Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71626.t1 | XP_003592478.1 | 79.4 | 194 | 25 | 1 | 1 | 179 | 1 | 194 | 9.90E-80 | 306.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71626.t1 | Q9SN23 | 68.6 | 118 | 35 | 1 | 1 | 118 | 1 | 116 | 4.8e-43 | 175.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71626.t1 | G7IE59 | 79.4 | 194 | 25 | 1 | 1 | 179 | 1 | 194 | 7.1e-80 | 306.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene71626.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060276 | MS.gene71626 | 0.876265 | 1.60E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71626.t1 | MTR_1g106420 | 89.175 | 194 | 6 | 1 | 1 | 179 | 1 | 194 | 2.12e-119 | 335 |
| MS.gene71626.t1 | MTR_5g015880 | 65.873 | 126 | 40 | 2 | 1 | 126 | 1 | 123 | 2.49e-55 | 174 |
| MS.gene71626.t1 | MTR_4g095600 | 70.085 | 117 | 33 | 1 | 1 | 117 | 1 | 115 | 6.59e-55 | 174 |
| MS.gene71626.t1 | MTR_7g007010 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 7.92e-47 | 155 |
| MS.gene71626.t1 | MTR_6g018270 | 60.577 | 104 | 41 | 0 | 1 | 104 | 3 | 106 | 2.88e-45 | 150 |
| MS.gene71626.t1 | MTR_6g453130 | 40.625 | 160 | 78 | 4 | 1 | 152 | 3 | 153 | 3.67e-38 | 130 |
| MS.gene71626.t1 | MTR_1g110840 | 44.275 | 131 | 58 | 3 | 1 | 125 | 3 | 124 | 3.95e-36 | 125 |
| MS.gene71626.t1 | MTR_6g054150 | 49.057 | 106 | 52 | 1 | 1 | 104 | 3 | 108 | 3.54e-35 | 123 |
| MS.gene71626.t1 | MTR_6g054080 | 41.732 | 127 | 67 | 2 | 1 | 125 | 3 | 124 | 1.26e-33 | 119 |
| MS.gene71626.t1 | MTR_6g005080 | 34.021 | 97 | 59 | 1 | 3 | 99 | 11 | 102 | 6.63e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71626.t1 | AT3G49940 | 68.644 | 118 | 35 | 1 | 1 | 118 | 1 | 116 | 7.27e-55 | 174 |
| MS.gene71626.t1 | AT5G67420 | 67.241 | 116 | 36 | 1 | 1 | 116 | 1 | 114 | 7.12e-54 | 171 |
| MS.gene71626.t1 | AT5G67420 | 67.241 | 116 | 36 | 1 | 1 | 116 | 1 | 114 | 7.12e-54 | 171 |
| MS.gene71626.t1 | AT4G37540 | 55.422 | 166 | 58 | 5 | 1 | 160 | 1 | 156 | 2.31e-52 | 167 |
| MS.gene71626.t1 | AT3G02550 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 5.05e-47 | 154 |
| MS.gene71626.t1 | AT1G67100 | 60.000 | 105 | 42 | 0 | 1 | 105 | 3 | 107 | 2.27e-44 | 147 |
| MS.gene71626.t1 | AT1G68510 | 43.125 | 160 | 87 | 2 | 1 | 160 | 3 | 158 | 1.61e-42 | 142 |
Find 47 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCCCAACTGCTCCATTTAC+TGG | 0.215944 | 1.4:-82355421 | None:intergenic |
| TTTATGACATACATGATCTT+TGG | 0.296751 | 1.4:-82355182 | None:intergenic |
| AGAACTATAAATCCAGTAAA+TGG | 0.325401 | 1.4:+82355409 | MS.gene71626:CDS |
| ATGGAGCAGTTGGGCTGTTA+TGG | 0.333353 | 1.4:+82355428 | MS.gene71626:CDS |
| GTCTTTGTCACCAAGTTCTT+CGG | 0.371969 | 1.4:+82355117 | MS.gene71626:CDS |
| GATCTTTGGTTGGGAGGAAC+AGG | 0.395798 | 1.4:-82355168 | None:intergenic |
