AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71685


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71685.t1 MTR_1g107445 97.207 179 5 0 2 180 26 204 1.15e-124 349
MS.gene71685.t1 MTR_8g099060 35.754 179 112 1 3 178 80 258 1.90e-31 114
MS.gene71685.t1 MTR_8g028450 32.222 180 118 1 2 177 86 265 3.80e-25 98.2
MS.gene71685.t1 MTR_4g014700 39.326 178 106 1 4 179 31 208 5.05e-24 94.0
MS.gene71685.t1 MTR_1g099500 32.749 171 112 1 3 170 73 243 2.12e-23 93.2
MS.gene71685.t1 MTR_3g449880 33.951 162 104 1 3 161 65 226 6.45e-23 91.7
MS.gene71685.t1 MTR_3g449880 33.951 162 104 1 3 161 43 204 7.20e-23 91.3
MS.gene71685.t1 MTR_5g018250 27.273 176 123 1 10 180 49 224 2.36e-21 87.4
MS.gene71685.t1 MTR_6g012460 32.386 176 119 0 3 178 22 197 4.21e-21 86.3
MS.gene71685.t1 MTR_1g109630 27.545 167 117 1 14 180 44 206 6.98e-20 83.2
MS.gene71685.t1 MTR_5g090340 31.694 183 116 3 3 180 109 287 1.05e-19 84.3
MS.gene71685.t1 MTR_5g090340 31.694 183 116 3 3 180 112 290 1.08e-19 84.3
MS.gene71685.t1 MTR_5g018080 29.457 129 91 0 6 134 27 155 5.18e-18 77.0
MS.gene71685.t1 MTR_5g018190 29.851 134 94 0 10 143 296 429 2.97e-17 78.6
MS.gene71685.t1 MTR_5g018110 27.907 129 93 0 6 134 353 481 1.20e-14 71.2
MS.gene71685.t1 MTR_6g012460 35.246 122 79 0 3 124 22 143 9.32e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71685.t1 AT3G19460 53.591 181 73 2 3 180 28 200 2.35e-57 179
MS.gene71685.t1 AT3G19460 53.591 181 73 2 3 180 28 200 2.35e-57 179
MS.gene71685.t1 AT3G19460 53.591 181 73 2 3 180 28 200 2.35e-57 179
MS.gene71685.t1 AT2G15280 48.333 180 92 1 2 180 22 201 1.09e-55 174
MS.gene71685.t1 AT3G19460 45.581 215 72 3 3 180 12 218 3.30e-52 166
MS.gene71685.t1 AT3G19460 45.581 215 72 3 3 180 12 218 3.30e-52 166
MS.gene71685.t1 AT3G19460 45.581 215 72 3 3 180 28 234 4.96e-52 166
MS.gene71685.t1 AT3G19460 45.581 215 72 3 3 180 28 234 4.96e-52 166
MS.gene71685.t1 AT2G15280 38.333 180 71 2 2 180 22 162 3.09e-35 121
MS.gene71685.t1 AT4G23630 36.932 176 105 3 5 178 102 273 8.06e-29 108
MS.gene71685.t1 AT4G23630 36.932 176 105 3 5 178 102 273 8.06e-29 108
MS.gene71685.t1 AT2G46170 35.758 165 106 0 13 177 87 251 6.26e-27 102
MS.gene71685.t1 AT1G64090 34.831 178 109 2 3 175 70 245 1.12e-26 102
MS.gene71685.t1 AT5G41600 32.973 185 113 3 3 178 74 256 1.14e-26 102
