Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060276.t1 | XP_003592478.1 | 86.6 | 172 | 6 | 3 | 1 | 172 | 1 | 155 | 6.40E-76 | 293.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060276.t1 | Q9SN23 | 68.6 | 118 | 35 | 1 | 1 | 118 | 1 | 116 | 4.7e-43 | 175.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060276.t1 | G7IE59 | 86.6 | 172 | 6 | 3 | 1 | 172 | 1 | 155 | 4.6e-76 | 293.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene060276.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060276 | MS.gene71626 | 0.876265 | 1.60E-68 | -1.69E-46 |
| MS.gene060276 | MS.gene91299 | 0.880869 | 3.85E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060276.t1 | MTR_1g106420 | 86.628 | 172 | 6 | 3 | 1 | 172 | 1 | 155 | 1.31e-103 | 295 |
| MS.gene060276.t1 | MTR_5g015880 | 62.406 | 133 | 47 | 2 | 1 | 133 | 1 | 130 | 9.18e-55 | 173 |
| MS.gene060276.t1 | MTR_4g095600 | 53.448 | 174 | 76 | 3 | 1 | 174 | 1 | 169 | 2.00e-54 | 172 |
| MS.gene060276.t1 | MTR_7g007010 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 4.50e-46 | 153 |
| MS.gene060276.t1 | MTR_6g018270 | 43.902 | 164 | 87 | 2 | 1 | 164 | 3 | 161 | 4.96e-45 | 149 |
| MS.gene060276.t1 | MTR_6g453130 | 46.565 | 131 | 55 | 3 | 1 | 125 | 3 | 124 | 9.59e-38 | 129 |
| MS.gene060276.t1 | MTR_1g110840 | 39.157 | 166 | 84 | 5 | 1 | 157 | 3 | 160 | 3.32e-37 | 128 |
| MS.gene060276.t1 | MTR_6g054150 | 43.307 | 127 | 65 | 2 | 1 | 125 | 3 | 124 | 5.41e-35 | 122 |
| MS.gene060276.t1 | MTR_6g054080 | 41.732 | 127 | 67 | 2 | 1 | 125 | 3 | 124 | 1.24e-33 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060276.t1 | AT3G49940 | 68.644 | 118 | 35 | 1 | 1 | 118 | 1 | 116 | 5.14e-54 | 172 |
| MS.gene060276.t1 | AT5G67420 | 67.241 | 116 | 36 | 1 | 1 | 116 | 1 | 114 | 4.05e-53 | 169 |
| MS.gene060276.t1 | AT5G67420 | 67.241 | 116 | 36 | 1 | 1 | 116 | 1 | 114 | 4.05e-53 | 169 |
| MS.gene060276.t1 | AT4G37540 | 60.902 | 133 | 47 | 2 | 1 | 129 | 1 | 132 | 1.80e-50 | 162 |
| MS.gene060276.t1 | AT3G02550 | 61.538 | 104 | 40 | 0 | 1 | 104 | 3 | 106 | 3.12e-46 | 152 |
| MS.gene060276.t1 | AT1G67100 | 60.000 | 105 | 42 | 0 | 1 | 105 | 3 | 107 | 5.67e-44 | 145 |
| MS.gene060276.t1 | AT1G68510 | 41.279 | 172 | 91 | 3 | 1 | 164 | 3 | 172 | 1.01e-41 | 140 |
Find 46 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACAGCAAGAGTTTATTT+TGG | 0.186602 | 1.2:+79405037 | MS.gene060276:CDS |
| AGCCCAACTGCTCCATTTAC+TGG | 0.215944 | 1.2:-79404876 | None:intergenic |
| TTTATGACATACATGATCTT+TGG | 0.296751 | 1.2:-79404636 | None:intergenic |
| AGAACTATAAATCCAGTAAA+TGG | 0.325401 | 1.2:+79404864 | MS.gene060276:CDS |
| ATGGAGCAGTTGGGCTGTTA+TGG | 0.333353 | 1.2:+79404883 | MS.gene060276:CDS |
| GTCTTTGTCACCAAGTTCTT+CGG | 0.371969 | 1.2:+79404571 | MS.gene060276:CDS |
