AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69067

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69067.t1 MTR_7g081150 42.063 126 64 2 225 350 206 322 8.37e-20 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 97 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCAACCATTGTCTTTCT+TGG 0.215242 7.4:-25753157 MS.gene69067:CDS
ATTAGTCGGTGGACTTTCTA+TGG 0.229945 7.4:-25752882 MS.gene69067:CDS
CGTGGAACCTTTCGAGTATC+TGG 0.232195 7.4:-25753525 MS.gene69067:CDS
CATTTACAATCCATCTGTTT+AGG 0.232337 7.4:-25754381 MS.gene69067:CDS
AGTGATTTCCAGTGTGGTTT+TGG 0.260198 7.4:-25753415 MS.gene69067:CDS
TTCCAGGGAATGCTGTAAAA+TGG 0.261175 7.4:+25753377 None:intergenic
GACAATCGAATAAGCATTAC+AGG 0.311358 7.4:-25753325 MS.gene69067:CDS
TCTATGGGGTGGTATTGTTT+TGG 0.319063 7.4:-25752866 MS.gene69067:CDS
ATTTACAATCCATCTGTTTA+GGG 0.327573 7.4:-25754380 MS.gene69067:CDS
AAAGATTAGCTCAATATATA+AGG 0.338961 7.4:-25753636 MS.gene69067:CDS
GGGGAGGATGTGTTTACATT+TGG 0.350400 7.4:-25754628 MS.gene69067:CDS
TTTATTCATATCACTTCATT+TGG 0.350430 7.4:+25752830 None:intergenic
TTGGTATTTCCTACAACATA+TGG 0.355189 7.4:-25753138 MS.gene69067:CDS
AGAAAACTGAAATACAGTTT+TGG 0.363010 7.4:-25753597 MS.gene69067:CDS
GGGATTGGAAGCCTACTTCC+AGG 0.368435 7.4:+25753361 None:intergenic
TCTGTATGGTCGTCCTTGCT+TGG 0.371823 7.4:-25753055 MS.gene69067:CDS
TAATCTCAGGCTTCCTTTGA+AGG 0.377343 7.4:+25754587 None:intergenic
CTGGTTGTTCCATATGTTGT+AGG 0.398848 7.4:+25753129 None:intergenic
ATTCATATCACTTCATTTGG+TGG 0.403378 7.4:+25752833 None:intergenic
TGTTTGTGCCGTTGCTGTAA+TGG 0.403814 7.4:-25753011 MS.gene69067:CDS
CGGCGGATAATCTTCATTGT+AGG 0.415373 7.4:+25754540 None:intergenic
TGATTGAAGATGCATCAAAA+TGG 0.426211 7.4:+25752934 None:intergenic
TCGTCCTTGCTTGGGTTGTT+CGG 0.428602 7.4:-25753046 MS.gene69067:CDS
TCTTTCATTGCCCAAGGGAT+TGG 0.428984 7.4:+25753346 None:intergenic
CTGTATGGTCGTCCTTGCTT+GGG 0.431533 7.4:-25753054 MS.gene69067:CDS
CGTATTCGCCCTCGGAAAAC+CGG 0.443085 7.4:-25754354 MS.gene69067:CDS
GGACATGGATAATTGATAAC+TGG 0.448053 7.4:+25753110 None:intergenic
GATGCTGAAATACTTGCAAC+CGG 0.452382 7.4:-25754649 MS.gene69067:CDS
GCGAGTTTGATGAGAAGAAT+GGG 0.460849 7.4:-25753675 MS.gene69067:CDS
GTTGCTGTAATGGTTCTTGT+TGG 0.464949 7.4:-25753001 MS.gene69067:CDS
AATTTCAAAGCAACACCTAC+AGG 0.465289 7.4:+25754685 None:intergenic
TTAGTCGGTGGACTTTCTAT+GGG 0.465952 7.4:-25752881 MS.gene69067:CDS
GAAGTAGGCTTCCAATCCCT+TGG 0.467360 7.4:-25753357 MS.gene69067:CDS
AAAGGAAGCCTGAGATTACA+AGG 0.475383 7.4:-25754582 MS.gene69067:CDS
CCATTCTGTTCTTTGTGAAG+TGG 0.480652 7.4:+25753085 None:intergenic
TTGTAGGGAAAACTGTCATC+AGG 0.481762 7.4:+25754556 None:intergenic
