AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059402


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059402.t1 MTR_6g047880 87.069 116 15 0 1 116 22 137 2.06e-72 212
MS.gene059402.t1 MTR_6g047900 75.424 118 22 3 1 116 22 134 3.82e-56 171
MS.gene059402.t1 MTR_7g029540 62.376 101 34 2 1 100 24 121 1.31e-37 123
MS.gene059402.t1 MTR_3g463570 50.000 112 52 3 1 111 23 131 3.41e-25 92.8
MS.gene059402.t1 MTR_3g463570 50.000 112 52 3 1 111 43 151 6.15e-25 92.4
MS.gene059402.t1 MTR_3g067280 43.750 112 60 2 1 112 22 130 5.39e-23 87.0
MS.gene059402.t1 MTR_3g067500 39.823 113 64 3 1 112 1 110 2.11e-22 84.7
MS.gene059402.t1 MTR_7g056817 43.590 117 58 5 1 112 22 135 3.06e-22 85.1
MS.gene059402.t1 MTR_3g067437 41.964 112 58 3 1 109 22 129 5.75e-22 84.3
MS.gene059402.t1 MTR_7g056803 43.519 108 48 2 18 112 27 134 6.16e-22 84.3
MS.gene059402.t1 MTR_3g067270 41.964 112 62 2 1 112 22 130 6.25e-22 84.3
MS.gene059402.t1 MTR_3g467750 41.964 112 62 2 1 112 22 130 6.25e-22 84.3
MS.gene059402.t1 MTR_3g067535 45.614 114 57 4 1 112 22 132 1.93e-21 83.2
MS.gene059402.t1 MTR_3g067550 42.241 116 62 3 1 112 1 115 8.12e-20 78.6
MS.gene059402.t1 MTR_3g067555 42.241 116 62 3 1 112 1 115 8.12e-20 78.6
MS.gene059402.t1 MTR_3g467760 50.000 74 35 1 39 112 35 106 9.91e-20 78.2
MS.gene059402.t1 MTR_3g067555 42.241 116 62 3 1 112 9 123 1.07e-19 78.2
MS.gene059402.t1 MTR_3g067540 42.241 116 62 3 1 112 22 136 1.16e-19 78.6
MS.gene059402.t1 MTR_3g067555 42.241 116 62 3 1 112 22 136 1.16e-19 78.6
MS.gene059402.t1 MTR_6g017150 48.315 89 42 3 25 112 48 133 1.88e-19 78.2
MS.gene059402.t1 MTR_7g044980 40.351 114 60 5 1 113 21 127 3.03e-19 77.8
MS.gene059402.t1 MTR_6g036620 39.640 111 64 2 2 112 23 130 3.24e-18 74.7
MS.gene059402.t1 MTR_3g436100 39.423 104 50 3 25 116 51 153 5.58e-18 74.7
MS.gene059402.t1 MTR_8g022400 43.478 92 48 3 22 112 44 132 1.76e-17 73.2
MS.gene059402.t1 MTR_8g022430 41.304 92 51 2 25 115 47 136 5.98e-17 71.6
MS.gene059402.t1 MTR_3g067580 38.462 117 61 5 1 112 30 140 1.44e-16 70.9
MS.gene059402.t1 MTR_3g067430 38.136 118 64 3 1 112 22 136 1.73e-16 70.5
MS.gene059402.t1 MTR_3g067510 38.462 117 55 5 1 112 22 126 2.36e-16 69.7
MS.gene059402.t1 MTR_8g022420 42.045 88 48 2 23 109 44 129 2.56e-16 70.1
MS.gene059402.t1 MTR_3g067510 38.462 117 55 5 1 112 27 131 2.77e-16 69.7
MS.gene059402.t1 MTR_3g067570 41.667 96 50 4 1 95 1 91 3.07e-16 68.6
MS.gene059402.t1 MTR_1g019650 42.308 104 45 4 1 103 22 111 4.03e-16 68.9
MS.gene059402.t1 MTR_7g044920 38.136 118 65 4 1 113 21 135 2.20e-15 67.8
MS.gene059402.t1 MTR_6g017170 39.423 104 59 3 1 102 22 123 3.99e-15 66.6
MS.gene059402.t1 MTR_4g026590 34.746 118 70 2 1 112 23 139 1.08e-13 63.5