| TGTAATGAAGAAGCACAATC+TGG | 0.379276 | 1.2:+79405068 | MS.gene060276:CDS |
| GATCTTTGGTTGGGAGGAAC+AGG | 0.395798 | 1.2:-79404622 | None:intergenic |
| GACATACATGATCTTTGGTT+GGG | 0.427328 | 1.2:-79404631 | None:intergenic |
| CACTTATGTATGAAGCTGTT+GGG | 0.452221 | 1.2:+79404841 | MS.gene060276:CDS |
| TGGCGTTAGCCTGTGCTTGT+GGG | 0.452316 | 1.2:-79404546 | None:intergenic |
| TCCACACCTAACAACTGATC+AGG | 0.464754 | 1.2:-79404981 | None:intergenic |
| GGTGGCACGGCCGAAGAACT+TGG | 0.467396 | 1.2:-79404581 | None:intergenic |
| AATCCAGTAAATGGAGCAGT+TGG | 0.467406 | 1.2:+79404873 | MS.gene060276:CDS |
| GCACTCCCTGATCAGTTGTT+AGG | 0.477941 | 1.2:+79404975 | MS.gene060276:CDS |
| GTGGCGTTAGCCTGTGCTTG+TGG | 0.478831 | 1.2:-79404547 | None:intergenic |
| TGACATACATGATCTTTGGT+TGG | 0.489850 | 1.2:-79404632 | None:intergenic |
| CCCTGATCAGTTGTTAGGTG+TGG | 0.497837 | 1.2:+79404980 | MS.gene060276:CDS |
| CCAAAATAAACTCTTGCTGT+TGG | 0.526318 | 1.2:-79405037 | None:intergenic |
| ATCCAGTAAATGGAGCAGTT+GGG | 0.536591 | 1.2:+79404874 | MS.gene060276:CDS |
| TCACTTATGTATGAAGCTGT+TGG | 0.539852 | 1.2:+79404840 | MS.gene060276:CDS |
| TGCATCCCTTTCTCAAAACT+CGG | 0.542491 | 1.2:-79404480 | None:intergenic |
| GAACAAGTGCTGAGAGGTAA+CGG | 0.548928 | 1.2:+79404939 | MS.gene060276:CDS |
| GATACAAAACCCACAAGCAC+AGG | 0.556238 | 1.2:+79404537 | MS.gene060276:CDS |
| TATAATAAAATGAGTTGCAA+CGG | 0.559441 | 1.2:+79404454 | None:intergenic |
| TTTGAGAAAGGGATGCAGTG+AGG | 0.560200 | 1.2:+79404486 | MS.gene060276:CDS |
| CAGCTTCATACATAAGTGAC+TGG | 0.569517 | 1.2:-79404836 | None:intergenic |
| GTAATGAAGAAGCACAATCT+GGG | 0.578649 | 1.2:+79405069 | MS.gene060276:CDS |
| ATACATGATCTTTGGTTGGG+AGG | 0.585857 | 1.2:-79404628 | None:intergenic |
| AGTACTCTAGAGTGGTGAGA+AGG | 0.591970 | 1.2:+79405102 | MS.gene060276:CDS |
| GGCTGTTATGGACTGGAAAG+TGG | 0.603769 | 1.2:+79404895 | MS.gene060276:CDS |
| GAAAAGACATGAGGGTGGCA+CGG | 0.613200 | 1.2:-79404594 | None:intergenic |
| CGTGCGGAGACACGAGTCTG+AGG | 0.614177 | 1.2:+79405149 | MS.gene060276:CDS |
| GCAGTTGGGCTGTTATGGAC+TGG | 0.615948 | 1.2:+79404888 | MS.gene060276:CDS |
| CCACACCTAACAACTGATCA+GGG | 0.634962 | 1.2:-79404980 | None:intergenic |
| GATTTCACAGTACTCTAGAG+TGG | 0.637833 | 1.2:+79405094 | MS.gene060276:CDS |
| AAGTGGCAATTCTGTCAACT+TGG | 0.652925 | 1.2:+79404912 | MS.gene060276:CDS |
| CCAAGCAGAAAAGCAACGTG+CGG | 0.655633 | 1.2:+79405133 | MS.gene060276:CDS |
| CAGGTGAAAGAAAAGACATG+AGG | 0.660626 | 1.2:-79404603 | None:intergenic |
| AGGTGAAAGAAAAGACATGA+GGG | 0.666800 | 1.2:-79404602 | None:intergenic |
| TAACAACTGATCAGGGAGTG+CGG | 0.677605 | 1.2:-79404973 | None:intergenic |
| TGCTACGAGATTGTCTAACG+TGG | 0.685370 | 1.2:+79404515 | MS.gene060276:CDS |
| GAACTTGGTGACAAAGACAG+TGG | 0.712149 | 1.2:-79404566 | None:intergenic |
