Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70607.t1 | XP_013461022.1 | 95.7 | 140 | 5 | 1 | 1 | 139 | 1 | 140 | 2.70E-72 | 281.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70607.t1 | I3T405 | 95.7 | 140 | 5 | 1 | 1 | 139 | 1 | 140 | 2.0e-72 | 281.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05548 | MS.gene70607 | 0.806489 | 8.02E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene70607 | 0.812925 | 3.32E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70607.t1 | MTR_3g078240 | 95.714 | 140 | 5 | 1 | 1 | 139 | 1 | 140 | 1.14e-98 | 280 |
| MS.gene70607.t1 | MTR_4g106650 | 50.413 | 121 | 34 | 5 | 18 | 121 | 21 | 132 | 6.90e-31 | 108 |
| MS.gene70607.t1 | MTR_6g007727 | 46.269 | 134 | 55 | 6 | 16 | 138 | 27 | 154 | 8.27e-28 | 100 |
| MS.gene70607.t1 | MTR_2g461190 | 45.333 | 75 | 35 | 3 | 50 | 124 | 66 | 134 | 3.15e-13 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70607.t1 | AT5G14895 | 37.419 | 155 | 75 | 7 | 1 | 135 | 11 | 163 | 6.65e-25 | 94.0 |
| MS.gene70607.t1 | AT3G48020 | 44.037 | 109 | 52 | 3 | 29 | 129 | 18 | 125 | 4.93e-22 | 85.5 |
| MS.gene70607.t1 | AT3G01430 | 35.256 | 156 | 60 | 7 | 17 | 136 | 27 | 177 | 6.00e-21 | 84.0 |
| MS.gene70607.t1 | AT5G62865 | 37.580 | 157 | 77 | 6 | 2 | 140 | 5 | 158 | 1.33e-20 | 82.8 |
Find 46 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAATGGAAATTGGAATTT+AGG | 0.187114 | 3.1:+64201323 | MS.gene70607:CDS |
| GAAGTCTGATTCGACGGATT+TGG | 0.249257 | 3.1:+64201284 | MS.gene70607:CDS |
| ATGATTCTCCTGACGGGTTT+CGG | 0.304930 | 3.1:+64201223 | MS.gene70607:CDS |
| ACCTTCTGCTTCCCATGCTT+CGG | 0.359990 | 3.1:+64200961 | MS.gene70607:CDS |
| GTCGTCGTTTCAATGGAAAT+TGG | 0.360494 | 3.1:+64201315 | MS.gene70607:CDS |
| CGTTTATTCGACGGTTCAAC+CGG | 0.363885 | 3.1:+64201103 | MS.gene70607:CDS |
| GGAAACCACCGCTCTTGTTC+CGG | 0.390683 | 3.1:-64201122 | None:intergenic |
| GAGTGGTCGGAAATCGTTGC+TGG | 0.429415 | 3.1:+64201066 | MS.gene70607:CDS |
| ACAGAACGTCGTCGTTTCAA+TGG | 0.436107 | 3.1:+64201308 | MS.gene70607:CDS |
| CCACCATGGTTCGGTGGTGG+TGG | 0.445372 | 3.1:-64200995 | None:intergenic |
| ATTTCGATGAGGGGCAGAAT+GGG | 0.456984 | 3.1:+64201190 | MS.gene70607:CDS |
| CACACGCTCCCACCATGGTT+CGG | 0.489564 | 3.1:-64201004 | None:intergenic |
| AGCGGTGGTTTCCGTCATGC+GGG | 0.490593 | 3.1:+64201132 | MS.gene70607:CDS |
| CTCCCACCATGGTTCGGTGG+TGG | 0.505296 | 3.1:-64200998 | None:intergenic |
