AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71633

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71633.t1 MTR_1g008290 34.921 126 79 1 103 228 22 144 3.05e-22 94.0
MS.gene71633.t1 MTR_4g035820 25.729 377 240 11 29 387 70 424 1.39e-19 93.6
MS.gene71633.t1 MTR_6g051425 25.926 216 143 5 37 240 20 230 1.93e-17 84.7
MS.gene71633.t1 MTR_3g006370 37.383 107 60 2 33 134 49 153 2.58e-16 79.0
MS.gene71633.t1 MTR_4g067290 29.630 135 91 2 3 136 28 159 8.93e-15 74.7
MS.gene71633.t1 MTR_4g067290 29.630 135 91 2 3 136 28 159 9.15e-15 74.7
MS.gene71633.t1 MTR_4g067290 29.630 135 91 2 3 136 48 179 1.39e-14 74.7
MS.gene71633.t1 MTR_4g067290 29.630 135 91 2 3 136 48 179 1.48e-14 74.7
MS.gene71633.t1 MTR_4g067290 29.630 135 91 2 3 136 46 177 1.50e-14 74.3
MS.gene71633.t1 MTR_4g067290 29.630 135 91 2 3 136 88 219 1.80e-14 75.1
MS.gene71633.t1 MTR_1g070355 28.910 211 135 7 39 240 79 283 6.20e-13 72.4
MS.gene71633.t1 MTR_6g022070 31.111 135 90 3 244 376 34 167 2.72e-12 68.9
MS.gene71633.t1 MTR_2g098880 31.250 112 72 2 28 134 54 165 6.95e-12 66.6
MS.gene71633.t1 MTR_2g098880 31.250 112 72 2 28 134 66 177 7.56e-12 66.6
MS.gene71633.t1 MTR_8g465780 29.231 130 87 3 283 408 1 129 5.50e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71633.t1 AT4G15090 58.028 436 156 3 241 653 381 812 5.14e-176 522
MS.gene71633.t1 AT4G15090 76.577 222 49 2 22 240 39 260 2.38e-108 347
MS.gene71633.t1 AT4G15090 58.028 436 156 3 241 653 381 812 5.14e-176 522
MS.gene71633.t1 AT4G15090 76.577 222 49 2 22 240 39 260 2.38e-108 347
MS.gene71633.t1 AT4G15090 58.028 436 156 3 241 653 381 812 5.14e-176 522
MS.gene71633.t1 AT4G15090 76.577 222 49 2 22 240 39 260 2.38e-108 347
MS.gene71633.t1 AT4G15090 58.028 436 156 3 241 653 381 812 5.14e-176 522
MS.gene71633.t1 AT4G15090 76.577 222 49 2 22 240 39 260 2.38e-108 347
MS.gene71633.t1 AT4G15090 58.028 436 156 3 241 653 381 812 5.14e-176 522
MS.gene71633.t1 AT4G15090 76.577 222 49 2 22 240 39 260 2.38e-108 347
MS.gene71633.t1 AT3G22170 48.673 452 204 5 241 666 390 839 1.74e-134 416
MS.gene71633.t1 AT3G22170 43.777 233 93 3 23 240 60 269 2.53e-57 208
MS.gene71633.t1 AT3G22170 48.673 452 204 5 241 666 390 839 1.74e-134 416
MS.gene71633.t1 AT3G22170 43.777 233 93 3 23 240 60 269 2.53e-57 208
MS.gene71633.t1 AT1G76320 44.479 317 170 2 240 552 317 631 4.91e-94 305
MS.gene71633.t1 AT1G76320 44.335 203 107 2 38 240 1 197 1.15e-50 187
MS.gene71633.t1 AT1G76320 44.340 318 171 2 239 552 316 631 9.11e-94 306
MS.gene71633.t1 AT1G76320 44.335 203 107 2 38 240 1 197 1.98e-50 187
MS.gene71633.t1 AT1G76320 44.340 318 171 2 239 552 316 631 9.43e-94 306
MS.gene71633.t1 AT1G76320 44.335 203 107 2 38 240 1 197 2.06e-50 187
MS.gene71633.t1 AT1G76320 44.340 318 171 2 239 552 316 631 9.43e-94 306
MS.gene71633.t1 AT1G76320 44.335 203 107 2 38 240 1 197 2.06e-50 187
MS.gene71633.t1 AT4G19990 44.501 391 198 5 242 613 273 663 1.46e-85 283
MS.gene71633.t1 AT4G19990 33.696 184 72 2 72 240 2 150 2.73e-22 102
MS.gene71633.t1 AT4G19990 44.501 391 198 5 242 613 330 720 2.20e-85 284
MS.gene71633.t1 AT4G19990 37.778 225 90 2 31 240 18 207 1.77e-36 146
MS.gene71633.t1 AT4G19990 44.501 391 198 5 242 613 330 720 2.20e-85 284
MS.gene71633.t1 AT4G19990 37.778 225 90 2 31 240 18 207 1.77e-36 146
MS.gene71633.t1 AT4G19990 44.501 391 198 5 242 613 330 720 2.62e-85 284
MS.gene71633.t1 AT4G19990 38.938 226 86 4 31 240 18 207 1.86e-36 146
MS.gene71633.t1 AT4G19990 44.501 391 198 5 242 613 330 720 3.04e-85 284
MS.gene71633.t1 AT4G19990 37.778 225 90 2 31 240 18 207 2.95e-36 146
MS.gene71633.t1 AT4G19990 44.501 391 198 5 242 613 330 720 3.04e-85 284
MS.gene71633.t1 AT4G19990 37.778 225 90 2 31 240 18 207 2.95e-36 146
MS.gene71633.t1 AT4G19990 44.501 391 198 5 242 613 344 734 3.54e-85 284
MS.gene71633.t1 AT4G19990 37.778 225 90 2 31 240 32 221 2.19e-36 146
MS.gene71633.t1 AT2G32250 36.930 417 235 5 243 635 286 698 1.01e-77 263
MS.gene71633.t1 AT2G32250 36.946 203 88 3 38 240 1 163 8.81e-28 119
MS.gene71633.t1 AT2G32250 36.930 417 235 5 243 635 286 698 1.09e-77 263
MS.gene71633.t1 AT2G32250 36.946 203 88 3 38 240 1 163 9.58e-28 119
MS.gene71633.t1 AT2G32250 37.019 416 234 5 244 635 328 739 1.51e-77 263
MS.gene71633.t1 AT2G32250 37.561 205 88 3 36 240 40 204 2.54e-29 124
MS.gene71633.t1 AT2G32250 37.019 416 234 5 244 635 328 739 2.03e-77 264
MS.gene71633.t1 AT2G32250 37.500 208 90 3 33 240 37 204 2.69e-29 124
MS.gene71633.t1 AT2G32250 37.019 416 234 5 244 635 328 739 2.03e-77 264
MS.gene71633.t1 AT2G32250 37.500 208 90 3 33 240 37 204 2.69e-29 124
MS.gene71633.t1 AT2G32250 37.019 416 234 5 244 635 328 739 2.03e-77 264
MS.gene71633.t1 AT2G32250 37.500 208 90 3 33 240 37 204 2.69e-29 124
MS.gene71633.t1 AT2G32250 37.019 416 234 5 244 635 328 739 2.29e-77 263
MS.gene71633.t1 AT2G32250 37.500 208 90 3 33 240 37 204 2.87e-29 124
MS.gene71633.t1 AT2G32250 37.019 416 234 5 244 635 328 739 2.29e-77 263
