AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70060.t1 MTR_5g046410 93.548 124 8 0 1 124 1 124 1.92e-81 238
MS.gene70060.t1 MTR_5g046430 93.548 124 8 0 1 124 1 124 1.92e-81 238
MS.gene70060.t1 MTR_4g017030 93.548 124 8 0 1 124 1 124 1.92e-81 238
MS.gene70060.t1 MTR_4g017050 92.742 124 9 0 1 124 1 124 7.40e-81 237
MS.gene70060.t1 MTR_2g019250 91.129 124 11 0 1 124 1 124 2.52e-80 236
MS.gene70060.t1 MTR_4g017040 91.129 124 11 0 1 124 1 124 6.98e-80 234
MS.gene70060.t1 MTR_1g079490 71.200 125 35 1 1 124 1 125 7.91e-61 186
MS.gene70060.t1 MTR_6g005310 67.769 121 38 1 4 124 5 124 1.35e-53 168
MS.gene70060.t1 MTR_6g005340 67.769 121 38 1 4 124 5 124 2.26e-53 167
MS.gene70060.t1 MTR_6g005330 67.200 125 39 2 1 124 1 124 3.10e-53 167
MS.gene70060.t1 MTR_6g005360 63.710 124 45 0 1 124 1 124 4.29e-53 167
MS.gene70060.t1 MTR_6g005380 59.167 120 49 0 1 120 1 120 1.54e-47 152
MS.gene70060.t1 MTR_6g005350 58.475 118 48 1 3 120 4 120 1.32e-41 137
MS.gene70060.t1 MTR_1g023770 54.098 122 46 2 1 120 1 114 3.27e-33 115
MS.gene70060.t1 MTR_1g085500 50.000 118 55 2 5 120 6 121 2.22e-30 108
MS.gene70060.t1 MTR_2g030855 57.143 105 36 3 19 120 19 117 6.68e-30 107
MS.gene70060.t1 MTR_1g085140 50.476 105 48 2 18 120 20 122 2.00e-27 101
MS.gene70060.t1 MTR_2g031270 51.613 124 50 4 1 120 1 118 7.37e-27 99.8
MS.gene70060.t1 MTR_7g111240 51.485 101 45 2 22 120 20 118 4.99e-25 95.1
MS.gene70060.t1 MTR_2g031300 63.636 77 24 2 47 120 7 82 5.30e-25 93.2
MS.gene70060.t1 MTR_2g030895 63.636 77 24 2 47 120 7 82 1.46e-24 92.8
MS.gene70060.t1 MTR_7g111240 51.485 101 42 2 22 120 20 115 8.50e-24 91.7
MS.gene70060.t1 MTR_2g030865 61.039 77 26 2 47 120 7 82 2.20e-22 87.4
MS.gene70060.t1 MTR_8g007300 46.465 99 50 2 22 118 26 123 3.57e-19 79.7
MS.gene70060.t1 MTR_5g036570 41.584 101 55 2 22 120 27 125 1.11e-18 78.6
MS.gene70060.t1 MTR_1g060120 33.846 130 79 3 2 124 5 134 1.54e-17 75.9
MS.gene70060.t1 MTR_1g112200 37.615 109 59 3 19 125 20 121 4.86e-17 73.9
MS.gene70060.t1 MTR_3g019710 40.984 122 63 4 1 120 1 115 3.31e-16 72.0
MS.gene70060.t1 MTR_2g072560 34.328 134 79 4 1 132 1 127 3.77e-16 70.5
MS.gene70060.t1 MTR_2g041550 38.525 122 63 3 4 123 7 118 3.99e-16 71.6
MS.gene70060.t1 MTR_2g041570 36.885 122 65 3 4 123 7 118 4.47e-16 71.6
MS.gene70060.t1 MTR_2g041580 36.885 122 65 3 4 123 7 118 5.13e-16 71.2
MS.gene70060.t1 MTR_8g020630 37.600 125 69 4 1 123 1 118 1.60e-15 70.1
MS.gene70060.t1 MTR_3g096330 36.441 118 72 2 5 120 560 676 2.07e-14 68.9