| GACATACATGATCTTTGGTT+GGG | 0.427328 | 1.4:-82355177 | None:intergenic |
| CACTTATGTATGAAGCTGTT+GGG | 0.452221 | 1.4:+82355386 | MS.gene71626:CDS |
| TGGCGTTAGCCTGTGCTTGT+GGG | 0.452316 | 1.4:-82355092 | None:intergenic |
| GACACATGAGTCTGAGGAAT+CGG | 0.458754 | 1.4:+82355606 | MS.gene71626:CDS |
| TCCACACCTAACAACTGATC+AGG | 0.464754 | 1.4:-82355526 | None:intergenic |
| GGTGGCACGGCCGAAGAACT+TGG | 0.467396 | 1.4:-82355127 | None:intergenic |
| AATCCAGTAAATGGAGCAGT+TGG | 0.467406 | 1.4:+82355418 | MS.gene71626:CDS |
| GAATCGGAGTCGTCCACGTT+GGG | 0.467550 | 1.4:+82355622 | MS.gene71626:CDS |
| GCACTCCCTGATCAGTTGTT+AGG | 0.477941 | 1.4:+82355520 | MS.gene71626:CDS |
| GTGGCGTTAGCCTGTGCTTG+TGG | 0.478831 | 1.4:-82355093 | None:intergenic |
| TGACATACATGATCTTTGGT+TGG | 0.489850 | 1.4:-82355178 | None:intergenic |
| CCCTGATCAGTTGTTAGGTG+TGG | 0.497837 | 1.4:+82355525 | MS.gene71626:CDS |
| ATCCAGTAAATGGAGCAGTT+GGG | 0.536591 | 1.4:+82355419 | MS.gene71626:CDS |
| ATAAGAATAGTGTAAGGAGT+TGG | 0.538169 | 1.4:-82355705 | None:intergenic |
| TCACTTATGTATGAAGCTGT+TGG | 0.539852 | 1.4:+82355385 | MS.gene71626:CDS |
| TGCATCCCTTTCTCAAAACT+CGG | 0.542491 | 1.4:-82355026 | None:intergenic |
| GAACAAGTGCTGAGAGGTAA+CGG | 0.548928 | 1.4:+82355484 | MS.gene71626:CDS |
| ATATTCATAAGAATAGTGTA+AGG | 0.549039 | 1.4:-82355711 | None:intergenic |
| ATGCTAATTCTCAAACTCAA+AGG | 0.552063 | 1.4:+82355680 | MS.gene71626:CDS |
| GATACAAAACCCACAAGCAC+AGG | 0.556238 | 1.4:+82355083 | MS.gene71626:CDS |
| TATAATAAAATGAGTTGCAA+CGG | 0.559441 | 1.4:+82355000 | None:intergenic |
| TTTGAGAAAGGGATGCAGTG+AGG | 0.560200 | 1.4:+82355032 | MS.gene71626:CDS |
| CAGCTTCATACATAAGTGAC+TGG | 0.569517 | 1.4:-82355381 | None:intergenic |
| ATACATGATCTTTGGTTGGG+AGG | 0.585857 | 1.4:-82355174 | None:intergenic |
| GGCTGTTATGGACTGGAAAG+TGG | 0.603769 | 1.4:+82355440 | MS.gene71626:CDS |
| GAAAAGACATGAGGGTGGCA+CGG | 0.613200 | 1.4:-82355140 | None:intergenic |
| GCAGTTGGGCTGTTATGGAC+TGG | 0.615948 | 1.4:+82355433 | MS.gene71626:CDS |
| CCACACCTAACAACTGATCA+GGG | 0.634962 | 1.4:-82355525 | None:intergenic |
| TGCGGAGACACATGAGTCTG+AGG | 0.638167 | 1.4:+82355600 | MS.gene71626:CDS |
| GGAATCGGAGTCGTCCACGT+TGG | 0.646426 | 1.4:+82355621 | MS.gene71626:CDS |
| AAGTGGCAATTCTGTCAACT+TGG | 0.652925 | 1.4:+82355457 | MS.gene71626:CDS |
| TTCTGTTCTACTTCCCAACG+TGG | 0.659625 | 1.4:-82355635 | None:intergenic |
| CAGGTGAAAGAAAAGACATG+AGG | 0.660626 | 1.4:-82355149 | None:intergenic |
| AGGTGAAAGAAAAGACATGA+GGG | 0.666800 | 1.4:-82355148 | None:intergenic |
| TAACAACTGATCAGGGAGTG+CGG | 0.677605 | 1.4:-82355518 | None:intergenic |
| CCAAGCAGAAAAGCAACGTG+CGG | 0.683012 | 1.4:+82355582 | MS.gene71626:CDS |
| TGCTACGAGATTGTCTAACG+TGG | 0.685370 | 1.4:+82355061 | MS.gene71626:CDS |
