Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057356.t1 | XP_003600807.1 | 97.6 | 170 | 4 | 0 | 1 | 170 | 1 | 170 | 4.70E-79 | 303.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057356.t1 | G7JAJ4 | 97.6 | 170 | 4 | 0 | 1 | 170 | 1 | 170 | 3.4e-79 | 303.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene057356 | 0.818225 | 2.19E-52 | -1.69E-46 |
| MS.gene050710 | MS.gene057356 | 0.821203 | 4.58E-53 | -1.69E-46 |
| MS.gene053108 | MS.gene057356 | 0.802385 | 5.75E-49 | -1.69E-46 |
| MS.gene054941 | MS.gene057356 | 0.809773 | 1.60E-50 | -1.69E-46 |
| MS.gene05652 | MS.gene057356 | 0.804016 | 2.64E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene059717 | -0.803508 | 3.37E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene06421 | 0.811568 | 6.56E-51 | -1.69E-46 |
| MS.gene057356 | MS.gene065682 | 0.835089 | 2.06E-56 | -1.69E-46 |
| MS.gene057356 | MS.gene068946 | -0.816518 | 5.31E-52 | -1.69E-46 |
| MS.gene057356 | MS.gene22760 | 0.808578 | 2.89E-50 | -1.69E-46 |
| MS.gene057356 | MS.gene25726 | 0.803547 | 3.31E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene27560 | 0.827755 | 1.31E-54 | -1.69E-46 |
| MS.gene057356 | MS.gene29968 | -0.825296 | 5.06E-54 | -1.69E-46 |
| MS.gene057356 | MS.gene31159 | -0.811124 | 8.19E-51 | -1.69E-46 |
| MS.gene057356 | MS.gene31178 | -0.806849 | 6.74E-50 | -1.69E-46 |
| MS.gene057356 | MS.gene32932 | 0.811426 | 7.04E-51 | -1.69E-46 |
| MS.gene057356 | MS.gene34207 | 0.809134 | 2.20E-50 | -1.69E-46 |
| MS.gene057356 | MS.gene35794 | 0.828041 | 1.12E-54 | -1.69E-46 |
| MS.gene057356 | MS.gene35795 | 0.835585 | 1.54E-56 | -1.69E-46 |
| MS.gene057356 | MS.gene35853 | 0.827926 | 1.19E-54 | -1.69E-46 |
| MS.gene057356 | MS.gene35931 | 0.836486 | 9.10E-57 | -1.69E-46 |
| MS.gene057356 | MS.gene36105 | -0.807888 | 4.06E-50 | -1.69E-46 |
| MS.gene057356 | MS.gene36820 | 0.811723 | 6.07E-51 | -1.69E-46 |
| MS.gene057356 | MS.gene38102 | 0.836825 | 7.46E-57 | -1.69E-46 |
| MS.gene057356 | MS.gene38166 | 0.821914 | 3.13E-53 | -1.69E-46 |
| MS.gene057356 | MS.gene40278 | 0.814614 | 1.41E-51 | -1.69E-46 |
| MS.gene057356 | MS.gene40724 | 0.823899 | 1.08E-53 | -1.69E-46 |
| MS.gene057356 | MS.gene41190 | 0.801758 | 7.74E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene43727 | 0.812315 | 4.51E-51 | -1.69E-46 |
| MS.gene057356 | MS.gene44835 | 0.822959 | 1.79E-53 | -1.69E-46 |
| MS.gene057356 | MS.gene50066 | 0.804936 | 1.70E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene52042 | -0.808198 | 3.48E-50 | -1.69E-46 |
| MS.gene057356 | MS.gene52363 | 0.801174 | 1.02E-48 | -1.69E-46 |
| MS.gene057356 | MS.gene54461 | 0.801922 | 7.16E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene57731 | 0.820709 | 5.94E-53 | -1.69E-46 |
