AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72180.t1 MTR_8g096990 92.220 437 29 1 1 437 1 432 0.0 825
MS.gene72180.t1 MTR_5g080960 76.765 439 95 4 1 437 1 434 0.0 680
MS.gene72180.t1 MTR_5g080890 76.538 439 96 4 1 437 1 434 0.0 679
MS.gene72180.t1 MTR_8g038250 75.621 443 75 3 1 437 1 416 0.0 677
MS.gene72180.t1 MTR_8g038280 72.311 437 120 1 1 437 1 436 0.0 658
MS.gene72180.t1 MTR_5g044770 72.540 437 114 2 1 437 1 431 0.0 648
MS.gene72180.t1 MTR_6g090290 67.735 437 139 2 1 436 1 436 0.0 585
MS.gene72180.t1 MTR_8g038310 63.387 437 135 4 1 436 1 413 0.0 551
MS.gene72180.t1 MTR_4g109760 67.277 437 105 5 1 437 1 399 0.0 539
MS.gene72180.t1 MTR_3g028120 53.207 421 184 7 25 437 24 439 1.81e-150 434
MS.gene72180.t1 MTR_6g088760 55.231 411 168 8 1 402 1 404 1.42e-146 427
MS.gene72180.t1 MTR_2g067830 53.398 412 179 8 32 436 1 406 3.22e-146 422
MS.gene72180.t1 MTR_5g038360 50.114 437 201 9 9 436 8 436 6.58e-139 405
MS.gene72180.t1 MTR_2g105300 51.834 409 162 9 32 433 1 381 6.02e-134 390
MS.gene72180.t1 MTR_0024s0040 51.196 418 185 8 30 436 1 410 4.02e-130 382
MS.gene72180.t1 MTR_5g044570 47.875 447 186 12 1 436 1 411 2.11e-127 375
MS.gene72180.t1 MTR_0036s0070 43.722 446 221 11 1 436 1 426 5.01e-118 351
MS.gene72180.t1 MTR_0036s0050 43.237 451 225 11 1 436 1 435 1.07e-113 340
MS.gene72180.t1 MTR_0036s0110 41.138 457 233 11 1 436 1 442 1.92e-107 325
MS.gene72180.t1 MTR_5g008070 41.469 422 221 10 21 435 23 425 1.64e-99 304
MS.gene72180.t1 MTR_2g059490 41.190 437 214 11 9 437 10 411 8.10e-93 287
MS.gene72180.t1 MTR_5g008150 40.189 423 208 11 22 436 27 412 3.38e-92 285
MS.gene72180.t1 MTR_2g059460 40.426 423 202 12 23 436 25 406 8.28e-89 276
MS.gene72180.t1 MTR_5g008110 37.757 437 223 12 7 436 21 415 3.28e-88 275
MS.gene72180.t1 MTR_3g097150 37.352 423 238 10 27 437 42 449 8.29e-85 267
MS.gene72180.t1 MTR_1g060750 37.592 407 212 11 40 436 2 376 1.23e-78 249
MS.gene72180.t1 MTR_5g008090 38.816 304 167 9 142 436 21 314 8.18e-60 198
MS.gene72180.t1 MTR_4g045857 40.323 310 144 10 14 316 13 288 3.60e-58 193
MS.gene72180.t1 MTR_7g117430 33.255 424 250 16 28 434 77 484 1.23e-56 194
MS.gene72180.t1 MTR_1g115210 32.407 432 256 14 21 434 73 486 1.57e-55 191
MS.gene72180.t1 MTR_1g039270 32.952 437 238 18 28 434 58 469 7.57e-54 186
MS.gene72180.t1 MTR_2g028190 36.813 364 205 13 84 434 100 451 4.81e-52 181
MS.gene72180.t1 MTR_6g088765 43.802 242 97 5 162 402 5 208 1.12e-50 171
MS.gene72180.t1 MTR_3g046300 29.437 462 275 15 11 437 16 461 2.15e-46 166
MS.gene72180.t1 MTR_1g111350 32.778 360 212 12 84 434 129 467 3.56e-46 166
MS.gene72180.t1 MTR_6g085010 33.803 355 210 11 86 434 156 491 3.68e-45 164
MS.gene72180.t1 MTR_4g007740 33.609 363 210 12 87 435 133 478 2.27e-43 159
MS.gene72180.t1 MTR_5g091130 30.993 413 255 13 47 437 152 556 2.39e-41 154