GCAGCTCATTGTCATCCAGC+TGG 0.484808 7.4:+25753290 None:intergenic
GAAAACCGGTTGGACATTCA+AGG 0.487357 7.4:-25754340 MS.gene69067:intron
AGGAAACATGTTCACCCAGC+TGG 0.490868 7.4:-25753305 MS.gene69067:CDS
TTACAGCATTCCCTGGAAGT+AGG 0.494539 7.4:-25753372 MS.gene69067:CDS
GGCGAGTTTGATGAGAAGAA+TGG 0.497291 7.4:-25753676 MS.gene69067:CDS
GAAGTGTTTGTTACATTAGT+CGG 0.497356 7.4:-25752896 MS.gene69067:CDS
TCAGGAAACCTTGTAATCTC+AGG 0.510173 7.4:+25754574 None:intergenic
CGATTGTCTTTCATTGCCCA+AGG 0.511834 7.4:+25753340 None:intergenic
GGTATAGTCATGGTCAAACT+CGG 0.513220 7.4:+25753237 None:intergenic
CAAACTCGCCACGTAGGAGG+TGG 0.513706 7.4:+25753689 None:intergenic
GGCGGATAATCTTCATTGTA+GGG 0.514262 7.4:+25754541 None:intergenic
AGAATGGCCGCCTTTCTGTA+TGG 0.519744 7.4:-25753069 MS.gene69067:CDS
TTCTGTTCTTTGTGAAGTGG+CGG 0.520068 7.4:+25753088 None:intergenic
GCTTTCTGTATACTAGTGAA+TGG 0.523901 7.4:+25752974 None:intergenic
ACTCACCTTGAATGTCCAAC+CGG 0.528662 7.4:+25754335 None:intergenic
CTGTTTCACGTATCCTTCAA+AGG 0.534026 7.4:-25754600 MS.gene69067:CDS
TGGATGACAATGAGCTGCAT+TGG 0.535155 7.4:-25753285 MS.gene69067:CDS
CTTTGTGAAGTGGCGGGACA+TGG 0.535677 7.4:+25753095 None:intergenic
AATGCTGTAAAATGGTTTCG+TGG 0.540864 7.4:+25753385 None:intergenic
TCTGTTCTTTGTGAAGTGGC+GGG 0.543395 7.4:+25753089 None:intergenic
AAGTAGGCTTCCAATCCCTT+GGG 0.548575 7.4:-25753356 MS.gene69067:CDS
GTATAGTCATGGTCAAACTC+GGG 0.560488 7.4:+25753238 None:intergenic
TAGGGCCACGTATTCGCCCT+CGG 0.561257 7.4:-25754362 MS.gene69067:CDS
CAGATACTCGAAAGGTTCCA+CGG 0.563159 7.4:+25753526 None:intergenic
GTGGAACCTTTCGAGTATCT+GGG 0.569477 7.4:-25753524 MS.gene69067:intron
AATACGTGGCCCTAAACAGA+TGG 0.571150 7.4:+25754371 None:intergenic
TCGGTGGACTTTCTATGGGG+TGG 0.576456 7.4:-25752877 MS.gene69067:CDS
ATGCTGTAAAATGGTTTCGT+GGG 0.580160 7.4:+25753386 None:intergenic
CAGCTCATTGTCATCCAGCT+GGG 0.585157 7.4:+25753291 None:intergenic
GGCACTTTCCAAAACCACAC+TGG 0.587089 7.4:+25753407 None:intergenic
AAATGTAAACACATCCTCCC+CGG 0.588798 7.4:+25754630 None:intergenic
TTCGCCCTCGGAAAACCGGT+TGG 0.588799 7.4:-25754350 MS.gene69067:CDS
CATCAAACTCGCCACGTAGG+AGG 0.596975 7.4:+25753686 None:intergenic
TTACAATCAGCACATGCGTA+AGG 0.601176 7.4:-25753209 MS.gene69067:CDS
CTGCAGAGTGATTTCCAGTG+TGG 0.609136 7.4:-25753421 MS.gene69067:intron
AACAAGAACCATTACAGCAA+CGG 0.610228 7.4:+25753003 None:intergenic
ATGCTGAAATACTTGCAACC+GGG 0.610321 7.4:-25754648 MS.gene69067:CDS
GGAAATACCAAGAAAGACAA+TGG 0.611367 7.4:+25753150 None:intergenic
CCACTTCACAAAGAACAGAA+TGG 0.620190 7.4:-25753085 MS.gene69067:CDS
GTGTTTGTTACATTAGTCGG+TGG 0.622983 7.4:-25752893 MS.gene69067:CDS
CATGTGCTGATTGTAATCGT+AGG 0.627777 7.4:+25753216 None:intergenic