| GAACTTGGTGACAAAGACAG+TGG | 0.712149 | 1.4:-82355112 | None:intergenic |
| TGAAAGAAAAGACATGAGGG+TGG | 0.725920 | 1.4:-82355145 | None:intergenic |
| GGTGTTGAACAAGTGCTGAG+AGG | 0.756300 | 1.4:+82355478 | MS.gene71626:CDS |
| CAAGTGCTGAGAGGTAACGG+CGG | 0.757236 | 1.4:+82355487 | MS.gene71626:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTAATTTGTATGTATATAT+AGG | + | chr1.4:82355277-82355296 | MS.gene71626:intron | 15.0% |
| !! | ATTCAATTACTCTATATTAC+AGG | + | chr1.4:82355352-82355371 | MS.gene71626:intron | 20.0% |
| !!! | ACAAATTAGTTTGATGTATT+TGG | - | chr1.4:82355267-82355286 | None:intergenic | 20.0% |
| ! | AGAACTATAAATCCAGTAAA+TGG | + | chr1.4:82355409-82355428 | MS.gene71626:CDS | 25.0% |
| ! | TTTATGACATACATGATCTT+TGG | - | chr1.4:82355185-82355204 | None:intergenic | 25.0% |
| ATAAGAATAGTGTAAGGAGT+TGG | - | chr1.4:82355708-82355727 | None:intergenic | 30.0% | |
| ATGCTAATTCTCAAACTCAA+AGG | + | chr1.4:82355680-82355699 | MS.gene71626:CDS | 30.0% | |
| AGGTGAAAGAAAAGACATGA+GGG | - | chr1.4:82355151-82355170 | None:intergenic | 35.0% | |
| CACTTATGTATGAAGCTGTT+GGG | + | chr1.4:82355386-82355405 | MS.gene71626:CDS | 35.0% | |
| GACATACATGATCTTTGGTT+GGG | - | chr1.4:82355180-82355199 | None:intergenic | 35.0% | |
| TCACTTATGTATGAAGCTGT+TGG | + | chr1.4:82355385-82355404 | MS.gene71626:CDS | 35.0% | |
| TGACATACATGATCTTTGGT+TGG | - | chr1.4:82355181-82355200 | None:intergenic | 35.0% | |
| AAGTGGCAATTCTGTCAACT+TGG | + | chr1.4:82355457-82355476 | MS.gene71626:CDS | 40.0% | |
| AATCCAGTAAATGGAGCAGT+TGG | + | chr1.4:82355418-82355437 | MS.gene71626:CDS | 40.0% | |
| ATACATGATCTTTGGTTGGG+AGG | - | chr1.4:82355177-82355196 | None:intergenic | 40.0% | |
| ATCCAGTAAATGGAGCAGTT+GGG | + | chr1.4:82355419-82355438 | MS.gene71626:CDS | 40.0% | |
| CAGCTTCATACATAAGTGAC+TGG | - | chr1.4:82355384-82355403 | None:intergenic | 40.0% | |
| CAGGTGAAAGAAAAGACATG+AGG | - | chr1.4:82355152-82355171 | None:intergenic | 40.0% | |
| GTCTTTGTCACCAAGTTCTT+CGG | + | chr1.4:82355117-82355136 | MS.gene71626:CDS | 40.0% | |
| TGAAAGAAAAGACATGAGGG+TGG | - | chr1.4:82355148-82355167 | None:intergenic | 40.0% | |
| TGCATCCCTTTCTCAAAACT+CGG | - | chr1.4:82355029-82355048 | None:intergenic | 40.0% | |
| CCACACCTAACAACTGATCA+GGG | - | chr1.4:82355528-82355547 | None:intergenic | 45.0% | |
| GACACATGAGTCTGAGGAAT+CGG | + | chr1.4:82355606-82355625 | MS.gene71626:CDS | 45.0% | |
| GATACAAAACCCACAAGCAC+AGG | + | chr1.4:82355083-82355102 | MS.gene71626:CDS | 45.0% | |
| TAACAACTGATCAGGGAGTG+CGG | - | chr1.4:82355521-82355540 | None:intergenic | 45.0% | |
| TCCACACCTAACAACTGATC+AGG | - | chr1.4:82355529-82355548 | None:intergenic | 45.0% | |
| TGCTACGAGATTGTCTAACG+TGG | + | chr1.4:82355061-82355080 | MS.gene71626:CDS | 45.0% | |