MS.gene71633.t1 AT2G32250 37.500 208 90 3 33 240 37 204 2.87e-29 124
MS.gene71633.t1 AT4G38180 34.539 304 192 3 232 528 395 698 2.07e-54 199
MS.gene71633.t1 AT4G38180 34.259 216 135 3 31 240 70 284 1.49e-30 128
MS.gene71633.t1 AT2G27110 33.219 292 184 4 241 526 207 493 5.60e-48 180
MS.gene71633.t1 AT1G52520 34.539 304 179 5 233 520 393 692 6.40e-48 180
MS.gene71633.t1 AT1G52520 31.308 214 132 6 34 244 85 286 7.92e-22 100
MS.gene71633.t1 AT2G27110 33.219 292 184 4 241 526 352 638 1.24e-47 180
MS.gene71633.t1 AT2G27110 27.751 209 125 5 33 240 48 231 3.39e-17 86.3
MS.gene71633.t1 AT2G27110 33.219 292 184 4 241 526 352 638 1.24e-47 180
MS.gene71633.t1 AT2G27110 27.751 209 125 5 33 240 48 231 3.39e-17 86.3
MS.gene71633.t1 AT4G38170 30.211 331 208 5 232 548 160 481 4.16e-46 172
MS.gene71633.t1 AT1G80010 31.884 276 173 8 259 524 444 714 3.14e-37 149
MS.gene71633.t1 AT1G80010 29.767 215 140 4 34 240 95 306 1.94e-19 93.6
MS.gene71633.t1 AT5G18960 31.276 243 163 2 229 470 490 729 4.26e-34 139
MS.gene71633.t1 AT5G18960 31.276 243 163 2 229 470 490 729 4.26e-34 139
MS.gene71633.t1 AT3G06250 31.746 252 156 5 225 470 464 705 4.38e-34 139
MS.gene71633.t1 AT3G06250 33.929 112 63 1 22 133 16 116 6.37e-11 66.2
MS.gene71633.t1 AT3G06250 31.746 252 156 5 225 470 464 705 4.38e-34 139
MS.gene71633.t1 AT3G06250 33.929 112 63 1 22 133 16 116 6.37e-11 66.2
MS.gene71633.t1 AT3G06250 31.746 252 156 5 225 470 464 705 4.38e-34 139
MS.gene71633.t1 AT3G06250 33.929 112 63 1 22 133 16 116 6.37e-11 66.2
MS.gene71633.t1 AT1G10240 30.131 229 151 3 259 482 401 625 4.58e-26 114
MS.gene71633.t1 AT1G10240 27.928 222 138 8 34 240 48 262 9.16e-12 68.9
MS.gene71633.t1 AT1G10240 30.131 229 151 3 259 482 401 625 4.58e-26 114
MS.gene71633.t1 AT1G10240 27.928 222 138 8 34 240 48 262 9.16e-12 68.9
MS.gene71633.t1 AT5G28530 30.594 219 125 7 259 468 353 553 8.13e-19 91.3
MS.gene71633.t1 AT5G28530 29.224 219 132 6 259 468 353 557 1.27e-18 90.5
MS.gene71633.t1 AT5G28530 29.536 237 139 8 241 468 399 616 1.30e-18 90.5
MS.gene71633.t1 AT5G28530 29.536 237 139 8 241 468 399 616 1.30e-18 90.5
MS.gene71633.t1 AT5G28530 28.270 237 146 7 241 468 399 620 1.70e-18 90.5
MS.gene71633.t1 AT2G43280 36.975 119 66 3 29 140 17 133 5.89e-16 77.4
MS.gene71633.t1 AT4G12850 39.604 101 60 1 33 133 10 109 4.61e-15 73.2
MS.gene71633.t1 AT4G12850 39.604 101 60 1 33 133 10 109 6.17e-15 72.8
MS.gene71633.t1 AT3G59470 34.286 105 68 1 33 136 69 173 1.21e-14 74.7
MS.gene71633.t1 AT3G59470 34.286 105 68 1 33 136 69 173 1.21e-14 74.7
MS.gene71633.t1 AT3G59470 34.286 105 68 1 33 136 81 185 1.28e-14 75.1
MS.gene71633.t1 AT4G12850 39.604 101 60 1 33 133 10 109 2.35e-14 72.4
MS.gene71633.t1 AT3G07500 30.323 155 98 2 27 179 26 172 3.19e-14 72.8
MS.gene71633.t1 AT2G43280 35.354 99 55 3 49 140 2 98 3.79e-11 62.8

Find 127 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTACAGGTTCCCTAATTT+TGG 0.145787 1.4:+82407634 None:intergenic
ACTGTTTCACAAACTCTTTA+AGG 0.204134 1.4:+82408062 None:intergenic
AACGTTTAAGAAATATATTT+TGG 0.206444 1.4:-82408699 MS.gene71633:CDS
TCGGATGCAATTGCCCTTAA+TGG 0.236596 1.4:-82406984 MS.gene71633:CDS
ATTGCCCTTAATGGATATTA+TGG 0.237741 1.4:-82406975 MS.gene71633:CDS
GGACCAAAGATGCCAAAATT+AGG 0.253094 1.4:-82407646 MS.gene71633:CDS
TTTGGCATCAATAAATTCTT+TGG 0.254194 1.4:+82409182 None:intergenic
CATTTCAACATACATCTTTC+GGG 0.258771 1.4:+82408903 None:intergenic
TGTCGATTGTTCGAATATAA+AGG 0.270665 1.4:-82407749 MS.gene71633:CDS
GAGTACCATAATATCCATTA+AGG 0.276199 1.4:+82406970 None:intergenic
TCAAGAGTATGCCAAATCTA+TGG 0.278613 1.4:-82409247 MS.gene71633:CDS
ATACCTAGAGCACGCGAATT+TGG 0.279686 1.4:+82409164 None:intergenic
GCATGTACAATTGAACTTAA+TGG 0.289292 1.4:-82406834 MS.gene71633:CDS
ACATTTCAACATACATCTTT+CGG 0.295052 1.4:+82408902 None:intergenic
AAGAAAGCTATGCCCCTCTT+AGG 0.295788 1.4:+82409342 None:intergenic
CTGTTTCACAAACTCTTTAA+GGG 0.296391 1.4:+82408063 None:intergenic
TATCGTCCGACAACCACTTT+TGG 0.332110 1.4:-82406443 MS.gene71633:CDS
AAAATGCAATTCTTAAACTT+TGG 0.338533 1.4:+82408301 None:intergenic
AAAATGTTCCATTTCCCATC+TGG 0.352283 1.4:+82409051 None:intergenic
AACGACAATGGTGCAGAAAC+TGG 0.362647 1.4:-82407449 MS.gene71633:CDS
GTTTGACAGAGGGCAGTATC+TGG 0.368111 1.4:-82408821 MS.gene71633:CDS
ATAGGTTAATTCTTCTGAAT+AGG 0.384277 1.4:-82408039 MS.gene71633:CDS
GTTGTGTTCCTCTTCTTACT+TGG 0.388273 1.4:+82407368 None:intergenic
AGAAAGCTATGCCCCTCTTA+GGG 0.402896 1.4:+82409343 None:intergenic
ATCTATAGAATAGAAGAAGT+TGG 0.403398 1.4:+82408735 None:intergenic
AAGAGTTTGTGAAACAGTAT+AGG 0.409635 1.4:-82408057 MS.gene71633:CDS
AGTGGTGAATCCCATAGATT+TGG 0.415385 1.4:+82409236 None:intergenic
CAGAGGGCAGTATCTGGCTT+TGG 0.416018 1.4:-82408815 MS.gene71633:CDS
CACTGGTTGCTTCCCCTAAG+AGG 0.417880 1.4:-82409356 MS.gene71633:CDS
GACCAAAGATGCCAAAATTA+GGG 0.426826 1.4:-82407645 MS.gene71633:CDS