CTACAATGAAGATTATCCGC+CGG 0.630707 7.4:-25754539 MS.gene69067:intron
GATTGTCTTTCATTGCCCAA+GGG 0.631392 7.4:+25753341 None:intergenic
GCAAGGACGACCATACAGAA+AGG 0.632075 7.4:+25753059 None:intergenic
GGATTGGAAGCCTACTTCCA+GGG 0.634042 7.4:+25753362 None:intergenic
TGTAATCGTAGGTATAGTCA+TGG 0.636595 7.4:+25753227 None:intergenic
TGCTGAAATACTTGCAACCG+GGG 0.637362 7.4:-25754647 MS.gene69067:CDS
ATTTCAAAGCAACACCTACA+GGG 0.647287 7.4:+25754686 None:intergenic
AAAACCGAACAACCCAAGCA+AGG 0.649732 7.4:+25753042 None:intergenic
AACTTACCCAGATACTCGAA+AGG 0.655831 7.4:+25753518 None:intergenic
CGAGTTTGATGAGAAGAATG+GGG 0.665142 7.4:-25753674 MS.gene69067:CDS
CGTAGGAGGTGGGTTAAACG+AGG 0.665859 7.4:+25753700 None:intergenic
AAACTCGCCACGTAGGAGGT+GGG 0.667000 7.4:+25753690 None:intergenic
TAGTCGGTGGACTTTCTATG+GGG 0.669112 7.4:-25752880 MS.gene69067:CDS
ACTTCAGACTTCATCCCTGT+AGG 0.673784 7.4:-25754700 MS.gene69067:CDS
CGTTTAACCCACCTCCTACG+TGG 0.674719 7.4:-25753697 MS.gene69067:CDS
TGAAATACTTGCAACCGGGG+AGG 0.691742 7.4:-25754644 MS.gene69067:CDS
TATAGTCATGGTCAAACTCG+GGG 0.696239 7.4:+25753239 None:intergenic
AGGACGACCATACAGAAAGG+CGG 0.698524 7.4:+25753062 None:intergenic
AAAATGTTAGGAGTCGTCCG+TGG 0.700444 7.4:-25753543 MS.gene69067:CDS
TCTCATCAAACTCGCCACGT+AGG 0.711118 7.4:+25753683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATATTCAAATAATTGAT+AGG - chr7.4:25753816-25753835 MS.gene69067:intron 10.0%
!!! AAAACATTGAAAACTATTTT+AGG + chr7.4:25753343-25753362 None:intergenic 15.0%
!!! TAAAATAGTTTTCAATGTTT+TGG - chr7.4:25753342-25753361 MS.gene69067:CDS 15.0%
!! AAAAATAGAGGACAAAAATT+AGG + chr7.4:25753119-25753138 None:intergenic 20.0%
!! ATTACTTGTACCTATATAAT+TGG + chr7.4:25753589-25753608 None:intergenic 20.0%
!!! AAAGATTAGCTCAATATATA+AGG - chr7.4:25753911-25753930 MS.gene69067:intron 20.0%
!!! TTTCCTTTTTCTTTTTAGTA+AGG + chr7.4:25753625-25753644 None:intergenic 20.0%
!!! TTTTTTGATTACCATGTTAT+AGG - chr7.4:25753135-25753154 MS.gene69067:CDS 20.0%
! AAGAAAAAGGAAAACAGAAA+TGG - chr7.4:25753632-25753651 MS.gene69067:CDS 25.0%
! ATTAAGCTTAGTTAGATGTA+GGG + chr7.4:25753312-25753331 None:intergenic 25.0%
! ATTTACAATCCATCTGTTTA+GGG - chr7.4:25753167-25753186 MS.gene69067:CDS 25.0%
! CATGGTAATCAAAAAAATAG+AGG + chr7.4:25753131-25753150 None:intergenic 25.0%
! GTAAAAACATTATCAAGCAT+CGG + chr7.4:25753719-25753738 None:intergenic 25.0%
! TAGAAGAAAAACAGTTAGAA+TGG + chr7.4:25753053-25753072 None:intergenic 25.0%
! TATCTTGCTACAAAAATGTT+AGG - chr7.4:25753992-25754011 MS.gene69067:intron 25.0%
! TCACAATGAATCACAATATT+TGG + chr7.4:25753511-25753530 None:intergenic 25.0%
! TTCATTGTACTACTATTAGT+TGG + chr7.4:25753558-25753577 None:intergenic 25.0%
