Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68988.t1 | XP_013453119.1 | 96.7 | 122 | 4 | 0 | 1 | 122 | 100 | 221 | 1.80E-64 | 255 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68988.t1 | Q9ZSY8 | 72.3 | 130 | 27 | 3 | 2 | 122 | 143 | 272 | 8.4e-47 | 187.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68988.t1 | A0A072UBZ5 | 96.7 | 122 | 4 | 0 | 1 | 122 | 100 | 221 | 1.3e-64 | 255.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059607 | MS.gene68988 | 0.895582 | 8.36E-76 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAACGTACATAACTAAA+GGG | + | 41657:17959-17978 | MS.gene68988:intron | 20.0% |
| !! | TTTAAAGGAGTGATATTAAT+AGG | + | 41657:18032-18051 | MS.gene68988:intron | 20.0% |
| !!! | GTTATGTACGTTTTAAATAA+AGG | - | 41657:17955-17974 | MS.gene68988:intergenic | 20.0% |
| !!! | TTTAAAACGTACATAACTAA+AGG | + | 41657:17958-17977 | MS.gene68988:intron | 20.0% |
| !! | AACAACTAAACATCTTTTCT+AGG | - | 41657:17663-17682 | MS.gene68988:intergenic | 25.0% |
| !! | ACAACTAAACATCTTTTCTA+GGG | - | 41657:17662-17681 | MS.gene68988:intergenic | 25.0% |
| !! | TAGGTTTAAGATAACAATCT+CGG | + | 41657:17743-17762 | MS.gene68988:intron | 25.0% |
| !!! | AAAGTGGATCTCAAATTTTT+TGG | + | 41657:17613-17632 | MS.gene68988:CDS | 25.0% |
| !!! | TAGTACCGATTTTAGATTTA+AGG | + | 41657:17833-17852 | MS.gene68988:intron | 25.0% |
| !!! | TTTTGATGAATGAAAAGTGT+AGG | + | 41657:18109-18128 | MS.gene68988:intron | 25.0% |
| ACAACACTGATACAATGAAA+AGG | - | 41657:17909-17928 | MS.gene68987:intergenic | 30.0% | |
| ATCACCATCTTTATCTTCAT+AGG | - | 41657:18224-18243 | MS.gene68988:intergenic | 30.0% | |
| ATGAAGATAAAGATGGTGAT+TGG | + | 41657:18224-18243 | MS.gene68988:CDS | 30.0% | |
| ATTTGAAAACATCTCGATGA+CGG | - | 41657:17881-17900 | MS.gene68988:intergenic | 30.0% | |
| CCTTTATGTAAAAGTTAGCA+TGG | + | 41657:17570-17589 | MS.gene68988:CDS | 30.0% | |
| GTTGATATTGTGGAGAATTA+CGG | + | 41657:17779-17798 | MS.gene68988:intron | 30.0% | |
| TAAAAATCTCCTTAGCTCAA+TGG | - | 41657:18016-18035 | MS.gene68988:intergenic | 30.0% | |
| TATGTAAAAGTTAGCATGGA+TGG | + | 41657:17574-17593 | MS.gene68988:CDS | 30.0% | |
| TTCTTTCAGGAAGAATTCAA+TGG | + | 41657:17495-17514 | MS.gene68988:CDS | 30.0% | |
| TTGAATTCTTCCTGAAAGAA+CGG | - | 41657:17495-17514 | MS.gene68988:intergenic | 30.0% | |
| TTTGTGTGAAAGTAGATTGA+TGG | + | 41657:18168-18187 | MS.gene68988:CDS | 30.0% | |
| ! | ACACGCCTTAAATCTAAAAT+CGG | - | 41657:17841-17860 | MS.gene68988:intergenic | 30.0% |
| ! | AGGTTTAAGATAACAATCTC+GGG | + | 41657:17744-17763 | MS.gene68988:intron | 30.0% |
| ! | ATGAAAAGTGTAGGTCAATT+TGG | + | 41657:18118-18137 | MS.gene68988:intron | 30.0% |
| ! | CCATGCTAACTTTTACATAA+AGG | - | 41657:17573-17592 | MS.gene68988:intergenic | 30.0% |
| ! | TGAGCTAAGGAGATTTTTAA+AGG | + | 41657:18017-18036 | MS.gene68988:intron | 30.0% |
| !! | TACATAAAGGCATCCAAATT+TGG | - | 41657:17560-17579 | MS.gene68988:intergenic | 30.0% |
| !!! | ATCATTCTTTTGAGATTGAG+TGG | - | 41657:17521-17540 | MS.gene68988:intergenic | 30.0% |
| !!! | CAAATTTTTTGGCACTTACA+AGG | + | 41657:17624-17643 | MS.gene68988:CDS | 30.0% |