MS.gene72180.t1 MTR_2g070430 27.601 471 289 16 2 436 3 457 2.13e-38 144
MS.gene72180.t1 MTR_3g053710 31.250 368 218 12 85 434 116 466 3.33e-38 144
MS.gene72180.t1 MTR_2g055840 29.781 366 227 10 78 422 5 361 3.69e-38 142
MS.gene72180.t1 MTR_8g103880 31.105 344 212 13 87 422 117 443 8.34e-38 143
MS.gene72180.t1 MTR_3g068000 27.649 387 218 16 89 434 76 441 5.86e-31 124
MS.gene72180.t1 MTR_7g080230 28.082 438 266 16 30 435 11 431 2.97e-30 122
MS.gene72180.t1 MTR_4g008110 55.085 118 25 1 161 278 48 137 1.11e-29 112
MS.gene72180.t1 MTR_1g078240 28.982 383 220 16 85 435 68 430 5.45e-29 119
MS.gene72180.t1 MTR_3g065160 25.201 373 237 13 88 437 76 429 5.08e-28 115
MS.gene72180.t1 MTR_7g105850 28.074 488 264 23 9 436 7 467 7.67e-28 115
MS.gene72180.t1 MTR_1g078240 29.167 384 219 17 85 435 68 431 9.53e-28 115
MS.gene72180.t1 MTR_8g094590 27.764 407 230 17 64 437 52 427 3.02e-27 113
MS.gene72180.t1 MTR_4g095270 25.542 415 266 15 41 437 37 426 4.24e-27 114
MS.gene72180.t1 MTR_4g095270 25.542 415 266 15 41 437 37 426 4.61e-27 114
MS.gene72180.t1 MTR_3g053980 29.589 365 224 11 84 434 140 485 1.05e-26 112
MS.gene72180.t1 MTR_2g084550 31.675 382 211 19 85 435 76 438 1.07e-26 112
MS.gene72180.t1 MTR_5g016260 25.798 376 230 15 84 437 86 434 1.35e-26 112
MS.gene72180.t1 MTR_6g084710 26.402 428 245 18 49 434 151 550 2.16e-26 112
MS.gene72180.t1 MTR_5g016260 25.798 376 230 15 84 437 86 434 2.16e-26 112
MS.gene72180.t1 MTR_3g096930 24.938 401 249 13 64 437 62 437 1.20e-25 108
MS.gene72180.t1 MTR_4g056420 29.396 381 220 18 85 435 76 437 3.07e-25 108
MS.gene72180.t1 MTR_7g021510 27.157 394 221 17 82 435 67 434 4.31e-25 107
MS.gene72180.t1 MTR_5g077850 25.707 389 219 16 88 437 83 440 4.40e-25 107
MS.gene72180.t1 MTR_7g062360 27.865 384 225 18 89 437 62 428 5.23e-25 107
MS.gene72180.t1 MTR_1g061940 28.755 466 267 24 8 435 7 445 6.14e-25 107
MS.gene72180.t1 MTR_8g094590 28.941 387 212 17 64 422 52 403 8.23e-25 105
MS.gene72180.t1 MTR_0945s0010 41.270 126 70 3 315 437 1 125 1.38e-24 99.8
MS.gene72180.t1 MTR_7g102750 28.460 383 221 19 85 435 80 441 2.29e-24 105
MS.gene72180.t1 MTR_2g063110 24.738 477 276 19 28 434 45 508 3.10e-24 105
MS.gene72180.t1 MTR_1g078240 30.599 317 176 14 85 377 68 364 5.93e-24 103
MS.gene72180.t1 MTR_4g100990 27.885 416 220 21 86 437 74 473 7.67e-24 103
MS.gene72180.t1 MTR_1g029810 26.357 387 221 19 85 437 56 412 9.48e-24 103
MS.gene72180.t1 MTR_8g094590 31.707 287 155 11 64 338 52 309 1.02e-23 101
MS.gene72180.t1 MTR_1g029810 26.110 383 227 18 85 437 56 412 1.17e-23 103
MS.gene72180.t1 MTR_6g084710 27.647 340 199 14 49 369 151 462 1.56e-23 103
MS.gene72180.t1 MTR_4g023650 26.420 405 241 18 66 437 42 422 2.48e-23 102
MS.gene72180.t1 MTR_2g103340 42.241 116 23 1 21 136 34 105 3.28e-22 94.0
