AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72105

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72105.t1 MTR_4g101990 92.537 201 4 1 13 202 132 332 2.66e-130 369
MS.gene72105.t1 MTR_7g074935 57.547 212 58 5 20 200 122 332 9.05e-56 180
MS.gene72105.t1 MTR_8g036160 50.000 226 75 3 13 200 130 355 5.57e-52 171
MS.gene72105.t1 MTR_1g076710 61.589 151 46 3 13 152 122 271 1.23e-45 154
MS.gene72105.t1 MTR_1g061530 51.948 154 60 4 13 154 129 280 5.42e-43 147
MS.gene72105.t1 MTR_5g011610 56.000 150 47 3 25 161 56 199 1.23e-40 140
MS.gene72105.t1 MTR_5g011610 49.057 106 39 2 15 109 202 303 5.12e-20 86.3
MS.gene72105.t1 MTR_5g011680 55.333 150 50 4 20 154 137 284 2.38e-40 139
MS.gene72105.t1 MTR_6g061670 55.474 137 46 3 21 146 121 253 4.58e-37 131
MS.gene72105.t1 MTR_6g061670 55.474 137 46 3 21 146 126 258 5.67e-37 131
MS.gene72105.t1 MTR_5g011620 54.248 153 53 2 20 161 110 256 2.70e-36 134
MS.gene72105.t1 MTR_5g010330 48.980 147 59 4 13 148 122 263 1.51e-34 124
MS.gene72105.t1 MTR_8g098390 48.592 142 60 3 13 143 154 293 2.28e-32 119
MS.gene72105.t1 MTR_8g031100 51.449 138 55 2 17 143 147 283 1.12e-31 117
MS.gene72105.t1 MTR_7g077120 44.444 153 72 3 13 153 145 296 1.06e-29 112
MS.gene72105.t1 MTR_4g098460 54.545 121 42 3 22 131 78 196 3.09e-29 110
MS.gene72105.t1 MTR_4g055803 47.794 136 54 5 20 139 120 254 7.80e-28 108
MS.gene72105.t1 MTR_4g098460 52.381 126 42 4 22 131 78 201 6.91e-27 103
MS.gene72105.t1 MTR_1g079780 50.847 118 42 3 25 130 109 222 7.55e-27 104
MS.gene72105.t1 MTR_4g055813 47.287 129 51 4 25 139 19 144 1.68e-26 102
MS.gene72105.t1 MTR_4g055833 47.059 136 55 5 20 139 117 251 1.27e-25 101
MS.gene72105.t1 MTR_5g011600 43.548 124 60 2 66 188 3 117 2.01e-23 90.5
MS.gene72105.t1 MTR_7g116320 47.287 129 53 2 20 137 138 262 2.25e-23 95.9
MS.gene72105.t1 MTR_4g055705 40.559 143 71 3 1 129 112 254 4.92e-22 91.3
MS.gene72105.t1 MTR_6g060670 45.385 130 56 3 20 139 172 296 1.12e-21 90.5
MS.gene72105.t1 MTR_7g116320 47.899 119 47 2 20 127 138 252 5.03e-21 88.2
MS.gene72105.t1 MTR_5g041430 41.129 124 57 4 16 126 102 222 2.88e-18 81.3
MS.gene72105.t1 MTR_1g038960 43.802 121 52 4 22 130 147 263 3.17e-17 79.0
MS.gene72105.t1 MTR_3g116100 45.833 96 47 3 42 137 171 261 4.45e-16 75.5
MS.gene72105.t1 MTR_3g023560 38.168 131 60 4 17 130 51 177 9.24e-16 73.6
MS.gene72105.t1 MTR_3g100470 42.268 97 54 2 42 137 129 224 1.50e-15 73.6
MS.gene72105.t1 MTR_3g068035 41.912 136 72 3 5 140 99 227 1.84e-15 73.6
MS.gene72105.t1 MTR_5g014360 40.496 121 56 4 22 130 187 303 4.62e-15 72.8
MS.gene72105.t1 MTR_5g011700 47.191 89 43 2 42 130 28 112 1.55e-14 69.3
MS.gene72105.t1 MTR_5g013390 38.017 121 59 4 22 130 145 261 2.99e-14 70.5
MS.gene72105.t1 MTR_1g038990 40.000 125 58 5 18 130 124 243 6.25e-14 69.3
MS.gene72105.t1 MTR_5g066020 36.800 125 63 4 26 137 81 202 1.16e-13 67.8
MS.gene72105.t1 MTR_5g091630 37.594 133 62 2 32 147 92 220 1.96e-12 64.3
MS.gene72105.t1 MTR_4g098450 47.253 91 44 1 40 130 129 215 3.10e-12 64.3
MS.gene72105.t1 MTR_7g080980 42.982 114 50 2 28 130 87 196 1.19e-11 62.4
MS.gene72105.t1 MTR_1g044155 43.564 101 52 2 40 140 179 274 2.32e-11 62.0
MS.gene72105.t1 MTR_1g073860 46.154 91 45 1 40 130 147 233 4.71e-11 61.2