| TGAAAGAAAAGACATGAGGG+TGG | 0.725920 | 1.2:-79404599 | None:intergenic |
| GGTGTTGAACAAGTGCTGAG+AGG | 0.756300 | 1.2:+79404933 | MS.gene060276:CDS |
| CAAGTGCTGAGAGGTAACGG+CGG | 0.757236 | 1.2:+79404942 | MS.gene060276:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTGTATGTATATATAT+AGG | + | chr1.2:79404733-79404752 | MS.gene060276:intron | 10.0% |
| !! | ATTCAATTACTCTATATTAC+AGG | + | chr1.2:79404807-79404826 | MS.gene060276:intron | 20.0% |
| !!! | ACAAATTAGTTTGATGTATT+TGG | - | chr1.2:79404721-79404740 | None:intergenic | 20.0% |
| ! | AGAACTATAAATCCAGTAAA+TGG | + | chr1.2:79404864-79404883 | MS.gene060276:CDS | 25.0% |
| ! | TTTATGACATACATGATCTT+TGG | - | chr1.2:79404639-79404658 | None:intergenic | 25.0% |
| !! | CAACAGCAAGAGTTTATTTT+GGG | + | chr1.2:79405038-79405057 | MS.gene060276:CDS | 30.0% |
| AGGTGAAAGAAAAGACATGA+GGG | - | chr1.2:79404605-79404624 | None:intergenic | 35.0% | |
| CACTTATGTATGAAGCTGTT+GGG | + | chr1.2:79404841-79404860 | MS.gene060276:CDS | 35.0% | |
| GACATACATGATCTTTGGTT+GGG | - | chr1.2:79404634-79404653 | None:intergenic | 35.0% | |
| TCACTTATGTATGAAGCTGT+TGG | + | chr1.2:79404840-79404859 | MS.gene060276:CDS | 35.0% | |
| TGACATACATGATCTTTGGT+TGG | - | chr1.2:79404635-79404654 | None:intergenic | 35.0% | |
| ! | CCAAAATAAACTCTTGCTGT+TGG | - | chr1.2:79405040-79405059 | None:intergenic | 35.0% |
| ! | CCAACAGCAAGAGTTTATTT+TGG | + | chr1.2:79405037-79405056 | MS.gene060276:CDS | 35.0% |
| ! | GTAATGAAGAAGCACAATCT+GGG | + | chr1.2:79405069-79405088 | MS.gene060276:CDS | 35.0% |
| ! | TGTAATGAAGAAGCACAATC+TGG | + | chr1.2:79405068-79405087 | MS.gene060276:CDS | 35.0% |
| AAGTGGCAATTCTGTCAACT+TGG | + | chr1.2:79404912-79404931 | MS.gene060276:CDS | 40.0% | |
| AATCCAGTAAATGGAGCAGT+TGG | + | chr1.2:79404873-79404892 | MS.gene060276:CDS | 40.0% | |
| ATACATGATCTTTGGTTGGG+AGG | - | chr1.2:79404631-79404650 | None:intergenic | 40.0% | |
| ATCCAGTAAATGGAGCAGTT+GGG | + | chr1.2:79404874-79404893 | MS.gene060276:CDS | 40.0% | |
| CAGCTTCATACATAAGTGAC+TGG | - | chr1.2:79404839-79404858 | None:intergenic | 40.0% | |
| CAGGTGAAAGAAAAGACATG+AGG | - | chr1.2:79404606-79404625 | None:intergenic | 40.0% | |
| GTCTTTGTCACCAAGTTCTT+CGG | + | chr1.2:79404571-79404590 | MS.gene060276:CDS | 40.0% | |
| TGAAAGAAAAGACATGAGGG+TGG | - | chr1.2:79404602-79404621 | None:intergenic | 40.0% | |
| TGCATCCCTTTCTCAAAACT+CGG | - | chr1.2:79404483-79404502 | None:intergenic | 40.0% | |
| ! | GATTTCACAGTACTCTAGAG+TGG | + | chr1.2:79405094-79405113 | MS.gene060276:CDS | 40.0% |
| CCACACCTAACAACTGATCA+GGG | - | chr1.2:79404983-79405002 | None:intergenic | 45.0% | |
| GATACAAAACCCACAAGCAC+AGG | + | chr1.2:79404537-79404556 | MS.gene060276:CDS | 45.0% | |
