Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054291 | MS.gene70284 | 0.814775 | 1.30E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAAATTGTTTCGCACTTT+AGG | 0.147440 | 4.1:-9099829 | MS.gene70284:CDS |
| GTCGATACATCATGTAATTT+TGG | 0.156748 | 4.1:-9098645 | MS.gene70284:CDS |
| CTAAATTGTTTCGCACTTTA+GGG | 0.213403 | 4.1:-9099828 | MS.gene70284:CDS |
| GCGAAACAATTTAGAGAAAT+TGG | 0.223102 | 4.1:+9099837 | None:intergenic |
| TTTGCTTTGCCAACAAATTC+AGG | 0.235324 | 4.1:-9099231 | MS.gene70284:CDS |
| TTGGACGAGCAAGACGAAAA+TGG | 0.346696 | 4.1:-9098626 | MS.gene70284:CDS |
| TATGAAGTTCCTGAATTTGT+TGG | 0.349014 | 4.1:+9099222 | None:intergenic |
| CTCGTCATTCTTGTATCACT+TGG | 0.351510 | 4.1:+9098582 | None:intergenic |
| TTCGATGTCTGCTACATAAC+GGG | 0.408530 | 4.1:-9099995 | MS.gene70284:CDS |
| AAGACGAAAATGGTTCTCTT+AGG | 0.415834 | 4.1:-9098616 | MS.gene70284:CDS |
| AAGGAGCTCTGTGAGTATAT+TGG | 0.424145 | 4.1:+9099252 | None:intergenic |
| ATTCTTGTATCACTTGGCTT+AGG | 0.429283 | 4.1:+9098588 | None:intergenic |
| ACAGCGAGAGAGGGAGAAGA+TGG | 0.439544 | 4.1:+9100082 | None:intergenic |
| CCCAGGCCTCCAAGCAAGAA+TGG | 0.458355 | 4.1:-9098674 | MS.gene70284:intron |
| CGGTGACATACCTCCCTCTC+TGG | 0.463844 | 4.1:-9100047 | MS.gene70284:CDS |
| GACCGAATGCCATTCTTGCT+TGG | 0.466954 | 4.1:+9098665 | None:intergenic |
| CTCGCTGTCGTCGACGGCGA+CGG | 0.470721 | 4.1:-9100067 | MS.gene70284:CDS |
| CGAATGCCATTCTTGCTTGG+AGG | 0.475816 | 4.1:+9098668 | None:intergenic |
| CCCTCTCTCGCTGTCGTCGA+CGG | 0.496851 | 4.1:-9100073 | MS.gene70284:CDS |
| ATCGAAGATCTTGATTAAGA+TGG | 0.523630 | 4.1:+9100012 | None:intergenic |
| TTCAGGAACTTCATATCTGA+AGG | 0.531356 | 4.1:-9099214 | MS.gene70284:intron |
| CTTCGATGTCTGCTACATAA+CGG | 0.537089 | 4.1:-9099996 | MS.gene70284:CDS |
| CTCCAAGCAAGAATGGCATT+CGG | 0.537957 | 4.1:-9098667 | MS.gene70284:CDS |
| CAGCGAGAGAGGGAGAAGAT+GGG | 0.551778 | 4.1:+9100083 | None:intergenic |
| TGAATTTGTTGGCAAAGCAA+AGG | 0.554064 | 4.1:+9099233 | None:intergenic |
| GATCTTGATTAAGATGGCGT+TGG | 0.561232 | 4.1:+9100018 | None:intergenic |
| TTGCACAAACACCGTTGCCA+AGG | 0.572894 | 4.1:-9099027 | MS.gene70284:intron |
| GCCGTCGACGACAGCGAGAG+AGG | 0.575432 | 4.1:+9100072 | None:intergenic |
| GAGAGAGGGAGAAGATGGGT+CGG | 0.575819 | 4.1:+9100087 | None:intergenic |
| TTGGCGAGAGCCAGAGAGGG+AGG | 0.580307 | 4.1:+9100037 | None:intergenic |
| CCGTCGACGACAGCGAGAGA+GGG | 0.584626 | 4.1:+9100073 | None:intergenic |
| TAAATTGTTTCGCACTTTAG+GGG | 0.593968 | 4.1:-9099827 | MS.gene70284:intron |
| GGCGTTGGCGAGAGCCAGAG+AGG | 0.594380 | 4.1:+9100033 | None:intergenic |
| GCGTTGGCGAGAGCCAGAGA+GGG | 0.631493 | 4.1:+9100034 | None:intergenic |
| GCTGATTCAAACCTTGGCAA+CGG | 0.634575 | 4.1:+9099016 | None:intergenic |