MS.gene059402.t1 MTR_8g461020 36.842 95 55 2 1 94 23 113 9.61e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAGTCCTAATGTTGATTA+TGG 0.176691 6.3:-42576333 MS.gene059402:CDS
AGCATCTTCAAGAAGTCTTT+TGG 0.221192 6.3:+42576246 None:intergenic
CACAACCTTCACCGCACGTT+TGG 0.331360 6.3:+42576508 None:intergenic
AGAAGCAAGCCGTTGTTATA+TGG 0.349746 6.3:-42576550 MS.gene059402:CDS
ACAACCTTCACCGCACGTTT+GGG 0.398282 6.3:+42576509 None:intergenic
ACAAGATCTTTATATGATGC+AGG 0.450360 6.3:+42576441 None:intergenic
CACAAACCATAATCAACATT+AGG 0.450638 6.3:+42576327 None:intergenic
TTTGTTTCCCCACTGATCCA+TGG 0.464352 6.3:-42576485 MS.gene059402:CDS
TTGTTTCCCCACTGATCCAT+GGG 0.469687 6.3:-42576484 MS.gene059402:CDS
AAAGATCTTGTGAAGATACC+TGG 0.482787 6.3:-42576429 MS.gene059402:CDS
ATCTTCTGCCTCAGATAAAG+AGG 0.487203 6.3:+42576299 None:intergenic
CTTCTTGAAGATGCTTGAGA+TGG 0.501142 6.3:-42576238 MS.gene059402:CDS
TTCCCCACTGATCCATGGGC+GGG 0.517994 6.3:-42576480 MS.gene059402:CDS
ACATCCCAAACGTGCGGTGA+AGG 0.531716 6.3:-42576513 MS.gene059402:CDS
TCACCCGCCCATGGATCAGT+GGG 0.539884 6.3:+42576477 None:intergenic
GATCTTTATATGATGCAGGT+TGG 0.554280 6.3:+42576445 None:intergenic
GAATGCAAAGAGAAGGGACG+TGG 0.558645 6.3:-42576375 MS.gene059402:CDS
TCATGTGGAATGCAAAGAGA+AGG 0.568615 6.3:-42576382 MS.gene059402:CDS
GTGTGTTGCCTCTTTATCTG+AGG 0.572934 6.3:-42576307 MS.gene059402:CDS
CCATAATCAACATTAGGACT+AGG 0.583229 6.3:+42576333 None:intergenic
CAATCATATTCACCCGCCCA+TGG 0.588423 6.3:+42576468 None:intergenic
TTCACCCGCCCATGGATCAG+TGG 0.592781 6.3:+42576476 None:intergenic
AAGATCTTGTGAAGATACCT+GGG 0.596931 6.3:-42576428 MS.gene059402:CDS
TTTCCCCACTGATCCATGGG+CGG 0.606049 6.3:-42576481 MS.gene059402:CDS
CATGTGGAATGCAAAGAGAA+GGG 0.628774 6.3:-42576381 MS.gene059402:CDS
AACAAACAACCATATAACAA+CGG 0.645913 6.3:+42576541 None:intergenic
AATGCAAAGAGAAGGGACGT+GGG 0.703464 6.3:-42576374 MS.gene059402:CDS
TCAGCGACATCCCAAACGTG+CGG 0.732788 6.3:-42576519 MS.gene059402:CDS
CACCCGCCCATGGATCAGTG+GGG 0.762537 6.3:+42576478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACAAACAACCATATAACAA+CGG + chr6.3:42576256-42576275 None:intergenic 25.0%
ACAAGATCTTTATATGATGC+AGG + chr6.3:42576356-42576375 None:intergenic 30.0%
CACAAACCATAATCAACATT+AGG + chr6.3:42576470-42576489 None:intergenic 30.0%
! CAAAAGTTAGTATTTTTCCC+AGG + chr6.3:42576386-42576405 None:intergenic 30.0%
AAAGATCTTGTGAAGATACC+TGG - chr6.3:42576365-42576384 MS.gene059402:CDS 35.0%
AAGATCTTGTGAAGATACCT+GGG - chr6.3:42576366-42576385 MS.gene059402:CDS 35.0%
CCATAATCAACATTAGGACT+AGG + chr6.3:42576464-42576483 None:intergenic 35.0%
GATCTTTATATGATGCAGGT+TGG + chr6.3:42576352-42576371 None:intergenic 35.0%
! AGCATCTTCAAGAAGTCTTT+TGG + chr6.3:42576551-42576570 None:intergenic 35.0%