MS.gene72105.t1 MTR_5g011520 42.857 98 52 1 33 130 146 239 4.91e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72105.t1 AT4G12080 65.842 202 50 7 13 199 158 355 4.29e-78 238
MS.gene72105.t1 AT4G22770 57.286 199 70 4 13 199 138 333 1.03e-55 180
MS.gene72105.t1 AT4G22770 57.286 199 70 4 13 199 138 333 1.03e-55 180
MS.gene72105.t1 AT4G25320 66.667 129 31 2 21 138 162 289 4.53e-50 167
MS.gene72105.t1 AT5G51590 68.548 124 27 2 21 133 174 296 1.04e-46 159
MS.gene72105.t1 AT5G62260 60.870 138 38 3 19 141 154 290 1.26e-46 158
MS.gene72105.t1 AT5G62260 60.870 138 38 3 19 141 154 290 1.26e-46 158
MS.gene72105.t1 AT4G00200 70.896 134 26 3 23 144 121 253 2.16e-45 153
MS.gene72105.t1 AT4G00200 70.896 134 26 3 23 144 121 253 2.16e-45 153
MS.gene72105.t1 AT2G33620 54.545 165 56 4 20 173 158 314 2.44e-43 148
MS.gene72105.t1 AT2G33620 54.545 165 56 4 20 173 158 314 2.44e-43 148
MS.gene72105.t1 AT2G33620 54.545 165 56 4 20 173 158 314 2.44e-43 148
MS.gene72105.t1 AT2G33620 54.545 165 56 4 20 173 158 314 2.44e-43 148
MS.gene72105.t1 AT5G62260 60.484 124 37 2 19 131 154 276 1.44e-42 145
MS.gene72105.t1 AT2G45850 53.125 128 48 2 15 131 152 278 4.85e-36 129
MS.gene72105.t1 AT2G45850 53.125 128 48 2 15 131 152 278 4.85e-36 129
MS.gene72105.t1 AT1G63470 51.515 132 51 3 13 133 165 294 1.39e-34 126
MS.gene72105.t1 AT1G63480 48.252 143 61 3 13 144 142 282 2.00e-33 122
MS.gene72105.t1 AT1G63480 48.252 143 61 3 13 144 142 282 2.00e-33 122
MS.gene72105.t1 AT1G63480 48.252 143 61 3 13 144 142 282 2.00e-33 122
MS.gene72105.t1 AT1G63480 48.252 143 61 3 13 144 142 282 2.00e-33 122
MS.gene72105.t1 AT1G63480 48.252 143 61 3 13 144 142 282 2.00e-33 122
MS.gene72105.t1 AT1G63480 48.252 143 61 3 13 144 148 288 2.27e-33 122
MS.gene72105.t1 AT1G63480 48.252 143 61 3 13 144 148 288 2.27e-33 122
MS.gene72105.t1 AT2G45850 52.459 122 46 2 15 125 152 272 1.10e-32 119
MS.gene72105.t1 AT4G17950 47.619 147 63 3 22 155 218 363 1.97e-32 121
MS.gene72105.t1 AT3G61310 51.538 130 51 2 13 131 152 280 3.46e-31 116
MS.gene72105.t1 AT3G04590 46.721 122 50 2 21 131 167 284 3.64e-29 110
MS.gene72105.t1 AT3G04590 46.721 122 50 2 21 131 167 284 3.11e-28 109
MS.gene72105.t1 AT5G46640 51.908 131 48 3 22 141 177 303 1.07e-25 102
MS.gene72105.t1 AT2G36560 40.496 121 60 2 22 130 102 222 1.37e-19 86.3
MS.gene72105.t1 AT3G60870 47.253 91 42 2 40 130 112 196 2.83e-15 72.4
MS.gene72105.t1 AT5G49700 39.815 108 61 3 42 145 111 218 2.26e-14 70.1
MS.gene72105.t1 AT2G42940 51.087 92 41 1 40 131 106 193 6.05e-14 68.9
MS.gene72105.t1 AT1G14490 41.284 109 56 3 42 142 77 185 1.64e-13 67.0
MS.gene72105.t1 AT1G14490 41.284 109 56 3 42 142 58 166 1.72e-13 66.6
MS.gene72105.t1 AT4G17800 46.154 91 45 1 40 130 135 221 2.30e-11 62.0
MS.gene72105.t1 AT4G14465 37.324 142 82 3 5 146 87 221 6.33e-11 60.5
MS.gene72105.t1 AT2G35270 41.121 107 58 2 40 146 131 232 6.36e-11 60.5
MS.gene72105.t1 AT3G55560 46.154 91 46 1 40 130 141 228 6.91e-11 60.8

Find 58 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCGTCAGCCCGATTCTTC+TGG 0.153062 8.4:-22955819 MS.gene72105:CDS
ACATCATGCTTCTGATTTCT+TGG 0.217240 8.4:+22954978 None:intergenic
