AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69596

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69596.t1 MTR_8g043650 68.776 237 26 1 1 189 1 237 7.85e-113 321
MS.gene69596.t1 MTR_3g052920 72.018 218 13 1 1 170 1 218 4.10e-108 309
MS.gene69596.t1 MTR_2g030740 70.886 237 21 1 1 189 1 237 1.54e-104 300
MS.gene69596.t1 MTR_1g105910 67.511 237 29 1 1 189 1 237 4.09e-102 294
MS.gene69596.t1 MTR_1g105920 67.890 218 22 1 1 170 1 218 1.49e-101 293
MS.gene69596.t1 MTR_1g012570 67.932 237 28 1 1 189 1 237 4.83e-99 286
MS.gene69596.t1 MTR_1g105905 61.818 220 36 1 18 189 1 220 1.38e-91 266
MS.gene69596.t1 MTR_5g055100 52.321 237 65 1 1 189 1 237 1.18e-81 242
MS.gene69596.t1 MTR_3g052870 62.719 228 18 2 1 180 1 209 2.29e-81 241
MS.gene69596.t1 MTR_0121s0080 55.274 237 57 2 1 189 1 236 7.83e-80 238
MS.gene69596.t1 MTR_2g085280 51.271 236 67 1 1 188 1 236 1.05e-79 237
MS.gene69596.t1 MTR_2g085250 51.271 236 67 1 1 188 1 236 3.46e-79 236
MS.gene69596.t1 MTR_1g077360 54.667 225 54 1 1 177 1 225 4.51e-78 233
MS.gene69596.t1 MTR_1g075600 54.222 225 55 1 1 177 1 225 1.41e-76 229
MS.gene69596.t1 MTR_5g053390 53.778 225 56 1 1 177 1 225 3.37e-76 228
MS.gene69596.t1 MTR_1g108510 55.319 235 39 4 1 177 1 227 5.53e-76 227
MS.gene69596.t1 MTR_3g030770 54.468 235 41 4 1 177 1 227 3.25e-75 225
MS.gene69596.t1 MTR_5g045560 53.165 237 63 1 1 189 1 237 2.63e-74 223
MS.gene69596.t1 MTR_1g106070 62.088 182 21 1 1 134 1 182 1.84e-72 217
MS.gene69596.t1 MTR_2g093190 53.617 235 43 4 1 177 1 227 3.38e-71 215
MS.gene69596.t1 MTR_1g108580 52.766 235 45 4 1 177 1 227 1.57e-70 214
MS.gene69596.t1 MTR_7g062350 48.729 236 71 3 3 189 14 248 1.42e-65 202
MS.gene69596.t1 MTR_1g075570 56.098 164 38 1 18 147 1 164 9.13e-48 154
MS.gene69596.t1 MTR_3g030780 83.607 61 10 0 1 61 1 61 4.58e-30 107
MS.gene69596.t1 MTR_0121s0100 80.328 61 12 0 1 61 1 61 1.99e-28 103
MS.gene69596.t1 MTR_3g080940 38.312 154 47 2 7 112 7 160 3.14e-28 105
MS.gene69596.t1 MTR_2g105290 33.735 166 66 1 5 126 8 173 1.13e-24 95.1
MS.gene69596.t1 MTR_8g086290 33.133 166 63 2 5 122 8 173 1.28e-23 92.4
MS.gene69596.t1 MTR_3g067875 79.545 44 9 0 18 61 1 44 3.25e-19 77.8
MS.gene69596.t1 MTR_7g028448 38.776 147 46 3 5 111 8 150 7.19e-18 77.0
MS.gene69596.t1 MTR_4g036915 38.776 147 46 3 5 111 8 150 7.19e-18 77.0
MS.gene69596.t1 MTR_1g047550 63.462 52 19 0 9 60 2 53 1.65e-16 73.9
MS.gene69596.t1 MTR_1g108500 67.857 56 16 1 8 61 94 149 1.99e-16 76.3
MS.gene69596.t1 MTR_8g079502 63.462 52 19 0 9 60 2 53 3.58e-16 73.2
MS.gene69596.t1 MTR_4g127140 31.638 177 67 3 5 127 8 184 1.87e-15 71.2
MS.gene69596.t1 MTR_1g054265 62.000 50 19 0 9 58 2 51 2.12e-15 70.9
MS.gene69596.t1 MTR_8g051580 61.538 52 20 0 9 60 2 53 4.54e-15 70.1
MS.gene69596.t1 MTR_3g067870 67.391 46 15 0 16 61 83 128 5.53e-15 69.3
MS.gene69596.t1 MTR_1g063160 60.000 50 20 0 9 58 2 51 7.13e-15 69.3
MS.gene69596.t1 MTR_4g093970 63.462 52 19 0 10 61 29 80 7.45e-15 70.5
MS.gene69596.t1 MTR_4g131030 59.615 52 21 0 9 60 2 53 8.00e-15 70.1
