AlfalfaGEDB | MS.gene69289

M. sativa cultivar XinJiangDaYe / MS.gene69289

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69289.t1	XP_003600807.1	97.6	170	4	0	1	170	1	170	4.70E-79	303.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69289.t1	G7JAJ4	97.6	170	4	0	1	170	1	170	3.4e-79	303.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057356	MS.gene69289	0.818785	1.63E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69289.t1	MTR_3g069580	97.647	170	4	0	1	170	1	170	1.76e-116	327
MS.gene69289.t1	MTR_4g064923	51.977	177	70	2	8	170	12	187	6.27e-46	149
MS.gene69289.t1	MTR_7g051210	47.879	165	79	3	10	169	33	195	7.86e-31	110
MS.gene69289.t1	MTR_7g112930	80.952	42	8	0	20	61	33	74	7.77e-17	71.6
MS.gene69289.t1	MTR_1g020950	42.857	84	48	0	14	97	3	86	2.96e-16	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69289.t1	AT5G05950	65.318	173	50	2	8	170	3	175	1.20e-73	219
MS.gene69289.t1	AT3G07510	46.626	163	82	3	10	168	26	187	7.46e-35	120
MS.gene69289.t1	AT3G07510	46.626	163	82	3	10	168	26	187	7.46e-35	120
MS.gene69289.t1	AT3G07510	46.626	163	82	3	10	168	36	197	1.34e-34	120
MS.gene69289.t1	AT3G46890	47.826	184	73	6	8	168	19	202	1.16e-33	118
MS.gene69289.t1	AT2G01580	42.593	162	86	4	14	170	32	191	4.98e-30	108

