| chr3.1 | CDS | 71893343 | 71893612 | 71893343 | ID=cds.MS.gene052902.t1;Parent=MS.gene052902.t1 |
| Gene Sequence |
| Protein sequence |