TTGAACTACATGATGATGTT+TGG 0.426842 1.4:-82408222 MS.gene71633:CDS
AAATGTTCCATTTCCCATCT+GGG 0.441346 1.4:+82409052 None:intergenic
TTGCTGTGAAAGTGTGGATC+AGG 0.445132 1.4:+82406283 None:intergenic
TCAAAACATTGGGTCAATCA+TGG 0.447302 1.4:-82408860 MS.gene71633:CDS
CTTTGTGTCCTATCTACTAC+AGG 0.448746 1.4:+82407620 None:intergenic
GTTGAAGTGTTGGGTGTAGC+TGG 0.449517 1.4:-82407893 MS.gene71633:CDS
GTTCATGTAGGGAGGGCAAT+TGG 0.449744 1.4:-82406468 MS.gene71633:intron
TCTATAGAATAGAAGAAGTT+GGG 0.462776 1.4:+82408736 None:intergenic
GGGAGATGGGCAAGTTATGC+TGG 0.466240 1.4:-82408788 MS.gene71633:CDS
GCAGTATCTGGCTTTGGATG+AGG 0.466634 1.4:-82408809 MS.gene71633:CDS
TCAAGATACCCTGCAGCAAA+TGG 0.469548 1.4:-82407198 MS.gene71633:intron
ACTTAAATCTCCTTCACCTT+GGG 0.470138 1.4:-82407966 MS.gene71633:CDS
GTTGAAATGTCTAGACAATC+TGG 0.471543 1.4:-82408889 MS.gene71633:CDS
GAAATTCCAAGTTGAAGTGT+TGG 0.475026 1.4:-82407903 MS.gene71633:CDS
ATGCCAAATTCGCGTGCTCT+AGG 0.479668 1.4:-82409167 MS.gene71633:CDS
AGGGGCATAGCTTTCTTTGA+AGG 0.480618 1.4:-82409336 MS.gene71633:CDS
ACTGGTTGCTTCCCCTAAGA+GGG 0.487153 1.4:-82409355 MS.gene71633:CDS
AAATTCCAAGTTGAAGTGTT+GGG 0.488182 1.4:-82407902 MS.gene71633:CDS
TTAGGGAACCTGTAGTAGAT+AGG 0.488222 1.4:-82407628 MS.gene71633:CDS
CTGGTGGCTGTCAAAACATT+GGG 0.488703 1.4:-82408870 MS.gene71633:CDS
GTATTTCAAGCATATTCAAA+AGG 0.489011 1.4:-82408764 MS.gene71633:CDS
GTCAAACTGTTCATCTGTCC+AGG 0.491245 1.4:+82408273 None:intergenic
GTGTAGCTGGATGTCAATCT+AGG 0.493252 1.4:-82407880 MS.gene71633:CDS
TTCACTCATTGTACGAAGAC+CGG 0.494147 1.4:-82408198 MS.gene71633:CDS
CTGGCTTTGGATGAGGGAGA+TGG 0.494760 1.4:-82408802 MS.gene71633:CDS
GTTTCAAATTCAATGCCGTT+AGG 0.501314 1.4:+82409294 None:intergenic
CACTTAAATCTCCTTCACCT+TGG 0.501635 1.4:-82407967 MS.gene71633:CDS
CGAAAATGATATGCCAAAGC+TGG 0.503037 1.4:+82408994 None:intergenic
AGTACCATAATATCCATTAA+GGG 0.503128 1.4:+82406971 None:intergenic
AGTATTGCGTTGAGTGAAGA+AGG 0.512046 1.4:-82407554 MS.gene71633:CDS
GGTGAAGGAGATTTAAGTGC+AGG 0.515427 1.4:+82407971 None:intergenic
GCATCACACGGGTGGTGCAC+TGG 0.518829 1.4:-82409373 MS.gene71633:CDS
CAGTATCTGGCTTTGGATGA+GGG 0.524524 1.4:-82408808 MS.gene71633:CDS
CAAGAGTATGCCAAATCTAT+GGG 0.524696 1.4:-82409246 MS.gene71633:CDS
CAAAACATTGGGTCAATCAT+GGG 0.529150 1.4:-82408859 MS.gene71633:CDS
GGGGCATAGCTTTCTTTGAA+GGG 0.530599 1.4:-82409335 MS.gene71633:CDS
TCTTGAGCGTCGACAAGTAC+AGG 0.531111 1.4:+82407215 None:intergenic
TCAACACAGCATGCAAGGCC+TGG 0.531717 1.4:-82406783 MS.gene71633:intron
CACTTGAACAACCGCAACGT+TGG 0.534327 1.4:+82407696 None:intergenic
TGGCTTTGGATGAGGGAGAT+GGG 0.535356 1.4:-82408801 MS.gene71633:CDS
AGCTTTCTTTGAAGGGGACA+CGG 0.537323 1.4:-82409328 MS.gene71633:CDS
AAACACAAACCATTTGCTGC+AGG 0.538027 1.4:+82407189 None:intergenic
CTGAAGGCTATATCCAAAAG+TGG 0.546535 1.4:+82406430 None:intergenic
CTACACCCAACACTTCAACT+TGG 0.546709 1.4:+82407897 None:intergenic
TCTGGTGGCTGTCAAAACAT+TGG 0.549465 1.4:-82408871 MS.gene71633:CDS
CTCATTATATCATGAAGAGA+TGG 0.550080 1.4:-82407667 MS.gene71633:CDS
AAATTCGCGTGCTCTAGGTA+TGG 0.552712 1.4:-82409162 MS.gene71633:CDS
CACTCAATGCATGTCTGCAA+AGG 0.555274 1.4:+82407723 None:intergenic
CTATATCCAAAAGTGGTTGT+CGG 0.558538 1.4:+82406437 None:intergenic
TGTCAATCTAGGACAGAGGT+TGG 0.561454 1.4:-82407869 MS.gene71633:CDS
GAACACAACTAGGAAAAGAA+AGG 0.562569 1.4:-82407354 MS.gene71633:intron
TCAGCAGAATGTGCAAGGAC+TGG 0.567200 1.4:-82406949 MS.gene71633:intron
GTTAATCAACACAGCATGCA+AGG 0.568293 1.4:-82406788 MS.gene71633:CDS
GTAAGAAGAGGAACACAACT+AGG 0.569051 1.4:-82407364 MS.gene71633:CDS
CTTCAAAACCAAGTAAGAAG+AGG 0.570332 1.4:-82407376 MS.gene71633:CDS
TTTCAAGCATATTCAAAAGG+AGG 0.574379 1.4:-82408761 MS.gene71633:CDS
CTCTTCATGATATAATGAGA+TGG 0.574857 1.4:+82407671 None:intergenic
GTAAATAACTCTAACGACAA+TGG 0.578738 1.4:-82407461 MS.gene71633:CDS
GGGCATAGCTTTCTTTGAAG+GGG 0.579841 1.4:-82409334 MS.gene71633:CDS
GGACACAAAGGATACAAACT+AGG 0.580462 1.4:-82407607 MS.gene71633:CDS
GAACTGTTGCTGTGAAAGTG+TGG 0.585833 1.4:+82406277 None:intergenic
GGTGCAGAAACTGGCAACAA+TGG 0.585898 1.4:-82407440 MS.gene71633:CDS
TCAAATTCAATGCCGTTAGG+TGG 0.590422 1.4:+82409297 None:intergenic
CACACATCATTATAACGTTG+AGG 0.592933 1.4:+82407584 None:intergenic
TCTAGGACAGAGGTTGGAGA+TGG 0.594905 1.4:-82407863 MS.gene71633:CDS
AAAGTGGGTACCGACATACA+TGG 0.603217 1.4:-82408173 MS.gene71633:CDS
AACACAAACCATTTGCTGCA+GGG 0.603531 1.4:+82407190 None:intergenic
GAAAGCTATGCCCCTCTTAG+GGG 0.607330 1.4:+82409344 None:intergenic
GGTACTCAGCAGAATGTGCA+AGG 0.609447 1.4:-82406954 MS.gene71633:CDS
CGAGTTATCAGTTTGACAGA+GGG 0.612110 1.4:-82408831 MS.gene71633:CDS
TTAATGGAGCCACCTCATGA+TGG 0.620785 1.4:-82406818 MS.gene71633:CDS
AACAGTTTGACATGAGATGG+TGG 0.621739 1.4:-82408261 MS.gene71633:CDS
CTGGTTGCTTCCCCTAAGAG+GGG 0.625137 1.4:-82409354 MS.gene71633:CDS
GGAGTAACACCTGAGTCTGA+TGG 0.629153 1.4:-82409141 MS.gene71633:CDS
ACTGTCGGAACGTTGAGGTG+TGG 0.629360 1.4:+82408126 None:intergenic
