Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71661.t1 | RHN82242.1 | 97.8 | 540 | 9 | 1 | 2 | 538 | 96 | 635 | 6.60E-289 | 1002.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71661.t1 | Q921I2 | 37.8 | 418 | 229 | 12 | 2 | 404 | 90 | 491 | 3.0e-72 | 274.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71661.t1 | A0A396JXG1 | 97.8 | 540 | 9 | 1 | 2 | 538 | 96 | 635 | 4.8e-289 | 1002.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055358 | MS.gene71661 | 0.802345 | 5.86E-49 | -1.69E-46 |
| MS.gene060167 | MS.gene71661 | 0.802268 | 6.08E-49 | -1.69E-46 |
| MS.gene060312 | MS.gene71661 | 0.848964 | 4.38E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71661.t1 | MTR_1g107265 | 92.147 | 573 | 9 | 3 | 2 | 538 | 96 | 668 | 0.0 | 1080 |
| MS.gene71661.t1 | MTR_1g107265 | 28.070 | 171 | 106 | 6 | 82 | 249 | 65 | 221 | 5.72e-13 | 72.0 |
| MS.gene71661.t1 | MTR_1g107265 | 92.147 | 573 | 9 | 3 | 2 | 538 | 96 | 668 | 0.0 | 1077 |
| MS.gene71661.t1 | MTR_1g107265 | 28.070 | 171 | 106 | 6 | 82 | 249 | 65 | 221 | 6.45e-13 | 72.0 |
| MS.gene71661.t1 | MTR_2g069030 | 31.102 | 254 | 148 | 10 | 9 | 259 | 50 | 279 | 2.16e-22 | 98.6 |
| MS.gene71661.t1 | MTR_2g069030 | 29.911 | 224 | 129 | 11 | 8 | 220 | 100 | 306 | 3.67e-15 | 77.0 |
| MS.gene71661.t1 | MTR_1g084120 | 26.087 | 253 | 155 | 8 | 6 | 255 | 52 | 275 | 2.69e-19 | 92.0 |
| MS.gene71661.t1 | MTR_4g109680 | 26.104 | 249 | 146 | 11 | 9 | 249 | 55 | 273 | 2.36e-16 | 82.4 |
| MS.gene71661.t1 | MTR_4g109680 | 33.333 | 126 | 72 | 5 | 6 | 128 | 158 | 274 | 1.87e-14 | 76.6 |
| MS.gene71661.t1 | MTR_4g109680 | 25.714 | 245 | 155 | 8 | 11 | 252 | 5 | 225 | 3.62e-14 | 75.9 |
| MS.gene71661.t1 | MTR_2g019860 | 25.073 | 343 | 196 | 14 | 8 | 314 | 115 | 432 | 2.96e-16 | 82.4 |
| MS.gene71661.t1 | MTR_4g119100 | 28.216 | 241 | 141 | 10 | 3 | 232 | 117 | 336 | 3.08e-16 | 82.4 |
| MS.gene71661.t1 | MTR_4g119100 | 28.216 | 241 | 141 | 10 | 3 | 232 | 118 | 337 | 3.49e-16 | 82.4 |
| MS.gene71661.t1 | MTR_4g119100 | 28.216 | 241 | 141 | 10 | 3 | 232 | 118 | 337 | 4.45e-16 | 82.0 |
| MS.gene71661.t1 | MTR_4g132120 | 26.977 | 215 | 131 | 8 | 8 | 221 | 316 | 505 | 3.56e-15 | 79.3 |
| MS.gene71661.t1 | MTR_7g113200 | 25.391 | 256 | 141 | 11 | 6 | 249 | 165 | 382 | 5.52e-13 | 72.0 |
| MS.gene71661.t1 | MTR_7g113200 | 25.792 | 221 | 135 | 9 | 8 | 220 | 213 | 412 | 1.31e-11 | 67.8 |
| MS.gene71661.t1 | MTR_1g090740 | 25.703 | 249 | 133 | 10 | 19 | 249 | 15 | 229 | 6.96e-13 | 71.2 |
| MS.gene71661.t1 | MTR_7g113200 | 25.391 | 256 | 141 | 11 | 6 | 249 | 165 | 382 | 3.74e-12 | 68.9 |
| MS.gene71661.t1 | MTR_7g113200 | 25.792 | 221 | 135 | 9 | 8 | 220 | 213 | 412 | 5.38e-11 | 65.5 |
| MS.gene71661.t1 | MTR_1g492850 | 25.877 | 228 | 132 | 8 | 8 | 220 | 47 | 252 | 3.92e-12 | 68.9 |