MS.gene69596.t1 MTR_3g102570 33.566 143 73 3 9 129 2 144 2.83e-14 70.1
MS.gene69596.t1 MTR_2g461710 54.717 53 24 0 9 61 2 54 3.56e-14 64.7
MS.gene69596.t1 MTR_3g452380 60.000 55 22 0 3 57 11 65 4.58e-14 68.6
MS.gene69596.t1 MTR_4g084740 47.761 67 33 1 9 75 2 66 5.67e-14 69.3
MS.gene69596.t1 MTR_1g038300 56.364 55 24 0 9 63 2 56 1.01e-13 67.4
MS.gene69596.t1 MTR_5g032520 47.143 70 37 0 9 78 2 71 1.64e-13 66.6
MS.gene69596.t1 MTR_5g032150 47.143 70 37 0 9 78 2 71 2.10e-13 66.6
MS.gene69596.t1 MTR_3g005530 61.224 49 19 0 9 57 2 50 2.84e-13 65.9
MS.gene69596.t1 MTR_3g067910 70.732 41 12 0 21 61 2 42 3.13e-13 63.5
MS.gene69596.t1 MTR_2g017865 61.224 49 19 0 9 57 17 65 3.40e-13 66.2
MS.gene69596.t1 MTR_8g087860 62.500 48 18 0 9 56 18 65 4.16e-13 65.9
MS.gene69596.t1 MTR_8g046350 57.692 52 22 0 9 60 2 53 4.65e-13 64.3
MS.gene69596.t1 MTR_5g046790 52.830 53 25 0 9 61 2 54 4.90e-13 65.1
MS.gene69596.t1 MTR_4g102530 61.538 52 20 0 10 61 3 54 5.18e-13 65.1
MS.gene69596.t1 MTR_4g108720 34.868 152 78 5 9 160 2 132 8.46e-13 65.9
MS.gene69596.t1 MTR_1g053070 60.417 48 19 0 9 56 2 49 8.71e-13 65.1
MS.gene69596.t1 MTR_8g033220 33.562 146 72 5 10 131 3 147 9.26e-13 64.7
MS.gene69596.t1 MTR_8g033220 33.562 146 72 5 10 131 3 147 9.30e-13 64.7
MS.gene69596.t1 MTR_7g075850 50.000 56 28 0 9 64 2 57 1.03e-12 64.7
MS.gene69596.t1 MTR_5g066960 49.057 53 27 0 9 61 2 54 1.07e-12 60.8
MS.gene69596.t1 MTR_6g464720 52.727 55 26 0 9 63 2 56 1.14e-12 64.7
MS.gene69596.t1 MTR_5g066180 56.604 53 23 0 9 61 2 54 1.17e-12 64.3
MS.gene69596.t1 MTR_8g033270 54.717 53 24 0 9 61 2 54 1.43e-12 64.7
MS.gene69596.t1 MTR_5g021270 50.877 57 28 0 9 65 2 58 1.47e-12 64.3
MS.gene69596.t1 MTR_5g021270 50.877 57 28 0 9 65 2 58 1.55e-12 64.3
MS.gene69596.t1 MTR_7g016600 52.830 53 25 0 9 61 2 54 1.96e-12 63.9
MS.gene69596.t1 MTR_5g046870 48.276 58 30 0 9 66 2 59 2.01e-12 61.6
MS.gene69596.t1 MTR_3g084980 58.333 48 20 0 9 56 2 49 2.28e-12 63.9
MS.gene69596.t1 MTR_4g109830 50.943 53 26 0 9 61 2 54 2.95e-12 63.5
MS.gene69596.t1 MTR_3g113030 47.368 57 30 0 9 65 2 58 3.15e-12 63.2
MS.gene69596.t1 MTR_0003s0590 58.333 48 20 0 9 56 2 49 3.40e-12 63.5
MS.gene69596.t1 MTR_1g029670 35.802 81 52 0 9 89 2 82 3.44e-12 62.4
MS.gene69596.t1 MTR_8g066260 52.830 53 25 0 9 61 2 54 3.61e-12 63.2
MS.gene69596.t1 MTR_7g075870 55.769 52 23 0 10 61 3 54 3.91e-12 62.8
MS.gene69596.t1 MTR_7g075870 55.769 52 23 0 10 61 3 54 3.93e-12 62.8
MS.gene69596.t1 MTR_8g097090 56.250 48 21 0 9 56 2 49 3.95e-12 63.2
MS.gene69596.t1 MTR_6g015975 56.250 48 21 0 9 56 2 49 3.96e-12 63.2
MS.gene69596.t1 MTR_4g109810 54.167 48 22 0 9 56 2 49 4.37e-12 63.2
MS.gene69596.t1 MTR_4g094632 55.769 52 23 0 9 60 2 53 6.66e-12 61.2
MS.gene69596.t1 MTR_3g088615 45.614 57 31 0 9 65 2 58 7.32e-12 61.6
MS.gene69596.t1 MTR_1g101970 57.143 49 21 0 9 57 2 50 1.01e-11 61.2
MS.gene69596.t1 MTR_2g009890 39.773 88 44 2 9 87 2 89 1.96e-11 61.6
MS.gene69596.t1 MTR_4g094638 57.143 49 21 0 9 57 2 50 3.38e-11 59.7
MS.gene69596.t1 MTR_5g031000 39.080 87 45 1 9 87 2 88 8.40e-11 59.3