| TCGAAAATGATTCTCCTGAC+GGG | 0.518747 | 3.1:+64201217 | MS.gene70607:CDS |
| CATGAAAATCCGCGAGTGGT+CGG | 0.522571 | 3.1:+64201053 | MS.gene70607:CDS |
| GACTTTGAAGTCTGATTCGA+CGG | 0.523532 | 3.1:+64201278 | MS.gene70607:CDS |
| AATTTCGATGAGGGGCAGAA+TGG | 0.524198 | 3.1:+64201189 | MS.gene70607:CDS |
| TTCGAAAATGATTCTCCTGA+CGG | 0.534748 | 3.1:+64201216 | MS.gene70607:CDS |
| GAGCGGTGGTTTCCGTCATG+CGG | 0.545646 | 3.1:+64201131 | MS.gene70607:CDS |
| GAGTGACGAGAACCGAAGCA+TGG | 0.562799 | 3.1:-64200973 | None:intergenic |
| TACTCTTTGAATTTCGATGA+GGG | 0.566198 | 3.1:+64201180 | MS.gene70607:CDS |
| AGTGACGAGAACCGAAGCAT+GGG | 0.577091 | 3.1:-64200972 | None:intergenic |
| CACCACCACCGAACCATGGT+GGG | 0.578901 | 3.1:+64200996 | MS.gene70607:CDS |
| ACGCTCCCACCATGGTTCGG+TGG | 0.592107 | 3.1:-64201001 | None:intergenic |
| GGTGGAAGACGTTTATTCGA+CGG | 0.599260 | 3.1:+64201094 | MS.gene70607:CDS |
| CGGAAATCGTTGCTGGACCA+CGG | 0.599286 | 3.1:+64201073 | MS.gene70607:CDS |
| ATTGATACTTCCCCGCATGA+CGG | 0.603381 | 3.1:-64201143 | None:intergenic |
| TTACTCTTTGAATTTCGATG+AGG | 0.616940 | 3.1:+64201179 | MS.gene70607:CDS |
| TTTCGATGAGGGGCAGAATG+GGG | 0.623061 | 3.1:+64201191 | MS.gene70607:CDS |
| CGAGCTTCGTCTGTATCACG+TGG | 0.623082 | 3.1:+64201027 | MS.gene70607:CDS |
| GTCGAATCAGACTTCAAAGT+CGG | 0.636138 | 3.1:-64201276 | None:intergenic |
| AAATTCAAAGAGTAACTCAA+AGG | 0.636783 | 3.1:-64201171 | None:intergenic |
| ACCGAAGCATGGGAAGCAGA+AGG | 0.639919 | 3.1:-64200962 | None:intergenic |
| CAACGATTTCCGACCACTCG+CGG | 0.640334 | 3.1:-64201062 | None:intergenic |
| ACTCTTTGAATTTCGATGAG+GGG | 0.647474 | 3.1:+64201181 | MS.gene70607:CDS |
| CCACCACCACCGAACCATGG+TGG | 0.647847 | 3.1:+64200995 | MS.gene70607:CDS |
| ACTCCACCACCACCGAACCA+TGG | 0.649404 | 3.1:+64200992 | MS.gene70607:CDS |
| TTCAGCTCGTTACGTTGCGG+CGG | 0.650823 | 3.1:+64201251 | MS.gene70607:CDS |
| GCTCGCACACGCTCCCACCA+TGG | 0.651664 | 3.1:-64201009 | None:intergenic |
| CGGTTCAACCGGAACAAGAG+CGG | 0.664702 | 3.1:+64201114 | MS.gene70607:CDS |
| TTCAACCGGAACAAGAGCGG+TGG | 0.667010 | 3.1:+64201117 | MS.gene70607:CDS |
| GCGGTGGTTTCCGTCATGCG+GGG | 0.669488 | 3.1:+64201133 | MS.gene70607:CDS |
| GATTCATGAAAATCCGCGAG+TGG | 0.686689 | 3.1:+64201049 | MS.gene70607:CDS |
| CGAATAAACGTCTTCCACCG+TGG | 0.729057 | 3.1:-64201090 | None:intergenic |