| MS.gene71661.t1 | MTR_1g492850 | 25.877 | 228 | 132 | 8 | 8 | 220 | 47 | 252 | 4.71e-12 | 68.9 |
| MS.gene71661.t1 | MTR_1g089370 | 28.175 | 252 | 152 | 14 | 5 | 247 | 72 | 303 | 1.23e-11 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71661.t1 | AT5G50310 | 72.110 | 545 | 138 | 7 | 2 | 538 | 96 | 634 | 0.0 | 741 |
| MS.gene71661.t1 | AT3G07720 | 29.918 | 244 | 144 | 10 | 9 | 249 | 52 | 271 | 1.76e-16 | 81.3 |
| MS.gene71661.t1 | AT4G04670 | 25.728 | 206 | 124 | 6 | 20 | 221 | 314 | 494 | 7.04e-16 | 81.6 |
| MS.gene71661.t1 | AT1G18610 | 32.283 | 127 | 76 | 4 | 6 | 130 | 158 | 276 | 7.89e-14 | 74.7 |
| MS.gene71661.t1 | AT1G18610 | 32.283 | 127 | 76 | 4 | 6 | 130 | 158 | 276 | 7.89e-14 | 74.7 |
| MS.gene71661.t1 | AT3G05420 | 27.313 | 227 | 131 | 9 | 7 | 220 | 210 | 415 | 8.84e-14 | 74.7 |
| MS.gene71661.t1 | AT3G05420 | 27.313 | 227 | 131 | 9 | 7 | 220 | 209 | 414 | 9.30e-14 | 74.7 |
| MS.gene71661.t1 | AT1G74150 | 24.597 | 248 | 151 | 10 | 9 | 249 | 52 | 270 | 1.44e-13 | 73.9 |
| MS.gene71661.t1 | AT1G74150 | 32.283 | 127 | 76 | 4 | 6 | 130 | 155 | 273 | 1.13e-12 | 70.9 |
| MS.gene71661.t1 | AT1G74150 | 26.108 | 203 | 127 | 8 | 23 | 220 | 14 | 198 | 6.42e-12 | 68.6 |
| MS.gene71661.t1 | AT1G74150 | 24.597 | 248 | 151 | 10 | 9 | 249 | 52 | 270 | 1.49e-13 | 73.6 |
| MS.gene71661.t1 | AT1G74150 | 32.283 | 127 | 76 | 4 | 6 | 130 | 155 | 273 | 1.14e-12 | 70.9 |
| MS.gene71661.t1 | AT1G74150 | 26.108 | 203 | 127 | 8 | 23 | 220 | 14 | 198 | 6.52e-12 | 68.6 |
| MS.gene71661.t1 | AT1G74150 | 24.597 | 248 | 151 | 10 | 9 | 249 | 52 | 270 | 1.62e-13 | 73.6 |
| MS.gene71661.t1 | AT1G74150 | 32.283 | 127 | 76 | 4 | 6 | 130 | 155 | 273 | 1.14e-12 | 70.9 |
| MS.gene71661.t1 | AT1G74150 | 26.108 | 203 | 127 | 8 | 23 | 220 | 14 | 198 | 6.85e-12 | 68.6 |
| MS.gene71661.t1 | AT1G74150 | 25.000 | 228 | 136 | 9 | 28 | 249 | 56 | 254 | 3.18e-13 | 72.8 |
| MS.gene71661.t1 | AT1G74150 | 32.283 | 127 | 76 | 4 | 6 | 130 | 139 | 257 | 1.03e-12 | 70.9 |
| MS.gene71661.t1 | AT2G33070 | 23.443 | 273 | 182 | 7 | 9 | 278 | 198 | 446 | 4.49e-13 | 72.0 |
| MS.gene71661.t1 | AT2G33070 | 23.443 | 273 | 182 | 7 | 9 | 278 | 196 | 444 | 5.52e-13 | 71.6 |
| MS.gene71661.t1 | AT2G33070 | 23.443 | 273 | 182 | 7 | 9 | 278 | 196 | 444 | 5.52e-13 | 71.6 |
| MS.gene71661.t1 | AT5G27630 | 26.432 | 227 | 133 | 8 | 7 | 220 | 210 | 415 | 8.68e-13 | 71.6 |
| MS.gene71661.t1 | AT5G27630 | 26.432 | 227 | 133 | 8 | 7 | 220 | 210 | 415 | 9.66e-13 | 71.2 |
| MS.gene71661.t1 | AT5G27630 | 26.432 | 227 | 133 | 8 | 7 | 220 | 210 | 415 | 1.06e-12 | 71.2 |
| MS.gene71661.t1 | AT5G27630 | 26.432 | 227 | 133 | 8 | 7 | 220 | 210 | 415 | 1.50e-12 | 70.5 |