MS.gene69596.t1 MTR_7g016630 49.057 53 27 0 9 61 2 54 9.65e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69596.t1 AT5G60440 40.426 188 57 4 5 141 3 186 8.00e-34 122
MS.gene69596.t1 AT1G01530 35.638 188 69 4 4 144 2 184 7.19e-26 100
MS.gene69596.t1 AT4G36590 77.193 57 13 0 5 61 3 59 2.74e-24 95.9
MS.gene69596.t1 AT2G24840 36.709 158 51 3 6 114 60 217 1.08e-23 94.7
MS.gene69596.t1 AT1G65360 33.155 187 74 4 5 144 3 185 1.13e-22 91.3
MS.gene69596.t1 AT1G72350 34.454 119 63 2 9 112 43 161 2.35e-16 74.3
MS.gene69596.t1 AT3G66656 65.385 52 18 0 9 60 2 53 6.22e-16 72.4
MS.gene69596.t1 AT2G34440 63.462 52 19 0 9 60 2 53 2.91e-15 70.5
MS.gene69596.t1 AT3G04100 52.727 55 26 0 2 56 7 61 1.49e-14 69.3
MS.gene69596.t1 AT3G54340 34.454 119 74 2 9 124 2 119 4.41e-14 68.6
MS.gene69596.t1 AT1G22130 53.704 54 25 0 9 62 2 55 4.86e-14 69.3
MS.gene69596.t1 AT1G77980 53.704 54 25 0 9 62 2 55 6.11e-14 69.3
MS.gene69596.t1 AT2G22540 56.604 53 23 0 9 61 2 54 8.81e-14 67.8
MS.gene69596.t1 AT2G22540 56.604 53 23 0 9 61 2 54 1.16e-13 67.4
MS.gene69596.t1 AT2G22540 56.604 53 23 0 9 61 2 54 1.16e-13 67.4
MS.gene69596.t1 AT4G09960 61.224 49 19 0 9 57 2 50 1.51e-13 66.6
MS.gene69596.t1 AT4G22950 59.615 52 21 0 10 61 3 54 1.56e-13 65.9
MS.gene69596.t1 AT4G09960 61.224 49 19 0 9 57 2 50 1.63e-13 67.0
MS.gene69596.t1 AT4G18960 63.462 52 18 1 5 56 15 65 1.68e-13 67.0
MS.gene69596.t1 AT4G09960 61.224 49 19 0 9 57 3 51 1.76e-13 66.6
MS.gene69596.t1 AT4G09960 61.224 49 19 0 9 57 12 60 2.13e-13 66.6
MS.gene69596.t1 AT5G23260 53.125 64 28 1 2 63 9 72 2.24e-13 67.0
MS.gene69596.t1 AT5G23260 53.125 64 28 1 2 63 9 72 2.53e-13 67.0
MS.gene69596.t1 AT4G09960 61.224 49 19 0 9 57 28 76 2.74e-13 66.6
MS.gene69596.t1 AT3G58780 60.784 51 20 0 6 56 14 64 2.92e-13 66.2
MS.gene69596.t1 AT3G58780 60.784 51 20 0 6 56 14 64 3.23e-13 66.2
MS.gene69596.t1 AT5G23260 56.364 55 24 0 9 63 2 56 3.45e-13 66.2
MS.gene69596.t1 AT2G42830 54.386 57 26 0 1 57 9 65 3.46e-13 66.2
MS.gene69596.t1 AT2G42830 54.386 57 26 0 1 57 9 65 3.50e-13 66.2
MS.gene69596.t1 AT3G58780 60.784 51 20 0 6 56 20 70 3.55e-13 66.2
MS.gene69596.t1 AT4G22950 59.615 52 21 0 10 61 3 54 3.67e-13 65.9
MS.gene69596.t1 AT4G22950 59.615 52 21 0 10 61 3 54 3.67e-13 65.9
MS.gene69596.t1 AT3G58780 60.784 51 20 0 6 56 14 64 3.90e-13 66.2
MS.gene69596.t1 AT4G24540 56.604 53 23 0 9 61 2 54 4.16e-13 65.5
MS.gene69596.t1 AT5G23260 56.364 55 24 0 9 63 2 56 4.17e-13 66.2
MS.gene69596.t1 AT1G26310 58.333 48 20 0 9 56 2 49 4.71e-13 65.9
MS.gene69596.t1 AT3G58780 60.784 51 20 0 6 56 14 64 5.00e-13 66.2
MS.gene69596.t1 AT3G57390 58.491 53 22 0 9 61 2 54 6.07e-13 65.1
MS.gene69596.t1 AT5G13790 56.604 53 23 0 9 61 2 54 6.70e-13 65.9
MS.gene69596.t1 AT5G13790 56.604 53 23 0 9 61 2 54 7.14e-13 65.5
MS.gene69596.t1 AT4G11880 57.692 52 22 0 10 61 3 54 7.32e-13 64.3
MS.gene69596.t1 AT4G11880 57.692 52 22 0 10 61 3 54 7.32e-13 64.3
MS.gene69596.t1 AT4G09960 61.224 49 19 0 9 57 97 145 7.45e-13 65.9
MS.gene69596.t1 AT2G45650 56.604 53 23 0 9 61 2 54 8.18e-13 65.1