| MS.gene057356 | MS.gene58849 | 0.804706 | 1.90E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene69289 | 0.818785 | 1.63E-52 | -1.69E-46 |
| MS.gene057356 | MS.gene69607 | 0.810434 | 1.16E-50 | -1.69E-46 |
| MS.gene057356 | MS.gene70491 | 0.803154 | 3.99E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene71690 | 0.812958 | 3.26E-51 | -1.69E-46 |
| MS.gene057356 | MS.gene71924 | 0.824929 | 6.18E-54 | -1.69E-46 |
| MS.gene057356 | MS.gene73893 | 0.807381 | 5.20E-50 | -1.69E-46 |
| MS.gene057356 | MS.gene74029 | 0.826343 | 2.86E-54 | -1.69E-46 |
| MS.gene057356 | MS.gene74253 | 0.819871 | 9.25E-53 | -1.69E-46 |
| MS.gene057356 | MS.gene75179 | 0.836093 | 1.15E-56 | -1.69E-46 |
| MS.gene057356 | MS.gene76037 | 0.804922 | 1.71E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene76902 | 0.802954 | 4.39E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene82050 | -0.847012 | 1.51E-59 | -1.69E-46 |
| MS.gene057356 | MS.gene82768 | 0.805275 | 1.44E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene85150 | 0.817662 | 2.94E-52 | -1.69E-46 |
| MS.gene057356 | MS.gene89264 | 0.802749 | 4.84E-49 | -1.69E-46 |
| MS.gene057356 | MS.gene90842 | 0.818835 | 1.59E-52 | -1.69E-46 |
| MS.gene057356 | MS.gene90899 | 0.815885 | 7.35E-52 | -1.69E-46 |
| MS.gene057356 | MS.gene91342 | 0.811774 | 5.92E-51 | -1.69E-46 |
| MS.gene057356 | MS.gene92996 | 0.828704 | 7.76E-55 | -1.69E-46 |
| MS.gene057356 | MS.gene93402 | 0.820934 | 5.28E-53 | -1.69E-46 |
| MS.gene057356 | MS.gene99885 | 0.815336 | 9.74E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057356.t1 | MTR_3g069580 | 97.647 | 170 | 4 | 0 | 1 | 170 | 1 | 170 | 1.76e-116 | 327 |
| MS.gene057356.t1 | MTR_4g064923 | 51.977 | 177 | 70 | 2 | 8 | 170 | 12 | 187 | 6.27e-46 | 149 |
| MS.gene057356.t1 | MTR_7g051210 | 47.879 | 165 | 79 | 3 | 10 | 169 | 33 | 195 | 7.86e-31 | 110 |
| MS.gene057356.t1 | MTR_7g112930 | 80.952 | 42 | 8 | 0 | 20 | 61 | 33 | 74 | 7.77e-17 | 71.6 |
| MS.gene057356.t1 | MTR_1g020950 | 42.857 | 84 | 48 | 0 | 14 | 97 | 3 | 86 | 2.96e-16 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057356.t1 | AT5G05950 | 65.318 | 173 | 50 | 2 | 8 | 170 | 3 | 175 | 1.20e-73 | 219 |
| MS.gene057356.t1 | AT3G07510 | 46.626 | 163 | 82 | 3 | 10 | 168 | 26 | 187 | 7.46e-35 | 120 |
| MS.gene057356.t1 | AT3G07510 | 46.626 | 163 | 82 | 3 | 10 | 168 | 26 | 187 | 7.46e-35 | 120 |
| MS.gene057356.t1 | AT3G07510 | 46.626 | 163 | 82 | 3 | 10 | 168 | 36 | 197 | 1.34e-34 | 120 |
| MS.gene057356.t1 | AT3G46890 | 47.826 | 184 | 73 | 6 | 8 | 168 | 19 | 202 | 1.16e-33 | 118 |