MS.gene71685.t1 AT1G64090 34.857 175 107 2 3 172 86 258 1.41e-26 102
MS.gene71685.t1 AT4G11220 31.609 174 117 1 5 178 98 269 1.73e-26 102
MS.gene71685.t1 AT3G10915 32.584 178 117 1 3 177 14 191 3.20e-26 99.4
MS.gene71685.t1 AT3G10915 32.402 179 118 1 3 178 15 193 3.72e-26 99.4
MS.gene71685.t1 AT3G61560 33.708 178 115 1 3 177 74 251 4.64e-26 100
MS.gene71685.t1 AT3G61560 33.708 178 115 1 3 177 74 251 4.64e-26 100
MS.gene71685.t1 AT3G10915 33.136 169 113 0 10 178 57 225 6.02e-26 99.4
MS.gene71685.t1 AT3G10915 33.333 168 112 0 10 177 82 249 1.13e-25 99.4
MS.gene71685.t1 AT3G10915 33.529 170 112 1 10 178 57 226 4.69e-25 97.1
MS.gene71685.t1 AT3G10915 33.728 169 111 1 10 177 79 247 9.10e-25 97.1
MS.gene71685.t1 AT3G18260 29.609 179 123 1 3 178 45 223 8.57e-24 94.0
MS.gene71685.t1 AT3G10260 32.240 183 117 4 3 180 67 247 1.58e-22 90.9
MS.gene71685.t1 AT3G10260 32.240 183 117 4 3 180 67 247 1.58e-22 90.9
MS.gene71685.t1 AT3G10260 32.240 183 117 4 3 180 87 267 1.99e-22 91.3
MS.gene71685.t1 AT3G54120 41.216 148 80 2 1 143 28 173 4.11e-22 89.0
MS.gene71685.t1 AT3G61560 37.302 126 76 1 3 125 74 199 2.06e-19 82.0
MS.gene71685.t1 AT4G01230 33.333 174 106 6 3 171 74 242 9.33e-15 70.1
MS.gene71685.t1 AT4G01230 33.333 174 106 6 3 171 76 244 1.05e-14 70.1
MS.gene71685.t1 AT2G23640 22.599 177 133 1 8 180 30 206 2.35e-13 65.9
MS.gene71685.t1 AT3G10915 32.979 94 63 0 10 103 57 150 1.24e-11 60.5
MS.gene71685.t1 AT2G46170 34.524 84 55 0 13 96 87 170 4.41e-11 59.3

Find 35 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTGATGTACTGCAAATT+TGG 0.179244 1.1:+77149284 MS.gene71685:CDS
TTGTTGTGATTCTGTTTCTA+TGG 0.238941 1.1:+77148780 MS.gene71685:CDS
TCTGCCTGATCTGGAGATTT+CGG 0.363024 1.1:+77149243 MS.gene71685:CDS
ACTTCCTCCTCTGCCTGATC+TGG 0.365713 1.1:+77149234 MS.gene71685:CDS
ACTGCAAATTTGGCTAAATC+AGG 0.373614 1.1:+77149294 MS.gene71685:CDS
AAGCTGCTAATCTTCTTAAC+AGG 0.392636 1.1:+77148807 MS.gene71685:CDS
CACATGATATAGCAATTAAC+AGG 0.396290 1.1:+77149329 MS.gene71685:CDS
TTGTTGACAATATCTTATAT+TGG 0.407398 1.1:+77149494 MS.gene71685:CDS
TTGGCTAAATCAGGCTTTGT+CGG 0.437383 1.1:+77149303 MS.gene71685:CDS
ACTTGGTGAATCAGGTTGTT+GGG 0.454940 1.1:+77149468 MS.gene71685:intron
TTCTCCGAAATCTCCAGATC+AGG 0.456361 1.1:-77149247 None:intergenic