| AAATCGTTGCTGGACCACGG+TGG | 0.739447 | 3.1:+64201076 | MS.gene70607:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTCAAAGAGTAACTCAA+AGG | - | chr3.1:64201174-64201193 | None:intergenic | 25.0% |
| TACTCTTTGAATTTCGATGA+GGG | + | chr3.1:64201180-64201199 | MS.gene70607:CDS | 30.0% | |
| TTACTCTTTGAATTTCGATG+AGG | + | chr3.1:64201179-64201198 | MS.gene70607:CDS | 30.0% | |
| ACTCTTTGAATTTCGATGAG+GGG | + | chr3.1:64201181-64201200 | MS.gene70607:CDS | 35.0% | |
| TTCGAAAATGATTCTCCTGA+CGG | + | chr3.1:64201216-64201235 | MS.gene70607:CDS | 35.0% | |
| GTCGAATCAGACTTCAAAGT+CGG | - | chr3.1:64201279-64201298 | None:intergenic | 40.0% | |
| GTCGTCGTTTCAATGGAAAT+TGG | + | chr3.1:64201315-64201334 | MS.gene70607:CDS | 40.0% | |
| TCGAAAATGATTCTCCTGAC+GGG | + | chr3.1:64201217-64201236 | MS.gene70607:CDS | 40.0% | |
| !! | GACTTTGAAGTCTGATTCGA+CGG | + | chr3.1:64201278-64201297 | MS.gene70607:CDS | 40.0% |
| !! | TTTTTCAGCTCGTTACGTTG+CGG | + | chr3.1:64201248-64201267 | MS.gene70607:CDS | 40.0% |
| AATTTCGATGAGGGGCAGAA+TGG | + | chr3.1:64201189-64201208 | MS.gene70607:CDS | 45.0% | |
| ACAGAACGTCGTCGTTTCAA+TGG | + | chr3.1:64201308-64201327 | MS.gene70607:CDS | 45.0% | |
| ATTGATACTTCCCCGCATGA+CGG | - | chr3.1:64201146-64201165 | None:intergenic | 45.0% | |
| ATTTCGATGAGGGGCAGAAT+GGG | + | chr3.1:64201190-64201209 | MS.gene70607:CDS | 45.0% | |
| CGTTTATTCGACGGTTCAAC+CGG | + | chr3.1:64201103-64201122 | MS.gene70607:CDS | 45.0% | |
| GAAAAATTCCGAAACCCGTC+AGG | - | chr3.1:64201234-64201253 | None:intergenic | 45.0% | |
| GATTCATGAAAATCCGCGAG+TGG | + | chr3.1:64201049-64201068 | MS.gene70607:CDS | 45.0% | |
| GGTGGAAGACGTTTATTCGA+CGG | + | chr3.1:64201094-64201113 | MS.gene70607:CDS | 45.0% | |
| ! | GAAGTCTGATTCGACGGATT+TGG | + | chr3.1:64201284-64201303 | MS.gene70607:CDS | 45.0% |
| !! | ATGATTCTCCTGACGGGTTT+CGG | + | chr3.1:64201223-64201242 | MS.gene70607:CDS | 45.0% |
| ACCTTCTGCTTCCCATGCTT+CGG | + | chr3.1:64200961-64200980 | MS.gene70607:CDS | 50.0% | |
| AGTGACGAGAACCGAAGCAT+GGG | - | chr3.1:64200975-64200994 | None:intergenic | 50.0% | |
| CATGAAAATCCGCGAGTGGT+CGG | + | chr3.1:64201053-64201072 | MS.gene70607:CDS | 50.0% | |
| CGAATAAACGTCTTCCACCG+TGG | - | chr3.1:64201093-64201112 | None:intergenic | 50.0% | |
| TTTCGATGAGGGGCAGAATG+GGG | + | chr3.1:64201191-64201210 | MS.gene70607:CDS | 50.0% | |