| ATTGGCGTGTTTGATGAAGT+CGG | 0.679645 | 4.1:+9099855 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTAATTAATATGATAAC+AGG | - | chr4.1:9099796-9099815 | MS.gene70284:intron | 15.0% |
| !!! | ATACAATGTATTTTTGTAAT+CGG | - | chr4.1:9099119-9099138 | MS.gene70284:intron | 15.0% |
| !!! | GATTGTTGTATTATTATTTT+AGG | - | chr4.1:9098910-9098929 | MS.gene70284:intron | 15.0% |
| !!! | TTTTTTTTTTTTCACTTACA+TGG | + | chr4.1:9099229-9099248 | None:intergenic | 15.0% |
| !! | AAAAAACTATAAAACAGAAG+CGG | - | chr4.1:9099248-9099267 | MS.gene70284:CDS | 20.0% |
| !! | ATTTAGTAACAATTAACATC+AGG | + | chr4.1:9099396-9099415 | None:intergenic | 20.0% |
| !! | TATAATGTTACAAATACGAT+GGG | + | chr4.1:9099760-9099779 | None:intergenic | 20.0% |
| !!! | GTATTAGTTTGAAATTGAAA+GGG | + | chr4.1:9099176-9099195 | None:intergenic | 20.0% |
| !!! | TACTAATAATTTTGAAAGCT+GGG | + | chr4.1:9099579-9099598 | None:intergenic | 20.0% |
| !!! | TATTTTTCCTGTTTAGATAA+TGG | - | chr4.1:9099370-9099389 | MS.gene70284:intron | 20.0% |
| !!! | TTACTAATAATTTTGAAAGC+TGG | + | chr4.1:9099580-9099599 | None:intergenic | 20.0% |
| ! | ACTTGTATAAATAACTATGC+AGG | - | chr4.1:9099090-9099109 | MS.gene70284:intron | 25.0% |
| ! | AGTAAGTTAGAGACATTTAT+TGG | + | chr4.1:9099073-9099092 | None:intergenic | 25.0% |
| ! | ATAAAAGTAGAGAACAAAGA+AGG | + | chr4.1:9099508-9099527 | None:intergenic | 25.0% |
| ! | ATTGTTACTAAATATTCTGC+AGG | - | chr4.1:9099403-9099422 | MS.gene70284:intron | 25.0% |
| ! | CATTCATTATTCCACTATTT+AGG | - | chr4.1:9098794-9098813 | MS.gene70284:intron | 25.0% |
| ! | CGATGAAGAAAGTAAATAAA+TGG | + | chr4.1:9098732-9098751 | None:intergenic | 25.0% |
| ! | CTATAATGTTACAAATACGA+TGG | + | chr4.1:9099761-9099780 | None:intergenic | 25.0% |
| ! | TAACAACAACAATAACAAAC+TGG | - | chr4.1:9099021-9099040 | MS.gene70284:intron | 25.0% |
| !! | GGTATTAGTTTGAAATTGAA+AGG | + | chr4.1:9099177-9099196 | None:intergenic | 25.0% |
| !! | TATCTGAAACACTTATTCAA+AGG | + | chr4.1:9099704-9099723 | None:intergenic | 25.0% |
| !!! | AATGTATTTTTGTAATCGGA+AGG | - | chr4.1:9099123-9099142 | MS.gene70284:intron | 25.0% |
| ACGTTAGTTAGTTAGTTAGT+TGG | + | chr4.1:9098945-9098964 | None:intergenic | 30.0% | |
| AGAGATAACACCAACAATAA+CGG | + | chr4.1:9099348-9099367 | None:intergenic | 30.0% | |
| ATCGAAGATCTTGATTAAGA+TGG | + | chr4.1:9098674-9098693 | None:intergenic | 30.0% | |
| CTAAATTGTTTCGCACTTTA+GGG | - | chr4.1:9098855-9098874 | MS.gene70284:intron | 30.0% | |
| CTAACGTTCTTATTAGATGA+TGG | - | chr4.1:9098958-9098977 | MS.gene70284:intron | 30.0% | |
| GCAAACAAATATGAATGCTT+TGG | + | chr4.1:9098984-9099003 | None:intergenic | 30.0% | |
| GCGAAACAATTTAGAGAAAT+TGG | + | chr4.1:9098849-9098868 | None:intergenic | 30.0% | |
| GGCATTGTAATTTAAAAGTG+AGG | + | chr4.1:9099300-9099319 | None:intergenic | 30.0% | |