!! AGAAAACTGAAATACAGTTT+TGG - chr7.4:25753950-25753969 MS.gene69067:intron 25.0%
AACAGTATATCAAATCAAGC+AGG + chr7.4:25753390-25753409 None:intergenic 30.0%
ACAAAACCAACAAATGGTTT+GGG + chr7.4:25753288-25753307 None:intergenic 30.0%
ACAGAGCAAATTAAGGATAA+TGG + chr7.4:25753079-25753098 None:intergenic 30.0%
ACTTGTACCTATATAATTGG+TGG + chr7.4:25753586-25753605 None:intergenic 30.0%
AGGCCTTACTAAAAAGAAAA+AGG - chr7.4:25753619-25753638 MS.gene69067:CDS 30.0%
ATGTCTTCTTACCTATAACA+TGG + chr7.4:25753149-25753168 None:intergenic 30.0%
CAAGATAACAGAGCAAATTA+AGG + chr7.4:25753086-25753105 None:intergenic 30.0%
CATTAAGCTTAGTTAGATGT+AGG + chr7.4:25753313-25753332 None:intergenic 30.0%
CATTTACAATCCATCTGTTT+AGG - chr7.4:25753166-25753185 MS.gene69067:CDS 30.0%
TGATTGAAGATGCATCAAAA+TGG + chr7.4:25754616-25754635 None:intergenic 30.0%
TGTACGAATTTGTGAAACTT+TGG - chr7.4:25753414-25753433 MS.gene69067:CDS 30.0%
TTAAGCTTAGTTAGATGTAG+GGG + chr7.4:25753311-25753330 None:intergenic 30.0%
TTGGTATTTCCTACAACATA+TGG - chr7.4:25754409-25754428 MS.gene69067:intron 30.0%
! GAAAGCTATGCTTATCTTTT+TGG - chr7.4:25754590-25754609 MS.gene69067:CDS 30.0%
! GAAGTGTTTGTTACATTAGT+CGG - chr7.4:25754651-25754670 MS.gene69067:CDS 30.0%
!! AACTGAAATACAGTTTTGGT+TGG - chr7.4:25753954-25753973 MS.gene69067:intron 30.0%
AACAAGAACCATTACAGCAA+CGG + chr7.4:25754547-25754566 None:intergenic 35.0%
AACACGACCACCAATTATAT+AGG - chr7.4:25753576-25753595 MS.gene69067:CDS 35.0%
AATGCTGTAAAATGGTTTCG+TGG + chr7.4:25754165-25754184 None:intergenic 35.0%
AATTTCAAAGCAACACCTAC+AGG + chr7.4:25752865-25752884 None:intergenic 35.0%
ATGCTGTAAAATGGTTTCGT+GGG + chr7.4:25754164-25754183 None:intergenic 35.0%
ATTTCAAAGCAACACCTACA+GGG + chr7.4:25752864-25752883 None:intergenic 35.0%
CAAAACCAACAAATGGTTTG+GGG + chr7.4:25753287-25753306 None:intergenic 35.0%
GACAAAACCAACAAATGGTT+TGG + chr7.4:25753289-25753308 None:intergenic 35.0%
GACAATCGAATAAGCATTAC+AGG - chr7.4:25754222-25754241 MS.gene69067:intron 35.0%
GCTTTCTGTATACTAGTGAA+TGG + chr7.4:25754576-25754595 None:intergenic 35.0%
GGAAATACCAAGAAAGACAA+TGG + chr7.4:25754400-25754419 None:intergenic 35.0%
TACAAGTAATGAGCTAAGTG+AGG - chr7.4:25753599-25753618 MS.gene69067:CDS 35.0%
TATATCAAATCAAGCAGGAG+TGG + chr7.4:25753385-25753404 None:intergenic 35.0%
TATTGCGTATGGAGAAAGTT+TGG + chr7.4:25753251-25753270 None:intergenic 35.0%
TGTAATCGTAGGTATAGTCA+TGG + chr7.4:25754323-25754342 None:intergenic 35.0%
! GGACATGGATAATTGATAAC+TGG + chr7.4:25754440-25754459 None:intergenic 35.0%
AAAGGAAGCCTGAGATTACA+AGG - chr7.4:25752965-25752984 MS.gene69067:CDS 40.0%
AAATGTAAACACATCCTCCC+CGG + chr7.4:25752920-25752939 None:intergenic 40.0%