Find 42 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGTTGGGATGCTTGTTTC+TGG	0.140160	3.2:-63382428	MS.gene69289:CDS
TCTCATGTTGCTCATGTTAA+TGG	0.182345	3.2:-63382461	MS.gene69289:CDS
GGAGGGGTCGTTTGGGTTGA+AGG	0.327827	3.2:+63382634	None:intergenic
AGAGTGTTGGAGGGGTCGTT+TGG	0.330242	3.2:+63382626	None:intergenic
AACATAAACACAAAGCGAAA+TGG	0.369271	3.2:+63382274	None:intergenic
GAGTTGGGATGCTTGTTTCT+GGG	0.377021	3.2:-63382427	MS.gene69289:CDS
GGGATGCTTGTTTCTGGGAT+TGG	0.380945	3.2:-63382422	MS.gene69289:CDS
GTCTGTGTTGAGAGTGTTGG+AGG	0.414551	3.2:+63382616	None:intergenic
GAGTGTTGGAGGGGTCGTTT+GGG	0.416230	3.2:+63382627	None:intergenic
TTGTGGGAGTCAACATGCTT+TGG	0.419379	3.2:-63382327	MS.gene69289:CDS
GGGGTCTGTGTTGAGAGTGT+TGG	0.425880	3.2:+63382613	None:intergenic
AGCTTGGGACTGTTGCTTGT+GGG	0.440350	3.2:-63382343	MS.gene69289:CDS
AATGTTGTTCAGATTAAGCT+TGG	0.444641	3.2:-63382359	MS.gene69289:CDS
GGCGGTGGTGGGGACACAGG+AGG	0.444733	3.2:+63382592	None:intergenic
ACATAAACACAAAGCGAAAT+GGG	0.462286	3.2:+63382275	None:intergenic
TACATTTGTGATTGCGTCGA+GGG	0.464984	3.2:+63382694	None:intergenic
GGTCGTTTGGGTTGAAGGAG+AGG	0.478484	3.2:+63382639	None:intergenic
CGGTGGTGGGGACACAGGAG+GGG	0.494754	3.2:+63382594	None:intergenic
AAGCTTGGGACTGTTGCTTG+TGG	0.497800	3.2:-63382344	MS.gene69289:CDS
TCTGTGTTGAGAGTGTTGGA+GGG	0.501722	3.2:+63382617	None:intergenic
TTTCGGCGATGGCGGTGGTG+GGG	0.508820	3.2:+63382582	None:intergenic
GCGGTGGTGGGGACACAGGA+GGG	0.509505	3.2:+63382593	None:intergenic
ATGTTGTTCAGATTAAGCTT+GGG	0.515905	3.2:-63382358	MS.gene69289:CDS
GATGGCGGTGGTGGGGACAC+AGG	0.517533	3.2:+63382589	None:intergenic
TTCACCCTCGGCGTATTCAT+CGG	0.533899	3.2:-63382662	MS.gene69289:CDS
ATGGATCTGTGCTAAGAGTT+GGG	0.538632	3.2:-63382442	MS.gene69289:CDS
AATGGATCTGTGCTAAGAGT+TGG	0.551878	3.2:-63382443	MS.gene69289:CDS
GGAAGTTCTTGAAAGACGAA+TGG	0.555122	3.2:+63382484	None:intergenic
GAATACGCCGAGGGTGAAGA+GGG	0.557211	3.2:+63382667	None:intergenic
TTGCGTCGAGGGCTGTTATG+TGG	0.571255	3.2:+63382705	None:intergenic
GTAAACTCCCTCTTCACCCT+CGG	0.571510	3.2:-63382674	MS.gene69289:CDS
CGCTGGTGATTGATGATGAG+AGG	0.576756	3.2:+63382729	None:intergenic
GAGCGATAAGACTAGAGAAG+AGG	0.579122	3.2:+63382522	None:intergenic
TGAATACGCCGAGGGTGAAG+AGG	0.585530	3.2:+63382666	None:intergenic
TTACATTTGTGATTGCGTCG+AGG	0.616844	3.2:+63382693	None:intergenic
CTGTGTTGAGAGTGTTGGAG+GGG	0.659395	3.2:+63382618	None:intergenic
ATTAACATGAGCAACATGAG+AGG	0.669628	3.2:+63382463	None:intergenic
GAGGCCGATGAATACGCCGA+GGG	0.676389	3.2:+63382658	None:intergenic
GCTGGTGATTGATGATGAGA+GGG	0.682648	3.2:+63382730	None:intergenic
GAGGGCTGTTATGTGGACGC+TGG	0.684505	3.2:+63382712	None:intergenic
AGAGGCCGATGAATACGCCG+AGG	0.689486	3.2:+63382657	None:intergenic
AGTATACATGAAATGCGACG+AGG	0.750291	3.2:+63382558	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACATAAACACAAAGCGAAA+TGG	+	chr3.2:63382707-63382726	None:intergenic	30.0%
	AATGTTGTTCAGATTAAGCT+TGG	-	chr3.2:63382619-63382638	MS.gene69289:CDS	30.0%
	ACATAAACACAAAGCGAAAT+GGG	+	chr3.2:63382706-63382725	None:intergenic	30.0%
	ATGGGAACAAAAACAAAAAG+CGG	+	chr3.2:63382688-63382707	None:intergenic	30.0%
	ATGTTGTTCAGATTAAGCTT+GGG	-	chr3.2:63382620-63382639	MS.gene69289:CDS	30.0%
	ATTAACATGAGCAACATGAG+AGG	+	chr3.2:63382518-63382537	None:intergenic	35.0%
	TCTCATGTTGCTCATGTTAA+TGG	-	chr3.2:63382517-63382536	MS.gene69289:CDS	35.0%
	AATGGATCTGTGCTAAGAGT+TGG	-	chr3.2:63382535-63382554	MS.gene69289:CDS	40.0%
	AGTATACATGAAATGCGACG+AGG	+	chr3.2:63382423-63382442	None:intergenic	40.0%
	ATGGATCTGTGCTAAGAGTT+GGG	-	chr3.2:63382536-63382555	MS.gene69289:CDS	40.0%
	GGAAGTTCTTGAAAGACGAA+TGG	+	chr3.2:63382497-63382516	None:intergenic	40.0%
	TACATTTGTGATTGCGTCGA+GGG	+	chr3.2:63382287-63382306	None:intergenic	40.0%
	TTACATTTGTGATTGCGTCG+AGG	+	chr3.2:63382288-63382307	None:intergenic	40.0%
!	ATGAAATGCGACGAGGTTTT+CGG	+	chr3.2:63382416-63382435	None:intergenic	40.0%
!!	TCTGGGATTGGATCTGTTTT+AGG	-	chr3.2:63382568-63382587	MS.gene69289:CDS	40.0%
	AGAGTTGGGATGCTTGTTTC+TGG	-	chr3.2:63382550-63382569	MS.gene69289:CDS	45.0%
	GAGCGATAAGACTAGAGAAG+AGG	+	chr3.2:63382459-63382478	None:intergenic	45.0%
	TTGTGGGAGTCAACATGCTT+TGG	-	chr3.2:63382651-63382670	MS.gene69289:CDS	45.0%
!	GAGTTGGGATGCTTGTTTCT+GGG	-	chr3.2:63382551-63382570	MS.gene69289:CDS	45.0%
!!	GCTGGTGATTGATGATGAGA+GGG	+	chr3.2:63382251-63382270	None:intergenic	45.0%
!!	TCTGTGTTGAGAGTGTTGGA+GGG	+	chr3.2:63382364-63382383	None:intergenic	45.0%
	GTAAACTCCCTCTTCACCCT+CGG	-	chr3.2:63382304-63382323	MS.gene69289:CDS	50.0%
	TTCACCCTCGGCGTATTCAT+CGG	-	chr3.2:63382316-63382335	MS.gene69289:CDS	50.0%
!	AAGCTTGGGACTGTTGCTTG+TGG	-	chr3.2:63382634-63382653	MS.gene69289:CDS	50.0%
!	AGCTTGGGACTGTTGCTTGT+GGG	-	chr3.2:63382635-63382654	MS.gene69289:CDS	50.0%
!!	CGCTGGTGATTGATGATGAG+AGG	+	chr3.2:63382252-63382271	None:intergenic	50.0%
!!	CTGTGTTGAGAGTGTTGGAG+GGG	+	chr3.2:63382363-63382382	None:intergenic	50.0%
!!	GGGATGCTTGTTTCTGGGAT+TGG	-	chr3.2:63382556-63382575	MS.gene69289:CDS	50.0%
!!	GTCTGTGTTGAGAGTGTTGG+AGG	+	chr3.2:63382365-63382384	None:intergenic	50.0%
	GAATACGCCGAGGGTGAAGA+GGG	+	chr3.2:63382314-63382333	None:intergenic	55.0%
	TGAATACGCCGAGGGTGAAG+AGG	+	chr3.2:63382315-63382334	None:intergenic	55.0%
	TTGCGTCGAGGGCTGTTATG+TGG	+	chr3.2:63382276-63382295	None:intergenic	55.0%
!	GGGGTCTGTGTTGAGAGTGT+TGG	+	chr3.2:63382368-63382387	None:intergenic	55.0%
!!	AGAGTGTTGGAGGGGTCGTT+TGG	+	chr3.2:63382355-63382374	None:intergenic	55.0%
!!	GAGTGTTGGAGGGGTCGTTT+GGG	+	chr3.2:63382354-63382373	None:intergenic	55.0%
!!	GGTCGTTTGGGTTGAAGGAG+AGG	+	chr3.2:63382342-63382361	None:intergenic	55.0%
	AGAGGCCGATGAATACGCCG+AGG	+	chr3.2:63382324-63382343	None:intergenic	60.0%
	GAGGCCGATGAATACGCCGA+GGG	+	chr3.2:63382323-63382342	None:intergenic	60.0%
	GAGGGCTGTTATGTGGACGC+TGG	+	chr3.2:63382269-63382288	None:intergenic	60.0%
!	GACGAGGTTTTCGGCGATGG+CGG	+	chr3.2:63382407-63382426	None:intergenic	60.0%
!	TGCGACGAGGTTTTCGGCGA+TGG	+	chr3.2:63382410-63382429	None:intergenic	60.0%
!!	GGAGGGGTCGTTTGGGTTGA+AGG	+	chr3.2:63382347-63382366	None:intergenic	60.0%
!!	TTTTCGGCGATGGCGGTGGT+GGG	+	chr3.2:63382400-63382419	None:intergenic	60.0%
!	TTTCGGCGATGGCGGTGGTG+GGG	+	chr3.2:63382399-63382418	None:intergenic	65.0%
!!	GAGGTTTTCGGCGATGGCGG+TGG	+	chr3.2:63382404-63382423	None:intergenic	65.0%
!!	GTTTTCGGCGATGGCGGTGG+TGG	+	chr3.2:63382401-63382420	None:intergenic	65.0%
!!	CGGTGGTGGGGACACAGGAG+GGG	+	chr3.2:63382387-63382406	None:intergenic	70.0%
!!	GATGGCGGTGGTGGGGACAC+AGG	+	chr3.2:63382392-63382411	None:intergenic	70.0%
!!	GCGGTGGTGGGGACACAGGA+GGG	+	chr3.2:63382388-63382407	None:intergenic	70.0%
!!	GGCGGTGGTGGGGACACAGG+AGG	+	chr3.2:63382389-63382408	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	63382244	63382756	63382244	ID=MS.gene69289
chr3.2	mRNA	63382244	63382756	63382244	ID=MS.gene69289.t1;Parent=MS.gene69289
chr3.2	exon	63382244	63382756	63382244	ID=MS.gene69289.t1.exon1;Parent=MS.gene69289.t1
chr3.2	CDS	63382244	63382756	63382244	ID=cds.MS.gene69289.t1;Parent=MS.gene69289.t1

