AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70164

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70164.t1 MTR_8g094980 92.523 107 5 2 1 104 1 107 8.45e-60 178
MS.gene70164.t1 MTR_3g097120 74.038 104 23 2 1 104 7 106 1.07e-42 135
MS.gene70164.t1 MTR_7g118010 40.698 86 42 1 3 88 23 99 6.34e-19 75.1
MS.gene70164.t1 MTR_3g092220 41.176 85 41 1 3 87 33 108 8.90e-18 72.8
MS.gene70164.t1 MTR_8g026730 37.647 85 44 1 3 87 27 102 9.50e-17 69.7
MS.gene70164.t1 MTR_4g124750 43.023 86 36 2 3 88 69 141 4.36e-14 64.3
MS.gene70164.t1 MTR_2g043960 39.535 86 41 1 3 88 76 150 1.30e-13 63.2
MS.gene70164.t1 MTR_4g072910 51.389 72 23 2 3 73 35 95 1.02e-12 59.3
MS.gene70164.t1 MTR_4g072300 42.857 77 32 2 2 77 25 90 1.06e-12 58.9
MS.gene70164.t1 MTR_4g072940 40.000 80 36 2 1 79 55 123 1.11e-12 59.7
MS.gene70164.t1 MTR_4g072220 41.250 80 35 2 1 79 24 92 1.23e-12 58.9
MS.gene70164.t1 MTR_4g072330 42.857 77 32 2 2 77 22 87 1.73e-12 58.5
MS.gene70164.t1 MTR_4g072890 41.026 78 34 2 1 77 24 90 1.97e-12 58.2
MS.gene70164.t1 MTR_7g405740 39.474 76 42 1 28 99 81 156 2.75e-12 59.7
MS.gene70164.t1 MTR_1g061570 46.377 69 24 2 3 71 19 74 2.99e-12 58.5
MS.gene70164.t1 MTR_4g072820 43.590 78 32 2 2 78 19 85 3.65e-12 57.4
MS.gene70164.t1 MTR_4g072240 41.558 77 33 2 2 77 25 90 5.00e-12 57.4
MS.gene70164.t1 MTR_3g098970 41.429 70 30 1 1 70 51 109 5.66e-12 58.5
MS.gene70164.t1 MTR_4g072250 41.772 79 33 2 1 78 24 90 6.64e-12 57.0
MS.gene70164.t1 MTR_4g072260 41.026 78 34 2 1 77 24 90 6.95e-12 57.0
MS.gene70164.t1 MTR_4g072500 43.836 73 29 2 2 73 24 85 1.15e-11 56.2
MS.gene70164.t1 MTR_3g084250 41.026 78 34 2 1 77 23 89 1.34e-11 56.2
MS.gene70164.t1 MTR_1g059770 40.000 80 37 2 6 85 52 120 2.05e-11 56.6
MS.gene70164.t1 MTR_8g096500 45.205 73 29 1 6 78 52 113 2.14e-11 57.4
MS.gene70164.t1 MTR_4g072640 44.444 72 28 2 3 73 25 85 2.21e-11 55.8
MS.gene70164.t1 MTR_4g072560 38.961 77 36 2 3 78 32 98 2.46e-11 55.8
MS.gene70164.t1 MTR_4g072870 43.836 73 29 2 2 73 25 86 2.56e-11 55.5
MS.gene70164.t1 MTR_5g008370 44.444 72 28 2 3 73 25 85 2.96e-11 56.6
MS.gene70164.t1 MTR_8g096440 45.205 73 29 1 6 78 48 109 3.33e-11 56.2
MS.gene70164.t1 MTR_4g072230 43.836 73 27 2 5 76 28 87 3.50e-11 55.1
MS.gene70164.t1 MTR_4g072660 40.541 74 32 2 1 73 18 80 4.51e-11 54.7
MS.gene70164.t1 MTR_8g096470 40.541 74 33 1 6 79 49 111 6.39e-11 55.1
MS.gene70164.t1 MTR_4g072880 39.744 78 35 2 1 77 24 90 6.87e-11 54.3
MS.gene70164.t1 MTR_4g072480 41.892 74 31 2 1 73 23 85 7.32e-11 54.3
MS.gene70164.t1 MTR_8g461420 43.836 73 29 2 1 72 23 84 7.72e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70164.t1 AT2G46690 37.363 91 49 1 3 93 24 106 9.46e-19 75.5
MS.gene70164.t1 AT4G00880 39.759 83 41 1 2 84 27 100 3.80e-16 68.6
MS.gene70164.t1 AT5G53590 41.772 79 40 2 3 81 46 118 6.85e-16 68.6
MS.gene70164.t1 AT3G61900 40.000 80 40 1 3 82 30 101 1.13e-14 65.5
MS.gene70164.t1 AT5G20810 46.479 71 27 1 3 73 79 138 1.71e-12 60.5
MS.gene70164.t1 AT3G43120 43.421 76 32 1 3 78 79 143 3.06e-12 59.7
MS.gene70164.t1 AT5G20810 46.479 71 27 1 3 73 79 138 4.31e-12 59.7
MS.gene70164.t1 AT4G34780 40.000 80 39 2 1 79 26 97 7.18e-12 57.4
MS.gene70164.t1 AT4G34800 43.836 73 32 2 2 73 20 84 8.95e-12 56.6
MS.gene70164.t1 AT4G22620 37.333 75 39 1 3 77 79 145 9.52e-12 58.2
MS.gene70164.t1 AT4G34790 43.056 72 31 2 3 73 39 101 4.30e-11 55.5
MS.gene70164.t1 AT4G12410 34.667 75 41 1 3 77 76 142 4.77e-11 56.2

