AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69532


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69532.t1 MTR_3g047140 75.986 279 61 3 1 274 1 278 4.35e-145 408
MS.gene69532.t1 MTR_0163s0020 64.982 277 91 2 1 273 2 276 3.44e-111 322
MS.gene69532.t1 MTR_0684s0020 64.982 277 91 2 1 273 1 275 3.55e-111 322
MS.gene69532.t1 MTR_3g054170 53.600 250 111 3 9 257 9 254 3.01e-81 245
MS.gene69532.t1 MTR_3g054180 53.175 252 111 4 9 257 13 260 4.49e-79 240
MS.gene69532.t1 MTR_1g090973 49.810 263 123 5 1 257 1 260 7.71e-77 234
MS.gene69532.t1 MTR_3g054200 50.800 250 118 3 9 257 9 254 5.19e-74 227
MS.gene69532.t1 MTR_1g090943 48.302 265 128 7 10 271 15 273 3.35e-68 213
MS.gene69532.t1 MTR_1g090960 45.000 280 104 8 1 269 1 241 4.26e-62 196
MS.gene69532.t1 MTR_3g027280 47.887 284 124 14 1 267 1 277 6.47e-60 192
MS.gene69532.t1 MTR_1g090937 42.553 282 135 13 1 267 1 270 1.04e-49 165
MS.gene69532.t1 MTR_1g090957 44.643 280 121 15 1 264 1 262 6.56e-49 163
MS.gene69532.t1 MTR_1g090930 40.972 288 139 11 1 269 1 276 3.23e-47 159
MS.gene69532.t1 MTR_4g023810 41.736 242 129 7 26 263 27 260 5.81e-44 150
MS.gene69532.t1 MTR_8g023510 40.755 265 143 10 1 257 1 259 4.44e-42 145
MS.gene69532.t1 MTR_8g068040 38.996 259 136 10 10 255 10 259 1.55e-41 144
MS.gene69532.t1 MTR_8g068030 37.729 273 142 11 1 255 1 263 2.27e-40 141
MS.gene69532.t1 MTR_8g068050 37.736 265 140 8 10 259 21 275 1.66e-37 140
MS.gene69532.t1 MTR_4g093080 34.601 263 154 8 8 258 4 260 8.24e-34 130
MS.gene69532.t1 MTR_5g031160 37.200 250 135 8 26 257 42 287 9.95e-34 124
MS.gene69532.t1 MTR_5g031090 34.413 247 140 8 30 257 32 275 3.29e-33 122
MS.gene69532.t1 MTR_8g067980 37.083 240 128 11 32 255 25 257 1.65e-32 120
MS.gene69532.t1 MTR_5g031120 36.992 246 132 8 31 257 38 279 2.03e-32 120
MS.gene69532.t1 MTR_8g067940 35.849 265 145 10 11 255 20 279 2.21e-32 120
MS.gene69532.t1 MTR_5g031030 34.568 243 141 8 30 257 35 274 4.63e-32 119
MS.gene69532.t1 MTR_5g031140 38.115 244 131 9 31 257 46 286 1.01e-31 119
MS.gene69532.t1 MTR_8g067990 37.344 241 126 12 32 255 25 257 3.71e-31 117
MS.gene69532.t1 MTR_4g056320 35.612 278 128 12 2 270 6 241 2.16e-30 114
MS.gene69532.t1 MTR_5g031100 35.772 246 134 9 31 257 38 278 9.28e-30 113
MS.gene69532.t1 MTR_8g067735 32.103 271 165 9 9 267 25 288 2.23e-29 117
MS.gene69532.t1 MTR_7g115740 33.906 233 125 11 46 258 38 261 5.18e-29 116
MS.gene69532.t1 MTR_4g093110 34.799 273 133 13 10 257 12 264 5.54e-28 113
