AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71221.t1 MTR_3g435130 69.254 335 69 4 1 302 1 334 4.22e-165 462
MS.gene71221.t1 MTR_8g066220 68.563 334 72 3 1 302 1 333 9.85e-164 459
MS.gene71221.t1 MTR_8g066210 68.563 334 57 4 1 302 1 318 3.20e-155 436
MS.gene71221.t1 MTR_0071s0010 63.720 328 86 3 8 302 6 333 1.45e-148 420
MS.gene71221.t1 MTR_3g046670 63.720 328 86 3 8 302 6 333 1.45e-148 420
MS.gene71221.t1 MTR_8g066280 79.255 188 11 1 143 302 22 209 3.87e-102 298
MS.gene71221.t1 MTR_3g013500 46.226 318 139 2 17 302 20 337 4.59e-98 292
MS.gene71221.t1 MTR_7g007710 44.969 318 138 5 21 302 16 332 4.38e-94 282
MS.gene71221.t1 MTR_7g007680 42.902 317 141 6 21 302 22 333 1.21e-89 272
MS.gene71221.t1 MTR_8g040820 43.082 318 145 5 21 302 27 344 7.01e-89 269
MS.gene71221.t1 MTR_8g035720 41.888 339 150 6 1 302 78 406 1.19e-86 265
MS.gene71221.t1 MTR_8g040490 42.368 321 150 4 17 302 23 343 2.34e-86 264
MS.gene71221.t1 MTR_8g040430 41.411 326 154 5 14 302 20 345 2.54e-83 255
MS.gene71221.t1 MTR_8g040520 43.849 317 139 6 17 297 23 336 3.56e-81 249
MS.gene71221.t1 MTR_8g041790 42.154 325 138 8 14 302 15 325 1.36e-79 244
MS.gene71221.t1 MTR_3g434790 63.542 192 42 1 139 302 43 234 1.08e-76 234
MS.gene71221.t1 MTR_3g434790 84.211 57 9 0 1 57 1 57 7.35e-26 102
MS.gene71221.t1 MTR_8g014380 43.310 284 117 8 17 280 22 281 2.58e-68 214
MS.gene71221.t1 MTR_8g040250 39.677 310 124 8 25 302 15 293 2.42e-67 213
MS.gene71221.t1 MTR_8g040820 42.857 224 96 4 111 302 22 245 1.29e-56 183
MS.gene71221.t1 MTR_8g040430 42.466 219 94 3 116 302 27 245 1.39e-53 175
MS.gene71221.t1 MTR_8g040730 48.731 197 90 6 57 248 24 214 1.53e-47 167
MS.gene71221.t1 MTR_8g040730 44.872 156 77 4 23 172 343 495 2.09e-35 134
MS.gene71221.t1 MTR_8g066190 69.231 91 18 1 222 302 2 92 1.33e-32 116
MS.gene71221.t1 MTR_8g040710 40.268 149 59 2 174 292 134 282 4.70e-28 110
MS.gene71221.t1 MTR_8g040315 37.692 130 54 2 200 302 3 132 1.58e-19 83.2
MS.gene71221.t1 MTR_8g066200 77.778 36 8 0 148 183 25 60 4.20e-14 66.6
MS.gene71221.t1 MTR_8g040410 43.662 71 30 1 214 274 75 145 3.86e-12 63.5
MS.gene71221.t1 MTR_8g040410 50.000 56 26 1 67 120 25 80 4.39e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71221.t1 AT5G07010 43.692 325 148 4 12 302 33 356 1.66e-95 286
MS.gene71221.t1 AT2G03760 46.440 323 142 5 9 302 4 324 3.97e-94 282
MS.gene71221.t1 AT3G45070 46.835 316 134 4 20 302 14 328 1.21e-91 275
MS.gene71221.t1 AT3G45070 46.835 316 134 4 20 302 9 323 1.56e-91 275
MS.gene71221.t1 AT3G45070 46.835 316 134 4 20 302 41 355 2.20e-91 276