| MS.gene71661.t1 | AT1G54040 | 26.000 | 250 | 165 | 8 | 8 | 249 | 45 | 282 | 2.98e-12 | 68.6 |
| MS.gene71661.t1 | AT1G54040 | 26.000 | 250 | 165 | 8 | 8 | 249 | 51 | 288 | 3.38e-12 | 68.6 |
| MS.gene71661.t1 | AT3G16400 | 23.443 | 273 | 182 | 7 | 9 | 278 | 195 | 443 | 2.64e-11 | 66.2 |
| MS.gene71661.t1 | AT3G16400 | 23.443 | 273 | 182 | 7 | 9 | 278 | 195 | 443 | 2.64e-11 | 66.2 |
| MS.gene71661.t1 | AT3G16410 | 24.180 | 244 | 158 | 7 | 9 | 249 | 344 | 563 | 3.11e-11 | 66.6 |
| MS.gene71661.t1 | AT5G48180 | 27.049 | 244 | 144 | 11 | 8 | 246 | 49 | 263 | 4.63e-11 | 64.7 |
Find 128 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGACCTTGATCAATTTA+AGG | 0.143374 | 1.4:+82674332 | MS.gene71661:CDS |
| TGGGAACAACTTAATTTAAA+AGG | 0.179060 | 1.4:+82672855 | MS.gene71661:CDS |
| TTGTTCTTGAATGAGCTTTA+TGG | 0.213686 | 1.4:+82675503 | MS.gene71661:CDS |
| TAATCTTTGATGCAATATTC+TGG | 0.265820 | 1.4:-82676024 | None:intergenic |
| AAGAGGAGGGCTCTGCTATT+TGG | 0.302506 | 1.4:+82675100 | MS.gene71661:CDS |
| CAGTGTGCTGAGTGTGTTCA+TGG | 0.316496 | 1.4:-82679648 | None:intergenic |
| TGATGGAACCTCTCTTGATT+TGG | 0.345284 | 1.4:-82672684 | None:intergenic |
| CTGCGGGGAGGAGGACTATT+AGG | 0.349990 | 1.4:-82672510 | None:intergenic |
| CCTGGAGCAATGTGGCCAAC+TGG | 0.354930 | 1.4:+82674528 | MS.gene71661:CDS |
| AAGAGATGAAGCTGAGGATA+TGG | 0.355774 | 1.4:+82675970 | MS.gene71661:CDS |
| ATGCCTCCTGGTCCTCGTGC+AGG | 0.359177 | 1.4:+82675058 | MS.gene71661:CDS |
| TATATGCAGACTTTCGTCTA+TGG | 0.366544 | 1.4:+82672435 | None:intergenic |
| GATTCCTTAGACTTTCCTTC+AGG | 0.366560 | 1.4:-82676076 | None:intergenic |
| CTTAGACTTTCCTTCAGGTT+TGG | 0.384258 | 1.4:-82676071 | None:intergenic |
| CTTGTTCCATTCCCAAGTCT+TGG | 0.391108 | 1.4:-82674875 | None:intergenic |
| GGGTGTCCTGGTCCACGTTC+GGG | 0.392989 | 1.4:+82672876 | MS.gene71661:CDS |
| CAAACCTGAAGGAAAGTCTA+AGG | 0.399665 | 1.4:+82676072 | MS.gene71661:CDS |
| TACAAATTTGTATTGGCAAA+TGG | 0.410540 | 1.4:+82679621 | MS.gene71661:CDS |
| TTGTTCCATTCCCAAGTCTT+GGG | 0.413147 | 1.4:-82674874 | None:intergenic |
| TCATCTCTTGCAGTAGATAG+TGG | 0.413970 | 1.4:-82675956 | None:intergenic |
| AGAGATACACTATATATATA+TGG | 0.419292 | 1.4:+82676271 | MS.gene71661:CDS |
| GGCCACATTGCTCCAGGCTT+AGG | 0.421712 | 1.4:-82674522 | None:intergenic |
| TTAAGCGTACAAATTTGTAT+TGG | 0.421780 | 1.4:+82679614 | MS.gene71661:CDS |
| ATTCCCAAGTCTTGGGATCA+AGG | 0.424658 | 1.4:-82674867 | None:intergenic |
| TTTCCATGTGTGTTCATAAG+AGG | 0.428365 | 1.4:+82675083 | MS.gene71661:CDS |
| AGGGTGTCCTGGTCCACGTT+CGG | 0.432220 | 1.4:+82672875 | MS.gene71661:CDS |
| CCAGTTGGCCACATTGCTCC+AGG | 0.435281 | 1.4:-82674528 | None:intergenic |
| AACAGCTTGATGAGCACTGC+GGG | 0.441731 | 1.4:-82672526 | None:intergenic |