CTGCTCCTACCATCAGACTC+AGG 0.632022 1.4:+82409132 None:intergenic
AAAGAAAGAGTTCACACTGT+CGG 0.634665 1.4:+82408111 None:intergenic
TCTTCTGAATAGGTATGATG+AGG 0.638772 1.4:-82408029 MS.gene71633:CDS
TTGAGTGTTCTCCAACGTTG+CGG 0.639483 1.4:-82407707 MS.gene71633:CDS
GTCAATGAAGAGCATCACAC+GGG 0.641639 1.4:-82409384 MS.gene71633:CDS
ATGAACAGTTTGACATGAGA+TGG 0.648940 1.4:-82408264 MS.gene71633:CDS
TGGATGTCAATCTAGGACAG+AGG 0.649330 1.4:-82407873 MS.gene71633:CDS
AAGTGGGTACCGACATACAT+GGG 0.651329 1.4:-82408172 MS.gene71633:CDS
AAGACATCTCCCATGTATGT+CGG 0.652653 1.4:+82408163 None:intergenic
TATAGTTCAAGATTACGAGA+AGG 0.655112 1.4:-82407825 MS.gene71633:CDS
TTCACACTGTCGGAACGTTG+AGG 0.659741 1.4:+82408121 None:intergenic
CTATTCTATAGATTTAAACG+AGG 0.660746 1.4:-82408725 MS.gene71633:CDS
ACATAGTAACCATCATGAGG+TGG 0.662349 1.4:+82406809 None:intergenic
ACGAGTTATCAGTTTGACAG+AGG 0.665307 1.4:-82408832 MS.gene71633:CDS
TGTCAATGAAGAGCATCACA+CGG 0.667976 1.4:-82409385 MS.gene71633:CDS
AATGAAGAGCATCACACGGG+TGG 0.670295 1.4:-82409381 MS.gene71633:CDS
TAACACCTGAGTCTGATGGT+AGG 0.675623 1.4:-82409137 MS.gene71633:CDS
CTGTAGTAGATAGGACACAA+AGG 0.687200 1.4:-82407619 MS.gene71633:CDS
TTAACATAGTAACCATCATG+AGG 0.693243 1.4:+82406806 None:intergenic
TTGGAACGAGTTGAGCTCAG+AGG 0.703393 1.4:-82407783 MS.gene71633:CDS
ACAGTTTGACATGAGATGGT+GGG 0.706939 1.4:-82408260 MS.gene71633:CDS
GAAATGTCTAGACAATCTGG+TGG 0.763154 1.4:-82408886 MS.gene71633:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAATTATTAATTATAA+AGG - chr1.4:82408948-82408967 MS.gene71633:CDS 0.0%
!!! AACGTTTAAGAAATATATTT+TGG - chr1.4:82406961-82406980 MS.gene71633:CDS 15.0%
!!! ACGTTTAAGAAATATATTTT+GGG - chr1.4:82406962-82406981 MS.gene71633:CDS 15.0%
!!! GAACATTTTTGAATTTATAA+AGG - chr1.4:82406623-82406642 MS.gene71633:intron 15.0%
!! AAAATGCAATTCTTAAACTT+TGG + chr1.4:82407362-82407381 None:intergenic 20.0%
!! AGAAGATTATATTCATGATT+AGG + chr1.4:82408911-82408930 None:intergenic 20.0%
!! CTAATAAGTAACTAAGTATA+CGG + chr1.4:82409274-82409293 None:intergenic 20.0%
!! TAATTTCCCAATAATTTCAT+AGG + chr1.4:82409126-82409145 None:intergenic 20.0%
!! TACTTTCCTATGAAATTATT+GGG - chr1.4:82409117-82409136 MS.gene71633:CDS 20.0%
!! TTAGATTATACAAATAAGCA+TGG - chr1.4:82408519-82408538 MS.gene71633:intron 20.0%
!!! TGCTTAATAGATTGTAATTT+TGG - chr1.4:82408795-82408814 MS.gene71633:CDS 20.0%
! ACATTTCAACATACATCTTT+CGG + chr1.4:82406761-82406780 None:intergenic 25.0%
! AGAACATTGTAATCATTTGA+TGG + chr1.4:82408753-82408772 None:intergenic 25.0%
! AGTACCATAATATCCATTAA+GGG + chr1.4:82408692-82408711 None:intergenic 25.0%
! ATAGGTTAATTCTTCTGAAT+AGG - chr1.4:82407621-82407640 MS.gene71633:CDS 25.0%
! ATCTATAGAATAGAAGAAGT+TGG + chr1.4:82406928-82406947 None:intergenic 25.0%
! ATTGATATTCATATCGTTGT+AGG - chr1.4:82409020-82409039 MS.gene71633:CDS 25.0%
! CTATTCTATAGATTTAAACG+AGG - chr1.4:82406935-82406954 MS.gene71633:intron 25.0%
! GTACTTTCCTATGAAATTAT+TGG - chr1.4:82409116-82409135 MS.gene71633:CDS 25.0%
! GTATTTCAAGCATATTCAAA+AGG - chr1.4:82406896-82406915 MS.gene71633:intron 25.0%
! TACTTATTAGCATTTGAACT+TGG - chr1.4:82409284-82409303 MS.gene71633:CDS 25.0%
! TCTATAGAATAGAAGAAGTT+GGG + chr1.4:82406927-82406946 None:intergenic 25.0%
! TTGATATTCATATCGTTGTA+GGG - chr1.4:82409021-82409040 MS.gene71633:CDS 25.0%
! TTTGGCATCAATAAATTCTT+TGG + chr1.4:82406481-82406500 None:intergenic 25.0%
!! TGAATAGAATAATTTGTGCT+TGG - chr1.4:82409312-82409331 MS.gene71633:CDS 25.0%
!! TTTCTAGCATTTAAAAGATC+AGG - chr1.4:82408378-82408397 MS.gene71633:intron 25.0%
AAGAGTTTGTGAAACAGTAT+AGG - chr1.4:82407603-82407622 MS.gene71633:CDS 30.0%
ACTGTTTCACAAACTCTTTA+AGG + chr1.4:82407601-82407620 None:intergenic 30.0%
ATAGAAATGCAATTCTCAAG+AGG + chr1.4:82408554-82408573 None:intergenic 30.0%
CAGATCATGAATGAAAAAAG+AGG + chr1.4:82408497-82408516 None:intergenic 30.0%
CATTTCAACATACATCTTTC+GGG + chr1.4:82406760-82406779 None:intergenic 30.0%
CTCATTATATCATGAAGAGA+TGG - chr1.4:82407993-82408012 MS.gene71633:CDS 30.0%
CTCTTCATGATATAATGAGA+TGG + chr1.4:82407992-82408011 None:intergenic 30.0%
CTGTTTCACAAACTCTTTAA+GGG + chr1.4:82407600-82407619 None:intergenic 30.0%
GAGTACCATAATATCCATTA+AGG + chr1.4:82408693-82408712 None:intergenic 30.0%
GCATGTACAATTGAACTTAA+TGG - chr1.4:82408826-82408845 MS.gene71633:CDS 30.0%
GTAAATAACTCTAACGACAA+TGG - chr1.4:82408199-82408218 MS.gene71633:CDS 30.0%
TATAGTTCAAGATTACGAGA+AGG - chr1.4:82407835-82407854 MS.gene71633:CDS 30.0%
TGATACGAGAATCACTTTAT+AGG - chr1.4:82408405-82408424 MS.gene71633:intron 30.0%
TGATCAGTGACTATAACTTT+GGG + chr1.4:82407209-82407228 None:intergenic 30.0%
TTAACATAGTAACCATCATG+AGG + chr1.4:82408857-82408876 None:intergenic 30.0%
TTGATCAGTGACTATAACTT+TGG + chr1.4:82407210-82407229 None:intergenic 30.0%
TTGCACATAACCTTAATTGT+AGG - chr1.4:82409349-82409368 MS.gene71633:CDS 30.0%
TTTCAAGCATATTCAAAAGG+AGG - chr1.4:82406899-82406918 MS.gene71633:intron 30.0%