MS.gene69596.t1 AT5G51870 55.556 54 24 0 10 63 3 56 9.97e-13 63.5
MS.gene69596.t1 AT1G77950 50.943 53 26 0 9 61 2 54 1.04e-12 65.1
MS.gene69596.t1 AT1G77950 50.943 53 26 0 9 61 2 54 1.04e-12 65.1
MS.gene69596.t1 AT1G77950 50.943 53 26 0 9 61 2 54 1.04e-12 65.1
MS.gene69596.t1 AT1G77950 50.943 53 26 0 9 61 2 54 1.04e-12 65.1
MS.gene69596.t1 AT3G30260 52.830 53 25 0 9 61 2 54 1.05e-12 65.1
MS.gene69596.t1 AT3G57390 58.491 53 22 0 9 61 2 54 1.17e-12 64.7
MS.gene69596.t1 AT4G11880 57.692 52 22 0 10 61 3 54 1.36e-12 64.3
MS.gene69596.t1 AT4G11880 57.692 52 22 0 10 61 3 54 1.36e-12 64.3
MS.gene69596.t1 AT1G69120 58.333 48 20 0 9 56 2 49 1.53e-12 64.3
MS.gene69596.t1 AT5G51870 55.556 54 24 0 10 63 3 56 1.77e-12 63.5
MS.gene69596.t1 AT5G10140 46.269 67 27 1 9 66 2 68 1.82e-12 62.8
MS.gene69596.t1 AT2G03710 56.250 48 21 0 9 56 2 49 1.93e-12 63.2
MS.gene69596.t1 AT5G51870 50.847 59 29 0 5 63 21 79 2.02e-12 63.2
MS.gene69596.t1 AT5G10140 46.269 67 27 1 9 66 2 68 2.49e-12 62.8
MS.gene69596.t1 AT5G10140 43.750 80 35 2 9 78 2 81 2.59e-12 63.2
MS.gene69596.t1 AT3G61120 47.458 59 31 0 9 67 2 60 2.67e-12 63.5
MS.gene69596.t1 AT1G69120 58.333 48 20 0 9 56 2 49 2.83e-12 63.9
MS.gene69596.t1 AT1G24260 56.250 48 21 0 9 56 2 49 2.87e-12 63.5
MS.gene69596.t1 AT5G10140 43.750 80 35 2 9 78 2 81 2.95e-12 62.8
MS.gene69596.t1 AT5G60910 52.830 53 25 0 9 61 2 54 3.16e-12 63.5
MS.gene69596.t1 AT5G62165 59.574 47 19 0 10 56 3 49 3.19e-12 60.8
MS.gene69596.t1 AT1G24260 56.250 48 21 0 9 56 2 49 3.33e-12 63.5
MS.gene69596.t1 AT1G24260 56.250 48 21 0 9 56 2 49 3.57e-12 63.5
MS.gene69596.t1 AT5G51870 55.556 54 24 0 10 63 3 56 3.61e-12 63.2
MS.gene69596.t1 AT5G15800 56.250 48 21 0 9 56 2 49 3.79e-12 63.5
MS.gene69596.t1 AT2G03710 56.250 48 21 0 9 56 2 49 3.97e-12 63.5
MS.gene69596.t1 AT5G51870 55.556 54 24 0 10 63 13 66 4.00e-12 63.2
MS.gene69596.t1 AT2G03710 56.250 48 21 0 9 56 2 49 4.09e-12 63.5
MS.gene69596.t1 AT5G62165 58.333 48 20 0 10 57 3 50 4.75e-12 61.6
MS.gene69596.t1 AT5G62165 58.333 48 20 0 10 57 3 50 4.75e-12 61.6
MS.gene69596.t1 AT5G62165 58.333 48 20 0 10 57 3 50 4.75e-12 61.6
MS.gene69596.t1 AT5G62165 58.333 48 20 0 10 57 3 50 4.75e-12 61.6
MS.gene69596.t1 AT5G62165 58.333 48 20 0 10 57 3 50 4.75e-12 61.6
MS.gene69596.t1 AT3G02310 56.250 48 21 0 9 56 2 49 5.85e-12 62.0
MS.gene69596.t1 AT5G15800 56.250 48 21 0 9 56 2 49 5.94e-12 63.2
MS.gene69596.t1 AT5G62165 58.333 48 20 0 10 57 3 50 5.99e-12 61.2
MS.gene69596.t1 AT5G62165 58.333 48 20 0 10 57 3 50 5.99e-12 61.2
MS.gene69596.t1 AT5G62165 58.333 48 20 0 10 57 3 50 5.99e-12 61.2
MS.gene69596.t1 AT5G20240 52.830 53 25 0 9 61 2 54 7.00e-12 60.8
MS.gene69596.t1 AT3G02310 56.250 48 21 0 9 56 2 49 7.94e-12 62.4
MS.gene69596.t1 AT2G45660 53.846 52 24 0 10 61 3 54 9.72e-12 60.8
MS.gene69596.t1 AT3G57230 53.061 49 23 0 9 57 2 50 1.08e-11 60.5
MS.gene69596.t1 AT5G20240 52.830 53 25 0 9 61 2 54 1.16e-11 61.6
MS.gene69596.t1 AT5G51860 61.702 47 18 0 10 56 3 49 1.49e-11 61.2
MS.gene69596.t1 AT5G62165 59.574 47 19 0 10 56 3 49 1.51e-11 60.8