TTTGAAATTCTGTCTTTATC+TGG 0.274522 8.4:-22955592 MS.gene72105:CDS
TTCGTCAGCCCGATTCTTCT+GGG 0.316075 8.4:-22955818 MS.gene72105:CDS
GATATTAATGTGTCTCTTCC+TGG 0.316878 8.4:-22954816 MS.gene72105:CDS
CTTCCTTCCGTGGAGATAAT+TGG 0.333200 8.4:-22954881 MS.gene72105:CDS
GTTCCTCCTCCACTTTCATT+TGG 0.347793 8.4:+22955559 None:intergenic
AGGCGGCATGAGTGTGTCTT+TGG 0.382271 8.4:-22955524 MS.gene72105:CDS
CGGAGGAGTAGCTGGTTTGT+TGG 0.386323 8.4:-22955476 MS.gene72105:CDS
GAATGGGTCCCTTGCTCTGT+TGG 0.411486 8.4:-22956237 MS.gene72105:CDS
TTATCCTTTGCATCCGATGC+TGG 0.421865 8.4:+22954849 None:intergenic
GGAGGAACACGAAGCAGATC+AGG 0.444348 8.4:-22955544 MS.gene72105:CDS
GTTGTTGGCGGAGGAGTAGC+TGG 0.461135 8.4:-22955484 MS.gene72105:CDS
GTATGCATTCTTTCAGCAAA+TGG 0.463040 8.4:-22955861 MS.gene72105:CDS
AGTCCTGATGGACGTGTTGT+TGG 0.477746 8.4:-22955499 MS.gene72105:CDS
TTCTTGGCTTCTGCTCATGT+TGG 0.484881 8.4:+22954994 None:intergenic
CCGCCAACAACACGTCCATC+AGG 0.492937 8.4:+22955496 None:intergenic
GTAGGGATGGGAACAACAGC+AGG 0.496848 8.4:+22954936 None:intergenic
CACATCATCACTGTTAATGC+TGG 0.504861 8.4:-22956198 MS.gene72105:CDS
GATAGAATAAATACCTGCAC+TGG 0.507918 8.4:+22955442 None:intergenic
GAAGTTACTGATGATAGAAT+AGG 0.508118 8.4:+22954900 None:intergenic
TACTGGATCAAGAGTAGGGA+TGG 0.511973 8.4:+22954923 None:intergenic
TCCTTATGAAATTAGCCTCC+AGG 0.516288 8.4:+22954798 None:intergenic
CTTACTTAGAATTATAGTGT+TGG 0.517478 8.4:+22957325 None:intergenic
ACTGATGATAGAATAGGTAC+TGG 0.519373 8.4:+22954906 None:intergenic
TCGTCAGCCCGATTCTTCTG+GGG 0.541650 8.4:-22955817 MS.gene72105:CDS
CAGCATTAACAGTGATGATG+TGG 0.544475 8.4:+22956199 None:intergenic
AGGAGTAGCTGGTTTGTTGG+TGG 0.545177 8.4:-22955473 MS.gene72105:CDS
ATAGGTACTGGATCAAGAGT+AGG 0.547053 8.4:+22954918 None:intergenic
CCTGATGGACGTGTTGTTGG+CGG 0.547329 8.4:-22955496 MS.gene72105:CDS
GGTGGCTGCAAGTCCAGTGC+AGG 0.550883 8.4:-22955455 MS.gene72105:intron
ATGCCAAATGAAAGTGGAGG+AGG 0.551611 8.4:-22955562 MS.gene72105:CDS
GTGTCTTTGGCAAGTCCTGA+TGG 0.556787 8.4:-22955511 MS.gene72105:CDS
TCGTTCATGCCAAATGAAAG+TGG 0.559940 8.4:-22955568 MS.gene72105:CDS
AAATTTGAACCAACAGAGCA+AGG 0.567744 8.4:+22956228 None:intergenic
TAGGTACTGGATCAAGAGTA+GGG 0.580504 8.4:+22954919 None:intergenic
CGTCAGCCCGATTCTTCTGG+GGG 0.586127 8.4:-22955816 MS.gene72105:CDS
GACACATTAATATCAGCAGC+GGG 0.589557 8.4:+22954825 None:intergenic
CATCACTGTTAATGCTGGAG+AGG 0.596487 8.4:-22956193 MS.gene72105:intron
AATTTGAACCAACAGAGCAA+GGG 0.597677 8.4:+22956229 None:intergenic
TTGGTCTGCTGTGCCAGCAT+CGG 0.597910 8.4:-22954862 MS.gene72105:CDS
GATGGACGTGTTGTTGGCGG+AGG 0.605100 8.4:-22955493 MS.gene72105:CDS
TGTGCCAGCATCGGATGCAA+AGG 0.609579 8.4:-22954853 MS.gene72105:CDS
TCATCAGTAACTTCCTTCCG+TGG 0.610671 8.4:-22954891 MS.gene72105:CDS
GAAAGAATGCATACCGCTCG+AGG 0.613852 8.4:+22955870 None:intergenic
TGTATCTACCAGGTAGTGGT+TGG 0.624215 8.4:-22955035 MS.gene72105:intron
AGACCAATTATCTCCACGGA+AGG 0.635192 8.4:+22954878 None:intergenic
TTCTCAGCAAGGACCTCGAG+CGG 0.641808 8.4:-22955883 MS.gene72105:intron
AGCATTAACAGTGATGATGT+GGG 0.643782 8.4:+22956200 None:intergenic
ACTGGATCAAGAGTAGGGAT+GGG 0.644029 8.4:+22954924 None:intergenic
CAGCAGACCAATTATCTCCA+CGG 0.646524 8.4:+22954874 None:intergenic