AACTTACCCAGATACTCGAA+AGG + chr7.4:25754032-25754051 None:intergenic 40.0%
ATGCTGAAATACTTGCAACC+GGG - chr7.4:25752899-25752918 MS.gene69067:CDS 40.0%
ATGTAAAGATGACAGCCTAC+CGG + chr7.4:25753030-25753049 None:intergenic 40.0%
CCACTTCACAAAGAACAGAA+TGG - chr7.4:25754462-25754481 MS.gene69067:intron 40.0%
CCATTCTGTTCTTTGTGAAG+TGG + chr7.4:25754465-25754484 None:intergenic 40.0%
CGAGTTTGATGAGAAGAATG+GGG - chr7.4:25753873-25753892 MS.gene69067:intron 40.0%
CGTATGGAGAAAGTTTGGTT+GGG + chr7.4:25753246-25753265 None:intergenic 40.0%
CTACAATGAAGATTATCCGC+CGG - chr7.4:25753008-25753027 MS.gene69067:CDS 40.0%
CTGGTTGTTCCATATGTTGT+AGG + chr7.4:25754421-25754440 None:intergenic 40.0%
CTGTTTCACGTATCCTTCAA+AGG - chr7.4:25752947-25752966 MS.gene69067:CDS 40.0%
GATGCTGAAATACTTGCAAC+CGG - chr7.4:25752898-25752917 MS.gene69067:CDS 40.0%
GATTGTCTTTCATTGCCCAA+GGG + chr7.4:25754209-25754228 None:intergenic 40.0%
GCGAGTTTGATGAGAAGAAT+GGG - chr7.4:25753872-25753891 MS.gene69067:intron 40.0%
GGCGGATAATCTTCATTGTA+GGG + chr7.4:25753009-25753028 None:intergenic 40.0%
GGTATAGTCATGGTCAAACT+CGG + chr7.4:25754313-25754332 None:intergenic 40.0%
GTATAGTCATGGTCAAACTC+GGG + chr7.4:25754312-25754331 None:intergenic 40.0%
GTCTCAACCATTGTCTTTCT+TGG - chr7.4:25754390-25754409 MS.gene69067:CDS 40.0%
GTTGCTGTAATGGTTCTTGT+TGG - chr7.4:25754546-25754565 MS.gene69067:CDS 40.0%
TAAAACTGCATAGCAGCAGT+GGG + chr7.4:25753674-25753693 None:intergenic 40.0%
TAATCTCAGGCTTCCTTTGA+AGG + chr7.4:25752963-25752982 None:intergenic 40.0%
TAGGGGACAAAACCAACAAA+TGG + chr7.4:25753294-25753313 None:intergenic 40.0%
TATAGTCATGGTCAAACTCG+GGG + chr7.4:25754311-25754330 None:intergenic 40.0%
TCAAACCCCAAACCATTTGT+TGG - chr7.4:25753279-25753298 MS.gene69067:CDS 40.0%
TCAGGAAACCTTGTAATCTC+AGG + chr7.4:25752976-25752995 None:intergenic 40.0%
TTAAAACTGCATAGCAGCAG+TGG + chr7.4:25753675-25753694 None:intergenic 40.0%
TTACAATCAGCACATGCGTA+AGG - chr7.4:25754338-25754357 MS.gene69067:intron 40.0%
TTCCAGGGAATGCTGTAAAA+TGG + chr7.4:25754173-25754192 None:intergenic 40.0%
TTCTCCATACGCAATAGTCA+GGG - chr7.4:25753255-25753274 MS.gene69067:CDS 40.0%
TTCTGTTCTTTGTGAAGTGG+CGG + chr7.4:25754462-25754481 None:intergenic 40.0%
TTGTAGGGAAAACTGTCATC+AGG + chr7.4:25752994-25753013 None:intergenic 40.0%
TTTCTCCATACGCAATAGTC+AGG - chr7.4:25753254-25753273 MS.gene69067:CDS 40.0%
! AACCATTTTACAGCATTCCC+TGG - chr7.4:25754168-25754187 MS.gene69067:intron 40.0%
! AGTGATTTCCAGTGTGGTTT+TGG - chr7.4:25754132-25754151 MS.gene69067:intron 40.0%
! ATTAGTCGGTGGACTTTCTA+TGG - chr7.4:25754665-25754684 MS.gene69067:CDS 40.0%
! GTGTTTGTTACATTAGTCGG+TGG - chr7.4:25754654-25754673 MS.gene69067:CDS 40.0%