MS.gene69596.t1 AT5G62165 59.574 47 19 0 10 56 3 49 1.51e-11 60.8
MS.gene69596.t1 AT5G62165 59.574 47 19 0 10 56 3 49 1.68e-11 61.2
MS.gene69596.t1 AT5G62165 59.574 47 19 0 10 56 3 49 1.68e-11 61.2
MS.gene69596.t1 AT5G62165 59.574 47 19 0 10 56 3 49 1.68e-11 61.2
MS.gene69596.t1 AT1G71692 50.000 56 28 0 9 64 2 57 1.98e-11 60.8
MS.gene69596.t1 AT2G45660 53.846 52 24 0 10 61 3 54 2.09e-11 60.8
MS.gene69596.t1 AT5G51860 61.702 47 18 0 10 56 3 49 2.10e-11 60.8
MS.gene69596.t1 AT3G57230 53.061 49 23 0 9 57 2 50 2.39e-11 61.2
MS.gene69596.t1 AT3G57230 53.061 49 23 0 9 57 2 50 2.39e-11 61.2
MS.gene69596.t1 AT2G22630 50.943 53 26 0 9 61 2 54 3.20e-11 60.5
MS.gene69596.t1 AT2G22630 50.943 53 26 0 9 61 2 54 3.20e-11 60.5
MS.gene69596.t1 AT4G37940 37.500 88 46 2 9 87 2 89 3.24e-11 59.7
MS.gene69596.t1 AT2G14210 39.326 89 44 3 9 87 2 90 3.42e-11 60.5
MS.gene69596.t1 AT5G65050 32.258 124 67 5 9 122 2 118 3.72e-11 59.7
MS.gene69596.t1 AT2G14210 39.326 89 44 3 9 87 2 90 3.97e-11 60.5
MS.gene69596.t1 AT4G37940 37.500 88 46 2 9 87 2 89 5.35e-11 59.7
MS.gene69596.t1 AT5G65050 32.258 124 67 5 9 122 2 118 5.79e-11 59.3
MS.gene69596.t1 AT5G65050 32.258 124 67 5 9 122 2 118 6.28e-11 58.9
MS.gene69596.t1 AT4G37940 37.500 88 46 2 9 87 2 89 8.05e-11 59.3

Find 51 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTTTGGCTTCAACAATA+TGG 0.281947 3.1:+40042829 MS.gene69596:CDS
ATCTGAGCTTGTGGAGGATT+TGG 0.315191 3.1:-40042764 None:intergenic
TGGTGGGCTGGTCCCATTGA+TGG 0.319959 3.1:+40042578 MS.gene69596:CDS
TTGTTGATCATCATGTTGAT+AGG 0.343214 3.1:+40042657 MS.gene69596:CDS
AGACGGCCGAGACTCAATTT+TGG 0.343533 3.1:+40042558 MS.gene69596:CDS
ACTTTCTCGAAGCGCCGTAC+TGG 0.372224 3.1:+40042255 MS.gene69596:CDS
ATGTTGCAACCTCATTGGTT+TGG 0.406166 3.1:+40042815 MS.gene69596:CDS
GTCCCATTGATGGGATGAAT+CGG 0.407918 3.1:+40042588 MS.gene69596:CDS
ACCCTATGTTGCAACCTCAT+TGG 0.412021 3.1:+40042810 MS.gene69596:CDS
TGGTTTGGCTTCAACAATAT+GGG 0.414863 3.1:+40042830 MS.gene69596:CDS
TGTTGATAGGCTTGTAATCC+AGG 0.417213 3.1:+40042670 MS.gene69596:CDS
GGTGGGCTGGTCCCATTGAT+GGG 0.419749 3.1:+40042579 MS.gene69596:CDS
TCTTCTCGATGTCCAGTAGC+TGG 0.437748 3.1:-40042505 None:intergenic
CTTCTTGAATAATTCAAGTT+GGG 0.442228 3.1:-40042612 None:intergenic
TGAATTATTCAAGAAGGCTC+TGG 0.442548 3.1:+40042619 MS.gene69596:CDS
GGCCCGATTCATCCCATCAA+TGG 0.457427 3.1:-40042591 None:intergenic
GTGGAGGATTTGGTTGATGA+TGG 0.460225 3.1:-40042754 None:intergenic
TCTCGGCCGTCTTGCGCAAG+TGG 0.485406 3.1:-40042547 None:intergenic
CTTTCTCGAAGCGCCGTACT+GGG 0.485823 3.1:+40042256 MS.gene69596:CDS
GGGTTCTGATAAAACTGTGC+TGG 0.499287 3.1:-40042791 None:intergenic
GCCCGATTCATCCCATCAAT+GGG 0.506203 3.1:-40042590 None:intergenic
ACCAATGAGGTTGCAACATA+GGG 0.507520 3.1:-40042811 None:intergenic
GACTCAGATCAACCAGCTAC+TGG 0.508380 3.1:+40042493 MS.gene69596:intron
TCCCATTGATGGGATGAATC+GGG 0.513278 3.1:+40042589 MS.gene69596:CDS
CCTTCTTGAATAATTCAAGT+TGG 0.516494 3.1:-40042613 None:intergenic
ATGATGGAGAGGCATGTTTG+AGG 0.521462 3.1:-40042738 None:intergenic