| GAAGGAAAGTCTAAGGAATC+TGG | 0.444652 | 1.4:+82676079 | MS.gene71661:CDS |
| AATCTTTGATGCAATATTCT+GGG | 0.445092 | 1.4:-82676023 | None:intergenic |
| ATCTTGCAGAAACACGATAC+AGG | 0.447457 | 1.4:+82681127 | MS.gene71661:CDS |
| TGCCTCCTGGTCCTCGTGCA+GGG | 0.455126 | 1.4:+82675059 | MS.gene71661:CDS |
| AATTTACCTTAAATTGATCA+AGG | 0.459598 | 1.4:-82674338 | None:intergenic |
| TGATGAGCACTGCGGGGAGG+AGG | 0.462419 | 1.4:-82672519 | None:intergenic |
| GGGAACAACTTAATTTAAAA+GGG | 0.462637 | 1.4:+82672856 | MS.gene71661:CDS |
| TCGATATGATGTTGAGAAGC+TGG | 0.469863 | 1.4:+82672467 | MS.gene71661:CDS |
| CGATGTCCCGAACGTGGACC+AGG | 0.482739 | 1.4:-82672882 | None:intergenic |
| GTGCTCATCAAGCTGTTGCT+TGG | 0.482807 | 1.4:+82672532 | MS.gene71661:CDS |
| ATCTCTTCCAACAGCCATGC+AGG | 0.483354 | 1.4:-82676254 | None:intergenic |
| CCCAGGCATCAGAATCTGAA+TGG | 0.483703 | 1.4:+82677330 | MS.gene71661:intron |
| ACCTTAGCAAACTTGATGAA+TGG | 0.486761 | 1.4:+82676350 | MS.gene71661:CDS |
| CTTAATTTAAAAGGGTGTCC+TGG | 0.493693 | 1.4:+82672864 | MS.gene71661:CDS |
| GCAGGAATTAATGCGTCCAC+AGG | 0.496059 | 1.4:-82676236 | None:intergenic |
| CTGGAGCAATGTGGCCAACT+GGG | 0.504537 | 1.4:+82674529 | MS.gene71661:CDS |
| CAGATTTAACCTTGTAGTGA+TGG | 0.505260 | 1.4:-82672701 | None:intergenic |
| TCCATTCATCAAGTTTGCTA+AGG | 0.506196 | 1.4:-82676351 | None:intergenic |
| GGTCCCTTGATCCCAAGACT+TGG | 0.508965 | 1.4:+82674863 | MS.gene71661:CDS |
| GACAAGAATGCCTCTGAGAA+AGG | 0.512410 | 1.4:+82674820 | MS.gene71661:CDS |
| TCTGCTATTTGGTGGTGTTG+TGG | 0.514556 | 1.4:+82675111 | MS.gene71661:CDS |
| AGGAGGGCTCTGCTATTTGG+TGG | 0.519472 | 1.4:+82675103 | MS.gene71661:CDS |
| TGAATGGAAGTGCATTATAC+CGG | 0.521369 | 1.4:+82676366 | MS.gene71661:CDS |
| AAGAAGAGTGGGATGCCTCC+TGG | 0.521741 | 1.4:+82675046 | MS.gene71661:CDS |
| AGATGCTGTAGCTTTAATCA+AGG | 0.526316 | 1.4:+82679261 | MS.gene71661:CDS |
| GAAAACCCTGCACGAGGACC+AGG | 0.526655 | 1.4:-82675064 | None:intergenic |
| CCATGTGTGTTCATAAGAGG+AGG | 0.533379 | 1.4:+82675086 | MS.gene71661:CDS |
| CTGGTCCACGTTCGGGACAT+CGG | 0.535631 | 1.4:+82672883 | MS.gene71661:CDS |
| GGATAAATCAACAAAAGATA+AGG | 0.537288 | 1.4:+82675780 | MS.gene71661:CDS |
| TTCAGTTAGACACCAATCGC+TGG | 0.538855 | 1.4:+82675528 | MS.gene71661:CDS |
| ATTAATTCCTGCATGGCTGT+TGG | 0.539010 | 1.4:+82676247 | MS.gene71661:CDS |
| GAATTGTTCATTCAGATATG+TGG | 0.539563 | 1.4:+82674842 | MS.gene71661:CDS |
| CAAGAAATAAAGCCTAAGCC+TGG | 0.543576 | 1.4:+82674510 | MS.gene71661:CDS |
| GTACCCTCTGGAGTTAAGAA+AGG | 0.548785 | 1.4:+82675759 | MS.gene71661:CDS |
| CCAGGCATCAGAATCTGAAT+GGG | 0.549368 | 1.4:+82677331 | MS.gene71661:intron |
| CTTGGTAGGTTAAGAAGAGT+GGG | 0.551093 | 1.4:+82675035 | MS.gene71661:intron |
