AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059717


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059717.t1 AT2G21970 67.089 79 26 0 1 79 114 192 9.70e-32 110

Find 30 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTTGGGTGCGGTTACTA+TGG 0.311981 4.2:-10330495 MS.gene059717:CDS
GAATTCGGTGTTCAGGAAAA+TGG 0.321728 4.2:-10330549 MS.gene059717:CDS
GAGGCGGGTGGGATTTGTTT+GGG 0.354432 4.2:-10330509 MS.gene059717:CDS
GGAGGCGGGTGGGATTTGTT+TGG 0.366265 4.2:-10330510 MS.gene059717:CDS
GGAGTTGGTGACGGGGAATT+CGG 0.411121 4.2:-10330564 MS.gene059717:CDS
TTCAGGAAAATGGATATTGC+AGG 0.423134 4.2:-10330539 MS.gene059717:CDS
TGACGGGGAATTCGGTGTTC+AGG 0.428337 4.2:-10330556 MS.gene059717:CDS
ATCAATTAGCGAATCAATGA+AGG 0.432023 4.2:+10330403 None:intergenic
CTATGGCGGCAATGTTTGCT+TGG 0.440446 4.2:-10330478 MS.gene059717:CDS
GTGACGGTGGAGTTGGTGAC+GGG 0.448839 4.2:-10330572 MS.gene059717:CDS
CTAATTGATCAAATCGTTGA+CGG 0.458559 4.2:-10330389 MS.gene059717:CDS
AATGGATATTGCAGGGATAA+CGG 0.508574 4.2:-10330531 MS.gene059717:CDS
TATTGCAGGGATAACGGAGG+CGG 0.525078 4.2:-10330525 MS.gene059717:CDS
GCAGATGGTGTTTGCAGCGA+CGG 0.525390 4.2:-10330594 None:intergenic
TTTGGGTGCGGTTACTATGG+CGG 0.548728 4.2:-10330492 MS.gene059717:CDS
GGGTGGGATTTGTTTGGGTG+CGG 0.551031 4.2:-10330504 MS.gene059717:CDS
CAGGGATAACGGAGGCGGGT+GGG 0.551178 4.2:-10330520 MS.gene059717:CDS
GCAGGGATAACGGAGGCGGG+TGG 0.556957 4.2:-10330521 MS.gene059717:CDS
GGTGACGGTGGAGTTGGTGA+CGG 0.559321 4.2:-10330573 MS.gene059717:CDS
ATTGCAGGGATAACGGAGGC+GGG 0.570947 4.2:-10330524 MS.gene059717:CDS
TGACGGTGGAGTTGGTGACG+GGG 0.573283 4.2:-10330571 MS.gene059717:CDS
AGCGACGGTGACGGTGGAGT+TGG 0.587633 4.2:-10330579 MS.gene059717:CDS
TGTGAAGATTCGATCCACTC+TGG 0.602920 4.2:+10330439 None:intergenic
GGTGTTTGCAGCGACGGTGA+CGG 0.625726 4.2:-10330588 MS.gene059717:CDS
CTCAAGTGCTCGTACCAGAG+TGG 0.631041 4.2:-10330453 MS.gene059717:CDS
TTGTTCTATGATGATAGCGA+GGG 0.632266 4.2:-10330365 MS.gene059717:CDS
TTTGTTCTATGATGATAGCG+AGG 0.632796 4.2:-10330366 MS.gene059717:CDS
TCAGGAAAATGGATATTGCA+GGG 0.643208 4.2:-10330538 MS.gene059717:CDS
GTTTGCAGCGACGGTGACGG+TGG 0.675734 4.2:-10330585 MS.gene059717:CDS
GGATATTGCAGGGATAACGG+AGG 0.721943 4.2:-10330528 MS.gene059717:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATCAATTAGCGAATCAATGA+AGG + chr4.2:10330545-10330564 None:intergenic 30.0%
CTAATTGATCAAATCGTTGA+CGG - chr4.2:10330556-10330575 MS.gene059717:CDS 30.0%
AATGGATATTGCAGGGATAA+CGG - chr4.2:10330414-10330433 MS.gene059717:CDS 35.0%
TCAGGAAAATGGATATTGCA+GGG - chr4.2:10330407-10330426 MS.gene059717:CDS 35.0%
TTCAGGAAAATGGATATTGC+AGG - chr4.2:10330406-10330425 MS.gene059717:CDS 35.0%
