Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68894.t1 | TKY72580.1 | 55.4 | 92 | 20 | 1 | 1 | 71 | 1 | 92 | 3.70E-16 | 94.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68894.t1 | Q9FH22 | 85.0 | 40 | 6 | 0 | 32 | 71 | 68 | 107 | 2.3e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68894.t1 | A0A0S3RI40 | 54.5 | 88 | 23 | 1 | 1 | 71 | 1 | 88 | 2.9e-15 | 90.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene68894 | 0.837656 | 4.57E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68894.t1 | MTR_2g436020 | 74.306 | 144 | 5 | 1 | 1 | 112 | 1 | 144 | 1.50e-59 | 179 |
| MS.gene68894.t1 | MTR_2g436250 | 74.306 | 144 | 5 | 1 | 1 | 112 | 1 | 144 | 1.50e-59 | 179 |
| MS.gene68894.t1 | MTR_4g120160 | 76.471 | 51 | 12 | 0 | 30 | 80 | 60 | 110 | 2.76e-24 | 89.4 |
| MS.gene68894.t1 | MTR_3g073960 | 62.791 | 43 | 16 | 0 | 32 | 74 | 90 | 132 | 7.02e-16 | 69.3 |
| MS.gene68894.t1 | MTR_8g022950 | 38.393 | 112 | 58 | 4 | 2 | 103 | 3 | 113 | 9.25e-15 | 65.5 |
| MS.gene68894.t1 | MTR_3g087700 | 43.836 | 73 | 40 | 1 | 1 | 72 | 92 | 164 | 3.61e-13 | 62.8 |
| MS.gene68894.t1 | MTR_3g108290 | 44.737 | 76 | 40 | 2 | 9 | 82 | 330 | 405 | 5.96e-13 | 63.9 |
| MS.gene68894.t1 | MTR_5g013770 | 52.830 | 53 | 24 | 1 | 26 | 78 | 79 | 130 | 2.46e-12 | 60.1 |
| MS.gene68894.t1 | MTR_8g008840 | 58.537 | 41 | 17 | 0 | 32 | 72 | 38 | 78 | 1.27e-11 | 57.0 |
| MS.gene68894.t1 | MTR_4g099050 | 50.943 | 53 | 26 | 0 | 26 | 78 | 67 | 119 | 1.78e-11 | 57.8 |
| MS.gene68894.t1 | MTR_4g124930 | 47.143 | 70 | 30 | 2 | 25 | 94 | 171 | 233 | 1.85e-11 | 58.9 |
| MS.gene68894.t1 | MTR_4g124930 | 54.000 | 50 | 22 | 1 | 25 | 74 | 171 | 219 | 2.29e-11 | 58.9 |
| MS.gene68894.t1 | MTR_2g042970 | 58.333 | 48 | 19 | 1 | 25 | 72 | 179 | 225 | 2.60e-11 | 58.5 |
| MS.gene68894.t1 | MTR_1g010140 | 48.387 | 62 | 30 | 2 | 23 | 82 | 265 | 326 | 9.15e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68894.t1 | AT5G49120 | 56.180 | 89 | 33 | 2 | 28 | 112 | 64 | 150 | 1.02e-28 | 102 |
| MS.gene68894.t1 | AT1G22160 | 63.415 | 41 | 15 | 0 | 32 | 72 | 78 | 118 | 2.76e-14 | 64.7 |
| MS.gene68894.t1 | AT1G78020 | 42.623 | 61 | 35 | 0 | 32 | 92 | 89 | 149 | 3.57e-14 | 65.1 |
| MS.gene68894.t1 | AT4G39795 | 50.000 | 52 | 26 | 0 | 26 | 77 | 67 | 118 | 1.24e-13 | 62.8 |
| MS.gene68894.t1 | AT5G47060 | 60.000 | 45 | 18 | 0 | 28 | 72 | 93 | 137 | 1.14e-12 | 61.2 |
| MS.gene68894.t1 | AT5G47060 | 60.000 | 45 | 18 | 0 | 28 | 72 | 93 | 137 | 1.14e-12 | 61.2 |
| MS.gene68894.t1 | AT3G22550 | 41.892 | 74 | 33 | 2 | 7 | 73 | 193 | 263 | 9.93e-12 | 60.1 |
| MS.gene68894.t1 | AT1G74940 | 43.902 | 82 | 38 | 3 | 30 | 111 | 148 | 221 | 1.31e-11 | 59.3 |
| MS.gene68894.t1 | AT4G17670 | 56.818 | 44 | 19 | 0 | 29 | 72 | 73 | 116 | 1.91e-11 | 57.8 |
| MS.gene68894.t1 | AT2G44670 | 57.778 | 45 | 19 | 0 | 28 | 72 | 12 | 56 | 2.47e-11 | 55.8 |
| MS.gene68894.t1 | AT5G65040 | 54.545 | 44 | 20 | 0 | 32 | 75 | 57 | 100 | 3.17e-11 | 56.2 |
| MS.gene68894.t1 | AT5G20700 | 53.061 | 49 | 22 | 1 | 24 | 72 | 174 | 221 | 7.06e-11 | 57.4 |
Find 10 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCTTATCATCATGAAAA+AGG | 0.300416 | 2.4:-55115669 | MS.gene68894:CDS |
| AATGAGCAAAACCACCGGTT+TGG | 0.436942 | 2.4:+55115637 | None:intergenic |
| GTAGTGTTAGAATCTCAAAA+AGG | 0.513194 | 2.4:-55116216 | MS.gene68894:CDS |
| TATTAACAATAGCAAGAAGA+AGG | 0.566617 | 2.4:-55116187 | MS.gene68894:CDS |