Find 23 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAAAGCTTATGAGATTTA+TGG 0.119621 4.1:+7725301 MS.gene70164:CDS
GGATGATAAAGATATGAAAT+AGG 0.226231 4.1:-7725259 None:intergenic
ATGATGGTGGTGATGGTGAT+AGG 0.327003 4.1:-7725410 None:intergenic
CTAAAAGATGAAGGTGAAGA+AGG 0.393733 4.1:+7725156 None:intergenic
GATGATAAAGATATGAAATA+GGG 0.436771 4.1:-7725258 None:intergenic
GTGGTGATGGTGATAGGGTG+TGG 0.458211 4.1:-7725404 None:intergenic
TCAAGAAGACGCTTGAAAAG+AGG 0.477121 4.1:-7725280 None:intergenic
TAAAAGATGAAGGTGAAGAA+GGG 0.520859 4.1:+7725157 None:intergenic
AACGGAACAAGGAAGCTTGA+GGG 0.534951 4.1:-7725341 None:intergenic
ACAAAGCATGAGGAATCAAA+TGG 0.539588 4.1:-7725438 None:intergenic
GGTGATGATGATGGTGGTGA+TGG 0.544241 4.1:-7725417 None:intergenic
GAAGAACACTCTCAAGAAGA+TGG 0.549372 4.1:+7725208 MS.gene70164:CDS
AGATGAAGGTGAAGAAGGGA+TGG 0.553458 4.1:+7725161 None:intergenic
TGATGGTGGTGATGGTGATA+GGG 0.561787 4.1:-7725409 None:intergenic
GAATCAAATGGTGATGATGA+TGG 0.574122 4.1:-7725426 None:intergenic
AGAAAATCATCAACGGAACA+AGG 0.574342 4.1:-7725352 None:intergenic
CAACGGAACAAGGAAGCTTG+AGG 0.591378 4.1:-7725342 None:intergenic
ATTTATGGCTACAACACTGA+TGG 0.615584 4.1:+7725316 MS.gene70164:CDS
ACGGAACAAGGAAGCTTGAG+GGG 0.635480 4.1:-7725340 None:intergenic
GGATGGCTAGCAGTTCAAGT+AGG 0.650962 4.1:+7725178 MS.gene70164:CDS
GAATTGAAATACAAAGCATG+AGG 0.674168 4.1:-7725448 None:intergenic
GAGGTGAAGAAAATCATCAA+CGG 0.680994 4.1:-7725359 None:intergenic
TCAAATGGTGATGATGATGG+TGG 0.690349 4.1:-7725423 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATGATAAAGATATGAAATA+GGG - chr4.1:7725261-7725280 None:intergenic 20.0%
! GATAAAGCTTATGAGATTTA+TGG + chr4.1:7725301-7725320 MS.gene70164:CDS 25.0%
! GGATGATAAAGATATGAAAT+AGG - chr4.1:7725262-7725281 None:intergenic 25.0%
GAATTGAAATACAAAGCATG+AGG - chr4.1:7725451-7725470 None:intergenic 30.0%
ACAAAGCATGAGGAATCAAA+TGG - chr4.1:7725441-7725460 None:intergenic 35.0%
AGAAAATCATCAACGGAACA+AGG - chr4.1:7725355-7725374 None:intergenic 35.0%