! AAATTCCAAGTTGAAGTGTT+GGG - chr1.4:82407758-82407777 MS.gene71633:CDS 30.0%
! ATCACAAAAGACAGATTTTC+AGG + chr1.4:82407317-82407336 None:intergenic 30.0%
! ATTGCCCTTAATGGATATTA+TGG - chr1.4:82408685-82408704 MS.gene71633:intron 30.0%
! CTTTTCTCTTTGTCACATAT+TGG - chr1.4:82407283-82407302 MS.gene71633:intron 30.0%
! TGTCGATTGTTCGAATATAA+AGG - chr1.4:82407911-82407930 MS.gene71633:CDS 30.0%
! TTGAACTACATGATGATGTT+TGG - chr1.4:82407438-82407457 MS.gene71633:CDS 30.0%
!! GATACAAGTTTGCTAACTAT+GGG - chr1.4:82409166-82409185 MS.gene71633:CDS 30.0%
!! TGATACAAGTTTGCTAACTA+TGG - chr1.4:82409165-82409184 MS.gene71633:CDS 30.0%
!!! AGAATTGCATTTTTAAGTCC+TGG - chr1.4:82407369-82407388 MS.gene71633:CDS 30.0%
!!! ATGAAGAGTTTTTGGTAACT+TGG - chr1.4:82407858-82407877 MS.gene71633:CDS 30.0%
!!! CTTATCGTTGCTTTTTATGT+AGG + chr1.4:82407048-82407067 None:intergenic 30.0%
!!! CTTTTAGGATAATCTTAGCT+CGG - chr1.4:82408657-82408676 MS.gene71633:intron 30.0%
AAAAGCAATGACGTACACTT+GGG + chr1.4:82407268-82407287 None:intergenic 35.0%
AAAATGTTCCATTTCCCATC+TGG + chr1.4:82406612-82406631 None:intergenic 35.0%
AAACTGCATCATTAAAGCTG+AGG + chr1.4:82407013-82407032 None:intergenic 35.0%
AAAGAAAGAGTTCACACTGT+CGG + chr1.4:82407552-82407571 None:intergenic 35.0%
AAATGCAATTCTCAAGAGGA+TGG + chr1.4:82408550-82408569 None:intergenic 35.0%
AAATGTTCCATTTCCCATCT+GGG + chr1.4:82406611-82406630 None:intergenic 35.0%
AATGCACATCCAAGCAATAT+AGG + chr1.4:82407113-82407132 None:intergenic 35.0%
ACATCCAAGCAATATAGGTT+GGG + chr1.4:82407108-82407127 None:intergenic 35.0%
ACTTAAATCTCCTTCACCTT+GGG - chr1.4:82407694-82407713 MS.gene71633:CDS 35.0%
ATGAACAGTTTGACATGAGA+TGG - chr1.4:82407396-82407415 MS.gene71633:CDS 35.0%
CAAAACATTGGGTCAATCAT+GGG - chr1.4:82406801-82406820 MS.gene71633:CDS 35.0%
CACACATCATTATAACGTTG+AGG + chr1.4:82408079-82408098 None:intergenic 35.0%
CTATATCCAAAAGTGGTTGT+CGG + chr1.4:82409226-82409245 None:intergenic 35.0%
CTCAGTAACCAAACAAAAGT+GGG + chr1.4:82407155-82407174 None:intergenic 35.0%
CTTCAAAACCAAGTAAGAAG+AGG - chr1.4:82408284-82408303 MS.gene71633:CDS 35.0%
GAACACAACTAGGAAAAGAA+AGG - chr1.4:82408306-82408325 MS.gene71633:CDS 35.0%
GACCAAAGATGCCAAAATTA+GGG - chr1.4:82408015-82408034 MS.gene71633:CDS 35.0%
GTTGAAATGTCTAGACAATC+TGG - chr1.4:82406771-82406790 MS.gene71633:intron 35.0%
GTTTCAAATTCAATGCCGTT+AGG + chr1.4:82406369-82406388 None:intergenic 35.0%
TAAGGTTATGTGCAAACAGA+CGG + chr1.4:82409344-82409363 None:intergenic 35.0%
TATATTGCTTGGATGTGCAT+TGG - chr1.4:82407112-82407131 MS.gene71633:intron 35.0%
TCAAAACATTGGGTCAATCA+TGG - chr1.4:82406800-82406819 MS.gene71633:CDS 35.0%
TCTTCTGAATAGGTATGATG+AGG - chr1.4:82407631-82407650 MS.gene71633:CDS 35.0%
TTCTTCAATTGCTTCCTTCA+AGG + chr1.4:82407240-82407259 None:intergenic 35.0%
! ACATGTGCTTTCGTTCTTTT+AGG - chr1.4:82408642-82408661 MS.gene71633:intron 35.0%
! CAAGAGTATGCCAAATCTAT+GGG - chr1.4:82406414-82406433 MS.gene71633:intron 35.0%
! GAAATTCCAAGTTGAAGTGT+TGG - chr1.4:82407757-82407776 MS.gene71633:CDS 35.0%
! GAACACAACAAAGCATTCAT+GGG - chr1.4:82409085-82409104 MS.gene71633:CDS 35.0%
! GCATATCATTTTCGGATTCA+TGG - chr1.4:82406675-82406694 MS.gene71633:intron 35.0%
! TACTACAGGTTCCCTAATTT+TGG + chr1.4:82408029-82408048 None:intergenic 35.0%
! TCAAGAGTATGCCAAATCTA+TGG - chr1.4:82406413-82406432 MS.gene71633:intron 35.0%
! TCATGAACTTTTACCAGCTT+TGG - chr1.4:82406653-82406672 MS.gene71633:intron 35.0%
!! ATTTGTTTTTCCCAAGGTGA+AGG + chr1.4:82407707-82407726 None:intergenic 35.0%
!! CAGCTTTGGCATATCATTTT+CGG - chr1.4:82406667-82406686 MS.gene71633:intron 35.0%
!! GCTTACGTGTTAAGTTGTTT+TGG - chr1.4:82408354-82408373 MS.gene71633:intron 35.0%
!! GTTGACATTTGTTTTTCCCA+AGG + chr1.4:82407713-82407732 None:intergenic 35.0%
!! TTCCCTAATTTTGGCATCTT+TGG + chr1.4:82408020-82408039 None:intergenic 35.0%
!!! CTTACTTGGTTTTGAAGCAA+GGG + chr1.4:82408281-82408300 None:intergenic 35.0%
!!! TCTGCTGTTTTTAATGGAAG+TGG + chr1.4:82406445-82406464 None:intergenic 35.0%
!!! TCTTACTTGGTTTTGAAGCA+AGG + chr1.4:82408282-82408301 None:intergenic 35.0%
!!! TGATCGTCTGCTGTTTTTAA+TGG + chr1.4:82406451-82406470 None:intergenic 35.0%
AACAGTTTGACATGAGATGG+TGG - chr1.4:82407399-82407418 MS.gene71633:CDS 40.0%
AAGACATCTCCCATGTATGT+CGG + chr1.4:82407500-82407519 None:intergenic 40.0%
ACAGTTTGACATGAGATGGT+GGG - chr1.4:82407400-82407419 MS.gene71633:CDS 40.0%
ACATAGTAACCATCATGAGG+TGG + chr1.4:82408854-82408873 None:intergenic 40.0%
ACGAGTTATCAGTTTGACAG+AGG - chr1.4:82406828-82406847 MS.gene71633:CDS 40.0%
ACGTGAAGAAAAACCCAGAT+GGG - chr1.4:82406595-82406614 MS.gene71633:intron 40.0%
ACTCCAACAAAAGGAGCAAA+AGG + chr1.4:82407074-82407093 None:intergenic 40.0%
AGAAAAACCCAGATGGGAAA+TGG - chr1.4:82406601-82406620 MS.gene71633:intron 40.0%
AGGAAAAGAAAGGTCAGTTC+TGG - chr1.4:82408316-82408335 MS.gene71633:CDS 40.0%
AGTATTGCGTTGAGTGAAGA+AGG - chr1.4:82408106-82408125 MS.gene71633:CDS 40.0%