MS.gene69532.t1 MTR_7g116130 33.712 264 140 13 19 260 14 264 3.20e-27 110
MS.gene69532.t1 MTR_4g093140 33.456 272 138 11 10 257 12 264 5.37e-27 110
MS.gene69532.t1 MTR_4g093040 32.794 247 144 9 11 240 13 254 8.05e-27 109
MS.gene69532.t1 MTR_8g067960 37.168 226 120 10 46 257 24 241 9.13e-26 102
MS.gene69532.t1 MTR_4g093040 39.306 173 88 7 82 240 3 172 2.45e-25 105
MS.gene69532.t1 MTR_1g014240 35.407 209 118 6 71 265 78 283 5.14e-25 104
MS.gene69532.t1 MTR_4g093070 34.328 268 143 9 10 257 12 266 2.40e-23 99.8
MS.gene69532.t1 MTR_8g067930 29.889 271 171 8 9 267 6 269 3.31e-23 99.4
MS.gene69532.t1 MTR_8g067630 33.040 227 131 8 31 243 30 249 3.54e-23 99.0
MS.gene69532.t1 MTR_3g463370 31.734 271 129 9 10 269 9 234 3.89e-23 95.1
MS.gene69532.t1 MTR_8g067720 30.153 262 160 9 11 260 10 260 1.04e-22 97.8
MS.gene69532.t1 MTR_8g067690 29.412 238 154 6 31 257 60 294 2.29e-20 90.9
MS.gene69532.t1 MTR_4g093050 30.980 255 155 10 16 253 12 262 2.95e-19 87.8
MS.gene69532.t1 MTR_5g025020 33.690 187 103 7 72 240 88 271 2.31e-18 85.1
MS.gene69532.t1 MTR_7g063010 30.544 239 127 11 42 254 32 257 3.73e-16 78.6
MS.gene69532.t1 MTR_1g048360 28.458 253 162 6 15 256 17 261 7.45e-16 77.8
MS.gene69532.t1 MTR_5g077100 32.273 220 129 7 50 259 45 254 4.47e-15 75.5
MS.gene69532.t1 MTR_7g063030 30.545 275 153 14 4 254 1 261 4.98e-14 72.0
MS.gene69532.t1 MTR_2g090410 34.694 147 85 6 46 186 34 175 6.48e-14 72.0
MS.gene69532.t1 MTR_7g062940 28.839 267 157 12 9 254 2 256 1.24e-13 70.9
MS.gene69532.t1 MTR_7g115740 32.593 135 70 7 46 167 38 164 1.33e-13 70.9
MS.gene69532.t1 MTR_3g068025 28.315 279 150 12 9 254 7 268 3.12e-13 69.7
MS.gene69532.t1 MTR_2g090250 26.872 227 148 5 44 253 34 259 1.23e-12 68.2
MS.gene69532.t1 MTR_5g035910 28.992 238 135 10 42 254 36 264 7.38e-11 62.8
MS.gene69532.t1 MTR_7g062890 30.244 205 116 9 72 254 58 257 7.90e-11 62.8
MS.gene69532.t1 MTR_8g068050 28.916 166 104 4 10 170 21 177 9.33e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69532.t1 AT5G10530 35.019 257 139 13 19 258 10 255 1.46e-23 100
MS.gene69532.t1 AT5G65600 34.211 190 106 7 71 243 76 263 2.15e-19 88.2
MS.gene69532.t1 AT5G55830 31.716 268 153 11 10 255 7 266 8.69e-18 83.6
MS.gene69532.t1 AT5G03140 29.245 212 135 6 50 251 51 257 9.81e-16 77.4
MS.gene69532.t1 AT4G29050 27.363 201 127 5 72 254 60 259 5.00e-13 69.3
MS.gene69532.t1 AT4G29050 27.363 201 127 5 72 254 74 273 5.22e-13 69.3
MS.gene69532.t1 AT3G55550 29.583 240 142 9 34 253 26 258 1.20e-12 68.2