MS.gene70060.t1 MTR_2g036120 36.800 125 70 4 1 123 1 118 4.95e-14 66.2
MS.gene70060.t1 MTR_5g031460 33.871 124 75 3 1 123 1 118 7.32e-13 63.2
MS.gene70060.t1 MTR_2g045100 37.600 125 69 4 1 123 1 118 1.02e-12 62.8
MS.gene70060.t1 MTR_2g044040 36.000 125 71 4 1 123 1 118 1.16e-12 62.4
MS.gene70060.t1 MTR_8g020650 36.800 125 70 4 1 123 1 118 1.30e-12 62.4
MS.gene70060.t1 MTR_8g020590 34.400 125 70 4 1 123 2 116 2.06e-12 61.6
MS.gene70060.t1 MTR_3g019680 36.290 124 65 5 1 120 1 114 3.60e-12 61.2
MS.gene70060.t1 MTR_3g019610 37.500 112 61 4 1 110 1 105 3.82e-12 58.9
MS.gene70060.t1 MTR_0284s0010 36.066 122 69 4 1 120 1 115 3.84e-12 61.2
MS.gene70060.t1 MTR_3g019650 36.290 124 65 5 1 120 1 114 4.04e-12 60.8
MS.gene70060.t1 MTR_8g020770 36.585 123 70 3 1 120 1 118 4.47e-12 60.8
MS.gene70060.t1 MTR_8g020670 36.220 127 68 5 1 123 1 118 7.29e-12 60.5
MS.gene70060.t1 MTR_8g020750 35.385 130 68 4 1 123 1 121 1.69e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70060.t1 AT5G39150 59.055 127 49 2 1 124 1 127 7.42e-47 151
MS.gene70060.t1 AT5G39180 59.055 127 49 2 1 124 1 127 8.00e-47 150
MS.gene70060.t1 AT5G39120 60.833 120 44 2 7 124 9 127 4.51e-46 149
MS.gene70060.t1 AT3G05950 63.115 122 39 3 4 120 5 125 2.53e-45 147
MS.gene70060.t1 AT5G39110 60.000 120 46 1 7 124 9 128 5.84e-45 146
MS.gene70060.t1 AT4G14630 57.258 124 49 2 1 120 3 126 8.02e-44 143
MS.gene70060.t1 AT5G39160 59.322 118 45 2 9 124 11 127 5.14e-43 141
MS.gene70060.t1 AT5G39190 59.322 118 45 2 9 124 11 127 7.04e-43 140
MS.gene70060.t1 AT5G39130 59.322 118 45 2 9 124 11 127 7.51e-43 140
MS.gene70060.t1 AT5G39160 53.125 128 51 3 1 124 1 123 3.55e-38 129
MS.gene70060.t1 AT5G38910 53.846 130 54 3 1 125 1 129 2.02e-37 125
MS.gene70060.t1 AT3G04200 54.918 122 52 3 1 120 6 126 2.54e-37 127
MS.gene70060.t1 AT5G38910 56.911 123 47 3 1 118 1 122 3.44e-37 126
MS.gene70060.t1 AT3G04150 49.640 139 60 4 3 132 4 141 1.30e-36 125
MS.gene70060.t1 AT5G38960 54.545 110 47 2 18 124 22 131 8.68e-36 122
MS.gene70060.t1 AT3G04170 50.382 131 55 4 8 132 12 138 1.70e-35 122
MS.gene70060.t1 AT3G04150 45.695 151 61 4 3 132 4 154 1.22e-34 120
MS.gene70060.t1 AT3G04190 50.000 112 53 2 15 124 19 129 4.05e-34 118
MS.gene70060.t1 AT5G38930 58.416 101 40 1 20 118 24 124 8.39e-34 117
MS.gene70060.t1 AT5G38940 58.586 99 39 1 22 118 26 124 9.97e-34 117
MS.gene70060.t1 AT5G38940 58.586 99 39 1 22 118 50 148 1.25e-33 118
MS.gene70060.t1 AT3G04180 45.968 124 60 3 15 132 19 141 1.05e-31 112