! TCTATGGGGTGGTATTGTTT+TGG - chr7.4:25754681-25754700 MS.gene69067:CDS 40.0%
! TTAGTCGGTGGACTTTCTAT+GGG - chr7.4:25754666-25754685 MS.gene69067:CDS 40.0%
!! CATGTGCTGATTGTAATCGT+AGG + chr7.4:25754334-25754353 None:intergenic 40.0%
AAAACCGAACAACCCAAGCA+AGG + chr7.4:25754508-25754527 None:intergenic 45.0%
AAAACTGCATAGCAGCAGTG+GGG + chr7.4:25753673-25753692 None:intergenic 45.0%
AAAATGTTAGGAGTCGTCCG+TGG - chr7.4:25754004-25754023 MS.gene69067:intron 45.0%
AAGTAGGCTTCCAATCCCTT+GGG - chr7.4:25754191-25754210 MS.gene69067:intron 45.0%
AATACGTGGCCCTAAACAGA+TGG + chr7.4:25753179-25753198 None:intergenic 45.0%
AATGAAGATTATCCGCCGGT+AGG - chr7.4:25753012-25753031 MS.gene69067:CDS 45.0%
ACTCACCTTGAATGTCCAAC+CGG + chr7.4:25753215-25753234 None:intergenic 45.0%
ACTTCAGACTTCATCCCTGT+AGG - chr7.4:25752847-25752866 MS.gene69067:CDS 45.0%
CAGATACTCGAAAGGTTCCA+CGG + chr7.4:25754024-25754043 None:intergenic 45.0%
CGATTGTCTTTCATTGCCCA+AGG + chr7.4:25754210-25754229 None:intergenic 45.0%
CGGCGGATAATCTTCATTGT+AGG + chr7.4:25753010-25753029 None:intergenic 45.0%
GAAAACCGGTTGGACATTCA+AGG - chr7.4:25753207-25753226 MS.gene69067:CDS 45.0%
GCGTATGGAGAAAGTTTGGT+TGG + chr7.4:25753247-25753266 None:intergenic 45.0%
GGCGAGTTTGATGAGAAGAA+TGG - chr7.4:25753871-25753890 MS.gene69067:intron 45.0%
GGGGAGGATGTGTTTACATT+TGG - chr7.4:25752919-25752938 MS.gene69067:CDS 45.0%
TCTGTTCTTTGTGAAGTGGC+GGG + chr7.4:25754461-25754480 None:intergenic 45.0%
TCTTTCATTGCCCAAGGGAT+TGG + chr7.4:25754204-25754223 None:intergenic 45.0%
TGATCCCTGACTATTGCGTA+TGG + chr7.4:25753262-25753281 None:intergenic 45.0%
TGCTGAAATACTTGCAACCG+GGG - chr7.4:25752900-25752919 MS.gene69067:CDS 45.0%
TGGATGACAATGAGCTGCAT+TGG - chr7.4:25754262-25754281 MS.gene69067:intron 45.0%
TTACAGCATTCCCTGGAAGT+AGG - chr7.4:25754175-25754194 MS.gene69067:intron 45.0%
! GGAGTGTTAGAAGGTAAGTG+AGG + chr7.4:25753698-25753717 None:intergenic 45.0%
! GTGGAACCTTTCGAGTATCT+GGG - chr7.4:25754023-25754042 MS.gene69067:intron 45.0%
! TAGTCGGTGGACTTTCTATG+GGG - chr7.4:25754667-25754686 MS.gene69067:CDS 45.0%
! TGTTTGTGCCGTTGCTGTAA+TGG - chr7.4:25754536-25754555 MS.gene69067:intron 45.0%
!! TCAAGCATCGGAGTGTTAGA+AGG + chr7.4:25753707-25753726 None:intergenic 45.0%
AAACTGCATAGCAGCAGTGG+GGG + chr7.4:25753672-25753691 None:intergenic 50.0%
AGAATGGCCGCCTTTCTGTA+TGG - chr7.4:25754478-25754497 MS.gene69067:intron 50.0%
AGGAAACATGTTCACCCAGC+TGG - chr7.4:25754242-25754261 MS.gene69067:intron 50.0%
AGGACGACCATACAGAAAGG+CGG + chr7.4:25754488-25754507 None:intergenic 50.0%
CAGCTCATTGTCATCCAGCT+GGG + chr7.4:25754259-25754278 None:intergenic 50.0%
