Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70377.t1 | XP_013457200.1 | 99.5 | 384 | 2 | 0 | 1 | 384 | 1 | 384 | 1.60E-223 | 785 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70377.t1 | Q66KH2 | 49.0 | 388 | 188 | 4 | 3 | 382 | 2 | 387 | 2.8e-104 | 380.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70377.t1 | A0A072UPC6 | 99.5 | 384 | 2 | 0 | 1 | 384 | 1 | 384 | 1.1e-223 | 785.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049180 | MS.gene70377 | 0.828471 | 8.83E-55 | -1.69E-46 |
| MS.gene050883 | MS.gene70377 | 0.80498 | 1.66E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70377.t1 | MTR_4g094255 | 99.479 | 384 | 2 | 0 | 1 | 384 | 1 | 384 | 0.0 | 795 |
| MS.gene70377.t1 | MTR_4g094255 | 99.322 | 295 | 2 | 0 | 90 | 384 | 1 | 295 | 0.0 | 614 |
| MS.gene70377.t1 | MTR_5g062720 | 68.052 | 385 | 121 | 1 | 1 | 383 | 1 | 385 | 0.0 | 591 |
| MS.gene70377.t1 | MTR_5g062720 | 67.987 | 303 | 97 | 0 | 1 | 303 | 1 | 303 | 9.89e-166 | 466 |
| MS.gene70377.t1 | MTR_3g081480 | 35.890 | 365 | 201 | 8 | 8 | 368 | 7 | 342 | 9.06e-73 | 231 |
| MS.gene70377.t1 | MTR_2g083260 | 25.503 | 298 | 185 | 10 | 104 | 382 | 199 | 478 | 4.10e-18 | 86.3 |
| MS.gene70377.t1 | MTR_2g083260 | 33.333 | 108 | 71 | 1 | 6 | 112 | 5 | 112 | 4.80e-13 | 70.9 |
| MS.gene70377.t1 | MTR_4g080800 | 33.166 | 199 | 102 | 8 | 202 | 382 | 290 | 475 | 8.01e-17 | 82.0 |
| MS.gene70377.t1 | MTR_4g080800 | 33.645 | 107 | 70 | 1 | 6 | 111 | 5 | 111 | 2.54e-13 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70377.t1 | AT1G36050 | 70.390 | 385 | 112 | 1 | 1 | 383 | 1 | 385 | 0.0 | 592 |
| MS.gene70377.t1 | AT1G36050 | 70.419 | 382 | 111 | 1 | 1 | 380 | 1 | 382 | 0.0 | 587 |
| MS.gene70377.t1 | AT1G22200 | 69.091 | 385 | 117 | 1 | 1 | 383 | 1 | 385 | 0.0 | 580 |
| MS.gene70377.t1 | AT1G22200 | 68.657 | 335 | 103 | 1 | 1 | 333 | 1 | 335 | 7.63e-179 | 500 |
| MS.gene70377.t1 | AT3G22290 | 36.483 | 381 | 201 | 10 | 4 | 377 | 3 | 349 | 1.56e-71 | 228 |
| MS.gene70377.t1 | AT3G22290 | 36.483 | 381 | 201 | 10 | 4 | 377 | 3 | 349 | 1.56e-71 | 228 |
| MS.gene70377.t1 | AT4G27080 | 30.631 | 222 | 122 | 9 | 179 | 382 | 271 | 478 | 9.90e-19 | 88.2 |
| MS.gene70377.t1 | AT4G27080 | 31.818 | 110 | 74 | 1 | 6 | 114 | 5 | 114 | 2.36e-13 | 71.6 |
| MS.gene70377.t1 | AT4G27080 | 30.631 | 222 | 122 | 9 | 179 | 382 | 323 | 530 | 1.13e-18 | 88.2 |
| MS.gene70377.t1 | AT4G27080 | 32.039 | 103 | 69 | 1 | 13 | 114 | 64 | 166 | 3.47e-11 | 65.1 |
| MS.gene70377.t1 | AT1G50950 | 37.037 | 108 | 67 | 1 | 6 | 112 | 5 | 112 | 7.22e-15 | 76.3 |
| MS.gene70377.t1 | AT1G50950 | 25.887 | 282 | 166 | 10 | 124 | 382 | 226 | 487 | 1.87e-11 | 65.9 |
| MS.gene70377.t1 | AT1G50950 | 37.037 | 108 | 67 | 1 | 6 | 112 | 5 | 112 | 7.47e-15 | 76.3 |
| MS.gene70377.t1 | AT1G50950 | 25.887 | 282 | 166 | 10 | 124 | 382 | 221 | 482 | 2.04e-11 | 65.9 |
| MS.gene70377.t1 | AT3G20560 | 28.856 | 201 | 111 | 7 | 202 | 382 | 293 | 481 | 2.38e-14 | 74.7 |
| MS.gene70377.t1 | AT3G20560 | 35.185 | 108 | 69 | 1 | 6 | 112 | 5 | 112 | 1.01e-13 | 72.8 |
Find 88 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTACTCTTACCTTTAT+TGG | 0.086581 | 4.3:+20208696 | None:intergenic |