AGTGGTGAATCCCATAGATT+TGG + chr1.4:82406427-82406446 None:intergenic 40.0%
CACATCCAAGCAATATAGGT+TGG + chr1.4:82407109-82407128 None:intergenic 40.0%
CACTTAAATCTCCTTCACCT+TGG - chr1.4:82407693-82407712 MS.gene71633:CDS 40.0%
CATGATTAGGACAACTTACC+AGG + chr1.4:82408898-82408917 None:intergenic 40.0%
CATGTCATGAACAAACCTGA+AGG + chr1.4:82409249-82409268 None:intergenic 40.0%
CCTCAGTAACCAAACAAAAG+TGG + chr1.4:82407156-82407175 None:intergenic 40.0%
CGAAAATGATATGCCAAAGC+TGG + chr1.4:82406669-82406688 None:intergenic 40.0%
CGAGTTATCAGTTTGACAGA+GGG - chr1.4:82406829-82406848 MS.gene71633:CDS 40.0%
CTGAAGGCTATATCCAAAAG+TGG + chr1.4:82409233-82409252 None:intergenic 40.0%
CTGTAGTAGATAGGACACAA+AGG - chr1.4:82408041-82408060 MS.gene71633:CDS 40.0%
CTTTGTGTCCTATCTACTAC+AGG + chr1.4:82408043-82408062 None:intergenic 40.0%
GAAAAGCAATGACGTACACT+TGG + chr1.4:82407269-82407288 None:intergenic 40.0%
GAAATGTCTAGACAATCTGG+TGG - chr1.4:82406774-82406793 MS.gene71633:intron 40.0%
GGACACAAAGGATACAAACT+AGG - chr1.4:82408053-82408072 MS.gene71633:CDS 40.0%
GGACCAAAGATGCCAAAATT+AGG - chr1.4:82408014-82408033 MS.gene71633:CDS 40.0%
GTAAGAAGAGGAACACAACT+AGG - chr1.4:82408296-82408315 MS.gene71633:CDS 40.0%
GTAGGGTCCATTGAATTCTA+TGG - chr1.4:82409038-82409057 MS.gene71633:CDS 40.0%
GTGTTCCAAAATACCCATCA+TGG + chr1.4:82409071-82409090 None:intergenic 40.0%
GTTAATCAACACAGCATGCA+AGG - chr1.4:82408872-82408891 MS.gene71633:CDS 40.0%
GTTGTGTTCCTCTTCTTACT+TGG + chr1.4:82408295-82408314 None:intergenic 40.0%
TCAAATTCAATGCCGTTAGG+TGG + chr1.4:82406366-82406385 None:intergenic 40.0%
TCAGGCTCATCCTACAATTA+AGG + chr1.4:82409362-82409381 None:intergenic 40.0%
TGATCAAGACAAAGCCTTGA+AGG - chr1.4:82407223-82407242 MS.gene71633:CDS 40.0%
TGATGGTTCACTCCAACAAA+AGG + chr1.4:82407083-82407102 None:intergenic 40.0%
TTAGGGAACCTGTAGTAGAT+AGG - chr1.4:82408032-82408051 MS.gene71633:CDS 40.0%
TTCACTCATTGTACGAAGAC+CGG - chr1.4:82407462-82407481 MS.gene71633:CDS 40.0%
TTGTTTGGTTACTGAGGACA+TGG - chr1.4:82407159-82407178 MS.gene71633:intron 40.0%
! AAACACAAACCATTTGCTGC+AGG + chr1.4:82408474-82408493 None:intergenic 40.0%
! AAAGGTCAGTTCTGGTAATG+TGG - chr1.4:82408324-82408343 MS.gene71633:intron 40.0%
! AACACAAACCATTTGCTGCA+GGG + chr1.4:82408473-82408492 None:intergenic 40.0%
! ACAACAAAGCATTCATGGGA+TGG - chr1.4:82409089-82409108 MS.gene71633:CDS 40.0%
! CACTTTTGGATATAGCCTTC+AGG - chr1.4:82409231-82409250 MS.gene71633:CDS 40.0%
! CGAGAAGGATGAAGAGTTTT+TGG - chr1.4:82407850-82407869 MS.gene71633:CDS 40.0%
! GGAACACAACAAAGCATTCA+TGG - chr1.4:82409084-82409103 MS.gene71633:CDS 40.0%
! TTGCCTTTTGCTCCTTTTGT+TGG - chr1.4:82407068-82407087 MS.gene71633:intron 40.0%
!! AACTATGGGTTGGTTCATGT+AGG - chr1.4:82409180-82409199 MS.gene71633:CDS 40.0%
!! ACTATGGGTTGGTTCATGTA+GGG - chr1.4:82409181-82409200 MS.gene71633:CDS 40.0%
!! AGACTAAGCCCACTTTTGTT+TGG - chr1.4:82407144-82407163 MS.gene71633:intron 40.0%
!! ATGGGAGATGTCTTTTTAGC+TGG - chr1.4:82407506-82407525 MS.gene71633:CDS 40.0%
!! CAAGTTTGCTAACTATGGGT+TGG - chr1.4:82409170-82409189 MS.gene71633:CDS 40.0%
!! CCACTTTTGTTTGGTTACTG+AGG - chr1.4:82407153-82407172 MS.gene71633:intron 40.0%
!! TGTCAATGAAGAGCATCACA+CGG - chr1.4:82406275-82406294 MS.gene71633:CDS 40.0%
AACGACAATGGTGCAGAAAC+TGG - chr1.4:82408211-82408230 MS.gene71633:CDS 45.0%
AAGAAAGCTATGCCCCTCTT+AGG + chr1.4:82406321-82406340 None:intergenic 45.0%
AGAAAGCTATGCCCCTCTTA+GGG + chr1.4:82406320-82406339 None:intergenic 45.0%
ATACCTAGAGCACGCGAATT+TGG + chr1.4:82406499-82406518 None:intergenic 45.0%
CACGTGAAGAAAAACCCAGA+TGG - chr1.4:82406594-82406613 MS.gene71633:intron 45.0%
CACTCAATGCATGTCTGCAA+AGG + chr1.4:82407940-82407959 None:intergenic 45.0%
CACTCCCAACCTATATTGCT+TGG - chr1.4:82407101-82407120 MS.gene71633:intron 45.0%
CCAAAATACCCATCATGGCT+CGG + chr1.4:82409066-82409085 None:intergenic 45.0%
CTACACCCAACACTTCAACT+TGG + chr1.4:82407766-82407785 None:intergenic 45.0%
CTACTGCTTGCATTGCATCA+AGG - chr1.4:82408586-82408605 MS.gene71633:intron 45.0%
CTCGGAGCCATAGAATTCAA+TGG + chr1.4:82409048-82409067 None:intergenic 45.0%
CTGGTGGCTGTCAAAACATT+GGG - chr1.4:82406790-82406809 MS.gene71633:CDS 45.0%
CTTGATGCAATGCAAGCAGT+AGG + chr1.4:82408588-82408607 None:intergenic 45.0%
GAACTGTTGCTGTGAAAGTG+TGG + chr1.4:82409386-82409405 None:intergenic 45.0%
GGGGCATAGCTTTCTTTGAA+GGG - chr1.4:82406325-82406344 MS.gene71633:intron 45.0%
GGTGAAGGAGATTTAAGTGC+AGG + chr1.4:82407692-82407711 None:intergenic 45.0%
GTACGAAGACCGGAAAAAGT+GGG - chr1.4:82407472-82407491 MS.gene71633:CDS 45.0%
GTCAAACTGTTCATCTGTCC+AGG + chr1.4:82407390-82407409 None:intergenic 45.0%
GTGTAGCTGGATGTCAATCT+AGG - chr1.4:82407780-82407799 MS.gene71633:CDS 45.0%
GTTACATGAAGCGCATGCTA+TGG + chr1.4:82408624-82408643 None:intergenic 45.0%
TAACACCTGAGTCTGATGGT+AGG - chr1.4:82406523-82406542 MS.gene71633:intron 45.0%