| AAAGGAAGAAGAAACCAAAC+CGG | 0.585292 | 2.4:-55115651 | MS.gene68894:CDS |
| GGATAGAGCTTTCTGTAGTG+TGG | 0.592385 | 2.4:-55115790 | MS.gene68894:CDS |
| TAGATGCAAGCAAATTGTGA+TGG | 0.624999 | 2.4:-55115763 | MS.gene68894:CDS |
| GGAAGAAGAAACCAAACCGG+TGG | 0.683708 | 2.4:-55115648 | MS.gene68894:CDS |
| GTATCAATGAGCAAAACCAC+CGG | 0.685317 | 2.4:+55115632 | None:intergenic |
| AAGATGAAGAAATTGCAGCA+TGG | 0.688850 | 2.4:+55115700 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATAAAAACAAAGATCT+AGG | + | chr2.4:55115976-55115995 | None:intergenic | 15.0% |
| !! | AATATAAAGTTTCATAACAT+AGG | + | chr2.4:55115915-55115934 | None:intergenic | 15.0% |
| !!! | ATTGTTTTGTTAATCACTTT+TGG | + | chr2.4:55115710-55115729 | None:intergenic | 20.0% |
| !!! | TGTTTATTTTTTTGTGAAGA+GGG | - | chr2.4:55116054-55116073 | MS.gene68894:CDS | 20.0% |
| ! | TATTAACAATAGCAAGAAGA+AGG | - | chr2.4:55115679-55115698 | MS.gene68894:CDS | 25.0% |
| !!! | GTGTTTATTTTTTTGTGAAG+AGG | - | chr2.4:55116053-55116072 | MS.gene68894:CDS | 25.0% |
| !!! | GTTTATTTTTTTGTGAAGAG+GGG | - | chr2.4:55116055-55116074 | MS.gene68894:CDS | 25.0% |
| GCTTCTTATCATCATGAAAA+AGG | - | chr2.4:55116197-55116216 | MS.gene68894:CDS | 30.0% | |
| GTAGTGTTAGAATCTCAAAA+AGG | - | chr2.4:55115650-55115669 | MS.gene68894:CDS | 30.0% | |
| ! | ATGATGGAAATAAGAACTCA+AGG | + | chr2.4:55115774-55115793 | None:intergenic | 30.0% |
| ! | TACATATAGATGTCTTTTCC+AGG | + | chr2.4:55115866-55115885 | None:intergenic | 30.0% |
| AAAGGAAGAAGAAACCAAAC+CGG | - | chr2.4:55116215-55116234 | MS.gene68894:CDS | 35.0% | |
| AAGATGAAGAAATTGCAGCA+TGG | + | chr2.4:55116169-55116188 | None:intergenic | 35.0% | |
| CCTGAAAATGAGATTCATGA+TGG | + | chr2.4:55115790-55115809 | None:intergenic | 35.0% | |
| GTGTTAGAATCTCAAAAAGG+TGG | - | chr2.4:55115653-55115672 | MS.gene68894:CDS | 35.0% | |
| TAGATGCAAGCAAATTGTGA+TGG | - | chr2.4:55116103-55116122 | MS.gene68894:intron | 35.0% | |
| TGTAGGAAAAAGTTGTTACC+TGG | - | chr2.4:55115845-55115864 | MS.gene68894:intron | 35.0% | |
| TTAGAATCTCAAAAAGGTGG+TGG | - | chr2.4:55115656-55115675 | MS.gene68894:CDS | 35.0% | |
| ! | CCATCATGAATCTCATTTTC+AGG | - | chr2.4:55115787-55115806 | MS.gene68894:CDS | 35.0% |
| !!! | TTGAGCTTTGTTTTCTCTGT+AGG | - | chr2.4:55115828-55115847 | MS.gene68894:intron | 35.0% |
| GGATAGAGCTTTCTGTAGTG+TGG | - | chr2.4:55116076-55116095 | MS.gene68894:intron | 45.0% | |
| ! | AATGAGCAAAACCACCGGTT+TGG | + | chr2.4:55116232-55116251 | None:intergenic | 45.0% |
| GGAAGAAGAAACCAAACCGG+TGG | - | chr2.4:55116218-55116237 | MS.gene68894:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 55115635 | 55116253 | 55115635 | ID=MS.gene68894 |
| chr2.4 | mRNA | 55115635 | 55116253 | 55115635 | ID=MS.gene68894.t1;Parent=MS.gene68894 |
| chr2.4 | exon | 55116176 | 55116253 | 55116176 | ID=MS.gene68894.t1.exon1;Parent=MS.gene68894.t1 |
| chr2.4 | CDS | 55116176 | 55116253 | 55116176 | ID=cds.MS.gene68894.t1;Parent=MS.gene68894.t1 |
| chr2.4 | exon | 55116000 | 55116079 | 55116000 | ID=MS.gene68894.t1.exon2;Parent=MS.gene68894.t1 |
| chr2.4 | CDS | 55116000 | 55116079 | 55116000 | ID=cds.MS.gene68894.t1;Parent=MS.gene68894.t1 |
| chr2.4 | exon | 55115635 | 55115815 | 55115635 | ID=MS.gene68894.t1.exon3;Parent=MS.gene68894.t1 |
| chr2.4 | CDS | 55115635 | 55115815 | 55115635 | ID=cds.MS.gene68894.t1;Parent=MS.gene68894.t1 |
| Gene Sequence |
| Protein sequence |