MS.gene70060.t1 AT5G39190 46.825 126 44 2 1 124 1 105 8.57e-31 109
MS.gene70060.t1 AT5G39160 46.032 126 45 2 1 124 1 105 2.32e-30 108
MS.gene70060.t1 AT3G62020 47.863 117 56 3 6 120 8 121 1.08e-28 104
MS.gene70060.t1 AT3G05930 44.800 125 63 3 3 124 5 126 4.17e-27 100
MS.gene70060.t1 AT1G09560 47.170 106 52 2 17 120 19 122 6.15e-26 97.4
MS.gene70060.t1 AT5G26700 47.573 103 46 3 26 124 25 123 1.56e-25 96.3
MS.gene70060.t1 AT1G02335 47.573 103 50 2 20 120 22 122 1.99e-24 93.6
MS.gene70060.t1 AT3G62020 50.000 84 40 1 39 120 9 92 4.69e-21 84.3
MS.gene70060.t1 AT1G10460 40.777 103 57 3 22 120 19 121 4.64e-20 82.0
MS.gene70060.t1 AT5G61750 39.496 119 70 1 4 120 1 119 5.52e-20 81.6
MS.gene70060.t1 AT1G18970 42.202 109 57 3 18 124 25 129 7.60e-19 79.0
MS.gene70060.t1 AT5G20630 40.800 125 66 4 1 123 1 119 7.73e-19 79.0
MS.gene70060.t1 AT5G39100 72.000 50 14 0 7 56 9 58 1.19e-18 74.3
MS.gene70060.t1 AT1G18980 42.202 109 57 3 18 124 25 129 5.02e-18 77.0
MS.gene70060.t1 AT3G10080 35.714 112 68 3 18 125 22 133 1.62e-16 73.2
MS.gene70060.t1 AT3G10080 36.036 111 67 3 18 124 93 203 2.68e-16 73.6
MS.gene70060.t1 AT1G72610 44.000 100 52 3 26 123 19 116 8.29e-15 68.2

Find 42 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATACATACCACCATCTT+TGG 0.255130 4.2:-84924369 None:intergenic
GTCACTGCAGACCAACTATT+TGG 0.269897 4.2:+84925559 MS.gene70060:CDS
CCTTGTTAGCATCTTGGCTT+TGG 0.311083 4.2:+84924280 MS.gene70060:CDS
TTGAGAGCCTAGTGCGTTAG+AGG 0.314204 4.2:-84925527 None:intergenic
CAACACACTTGGGATATCTT+TGG 0.340651 4.2:+84925585 MS.gene70060:CDS
ACCATCTTTGGGGTCTTTGA+TGG 0.343346 4.2:-84924357 None:intergenic
TAGTACTTGAATGTGGGATA+TGG 0.377931 4.2:+84925677 MS.gene70060:CDS
ATACTTCCTTGTTAGCATCT+TGG 0.401215 4.2:+84924274 MS.gene70060:CDS
TTCTTCAAACATGTTGAAGC+TGG 0.402098 4.2:+84925499 MS.gene70060:CDS
TCCCACATTCAAGTACTATA+AGG 0.403633 4.2:-84925672 None:intergenic
ATATACATACCACCATCTTT+GGG 0.405165 4.2:-84924368 None:intergenic
TATTTGCAGTGTTTGTGAAC+GGG 0.415812 4.2:+84925437 MS.gene70060:intron
CAAAAGTCTTGGAGAGGGCT+AGG 0.434671 4.2:-84924329 None:intergenic
CTATTTGGACTCAACACACT+TGG 0.473975 4.2:+84925574 MS.gene70060:CDS
ATAAGGATTTCCGTTCCTCT+AGG 0.478305 4.2:-84925655 None:intergenic
GGCAACGCTGTTGCTATCGC+TGG 0.484128 4.2:+84925698 MS.gene70060:CDS
TCTTCAGCTTTAACTAGCGC+GGG 0.484814 4.2:-84925475 None:intergenic
CTCTAGGGTGAATGTGAGGT+GGG 0.498933 4.2:-84925639 None:intergenic
TGATGGCAACACAAAAGTCT+TGG 0.500181 4.2:-84924340 None:intergenic