| GTATAAATAACTATGCAGGT+GGG | - | chr4.1:9099094-9099113 | MS.gene70284:intron | 30.0% | |
| TAAATTGTTTCGCACTTTAG+GGG | - | chr4.1:9098856-9098875 | MS.gene70284:intron | 30.0% | |
| TATAAATAACTATGCAGGTG+GGG | - | chr4.1:9099095-9099114 | MS.gene70284:intron | 30.0% | |
| TATGAAGTTCCTGAATTTGT+TGG | + | chr4.1:9099464-9099483 | None:intergenic | 30.0% | |
| TCTAAATTGTTTCGCACTTT+AGG | - | chr4.1:9098854-9098873 | MS.gene70284:intron | 30.0% | |
| TGATCAAGTAACCTAAATAG+TGG | + | chr4.1:9098808-9098827 | None:intergenic | 30.0% | |
| TGTATAAATAACTATGCAGG+TGG | - | chr4.1:9099093-9099112 | MS.gene70284:intron | 30.0% | |
| ! | GCATTCATATTTGTTTGCTA+AGG | - | chr4.1:9098986-9099005 | MS.gene70284:intron | 30.0% |
| ! | GTCGATACATCATGTAATTT+TGG | - | chr4.1:9100038-9100057 | MS.gene70284:CDS | 30.0% |
| ! | TTGTTATTGTTGTTGTTACC+TGG | + | chr4.1:9099019-9099038 | None:intergenic | 30.0% |
| AAAAGTGAGGTGCAAATGTA+AGG | + | chr4.1:9099287-9099306 | None:intergenic | 35.0% | |
| AAAGTGAGGTGCAAATGTAA+GGG | + | chr4.1:9099286-9099305 | None:intergenic | 35.0% | |
| AACTCAACATAATGTTGTCC+TGG | + | chr4.1:9099921-9099940 | None:intergenic | 35.0% | |
| AAGACGAAAATGGTTCTCTT+AGG | - | chr4.1:9100067-9100086 | MS.gene70284:CDS | 35.0% | |
| ACATGTATATCGTTGTGTGA+TGG | - | chr4.1:9099860-9099879 | MS.gene70284:CDS | 35.0% | |
| ACTCAACATAATGTTGTCCT+GGG | + | chr4.1:9099920-9099939 | None:intergenic | 35.0% | |
| ATTCTTGTATCACTTGGCTT+AGG | + | chr4.1:9100098-9100117 | None:intergenic | 35.0% | |
| CATGTATATCGTTGTGTGAT+GGG | - | chr4.1:9099861-9099880 | MS.gene70284:CDS | 35.0% | |
| TCCAATTTCGCAAGTTAAGT+TGG | - | chr4.1:9098888-9098907 | MS.gene70284:intron | 35.0% | |
| TTCAGGAACTTCATATCTGA+AGG | - | chr4.1:9099469-9099488 | MS.gene70284:intron | 35.0% | |
| TTTCTATCTCGTAACAAGTC+TGG | - | chr4.1:9099533-9099552 | MS.gene70284:intron | 35.0% | |
| TTTGCTTTGCCAACAAATTC+AGG | - | chr4.1:9099452-9099471 | MS.gene70284:intron | 35.0% | |
| ! | AATAGAGCTGATTCAAACCT+TGG | + | chr4.1:9099676-9099695 | None:intergenic | 35.0% |
| ! | ATTGTTGTTGTTACCTGGAT+AGG | + | chr4.1:9099014-9099033 | None:intergenic | 35.0% |
| ! | TTGTTGTTGTTACCTGGATA+GGG | + | chr4.1:9099013-9099032 | None:intergenic | 35.0% |
| ! | TTTTTGTAATCGGAAGGAGA+AGG | - | chr4.1:9099129-9099148 | MS.gene70284:intron | 35.0% |
| !! | TCCAACTTAACTTGCGAAAT+TGG | + | chr4.1:9098892-9098911 | None:intergenic | 35.0% |
| !! | TGAATTTGTTGGCAAAGCAA+AGG | + | chr4.1:9099453-9099472 | None:intergenic | 35.0% |
| AACATAATGTTGTCCTGGGA+TGG | + | chr4.1:9099916-9099935 | None:intergenic | 40.0% | |
| AAGGAGCTCTGTGAGTATAT+TGG | + | chr4.1:9099434-9099453 | None:intergenic | 40.0% | |
| ATTGGCGTGTTTGATGAAGT+CGG | + | chr4.1:9098831-9098850 | None:intergenic | 40.0% | |
| CAACAATAACAAACTGGCTG+TGG | - | chr4.1:9099027-9099046 | MS.gene70284:intron | 40.0% | |