AAACGATAAGAAATTGTAAC+CGG 0.457016 1.1:-77148735 None:intergenic
TGTTGTGATTCTGTTTCTAT+GGG 0.474872 1.1:+77148781 MS.gene71685:CDS
TGGTATCTGTTCGAGCGAGC+CGG 0.484056 1.1:+77148716 MS.gene71685:CDS
TACATATGTAGATCAAAGTC+AGG 0.491717 1.1:-77149532 None:intergenic
AGATCAGGCAGAGGAGGAAG+TGG 0.496919 1.1:-77149232 None:intergenic
CAAAGAAAAGAAAGTGCAGT+AGG 0.516683 1.1:+77149800 MS.gene71685:CDS
AACTTGGTGAATCAGGTTGT+TGG 0.517512 1.1:+77149467 MS.gene71685:intron
TCTTCTCATCAATTCGATCC+TGG 0.524568 1.1:-77149695 None:intergenic
ATCTCCAGATCAGGCAGAGG+AGG 0.526888 1.1:-77149238 None:intergenic
CACAGTATCAAAAGATTCGC+AGG 0.529807 1.1:+77149745 MS.gene71685:CDS
GAATTTGTTTCTCTGTGCAC+AGG 0.543756 1.1:+77149351 MS.gene71685:CDS
GAGAAGATTCATGTGGTACA+TGG 0.548698 1.1:+77149711 MS.gene71685:CDS
CTTGGTGAATCAGGTTGTTG+GGG 0.553448 1.1:+77149469 MS.gene71685:intron
CTGGTACTTGTCATATGCCA+CGG 0.562166 1.1:-77149676 None:intergenic
CGTGGCATATGACAAGTACC+AGG 0.566409 1.1:+77149677 MS.gene71685:CDS
TCTTAGCTTGTCTCTTCCCG+TGG 0.595833 1.1:+77149659 MS.gene71685:CDS
TGGTACTTGTCATATGCCAC+GGG 0.597209 1.1:-77149675 None:intergenic
AGAAGATTCATGTGGTACAT+GGG 0.608693 1.1:+77149712 MS.gene71685:CDS
TGATTTCTGCAACCACATTG+TGG 0.618418 1.1:+77148696 MS.gene71685:CDS
CTATGGAAGAATTGGCGTGG+AGG 0.619260 1.1:+77148662 MS.gene71685:intron
GAAATCTCCAGATCAGGCAG+AGG 0.635921 1.1:-77149241 None:intergenic
TTGCTATGGAAGAATTGGCG+TGG 0.691195 1.1:+77148659 MS.gene71685:intron
AATTGATGAGAAGATTCATG+TGG 0.715992 1.1:+77149704 MS.gene71685:CDS
CTCGAACAGATACCACAATG+TGG 0.752392 1.1:-77148708 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATAATTTCAATCAT+AGG - chr1.1:77149199-77149218 None:intergenic 10.0%
!! TTCTATCTTGAAAACAAAAT+CGG + chr1.1:77148591-77148610 MS.gene71685:intron 20.0%
!! TTGTTGACAATATCTTATAT+TGG + chr1.1:77149494-77149513 MS.gene71685:CDS 20.0%
!!! AATTGACCTTGTTTATAATT+CGG + chr1.1:77148554-77148573 MS.gene71685:intron 20.0%
! AAACGATAAGAAATTGTAAC+CGG - chr1.1:77148738-77148757 None:intergenic 25.0%
! ACGAGATTAATTGGAAATTT+AGG - chr1.1:77148886-77148905 None:intergenic 25.0%
! AGTGTAAGTAAGAAAACTAA+AGG - chr1.1:77148530-77148549 None:intergenic 25.0%
! ATTTCTGTAATGATCATTTC+AGG + chr1.1:77149630-77149649 MS.gene71685:intron 25.0%