TCTTCAAACATGTTGAAGCT+GGG 0.501315 4.2:+84925500 MS.gene70060:CDS
ATCCTTATAGTACTTGAATG+TGG 0.530485 4.2:+84925670 MS.gene70060:CDS
TCCTTATAGTACTTGAATGT+GGG 0.535269 4.2:+84925671 MS.gene70060:CDS
TGAGGTGGGTTTAAACCCTT+AGG 0.540358 4.2:-84925625 None:intergenic
ATCTTCAGCTTTAACTAGCG+CGG 0.543736 4.2:-84925476 None:intergenic
TAAGGATTTCCGTTCCTCTA+GGG 0.546102 4.2:-84925654 None:intergenic
TTATTTGCAGTGTTTGTGAA+CGG 0.552025 4.2:+84925436 MS.gene70060:intron
GCCATCAAAGACCCCAAAGA+TGG 0.552100 4.2:+84924356 MS.gene70060:CDS
CCAAAGCCAAGATGCTAACA+AGG 0.556137 4.2:-84924280 None:intergenic
TATTTGGACTCAACACACTT+GGG 0.557339 4.2:+84925575 MS.gene70060:CDS
AAAAGTCTTGGAGAGGGCTA+GGG 0.564154 4.2:-84924328 None:intergenic
CCTCTAGGGTGAATGTGAGG+TGG 0.565620 4.2:-84925640 None:intergenic
GTGTGTTGAGTCCAAATAGT+TGG 0.570150 4.2:-84925570 None:intergenic
GGGAATGCCTCTAACGCACT+AGG 0.573570 4.2:+84925520 MS.gene70060:CDS
AGGGCTAGGGTCATAAGCAA+AGG 0.622370 4.2:-84924315 None:intergenic
TCACATTCACCCTAGAGGAA+CGG 0.635178 4.2:+84925645 MS.gene70060:CDS
ATCAAAGACCCCAAAGATGG+TGG 0.635231 4.2:+84924359 MS.gene70060:CDS
TATACATACCACCATCTTTG+GGG 0.639620 4.2:-84924367 None:intergenic
CAACACAAAAGTCTTGGAGA+GGG 0.646061 4.2:-84924334 None:intergenic
GCAACACAAAAGTCTTGGAG+AGG 0.651085 4.2:-84924335 None:intergenic
CTTCAGCTTTAACTAGCGCG+GGG 0.662073 4.2:-84925474 None:intergenic
GTTCCTCTAGGGTGAATGTG+AGG 0.681575 4.2:-84925643 None:intergenic
CCACCTCACATTCACCCTAG+AGG 0.685266 4.2:+84925640 MS.gene70060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAGAGAAAATTTATA+TGG - chr4.2:84925296-84925315 None:intergenic 10.0%
!! ATATTTAGTATTATAGTATA+TGG + chr4.2:84925227-84925246 MS.gene70060:intron 10.0%
!! ATTAAAATGATGATTTAATT+TGG - chr4.2:84924739-84924758 None:intergenic 10.0%
!!! TATTTTTTTACTAAATAATG+GGG - chr4.2:84924587-84924606 None:intergenic 10.0%
!! ATTATTTGTATGTACTTAAT+AGG - chr4.2:84924998-84925017 None:intergenic 15.0%
!! TAAATGAAATATGAATTAGT+TGG - chr4.2:84924640-84924659 None:intergenic 15.0%
!! TAATAACCTTATAGTAATAA+TGG - chr4.2:84925154-84925173 None:intergenic 15.0%
!!! AAATATAGAATCTTTTGTTT+TGG + chr4.2:84925334-84925353 MS.gene70060:intron 15.0%
!!! AATAGGATTCTAAAAAATAT+TGG - chr4.2:84924917-84924936 None:intergenic 15.0%
!!! ATTTTTTTACTAAATAATGG+GGG - chr4.2:84924586-84924605 None:intergenic 15.0%
!!! TTATTTTACTATAGTAAAGT+TGG + chr4.2:84924613-84924632 MS.gene70060:intron 15.0%