| CATCAGGCCATTATCTAAAC+AGG | + | chr4.1:9099380-9099399 | None:intergenic | 40.0% | |
| CTTCGATGTCTGCTACATAA+CGG | - | chr4.1:9098687-9098706 | MS.gene70284:intron | 40.0% | |
| GATCTTGATTAAGATGGCGT+TGG | + | chr4.1:9098668-9098687 | None:intergenic | 40.0% | |
| GTAGAGAACAAAGAAGGAGA+TGG | + | chr4.1:9099502-9099521 | None:intergenic | 40.0% | |
| TGATTGATATCGTACAACCC+AGG | - | chr4.1:9099992-9100011 | MS.gene70284:CDS | 40.0% | |
| TGTTAAATAGAAGCCATCCC+AGG | - | chr4.1:9099900-9099919 | MS.gene70284:intron | 40.0% | |
| TTCGATGTCTGCTACATAAC+GGG | - | chr4.1:9098688-9098707 | MS.gene70284:intron | 40.0% | |
| ! | ATATCTTGCTATGTGATGCC+TGG | - | chr4.1:9099966-9099985 | MS.gene70284:intron | 40.0% |
| ! | TATCTTGCTATGTGATGCCT+GGG | - | chr4.1:9099967-9099986 | MS.gene70284:intron | 40.0% |
| ! | TGCAAATGTAAGGGCATGTT+TGG | + | chr4.1:9099277-9099296 | None:intergenic | 40.0% |
| !! | AGAAGAATGAGGAAGGTGTT+GGG | + | chr4.1:9099321-9099340 | None:intergenic | 40.0% |
| CAACACCTTCCTCATTCTTC+TGG | - | chr4.1:9099320-9099339 | MS.gene70284:intron | 45.0% | |
| CTCCAAGCAAGAATGGCATT+CGG | - | chr4.1:9100016-9100035 | MS.gene70284:CDS | 45.0% | |
| GTACGATATCAATCACACCC+AGG | + | chr4.1:9099987-9100006 | None:intergenic | 45.0% | |
| TAAGACCCTGCAGGTGAAAA+CGG | + | chr4.1:9099630-9099649 | None:intergenic | 45.0% | |
| TTGGACGAGCAAGACGAAAA+TGG | - | chr4.1:9100057-9100076 | MS.gene70284:CDS | 45.0% | |
| ! | AATCTCCGTTTTCACCTGCA+GGG | - | chr4.1:9099622-9099641 | MS.gene70284:intron | 45.0% |
| ! | CAGAAGAATGAGGAAGGTGT+TGG | + | chr4.1:9099322-9099341 | None:intergenic | 45.0% |
| ! | GCTGATTCAAACCTTGGCAA+CGG | + | chr4.1:9099670-9099689 | None:intergenic | 45.0% |
| ! | TAATCTCCGTTTTCACCTGC+AGG | - | chr4.1:9099621-9099640 | MS.gene70284:intron | 45.0% |
| !! | CAATGTCTTTAAGACCCTGC+AGG | + | chr4.1:9099639-9099658 | None:intergenic | 45.0% |
| !! | AAAATTAAAAAAAAAAAACA+TGG | + | chr4.1:9099198-9099217 | None:intergenic | 5.0% |
| ATAACGGCGCCAGAAGAATG+AGG | + | chr4.1:9099332-9099351 | None:intergenic | 50.0% | |
| CGAATGCCATTCTTGCTTGG+AGG | + | chr4.1:9100018-9100037 | None:intergenic | 50.0% | |
| CTGGCTGTCATCATCATCTC+TGG | - | chr4.1:9099552-9099571 | MS.gene70284:intron | 50.0% | |
| GACCGAATGCCATTCTTGCT+TGG | + | chr4.1:9100021-9100040 | None:intergenic | 50.0% | |
| TCTTCTGGCGCCGTTATTGT+TGG | - | chr4.1:9099335-9099354 | MS.gene70284:intron | 50.0% | |
| TTGCACAAACACCGTTGCCA+AGG | - | chr4.1:9099656-9099675 | MS.gene70284:intron | 50.0% | |
| ! | GTGCTATTAGTGCCTTCGAG+TGG | - | chr4.1:9099726-9099745 | MS.gene70284:intron | 50.0% |
| ! | GTTTGCTAAGGTCCCTATCC+AGG | - | chr4.1:9098998-9099017 | MS.gene70284:intron | 50.0% |
| ACAGCGAGAGAGGGAGAAGA+TGG | + | chr4.1:9098604-9098623 | None:intergenic | 55.0% | |