CTGCAGAGTGATTTCCAGTG+TGG - chr7.4:25754126-25754145 MS.gene69067:intron 50.0%
CTGTATGGTCGTCCTTGCTT+GGG - chr7.4:25754493-25754512 MS.gene69067:intron 50.0%
GAAGTAGGCTTCCAATCCCT+TGG - chr7.4:25754190-25754209 MS.gene69067:intron 50.0%
GCAAGGACGACCATACAGAA+AGG + chr7.4:25754491-25754510 None:intergenic 50.0%
GGATTGGAAGCCTACTTCCA+GGG + chr7.4:25754188-25754207 None:intergenic 50.0%
GGCACTTTCCAAAACCACAC+TGG + chr7.4:25754143-25754162 None:intergenic 50.0%
TAAAGATGACAGCCTACCGG+CGG + chr7.4:25753027-25753046 None:intergenic 50.0%
TCTCATCAAACTCGCCACGT+AGG + chr7.4:25753867-25753886 None:intergenic 50.0%
TCTGTATGGTCGTCCTTGCT+TGG - chr7.4:25754492-25754511 MS.gene69067:intron 50.0%
TGAAATACTTGCAACCGGGG+AGG - chr7.4:25752903-25752922 MS.gene69067:CDS 50.0%
! CGTGGAACCTTTCGAGTATC+TGG - chr7.4:25754022-25754041 MS.gene69067:intron 50.0%
! TCGTCCTTGCTTGGGTTGTT+CGG - chr7.4:25754501-25754520 MS.gene69067:intron 50.0%
!! AATGTCCAACCGGTTTTCCG+AGG + chr7.4:25753205-25753224 None:intergenic 50.0%
!! ATGTCCAACCGGTTTTCCGA+GGG + chr7.4:25753204-25753223 None:intergenic 50.0%
AAACTCGCCACGTAGGAGGT+GGG + chr7.4:25753860-25753879 None:intergenic 55.0%
AACTGCATAGCAGCAGTGGG+GGG + chr7.4:25753671-25753690 None:intergenic 55.0%
CATCAAACTCGCCACGTAGG+AGG + chr7.4:25753864-25753883 None:intergenic 55.0%
CGTATTCGCCCTCGGAAAAC+CGG - chr7.4:25753193-25753212 MS.gene69067:CDS 55.0%
CGTTTAACCCACCTCCTACG+TGG - chr7.4:25753850-25753869 MS.gene69067:intron 55.0%
GCAGCTCATTGTCATCCAGC+TGG + chr7.4:25754260-25754279 None:intergenic 55.0%
GGGATTGGAAGCCTACTTCC+AGG + chr7.4:25754189-25754208 None:intergenic 55.0%
! CTTTGTGAAGTGGCGGGACA+TGG + chr7.4:25754455-25754474 None:intergenic 55.0%
! GTTTTCCGAGGGCGAATACG+TGG + chr7.4:25753193-25753212 None:intergenic 55.0%
! TCGGTGGACTTTCTATGGGG+TGG - chr7.4:25754670-25754689 MS.gene69067:CDS 55.0%
!! CGTAGGAGGTGGGTTAAACG+AGG + chr7.4:25753850-25753869 None:intergenic 55.0%
CAAACTCGCCACGTAGGAGG+TGG + chr7.4:25753861-25753880 None:intergenic 60.0%
TAGGGCCACGTATTCGCCCT+CGG - chr7.4:25753185-25753204 MS.gene69067:CDS 60.0%
TTCGCCCTCGGAAAACCGGT+TGG - chr7.4:25753197-25753216 MS.gene69067:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 25752835 25754734 25752835 ID=MS.gene69067
chr7.4 mRNA 25752835 25754734 25752835 ID=MS.gene69067.t1;Parent=MS.gene69067
chr7.4 exon 25754540 25754734 25754540 ID=MS.gene69067.t1.exon1;Parent=MS.gene69067.t1
chr7.4 CDS 25754540 25754734 25754540 ID=cds.MS.gene69067.t1;Parent=MS.gene69067.t1
chr7.4 exon 25754341 25754412 25754341 ID=MS.gene69067.t1.exon2;Parent=MS.gene69067.t1
chr7.4 CDS 25754341 25754412 25754341 ID=cds.MS.gene69067.t1;Parent=MS.gene69067.t1