!! ATACGAAAATTAATAAGTAG+TGG - chr4.2:84924535-84924554 None:intergenic 20.0%
!! CTGTTGAACAAAAAAAAAAT+GGG - chr4.2:84924888-84924907 None:intergenic 20.0%
!! TAATCAAGACTTAAAAGTTA+AGG - chr4.2:84924397-84924416 None:intergenic 20.0%
!!! CATTTTTTTTTTGTTCAACA+GGG + chr4.2:84924887-84924906 MS.gene70060:intron 20.0%
! ACATTTCAATAAATACCAAG+TGG - chr4.2:84925078-84925097 None:intergenic 25.0%
! AGAGAAAATTTATATGGAGT+CGG - chr4.2:84925290-84925309 None:intergenic 25.0%
! CCTATTTAACTAAAAAAGTC+AGG + chr4.2:84924931-84924950 MS.gene70060:intron 25.0%
! CCTGTTGAACAAAAAAAAAA+TGG - chr4.2:84924889-84924908 None:intergenic 25.0%
! TCACTTCCATTATTACTATA+AGG + chr4.2:84925145-84925164 MS.gene70060:intron 25.0%
!! AACCATAGATAAAGTCTTTT+AGG + chr4.2:84924959-84924978 MS.gene70060:intron 25.0%
!!! ATTTTAGCAGTTTTTAGTCA+TGG - chr4.2:84925387-84925406 None:intergenic 25.0%
!!! CCATTTTTTTTTTGTTCAAC+AGG + chr4.2:84924886-84924905 MS.gene70060:intron 25.0%
!!! CCTGACTTTTTTAGTTAAAT+AGG - chr4.2:84924934-84924953 None:intergenic 25.0%
AAGACTTTATCTATGGTTTG+TGG - chr4.2:84924957-84924976 None:intergenic 30.0%
ATATACATACCACCATCTTT+GGG - chr4.2:84924371-84924390 None:intergenic 30.0%
ATCCTTATAGTACTTGAATG+TGG + chr4.2:84925670-84925689 MS.gene70060:CDS 30.0%
CGAAAATTAATAAGTAGTGG+AGG - chr4.2:84924532-84924551 None:intergenic 30.0%
TATATACATACCACCATCTT+TGG - chr4.2:84924372-84924391 None:intergenic 30.0%
TCCTTATAGTACTTGAATGT+GGG + chr4.2:84925671-84925690 MS.gene70060:CDS 30.0%
TTATTTGCAGTGTTTGTGAA+CGG + chr4.2:84925436-84925455 MS.gene70060:intron 30.0%
! AGCCAGAAATTTTAGATTGT+AGG - chr4.2:84925261-84925280 None:intergenic 30.0%
! CAAAAAGTCTAAGATTTGCA+GGG - chr4.2:84924458-84924477 None:intergenic 30.0%
! CCAGAAATTTTAGATTGTAG+GGG - chr4.2:84925259-84925278 None:intergenic 30.0%
! GCCAGAAATTTTAGATTGTA+GGG - chr4.2:84925260-84925279 None:intergenic 30.0%
!!! GAATCTTTTGTTTTGGTTCA+CGG + chr4.2:84925341-84925360 MS.gene70060:intron 30.0%
ATACTTCCTTGTTAGCATCT+TGG + chr4.2:84924274-84924293 MS.gene70060:CDS 35.0%
GAAAATTTATATGGAGTCGG+TGG - chr4.2:84925287-84925306 None:intergenic 35.0%
GGCCTAAAAGACTTTATCTA+TGG - chr4.2:84924964-84924983 None:intergenic 35.0%
TAGTACTTGAATGTGGGATA+TGG + chr4.2:84925677-84925696 MS.gene70060:CDS 35.0%
TATACATACCACCATCTTTG+GGG - chr4.2:84924370-84924389 None:intergenic 35.0%
TATTTGCAGTGTTTGTGAAC+GGG + chr4.2:84925437-84925456 MS.gene70060:intron 35.0%
TATTTGGACTCAACACACTT+GGG + chr4.2:84925575-84925594 MS.gene70060:CDS 35.0%
