Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71044.t1 | XP_003598026.1 | 95.4 | 197 | 9 | 0 | 12 | 208 | 1 | 197 | 2.00E-100 | 375.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71044.t1 | Q9FLF0 | 86.8 | 197 | 26 | 0 | 12 | 208 | 1 | 197 | 9.1e-94 | 344.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71044.t1 | B7FMD4 | 95.4 | 197 | 9 | 0 | 12 | 208 | 1 | 197 | 1.4e-100 | 375.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059840 | MS.gene71044 | 0.998215 | 5.27E-259 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTATTTAATTGTAAGTT+TGG | - | 14500:24222-24241 | MS.gene71044:intron | 10.0% |
| !!! | ATATCATTTTTTGTTTTCAT+AGG | - | 14500:24561-24580 | MS.gene71044:CDS | 15.0% |
| !!! | TATCATTTTTTGTTTTCATA+GGG | - | 14500:24562-24581 | MS.gene71044:CDS | 15.0% |
| ! | ATAAAAGGCAGAAATAAACT+AGG | + | 14500:24483-24502 | MS.gene71044:intergenic | 25.0% |
| !! | ATGTCAATAGAACTTACTTT+TGG | - | 14500:24308-24327 | MS.gene71044:intron | 25.0% |
| !! | GTAAAGGTTTAAGCAAATTT+TGG | + | 14500:24439-24458 | MS.gene71044:intergenic | 25.0% |
| !!! | ATTTTTTGTTTTCATAGGGT+TGG | - | 14500:24566-24585 | MS.gene71044:CDS | 25.0% |
| !!! | GTTTATTTCTGCCTTTTATT+TGG | - | 14500:24484-24503 | MS.gene71044:CDS | 25.0% |
| !!! | TGTCAATAGAACTTACTTTT+GGG | - | 14500:24309-24328 | MS.gene71044:intron | 25.0% |
| !!! | TTTTTTGTTTTCATAGGGTT+GGG | - | 14500:24567-24586 | MS.gene71044:CDS | 25.0% |
| AAACACAAAATTGTGCAAAC+AGG | + | 14500:24353-24372 | MS.gene71043:intergenic | 30.0% | |
| AACACAAAATTGTGCAAACA+GGG | + | 14500:24352-24371 | MS.gene71043:intergenic | 30.0% | |
| AGAAAACCCAACCAAATAAA+AGG | + | 14500:24498-24517 | MS.gene71044:intergenic | 30.0% | |
| ! | ATCTGAGAGTTCGAATTTTT+GGG | - | 14500:24266-24285 | MS.gene71044:intron | 30.0% |
| !! | ATTTCTGCCTTTTATTTGGT+TGG | - | 14500:24488-24507 | MS.gene71044:CDS | 30.0% |
| !! | GTTTAAGCAAATTTTGGCAA+TGG | + | 14500:24433-24452 | MS.gene71044:intergenic | 30.0% |
| !! | TTTCTGCCTTTTATTTGGTT+GGG | - | 14500:24489-24508 | MS.gene71044:CDS | 30.0% |
| ATTCCAGTTTACCGAAACTA+TGG | - | 14500:23801-23820 | MS.gene71044:CDS | 35.0% | |
| CTTCTCATCCAATGTCAAAA+GGG | + | 14500:23973-23992 | MS.gene71044:intergenic | 35.0% | |
| GATCAGATAATCAACGGTAA+AGG | + | 14500:24455-24474 | MS.gene71044:intergenic | 35.0% | |
| TCATCGTTGTGAAACATGTT+TGG | - | 14500:24526-24545 | MS.gene71044:CDS | 35.0% | |
| TCTTCTCATCCAATGTCAAA+AGG | + | 14500:23974-23993 | MS.gene71044:intergenic | 35.0% | |
| TTCCAGTTTACCGAAACTAT+GGG | - | 14500:23802-23821 | MS.gene71044:CDS | 35.0% | |
| TTCTAGCATTGTTACGGATA+CGG | + | 14500:23950-23969 | MS.gene71044:intergenic | 35.0% | |
| TTGAGGAGAATGTTTAAGCA+TGG | - | 14500:24026-24045 | MS.gene71044:intron | 35.0% | |
| ! | AAAGGGTTCTAGCATTGTTA+CGG | + | 14500:23956-23975 | MS.gene71044:intergenic | 35.0% |
| ! | CATCTGAGAGTTCGAATTTT+TGG | - | 14500:24265-24284 | MS.gene71044:intron | 35.0% |
| ! | GTCTAGGATCAGATAATCAA+CGG | + | 14500:24461-24480 | MS.gene71044:intergenic | 35.0% |
| ! | TGAAGGTTTTCCCATAGTTT+CGG | + | 14500:23815-23834 | MS.gene71044:intergenic | 35.0% |