MS.gene71221.t1 AT5G07000 43.478 322 145 5 17 302 24 344 5.67e-89 269
MS.gene71221.t1 AT3G45080 46.372 317 135 4 20 302 14 329 1.16e-85 260
MS.gene71221.t1 AT2G03770 42.284 324 147 3 12 303 8 323 9.75e-85 258
MS.gene71221.t1 AT1G13420 45.016 311 132 6 26 302 26 331 1.73e-79 244
MS.gene71221.t1 AT1G28170 42.727 330 151 7 7 302 1 326 1.33e-77 239
MS.gene71221.t1 AT2G03750 41.486 323 155 5 12 302 30 350 2.53e-77 239
MS.gene71221.t1 AT5G43690 41.420 338 155 7 1 302 1 331 4.02e-77 238
MS.gene71221.t1 AT4G26280 42.138 318 132 4 16 299 7 306 3.32e-75 233
MS.gene71221.t1 AT1G18590 41.401 314 139 7 26 300 34 341 5.79e-73 228
MS.gene71221.t1 AT2G14920 40.256 313 150 4 26 302 22 333 4.04e-71 223
MS.gene71221.t1 AT1G74100 38.854 314 147 6 26 300 26 333 3.06e-70 221
MS.gene71221.t1 AT2G27570 40.461 304 124 4 16 302 10 273 4.92e-69 216
MS.gene71221.t1 AT1G13430 38.532 327 150 5 26 302 26 351 7.12e-69 218
MS.gene71221.t1 AT1G74090 39.683 315 143 8 26 300 38 345 1.12e-67 215

Find 58 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTCTCTTGTTCCCTTCT+TGG 0.032882 8.3:-36631830 MS.gene71221:CDS
TCCATCAATACCAAGGATTT+TGG 0.169594 8.3:-36632029 MS.gene71221:CDS
GTTGACTCTTTCACAGAATT+TGG 0.172783 8.3:+36631712 None:intergenic
ACCTTTGGATTCTTCTTCTT+TGG 0.195487 8.3:+36631399 None:intergenic
TCGTATTCTTGCAAGTATTT+TGG 0.197192 8.3:+36632120 None:intergenic
GTATCAATTCCTTGCATTCT+TGG 0.204814 8.3:+36632091 None:intergenic
AGCCAAGTGGTACCTGCTTT+TGG 0.223091 8.3:+36631934 None:intergenic
CACTATCACTGAAAGTAAAT+TGG 0.250645 8.3:-36631293 MS.gene71221:CDS
TGCGAAACAAAAGTGTCTTT+TGG 0.289441 8.3:+36631661 None:intergenic
TGTAGAGGTGCGAGTTCTTT+TGG 0.307639 8.3:-36631565 MS.gene71221:CDS
CCCACATTGCCATTAGAAAA+AGG 0.310786 8.3:-36632069 MS.gene71221:CDS
GGGAGCATGTATTGGGATAT+TGG 0.323106 8.3:-36631534 MS.gene71221:CDS
GCCAAAATCCTTGGTATTGA+TGG 0.341469 8.3:+36632028 None:intergenic
CTCTCAAAAGCCTCTTCTAA+TGG 0.341934 8.3:+36631592 None:intergenic
CAATATCTTTGTCATAATAA+AGG 0.348160 8.3:+36631800 None:intergenic
TACTTGCAAGAATACGATTT+AGG 0.377772 8.3:-36632114 MS.gene71221:CDS
GAATTTGGCAATAATTCATA+TGG 0.378063 8.3:+36631727 None:intergenic
GGGATATTGGAAGGAAAGCT+TGG 0.445940 8.3:-36631521 MS.gene71221:CDS
TGCAATCTCCAACTTGGCCT+AGG 0.445986 8.3:+36631353 None:intergenic
CAAGAAGGGAACAAGAGAAT+GGG 0.448312 8.3:+36631831 None:intergenic
GGGAACAAGAGAATGGGGAT+TGG 0.451880 8.3:+36631837 None:intergenic
ATATGTGTAGAGAAGAGTCT+TGG 0.476489 8.3:+36631751 None:intergenic