| CATCTTTATCTTCATAGGTG+AGG | - | 41657:18219-18238 | MS.gene68988:intergenic | 35.0% | |
| CTCACCTATGAAGATAAAGA+TGG | + | 41657:18217-18236 | MS.gene68988:CDS | 35.0% | |
| GATGATGTAGAAGCCAAATT+TGG | + | 41657:17544-17563 | MS.gene68988:CDS | 35.0% | |
| GTACATAACTAAAGGGTAGT+TGG | + | 41657:17966-17985 | MS.gene68988:intron | 35.0% | |
| ! | GTAGTTGGTTCATATGCTAA+GGG | + | 41657:17981-18000 | MS.gene68988:intron | 35.0% |
| AGATCCACTTTCCTCAAGTA+TGG | - | 41657:17604-17623 | MS.gene68987:intergenic | 40.0% | |
| ATCCACTTTCCTCAAGTATG+GGG | - | 41657:17602-17621 | MS.gene68988:intergenic | 40.0% | |
| CGTCATGATCGTTGATATTG+TGG | + | 41657:17769-17788 | MS.gene68988:intron | 40.0% | |
| GATCCACTTTCCTCAAGTAT+GGG | - | 41657:17603-17622 | MS.gene68988:intergenic | 40.0% | |
| GGACATATTCTGAACCATGA+AGG | - | 41657:18198-18217 | MS.gene68988:intergenic | 40.0% | |
| TGGAGAATTACGGTAAAACG+CGG | + | 41657:17789-17808 | MS.gene68988:intron | 40.0% | |
| TTCTTCCTGAAAGAACGGAT+TGG | - | 41657:17490-17509 | MS.gene68988:intergenic | 40.0% | |
| ! | AGATTGATGGATCTCCTTCA+TGG | + | 41657:18181-18200 | MS.gene68988:CDS | 40.0% |
| ! | GGTAGTTGGTTCATATGCTA+AGG | + | 41657:17980-17999 | MS.gene68988:intron | 40.0% |
| ! | TCTCATTTTGCTTGTGCACT+AGG | + | 41657:17724-17743 | MS.gene68988:intron | 40.0% |
| ! | TGGTTGATTCCATTGAGCTA+AGG | + | 41657:18004-18023 | MS.gene68988:intron | 40.0% |
| !!! | TCTCATGGAGTTTTTGGTCA+AGG | + | 41657:18142-18161 | MS.gene68988:CDS | 40.0% |
| !!! | TTTGGCTCTCATGGAGTTTT+TGG | + | 41657:18136-18155 | MS.gene68988:CDS | 40.0% |
| AGTGTTGTGTCCGATGTTTG+TGG | + | 41657:17921-17940 | MS.gene68988:intron | 45.0% | |
| GATGGTGATTGGATGCTAGT+TGG | + | 41657:18235-18254 | MS.gene68988:CDS | 45.0% | |
| ! | GTTGGTTCATATGCTAAGGG+TGG | + | 41657:17984-18003 | MS.gene68988:intron | 45.0% |
| !! | AGTTGGTGATGTTCCATGGA+AGG | + | 41657:18252-18271 | MS.gene68988:CDS | 45.0% |
| !! | GTAGGTCAATTTGGCTCTCA+TGG | + | 41657:18127-18146 | MS.gene68988:intron | 45.0% |
| !! | TGCTAGTTGGTGATGTTCCA+TGG | + | 41657:18248-18267 | MS.gene68988:CDS | 45.0% |
| !! | TTCCTGAAAGAACGGATTGG+TGG | - | 41657:17487-17506 | MS.gene68988:intergenic | 45.0% |
| GGCCTAACAGCCACAAACAT+CGG | - | 41657:17934-17953 | MS.gene68988:intergenic | 50.0% | |
| GTCCGATGTTTGTGGCTGTT+AGG | + | 41657:17929-17948 | MS.gene68988:intron | 50.0% | |
| TGCCCCATACTTGAGGAAAG+TGG | + | 41657:17597-17616 | MS.gene68988:CDS | 50.0% | |
| GGCCACCAATCCGTTCTTTC+AGG | + | 41657:17482-17501 | MS.gene68988:CDS | 55.0% | |
| TGGATGGTGCCCCATACTTG+AGG | + | 41657:17590-17609 | MS.gene68988:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 41657 | gene | 17465 | 18272 | 17465 | ID=MS.gene68988 |
| 41657 | mRNA | 17465 | 18272 | 17465 | ID=MS.gene68988.t1;Parent=MS.gene68988 |
| 41657 | exon | 17465 | 17691 | 17465 | ID=MS.gene68988.t1.exon1;Parent=MS.gene68988.t1 |
| 41657 | CDS | 17465 | 17691 | 17465 | ID=cds.MS.gene68988.t1;Parent=MS.gene68988.t1 |
| 41657 | exon | 18131 | 18272 | 18131 | ID=MS.gene68988.t1.exon2;Parent=MS.gene68988.t1 |
| 41657 | CDS | 18131 | 18272 | 18131 | ID=cds.MS.gene68988.t1;Parent=MS.gene68988.t1 |
| Gene Sequence |
| Protein sequence |