| AGTTTCGGTAAACTGGAATG+TGG | + | 14500:23800-23819 | MS.gene71044:intergenic | 40.0% | |
| CAGACTCTTGTCTTCAAATC+TGG | - | 14500:24125-24144 | MS.gene71044:intron | 40.0% | |
| GAACAAGCTCGATTATGTGT+TGG | - | 14500:24067-24086 | MS.gene71044:intron | 40.0% | |
| GGCAGAAATAAACTAGGTCT+AGG | + | 14500:24477-24496 | MS.gene71044:intergenic | 40.0% | |
| GTTTCGGTAAACTGGAATGT+GGG | + | 14500:23799-23818 | MS.gene71044:intergenic | 40.0% | |
| TCTTGTCTTCAAATCTGGCA+TGG | - | 14500:24130-24149 | MS.gene71044:intron | 40.0% | |
| TGAAGACAAGAGTCTGAAGA+CGG | + | 14500:24121-24140 | MS.gene71044:intergenic | 40.0% | |
| TTATCAGGCAGAGACACATT+AGG | - | 14500:24180-24199 | MS.gene71044:intron | 40.0% | |
| TTCCCATAGTTTCGGTAAAC+TGG | + | 14500:23807-23826 | MS.gene71044:intergenic | 40.0% | |
| TTTCGGTAAACTGGAATGTG+GGG | + | 14500:23798-23817 | MS.gene71044:intergenic | 40.0% | |
| ! | TGCTAGAACCCTTTTGACAT+TGG | - | 14500:23962-23981 | MS.gene71044:intron | 40.0% |
| !! | TTTGTTTTCATAGGGTTGGG+AGG | - | 14500:24570-24589 | MS.gene71044:CDS | 40.0% |
| AAGAACCCTAGCATGGTGAA+TGG | + | 14500:24162-24181 | MS.gene71043:intergenic | 45.0% | |
| ACCATGCTAGGGTTCTTATC+AGG | - | 14500:24165-24184 | MS.gene71044:intron | 45.0% | |
| AGTGAACATCCCATCCTTCA+TGG | - | 14500:24598-24617 | MS.gene71044:CDS | 45.0% | |
| ATTGTTACGGATACGGCTCA+GGG | + | 14500:23943-23962 | MS.gene71044:intergenic | 45.0% | |
| CAAGTCCATTCACCATGCTA+GGG | - | 14500:24154-24173 | MS.gene71044:intron | 45.0% | |
| CGTGAAGCGAAAGAACCTTA+AGG | - | 14500:24691-24710 | MS.gene71044:CDS | 45.0% | |
| TGAATCAACCCTCACCATGA+AGG | + | 14500:24615-24634 | MS.gene71044:intergenic | 45.0% | |
| ! | TTTGGATGCTGAGTTGAAGC+TGG | - | 14500:23866-23885 | MS.gene71044:CDS | 45.0% |
| ACATCCCATCCTTCATGGTG+AGG | - | 14500:24603-24622 | MS.gene71044:CDS | 50.0% | |
| ATCACCACCAGAAGCCTTCT+TGG | + | 14500:24723-24742 | MS.gene71044:intergenic | 50.0% | |
| CAACCCTCACCATGAAGGAT+GGG | + | 14500:24610-24629 | MS.gene71044:intergenic | 50.0% | |
| CATCCCATCCTTCATGGTGA+GGG | - | 14500:24604-24623 | MS.gene71044:CDS | 50.0% | |
| CATTGTTACGGATACGGCTC+AGG | + | 14500:23944-23963 | MS.gene71044:intergenic | 50.0% | |
| CCAAGTCCATTCACCATGCT+AGG | - | 14500:24153-24172 | MS.gene71044:intron | 50.0% | |
| CCTTAAGGCTGCAACCAAGA+AGG | - | 14500:24706-24725 | MS.gene71044:CDS | 50.0% | |
| CCTTCTTGGTTGCAGCCTTA+AGG | + | 14500:24709-24728 | MS.gene71044:intergenic | 50.0% | |
| GCCTGATAAGAACCCTAGCA+TGG | + | 14500:24169-24188 | MS.gene71044:intergenic | 50.0% | |
| GTGTAAGAGAGAGCTGTGGA+GGG | - | 14500:23908-23927 | MS.gene71044:CDS | 50.0% | |
| TCAACCCTCACCATGAAGGA+TGG | + | 14500:24611-24630 | MS.gene71044:intergenic | 50.0% | |
| TCCAAACGCTCCTTCTCGTA+TGG | + | 14500:23852-23871 | MS.gene71044:intergenic | 50.0% | |
| TTGAAGCTGGTCGGAGAGTA+TGG | - | 14500:23879-23898 | MS.gene71044:CDS | 50.0% | |
| ! | AAGAAGGCTTCTGGTGGTGA+TGG | - | 14500:24722-24741 | MS.gene71044:CDS | 50.0% |
| ! | CCTAGCATGGTGAATGGACT+TGG | + | 14500:24156-24175 | MS.gene71044:intergenic | 50.0% |