CCAAGAAGGGAACAAGAGAA+TGG 0.483836 8.3:+36631830 None:intergenic
GGTGACAATGAGTCGAGATC+GGG 0.492080 8.3:+36631775 None:intergenic
AGAAGAAGAATCCAAAGGTA+TGG 0.503198 8.3:-36631395 MS.gene71221:CDS
TGTGTAGAGAAGAGTCTTGG+AGG 0.507788 8.3:+36631754 None:intergenic
GGATGGTTGTTATGAATGCT+TGG 0.509541 8.3:+36631871 None:intergenic
AGTGGTAACTAAGAGAATAT+CGG 0.530021 8.3:+36631957 None:intergenic
CTCCAAAAGCAGGTACCACT+TGG 0.530830 8.3:-36631936 MS.gene71221:CDS
AGGTGACAATGAGTCGAGAT+CGG 0.533708 8.3:+36631774 None:intergenic
TGGGGATTGGAAGTGAGTAA+AGG 0.540519 8.3:+36631850 None:intergenic
GTACCACTTGGCTCAAAGCA+TGG 0.551523 8.3:-36631924 MS.gene71221:CDS
AAAGCCTCTTCTAATGGAAG+TGG 0.551607 8.3:+36631598 None:intergenic
TTGTGGCAAGCCAAAATCCT+TGG 0.564001 8.3:+36632019 None:intergenic
ATAAAGGTCATACTCCAAGA+AGG 0.564290 8.3:+36631816 None:intergenic
CGATTTAGGCCAAGAATGCA+AGG 0.571635 8.3:-36632100 MS.gene71221:CDS
TTAGTTACCACTCCAAAAGC+AGG 0.574786 8.3:-36631946 MS.gene71221:CDS
TTTGACAAGATAAAACACCT+TGG 0.581098 8.3:+36631989 None:intergenic
GCATGTATTGGGATATTGGA+AGG 0.583242 8.3:-36631530 MS.gene71221:CDS
TAAAGGTCATACTCCAAGAA+GGG 0.588647 8.3:+36631817 None:intergenic
AAGCTTGGAAAGGCCAGAGA+AGG 0.595580 8.3:-36631506 MS.gene71221:CDS
AAGCTAAGAGCACAAAGTAG+AGG 0.595604 8.3:-36631622 MS.gene71221:CDS
GAAAGTAAATTGGCTACACA+TGG 0.597619 8.3:-36631283 MS.gene71221:CDS
AGGACCACTTCCATTAGAAG+AGG 0.602220 8.3:-36631602 MS.gene71221:CDS
GAATGCTTGGATATTTGTTG+CGG 0.608390 8.3:+36631884 None:intergenic
TGTGAAAGAGTCAACTTGCA+AGG 0.618089 8.3:-36631704 MS.gene71221:CDS
CTTGCCACAAGGATTCTCCA+AGG 0.641069 8.3:-36632006 MS.gene71221:CDS
AAGAAGGGAACAAGAGAATG+GGG 0.641846 8.3:+36631832 None:intergenic
AATCTCCAACTTGGCCTAGG+CGG 0.642233 8.3:+36631356 None:intergenic
GATTGGAAGTGAGTAAAGGA+TGG 0.644416 8.3:+36631854 None:intergenic
AGTCCATGCTTTGAGCCAAG+TGG 0.645676 8.3:+36631921 None:intergenic
TCCTACTTCCATCAATACCA+AGG 0.653031 8.3:-36632036 MS.gene71221:CDS
TCCTTGGTATTGATGGAAGT+AGG 0.653788 8.3:+36632035 None:intergenic
TCCAAAGAAGAAGAATCCAA+AGG 0.663515 8.3:-36631400 MS.gene71221:CDS
AACACCTTGGAGAATCCTTG+TGG 0.666075 8.3:+36632002 None:intergenic
ATTGGAAGGAAAGCTTGGAA+AGG 0.672349 8.3:-36631516 MS.gene71221:CDS
AAAGTAAATTGGCTACACAT+GGG 0.678157 8.3:-36631282 MS.gene71221:CDS
GAAAGAGTCAACTTGCAAGG+TGG 0.777827 8.3:-36631701 MS.gene71221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTCTTTCAAATAAAAACT+AGG + chr8.3:36631975-36631994 None:intergenic 20.0%
!! CAATATCTTTGTCATAATAA+AGG + chr8.3:36631623-36631642 None:intergenic 20.0%