TCCCACATTCAAGTACTATA+AGG - chr4.2:84925675-84925694 None:intergenic 35.0%
TCTTCAAACATGTTGAAGCT+GGG + chr4.2:84925500-84925519 MS.gene70060:CDS 35.0%
TTCTTCAAACATGTTGAAGC+TGG + chr4.2:84925499-84925518 MS.gene70060:CDS 35.0%
! CCAAAAAGTCTAAGATTTGC+AGG - chr4.2:84924459-84924478 None:intergenic 35.0%
! CCCCTACAATCTAAAATTTC+TGG + chr4.2:84925256-84925275 MS.gene70060:intron 35.0%
! CCTGCAAATCTTAGACTTTT+TGG + chr4.2:84924456-84924475 MS.gene70060:intron 35.0%
! TAAAGTCTTTTAGGCCAACA+AGG + chr4.2:84924968-84924987 MS.gene70060:intron 35.0%
!!! TTTGGTTTTACTCCATACAG+TGG + chr4.2:84924474-84924493 MS.gene70060:intron 35.0%
AATTTATATGGAGTCGGTGG+CGG - chr4.2:84925284-84925303 None:intergenic 40.0%
ATAAGGATTTCCGTTCCTCT+AGG - chr4.2:84925658-84925677 None:intergenic 40.0%
CAACACAAAAGTCTTGGAGA+GGG - chr4.2:84924337-84924356 None:intergenic 40.0%
CAACACACTTGGGATATCTT+TGG + chr4.2:84925585-84925604 MS.gene70060:CDS 40.0%
CTATTTGGACTCAACACACT+TGG + chr4.2:84925574-84925593 MS.gene70060:CDS 40.0%
GTGTGTTGAGTCCAAATAGT+TGG - chr4.2:84925573-84925592 None:intergenic 40.0%
TAAGGATTTCCGTTCCTCTA+GGG - chr4.2:84925657-84925676 None:intergenic 40.0%
TGATGGCAACACAAAAGTCT+TGG - chr4.2:84924343-84924362 None:intergenic 40.0%
! AAAATGGGCTCACCTAATGT+AGG - chr4.2:84924873-84924892 None:intergenic 40.0%
! AAATAATGGGGGCATGAAGT+TGG - chr4.2:84924575-84924594 None:intergenic 40.0%
! ATCTTCAGCTTTAACTAGCG+CGG - chr4.2:84925479-84925498 None:intergenic 40.0%
!! GAAGTCACTGACTTGAGAAT+TGG - chr4.2:84925056-84925075 None:intergenic 40.0%
AAAAGTCTTGGAGAGGGCTA+GGG - chr4.2:84924331-84924350 None:intergenic 45.0%
ACTTAATAGGCCGACCTTGT+TGG - chr4.2:84924985-84925004 None:intergenic 45.0%
AGCTACATGTACCCTTGTGT+GGG + chr4.2:84924500-84924519 MS.gene70060:intron 45.0%
ATCAAAGACCCCAAAGATGG+TGG + chr4.2:84924359-84924378 MS.gene70060:CDS 45.0%
ATGTAGGCCAGGTTAAAGGT+CGG - chr4.2:84924857-84924876 None:intergenic 45.0%
CCAAAGCCAAGATGCTAACA+AGG - chr4.2:84924283-84924302 None:intergenic 45.0%
CCTAATGTAGGCCAGGTTAA+AGG - chr4.2:84924861-84924880 None:intergenic 45.0%
CCTTGTTAGCATCTTGGCTT+TGG + chr4.2:84924280-84924299 MS.gene70060:CDS 45.0%
CCTTTAACCTGGCCTACATT+AGG + chr4.2:84924858-84924877 MS.gene70060:intron 45.0%
GCAACACAAAAGTCTTGGAG+AGG - chr4.2:84924338-84924357 None:intergenic 45.0%
GGAATAGGCTGAGTCAAGTT+AGG - chr4.2:84925029-84925048 None:intergenic 45.0%
GTCACTGCAGACCAACTATT+TGG + chr4.2:84925559-84925578 MS.gene70060:CDS 45.0%
TCAAGTCAGTGACTTCCACT+TGG + chr4.2:84925060-84925079 MS.gene70060:intron 45.0%