| TTCTGTTCTACTTCCCAACG+TGG | - | chr1.4:82355638-82355657 | None:intergenic | 45.0% | |
| TTTGAGAAAGGGATGCAGTG+AGG | + | chr1.4:82355032-82355051 | MS.gene71626:CDS | 45.0% | |
| ! | GAACTTGGTGACAAAGACAG+TGG | - | chr1.4:82355115-82355134 | None:intergenic | 45.0% |
| !! | GAACAAGTGCTGAGAGGTAA+CGG | + | chr1.4:82355484-82355503 | MS.gene71626:CDS | 45.0% |
| !!! | GGTTGCCGAGTTTTGAGAAA+GGG | + | chr1.4:82355021-82355040 | MS.gene71626:CDS | 45.0% |
| AGCCCAACTGCTCCATTTAC+TGG | - | chr1.4:82355424-82355443 | None:intergenic | 50.0% | |
| ATGGAGCAGTTGGGCTGTTA+TGG | + | chr1.4:82355428-82355447 | MS.gene71626:CDS | 50.0% | |
| CCAAGCAGAAAAGCAACGTG+CGG | + | chr1.4:82355582-82355601 | MS.gene71626:CDS | 50.0% | |
| CCCTGATCAGTTGTTAGGTG+TGG | + | chr1.4:82355525-82355544 | MS.gene71626:CDS | 50.0% | |
| GAAAAGACATGAGGGTGGCA+CGG | - | chr1.4:82355143-82355162 | None:intergenic | 50.0% | |
| GATCTTTGGTTGGGAGGAAC+AGG | - | chr1.4:82355171-82355190 | None:intergenic | 50.0% | |
| GCACTCCCTGATCAGTTGTT+AGG | + | chr1.4:82355520-82355539 | MS.gene71626:CDS | 50.0% | |
| !! | GGCTGTTATGGACTGGAAAG+TGG | + | chr1.4:82355440-82355459 | MS.gene71626:CDS | 50.0% |
| !! | GGTGTTGAACAAGTGCTGAG+AGG | + | chr1.4:82355478-82355497 | MS.gene71626:CDS | 50.0% |
| !!! | CGGTTGCCGAGTTTTGAGAA+AGG | + | chr1.4:82355020-82355039 | MS.gene71626:CDS | 50.0% |
| GAATCGGAGTCGTCCACGTT+GGG | + | chr1.4:82355622-82355641 | MS.gene71626:CDS | 55.0% | |
| GCAGTTGGGCTGTTATGGAC+TGG | + | chr1.4:82355433-82355452 | MS.gene71626:CDS | 55.0% | |
| TGCGGAGACACATGAGTCTG+AGG | + | chr1.4:82355600-82355619 | MS.gene71626:CDS | 55.0% | |
| ! | TGGCGTTAGCCTGTGCTTGT+GGG | - | chr1.4:82355095-82355114 | None:intergenic | 55.0% |
| !! | CAAGTGCTGAGAGGTAACGG+CGG | + | chr1.4:82355487-82355506 | MS.gene71626:CDS | 55.0% |
| !!! | CCGCACGTTGCTTTTCTGCT+TGG | - | chr1.4:82355585-82355604 | None:intergenic | 55.0% |
| GGAATCGGAGTCGTCCACGT+TGG | + | chr1.4:82355621-82355640 | MS.gene71626:CDS | 60.0% | |
| ! | GTGGCGTTAGCCTGTGCTTG+TGG | - | chr1.4:82355096-82355115 | None:intergenic | 60.0% |
| GGTGGCACGGCCGAAGAACT+TGG | - | chr1.4:82355130-82355149 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 82355009 | 82355729 | 82355009 | ID=MS.gene71626 |
| chr1.4 | mRNA | 82355009 | 82355729 | 82355009 | ID=MS.gene71626.t1;Parent=MS.gene71626 |
| chr1.4 | exon | 82355009 | 82355192 | 82355009 | ID=MS.gene71626.t1.exon1;Parent=MS.gene71626.t1 |
| chr1.4 | CDS | 82355009 | 82355192 | 82355009 | ID=cds.MS.gene71626.t1;Parent=MS.gene71626.t1 |
| chr1.4 | exon | 82355374 | 82355729 | 82355374 | ID=MS.gene71626.t1.exon2;Parent=MS.gene71626.t1 |
| chr1.4 | CDS | 82355374 | 82355729 | 82355374 | ID=cds.MS.gene71626.t1;Parent=MS.gene71626.t1 |
| Gene Sequence |
| Protein sequence |