AGACACATTAATATCAGCAG+CGG 0.659162 8.4:+22954824 None:intergenic
AACAACAGCAGGAGATGACA+CGG 0.660123 8.4:+22954947 None:intergenic
GGAACACGAAGCAGATCAGG+CGG 0.674892 8.4:-22955541 MS.gene72105:CDS
AAGAAATCAGAAGCATGATG+TGG 0.682976 8.4:-22954976 MS.gene72105:CDS
ATTAATGTGTCTCTTCCTGG+AGG 0.686956 8.4:-22954813 MS.gene72105:CDS
TTCATGCCAAATGAAAGTGG+AGG 0.721594 8.4:-22955565 MS.gene72105:CDS
GCATTAACAGTGATGATGTG+GGG 0.743775 8.4:+22956201 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTCAAATTCTTAATT+TGG - chr8.4:22955803-22955822 MS.gene72105:CDS 10.0%
!! AATTAAAAGTTAAACAAAAA+GGG + chr8.4:22955393-22955412 None:intergenic 10.0%
!!! TCTATTTTATATTTTACTAA+TGG - chr8.4:22955135-22955154 MS.gene72105:intron 10.0%
!! GAATTAAAAGTTAAACAAAA+AGG + chr8.4:22955394-22955413 None:intergenic 15.0%
!!! TGTTAACATTTACATTTTTT+AGG - chr8.4:22955895-22955914 MS.gene72105:intron 15.0%
!!! TTTGTAATTCTCTAAAAATT+TGG - chr8.4:22955499-22955518 MS.gene72105:CDS 15.0%
!! AATGAAGTGATGATAAAATT+AGG - chr8.4:22956071-22956090 MS.gene72105:intron 20.0%
!! AATTAACTCAAAACAATGTA+TGG + chr8.4:22955357-22955376 None:intergenic 20.0%
!! ACTATTTCAATATCTTAGTT+TGG - chr8.4:22955051-22955070 MS.gene72105:intron 20.0%
!! ATATGTAAAAGCAGAAATTT+GGG + chr8.4:22955456-22955475 None:intergenic 20.0%
!! ATGAAGTGATGATAAAATTA+GGG - chr8.4:22956072-22956091 MS.gene72105:intron 20.0%
!! ATTGAAAATGATAAAAGTCT+CGG + chr8.4:22955860-22955879 None:intergenic 20.0%
!!! ATTTACATTTTTTAGGTGAA+TGG - chr8.4:22955902-22955921 MS.gene72105:intron 20.0%
!!! GCATTTAGTTTCATATTAAA+TGG - chr8.4:22955591-22955610 MS.gene72105:CDS 20.0%
!!! TTGAATGAACCTTTTTAATT+TGG - chr8.4:22956801-22956820 MS.gene72105:intron 20.0%
!!! TTTACATTTTTTAGGTGAAT+GGG - chr8.4:22955903-22955922 MS.gene72105:intron 20.0%
! ATAAACAGAATAAAAGTGAG+AGG - chr8.4:22956102-22956121 MS.gene72105:intron 25.0%
! CATATGTAAAAGCAGAAATT+TGG + chr8.4:22955457-22955476 None:intergenic 25.0%
! CGTCATTCATTGAAAATTAT+AGG + chr8.4:22955239-22955258 None:intergenic 25.0%
! CTTACTTAGAATTATAGTGT+TGG + chr8.4:22954834-22954853 None:intergenic 25.0%
! TAAACAGAATAAAAGTGAGA+GGG - chr8.4:22956103-22956122 MS.gene72105:intron 25.0%
! TAAGAGTTATCTTTCTCAAA+TGG + chr8.4:22956908-22956927 None:intergenic 25.0%
! TATAGAAGAGAACTTTCATT+TGG - chr8.4:22956417-22956436 MS.gene72105:intron 25.0%
! TCTATGCTGTCTTATATATT+AGG - chr8.4:22955674-22955693 MS.gene72105:intron 25.0%
! TCTTAGAATCTTATTCTCTT+AGG - chr8.4:22955743-22955762 MS.gene72105:intron 25.0%
!! ACTCATTTGTTGAGAATTTA+TGG - chr8.4:22954933-22954952 MS.gene72105:CDS 25.0%
!! TTTGAAATTCTGTCTTTATC+TGG - chr8.4:22956564-22956583 MS.gene72105:intron 25.0%
!!! ACAAAGCTAAAGTTTTAAGT+TGG + chr8.4:22955484-22955503 None:intergenic 25.0%
!!! CTTTTTAATTCCCAAATAAC+AGG + chr8.4:22956024-22956043 None:intergenic 25.0%
!!! TAGCTTCTAAATTTGCTAAT+TGG - chr8.4:22955417-22955436 MS.gene72105:intron 25.0%
!!! TTATAAGGCGTCTATAATTT+TGG + chr8.4:22955829-22955848 None:intergenic 25.0%
ACAAAATTGCTGACAATCAT+CGG + chr8.4:22956211-22956230 None:intergenic 30.0%
AGGTATTTATTCTATCCGTA+AGG - chr8.4:22956721-22956740 MS.gene72105:intron 30.0%