AACCAATGAGGTTGCAACAT+AGG 0.525962 3.1:-40042812 None:intergenic
GCTGGAAGCATCTGAGCTTG+TGG 0.533277 3.1:-40042773 None:intergenic
CCAACTTGAATTATTCAAGA+AGG 0.535705 3.1:+40042613 MS.gene69596:CDS
GGGCTCGGATCAAAAGAATC+CGG 0.538178 3.1:-40042876 None:intergenic
AACGAAAATACCTTCTCTCC+AGG 0.551818 3.1:-40042345 None:intergenic
GGAGGTGGATATGGACCCTC+CGG 0.560213 3.1:+40042857 MS.gene69596:CDS
AGCCCACCAAAATTGAGTCT+CGG 0.574024 3.1:-40042564 None:intergenic
GCTGAGCCACTTGCGCAAGA+CGG 0.576977 3.1:+40042541 MS.gene69596:CDS
GGTTTGGCTTCAACAATATG+GGG 0.578983 3.1:+40042831 MS.gene69596:CDS
AGCAACATCTGCACCACAGA+GGG 0.595144 3.1:-40042307 None:intergenic
CTTCAACCATGTCAAGTGGA+AGG 0.599332 3.1:+40042169 None:intergenic
TGTGCTTCAACCATGTCAAG+TGG 0.603608 3.1:+40042165 None:intergenic
ATGTTTGAGTAGGAGCACCC+TGG 0.605264 3.1:-40042688 None:intergenic
AGTGAGCTTTGCACCCTCTG+TGG 0.617361 3.1:+40042294 MS.gene69596:CDS
GGAAGCATCTGAGCTTGTGG+AGG 0.624119 3.1:-40042770 None:intergenic
CTTTCTTGAAGAGCCCAGTA+CGG 0.628289 3.1:-40042269 None:intergenic
GTTGATAGGCTTGTAATCCA+GGG 0.636489 3.1:+40042671 MS.gene69596:CDS
GTTTGGCTTCAACAATATGG+GGG 0.642093 3.1:+40042832 MS.gene69596:CDS
GGATTTGGTTGATGATGGAG+AGG 0.642684 3.1:-40042749 None:intergenic
TTGTTGAAGCCAAACCAATG+AGG 0.650877 3.1:-40042824 None:intergenic
CTCGGATCAAAAGAATCCGG+AGG 0.655674 3.1:-40042873 None:intergenic
TTTGGCTTCAACAATATGGG+GGG 0.656547 3.1:+40042833 MS.gene69596:CDS
TCGGATCAAAAGAATCCGGA+GGG 0.678771 3.1:-40042872 None:intergenic
TCAAGTGGAAGGAAAAGCCG+AGG 0.705721 3.1:+40042180 MS.gene69596:CDS
GAGCAACATCTGCACCACAG+AGG 0.729838 3.1:-40042308 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCATCTCAATCTTT+TGG - chr3.1:40042209-40042228 None:intergenic 20.0%
! AAAAATGGAAATGTTTGAGT+AGG - chr3.1:40042701-40042720 None:intergenic 25.0%
! GAATTGTTTCCAAGAAAAAA+TGG - chr3.1:40042716-40042735 None:intergenic 25.0%
!! CTTCTTGAATAATTCAAGTT+GGG - chr3.1:40042615-40042634 None:intergenic 25.0%
!!! CAAACATTTCCATTTTTTCT+TGG + chr3.1:40042704-40042723 MS.gene69596:CDS 25.0%
CCAACTTGAATTATTCAAGA+AGG + chr3.1:40042613-40042632 MS.gene69596:CDS 30.0%
CCTTCTTGAATAATTCAAGT+TGG - chr3.1:40042616-40042635 None:intergenic 30.0%
TCATAGATCGTTATCTATCT+CGG + chr3.1:40042391-40042410 MS.gene69596:intron 30.0%
TTGTTGATCATCATGTTGAT+AGG + chr3.1:40042657-40042676 MS.gene69596:CDS 30.0%
ATCTATGACCGTATCAACAT+TGG - chr3.1:40042379-40042398 None:intergenic 35.0%
TCTATGACCGTATCAACATT+GGG - chr3.1:40042378-40042397 None:intergenic 35.0%
TGAATTATTCAAGAAGGCTC+TGG + chr3.1:40042619-40042638 MS.gene69596:CDS 35.0%
! ATTGCATGGTACCATTGTTT+TGG - chr3.1:40042424-40042443 None:intergenic 35.0%
! TGGTTTGGCTTCAACAATAT+GGG + chr3.1:40042830-40042849 MS.gene69596:CDS 35.0%
! TTGCATGGTACCATTGTTTT+GGG - chr3.1:40042423-40042442 None:intergenic 35.0%
!! TTGGTTTGGCTTCAACAATA+TGG + chr3.1:40042829-40042848 MS.gene69596:CDS 35.0%
AACCAATGAGGTTGCAACAT+AGG - chr3.1:40042815-40042834 None:intergenic 40.0%
AACGAAAATACCTTCTCTCC+AGG - chr3.1:40042348-40042367 None:intergenic 40.0%