! TGTTGAAGATTGAAAGAAAT+CGG - chr1.1:77148504-77148523 None:intergenic 25.0%
! TTTGTTTATGTTGAGTTCTA+TGG + chr1.1:77149399-77149418 MS.gene71685:intron 25.0%
AATTGATGAGAAGATTCATG+TGG + chr1.1:77149704-77149723 MS.gene71685:CDS 30.0%
CACATGATATAGCAATTAAC+AGG + chr1.1:77149329-77149348 MS.gene71685:CDS 30.0%
CATGAACCGAATTATAAACA+AGG - chr1.1:77148563-77148582 None:intergenic 30.0%
TACATATGTAGATCAAAGTC+AGG - chr1.1:77149535-77149554 None:intergenic 30.0%
TTTCTGATGTACTGCAAATT+TGG + chr1.1:77149284-77149303 MS.gene71685:CDS 30.0%
! TTTTCTTTGCTTGAACGATT+TGG - chr1.1:77149789-77149808 None:intergenic 30.0%
!! TGTTGTGATTCTGTTTCTAT+GGG + chr1.1:77148781-77148800 MS.gene71685:CDS 30.0%
!! TTGTTGTGATTCTGTTTCTA+TGG + chr1.1:77148780-77148799 MS.gene71685:CDS 30.0%
AAGCTGCTAATCTTCTTAAC+AGG + chr1.1:77148807-77148826 MS.gene71685:CDS 35.0%
ACTGCAAATTTGGCTAAATC+AGG + chr1.1:77149294-77149313 MS.gene71685:CDS 35.0%
AGAAGATTCATGTGGTACAT+GGG + chr1.1:77149712-77149731 MS.gene71685:CDS 35.0%
CCAATTAATCTCGTTTGAGA+GGG + chr1.1:77148892-77148911 MS.gene71685:intron 35.0%
GAAATTCTCTCTGTGTAAGT+TGG + chr1.1:77149600-77149619 MS.gene71685:intron 35.0%
GAAGATTGAAAGAAATCGGA+AGG - chr1.1:77148500-77148519 None:intergenic 35.0%
GACACTTGTTTGTATGAACT+TGG + chr1.1:77149451-77149470 MS.gene71685:intron 35.0%
TCCAATTAATCTCGTTTGAG+AGG + chr1.1:77148891-77148910 MS.gene71685:intron 35.0%
! ATGTGTTGCTATGGAAGAAT+TGG + chr1.1:77148654-77148673 MS.gene71685:intron 35.0%
! TGCGAATCTTTTGATACTGT+GGG - chr1.1:77149746-77149765 None:intergenic 35.0%
! TTGTATGAACTTGGTGAATC+AGG + chr1.1:77149460-77149479 MS.gene71685:intron 35.0%
CACAGTATCAAAAGATTCGC+AGG + chr1.1:77149745-77149764 MS.gene71685:CDS 40.0%
CCCTCTCAAACGAGATTAAT+TGG - chr1.1:77148895-77148914 None:intergenic 40.0%
GAGAAGATTCATGTGGTACA+TGG + chr1.1:77149711-77149730 MS.gene71685:CDS 40.0%
TCTTCTCATCAATTCGATCC+TGG - chr1.1:77149698-77149717 None:intergenic 40.0%
TGATGAACGGAAATTCGTTG+AGG - chr1.1:77148432-77148451 None:intergenic 40.0%
TGATTTCTGCAACCACATTG+TGG + chr1.1:77148696-77148715 MS.gene71685:CDS 40.0%
! AACTTGGTGAATCAGGTTGT+TGG + chr1.1:77149467-77149486 MS.gene71685:intron 40.0%
! ACTTGGTGAATCAGGTTGTT+GGG + chr1.1:77149468-77149487 MS.gene71685:intron 40.0%
! CTGCGAATCTTTTGATACTG+TGG - chr1.1:77149747-77149766 None:intergenic 40.0%