! GAATTTGGCAATAATTCATA+TGG + chr8.3:36631696-36631715 None:intergenic 25.0%
AAAGTAAATTGGCTACACAT+GGG - chr8.3:36632138-36632157 MS.gene71221:CDS 30.0%
AGTGGTAACTAAGAGAATAT+CGG + chr8.3:36631466-36631485 None:intergenic 30.0%
CACTATCACTGAAAGTAAAT+TGG - chr8.3:36632127-36632146 MS.gene71221:CDS 30.0%
TACTTGCAAGAATACGATTT+AGG - chr8.3:36631306-36631325 MS.gene71221:CDS 30.0%
TTTGACAAGATAAAACACCT+TGG + chr8.3:36631434-36631453 None:intergenic 30.0%
! AAAGAGATTGCTGAGTTTTT+GGG - chr8.3:36631987-36632006 MS.gene71221:CDS 30.0%
! GTAATCCATCCTTTTTCTAA+TGG + chr8.3:36631363-36631382 None:intergenic 30.0%
! TCGTATTCTTGCAAGTATTT+TGG + chr8.3:36631303-36631322 None:intergenic 30.0%
!! GTATTTGTTTTCCATACCTT+TGG + chr8.3:36632039-36632058 None:intergenic 30.0%
ACCTTTGGATTCTTCTTCTT+TGG + chr8.3:36632024-36632043 None:intergenic 35.0%
AGAAGAAGAATCCAAAGGTA+TGG - chr8.3:36632025-36632044 MS.gene71221:CDS 35.0%
ATAAAGGTCATACTCCAAGA+AGG + chr8.3:36631607-36631626 None:intergenic 35.0%
ATATGTGTAGAGAAGAGTCT+TGG + chr8.3:36631672-36631691 None:intergenic 35.0%
CATTGCCATTAGAAAAAGGA+TGG - chr8.3:36631355-36631374 MS.gene71221:CDS 35.0%
GAAAGTAAATTGGCTACACA+TGG - chr8.3:36632137-36632156 MS.gene71221:CDS 35.0%
GTATCAATTCCTTGCATTCT+TGG + chr8.3:36631332-36631351 None:intergenic 35.0%
TAAAGGTCATACTCCAAGAA+GGG + chr8.3:36631606-36631625 None:intergenic 35.0%
TCCAAAGAAGAAGAATCCAA+AGG - chr8.3:36632020-36632039 MS.gene71221:CDS 35.0%
TGCAAAAATGTTCTCACAAC+TGG - chr8.3:36632086-36632105 MS.gene71221:CDS 35.0%
TTCAAAAACATCACCTTCTC+TGG + chr8.3:36631930-36631949 None:intergenic 35.0%
! CATTTTTGCAATCTCCAACT+TGG + chr8.3:36632076-36632095 None:intergenic 35.0%
! CCTTTTTCTAATGGCAATGT+GGG + chr8.3:36631354-36631373 None:intergenic 35.0%
! GAAAGAGATTGCTGAGTTTT+TGG - chr8.3:36631986-36632005 MS.gene71221:CDS 35.0%
! GAATGCTTGGATATTTGTTG+CGG + chr8.3:36631539-36631558 None:intergenic 35.0%
! GAGTTCTTTTGGACTGTTTT+GGG - chr8.3:36631866-36631885 MS.gene71221:CDS 35.0%
! GTTGACTCTTTCACAGAATT+TGG + chr8.3:36631711-36631730 None:intergenic 35.0%
! TCCATCAATACCAAGGATTT+TGG - chr8.3:36631391-36631410 MS.gene71221:CDS 35.0%
! TCCTTTTTCTAATGGCAATG+TGG + chr8.3:36631355-36631374 None:intergenic 35.0%
!! AAGACACTTTTGTTTCGCAT+TGG - chr8.3:36631763-36631782 MS.gene71221:CDS 35.0%
!! GCTTTTGAGAGTTTTTGTAG+AGG - chr8.3:36631840-36631859 MS.gene71221:CDS 35.0%
!!! AACAAATACTTTTTCCGCCT+AGG - chr8.3:36632050-36632069 MS.gene71221:CDS 35.0%
!!! TGCGAAACAAAAGTGTCTTT+TGG + chr8.3:36631762-36631781 None:intergenic 35.0%