| GATGATTACATTGATGTTTA+AGG | 0.116866 | 4.3:+20213636 | None:intergenic |
| TCTTCAGTCAGCTATCTTTC+TGG | 0.129021 | 4.3:-20210934 | MS.gene70377:CDS |
| TAATGCTTTGCAGGATAAAA+TGG | 0.150023 | 4.3:-20209334 | MS.gene70377:intron |
| GGCTGATCTACTTGCTTTGC+AGG | 0.231010 | 4.3:-20210913 | MS.gene70377:CDS |
| CTTCAACAAGCTCCGCAATT+TGG | 0.259431 | 4.3:-20214722 | MS.gene70377:CDS |
| TACAAACCTTTATGAAATAT+TGG | 0.288463 | 4.3:+20209285 | None:intergenic |
| CAGATCAACAAATTAAGTTT+TGG | 0.299273 | 4.3:-20210641 | MS.gene70377:CDS |
| ACCAATATTTGTGCAATCAT+TGG | 0.300995 | 4.3:-20207853 | MS.gene70377:CDS |
| AAATATTGTGGTTCTTGTTT+TGG | 0.315971 | 4.3:-20212713 | MS.gene70377:CDS |
| GTGGATTCATCAATATATTA+TGG | 0.317761 | 4.3:-20207698 | MS.gene70377:CDS |
| TCCAATGATTGCACAAATAT+TGG | 0.332241 | 4.3:+20207852 | None:intergenic |
| AGAACTGGGTGCTGCAGTTC+CGG | 0.349349 | 4.3:-20208745 | MS.gene70377:CDS |
| GAACTGGGTGCTGCAGTTCC+GGG | 0.369702 | 4.3:-20208744 | MS.gene70377:CDS |
| TGTTACAGAGAGTAAGCTTT+TGG | 0.377097 | 4.3:-20213680 | MS.gene70377:CDS |
| GGGATGGGCACTGACAAATA+TGG | 0.383809 | 4.3:-20211346 | MS.gene70377:CDS |
| CTATGACATATCTCCAATAA+AGG | 0.384793 | 4.3:-20208709 | MS.gene70377:intron |
| GCATTTCAAGAGTTCAGAAC+TGG | 0.386769 | 4.3:-20208760 | MS.gene70377:CDS |
| AAACAAAGAATTGATGCTAA+TGG | 0.405935 | 4.3:-20213157 | MS.gene70377:CDS |
| GGATTGGTTAATCCTCTTGA+TGG | 0.406458 | 4.3:-20210605 | MS.gene70377:CDS |
| ATAGGTATATTCACAATTGC+GGG | 0.416093 | 4.3:-20207725 | MS.gene70377:intron |
| ATCAAGAAGAAGATGGAGAT+TGG | 0.425527 | 4.3:-20207665 | MS.gene70377:CDS |
| CAAAGTGATAGAAGCTAGAA+AGG | 0.432471 | 4.3:-20213134 | MS.gene70377:CDS |
| GGTGAAGGATGCAATATTCA+TGG | 0.439184 | 4.3:-20211014 | MS.gene70377:CDS |
| AACTTAATTTGTTGATCTGA+TGG | 0.440792 | 4.3:+20210645 | None:intergenic |
| TCGTGTTATATATTCAAATC+AGG | 0.444045 | 4.3:-20208881 | MS.gene70377:intron |
| AAATATGGATTTGATCGATC+AGG | 0.446849 | 4.3:-20211331 | MS.gene70377:intron |
| AAGAAGTTCGTGAGGCATAT+AGG | 0.446884 | 4.3:-20211374 | MS.gene70377:CDS |
| TCAAGAGGATTAACCAATCC+AGG | 0.448600 | 4.3:+20210608 | None:intergenic |
| CATGTACCAATATTTCATAA+AGG | 0.449326 | 4.3:-20209291 | MS.gene70377:intron |
| CCTGCCACCATGTTTCTGTA+AGG | 0.455242 | 4.3:+20212751 | None:intergenic |
| TTAGACACAATGGATATTAG+TGG | 0.459541 | 4.3:-20213320 | MS.gene70377:CDS |
| ACATGCCATGATCATTTCCT+TGG | 0.466746 | 4.3:+20209309 | None:intergenic |
| GAAGCTAGAAAGGAAGGAAT+TGG | 0.469298 | 4.3:-20213124 | MS.gene70377:CDS |
| ACAGTATACACGGACATTAG+AGG | 0.477413 | 4.3:-20208904 | MS.gene70377:CDS |
| TGGTCACCATTACCCTGGAT+TGG | 0.479452 | 4.3:-20210621 | MS.gene70377:CDS |
| GTGATAGAAGCTAGAAAGGA+AGG | 0.482180 | 4.3:-20213130 | MS.gene70377:CDS |
| AGATTGGAAAATATAGATGA+TGG | 0.489952 | 4.3:-20207649 | None:intergenic |
| ATTCACAATTGCGGGAATTG+TGG | 0.490506 | 4.3:-20207717 | MS.gene70377:CDS |
| GAAAACAATCAAGAAGAAGA+TGG | 0.504536 | 4.3:-20207672 | MS.gene70377:CDS |
| CTTGTTGAAGACTTTATCCA+TGG | 0.505504 | 4.3:+20214735 | None:intergenic |
| TTTCCCTATTGCAGATTGAA+AGG | 0.505700 | 4.3:-20212775 | MS.gene70377:intron |