| AAATCGTTGCTGGACCACGG+TGG | + | chr3.1:64201076-64201095 | MS.gene70607:CDS | 55.0% | |
| ACCGAAGCATGGGAAGCAGA+AGG | - | chr3.1:64200965-64200984 | None:intergenic | 55.0% | |
| CAACGATTTCCGACCACTCG+CGG | - | chr3.1:64201065-64201084 | None:intergenic | 55.0% | |
| CGAGCTTCGTCTGTATCACG+TGG | + | chr3.1:64201027-64201046 | MS.gene70607:CDS | 55.0% | |
| CGGAAATCGTTGCTGGACCA+CGG | + | chr3.1:64201073-64201092 | MS.gene70607:CDS | 55.0% | |
| CGGTTCAACCGGAACAAGAG+CGG | + | chr3.1:64201114-64201133 | MS.gene70607:CDS | 55.0% | |
| GAGTGACGAGAACCGAAGCA+TGG | - | chr3.1:64200976-64200995 | None:intergenic | 55.0% | |
| GAGTGGTCGGAAATCGTTGC+TGG | + | chr3.1:64201066-64201085 | MS.gene70607:CDS | 55.0% | |
| GGAAACCACCGCTCTTGTTC+CGG | - | chr3.1:64201125-64201144 | None:intergenic | 55.0% | |
| TTCAACCGGAACAAGAGCGG+TGG | + | chr3.1:64201117-64201136 | MS.gene70607:CDS | 55.0% | |
| TTCAGCTCGTTACGTTGCGG+CGG | + | chr3.1:64201251-64201270 | MS.gene70607:CDS | 55.0% | |
| ACTCCACCACCACCGAACCA+TGG | + | chr3.1:64200992-64201011 | MS.gene70607:CDS | 60.0% | |
| AGCGGTGGTTTCCGTCATGC+GGG | + | chr3.1:64201132-64201151 | MS.gene70607:CDS | 60.0% | |
| CACACGCTCCCACCATGGTT+CGG | - | chr3.1:64201007-64201026 | None:intergenic | 60.0% | |
| CACCACCACCGAACCATGGT+GGG | + | chr3.1:64200996-64201015 | MS.gene70607:CDS | 60.0% | |
| GAGCGGTGGTTTCCGTCATG+CGG | + | chr3.1:64201131-64201150 | MS.gene70607:CDS | 60.0% | |
| CCACCACCACCGAACCATGG+TGG | + | chr3.1:64200995-64201014 | MS.gene70607:CDS | 65.0% | |
| GCGGTGGTTTCCGTCATGCG+GGG | + | chr3.1:64201133-64201152 | MS.gene70607:CDS | 65.0% | |
| ! | ACGCTCCCACCATGGTTCGG+TGG | - | chr3.1:64201004-64201023 | None:intergenic | 65.0% |
| !! | CCACCATGGTTCGGTGGTGG+TGG | - | chr3.1:64200998-64201017 | None:intergenic | 65.0% |
| !! | CTCCCACCATGGTTCGGTGG+TGG | - | chr3.1:64201001-64201020 | None:intergenic | 65.0% |
| GCTCGCACACGCTCCCACCA+TGG | - | chr3.1:64201012-64201031 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 64200910 | 64201344 | 64200910 | ID=MS.gene70607 |
| chr3.1 | mRNA | 64200910 | 64201344 | 64200910 | ID=MS.gene70607.t1;Parent=MS.gene70607 |
| chr3.1 | exon | 64200910 | 64201344 | 64200910 | ID=MS.gene70607.t1.exon1;Parent=MS.gene70607.t1 |
| chr3.1 | CDS | 64200910 | 64201344 | 64200910 | ID=cds.MS.gene70607.t1;Parent=MS.gene70607.t1 |
| Gene Sequence |
| Protein sequence |