MS.gene69532.t1 AT1G53080 29.694 229 128 10 59 258 57 281 8.06e-12 64.7
MS.gene69532.t1 AT4G04960 33.775 151 90 4 46 190 35 181 7.72e-11 62.8

Find 53 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACCAATGCTAACTGTTTC+TGG 0.178047 3.1:-33142689 None:intergenic
AGATTTGGAATTACACTATT+TGG 0.215421 3.1:-33142368 None:intergenic
ATTAACCCTTATACGATTAT+AGG 0.243083 3.1:+33142203 MS.gene69532:CDS
CCATCTGTGTTGTTAAGATT+TGG 0.245140 3.1:-33142383 None:intergenic
GCACGACCTATAATCGTATA+AGG 0.280994 3.1:-33142209 None:intergenic
GATCCTAACAATGCACATAT+TGG 0.297717 3.1:+33142488 MS.gene69532:CDS
TTGATAATTATCACAATGAT+TGG 0.305021 3.1:+33142465 MS.gene69532:CDS
TCAAGGGAATGCTGTCATTC+AGG 0.316108 3.1:+33142154 MS.gene69532:CDS
ACAGTTAGCATTGGTCTATC+TGG 0.319313 3.1:+33142695 MS.gene69532:CDS
ATTCAACACCCGTTCCCATT+TGG 0.342088 3.1:+33142237 MS.gene69532:CDS
GACTTAGCTGATTACATCTC+TGG 0.349333 3.1:+33142113 MS.gene69532:CDS
CAACACAGATGGTGGAGATC+TGG 0.350959 3.1:+33142394 MS.gene69532:CDS
GGGAACGGGTGTTGAATATA+AGG 0.357167 3.1:-33142231 None:intergenic
TTCAACACCCGTTCCCATTT+GGG 0.360630 3.1:+33142238 MS.gene69532:CDS
GAAACTCACTTGGAGCTTAC+TGG 0.370616 3.1:+33142176 MS.gene69532:CDS
CCCCATGGGATTCACAAAAC+GGG 0.386898 3.1:+33142546 MS.gene69532:CDS
ACTGCTTTCAATCGATTTGT+TGG 0.405940 3.1:+33142434 MS.gene69532:CDS
TGAAGATGACTAACAGAAAC+TGG 0.410445 3.1:-33142031 None:intergenic
ATCCTAACAATGCACATATT+GGG 0.411998 3.1:+33142489 MS.gene69532:CDS
CCCATTTGGGATAGTGCAAC+TGG 0.412436 3.1:+33142251 MS.gene69532:CDS
GCCAGTTGCACTATCCCAAA+TGG 0.414802 3.1:-33142252 None:intergenic
ACTAACAGAAACTGGTTAGT+TGG 0.419127 3.1:-33142023 None:intergenic
GAATGACAGCATTCCCTTGA+AGG 0.432002 3.1:-33142151 None:intergenic
TCCCCATGGGATTCACAAAA+CGG 0.443828 3.1:+33142545 MS.gene69532:CDS
TGATAATTATCACAATGATT+GGG 0.445401 3.1:+33142466 MS.gene69532:CDS
ATCCCAATATGTGCATTGTT+AGG 0.450564 3.1:-33142491 None:intergenic
GACCAATGCTAACTGTTTCT+GGG 0.454186 3.1:-33142688 None:intergenic
TGAATCCCATGGGGATGTCT+TGG 0.462545 3.1:-33142537 None:intergenic
GTTTCTCTCATTGACGAGAA+TGG 0.472278 3.1:+33142620 MS.gene69532:CDS
TTCAGTTACTAGCAAGAGAA+AGG 0.481258 3.1:-33142060 None:intergenic
GCACTATCCCAAATGGGAAC+GGG 0.488710 3.1:-33142245 None:intergenic
AACACAGATGGTGGAGATCT+GGG 0.502642 3.1:+33142395 MS.gene69532:CDS
CACGACCTATAATCGTATAA+GGG 0.505615 3.1:-33142208 None:intergenic
TTTGGAATTACACTATTTGG+TGG 0.521911 3.1:-33142365 None:intergenic
ACTTAGCTGATTACATCTCT+GGG 0.525752 3.1:+33142114 MS.gene69532:CDS