ATTTCAAACATGTATCTACC+AGG - chr8.4:22957111-22957130 MS.gene72105:intron 30.0%
ATTTGCAAAATTCTCCCTTA+CGG + chr8.4:22956739-22956758 None:intergenic 30.0%
GAAAGTTCTCTTCTATAAGA+TGG + chr8.4:22956414-22956433 None:intergenic 30.0%
GAAGTTACTGATGATAGAAT+AGG + chr8.4:22957259-22957278 None:intergenic 30.0%
GAGAAAATGAATAACCTTCA+TGG + chr8.4:22956258-22956277 None:intergenic 30.0%
GAGGGTAAATATCTTATATC+AGG + chr8.4:22955630-22955649 None:intergenic 30.0%
GGTATTTATTCTATCCGTAA+GGG - chr8.4:22956722-22956741 MS.gene72105:intron 30.0%
GTAGATCATTAAATGCATTG+TGG + chr8.4:22956445-22956464 None:intergenic 30.0%
TAGTAAGAACTTACTGACAT+AGG + chr8.4:22954903-22954922 None:intergenic 30.0%
TATCTTATATCAGGTAGTCA+AGG + chr8.4:22955621-22955640 None:intergenic 30.0%
TATGCTGTAATCCTGTTATT+TGG - chr8.4:22956010-22956029 MS.gene72105:intron 30.0%
TGAATCACGCCAAATTAAAA+AGG + chr8.4:22956813-22956832 None:intergenic 30.0%
TTATAGCAACATGAATAAGC+TGG - chr8.4:22957039-22957058 MS.gene72105:intron 30.0%
TTTGTGTGTATGGTTATGTT+AGG - chr8.4:22956226-22956245 MS.gene72105:CDS 30.0%
! AAAAACTGTGTACTGAGTTT+TGG - chr8.4:22955093-22955112 MS.gene72105:intron 30.0%
! ATGCTGTAATCCTGTTATTT+GGG - chr8.4:22956011-22956030 MS.gene72105:intron 30.0%
! GGTAGATACATGTTTGAAAT+TGG + chr8.4:22957111-22957130 None:intergenic 30.0%
! TCAGTACACAGTTTTTAACA+TGG + chr8.4:22955089-22955108 None:intergenic 30.0%
! TGATATAAAGCATCTTGAGA+TGG - chr8.4:22955704-22955723 MS.gene72105:intron 30.0%
!!! ACTTCTTTTTCAGTGATAAC+TGG - chr8.4:22956173-22956192 MS.gene72105:intron 30.0%
AAATTTGAACCAACAGAGCA+AGG + chr8.4:22955931-22955950 None:intergenic 35.0%
AATTTGAACCAACAGAGCAA+GGG + chr8.4:22955930-22955949 None:intergenic 35.0%
ACATCATGCTTCTGATTTCT+TGG + chr8.4:22957181-22957200 None:intergenic 35.0%
ACTGATGATAGAATAGGTAC+TGG + chr8.4:22957253-22957272 None:intergenic 35.0%
AGACACATTAATATCAGCAG+CGG + chr8.4:22957335-22957354 None:intergenic 35.0%
ATCCCACTATATGCTCAAAA+AGG + chr8.4:22956494-22956513 None:intergenic 35.0%
ATGTTAGGATGTTACCATGA+AGG - chr8.4:22956241-22956260 MS.gene72105:CDS 35.0%
GAAATGGAGTTGTCATTGTA+AGG + chr8.4:22956990-22957009 None:intergenic 35.0%
GAATGTTTGTTCGCTAGAAA+TGG + chr8.4:22957006-22957025 None:intergenic 35.0%
GATACAATACACATCCGATA+CGG + chr8.4:22955267-22955286 None:intergenic 35.0%
GATAGAATAAATACCTGCAC+TGG + chr8.4:22956717-22956736 None:intergenic 35.0%
GATATTAATGTGTCTCTTCC+TGG - chr8.4:22957340-22957359 MS.gene72105:CDS 35.0%
GTATGCATTCTTTCAGCAAA+TGG - chr8.4:22956295-22956314 MS.gene72105:intron 35.0%
GTTTCTTGTCTGTGATTCAT+AGG - chr8.4:22955295-22955314 MS.gene72105:intron 35.0%
TCTAGCGAACAAACATTCAA+TGG - chr8.4:22957008-22957027 MS.gene72105:intron 35.0%
! AAGAAATCAGAAGCATGATG+TGG - chr8.4:22957180-22957199 MS.gene72105:intron 35.0%
! AAGATGATAATGCTCCTCTT+TGG - chr8.4:22954876-22954895 MS.gene72105:CDS 35.0%
! AGATGATAATGCTCCTCTTT+GGG - chr8.4:22954877-22954896 MS.gene72105:CDS 35.0%
! AGCATTAACAGTGATGATGT+GGG + chr8.4:22955959-22955978 None:intergenic 35.0%
! GGTTATTCATTTTCTCAGCA+AGG - chr8.4:22956262-22956281 MS.gene72105:intron 35.0%
! TGTCAGCAATTTTGTGTGTA+TGG - chr8.4:22956216-22956235 MS.gene72105:CDS 35.0%
! TTAATTTGGCGTGATTCAAG+TGG - chr8.4:22956815-22956834 MS.gene72105:intron 35.0%