| TCTTGGTAGGTTAAGAAGAG+TGG | 0.551525 | 1.4:+82675034 | MS.gene71661:intron |
| TTTGTGAAATAGGTACCCTC+TGG | 0.553079 | 1.4:+82675747 | MS.gene71661:intron |
| TTCCCAAGTCTTGGGATCAA+GGG | 0.555002 | 1.4:-82674866 | None:intergenic |
| ATATATATATGGTGGCATGA+TGG | 0.561842 | 1.4:+82676282 | MS.gene71661:CDS |
| AAGTGTTTCATTGTCAGCAA+TGG | 0.562261 | 1.4:-82676047 | None:intergenic |
| TATCCTTTCTTAACTCCAGA+GGG | 0.568713 | 1.4:-82675762 | None:intergenic |
| AATCTAAACTTACCAGCGAT+TGG | 0.570438 | 1.4:-82675540 | None:intergenic |
| TGATAGGAACTCCGCAAGGA+TGG | 0.570747 | 1.4:+82681099 | MS.gene71661:intron |
| GTCCCTTGATCCCAAGACTT+GGG | 0.570914 | 1.4:+82674864 | MS.gene71661:CDS |
| CAAGACTTGGGAATGGAACA+AGG | 0.575951 | 1.4:+82674876 | MS.gene71661:CDS |
| AGTGTGCTGAGTGTGTTCAT+GGG | 0.577454 | 1.4:-82679647 | None:intergenic |
| TACTAACGATGTCGACAAGA+AGG | 0.579404 | 1.4:+82675928 | MS.gene71661:CDS |
| GAGCAAATCAGAGCTAGCCT+TGG | 0.579750 | 1.4:+82679328 | MS.gene71661:CDS |
| GGCATCAGAATCTGAATGGG+TGG | 0.581599 | 1.4:+82677334 | MS.gene71661:intron |
| CATTCAAAATTCTTTGCCCG+AGG | 0.582771 | 1.4:+82676114 | MS.gene71661:CDS |
| TTGATCCCAAGACTTGGGAA+TGG | 0.585173 | 1.4:+82674869 | MS.gene71661:CDS |
| TTATCCTTTCTTAACTCCAG+AGG | 0.586418 | 1.4:-82675763 | None:intergenic |
| TCTGAATGCATCAGATCAAG+TGG | 0.588802 | 1.4:+82679237 | MS.gene71661:CDS |
| CATGAACACACTCAGCACAC+TGG | 0.589985 | 1.4:+82679649 | MS.gene71661:CDS |
| TGTAGCTTTAATCAAGGGAG+GGG | 0.595643 | 1.4:+82679267 | MS.gene71661:CDS |
| CTGTAGCTTTAATCAAGGGA+GGG | 0.597224 | 1.4:+82679266 | MS.gene71661:CDS |
| TCTTGCAGAAACACGATACA+GGG | 0.600892 | 1.4:+82681128 | MS.gene71661:CDS |
| AGATTCACAGAGAACACCAT+TGG | 0.603261 | 1.4:+82679357 | MS.gene71661:CDS |
| GATGCTGTAGCTTTAATCAA+GGG | 0.604486 | 1.4:+82679262 | MS.gene71661:CDS |
| TCCACGTTCGGGACATCGGA+TGG | 0.605166 | 1.4:+82672887 | MS.gene71661:CDS |
| ATGATACACTTAGAGAAGTG+AGG | 0.607586 | 1.4:+82673455 | MS.gene71661:CDS |
| CTATTCAAAAGAAGTTTCAT+CGG | 0.608107 | 1.4:+82674798 | MS.gene71661:CDS |
| TGGACGCATTAATTCCTGCA+TGG | 0.609998 | 1.4:+82676240 | MS.gene71661:CDS |
| AGAGAGGTTCCATCACTACA+AGG | 0.611220 | 1.4:+82672692 | MS.gene71661:CDS |
| TGAAAACCACTACGCCCAGT+TGG | 0.612932 | 1.4:-82674543 | None:intergenic |
| ACGCTTAAAGAAATCTCTCA+AGG | 0.614690 | 1.4:-82679599 | None:intergenic |
| TCTGTGAATCTGACAAGCCA+AGG | 0.615736 | 1.4:-82679345 | None:intergenic |
| ATCTGCGTAGGAAAGAAAGA+AGG | 0.619000 | 1.4:+82679296 | MS.gene71661:CDS |
| CATGTGTGTTCATAAGAGGA+GGG | 0.619549 | 1.4:+82675087 | MS.gene71661:CDS |
| TGAATGGGTGGAAGTTTCAG+AGG | 0.621213 | 1.4:+82677346 | MS.gene71661:CDS |
| ATGTGGCCAACTGGGCGTAG+TGG | 0.621719 | 1.4:+82674537 | MS.gene71661:CDS |
| CCTCCTCTTATGAACACACA+TGG | 0.626591 | 1.4:-82675086 | None:intergenic |