CCAGTTGCACTATCCCAAAT+GGG 0.537384 3.1:-33142251 None:intergenic
TGCACTATCCCAAATGGGAA+CGG 0.540036 3.1:-33142246 None:intergenic
AATGACAGCATTCCCTTGAA+GGG 0.551329 3.1:-33142150 None:intergenic
GGTAGTTATCCACGAGCTGA+TGG 0.555952 3.1:+33142323 MS.gene69532:CDS
CTCCCAGAAACAGTTAGCAT+TGG 0.565328 3.1:+33142686 MS.gene69532:CDS
CCAAATCTTAACAACACAGA+TGG 0.577172 3.1:+33142383 MS.gene69532:CDS
AATCTTAACAACACAGATGG+TGG 0.587455 3.1:+33142386 MS.gene69532:CDS
TGTCATTCAGGAAACTCACT+TGG 0.593945 3.1:+33142166 MS.gene69532:CDS
CAAATCAACGACTTGAGCAA+CGG 0.597604 3.1:-33142654 None:intergenic
TTCATCCAAGACATCCCCAT+GGG 0.607724 3.1:+33142532 MS.gene69532:CDS
AAGATAAGACCATCAGCTCG+TGG 0.612801 3.1:-33142332 None:intergenic
GATGTAATCAGCTAAGTCGA+AGG 0.627754 3.1:-33142108 None:intergenic
AAATATATGTCATTGAAACA+TGG 0.630792 3.1:+33142741 MS.gene69532:CDS
CCCATGGGATTCACAAAACG+GGG 0.631871 3.1:+33142547 MS.gene69532:CDS
AGTATGATTTATGTTCTCTG+AGG 0.647177 3.1:-33142804 None:intergenic
TTTCATCCAAGACATCCCCA+TGG 0.656398 3.1:+33142531 MS.gene69532:CDS
GCTCGTGGATAACTACCGAA+CGG 0.706851 3.1:-33142317 None:intergenic
CTTAGCTGATTACATCTCTG+GGG 0.723307 3.1:+33142115 MS.gene69532:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATGTCATTGAAACA+TGG + chr3.1:33142741-33142760 MS.gene69532:CDS 20.0%
!! TGATAATTATCACAATGATT+GGG + chr3.1:33142466-33142485 MS.gene69532:CDS 20.0%
!!! TTGATAATTATCACAATGAT+TGG + chr3.1:33142465-33142484 MS.gene69532:CDS 20.0%
! AAAAAACTTTAACCCTTCAA+GGG + chr3.1:33142138-33142157 MS.gene69532:CDS 25.0%
! ATTAACCCTTATACGATTAT+AGG + chr3.1:33142203-33142222 MS.gene69532:CDS 25.0%
!! AGATTTGGAATTACACTATT+TGG - chr3.1:33142371-33142390 None:intergenic 25.0%
AGTATGATTTATGTTCTCTG+AGG - chr3.1:33142807-33142826 None:intergenic 30.0%
ATCCTAACAATGCACATATT+GGG + chr3.1:33142489-33142508 MS.gene69532:CDS 30.0%
GAAAAAACTTTAACCCTTCA+AGG + chr3.1:33142137-33142156 MS.gene69532:CDS 30.0%
! TTTGGAATTACACTATTTGG+TGG - chr3.1:33142368-33142387 None:intergenic 30.0%
AATCTTAACAACACAGATGG+TGG + chr3.1:33142386-33142405 MS.gene69532:CDS 35.0%
ACTAACAGAAACTGGTTAGT+TGG - chr3.1:33142026-33142045 None:intergenic 35.0%
ACTTAGCTGATTACATCTCT+GGG + chr3.1:33142114-33142133 MS.gene69532:CDS 35.0%
ATCCCAATATGTGCATTGTT+AGG - chr3.1:33142494-33142513 None:intergenic 35.0%
CACGACCTATAATCGTATAA+GGG - chr3.1:33142211-33142230 None:intergenic 35.0%
CCAAATCTTAACAACACAGA+TGG + chr3.1:33142383-33142402 MS.gene69532:CDS 35.0%