!! TGCCTTTTTGAGCATATAGT+GGG - chr8.4:22956489-22956508 MS.gene72105:intron 35.0%
!! TTTTGAGCATATAGTGGGAT+TGG - chr8.4:22956494-22956513 MS.gene72105:intron 35.0%
AACATGTATCTACCAGGTAG+TGG - chr8.4:22957117-22957136 MS.gene72105:intron 40.0%
AGTGATCACACAGATATGAC+AGG + chr8.4:22956152-22956171 None:intergenic 40.0%
ATTAATGTGTCTCTTCCTGG+AGG - chr8.4:22957343-22957362 MS.gene72105:CDS 40.0%
CACATCATCACTGTTAATGC+TGG - chr8.4:22955958-22955977 MS.gene72105:intron 40.0%
GACACATTAATATCAGCAGC+GGG + chr8.4:22957334-22957353 None:intergenic 40.0%
GTCTCGGCGTTTCAATTATA+AGG + chr8.4:22955844-22955863 None:intergenic 40.0%
GTTTGAGTCTCTTAAGCATG+TGG - chr8.4:22956844-22956863 MS.gene72105:intron 40.0%
TAAAACTACCAACCACTACC+TGG + chr8.4:22957132-22957151 None:intergenic 40.0%
TCGTTCATGCCAAATGAAAG+TGG - chr8.4:22956588-22956607 MS.gene72105:intron 40.0%
TTCATGCCAAATGAAAGTGG+AGG - chr8.4:22956591-22956610 MS.gene72105:intron 40.0%
! CAGCATTAACAGTGATGATG+TGG + chr8.4:22955960-22955979 None:intergenic 40.0%
! GCATTAACAGTGATGATGTG+GGG + chr8.4:22955958-22955977 None:intergenic 40.0%
!! ACTTATCTCGGTGTTTCTAC+AGG - chr8.4:22956536-22956555 MS.gene72105:intron 40.0%
!! ATAGGTACTGGATCAAGAGT+AGG + chr8.4:22957241-22957260 None:intergenic 40.0%
!! CTTATCTCGGTGTTTCTACA+GGG - chr8.4:22956537-22956556 MS.gene72105:intron 40.0%
!! GTGCCTTTTTGAGCATATAG+TGG - chr8.4:22956488-22956507 MS.gene72105:intron 40.0%
!! TAGGTACTGGATCAAGAGTA+GGG + chr8.4:22957240-22957259 None:intergenic 40.0%
!!! GGTAGTGGTTGGTAGTTTTA+TGG - chr8.4:22957132-22957151 MS.gene72105:intron 40.0%
AACAACAGCAGGAGATGACA+CGG + chr8.4:22957212-22957231 None:intergenic 45.0%
ACTGGATCAAGAGTAGGGAT+GGG + chr8.4:22957235-22957254 None:intergenic 45.0%
AGACCAATTATCTCCACGGA+AGG + chr8.4:22957281-22957300 None:intergenic 45.0%
ATGCCAAATGAAAGTGGAGG+AGG - chr8.4:22956594-22956613 MS.gene72105:intron 45.0%
CAGCAGACCAATTATCTCCA+CGG + chr8.4:22957285-22957304 None:intergenic 45.0%
CATCACTGTTAATGCTGGAG+AGG - chr8.4:22955963-22955982 MS.gene72105:intron 45.0%
CTGAGTTGCATGCAATCTAC+CGG - chr8.4:22955000-22955019 MS.gene72105:CDS 45.0%
CTTCCTTCCGTGGAGATAAT+TGG - chr8.4:22957275-22957294 MS.gene72105:intron 45.0%
GCACGCACAGTTACTTATCT+CGG - chr8.4:22956524-22956543 MS.gene72105:intron 45.0%
GTCTCTTAAGCATGTGGTCA+TGG - chr8.4:22956850-22956869 MS.gene72105:intron 45.0%
GTTCCTCCTCCACTTTCATT+TGG + chr8.4:22956600-22956619 None:intergenic 45.0%
TACTGGATCAAGAGTAGGGA+TGG + chr8.4:22957236-22957255 None:intergenic 45.0%
TCATCAGTAACTTCCTTCCG+TGG - chr8.4:22957265-22957284 MS.gene72105:intron 45.0%
TGAATGACGAATCGCCGTAT+CGG - chr8.4:22955250-22955269 MS.gene72105:intron 45.0%
TTACTGACATAGGCCCAAAG+AGG + chr8.4:22954893-22954912 None:intergenic 45.0%
TTATCCTTTGCATCCGATGC+TGG + chr8.4:22957310-22957329 None:intergenic 45.0%
TTCTTGGCTTCTGCTCATGT+TGG + chr8.4:22957165-22957184 None:intergenic 45.0%
TTTGGCGTGATTCAAGTGGT+CGG - chr8.4:22956819-22956838 MS.gene72105:intron 45.0%
! TGTATCTACCAGGTAGTGGT+TGG - chr8.4:22957121-22957140 MS.gene72105:intron 45.0%
!!! GTGGTTGGTAGTTTTATGGC+AGG - chr8.4:22957136-22957155 MS.gene72105:intron 45.0%
AATGTGCCCCCAGAAGAATC+GGG + chr8.4:22956349-22956368 None:intergenic 50.0%