AAAGCCTCTTCTAATGGAAG+TGG + chr8.3:36631825-36631844 None:intergenic 40.0%
AAGAAGGGAACAAGAGAATG+GGG + chr8.3:36631591-36631610 None:intergenic 40.0%
ATTGGAAGGAAAGCTTGGAA+AGG - chr8.3:36631904-36631923 MS.gene71221:CDS 40.0%
CAAGAAGGGAACAAGAGAAT+GGG + chr8.3:36631592-36631611 None:intergenic 40.0%
CCCACATTGCCATTAGAAAA+AGG - chr8.3:36631351-36631370 MS.gene71221:CDS 40.0%
CTCTCAAAAGCCTCTTCTAA+TGG + chr8.3:36631831-36631850 None:intergenic 40.0%
GATTGGAAGTGAGTAAAGGA+TGG + chr8.3:36631569-36631588 None:intergenic 40.0%
GCATGTATTGGGATATTGGA+AGG - chr8.3:36631890-36631909 MS.gene71221:CDS 40.0%
GGATGGTTGTTATGAATGCT+TGG + chr8.3:36631552-36631571 None:intergenic 40.0%
TCCTACTTCCATCAATACCA+AGG - chr8.3:36631384-36631403 MS.gene71221:CDS 40.0%
TGTGAAAGAGTCAACTTGCA+AGG - chr8.3:36631716-36631735 MS.gene71221:CDS 40.0%
TTAGTTACCACTCCAAAAGC+AGG - chr8.3:36631474-36631493 MS.gene71221:CDS 40.0%
! AAGCTAAGAGCACAAAGTAG+AGG - chr8.3:36631798-36631817 MS.gene71221:CDS 40.0%
! CGAGTTCTTTTGGACTGTTT+TGG - chr8.3:36631865-36631884 MS.gene71221:CDS 40.0%
! GCCAAAATCCTTGGTATTGA+TGG + chr8.3:36631395-36631414 None:intergenic 40.0%
!! TCCTTGGTATTGATGGAAGT+AGG + chr8.3:36631388-36631407 None:intergenic 40.0%
!!! ACTGTTTTGGGAGCATGTAT+TGG - chr8.3:36631878-36631897 MS.gene71221:CDS 40.0%
!!! ATTGCTGAGTTTTTGGGTTG+TGG - chr8.3:36631993-36632012 MS.gene71221:CDS 40.0%
!!! CTGTTTTGGGAGCATGTATT+GGG - chr8.3:36631879-36631898 MS.gene71221:CDS 40.0%
AACACCTTGGAGAATCCTTG+TGG + chr8.3:36631421-36631440 None:intergenic 45.0%
AGGACCACTTCCATTAGAAG+AGG - chr8.3:36631818-36631837 MS.gene71221:CDS 45.0%
AGGTGACAATGAGTCGAGAT+CGG + chr8.3:36631649-36631668 None:intergenic 45.0%
CCAAGAAGGGAACAAGAGAA+TGG + chr8.3:36631593-36631612 None:intergenic 45.0%
CCATTCTCTTGTTCCCTTCT+TGG - chr8.3:36631590-36631609 MS.gene71221:CDS 45.0%
CGATTTAGGCCAAGAATGCA+AGG - chr8.3:36631320-36631339 MS.gene71221:CDS 45.0%
GAAAGAGTCAACTTGCAAGG+TGG - chr8.3:36631719-36631738 MS.gene71221:CDS 45.0%
GGGATATTGGAAGGAAAGCT+TGG - chr8.3:36631899-36631918 MS.gene71221:CDS 45.0%
TGGGGATTGGAAGTGAGTAA+AGG + chr8.3:36631573-36631592 None:intergenic 45.0%
TGTGTAGAGAAGAGTCTTGG+AGG + chr8.3:36631669-36631688 None:intergenic 45.0%
TTGTGGCAAGCCAAAATCCT+TGG + chr8.3:36631404-36631423 None:intergenic 45.0%
! GGGAGCATGTATTGGGATAT+TGG - chr8.3:36631886-36631905 MS.gene71221:CDS 45.0%
!! TGTAGAGGTGCGAGTTCTTT+TGG - chr8.3:36631855-36631874 MS.gene71221:CDS 45.0%
!!! AAGGATTTTGGCTTGCCACA+AGG - chr8.3:36631403-36631422 MS.gene71221:CDS 45.0%