| MS.gene057356.t1 | AT2G01580 | 42.593 | 162 | 86 | 4 | 14 | 170 | 32 | 191 | 4.98e-30 | 108 |
Find 41 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGTTGGGATGCTTGTTTC+TGG | 0.140160 | 3.1:-56862669 | MS.gene057356:CDS |
| TCTCATGTTGCTCATGTTAA+TGG | 0.182345 | 3.1:-56862702 | MS.gene057356:CDS |
| TACATTTGTGATTGCGTCTA+AGG | 0.246912 | 3.1:+56862935 | None:intergenic |
| GGAGGGGTCGTTTGGGTTGA+AGG | 0.327827 | 3.1:+56862875 | None:intergenic |
| AGAGTGTTGGAGGGGTCGTT+TGG | 0.330242 | 3.1:+56862867 | None:intergenic |
| AACATAAACACAAAGCGAAA+TGG | 0.369271 | 3.1:+56862515 | None:intergenic |
| GAGTTGGGATGCTTGTTTCT+GGG | 0.377021 | 3.1:-56862668 | MS.gene057356:CDS |
| GGGATGCTTGTTTCTGGGAT+TGG | 0.380945 | 3.1:-56862663 | MS.gene057356:CDS |
| GTCTGTGTTGAGAGTGTTGG+AGG | 0.414551 | 3.1:+56862857 | None:intergenic |
| GAGTGTTGGAGGGGTCGTTT+GGG | 0.416230 | 3.1:+56862868 | None:intergenic |
| TTGTGGGAGTCAACATGCTT+TGG | 0.419379 | 3.1:-56862568 | MS.gene057356:CDS |
| GGGGTCTGTGTTGAGAGTGT+TGG | 0.425880 | 3.1:+56862854 | None:intergenic |
| AGCTTGGGACTGTTGCTTGT+GGG | 0.440350 | 3.1:-56862584 | MS.gene057356:CDS |
| GGCGGTGGTGGGGACACAGG+AGG | 0.444733 | 3.1:+56862833 | None:intergenic |
| ACATAAACACAAAGCGAAAT+GGG | 0.462286 | 3.1:+56862516 | None:intergenic |
| GGTCGTTTGGGTTGAAGGAG+AGG | 0.478484 | 3.1:+56862880 | None:intergenic |
| CGGTGGTGGGGACACAGGAG+GGG | 0.494754 | 3.1:+56862835 | None:intergenic |
| AAGCTTGGGACTGTTGCTTG+TGG | 0.497800 | 3.1:-56862585 | MS.gene057356:CDS |
| TCTGTGTTGAGAGTGTTGGA+GGG | 0.501722 | 3.1:+56862858 | None:intergenic |
| TTTCGGCGATGGCGGTGGTG+GGG | 0.508820 | 3.1:+56862823 | None:intergenic |
| GCGGTGGTGGGGACACAGGA+GGG | 0.509505 | 3.1:+56862834 | None:intergenic |
| GATGGCGGTGGTGGGGACAC+AGG | 0.517533 | 3.1:+56862830 | None:intergenic |
| TTCACCCTCGGCGTATTCAT+CGG | 0.533899 | 3.1:-56862903 | MS.gene057356:CDS |
| ATGGATCTGTGCTAAGAGTT+GGG | 0.538632 | 3.1:-56862683 | MS.gene057356:CDS |
| AATGGATCTGTGCTAAGAGT+TGG | 0.551878 | 3.1:-56862684 | MS.gene057356:CDS |
| AATGTTGTGCAGATTAAGCT+TGG | 0.554598 | 3.1:-56862600 | MS.gene057356:CDS |
| GGAAGTTCTTGAAAGACGAA+TGG | 0.555122 | 3.1:+56862725 | None:intergenic |
| GAATACGCCGAGGGTGAAGA+GGG | 0.557211 | 3.1:+56862908 | None:intergenic |
| GTAAACTCCCTCTTCACCCT+CGG | 0.571510 | 3.1:-56862915 | MS.gene057356:CDS |
| CGCTGGTGATTGATGATGAG+AGG | 0.576756 | 3.1:+56862970 | None:intergenic |
| GAGCGATAAGACTAGAGAAG+AGG | 0.579122 | 3.1:+56862763 | None:intergenic |
| TGAATACGCCGAGGGTGAAG+AGG | 0.585530 | 3.1:+56862907 | None:intergenic |
| ATGTTGTGCAGATTAAGCTT+GGG | 0.608953 | 3.1:-56862599 | MS.gene057356:CDS |
| TTGCGTCTAAGGCTGTTATG+TGG | 0.639259 | 3.1:+56862946 | None:intergenic |