ACCAATGAGGTTGCAACATA+GGG - chr3.1:40042814-40042833 None:intergenic 40.0%
CTATGACCGTATCAACATTG+GGG - chr3.1:40042377-40042396 None:intergenic 40.0%
TGGTACCATGCAATTCATTG+AGG + chr3.1:40042430-40042449 MS.gene69596:intron 40.0%
TTGTTGAAGCCAAACCAATG+AGG - chr3.1:40042827-40042846 None:intergenic 40.0%
! ATGTTGCAACCTCATTGGTT+TGG + chr3.1:40042815-40042834 MS.gene69596:CDS 40.0%
! GGTTTGGCTTCAACAATATG+GGG + chr3.1:40042831-40042850 MS.gene69596:CDS 40.0%
! GTTGATAGGCTTGTAATCCA+GGG + chr3.1:40042671-40042690 MS.gene69596:CDS 40.0%
! GTTTGGCTTCAACAATATGG+GGG + chr3.1:40042832-40042851 MS.gene69596:CDS 40.0%
! TGTTGATAGGCTTGTAATCC+AGG + chr3.1:40042670-40042689 MS.gene69596:CDS 40.0%
! TTTGGCTTCAACAATATGGG+GGG + chr3.1:40042833-40042852 MS.gene69596:CDS 40.0%
!! TGCATGGTACCATTGTTTTG+GGG - chr3.1:40042422-40042441 None:intergenic 40.0%
!!! GGAGAGAAGGTATTTTCGTT+TGG + chr3.1:40042348-40042367 MS.gene69596:intron 40.0%
ACCCTATGTTGCAACCTCAT+TGG + chr3.1:40042810-40042829 MS.gene69596:CDS 45.0%
AGCCCACCAAAATTGAGTCT+CGG - chr3.1:40042567-40042586 None:intergenic 45.0%
ATCTGAGCTTGTGGAGGATT+TGG - chr3.1:40042767-40042786 None:intergenic 45.0%
CTTTCTTGAAGAGCCCAGTA+CGG - chr3.1:40042272-40042291 None:intergenic 45.0%
GTCCCATTGATGGGATGAAT+CGG + chr3.1:40042588-40042607 MS.gene69596:CDS 45.0%
GTGAGCCTCAATGAATTGCA+TGG - chr3.1:40042438-40042457 None:intergenic 45.0%
TCCCATTGATGGGATGAATC+GGG + chr3.1:40042589-40042608 MS.gene69596:CDS 45.0%
! ATGATGGAGAGGCATGTTTG+AGG - chr3.1:40042741-40042760 None:intergenic 45.0%
! GGGTTCTGATAAAACTGTGC+TGG - chr3.1:40042794-40042813 None:intergenic 45.0%
! TCTCAATCTTTTGGCGACCT+CGG - chr3.1:40042200-40042219 None:intergenic 45.0%
! TGTTTTCTCACCTGGAGAGA+AGG + chr3.1:40042335-40042354 MS.gene69596:CDS 45.0%
!! GGATTTGGTTGATGATGGAG+AGG - chr3.1:40042752-40042771 None:intergenic 45.0%
!! GTGGAGGATTTGGTTGATGA+TGG - chr3.1:40042757-40042776 None:intergenic 45.0%
!!! ATGGTACCATTGTTTTGGGG+TGG - chr3.1:40042419-40042438 None:intergenic 45.0%
AGCAACATCTGCACCACAGA+GGG - chr3.1:40042310-40042329 None:intergenic 50.0%
GACTCAGATCAACCAGCTAC+TGG + chr3.1:40042493-40042512 MS.gene69596:intron 50.0%
GCCCGATTCATCCCATCAAT+GGG - chr3.1:40042593-40042612 None:intergenic 50.0%
GTAGCTGGTTGATCTGAGTC+AGG - chr3.1:40042493-40042512 None:intergenic 50.0%
TCAAGTGGAAGGAAAAGCCG+AGG + chr3.1:40042180-40042199 MS.gene69596:CDS 50.0%
TCGGATCCACCCCAAAACAA+TGG + chr3.1:40042410-40042429 MS.gene69596:intron 50.0%
TCTTCTCGATGTCCAGTAGC+TGG - chr3.1:40042508-40042527 None:intergenic 50.0%
TGACCGTATCAACATTGGGG+TGG - chr3.1:40042374-40042393 None:intergenic 50.0%
TGGCCACCCCAATGTTGATA+CGG + chr3.1:40042368-40042387 MS.gene69596:intron 50.0%
TTCAACAATATGGGGGGAGG+AGG + chr3.1:40042839-40042858 MS.gene69596:CDS 50.0%
! AGACGGCCGAGACTCAATTT+TGG + chr3.1:40042558-40042577 MS.gene69596:CDS 50.0%
! AGTATTGAGCTCACGCACGT+TGG - chr3.1:40042468-40042487 None:intergenic 50.0%
!! ATGTTTGAGTAGGAGCACCC+TGG - chr3.1:40042691-40042710 None:intergenic 50.0%