TCAAGATACCCTGCAGCAAA+TGG - chr1.4:82408462-82408481 MS.gene71633:intron 45.0%
TCGGATGCAATTGCCCTTAA+TGG - chr1.4:82408676-82408695 MS.gene71633:intron 45.0%
TCTGGTGGCTGTCAAAACAT+TGG - chr1.4:82406789-82406808 MS.gene71633:CDS 45.0%
TGTACGAAGACCGGAAAAAG+TGG - chr1.4:82407471-82407490 MS.gene71633:CDS 45.0%
TTAATGGAGCCACCTCATGA+TGG - chr1.4:82408842-82408861 MS.gene71633:CDS 45.0%
TTGAGTGTTCTCCAACGTTG+CGG - chr1.4:82407953-82407972 MS.gene71633:CDS 45.0%
! AAATTCGCGTGCTCTAGGTA+TGG - chr1.4:82406498-82406517 MS.gene71633:intron 45.0%
! ACGGATTTTGAGCCACCTAA+CGG - chr1.4:82406351-82406370 MS.gene71633:intron 45.0%
! AGCTTTCTTTGAAGGGGACA+CGG - chr1.4:82406332-82406351 MS.gene71633:intron 45.0%
! AGGGGCATAGCTTTCTTTGA+AGG - chr1.4:82406324-82406343 MS.gene71633:intron 45.0%
! GCAATATAGGTTGGGAGTGA+TGG + chr1.4:82407100-82407119 None:intergenic 45.0%
! GGGCATAGCTTTCTTTGAAG+GGG - chr1.4:82406326-82406345 MS.gene71633:intron 45.0%
! TATCGTCCGACAACCACTTT+TGG - chr1.4:82409217-82409236 MS.gene71633:CDS 45.0%
! TGGATGTCAATCTAGGACAG+AGG - chr1.4:82407787-82407806 MS.gene71633:CDS 45.0%
! TGTCAATCTAGGACAGAGGT+TGG - chr1.4:82407791-82407810 MS.gene71633:CDS 45.0%
! TTGCTGTGAAAGTGTGGATC+AGG + chr1.4:82409380-82409399 None:intergenic 45.0%
!! AAAGTGGGTACCGACATACA+TGG - chr1.4:82407487-82407506 MS.gene71633:CDS 45.0%
!! AAGTGGGTACCGACATACAT+GGG - chr1.4:82407488-82407507 MS.gene71633:CDS 45.0%
!! CAGTATCTGGCTTTGGATGA+GGG - chr1.4:82406852-82406871 MS.gene71633:intron 45.0%
!! GTCAATGAAGAGCATCACAC+GGG - chr1.4:82406276-82406295 MS.gene71633:CDS 45.0%
!!! ATGTCGGTACCCACTTTTTC+CGG + chr1.4:82407484-82407503 None:intergenic 45.0%
ACTGGTTGCTTCCCCTAAGA+GGG - chr1.4:82406305-82406324 MS.gene71633:intron 50.0%
AGGACATGGCTTAGAGCGAT+GGG - chr1.4:82407173-82407192 MS.gene71633:intron 50.0%
ATGCCAAATTCGCGTGCTCT+AGG - chr1.4:82406493-82406512 MS.gene71633:intron 50.0%
CACTTGAACAACCGCAACGT+TGG + chr1.4:82407967-82407986 None:intergenic 50.0%
GAAAGCTATGCCCCTCTTAG+GGG + chr1.4:82406319-82406338 None:intergenic 50.0%
GGAGTAACACCTGAGTCTGA+TGG - chr1.4:82406519-82406538 MS.gene71633:intron 50.0%
GGTACTCAGCAGAATGTGCA+AGG - chr1.4:82408706-82408725 MS.gene71633:CDS 50.0%
GGTGCAGAAACTGGCAACAA+TGG - chr1.4:82408220-82408239 MS.gene71633:CDS 50.0%
GTTCATGTAGGGAGGGCAAT+TGG - chr1.4:82409192-82409211 MS.gene71633:CDS 50.0%
GTTTGACAGAGGGCAGTATC+TGG - chr1.4:82406839-82406858 MS.gene71633:intron 50.0%
TCAGCAGAATGTGCAAGGAC+TGG - chr1.4:82408711-82408730 MS.gene71633:CDS 50.0%
TTCACACTGTCGGAACGTTG+AGG + chr1.4:82407542-82407561 None:intergenic 50.0%
! TCTAGGACAGAGGTTGGAGA+TGG - chr1.4:82407797-82407816 MS.gene71633:CDS 50.0%
! TTGGAACGAGTTGAGCTCAG+AGG - chr1.4:82407877-82407896 MS.gene71633:CDS 50.0%
!! AATGAAGAGCATCACACGGG+TGG - chr1.4:82406279-82406298 MS.gene71633:CDS 50.0%
!! ATGGGTTGGTTCATGTAGGG+AGG - chr1.4:82409184-82409203 MS.gene71633:CDS 50.0%
!! GCAGTATCTGGCTTTGGATG+AGG - chr1.4:82406851-82406870 MS.gene71633:intron 50.0%
!! GTTGAAGTGTTGGGTGTAGC+TGG - chr1.4:82407767-82407786 MS.gene71633:CDS 50.0%
!! TCTTGAGCGTCGACAAGTAC+AGG + chr1.4:82408448-82408467 None:intergenic 50.0%
!! TGGCTTTGGATGAGGGAGAT+GGG - chr1.4:82406859-82406878 MS.gene71633:intron 50.0%
!! TGGGTTGGTTCATGTAGGGA+GGG - chr1.4:82409185-82409204 MS.gene71633:CDS 50.0%
!!! CCGAGCCATGATGGGTATTT+TGG - chr1.4:82409063-82409082 MS.gene71633:CDS 50.0%
ACATGGCTTAGAGCGATGGG+AGG - chr1.4:82407176-82407195 MS.gene71633:intron 55.0%
ACTGTCGGAACGTTGAGGTG+TGG + chr1.4:82407537-82407556 None:intergenic 55.0%
CATGGCTTAGAGCGATGGGA+GGG - chr1.4:82407177-82407196 MS.gene71633:intron 55.0%
CTATGGCTCCGAGCCATGAT+GGG - chr1.4:82409055-82409074 MS.gene71633:CDS 55.0%
CTGCTCCTACCATCAGACTC+AGG + chr1.4:82406531-82406550 None:intergenic 55.0%
CTGGTTGCTTCCCCTAAGAG+GGG - chr1.4:82406306-82406325 MS.gene71633:intron 55.0%
GAGGACATGGCTTAGAGCGA+TGG - chr1.4:82407172-82407191 MS.gene71633:intron 55.0%
GGGAGATGGGCAAGTTATGC+TGG - chr1.4:82406872-82406891 MS.gene71633:intron 55.0%
TCAACACAGCATGCAAGGCC+TGG - chr1.4:82408877-82408896 MS.gene71633:CDS 55.0%
TCTATGGCTCCGAGCCATGA+TGG - chr1.4:82409054-82409073 MS.gene71633:CDS 55.0%
! CACTGGTTGCTTCCCCTAAG+AGG - chr1.4:82406304-82406323 MS.gene71633:intron 55.0%
! CAGAGGGCAGTATCTGGCTT+TGG - chr1.4:82406845-82406864 MS.gene71633:intron 55.0%
!! CTGGCTTTGGATGAGGGAGA+TGG - chr1.4:82406858-82406877 MS.gene71633:intron 55.0%
GCATCACACGGGTGGTGCAC+TGG - chr1.4:82406287-82406306 MS.gene71633:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 82406264 82409418 82406264 ID=MS.gene71633
chr1.4 mRNA 82406264 82409418 82406264 ID=MS.gene71633.t1;Parent=MS.gene71633
chr1.4 exon 82408699 82409418 82408699 ID=MS.gene71633.t1.exon1;Parent=MS.gene71633.t1
chr1.4 CDS 82408699 82409418 82408699 ID=cds.MS.gene71633.t1;Parent=MS.gene71633.t1
chr1.4 exon 82407355 82408338 82407355 ID=MS.gene71633.t1.exon2;Parent=MS.gene71633.t1