| TAAGGCTGTTATGTGGACGC+TGG | 0.648140 | 3.1:+56862953 | None:intergenic |
| CTGTGTTGAGAGTGTTGGAG+GGG | 0.659395 | 3.1:+56862859 | None:intergenic |
| ATTAACATGAGCAACATGAG+AGG | 0.669628 | 3.1:+56862704 | None:intergenic |
| GAGGCCGATGAATACGCCGA+GGG | 0.676389 | 3.1:+56862899 | None:intergenic |
| GCTGGTGATTGATGATGAGA+GGG | 0.682648 | 3.1:+56862971 | None:intergenic |
| AGAGGCCGATGAATACGCCG+AGG | 0.689486 | 3.1:+56862898 | None:intergenic |
| AGTATACATGAAATGCGACG+AGG | 0.750291 | 3.1:+56862799 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATAAACACAAAGCGAAA+TGG | + | chr3.1:56862948-56862967 | None:intergenic | 30.0% | |
| ACATAAACACAAAGCGAAAT+GGG | + | chr3.1:56862947-56862966 | None:intergenic | 30.0% | |
| ATGGGAACAAAAACAAAAAG+CGG | + | chr3.1:56862929-56862948 | None:intergenic | 30.0% | |
| AATGTTGTGCAGATTAAGCT+TGG | - | chr3.1:56862860-56862879 | MS.gene057356:CDS | 35.0% | |
| ATGTTGTGCAGATTAAGCTT+GGG | - | chr3.1:56862861-56862880 | MS.gene057356:CDS | 35.0% | |
| ATTAACATGAGCAACATGAG+AGG | + | chr3.1:56862759-56862778 | None:intergenic | 35.0% | |
| TACATTTGTGATTGCGTCTA+AGG | + | chr3.1:56862528-56862547 | None:intergenic | 35.0% | |
| TCTCATGTTGCTCATGTTAA+TGG | - | chr3.1:56862758-56862777 | MS.gene057356:CDS | 35.0% | |
| AATGGATCTGTGCTAAGAGT+TGG | - | chr3.1:56862776-56862795 | MS.gene057356:CDS | 40.0% | |
| AGTATACATGAAATGCGACG+AGG | + | chr3.1:56862664-56862683 | None:intergenic | 40.0% | |
| ATGGATCTGTGCTAAGAGTT+GGG | - | chr3.1:56862777-56862796 | MS.gene057356:CDS | 40.0% | |
| GGAAGTTCTTGAAAGACGAA+TGG | + | chr3.1:56862738-56862757 | None:intergenic | 40.0% | |
| ! | ATGAAATGCGACGAGGTTTT+CGG | + | chr3.1:56862657-56862676 | None:intergenic | 40.0% |
| !! | TCTGGGATTGGATCTGTTTT+AGG | - | chr3.1:56862809-56862828 | MS.gene057356:CDS | 40.0% |
| AGAGTTGGGATGCTTGTTTC+TGG | - | chr3.1:56862791-56862810 | MS.gene057356:CDS | 45.0% | |
| GAGCGATAAGACTAGAGAAG+AGG | + | chr3.1:56862700-56862719 | None:intergenic | 45.0% | |
| TTGTGGGAGTCAACATGCTT+TGG | - | chr3.1:56862892-56862911 | MS.gene057356:CDS | 45.0% | |
| ! | GAGTTGGGATGCTTGTTTCT+GGG | - | chr3.1:56862792-56862811 | MS.gene057356:CDS | 45.0% |
| ! | TTGCGTCTAAGGCTGTTATG+TGG | + | chr3.1:56862517-56862536 | None:intergenic | 45.0% |
| !! | GCTGGTGATTGATGATGAGA+GGG | + | chr3.1:56862492-56862511 | None:intergenic | 45.0% |
| !! | TCTGTGTTGAGAGTGTTGGA+GGG | + | chr3.1:56862605-56862624 | None:intergenic | 45.0% |
| GTAAACTCCCTCTTCACCCT+CGG | - | chr3.1:56862545-56862564 | MS.gene057356:CDS | 50.0% | |
| TAAGGCTGTTATGTGGACGC+TGG | + | chr3.1:56862510-56862529 | None:intergenic | 50.0% | |
| TTCACCCTCGGCGTATTCAT+CGG | - | chr3.1:56862557-56862576 | MS.gene057356:CDS | 50.0% | |