AGTGCAGCAGCAATCTGAGA+GGG + chr8.4:22955648-22955667 None:intergenic 50.0%
ATCTCCTCCTTCACCAACAC+CGG + chr8.4:22955022-22955041 None:intergenic 50.0%
CAATGTGCCCCCAGAAGAAT+CGG + chr8.4:22956350-22956369 None:intergenic 50.0%
GAAAGAATGCATACCGCTCG+AGG + chr8.4:22956289-22956308 None:intergenic 50.0%
GTGTCTTTGGCAAGTCCTGA+TGG - chr8.4:22956645-22956664 MS.gene72105:intron 50.0%
TGCATGCAATCTACCGGTGT+TGG - chr8.4:22955006-22955025 MS.gene72105:CDS 50.0%
TTCGTCAGCCCGATTCTTCT+GGG - chr8.4:22956338-22956357 MS.gene72105:intron 50.0%
! AGTCCTGATGGACGTGTTGT+TGG - chr8.4:22956657-22956676 MS.gene72105:intron 50.0%
! TGGGGGCACATTGACATATG+AGG - chr8.4:22956357-22956376 MS.gene72105:intron 50.0%
!! AGGAGTAGCTGGTTTGTTGG+TGG - chr8.4:22956683-22956702 MS.gene72105:intron 50.0%
!! CAATCTACCGGTGTTGGTGA+AGG - chr8.4:22955012-22955031 MS.gene72105:CDS 50.0%
AGGCGGCATGAGTGTGTCTT+TGG - chr8.4:22956632-22956651 MS.gene72105:intron 55.0%
CAGTGCAGCAGCAATCTGAG+AGG + chr8.4:22955649-22955668 None:intergenic 55.0%
CTTCGTCAGCCCGATTCTTC+TGG - chr8.4:22956337-22956356 MS.gene72105:intron 55.0%
GAATGGGTCCCTTGCTCTGT+TGG - chr8.4:22955919-22955938 MS.gene72105:intron 55.0%
GGAACACGAAGCAGATCAGG+CGG - chr8.4:22956615-22956634 MS.gene72105:intron 55.0%
GGAGGAACACGAAGCAGATC+AGG - chr8.4:22956612-22956631 MS.gene72105:intron 55.0%
GTAGGGATGGGAACAACAGC+AGG + chr8.4:22957223-22957242 None:intergenic 55.0%
TCGTCAGCCCGATTCTTCTG+GGG - chr8.4:22956339-22956358 MS.gene72105:intron 55.0%
TTCTCAGCAAGGACCTCGAG+CGG - chr8.4:22956273-22956292 MS.gene72105:intron 55.0%
TTGGTCTGCTGTGCCAGCAT+CGG - chr8.4:22957294-22957313 MS.gene72105:intron 55.0%
! CCTGATGGACGTGTTGTTGG+CGG - chr8.4:22956660-22956679 MS.gene72105:intron 55.0%
! TGTGCCAGCATCGGATGCAA+AGG - chr8.4:22957303-22957322 MS.gene72105:intron 55.0%
!! CGGAGGAGTAGCTGGTTTGT+TGG - chr8.4:22956680-22956699 MS.gene72105:intron 55.0%
!! TCTACCGGTGTTGGTGAAGG+AGG - chr8.4:22955015-22955034 MS.gene72105:CDS 55.0%
CCGCCAACAACACGTCCATC+AGG + chr8.4:22956663-22956682 None:intergenic 60.0%
CGTCAGCCCGATTCTTCTGG+GGG - chr8.4:22956340-22956359 MS.gene72105:intron 60.0%
GGCGTGATTCAAGTGGTCGG+TGG - chr8.4:22956822-22956841 MS.gene72105:intron 60.0%
GTTGTTGGCGGAGGAGTAGC+TGG - chr8.4:22956672-22956691 MS.gene72105:intron 60.0%
! GATGGACGTGTTGTTGGCGG+AGG - chr8.4:22956663-22956682 MS.gene72105:intron 60.0%
GGTGGCTGCAAGTCCAGTGC+AGG - chr8.4:22956701-22956720 MS.gene72105:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 22954809 22957369 22954809 ID=MS.gene72105
chr8.4 mRNA 22954809 22957369 22954809 ID=MS.gene72105.t1;Parent=MS.gene72105
chr8.4 exon 22957330 22957369 22957330 ID=MS.gene72105.t1.exon1;Parent=MS.gene72105.t1
chr8.4 CDS 22957330 22957369 22957330 ID=cds.MS.gene72105.t1;Parent=MS.gene72105.t1
chr8.4 exon 22956194 22956261 22956194 ID=MS.gene72105.t1.exon2;Parent=MS.gene72105.t1
chr8.4 CDS 22956194 22956261 22956194 ID=cds.MS.gene72105.t1;Parent=MS.gene72105.t1
chr8.4 exon 22955800 22955898 22955800 ID=MS.gene72105.t1.exon3;Parent=MS.gene72105.t1
chr8.4 CDS 22955800 22955898 22955800 ID=cds.MS.gene72105.t1;Parent=MS.gene72105.t1