chr7.4 exon 25753525 25753731 25753525 ID=MS.gene69067.t1.exon3;Parent=MS.gene69067.t1
chr7.4 CDS 25753525 25753731 25753525 ID=cds.MS.gene69067.t1;Parent=MS.gene69067.t1
chr7.4 exon 25752835 25753437 25752835 ID=MS.gene69067.t1.exon4;Parent=MS.gene69067.t1
chr7.4 CDS 25752835 25753437 25752835 ID=cds.MS.gene69067.t1;Parent=MS.gene69067.t1
Gene Sequence

>MS.gene69067

ATGGTTTTGGAAACTTCAGACTTCATCCCTGTAGGTGTTGCTTTGAAATTTTCAGACTCATATGATGCTGAAATACTTGCAACCGGGGAGGATGTGTTTACATTTGGAGAGACTGTTTCACGTATCCTTCAAAGGAAGCCTGAGATTACAAGGTTTCCTGATGACAGTTTTCCCTACAATGAAGATTATCCGCCGGTAGGCTGTCATCTTTACATCCATTCTAACTGTTTTTCTTCTACAACCATTATCCTTAATTTGCTCTGTTATCTTGTTCACTTCTGCCTAATTTTTGTCCTCTATTTTTTTGATTACCATGTTATAGGTAAGAAGACATTTACAATCCATCTGTTTAGGGCCACGTATTCGCCCTCGGAAAACCGGTTGGACATTCAAGGTGAGTTCTCTGCTCCCAACCAAACTTTCTCCATACGCAATAGTCAGGGATCAAACCCCAAACCATTTGTTGGTTTTGTCCCCTACATCTAACTAAGCTTAATGTTATTTTCCTAAAATAGTTTTCAATGTTTTGGTTACAAATTGTTAGCTTCCACTCCTGCTTGATTTGATATACTGTTTTTCTGTACGAATTTGTGAAACTTTGGTGAGTTATCATTTCATTGATTCTCTGATATGCTATATGAATTTTTCAAAATGAAGTAATCAAGAGAGAGAACCAAATATTGTGATTCATTGTGAATTAACTTTCTAATTATTGATTGACCAACTAATAGTAGTACAATGAACACGACCACCAATTATATAGGTACAAGTAATGAGCTAAGTGAGGCCTTACTAAAAAGAAAAAGGAAAACAGAAATGGAATAGAAGCAAAGCCCCCCACTGCTGCTATGCAGTTTTAACCTCACTTACCTTCTAACACTCCGATGCTTGATAATGTTTTTACATAATTTTGATTATAAGCAGATTATTAATGATTTTGCAATAAAAACTACACGACGATATTTTACAAGTATAATGAAGATAATATTCAAATAATTGATAGGTATCATTTCCTCGTTTAACCCACCTCCTACGTGGCGAGTTTGATGAGAAGAATGGGGTTGCTGAAGCAAAACAAAGATTAGCTCAATATATAAGGAGCAAGCATGCTAACAAGAAAACTGAAATACAGTTTTGGTTGGATATAGAAGAAATATTATCTTGCTACAAAAATGTTAGGAGTCGTCCGTGGAACCTTTCGAGTATCTGGGTAAGTTGATAAATTTTTTGCTTTATATGATTTATTACTACTACTACTATTATTATTATTTATATTTTGTTTCATTTCATACTGCAGAGTGATTTCCAGTGTGGTTTTGGAAAGTGCCCACGAAACCATTTTACAGCATTCCCTGGAAGTAGGCTTCCAATCCCTTGGGCAATGAAAGACAATCGAATAAGCATTACAGGAAACATGTTCACCCAGCTGGATGACAATGAGCTGCATTGGTCTAATAGAAACATATCAAGAGACCCCGAGTTTGACCATGACTATACCTACGATTACAATCAGCACATGCGTAAGGAAATCGTGCGATGTCTCTCATCTTTTGTAGTCTCAACCATTGTCTTTCTTGGTATTTCCTACAACATATGGAACAACCAGTTATCAATTATCCATGTCCCGCCACTTCACAAAGAACAGAATGGCCGCCTTTCTGTATGGTCGTCCTTGCTTGGGTTGTTCGGTTTTATGATGTTTGTTTGTGCCGTTGCTGTAATGGTTCTTGTTGGTGCACCATTCACTAGTATACAGAAAGCTATGCTTATCTTTTTGGCCATTTTGATGCATCTTCAATCAGCTAATTCAATCATCGAAGTGTTTGTTACATTAGTCGGTGGACTTTCTATGGGGTGGTATTGTTTTGGAAAAAAACAACCACCAAATGAAGTGATATGA
Protein sequence

>MS.gene69067.t1

MVLETSDFIPVGVALKFSDSYDAEILATGEDVFTFGETVSRILQRKPEITRFPDDSFPYNEDYPPVRRHLQSICLGPRIRPRKTGWTFKVSFPRLTHLLRGEFDEKNGVAEAKQRLAQYIRSKHANKKTEIQFWLDIEEILSCYKNVRSRPWNLSSIWSDFQCGFGKCPRNHFTAFPGSRLPIPWAMKDNRISITGNMFTQLDDNELHWSNRNISRDPEFDHDYTYDYNQHMRKEIVRCLSSFVVSTIVFLGISYNIWNNQLSIIHVPPLHKEQNGRLSVWSSLLGLFGFMMFVCAVAVMVLVGAPFTSIQKAMLIFLAILMHLQSANSIIEVFVTLVGGLSMGWYCFGKKQPPNEVI