| AGTGCCTTCGAGTGGCAGAA+CGG | - | chr4.1:9099734-9099753 | MS.gene70284:intron | 55.0% | |
| CAGCGAGAGAGGGAGAAGAT+GGG | + | chr4.1:9098603-9098622 | None:intergenic | 55.0% | |
| CCATTCTTGCTTGGAGGCCT+GGG | + | chr4.1:9100012-9100031 | None:intergenic | 55.0% | |
| GAGAGAGGGAGAAGATGGGT+CGG | + | chr4.1:9098599-9098618 | None:intergenic | 55.0% | |
| CCCAGGCCTCCAAGCAAGAA+TGG | - | chr4.1:9100009-9100028 | MS.gene70284:CDS | 60.0% | |
| CGGCGCCAGAAGAATGAGGA+AGG | + | chr4.1:9099328-9099347 | None:intergenic | 60.0% | |
| CGGTGACATACCTCCCTCTC+TGG | - | chr4.1:9098636-9098655 | MS.gene70284:CDS | 60.0% | |
| GCCATTCTTGCTTGGAGGCC+TGG | + | chr4.1:9100013-9100032 | None:intergenic | 60.0% | |
| CCCTCTCTCGCTGTCGTCGA+CGG | - | chr4.1:9098610-9098629 | MS.gene70284:CDS | 65.0% | |
| CCGTCGACGACAGCGAGAGA+GGG | + | chr4.1:9098613-9098632 | None:intergenic | 65.0% | |
| TGGGCCGTTCTGCCACTCGA+AGG | + | chr4.1:9099741-9099760 | None:intergenic | 65.0% | |
| TTGGCGAGAGCCAGAGAGGG+AGG | + | chr4.1:9098649-9098668 | None:intergenic | 65.0% | |
| ! | GCGTTGGCGAGAGCCAGAGA+GGG | + | chr4.1:9098652-9098671 | None:intergenic | 65.0% |
| CTCGCTGTCGTCGACGGCGA+CGG | - | chr4.1:9098616-9098635 | MS.gene70284:CDS | 70.0% | |
| GCCGTCGACGACAGCGAGAG+AGG | + | chr4.1:9098614-9098633 | None:intergenic | 70.0% | |
| !! | GGCGTTGGCGAGAGCCAGAG+AGG | + | chr4.1:9098653-9098672 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 9098586 | 9100119 | 9098586 | ID=MS.gene70284 |
| chr4.1 | mRNA | 9098586 | 9100119 | 9098586 | ID=MS.gene70284.t1;Parent=MS.gene70284 |
| chr4.1 | exon | 9099990 | 9100119 | 9099990 | ID=MS.gene70284.t1.exon1;Parent=MS.gene70284.t1 |
| chr4.1 | CDS | 9099990 | 9100119 | 9099990 | ID=cds.MS.gene70284.t1;Parent=MS.gene70284.t1 |
| chr4.1 | exon | 9099828 | 9099889 | 9099828 | ID=MS.gene70284.t1.exon2;Parent=MS.gene70284.t1 |
| chr4.1 | CDS | 9099828 | 9099889 | 9099828 | ID=cds.MS.gene70284.t1;Parent=MS.gene70284.t1 |
| chr4.1 | exon | 9099555 | 9099593 | 9099555 | ID=MS.gene70284.t1.exon3;Parent=MS.gene70284.t1 |
| chr4.1 | CDS | 9099555 | 9099593 | 9099555 | ID=cds.MS.gene70284.t1;Parent=MS.gene70284.t1 |
| chr4.1 | exon | 9099215 | 9099280 | 9099215 | ID=MS.gene70284.t1.exon4;Parent=MS.gene70284.t1 |
| chr4.1 | CDS | 9099215 | 9099280 | 9099215 | ID=cds.MS.gene70284.t1;Parent=MS.gene70284.t1 |
| chr4.1 | exon | 9099028 | 9099062 | 9099028 | ID=MS.gene70284.t1.exon5;Parent=MS.gene70284.t1 |
| chr4.1 | CDS | 9099028 | 9099062 | 9099028 | ID=cds.MS.gene70284.t1;Parent=MS.gene70284.t1 |
| chr4.1 | exon | 9098586 | 9098691 | 9098586 | ID=MS.gene70284.t1.exon6;Parent=MS.gene70284.t1 |
| chr4.1 | CDS | 9098586 | 9098691 | 9098586 | ID=cds.MS.gene70284.t1;Parent=MS.gene70284.t1 |
| Gene Sequence |
| Protein sequence |