| TAACAACTGATCAGGGAGTG+CGG | - | chr1.2:79404976-79404995 | None:intergenic | 45.0% | |
| TCCACACCTAACAACTGATC+AGG | - | chr1.2:79404984-79405003 | None:intergenic | 45.0% | |
| TGCTACGAGATTGTCTAACG+TGG | + | chr1.2:79404515-79404534 | MS.gene060276:CDS | 45.0% | |
| TTTGAGAAAGGGATGCAGTG+AGG | + | chr1.2:79404486-79404505 | MS.gene060276:CDS | 45.0% | |
| ! | GAACTTGGTGACAAAGACAG+TGG | - | chr1.2:79404569-79404588 | None:intergenic | 45.0% |
| !! | AGTACTCTAGAGTGGTGAGA+AGG | + | chr1.2:79405102-79405121 | MS.gene060276:CDS | 45.0% |
| !! | GAACAAGTGCTGAGAGGTAA+CGG | + | chr1.2:79404939-79404958 | MS.gene060276:CDS | 45.0% |
| !!! | GGTTGCCGAGTTTTGAGAAA+GGG | + | chr1.2:79404475-79404494 | MS.gene060276:CDS | 45.0% |
| AGCCCAACTGCTCCATTTAC+TGG | - | chr1.2:79404879-79404898 | None:intergenic | 50.0% | |
| ATGGAGCAGTTGGGCTGTTA+TGG | + | chr1.2:79404883-79404902 | MS.gene060276:CDS | 50.0% | |
| CCAAGCAGAAAAGCAACGTG+CGG | + | chr1.2:79405133-79405152 | MS.gene060276:CDS | 50.0% | |
| CCCTGATCAGTTGTTAGGTG+TGG | + | chr1.2:79404980-79404999 | MS.gene060276:CDS | 50.0% | |
| GAAAAGACATGAGGGTGGCA+CGG | - | chr1.2:79404597-79404616 | None:intergenic | 50.0% | |
| GATCTTTGGTTGGGAGGAAC+AGG | - | chr1.2:79404625-79404644 | None:intergenic | 50.0% | |
| GCACTCCCTGATCAGTTGTT+AGG | + | chr1.2:79404975-79404994 | MS.gene060276:CDS | 50.0% | |
| !! | GGCTGTTATGGACTGGAAAG+TGG | + | chr1.2:79404895-79404914 | MS.gene060276:CDS | 50.0% |
| !! | GGTGTTGAACAAGTGCTGAG+AGG | + | chr1.2:79404933-79404952 | MS.gene060276:CDS | 50.0% |
| !!! | CGGTTGCCGAGTTTTGAGAA+AGG | + | chr1.2:79404474-79404493 | MS.gene060276:CDS | 50.0% |
| GCAGTTGGGCTGTTATGGAC+TGG | + | chr1.2:79404888-79404907 | MS.gene060276:CDS | 55.0% | |
| ! | TGGCGTTAGCCTGTGCTTGT+GGG | - | chr1.2:79404549-79404568 | None:intergenic | 55.0% |
| !! | CAAGTGCTGAGAGGTAACGG+CGG | + | chr1.2:79404942-79404961 | MS.gene060276:CDS | 55.0% |
| !!! | CCGCACGTTGCTTTTCTGCT+TGG | - | chr1.2:79405136-79405155 | None:intergenic | 55.0% |
| ! | GTGGCGTTAGCCTGTGCTTG+TGG | - | chr1.2:79404550-79404569 | None:intergenic | 60.0% |
| GGTGGCACGGCCGAAGAACT+TGG | - | chr1.2:79404584-79404603 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 79404463 | 79405169 | 79404463 | ID=MS.gene060276 |
| chr1.2 | mRNA | 79404463 | 79405169 | 79404463 | ID=MS.gene060276.t1;Parent=MS.gene060276 |
| chr1.2 | exon | 79404463 | 79404646 | 79404463 | ID=MS.gene060276.t1.exon1;Parent=MS.gene060276.t1 |
| chr1.2 | CDS | 79404463 | 79404646 | 79404463 | ID=cds.MS.gene060276.t1;Parent=MS.gene060276.t1 |
| chr1.2 | exon | 79404829 | 79405169 | 79404829 | ID=MS.gene060276.t1.exon2;Parent=MS.gene060276.t1 |
| chr1.2 | CDS | 79404829 | 79405169 | 79404829 | ID=cds.MS.gene060276.t1;Parent=MS.gene060276.t1 |
| Gene Sequence |
| Protein sequence |