GATCCTAACAATGCACATAT+TGG + chr3.1:33142488-33142507 MS.gene69532:CDS 35.0%
TGAAGATGACTAACAGAAAC+TGG - chr3.1:33142034-33142053 None:intergenic 35.0%
TTCAGTTACTAGCAAGAGAA+AGG - chr3.1:33142063-33142082 None:intergenic 35.0%
! CCATCTGTGTTGTTAAGATT+TGG - chr3.1:33142386-33142405 None:intergenic 35.0%
!! ACTGCTTTCAATCGATTTGT+TGG + chr3.1:33142434-33142453 MS.gene69532:CDS 35.0%
AATGACAGCATTCCCTTGAA+GGG - chr3.1:33142153-33142172 None:intergenic 40.0%
AGACCAATGCTAACTGTTTC+TGG - chr3.1:33142692-33142711 None:intergenic 40.0%
CAAATCAACGACTTGAGCAA+CGG - chr3.1:33142657-33142676 None:intergenic 40.0%
CTTAGCTGATTACATCTCTG+GGG + chr3.1:33142115-33142134 MS.gene69532:CDS 40.0%
GACTTAGCTGATTACATCTC+TGG + chr3.1:33142113-33142132 MS.gene69532:CDS 40.0%
GATGTAATCAGCTAAGTCGA+AGG - chr3.1:33142111-33142130 None:intergenic 40.0%
GCACGACCTATAATCGTATA+AGG - chr3.1:33142212-33142231 None:intergenic 40.0%
GTTTCTCTCATTGACGAGAA+TGG + chr3.1:33142620-33142639 MS.gene69532:CDS 40.0%
TGTCATTCAGGAAACTCACT+TGG + chr3.1:33142166-33142185 MS.gene69532:CDS 40.0%
! GACCAATGCTAACTGTTTCT+GGG - chr3.1:33142691-33142710 None:intergenic 40.0%
!! ACAGTTAGCATTGGTCTATC+TGG + chr3.1:33142695-33142714 MS.gene69532:CDS 40.0%
!! TCTTTTTCTCTTGTGCCGTT+CGG + chr3.1:33142302-33142321 MS.gene69532:CDS 40.0%
!! TTGACGTTTTTCAACTGACG+TGG - chr3.1:33142723-33142742 None:intergenic 40.0%
AAGATAAGACCATCAGCTCG+TGG - chr3.1:33142335-33142354 None:intergenic 45.0%
ATTCAACACCCGTTCCCATT+TGG + chr3.1:33142237-33142256 MS.gene69532:CDS 45.0%
CCAGTTGCACTATCCCAAAT+GGG - chr3.1:33142254-33142273 None:intergenic 45.0%
CGGCACAAGAGAAAAAGAGA+AGG - chr3.1:33142300-33142319 None:intergenic 45.0%
CTCCCAGAAACAGTTAGCAT+TGG + chr3.1:33142686-33142705 MS.gene69532:CDS 45.0%
GAAACTCACTTGGAGCTTAC+TGG + chr3.1:33142176-33142195 MS.gene69532:CDS 45.0%
GAATGACAGCATTCCCTTGA+AGG - chr3.1:33142154-33142173 None:intergenic 45.0%
TCAAGGGAATGCTGTCATTC+AGG + chr3.1:33142154-33142173 MS.gene69532:CDS 45.0%
TCCCCATGGGATTCACAAAA+CGG + chr3.1:33142545-33142564 MS.gene69532:CDS 45.0%
TGCACTATCCCAAATGGGAA+CGG - chr3.1:33142249-33142268 None:intergenic 45.0%
TTCAACACCCGTTCCCATTT+GGG + chr3.1:33142238-33142257 MS.gene69532:CDS 45.0%
TTCATCCAAGACATCCCCAT+GGG + chr3.1:33142532-33142551 MS.gene69532:CDS 45.0%
TTTCATCCAAGACATCCCCA+TGG + chr3.1:33142531-33142550 MS.gene69532:CDS 45.0%
!! AACACAGATGGTGGAGATCT+GGG + chr3.1:33142395-33142414 MS.gene69532:CDS 45.0%
!! GGGAACGGGTGTTGAATATA+AGG - chr3.1:33142234-33142253 None:intergenic 45.0%