TCACATTCACCCTAGAGGAA+CGG + chr4.2:84925645-84925664 MS.gene70060:CDS 45.0%
TTGAGAATTGGAGCGGGAAT+AGG - chr4.2:84925044-84925063 None:intergenic 45.0%
! GGTTTTACTCCATACAGTGG+CGG + chr4.2:84924477-84924496 MS.gene70060:intron 45.0%
! GTCTTTTAGGCCAACAAGGT+CGG + chr4.2:84924972-84924991 MS.gene70060:intron 45.0%
! TCTTCAGCTTTAACTAGCGC+GGG - chr4.2:84925478-84925497 None:intergenic 45.0%
! TGAGGTGGGTTTAAACCCTT+AGG - chr4.2:84925628-84925647 None:intergenic 45.0%
!! ACCATCTTTGGGGTCTTTGA+TGG - chr4.2:84924360-84924379 None:intergenic 45.0%
!! ACTGACTTGAGAATTGGAGC+GGG - chr4.2:84925050-84925069 None:intergenic 45.0%
!! CACTGACTTGAGAATTGGAG+CGG - chr4.2:84925051-84925070 None:intergenic 45.0%
!!! CGCGTTGATTTTGAGCCTAA+GGG + chr4.2:84925610-84925629 MS.gene70060:CDS 45.0%
!!! TCGCGTTGATTTTGAGCCTA+AGG + chr4.2:84925609-84925628 MS.gene70060:CDS 45.0%
!!! TTTTGGTTCACGGTGTTTCC+TGG + chr4.2:84925351-84925370 MS.gene70060:intron 45.0%
!! AAAAAAAAAATACATATTAT+AGG - chr4.2:84925192-84925211 None:intergenic 5.0%
!!! TTATTTTTTTACTAAATAAT+GGG - chr4.2:84924588-84924607 None:intergenic 5.0%
!!! TTTATTTTTTTACTAAATAA+TGG - chr4.2:84924589-84924608 None:intergenic 5.0%
AGGGCTAGGGTCATAAGCAA+AGG - chr4.2:84924318-84924337 None:intergenic 50.0%
AGTCATGGCCTAGCTAAACC+AGG - chr4.2:84925372-84925391 None:intergenic 50.0%
CAAAAGTCTTGGAGAGGGCT+AGG - chr4.2:84924332-84924351 None:intergenic 50.0%
CGGTGTTTCCTGGTTTAGCT+AGG + chr4.2:84925361-84925380 MS.gene70060:intron 50.0%
GAGCTACATGTACCCTTGTG+TGG + chr4.2:84924499-84924518 MS.gene70060:intron 50.0%
GCCATCAAAGACCCCAAAGA+TGG + chr4.2:84924356-84924375 MS.gene70060:CDS 50.0%
TGTAGGCCAGGTTAAAGGTC+GGG - chr4.2:84924856-84924875 None:intergenic 50.0%
TGTCAACCCGACCTTTAACC+TGG + chr4.2:84924847-84924866 MS.gene70060:intron 50.0%
TTGAGAGCCTAGTGCGTTAG+AGG - chr4.2:84925530-84925549 None:intergenic 50.0%
! CTTCAGCTTTAACTAGCGCG+GGG - chr4.2:84925477-84925496 None:intergenic 50.0%
!! CTCTAGGGTGAATGTGAGGT+GGG - chr4.2:84925642-84925661 None:intergenic 50.0%
!! GTTCCTCTAGGGTGAATGTG+AGG - chr4.2:84925646-84925665 None:intergenic 50.0%
CATGTAGCTCCGCCACTGTA+TGG - chr4.2:84924489-84924508 None:intergenic 55.0%
CCACCTCACATTCACCCTAG+AGG + chr4.2:84925640-84925659 MS.gene70060:CDS 55.0%
GGGAATGCCTCTAACGCACT+AGG + chr4.2:84925520-84925539 MS.gene70060:CDS 55.0%
!! CCTCTAGGGTGAATGTGAGG+TGG - chr4.2:84925643-84925662 None:intergenic 55.0%
GGCAACGCTGTTGCTATCGC+TGG + chr4.2:84925698-84925717 MS.gene70060:CDS 60.0%