AAGCTTGGAAAGGCCAGAGA+AGG - chr8.3:36631914-36631933 MS.gene71221:CDS 50.0%
AATCTCCAACTTGGCCTAGG+CGG + chr8.3:36632067-36632086 None:intergenic 50.0%
CTCCAAAAGCAGGTACCACT+TGG - chr8.3:36631484-36631503 MS.gene71221:CDS 50.0%
CTTGCCACAAGGATTCTCCA+AGG - chr8.3:36631414-36631433 MS.gene71221:CDS 50.0%
GGGAACAAGAGAATGGGGAT+TGG + chr8.3:36631586-36631605 None:intergenic 50.0%
GGTGACAATGAGTCGAGATC+GGG + chr8.3:36631648-36631667 None:intergenic 50.0%
GTACCACTTGGCTCAAAGCA+TGG - chr8.3:36631496-36631515 MS.gene71221:CDS 50.0%
TGCAATCTCCAACTTGGCCT+AGG + chr8.3:36632070-36632089 None:intergenic 50.0%
! AGCCAAGTGGTACCTGCTTT+TGG + chr8.3:36631489-36631508 None:intergenic 50.0%
! AGTCCATGCTTTGAGCCAAG+TGG + chr8.3:36631502-36631521 None:intergenic 50.0%
! TTTTTCCGCCTAGGCCAAGT+TGG - chr8.3:36632059-36632078 MS.gene71221:CDS 50.0%
!! AGTGGTACCTGCTTTTGGAG+TGG + chr8.3:36631484-36631503 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 36631264 36632178 36631264 ID=MS.gene71221
chr8.3 mRNA 36631264 36632178 36631264 ID=MS.gene71221.t1;Parent=MS.gene71221
chr8.3 exon 36631264 36632178 36631264 ID=MS.gene71221.t1.exon1;Parent=MS.gene71221.t1
chr8.3 CDS 36631264 36632178 36631264 ID=cds.MS.gene71221.t1;Parent=MS.gene71221.t1
Gene Sequence

>MS.gene71221

ATGGCAAAATCAAGTGATGTTGAAAACTCTCTTGTGCCAAAATACTTGCAAGAATACGATTTAGGCCAAGAATGCAAGGAATTGATACCCACATTGCCATTAGAAAAAGGATGGATTACATCCTACTTCCATCAATACCAAGGATTTTGGCTTGCCACAAGGATTCTCCAAGGTGTTTTATCTTGTCAAAAGAACTTTACCGATATTCTCTTAGTTACCACTCCAAAAGCAGGTACCACTTGGCTCAAAGCATGGACTTTTGCTTTGCTAAACCGCAACAAATATCCAAGCATTCATAACAACCATCCTTTACTCACTTCCAATCCCCATTCTCTTGTTCCCTTCTTGGAGTATGACCTTTATTATGACAAAGATATTGTTCCCGATCTCGACTCATTGTCACCTCCAAGACTCTTCTCTACACATATTCCATATGAATTATTGCCAAATTCTGTGAAAGAGTCAACTTGCAAGGTGGTGTATCTTTGTAGAGATCCAAAAGACACTTTTGTTTCGCATTGGCATTTCACAAATAAGCTAAGAGCACAAAGTAGAGGACCACTTCCATTAGAAGAGGCTTTTGAGAGTTTTTGTAGAGGTGCGAGTTCTTTTGGACTGTTTTGGGAGCATGTATTGGGATATTGGAAGGAAAGCTTGGAAAGGCCAGAGAAGGTGATGTTTTTGAAATATGAAGAAATGAAAATGAAACCTAGTTTTTATTTGAAAGAGATTGCTGAGTTTTTGGGTTGTGGATTCTCCAAAGAAGAAGAATCCAAAGGTATGGAAAACAAATACTTTTTCCGCCTAGGCCAAGTTGGAGATTGCAAAAATGTTCTCACAACTGGAATGATTAAGCAGTTAAACACTATCACTGAAAGTAAATTGGCTACACATGGGTTGAGATTTTTAGATTGA
Protein sequence

>MS.gene71221.t1

MAKSSDVENSLVPKYLQEYDLGQECKELIPTLPLEKGWITSYFHQYQGFWLATRILQGVLSCQKNFTDILLVTTPKAGTTWLKAWTFALLNRNKYPSIHNNHPLLTSNPHSLVPFLEYDLYYDKDIVPDLDSLSPPRLFSTHIPYELLPNSVKESTCKVVYLCRDPKDTFVSHWHFTNKLRAQSRGPLPLEEAFESFCRGASSFGLFWEHVLGYWKESLERPEKVMFLKYEEMKMKPSFYLKEIAEFLGCGFSKEEESKGMENKYFFRLGQVGDCKNVLTTGMIKQLNTITESKLATHGLRFLD