| AATATTTGTGCAATCATTGG+AGG | 0.520187 | 4.3:-20207850 | MS.gene70377:intron |
| CTAATGTCCGTGTATACTGT+AGG | 0.520758 | 4.3:+20208907 | None:intergenic |
| GTTGCAGGTCAACTTTAAAG+AGG | 0.521061 | 4.3:-20207902 | MS.gene70377:intron |
| GATTGGTTAATCCTCTTGAT+GGG | 0.521240 | 4.3:-20210604 | MS.gene70377:intron |
| TTCTGAACTCTTGAAATGCT+CGG | 0.529904 | 4.3:+20208764 | None:intergenic |
| CTTGAAGTGAATAAAGTGGC+AGG | 0.531002 | 4.3:-20210987 | MS.gene70377:CDS |
| AATGCTTTGCAGGATAAAAT+GGG | 0.531280 | 4.3:-20209333 | MS.gene70377:intron |
| TCGTGAGGCATATAGGAAGA+AGG | 0.532464 | 4.3:-20211367 | MS.gene70377:CDS |
| TAGCTGACTGAAGAAAGCTT+TGG | 0.540463 | 4.3:+20210943 | None:intergenic |
| GGGTCATATCATACTTAGCT+CGG | 0.541362 | 4.3:+20214600 | None:intergenic |
| TACTTAGCTCGGAAATAAAG+AGG | 0.546697 | 4.3:+20214611 | None:intergenic |
| GATTAACCAATCCAGGGTAA+TGG | 0.548392 | 4.3:+20210615 | None:intergenic |
| AGGCATATAGGAAGAAGGGA+TGG | 0.551318 | 4.3:-20211362 | MS.gene70377:CDS |
| CCTTACAGAAACATGGTGGC+AGG | 0.551937 | 4.3:-20212751 | MS.gene70377:CDS |
| GAGGAAAAGCATGACGGCGG+CGG | 0.554845 | 4.3:+20214630 | None:intergenic |
| CATAGGTATATTCACAATTG+CGG | 0.556363 | 4.3:-20207726 | MS.gene70377:intron |
| TGGGTCCAAGGAAATGATCA+TGG | 0.561072 | 4.3:-20209314 | MS.gene70377:CDS |
| TATAACCGCACTCTCGCCGG+AGG | 0.565571 | 4.3:-20214670 | MS.gene70377:CDS |
| CTGCCACCATGTTTCTGTAA+GGG | 0.566564 | 4.3:+20212752 | None:intergenic |
| AAAGAAAATTACCCATCAAG+AGG | 0.569599 | 4.3:+20210593 | None:intergenic |
| TAATGTCCGTGTATACTGTA+GGG | 0.574539 | 4.3:+20208908 | None:intergenic |
| AGTATTGAACATCGAACAGC+AGG | 0.576281 | 4.3:+20213347 | None:intergenic |
| GGAAGGAATTGGCGCACCAA+AGG | 0.576811 | 4.3:-20213113 | MS.gene70377:intron |
| ACTCTTGAAATGCTCGGTCA+CGG | 0.579209 | 4.3:+20208770 | None:intergenic |
| GACGACGGTGACGACACCTC+CGG | 0.579378 | 4.3:+20214654 | None:intergenic |
| TTGGGTATGCATCCAAATTG+CGG | 0.580291 | 4.3:+20214710 | None:intergenic |
| CGTGAGGCATATAGGAAGAA+GGG | 0.582433 | 4.3:-20211366 | MS.gene70377:CDS |
| TCATAGAAGAAGAAAACTCC+CGG | 0.587428 | 4.3:+20208726 | None:intergenic |
| CATGACGGCGGCGGAGACGA+CGG | 0.590043 | 4.3:+20214639 | None:intergenic |
| ATCTCTTGAAGTGAATAAAG+TGG | 0.597114 | 4.3:-20210991 | MS.gene70377:CDS |
| TGCAGGATAAAATGGGTCCA+AGG | 0.598124 | 4.3:-20209326 | MS.gene70377:intron |
| CATTTCAAGAGTTCAGAACT+GGG | 0.599994 | 4.3:-20208759 | MS.gene70377:CDS |
| AATACTCAGTTTAGACACAA+TGG | 0.600279 | 4.3:-20213330 | MS.gene70377:CDS |
| CTTCACAATTATTACAACAG+TGG | 0.607704 | 4.3:+20211394 | None:intergenic |
| CAAAAGGTAAAGGATGAAGA+AGG | 0.608037 | 4.3:-20211035 | MS.gene70377:CDS |
| GTAAAGGATGAAGAAGGTGA+AGG | 0.610725 | 4.3:-20211029 | MS.gene70377:CDS |
| CAAGAGGATTAACCAATCCA+GGG | 0.627731 | 4.3:+20210609 | None:intergenic |
| CGACACCTCCGGCGAGAGTG+CGG | 0.635707 | 4.3:+20214665 | None:intergenic |
| GGCATATAGGAAGAAGGGAT+GGG | 0.636215 | 4.3:-20211361 | MS.gene70377:CDS |
| GAAAGGCCCTTACAGAAACA+TGG | 0.652386 | 4.3:-20212758 | MS.gene70377:CDS |
| GGTGGTCCCTACAGTATACA+CGG | 0.683793 | 4.3:-20208914 | MS.gene70377:CDS |
| AATAAAGAGGAAAAGCATGA+CGG | 0.687611 | 4.3:+20214624 | None:intergenic |