MS.gene72180.t1 MTR_2g103340 48.052 77 35 2 355 431 88 159 4.10e-12 65.1
MS.gene72180.t1 MTR_1g037630 26.761 426 275 15 25 435 26 429 3.64e-22 98.6
MS.gene72180.t1 MTR_4g023630 25.490 408 241 18 66 437 42 422 2.79e-21 95.9
MS.gene72180.t1 MTR_8g039540 26.106 452 289 17 6 435 6 434 4.17e-21 95.5
MS.gene72180.t1 MTR_5g022880 27.110 391 225 16 79 434 96 461 6.97e-21 95.5
MS.gene72180.t1 MTR_7g102750 29.755 326 181 17 85 383 80 384 4.37e-20 92.4
MS.gene72180.t1 MTR_3g014290 28.134 359 226 13 87 435 81 417 4.83e-20 92.0
MS.gene72180.t1 MTR_7g080230 28.125 352 209 15 110 435 28 361 1.23e-19 90.9
MS.gene72180.t1 MTR_5g012490 26.025 488 278 25 9 435 5 470 1.24e-19 91.3
MS.gene72180.t1 MTR_8g075090 25.984 381 219 18 87 436 113 461 3.22e-18 87.4
MS.gene72180.t1 MTR_8g075090 26.260 377 215 18 92 437 118 462 5.88e-18 86.3
MS.gene72180.t1 MTR_5g077920 27.078 373 234 14 88 436 70 428 6.15e-18 85.9
MS.gene72180.t1 MTR_5g061690 28.011 357 199 20 93 422 108 433 4.63e-17 83.6
MS.gene72180.t1 MTR_3g087780 24.027 437 261 18 28 422 27 434 7.64e-17 83.2
MS.gene72180.t1 MTR_3g006820 23.797 395 241 18 78 437 46 415 2.40e-16 80.9
MS.gene72180.t1 MTR_8g075010 25.397 378 223 18 90 437 104 452 5.25e-16 80.5
MS.gene72180.t1 MTR_8g075090 26.590 346 199 16 92 410 118 435 1.18e-15 79.3
MS.gene72180.t1 MTR_3g100500 27.208 419 255 20 40 434 45 437 3.54e-15 77.4
MS.gene72180.t1 MTR_5g080330 25.067 375 184 18 103 434 2 322 6.80e-14 73.6
MS.gene72180.t1 MTR_5g061690 27.152 302 173 17 138 422 23 294 1.10e-12 69.7
MS.gene72180.t1 MTR_1g072380 22.807 399 221 17 86 429 56 422 2.83e-11 65.5
MS.gene72180.t1 MTR_2g037710 25.455 220 131 8 224 435 12 206 4.35e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72180.t1 AT1G64830 51.208 414 194 4 26 437 24 431 8.23e-140 407
MS.gene72180.t1 AT5G33340 51.208 414 193 5 27 437 30 437 1.86e-134 394
MS.gene72180.t1 AT2G35615 44.921 443 218 8 14 435 7 444 9.99e-115 343
MS.gene72180.t1 AT1G31450 43.318 434 224 8 16 435 17 442 3.37e-105 319
MS.gene72180.t1 AT2G28010 39.593 442 209 11 3 437 4 394 2.36e-87 272
MS.gene72180.t1 AT2G28040 40.281 427 199 12 16 437 18 393 2.41e-83 261
MS.gene72180.t1 AT2G28040 41.007 417 190 12 26 437 19 384 4.10e-83 260
MS.gene72180.t1 AT2G28220 37.528 445 220 11 3 437 4 400 1.29e-77 247
MS.gene72180.t1 AT2G28030 41.192 369 186 8 74 437 48 390 2.57e-77 246
MS.gene72180.t1 AT2G03200 36.384 437 237 12 25 437 42 461 6.61e-76 244
MS.gene72180.t1 AT2G28225 40.751 373 186 9 74 437 54 400 6.29e-75 240
MS.gene72180.t1 AT4G30030 37.867 375 195 15 79 437 70 422 7.03e-57 193
MS.gene72180.t1 AT1G01300 36.066 366 201 13 84 435 139 485 9.65e-56 192
MS.gene72180.t1 AT3G61820 35.101 396 221 16 55 434 108 483 5.95e-53 184
MS.gene72180.t1 AT3G18490 31.409 433 261 16 16 434 90 500 1.10e-49 176