! GAATTTGTTTCTCTGTGCAC+AGG + chr1.1:77149351-77149370 MS.gene71685:CDS 40.0%
! TAGTTGCTGATGTGTTGCTA+TGG + chr1.1:77148645-77148664 MS.gene71685:intron 40.0%
! TTGGCTAAATCAGGCTTTGT+CGG + chr1.1:77149303-77149322 MS.gene71685:CDS 40.0%
ATTTCCGTTCATCACGCTCT+CGG + chr1.1:77148438-77148457 MS.gene71685:intron 45.0%
CTCGAACAGATACCACAATG+TGG - chr1.1:77148711-77148730 None:intergenic 45.0%
CTGGTACTTGTCATATGCCA+CGG - chr1.1:77149679-77149698 None:intergenic 45.0%
TCTGCCTGATCTGGAGATTT+CGG + chr1.1:77149243-77149262 MS.gene71685:CDS 45.0%
TGGTACTTGTCATATGCCAC+GGG - chr1.1:77149678-77149697 None:intergenic 45.0%
TTCTCCGAAATCTCCAGATC+AGG - chr1.1:77149250-77149269 None:intergenic 45.0%
TTGCTATGGAAGAATTGGCG+TGG + chr1.1:77148659-77148678 MS.gene71685:intron 45.0%
! CTTGGTGAATCAGGTTGTTG+GGG + chr1.1:77149469-77149488 MS.gene71685:intron 45.0%
AATCGGAAGGAGAGAGAGAG+AGG - chr1.1:77148487-77148506 None:intergenic 50.0%
ATCGGAAGGAGAGAGAGAGA+GGG - chr1.1:77148486-77148505 None:intergenic 50.0%
CGTGGCATATGACAAGTACC+AGG + chr1.1:77149677-77149696 MS.gene71685:CDS 50.0%
CTATGGAAGAATTGGCGTGG+AGG + chr1.1:77148662-77148681 MS.gene71685:intron 50.0%
GAAATCTCCAGATCAGGCAG+AGG - chr1.1:77149244-77149263 None:intergenic 50.0%
!! TCTTAGCTTGTCTCTTCCCG+TGG + chr1.1:77149659-77149678 MS.gene71685:CDS 50.0%
ACTTCCTCCTCTGCCTGATC+TGG + chr1.1:77149234-77149253 MS.gene71685:CDS 55.0%
AGATCAGGCAGAGGAGGAAG+TGG - chr1.1:77149235-77149254 None:intergenic 55.0%
ATCTCCAGATCAGGCAGAGG+AGG - chr1.1:77149241-77149260 None:intergenic 55.0%
TGGTATCTGTTCGAGCGAGC+CGG + chr1.1:77148716-77148735 MS.gene71685:CDS 55.0%
! AGAGCCGAGAGCGTGATGAA+CGG - chr1.1:77148445-77148464 None:intergenic 55.0%
GTTCATCACGCTCTCGGCTC+TGG + chr1.1:77148444-77148463 MS.gene71685:intron 60.0%
! CACGCTCTCGGCTCTGGCTT+AGG + chr1.1:77148450-77148469 MS.gene71685:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 77148420 77149821 77148420 ID=MS.gene71685
chr1.1 mRNA 77148420 77149821 77148420 ID=MS.gene71685.t1;Parent=MS.gene71685
chr1.1 exon 77148420 77148422 77148420 ID=MS.gene71685.t1.exon1;Parent=MS.gene71685.t1
chr1.1 CDS 77148420 77148422 77148420 ID=cds.MS.gene71685.t1;Parent=MS.gene71685.t1
chr1.1 exon 77148671 77148828 77148671 ID=MS.gene71685.t1.exon2;Parent=MS.gene71685.t1