! TTGTTCTATGATGATAGCGA+GGG - chr4.2:10330580-10330599 MS.gene059717:CDS 35.0%
! TTTGTTCTATGATGATAGCG+AGG - chr4.2:10330579-10330598 MS.gene059717:CDS 35.0%
!! GAATTCGGTGTTCAGGAAAA+TGG - chr4.2:10330396-10330415 MS.gene059717:CDS 40.0%
TGTGAAGATTCGATCCACTC+TGG + chr4.2:10330509-10330528 None:intergenic 45.0%
TTGTTTGGGTGCGGTTACTA+TGG - chr4.2:10330450-10330469 MS.gene059717:CDS 45.0%
CTATGGCGGCAATGTTTGCT+TGG - chr4.2:10330467-10330486 MS.gene059717:CDS 50.0%
TTTGGGTGCGGTTACTATGG+CGG - chr4.2:10330453-10330472 MS.gene059717:CDS 50.0%
! GGATATTGCAGGGATAACGG+AGG - chr4.2:10330417-10330436 MS.gene059717:CDS 50.0%
!! TATTGCAGGGATAACGGAGG+CGG - chr4.2:10330420-10330439 MS.gene059717:CDS 50.0%
CTCAAGTGCTCGTACCAGAG+TGG - chr4.2:10330492-10330511 MS.gene059717:CDS 55.0%
! GAGGCGGGTGGGATTTGTTT+GGG - chr4.2:10330436-10330455 MS.gene059717:CDS 55.0%
! GGGTGGGATTTGTTTGGGTG+CGG - chr4.2:10330441-10330460 MS.gene059717:CDS 55.0%
!! ATTGCAGGGATAACGGAGGC+GGG - chr4.2:10330421-10330440 MS.gene059717:CDS 55.0%
!! GGAGTTGGTGACGGGGAATT+CGG - chr4.2:10330381-10330400 MS.gene059717:CDS 55.0%
!! TGACGGGGAATTCGGTGTTC+AGG - chr4.2:10330389-10330408 MS.gene059717:CDS 55.0%
GGTGTTTGCAGCGACGGTGA+CGG - chr4.2:10330357-10330376 MS.gene059717:CDS 60.0%
! GGAGGCGGGTGGGATTTGTT+TGG - chr4.2:10330435-10330454 MS.gene059717:CDS 60.0%
!! GGTGACGGTGGAGTTGGTGA+CGG - chr4.2:10330372-10330391 MS.gene059717:CDS 60.0%
!! GTGACGGTGGAGTTGGTGAC+GGG - chr4.2:10330373-10330392 MS.gene059717:CDS 60.0%
!! TGACGGTGGAGTTGGTGACG+GGG - chr4.2:10330374-10330393 MS.gene059717:CDS 60.0%
! AGCGACGGTGACGGTGGAGT+TGG - chr4.2:10330366-10330385 MS.gene059717:CDS 65.0%
! GTTTGCAGCGACGGTGACGG+TGG - chr4.2:10330360-10330379 MS.gene059717:CDS 65.0%
!! CAGGGATAACGGAGGCGGGT+GGG - chr4.2:10330425-10330444 MS.gene059717:CDS 65.0%
!! GCAGGGATAACGGAGGCGGG+TGG - chr4.2:10330424-10330443 MS.gene059717:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 10330355 10330612 10330355 ID=MS.gene059717
chr4.2 mRNA 10330355 10330612 10330355 ID=MS.gene059717.t1;Parent=MS.gene059717
chr4.2 exon 10330355 10330612 10330355 ID=MS.gene059717.t1.exon1;Parent=MS.gene059717.t1
chr4.2 CDS 10330355 10330612 10330355 ID=cds.MS.gene059717.t1;Parent=MS.gene059717.t1
Gene Sequence

>MS.gene059717

ATGGTGTTTGCAGCGACGGTGACGGTGGAGTTGGTGACGGGGAATTCGGTGTTCAGGAAAATGGATATTGCAGGGATAACGGAGGCGGGTGGGATTTGTTTGGGTGCGGTTACTATGGCGGCAATGTTTGCTTGGTTCTCAAGTGCTCGTACCAGAGTGGATCGAATCTTCACAGTCAGCTACAACTCCTTCATTGATTCGCTAATTGATCAAATCGTTGACGGTTTGTTCTATGATGATAGCGAGGGTGAACTCTGA

Protein sequence

>MS.gene059717.t1

MVFAATVTVELVTGNSVFRKMDIAGITEAGGICLGAVTMAAMFAWFSSARTRVDRIFTVSYNSFIDSLIDQIVDGLFYDDSEGEL