chr1.4 CDS 82407355 82408338 82407355 ID=cds.MS.gene71633.t1;Parent=MS.gene71633.t1
chr1.4 exon 82407199 82407255 82407199 ID=MS.gene71633.t1.exon3;Parent=MS.gene71633.t1
chr1.4 CDS 82407199 82407255 82407199 ID=cds.MS.gene71633.t1;Parent=MS.gene71633.t1
chr1.4 exon 82406950 82407018 82406950 ID=MS.gene71633.t1.exon4;Parent=MS.gene71633.t1
chr1.4 CDS 82406950 82407018 82406950 ID=cds.MS.gene71633.t1;Parent=MS.gene71633.t1
chr1.4 exon 82406784 82406855 82406784 ID=MS.gene71633.t1.exon5;Parent=MS.gene71633.t1
chr1.4 CDS 82406784 82406855 82406784 ID=cds.MS.gene71633.t1;Parent=MS.gene71633.t1
chr1.4 exon 82406430 82406480 82406430 ID=MS.gene71633.t1.exon6;Parent=MS.gene71633.t1
chr1.4 CDS 82406430 82406480 82406430 ID=cds.MS.gene71633.t1;Parent=MS.gene71633.t1
chr1.4 exon 82406264 82406311 82406264 ID=MS.gene71633.t1.exon7;Parent=MS.gene71633.t1
chr1.4 CDS 82406264 82406311 82406264 ID=cds.MS.gene71633.t1;Parent=MS.gene71633.t1
Gene Sequence

>MS.gene71633

ATGGGTGACTCTGTCAATGAAGAGCATCACACGGGTGGTGCACTGGTTGCTTCCCCTAAGAGGGGCATAGCTTTCTTTGAAGGGGACACGGATTTTGAGCCACCTAACGGCATTGAATTTGAAACTCATGAAGCAGCATATTCATTTTATCAAGAGTATGCCAAATCTATGGGATTCACCACTTCCATTAAAAACAGCAGACGATCAAAGAAGACCAAAGAATTTATTGATGCCAAATTCGCGTGCTCTAGGTATGGAGTAACACCTGAGTCTGATGGTAGGAGCAGTCGAAGATCGAGTGTAAAGAAGACAGATTGCAAAGCTTGCATGCACGTGAAGAAAAACCCAGATGGGAAATGGAACATTTTTGAATTTATAAAGGATCATAATCATGAACTTTTACCAGCTTTGGCATATCATTTTCGGATTCATGGAAACGTGAAGTTAGCTGACAAGAATAATATTGATATCTTGCATGCTGTTAGTGAACGTACCCGAAAGATGTATGTTGAAATGTCTAGACAATCTGGTGGCTGTCAAAACATTGGGTCAATCATGGGTGATACGAGTTATCAGTTTGACAGAGGGCAGTATCTGGCTTTGGATGAGGGAGATGGGCAAGTTATGCTGGAGTATTTCAAGCATATTCAAAAGGAGGATCCCAACTTCTTCTATTCTATAGATTTAAACGAGGAACAACGTTTAAGAAATATATTTTGGGTTGATTCAAAAAGCATCGATGATTACCTCAGCTTTAATGATGCAGTTTCATTTGATACTTCCTACATAAAAAGCAACGATAAGTTGCCTTTTGCTCCTTTTGTTGGAGTGAACCATCACTCCCAACCTATATTGCTTGGATGTGCATTGGTTGCTGATGAGACTAAGCCCACTTTTGTTTGGTTACTGAGGACATGGCTTAGAGCGATGGGAGGGCAAGCTCCCAAAGTTATAGTCACTGATCAAGACAAAGCCTTGAAGGAAGCAATTGAAGAAGTCTTCCCAAGTGTACGTCATTGCTTTTCTCTTTGTCACATATTGGAAAAGATACCTGAAAATCTGTCTTTTGTGATAAAACAGTACAAGAACTTCTTGCCAAAGTTTAAGAATTGCATTTTTAAGTCCTGGACAGATGAACAGTTTGACATGAGATGGTGGGAAATAGTGACTATATTTGAACTACATGATGATGTTTGGTTTCACTCATTGTACGAAGACCGGAAAAAGTGGGTACCGACATACATGGGAGATGTCTTTTTAGCTGGAATGTCCACACCTCAACGTTCCGACAGTGTGAACTCTTTCTTTGACAAATACATTCATAAGAAAATCACCCTTAAAGAGTTTGTGAAACAGTATAGGTTAATTCTTCTGAATAGGTATGATGAGGAAGAAATCGCAGATTTTGATACATTGCACAAACAACCTGCACTTAAATCTCCTTCACCTTGGGAAAAACAAATGTCAACAATTTATACACATGCAATATTTAAGAAATTCCAAGTTGAAGTGTTGGGTGTAGCTGGATGTCAATCTAGGACAGAGGTTGGAGATGGAACTGTTGTGAGATATATAGTTCAAGATTACGAGAAGGATGAAGAGTTTTTGGTAACTTGGAACGAGTTGAGCTCAGAGGTCTCTTGCTTCTGTCGATTGTTCGAATATAAAGGTTTCCTTTGCAGACATGCATTGAGTGTTCTCCAACGTTGCGGTTGTTCAAGTGTTCCATCTCATTATATCATGAAGAGATGGACCAAAGATGCCAAAATTAGGGAACCTGTAGTAGATAGGACACAAAGGATACAAACTAGGCCTCAACGTTATAATGATGTGTGCAAACGAAGTATTGCGTTGAGTGAAGAAGGATCATCATCTGAAGAGAGTTATAATGTTGCTTTGCGCGCGCTTGTTGATGCTTTGAAGAACTGTGTACTTGTAAATAACTCTAACGACAATGGTGCAGAAACTGGCAACAATGGTTACAGTCTTCGCGAAGCAGAAGAATATCAGAGTACCCTTGCTTCAAAACCAAGTAAGAAGAGGAACACAACTAGGAAAAGAAAGGTCAGTTCTGGTAATGTGGTTTTTGTGCTTACGTGTTAAGTTGTTTTGGCTTTCTAGCATTTAAAAGATCAGGAAATTGATACGAGAATCACTTTATAGGTGCAACAAGAGCAGAGTCCTGTACTTGTCGACGCTCAAGATACCCTGCAGCAAATGGTTTGTGTTTCCTCTTTTTTCATTCATGATCTGTTTTAGATTATACAAATAAGCATGGTACATCCATCCTCTTGAGAATTGCATTTCTATTTTCATGAATTCCTACTGCTTGCATTGCATCAAGGCTGAAAAGAGATCCATAGCATGCGCTTCATGTAACATGTGCTTTCGTTCTTTTAGGATAATCTTAGCTCGGATGCAATTGCCCTTAATGGATATTATGGTACTCAGCAGAATGTGCAAGGACTGGTAAATTTACATGTTAACCATCAAATGATTACAATGTTCTGTATTCTAAGAACTAAAAGTAATGCTTAATAGATTGTAATTTTGGTTTCTGTAGCATGTACAATTGAACTTAATGGAGCCACCTCATGATGGTTACTATGTTAATCAACACAGCATGCAAGGCCTGGTAAGTTGTCCTAATCATGAATATAATCTTCTGATACGATTATCTTATATAATTAATTATTAATTATAAAGGAATAAGATTATTTATTAATTGTATTTCAAAAAAAAAAATTATTTATTAAATTGATATTCATATCGTTGTAGGGTCCATTGAATTCTATGGCTCCGAGCCATGATGGGTATTTTGGAACACAACAAAGCATTCATGGGATGGTAGAGTACTTTCCTATGAAATTATTGGGAAATTATATTAATTAACATTAATTATGATACAAGTTTGCTAACTATGGGTTGGTTCATGTAGGGAGGGCAATTGGAGTATCGTCCGACAACCACTTTTGGATATAGCCTTCAGGTTTGTTCATGACATGTACCGTATACTTAGTTACTTATTAGCATTTGAACTTGGAGATTTGAATAGAATAATTTGTGCTTGGTTTTCACCGTCTGTTTGCACATAACCTTAATTGTAGGATGAGCCTGATCCACACTTTCACAGCAACAGTTCAAGAAATACTTAG
Protein sequence

>MS.gene71633.t1

MGDSVNEEHHTGGALVASPKRGIAFFEGDTDFEPPNGIEFETHEAAYSFYQEYAKSMGFTTSIKNSRRSKKTKEFIDAKFACSRYGVTPESDGRSSRRSSVKKTDCKACMHVKKNPDGKWNIFEFIKDHNHELLPALAYHFRIHGNVKLADKNNIDILHAVSERTRKMYVEMSRQSGGCQNIGSIMGDTSYQFDRGQYLALDEGDGQVMLEYFKHIQKEDPNFFYSIDLNEEQRLRNIFWYKNFLPKFKNCIFKSWTDEQFDMRWWEIVTIFELHDDVWFHSLYEDRKKWVPTYMGDVFLAGMSTPQRSDSVNSFFDKYIHKKITLKEFVKQYRLILLNRYDEEEIADFDTLHKQPALKSPSPWEKQMSTIYTHAIFKKFQVEVLGVAGCQSRTEVGDGTVVRYIVQDYEKDEEFLVTWNELSSEVSCFCRLFEYKGFLCRHALSVLQRCGCSSVPSHYIMKRWTKDAKIREPVVDRTQRIQTRPQRYNDVCKRSIALSEEGSSSEESYNVALRALVDALKNCVLVNNSNDNGAETGNNGYSLREAEEYQSTLASKPSKKRNTTRKRKVQQEQSPVLVDAQDTLQQMDNLSSDAIALNGYYGTQQNVQGLHVQLNLMEPPHDGYYVNQHSMQGLGGQLEYRPTTTFGYSLQDEPDPHFHSNSSRNT