ATTTATGGCTACAACACTGA+TGG + chr4.1:7725316-7725335 MS.gene70164:CDS 35.0%
GAGGTGAAGAAAATCATCAA+CGG - chr4.1:7725362-7725381 None:intergenic 35.0%
! GAATCAAATGGTGATGATGA+TGG - chr4.1:7725429-7725448 None:intergenic 35.0%
!!! ATTCTTTTTCAACTCGCCAA+CGG - chr4.1:7725384-7725403 None:intergenic 35.0%
GAAGAACACTCTCAAGAAGA+TGG + chr4.1:7725208-7725227 MS.gene70164:CDS 40.0%
TCAAGAAGACGCTTGAAAAG+AGG - chr4.1:7725283-7725302 None:intergenic 40.0%
! ATGATTTTCTTCACCTCCGT+TGG + chr4.1:7725365-7725384 MS.gene70164:CDS 40.0%
!! TCAAATGGTGATGATGATGG+TGG - chr4.1:7725426-7725445 None:intergenic 40.0%
AACGGAACAAGGAAGCTTGA+GGG - chr4.1:7725344-7725363 None:intergenic 45.0%
!! ATGATGGTGGTGATGGTGAT+AGG - chr4.1:7725413-7725432 None:intergenic 45.0%
!! TGATGGTGGTGATGGTGATA+GGG - chr4.1:7725412-7725431 None:intergenic 45.0%
ACGGAACAAGGAAGCTTGAG+GGG - chr4.1:7725343-7725362 None:intergenic 50.0%
CAACGGAACAAGGAAGCTTG+AGG - chr4.1:7725345-7725364 None:intergenic 50.0%
GGATGGCTAGCAGTTCAAGT+AGG + chr4.1:7725178-7725197 MS.gene70164:CDS 50.0%
!! GGTGATGATGATGGTGGTGA+TGG - chr4.1:7725420-7725439 None:intergenic 50.0%
!!! CTTTTTCAACTCGCCAACGG+AGG - chr4.1:7725381-7725400 None:intergenic 50.0%
!! GTGGTGATGGTGATAGGGTG+TGG - chr4.1:7725407-7725426 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 7725163 7725477 7725163 ID=MS.gene70164
chr4.1 mRNA 7725163 7725477 7725163 ID=MS.gene70164.t1;Parent=MS.gene70164
chr4.1 exon 7725163 7725477 7725163 ID=MS.gene70164.t1.exon1;Parent=MS.gene70164.t1
chr4.1 CDS 7725163 7725477 7725163 ID=cds.MS.gene70164.t1;Parent=MS.gene70164.t1
Gene Sequence

>MS.gene70164

ATGAAGGTGAAGAAGGGATGGCTAGCAGTTCAAGTAGGTTCAGAAGAAGAACACTCTCAAGAAGATGGTGTAAGTGATTCTCAAAGATTTGTCATCCCTATTTCATATCTTTATCATCCTCTTTTCAAGCGTCTTCTTGATAAAGCTTATGAGATTTATGGCTACAACACTGATGGTCCCCTCAAGCTTCCTTGTTCCGTTGATGATTTTCTTCACCTCCGTTGGCGAGTTGAAAAAGAATCCACACCCTATCACCATCACCACCATCATCATCACCATTTGATTCCTCATGCTTTGTATTTCAATTCTTGTTGA
Protein sequence

>MS.gene70164.t1

MKVKKGWLAVQVGSEEEHSQEDGVSDSQRFVIPISYLYHPLFKRLLDKAYEIYGYNTDGPLKLPCSVDDFLHLRWRVEKESTPYHHHHHHHHHLIPHALYFNSC