chr1.1 CDS 77148671 77148828 77148671 ID=cds.MS.gene71685.t1;Parent=MS.gene71685.t1
chr1.1 exon 77149231 77149372 77149231 ID=MS.gene71685.t1.exon3;Parent=MS.gene71685.t1
chr1.1 CDS 77149231 77149372 77149231 ID=cds.MS.gene71685.t1;Parent=MS.gene71685.t1
chr1.1 exon 77149482 77149551 77149482 ID=MS.gene71685.t1.exon4;Parent=MS.gene71685.t1
chr1.1 CDS 77149482 77149551 77149482 ID=cds.MS.gene71685.t1;Parent=MS.gene71685.t1
chr1.1 exon 77149652 77149821 77149652 ID=MS.gene71685.t1.exon5;Parent=MS.gene71685.t1
chr1.1 CDS 77149652 77149821 77149652 ID=cds.MS.gene71685.t1;Parent=MS.gene71685.t1
Gene Sequence

>MS.gene71685

ATGGTAGAACCTCAACGAATTTCCGTTCATCACGCTCTCGGCTCTGGCTTAGGTTCTCTTCTTCCCTCTCTCTCTCTCCTTCCGATTTCTTTCAATCTTCAACAGATCCTTTAGTTTTCTTACTTACACTTTACAATTGACCTTGTTTATAATTCGGTTCATGCGATTGATTTCTATCTTGAAAACAAAATCGGAGTTGACTAATTTTTTTATTTGATTTGCGTTTAGTTGCTGATGTGTTGCTATGGAAGAATTGGCGTGGAGGAGTTACGACTCTGATTTCTGCAACCACATTGTGGTATCTGTTCGAGCGAGCCGGTTACAATTTCTTATCGTTTGTTGCTAATGTGATATTGCTTCTTGTTGTGATTCTGTTTCTATGGGCGAAAGCTGCTAATCTTCTTAACAGGTATTTCATTTCTTCATTATCATTTTGTATTTTCATCAAGATTCTTTGTTCAGACCTAAATTTCCAATTAATCTCGTTTGAGAGGGAAAATTAGTGCATATAGATGCTATATTAAGTGTTCTAAGACTGATTCTTGACTCTTACATAGAATTAGAAAGAGATTTCATCATGAATTAATTTTCACGAATTGTCAGTGTAAAAAGCAAATCACAATCGATCATGTGTGTGACTTTTCTTTTGATCTAAATTGACAGTGCATGTGAATTTTCTTTTGATCTAAACTGACAGTGTATATGAATTAAATCATTTTATTATACATGTTATACAGAAAGAAATTTTTTAGATCTGTTACTATAAGTCTATACTTCCTATGATTGAAATTATTTTATTTTTATGTTACAGACCACTTCCTCCTCTGCCTGATCTGGAGATTTCGGAGAAAACTATTGCGAAGCTTTCTGATGTACTGCAAATTTGGCTAAATCAGGCTTTGTCGGTTGCACATGATATAGCAATTAACAGGAATTTGTTTCTCTGTGCACAGGTACTGTGATGTATCTGCTTCTTCTCTTTGTTTATGTTGAGTTCTATGGATTTGTCGAGTATAGACATTTTTTACATTGACACTTGTTTGTATGAACTTGGTGAATCAGGTTGTTGGGGTGTTGTTGACAATATCTTATATTGGTAGTCTGTTTAACTTCCTGACTTTGATCTACATATGTAAGTTGCATAATTCTGTAGCTTTTATGCGCTCTTATATATGTTTTTGAAATTCTCTCTGTGTAAGTTGGTTTTAATATTTCTGTAATGATCATTTCAGGTGTTCTTCTTAGCTTGTCTCTTCCCGTGGCATATGACAAGTACCAGGATCGAATTGATGAGAAGATTCATGTGGTACATGGGATTATTCACCCACAGTATCAAAAGATTCGCAGGATTGTTTTGAGCATTATTCCAAATCGTTCAAGCAAAGAAAAGAAAGTGCAGTAG

Protein sequence

>MS.gene71685.t1

MNWRGGVTTLISATTLWYLFERAGYNFLSFVANVILLLVVILFLWAKAANLLNRPLPPLPDLEISEKTIAKLSDVLQIWLNQALSVAHDIAINRNLFLCAQVVGVLLTISYIGSLFNFLTLIYICVLLSLSLPVAYDKYQDRIDEKIHVVHGIIHPQYQKIRRIVLSIIPNRSSKEKKVQ