GGGCTCACCTAATGTAGGCC+AGG - chr4.2:84924868-84924887 None:intergenic 60.0%
! GGAGGCACATGCCCACACAA+GGG - chr4.2:84924514-84924533 None:intergenic 60.0%
! TGGAGGCACATGCCCACACA+AGG - chr4.2:84924515-84924534 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 84924263 84925726 84924263 ID=MS.gene70060
chr4.2 mRNA 84924263 84925726 84924263 ID=MS.gene70060.t1;Parent=MS.gene70060
chr4.2 exon 84924263 84924380 84924263 ID=MS.gene70060.t1.exon1;Parent=MS.gene70060.t1
chr4.2 CDS 84924263 84924380 84924263 ID=cds.MS.gene70060.t1;Parent=MS.gene70060.t1
chr4.2 exon 84925446 84925726 84925446 ID=MS.gene70060.t1.exon2;Parent=MS.gene70060.t1
chr4.2 CDS 84925446 84925726 84925446 ID=cds.MS.gene70060.t1;Parent=MS.gene70060.t1
Gene Sequence

>MS.gene70060

ATGAAGATTATATACTTCCTTGTTAGCATCTTGGCTTTGGCATCATCTGTTGCCTTTGCTTATGACCCTAGCCCTCTCCAAGACTTTTGTGTTGCCATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAACTTTTAAGTCTTGATTAATTACTACTCAATTTTTAAGCTTAAATATGCAAATGTTCCCTGCAAATCTTAGACTTTTTGGTTTTACTCCATACAGTGGCGGAGCTACATGTACCCTTGTGTGGGCATGTGCCTCCACTACTTATTAATTTTCGTATATAACATTATTGTTAAGCCAACTTCATGCCCCCATTATTTAGTAAAAAAATAAAAAATTTATTTTACTATAGTAAAGTTGGTCCAACTAATTCATATTTCATTTATTGATAAATTGATTCATTAATCATAACATATATTTCTTTTGTGCTATAAGTGATTCATTTGTAATAAAAATTTGTTCCAAATTAAATCATCATTTTAATTTTCAATGCAATCATAGACACTTTTTTTTTTTTATTAATATACACTAATAGTTGCATTATTTGCATTTTGCAAGTACATGTGACAACATGTCAACCCGACCTTTAACCTGGCCTACATTAGGTGAGCCCATTTTTTTTTTGTTCAACAGGGAATACCAATATTTTTTAGAATCCTATTTAACTAAAAAAGTCAGGCCACAAACCATAGATAAAGTCTTTTAGGCCAACAAGGTCGGCCTATTAAGTACATACAAATAATTTGCAAAACCTAACTTGACTCAGCCTATTCCCGCTCCAATTCTCAAGTCAGTGACTTCCACTTGGTATTTATTGAAATGTAAATAAATTAATATTTGATAAGAGTAATTTTGAAAAGTATTATTATTTCACTTCCATTATTACTATAAGGTTATTATTTTACTTTCATCTTCCTATAATATGTATTTTTTTTTTTTTTTGTAAAATAACATATTTAGTATTATAGTATATGGATATCGCCCCTACAATCTAAAATTTCTGGCTCCGCCACCGACTCCATATAAATTTTCTCTTTTTTTGTTTGTTTTAGTCTTTGTAAATATAGAATCTTTTGTTTTGGTTCACGGTGTTTCCTGGTTTAGCTAGGCCATGACTAAAAACTGCTAAAATTTCTTTGATATAACAAAACTATGATGTAATTATTTGCAGTGTTTGTGAACGGGAAATTCTGTAAAGACCCCGCGCTAGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCTAACGCACTAGGCTCTCAAGTAACTCAAGTCACTGCAGACCAACTATTTGGACTCAACACACTTGGGATATCTTTGGCTCGCGTTGATTTTGAGCCTAAGGGTTTAAACCCACCTCACATTCACCCTAGAGGAACGGAAATCCTTATAGTACTTGAATGTGGGATATGGCAACGCTGTTGCTATCGCTGGACTTAG
Protein sequence

>MS.gene70060.t1

MKIIYFLVSILALASSVAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHVEAGNASNALGSQVTQVTADQLFGLNTLGISLARVDFEPKGLNPPHIHPRGTEILIVLECGIWQRCCYRWT