CCCATGGGATTCACAAAACG+GGG + chr3.1:33142547-33142566 MS.gene69532:CDS 50.0%
CCCCATGGGATTCACAAAAC+GGG + chr3.1:33142546-33142565 MS.gene69532:CDS 50.0%
GCACTATCCCAAATGGGAAC+GGG - chr3.1:33142248-33142267 None:intergenic 50.0%
GCCAGTTGCACTATCCCAAA+TGG - chr3.1:33142255-33142274 None:intergenic 50.0%
GCTCGTGGATAACTACCGAA+CGG - chr3.1:33142320-33142339 None:intergenic 50.0%
GGTAGTTATCCACGAGCTGA+TGG + chr3.1:33142323-33142342 MS.gene69532:CDS 50.0%
TGAATCCCATGGGGATGTCT+TGG - chr3.1:33142540-33142559 None:intergenic 50.0%
! ACCCCGTTTTGTGAATCCCA+TGG - chr3.1:33142551-33142570 None:intergenic 50.0%
! CCCATTTGGGATAGTGCAAC+TGG + chr3.1:33142251-33142270 MS.gene69532:CDS 50.0%
! CCCCGTTTTGTGAATCCCAT+GGG - chr3.1:33142550-33142569 None:intergenic 50.0%
! CCCGTTTTGTGAATCCCATG+GGG - chr3.1:33142549-33142568 None:intergenic 50.0%
!! CAACACAGATGGTGGAGATC+TGG + chr3.1:33142394-33142413 MS.gene69532:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 33142011 33142835 33142011 ID=MS.gene69532
chr3.1 mRNA 33142011 33142835 33142011 ID=MS.gene69532.t1;Parent=MS.gene69532
chr3.1 exon 33142011 33142835 33142011 ID=MS.gene69532.t1.exon1;Parent=MS.gene69532.t1
chr3.1 CDS 33142011 33142835 33142011 ID=cds.MS.gene69532.t1;Parent=MS.gene69532.t1
Gene Sequence

>MS.gene69532

ATGGCTTTCTTTCCAACTAACCAGTTTCTGTTAGTCATCTTCACAACTTCCTTTCTCTTGCTAGTAACTGAATTTGTGAACTCAGACGAAACAGTTTCCTTCGACTTAGCTGATTACATCTCTGGGGAAAAAACTTTAACCCTTCAAGGGAATGCTGTCATTCAGGAAACTCACTTGGAGCTTACTGGTGACATTAACCCTTATACGATTATAGGTCGTGCCTTATATTCAACACCCGTTCCCATTTGGGATAGTGCAACTGGCAATGTCGCTAGTTTTGTGACTTCCTTCTCTTTTTCTCTTGTGCCGTTCGGTAGTTATCCACGAGCTGATGGTCTTATCTTCTTTCTTGCACCACCAAATAGTGTAATTCCAAATCTTAACAACACAGATGGTGGAGATCTGGGAGTAGTCGATGAAACAACTGCTTTCAATCGATTTGTTGGTGTAGAATTTGATAATTATCACAATGATTGGGATCCTAACAATGCACATATTGGGATCGATGTCAACTCTTTAATTTCATCCAAGACATCCCCATGGGATTCACAAAACGGGGTGTTGTACAATGTTAGTATCATCTATGATTCTTCTTCTAAGATTTTGAGTGTTTCTCTCATTGACGAGAATGGTCAACTTTCTACCGTTGCTCAAGTCGTTGATTTGAAAGCTGTGCTCCCAGAAACAGTTAGCATTGGTCTATCTGGTTCCACGTCAGTTGAAAAACGTCAAATATATGTCATTGAAACATGGTCTTTCAAGTCAATTTTGAAGACAACTATAAGCAGCAGCACCTCAGAGAACATAAATCATACTGCAATCTAG

Protein sequence

>MS.gene69532.t1

MAFFPTNQFLLVIFTTSFLLLVTEFVNSDETVSFDLADYISGEKTLTLQGNAVIQETHLELTGDINPYTIIGRALYSTPVPIWDSATGNVASFVTSFSFSLVPFGSYPRADGLIFFLAPPNSVIPNLNNTDGGDLGVVDETTAFNRFVGVEFDNYHNDWDPNNAHIGIDVNSLISSKTSPWDSQNGVLYNVSIIYDSSSKILSVSLIDENGQLSTVAQVVDLKAVLPETVSIGLSGSTSVEKRQIYVIETWSFKSILKTTISSSTSENINHTAI