Gene Sequence

>MS.gene69289

ATGACCCTCTCATCATCAATCACCAGCGTCCACATAACAGCCCTCGACGCAATCACAAATGTAAACTCCCTCTTCACCCTCGGCGTATTCATCGGCCTCTCCTTCAACCCAAACGACCCCTCCAACACTCTCAACACAGACCCCTCCTGTGTCCCCACCACCGCCATCGCCGAAAACCTCGTCGCATTTCATGTATACTCATTCAGCTCATTCCTCTTCTCTAGTCTTATCGCTCTTGCACTCAAACAAACCATTCGTCTTTCAAGAACTTCCTCTCATGTTGCTCATGTTAATGGATCTGTGCTAAGAGTTGGGATGCTTGTTTCTGGGATTGGATCTGTTTTAGGATGTGCTTTTCTTATGCTTGCTTTAGTGAATGTTGTTCAGATTAAGCTTGGGACTGTTGCTTGTGGGAGTCAACATGCTTTGGCTGCTGTTGTTCCGCTTTTTGTTTTTGTTCCCATTTCGCTTTGTGTTTATGTTACTGTTGTTGTTTATGCTTTTACTCGCTAG

Protein sequence

>MS.gene69289.t1

MTLSSSITSVHITALDAITNVNSLFTLGVFIGLSFNPNDPSNTLNTDPSCVPTTAIAENLVAFHVYSFSSFLFSSLIALALKQTIRLSRTSSHVAHVNGSVLRVGMLVSGIGSVLGCAFLMLALVNVVQIKLGTVACGSQHALAAVVPLFVFVPISLCVYVTVVVYAFTR