| ! | AAGCTTGGGACTGTTGCTTG+TGG | - | chr3.1:56862875-56862894 | MS.gene057356:CDS | 50.0% |
| ! | AGCTTGGGACTGTTGCTTGT+GGG | - | chr3.1:56862876-56862895 | MS.gene057356:CDS | 50.0% |
| !! | CGCTGGTGATTGATGATGAG+AGG | + | chr3.1:56862493-56862512 | None:intergenic | 50.0% |
| !! | CTGTGTTGAGAGTGTTGGAG+GGG | + | chr3.1:56862604-56862623 | None:intergenic | 50.0% |
| !! | GGGATGCTTGTTTCTGGGAT+TGG | - | chr3.1:56862797-56862816 | MS.gene057356:CDS | 50.0% |
| !! | GTCTGTGTTGAGAGTGTTGG+AGG | + | chr3.1:56862606-56862625 | None:intergenic | 50.0% |
| GAATACGCCGAGGGTGAAGA+GGG | + | chr3.1:56862555-56862574 | None:intergenic | 55.0% | |
| TGAATACGCCGAGGGTGAAG+AGG | + | chr3.1:56862556-56862575 | None:intergenic | 55.0% | |
| ! | GGGGTCTGTGTTGAGAGTGT+TGG | + | chr3.1:56862609-56862628 | None:intergenic | 55.0% |
| !! | AGAGTGTTGGAGGGGTCGTT+TGG | + | chr3.1:56862596-56862615 | None:intergenic | 55.0% |
| !! | GAGTGTTGGAGGGGTCGTTT+GGG | + | chr3.1:56862595-56862614 | None:intergenic | 55.0% |
| !! | GGTCGTTTGGGTTGAAGGAG+AGG | + | chr3.1:56862583-56862602 | None:intergenic | 55.0% |
| AGAGGCCGATGAATACGCCG+AGG | + | chr3.1:56862565-56862584 | None:intergenic | 60.0% | |
| GAGGCCGATGAATACGCCGA+GGG | + | chr3.1:56862564-56862583 | None:intergenic | 60.0% | |
| ! | GACGAGGTTTTCGGCGATGG+CGG | + | chr3.1:56862648-56862667 | None:intergenic | 60.0% |
| ! | TGCGACGAGGTTTTCGGCGA+TGG | + | chr3.1:56862651-56862670 | None:intergenic | 60.0% |
| !! | GGAGGGGTCGTTTGGGTTGA+AGG | + | chr3.1:56862588-56862607 | None:intergenic | 60.0% |
| !! | TTTTCGGCGATGGCGGTGGT+GGG | + | chr3.1:56862641-56862660 | None:intergenic | 60.0% |
| ! | TTTCGGCGATGGCGGTGGTG+GGG | + | chr3.1:56862640-56862659 | None:intergenic | 65.0% |
| !! | GAGGTTTTCGGCGATGGCGG+TGG | + | chr3.1:56862645-56862664 | None:intergenic | 65.0% |
| !! | GTTTTCGGCGATGGCGGTGG+TGG | + | chr3.1:56862642-56862661 | None:intergenic | 65.0% |
| !! | CGGTGGTGGGGACACAGGAG+GGG | + | chr3.1:56862628-56862647 | None:intergenic | 70.0% |
| !! | GATGGCGGTGGTGGGGACAC+AGG | + | chr3.1:56862633-56862652 | None:intergenic | 70.0% |
| !! | GCGGTGGTGGGGACACAGGA+GGG | + | chr3.1:56862629-56862648 | None:intergenic | 70.0% |
| !! | GGCGGTGGTGGGGACACAGG+AGG | + | chr3.1:56862630-56862649 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 56862485 | 56862997 | 56862485 | ID=MS.gene057356 |
| chr3.1 | mRNA | 56862485 | 56862997 | 56862485 | ID=MS.gene057356.t1;Parent=MS.gene057356 |
| chr3.1 | exon | 56862485 | 56862997 | 56862485 | ID=MS.gene057356.t1.exon1;Parent=MS.gene057356.t1 |
| chr3.1 | CDS | 56862485 | 56862997 | 56862485 | ID=cds.MS.gene057356.t1;Parent=MS.gene057356.t1 |
| Gene Sequence |
| Protein sequence |