!! GAGACTCAATTTTGGTGGGC+TGG + chr3.1:40042566-40042585 MS.gene69596:CDS 50.0%
!! GCTCTCGTTGTTTTCTCACC+TGG + chr3.1:40042327-40042346 MS.gene69596:CDS 50.0%
!! GGCCGAGACTCAATTTTGGT+GGG + chr3.1:40042562-40042581 MS.gene69596:CDS 50.0%
AACAATATGGGGGGAGGAGG+TGG + chr3.1:40042842-40042861 MS.gene69596:CDS 55.0%
AGTGAGCTTTGCACCCTCTG+TGG + chr3.1:40042294-40042313 MS.gene69596:CDS 55.0%
GAGCAACATCTGCACCACAG+AGG - chr3.1:40042311-40042330 None:intergenic 55.0%
GGCCCGATTCATCCCATCAA+TGG - chr3.1:40042594-40042613 None:intergenic 55.0%
GGCTTCAACAATATGGGGGG+AGG + chr3.1:40042836-40042855 MS.gene69596:CDS 55.0%
! CGGCCGAGACTCAATTTTGG+TGG + chr3.1:40042561-40042580 MS.gene69596:CDS 55.0%
!! ACTTTCTCGAAGCGCCGTAC+TGG + chr3.1:40042255-40042274 MS.gene69596:CDS 55.0%
!! CTTTCTCGAAGCGCCGTACT+GGG + chr3.1:40042256-40042275 MS.gene69596:CDS 55.0%
!! GCTGGAAGCATCTGAGCTTG+TGG - chr3.1:40042776-40042795 None:intergenic 55.0%
!! GGAAGCATCTGAGCTTGTGG+AGG - chr3.1:40042773-40042792 None:intergenic 55.0%
ATGGGGGGAGGAGGTGGATA+TGG + chr3.1:40042848-40042867 MS.gene69596:CDS 60.0%
GCTGAGCCACTTGCGCAAGA+CGG + chr3.1:40042541-40042560 MS.gene69596:CDS 60.0%
GGAGGTGGATATGGACCCTC+CGG + chr3.1:40042857-40042876 MS.gene69596:CDS 60.0%
!! GGTGGGCTGGTCCCATTGAT+GGG + chr3.1:40042579-40042598 MS.gene69596:CDS 60.0%
!! TGGTGGGCTGGTCCCATTGA+TGG + chr3.1:40042578-40042597 MS.gene69596:CDS 60.0%
GCTCACGCACGTTGGCGCTA+CGG - chr3.1:40042460-40042479 None:intergenic 65.0%
TCTCGGCCGTCTTGCGCAAG+TGG - chr3.1:40042550-40042569 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 40042177 40042889 40042177 ID=MS.gene69596
chr3.1 mRNA 40042177 40042889 40042177 ID=MS.gene69596.t1;Parent=MS.gene69596
chr3.1 exon 40042177 40042356 40042177 ID=MS.gene69596.t1.exon1;Parent=MS.gene69596.t1
chr3.1 CDS 40042177 40042356 40042177 ID=cds.MS.gene69596.t1;Parent=MS.gene69596.t1
chr3.1 exon 40042500 40042889 40042500 ID=MS.gene69596.t1.exon2;Parent=MS.gene69596.t1
chr3.1 CDS 40042500 40042889 40042500 ID=cds.MS.gene69596.t1;Parent=MS.gene69596.t1
Gene Sequence

>MS.gene69596

ATGTCAAGTGGAAGGAAAAGCCGAGGTCGCCAAAAGATTGAGATGAAAAAAATGAGCAACGAGAGCAACTTGCAAGTGACTTTCTCGAAGCGCCGTACTGGGCTCTTCAAGAAAGTCAGTGAGCTTTGCACCCTCTGTGGTGCAGATGTTGCTCTCGTTGTTTTCTCACCTGGAGAGAAGGTATTTTCGTTTGGCCACCCCAATGTTGATACGGTCATAGATCGTTATCTATCTCGGATCCACCCCAAAACAATGGTACCATGCAATTCATTGAGGCTCACCGTAGCGCCAACGTGCGTGAGCTCAATACTCACCTGACTCAGATCAACCAGCTACTGGACATCGAGAAGAAACGTGCTGAAGAGCTGAGCCACTTGCGCAAGACGGCCGAGACTCAATTTTGGTGGGCTGGTCCCATTGATGGGATGAATCGGGCCCAACTTGAATTATTCAAGAAGGCTCTGGATGAGCTTAAGAAAATTGTTGATCATCATGTTGATAGGCTTGTAATCCAGGGTGCTCCTACTCAAACATTTCCATTTTTTCTTGGAAACAATTCATCCTCAAACATGCCTCTCCATCATCAACCAAATCCTCCACAAGCTCAGATGCTTCCAGCACAGTTTTATCAGAACCCTATGTTGCAACCTCATTGGTTTGGCTTCAACAATATGGGGGGAGGAGGTGGATATGGACCCTCCGGATTCTTTTGA
Protein sequence

>MS.gene69596.t1

MSSGRKSRGRQKIEMKKMSNESNLQVTFSKRRTGLFKKVSELCTLCGADVALVVFSPGEKINQLLDIEKKRAEELSHLRKTAETQFWWAGPIDGMNRAQLELFKKALDELKKIVDHHVDRLVIQGAPTQTFPFFLGNNSSSNMPLHHQPNPPQAQMLPAQFYQNPMLQPHWFGFNNMGGGGGYGPSGFF