! CCTAGTCCTAATGTTGATTA+TGG - chr6.3:42576461-42576480 MS.gene059402:CDS 35.0%
! GCATCTTCAAGAAGTCTTTT+GGG + chr6.3:42576550-42576569 None:intergenic 35.0%
! TAACTTTTGTCAGTCTCATG+TGG - chr6.3:42576397-42576416 MS.gene059402:CDS 35.0%
!!! TTTTGGGACTGTAGATTTAG+AGG + chr6.3:42576534-42576553 None:intergenic 35.0%
AGAAGCAAGCCGTTGTTATA+TGG - chr6.3:42576244-42576263 MS.gene059402:CDS 40.0%
ATCTTCTGCCTCAGATAAAG+AGG + chr6.3:42576498-42576517 None:intergenic 40.0%
CATGTGGAATGCAAAGAGAA+GGG - chr6.3:42576413-42576432 MS.gene059402:CDS 40.0%
CTTCTTGAAGATGCTTGAGA+TGG - chr6.3:42576556-42576575 MS.gene059402:CDS 40.0%
TCATGTGGAATGCAAAGAGA+AGG - chr6.3:42576412-42576431 MS.gene059402:CDS 40.0%
AATGCAAAGAGAAGGGACGT+GGG - chr6.3:42576420-42576439 MS.gene059402:CDS 45.0%
GTGTGTTGCCTCTTTATCTG+AGG - chr6.3:42576487-42576506 MS.gene059402:CDS 45.0%
TTGTTTCCCCACTGATCCAT+GGG - chr6.3:42576310-42576329 MS.gene059402:CDS 45.0%
TTTGTTTCCCCACTGATCCA+TGG - chr6.3:42576309-42576328 MS.gene059402:CDS 45.0%
ACAACCTTCACCGCACGTTT+GGG + chr6.3:42576288-42576307 None:intergenic 50.0%
CAATCATATTCACCCGCCCA+TGG + chr6.3:42576329-42576348 None:intergenic 50.0%
GAATGCAAAGAGAAGGGACG+TGG - chr6.3:42576419-42576438 MS.gene059402:CDS 50.0%
ACATCCCAAACGTGCGGTGA+AGG - chr6.3:42576281-42576300 MS.gene059402:CDS 55.0%
CACAACCTTCACCGCACGTT+TGG + chr6.3:42576289-42576308 None:intergenic 55.0%
TCAGCGACATCCCAAACGTG+CGG - chr6.3:42576275-42576294 MS.gene059402:CDS 55.0%
TTTCCCCACTGATCCATGGG+CGG - chr6.3:42576313-42576332 MS.gene059402:CDS 55.0%
TCACCCGCCCATGGATCAGT+GGG + chr6.3:42576320-42576339 None:intergenic 60.0%
TTCACCCGCCCATGGATCAG+TGG + chr6.3:42576321-42576340 None:intergenic 60.0%
TTCCCCACTGATCCATGGGC+GGG - chr6.3:42576314-42576333 MS.gene059402:CDS 60.0%
CACCCGCCCATGGATCAGTG+GGG + chr6.3:42576319-42576338 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 42576233 42576583 42576233 ID=MS.gene059402
chr6.3 mRNA 42576233 42576583 42576233 ID=MS.gene059402.t1;Parent=MS.gene059402
chr6.3 exon 42576233 42576583 42576233 ID=MS.gene059402.t1.exon1;Parent=MS.gene059402.t1
chr6.3 CDS 42576233 42576583 42576233 ID=cds.MS.gene059402.t1;Parent=MS.gene059402.t1
Gene Sequence

>MS.gene059402

ATGGAAAAAGTAGAAGCAAGCCGTTGTTATATGGTTGTTTGTTCAGCGACATCCCAAACGTGCGGTGAAGGTTGTGTTTGTTTCCCCACTGATCCATGGGCGGGTGAATATGATTGCCAACCTGCATCATATAAAGATCTTGTGAAGATACCTGGGAAAAATACTAACTTTTGTCAGTCTCATGTGGAATGCAAAGAGAAGGGACGTGGGAGCTTCTGCGCTCGTTATCCTAGTCCTAATGTTGATTATGGTTTGTGTGTTGCCTCTTTATCTGAGGCAGAAGATTTCTTCAAGATTGCCTCTAAATCTACAGTCCCAAAAGACTTCTTGAAGATGCTTGAGATGGCTTGA

Protein sequence

>MS.gene059402.t1

MEKVEASRCYMVVCSATSQTCGEGCVCFPTDPWAGEYDCQPASYKDLVKIPGKNTNFCQSHVECKEKGRGSFCARYPSPNVDYGLCVASLSEAEDFFKIASKSTVPKDFLKMLEMA