| GTTGTGGATATAGAAGTTGA+AGG | 0.627219 | 1.4:+82675127 | MS.gene71661:CDS |
| ACCATCCGATGTCCCGAACG+TGG | 0.627885 | 1.4:-82672888 | None:intergenic |
| TACTGCAAGAGATGAAGCTG+AGG | 0.628207 | 1.4:+82675964 | MS.gene71661:CDS |
| GAAACACTTACCAAACCTGA+AGG | 0.628495 | 1.4:+82676061 | MS.gene71661:CDS |
| TGGACCTGAAAACTAATCAG+TGG | 0.633743 | 1.4:+82672835 | MS.gene71661:CDS |
| GCTGTAGCTTTAATCAAGGG+AGG | 0.634285 | 1.4:+82679265 | MS.gene71661:CDS |
| GGACCTGAAAACTAATCAGT+GGG | 0.635849 | 1.4:+82672836 | MS.gene71661:CDS |
| TTACATCTCCAAATCAAGAG+AGG | 0.638987 | 1.4:+82672676 | MS.gene71661:CDS |
| CAGGAATTAATGCGTCCACA+GGG | 0.639638 | 1.4:-82676235 | None:intergenic |
| CACATGGAAAACCCTGCACG+AGG | 0.650088 | 1.4:-82675070 | None:intergenic |
| ATGATGTTGAGAAGCTGGAG+TGG | 0.650953 | 1.4:+82672472 | MS.gene71661:CDS |
| GCTTGATGAGCACTGCGGGG+AGG | 0.652275 | 1.4:-82672522 | None:intergenic |
| TAATAGTGATGGTACAAGTG+AGG | 0.660212 | 1.4:+82677412 | MS.gene71661:CDS |
| GATACACTATATATATATGG+TGG | 0.661154 | 1.4:+82676274 | MS.gene71661:CDS |
| GAGGGGTAAAGAATCTGCGT+AGG | 0.665128 | 1.4:+82679284 | MS.gene71661:CDS |
| CTGAATGCATCAGATCAAGT+GGG | 0.666302 | 1.4:+82679238 | MS.gene71661:CDS |
| GATGATGAATCTAATAGTGA+TGG | 0.667183 | 1.4:+82677401 | MS.gene71661:CDS |
| GTAGGAAAGAAAGAAGGTCA+CGG | 0.670544 | 1.4:+82679302 | MS.gene71661:CDS |
| AACCCTGCACGAGGACCAGG+AGG | 0.671017 | 1.4:-82675061 | None:intergenic |
| ATGAACAATTCCTTTCTCAG+AGG | 0.672557 | 1.4:-82674830 | None:intergenic |
| CAACAGCTTGATGAGCACTG+CGG | 0.679746 | 1.4:-82672527 | None:intergenic |
| TTCTAGGCAGTGAAACCCTG+TGG | 0.688866 | 1.4:+82676220 | MS.gene71661:intron |
| TGGGTGATAGGAACTCCGCA+AGG | 0.694460 | 1.4:+82681095 | MS.gene71661:intron |
| GCTAAAACCTATTCTTGACG+AGG | 0.698872 | 1.4:+82681152 | MS.gene71661:CDS |
| CAAGATCAAAACCATCCTTG+CGG | 0.699328 | 1.4:-82681110 | None:intergenic |
| ACAGCTTGATGAGCACTGCG+GGG | 0.699986 | 1.4:-82672525 | None:intergenic |
| AGCCTAAGCCTGGAGCAATG+TGG | 0.720894 | 1.4:+82674520 | MS.gene71661:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 82672438 | 82681173 | 82672438 | ID=MS.gene71661 |
| chr1.4 | mRNA | 82672438 | 82681173 | 82672438 | ID=MS.gene71661.t1;Parent=MS.gene71661 |
| chr1.4 | exon | 82672438 | 82672576 | 82672438 | ID=MS.gene71661.t1.exon1;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82672438 | 82672576 | 82672438 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82672667 | 82672713 | 82672667 | ID=MS.gene71661.t1.exon2;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82672667 | 82672713 | 82672667 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82672822 | 82672908 | 82672822 | ID=MS.gene71661.t1.exon3;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82672822 | 82672908 | 82672822 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82673415 | 82673476 | 82673415 | ID=MS.gene71661.t1.exon4;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82673415 | 82673476 | 82673415 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82674311 | 82674353 | 82674311 | ID=MS.gene71661.t1.exon5;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82674311 | 82674353 | 82674311 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82674507 | 82674587 | 82674507 | ID=MS.gene71661.t1.exon6;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82674507 | 82674587 | 82674507 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82674781 | 82674897 | 82674781 | ID=MS.gene71661.t1.exon7;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82674781 | 82674897 | 82674781 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82675043 | 82675148 | 82675043 | ID=MS.gene71661.t1.exon8;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82675043 | 82675148 | 82675043 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82675486 | 82675549 | 82675486 | ID=MS.gene71661.t1.exon9;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82675486 | 82675549 | 82675486 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82675759 | 82675801 | 82675759 | ID=MS.gene71661.t1.exon10;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82675759 | 82675801 | 82675759 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82675905 | 82676135 | 82675905 | ID=MS.gene71661.t1.exon11;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82675905 | 82676135 | 82675905 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82676226 | 82676387 | 82676226 | ID=MS.gene71661.t1.exon12;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82676226 | 82676387 | 82676226 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82677335 | 82677466 | 82677335 | ID=MS.gene71661.t1.exon13;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82677335 | 82677466 | 82677335 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82679235 | 82679378 | 82679235 | ID=MS.gene71661.t1.exon14;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82679235 | 82679378 | 82679235 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82679589 | 82679675 | 82679589 | ID=MS.gene71661.t1.exon15;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82679589 | 82679675 | 82679589 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| chr1.4 | exon | 82681105 | 82681173 | 82681105 | ID=MS.gene71661.t1.exon16;Parent=MS.gene71661.t1 |
| chr1.4 | CDS | 82681105 | 82681173 | 82681105 | ID=cds.MS.gene71661.t1;Parent=MS.gene71661.t1 |
| Gene Sequence |
| Protein sequence |