chr8.4 exon 22955456 22955620 22955456 ID=MS.gene72105.t1.exon4;Parent=MS.gene72105.t1
chr8.4 CDS 22955456 22955620 22955456 ID=cds.MS.gene72105.t1;Parent=MS.gene72105.t1
chr8.4 exon 22954809 22955045 22954809 ID=MS.gene72105.t1.exon5;Parent=MS.gene72105.t1
chr8.4 CDS 22954809 22955045 22954809 ID=cds.MS.gene72105.t1;Parent=MS.gene72105.t1
Gene Sequence

>MS.gene72105

ATGTCAGAAGCAACTTCAAATTCCAACACTATAATTCTAAGTAAGTTTCTTTATGAAAATGTTTTACAAGATGATAATGCTCCTCTTTGGGCCTATGTCAGTAAGTTCTTACTATAAAGTAAAAACTCATTTGTTGAGAATTTATGGAACTGAAACTTCTGTGTGTCTTAATGTAAATGATGAGTATTTGTCTGAGTTGCATGCAATCTACCGGTGTTGGTGAAGGAGGAGATGAAATTAATACTATTTCAATATCTTAGTTTGGATTTTTCGAAGACCATGTTAAAAACTGTGTACTGAGTTTTGGAAGATAATGATTTGCAATTTCTATTTTATATTTTACTAATGGTTAAGAGCTATGTTTTTAATTTAATTTTCTTAAGTTGAAATATTATATTTATTAGTATATTTTATATATGTATCGTATCCTATAATTTTCAATGAATGACGAATCGCCGTATCGGATGTGTATTGTATCTGATACTCGTTTCTTGTCTGTGATTCATAGGTAGTATTGTTATTTTGAATCTATAATTTCTTTTTTTCCATACATTGTTTTGAGTTAATTCAAATATGATCTTCCCTTTTTGTTTAACTTTTAATTCTCGTAGCTTCTAAATTTGCTAATTGGATGAAGCTTATTTCCCAAATTTCTGCTTTTACATATGTGCACCAACTTAAAACTTTAGCTTTGTAATTCTCTAAAAATTTGGTTGATGTCTGAGTCTGACATATATGATATGATGTAGAATTTGAAACACTTATTTACAAATAATAAAATAGCATTTAGTTTCATATTAAATGGTTTTCCTTGACTACCTGATATAAGATATTTACCCTCTCAGATTGCTGCTGCACTGTACATTCTATGCTGTCTTATATATTAGGACTAAAATGATATAAAGCATCTTGAGATGGACTTAAAAGATTATTCTCTTAGAATCTTATTCTCTTAGGACAAAAGCATAGTGATGTTTCAACTTTTATTAGATCTAAATATTCAAATTCTTAATTTGGCCAAAATTATAGACGCCTTATAATTGAAACGCCGAGACTTTTATCATTTTCAATTTGATTATTAATTATTGTTAACATTTACATTTTTTAGGTGAATGGGTCCCTTGCTCTGTTGGTTCAAATTTTACACCCCACATCATCACTGTTAATGCTGGAGAGGTAATTTTCAGAAATATCTATTGACTATGCTGTAATCCTGTTATTTGGGAATTAAAAAGTCTTATTTACATGCATGTTTTTTGTAGAATGAAGTGATGATAAAATTAGGGACTAATTATAAACAGAATAAAAGTGAGAGGGAGCATTTAGTAACATGTTAATTTCCTGTCATATCTGTGTGATCACTTACTTCTTTTTCAGTGATAACTGGAAAATAGTCTAACCGATGATTGTCAGCAATTTTGTGTGTATGGTTATGTTAGGATGTTACCATGAAGGTTATTCATTTTCTCAGCAAGGACCTCGAGCGGTATGCATTCTTTCAGCAAATGGTGTCATAAAAAGTGTCACACTTCGTCAGCCCGATTCTTCTGGGGGCACATTGACATATGAGGTGAAGCATTTGTGCAAATTTTTATTATCATTCCATCTTATAGAAGAGAACTTTCATTTGGTTCCACAATGCATTTAATGATCTACACAGTTCAAGAATGTTGTTTCTCGTGCCTTTTTGAGCATATAGTGGGATTGGATTATTTGCACGCACAGTTACTTATCTCGGTGTTTCTACAGGGACTGTTTGAAATTCTGTCTTTATCTGGTTCGTTCATGCCAAATGAAAGTGGAGGAGGAACACGAAGCAGATCAGGCGGCATGAGTGTGTCTTTGGCAAGTCCTGATGGACGTGTTGTTGGCGGAGGAGTAGCTGGTTTGTTGGTGGCTGCAAGTCCAGTGCAGGTATTTATTCTATCCGTAAGGGAGAATTTTGCAAATTAAAAGTGAAATAATAAATAAAAACAAAAACATTATTTCAAATTGAATGAACCTTTTTAATTTGGCGTGATTCAAGTGGTCGGTGGTTTGAGTCTCTTAAGCATGTGGTCATGGTTCAATCTCTAACTCATTTGTATTAAAATTCACCATTTGAGAAAGATAACTCTTAACTGCGCTTCGAATTTTCGAATAAATTAGATGCAGTTGCACGCATTTCTAGAAAACAAACCTTACAATGACAACTCCATTTCTAGCGAACAAACATTCAATGGCAACTCAATTATAGCAACATGAATAAGCTGGTGATTTAGTATTTACTCGATAACAAGTTTGTTTGATTCTGATCATTCCAATTTCAAACATGTATCTACCAGGTAGTGGTTGGTAGTTTTATGGCAGGAAACCAACATGAGCAGAAGCCAAGAAATCAGAAGCATGATGTGGTATCAACCGTGTCATCTCCTGCTGTTGTTCCCATCCCTACTCTTGATCCAGTACCTATTCTATCATCAGTAACTTCCTTCCGTGGAGATAATTGGTCTGCTGTGCCAGCATCGGATGCAAAGGATAAGCCCGCTGCTGATATTAATGTGTCTCTTCCTGGAGGCTAA
Protein sequence

>MS.gene72105.t1

MSEATSNSNTIILSEWVPCSVGSNFTPHIITVNAGEQGPRAVCILSANGVIKSVTLRQPDSSGGTLTYEGLFEILSLSGSFMPNESGGGTRSRSGGMSVSLASPDGRVVGGGVAGLLVAASPVQVVVGSFMAGNQHEQKPRNQKHDVVSTVSSPAVVPIPTLDPVPILSSVTSFRGDNWSAVPASDAKDKPAADINVSLPGG