| ! | GATGCTGAGTTGAAGCTGGT+CGG | - | 14500:23870-23889 | MS.gene71044:CDS | 50.0% |
| ! | TGGTCGGAGAGTATGGTCTT+CGG | - | 14500:23886-23905 | MS.gene71044:CDS | 50.0% |
| ! | TTCGGTGTAAGAGAGAGCTG+TGG | - | 14500:23904-23923 | MS.gene71044:CDS | 50.0% |
| ! | TTGAGGGTGACGCACTTTTG+AGG | - | 14500:24009-24028 | MS.gene71044:intron | 50.0% |
| ! | TTTTCATAGGGTTGGGAGGC+AGG | - | 14500:24574-24593 | MS.gene71044:CDS | 50.0% |
| ! | TTTTCCCTCACCAGTCCCTT+TGG | - | 14500:24650-24669 | MS.gene71044:CDS | 50.0% |
| !! | TCCATACGAGAAGGAGCGTT+TGG | - | 14500:23848-23867 | MS.gene71044:CDS | 50.0% |
| !! | TTCTGGTGGTGATGGAGATG+AGG | - | 14500:24730-24749 | MS.gene71044:CDS | 50.0% |
| AAGACCACCACCAAAGGGAC+TGG | + | 14500:24663-24682 | MS.gene71043:intergenic | 55.0% | |
| ACCAGGAAGACCACCACCAA+AGG | + | 14500:24669-24688 | MS.gene71044:intergenic | 55.0% | |
| AGCCCTCGTCGGATCTTTGA+GGG | - | 14500:23993-24012 | MS.gene71044:intron | 55.0% | |
| CACCCTCAAAGATCCGACGA+GGG | + | 14500:23998-24017 | MS.gene71044:intergenic | 55.0% | |
| CCAGGAAGACCACCACCAAA+GGG | + | 14500:24668-24687 | MS.gene71044:intergenic | 55.0% | |
| GCAACCAAGAAGGCTTCTGG+TGG | - | 14500:24716-24735 | MS.gene71044:CDS | 55.0% | |
| GCTGCAACCAAGAAGGCTTC+TGG | - | 14500:24713-24732 | MS.gene71044:CDS | 55.0% | |
| GGTGTAAGAGAGAGCTGTGG+AGG | - | 14500:23907-23926 | MS.gene71044:CDS | 55.0% | |
| TCACCCTCAAAGATCCGACG+AGG | + | 14500:23999-24018 | MS.gene71044:intergenic | 55.0% | |
| TCCTTCTCGTATGGACGACG+TGG | + | 14500:23843-23862 | MS.gene71044:intergenic | 55.0% | |
| TGGACGACGTGGCTTCTTGA+AGG | + | 14500:23832-23851 | MS.gene71044:intergenic | 55.0% | |
| TGGATGAGAAGAGCCCTCGT+CGG | - | 14500:23982-24001 | MS.gene71044:intron | 55.0% | |
| TTCTTTCGCTTCACGCGACC+AGG | + | 14500:24686-24705 | MS.gene71044:intergenic | 55.0% | |
| ! | ACCACCAAAGGGACTGGTGA+GGG | + | 14500:24657-24676 | MS.gene71044:intergenic | 55.0% |
| !! | TGGTGGTGATGGAGATGAGG+AGG | - | 14500:24733-24752 | MS.gene71044:CDS | 55.0% |
| CTCACCAGTCCCTTTGGTGG+TGG | - | 14500:24656-24675 | MS.gene71044:CDS | 60.0% | |
| GAGCCCTCGTCGGATCTTTG+AGG | - | 14500:23992-24011 | MS.gene71044:intron | 60.0% | |
| GCCACGTCGTCCATACGAGA+AGG | - | 14500:23839-23858 | MS.gene71044:CDS | 60.0% | |
| TCCCTCACCAGTCCCTTTGG+TGG | - | 14500:24653-24672 | MS.gene71044:CDS | 60.0% | |
| ! | CACCACCAAAGGGACTGGTG+AGG | + | 14500:24658-24677 | MS.gene71044:intergenic | 60.0% |
| !! | CCCTTTGGTGGTGGTCTTCC+TGG | - | 14500:24665-24684 | MS.gene71044:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 14500 | gene | 23758 | 24766 | 23758 | ID=MS.gene71044 |
| 14500 | mRNA | 23758 | 24766 | 23758 | ID=MS.gene71044.t1;Parent=MS.gene71044 |
| 14500 | exon | 24323 | 24766 | 24323 | ID=MS.gene71044.t1.exon1;Parent=MS.gene71044.t1 |
| 14500 | CDS | 24323 | 24766 | 24323 | ID=cds.MS.gene71044.t1;Parent=MS.gene71044.t1 |
| 14500 | exon | 23758 | 23941 | 23758 | ID=MS.gene71044.t1.exon2;Parent=MS.gene71044.t1 |
| 14500 | CDS | 23758 | 23941 | 23758 | ID=cds.MS.gene71044.t1;Parent=MS.gene71044.t1 |
| Gene Sequence |
| Protein sequence |