| TAATTGTGAAGAAGTTCGTG+AGG | 0.690238 | 4.3:-20211382 | MS.gene70377:CDS |
| CAATGGATATTAGTGGCGAG+CGG | 0.694408 | 4.3:-20213313 | MS.gene70377:CDS |
| AAAGAGGAAAAGCATGACGG+CGG | 0.739902 | 4.3:+20214627 | None:intergenic |
| AGGCCCTTACAGAAACATGG+TGG | 0.756459 | 4.3:-20212755 | MS.gene70377:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 20207652 | 20214755 | 20207652 | ID=MS.gene70377 |
| chr4.3 | mRNA | 20207652 | 20214755 | 20207652 | ID=MS.gene70377.t1;Parent=MS.gene70377 |
| chr4.3 | exon | 20214614 | 20214755 | 20214614 | ID=MS.gene70377.t1.exon1;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20214614 | 20214755 | 20214614 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20213645 | 20213718 | 20213645 | ID=MS.gene70377.t1.exon2;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20213645 | 20213718 | 20213645 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20213301 | 20213384 | 20213301 | ID=MS.gene70377.t1.exon3;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20213301 | 20213384 | 20213301 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20213114 | 20213194 | 20213114 | ID=MS.gene70377.t1.exon4;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20213114 | 20213194 | 20213114 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20212703 | 20212783 | 20212703 | ID=MS.gene70377.t1.exon5;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20212703 | 20212783 | 20212703 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20211332 | 20211424 | 20211332 | ID=MS.gene70377.t1.exon6;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20211332 | 20211424 | 20211332 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20210899 | 20211078 | 20210899 | ID=MS.gene70377.t1.exon7;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20210899 | 20211078 | 20210899 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20210605 | 20210672 | 20210605 | ID=MS.gene70377.t1.exon8;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20210605 | 20210672 | 20210605 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20209292 | 20209343 | 20209292 | ID=MS.gene70377.t1.exon9;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20209292 | 20209343 | 20209292 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20208882 | 20208935 | 20208882 | ID=MS.gene70377.t1.exon10;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20208882 | 20208935 | 20208882 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20208710 | 20208796 | 20208710 | ID=MS.gene70377.t1.exon11;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20208710 | 20208796 | 20208710 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20207851 | 20207917 | 20207851 | ID=MS.gene70377.t1.exon12;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20207851 | 20207917 | 20207851 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| chr4.3 | exon | 20207652 | 20207743 | 20207652 | ID=MS.gene70377.t1.exon13;Parent=MS.gene70377.t1 |
| chr4.3 | CDS | 20207652 | 20207743 | 20207652 | ID=cds.MS.gene70377.t1;Parent=MS.gene70377.t1 |
| Gene Sequence |
| Protein sequence |