MS.gene72180.t1 AT3G20015 29.412 442 263 15 20 434 51 470 1.61e-47 170
MS.gene72180.t1 AT5G10760 34.419 430 241 17 23 434 58 464 6.07e-47 168
MS.gene72180.t1 AT1G25510 33.609 363 205 14 84 434 145 483 9.19e-47 168
MS.gene72180.t1 AT5G36260 32.723 382 203 15 85 435 76 434 1.28e-43 159
MS.gene72180.t1 AT2G23945 28.294 463 277 15 9 437 10 451 1.29e-43 159
MS.gene72180.t1 AT3G25700 29.630 432 249 17 39 435 39 450 4.12e-43 157
MS.gene72180.t1 AT5G10770 34.341 364 209 13 84 435 129 474 5.64e-42 155
MS.gene72180.t1 AT1G79720 31.806 371 207 13 87 434 135 482 3.08e-40 150
MS.gene72180.t1 AT3G59080 31.877 389 234 15 72 437 155 535 1.93e-39 149
MS.gene72180.t1 AT4G30040 31.778 450 246 23 9 429 10 427 2.81e-39 146
MS.gene72180.t1 AT2G42980 31.807 393 231 17 72 437 145 527 6.44e-39 147
MS.gene72180.t1 AT1G65240 30.628 382 208 16 85 435 72 427 3.33e-37 142
MS.gene72180.t1 AT3G02740 30.649 385 223 15 74 435 77 440 1.17e-36 140
MS.gene72180.t1 AT3G02740 30.376 372 220 14 87 435 10 365 4.53e-36 137
MS.gene72180.t1 AT3G12700 25.778 450 301 15 5 435 26 461 9.81e-35 134
MS.gene72180.t1 AT2G39710 29.921 381 205 19 89 429 67 425 4.16e-34 132
MS.gene72180.t1 AT3G59080 29.948 384 212 14 72 437 155 499 4.70e-30 122
MS.gene72180.t1 AT4G33490 24.878 410 238 14 68 437 35 414 4.19e-29 118
MS.gene72180.t1 AT1G66180 27.586 377 226 17 88 437 73 429 6.22e-29 118
MS.gene72180.t1 AT5G43100 26.829 369 234 15 84 437 73 420 4.41e-28 116
MS.gene72180.t1 AT5G43100 26.829 369 234 15 84 437 73 420 8.10e-28 116
MS.gene72180.t1 AT4G12920 28.571 378 233 14 67 434 37 387 8.67e-28 114
MS.gene72180.t1 AT5G37540 27.614 373 229 14 88 434 81 438 2.19e-27 114
MS.gene72180.t1 AT5G02190 26.316 380 220 16 96 434 82 442 4.04e-27 113
MS.gene72180.t1 AT3G50050 26.049 453 282 19 12 437 13 439 5.72e-27 114
MS.gene72180.t1 AT5G22850 29.973 377 224 16 85 435 79 441 7.24e-27 112
MS.gene72180.t1 AT1G09750 28.649 370 221 16 85 434 102 448 1.55e-26 111
MS.gene72180.t1 AT1G44130 25.743 404 241 14 68 437 24 402 1.60e-26 110
MS.gene72180.t1 AT3G54400 27.110 391 247 13 62 435 56 425 4.58e-26 109
MS.gene72180.t1 AT1G77480 25.526 380 234 15 85 437 65 422 2.83e-25 107
MS.gene72180.t1 AT1G77480 25.526 380 234 15 85 437 65 422 5.50e-25 107
MS.gene72180.t1 AT5G45120 26.650 409 226 16 87 434 83 478 2.06e-24 105
MS.gene72180.t1 AT4G33490 27.492 331 187 12 68 375 32 332 2.33e-24 104
MS.gene72180.t1 AT1G05840 27.632 380 228 15 85 435 78 439 7.25e-24 103
MS.gene72180.t1 AT3G52500 26.247 461 259 20 40 435 24 468 1.52e-22 100
MS.gene72180.t1 AT2G36670 28.117 377 229 16 85 435 98 458 6.18e-22 98.6
MS.gene72180.t1 AT1G49050 23.925 372 221 15 104 436 49 397 7.23e-22 97.4
MS.gene72180.t1 AT1G49050 24.372 398 236 18 80 436 197 570 8.82e-22 98.6
MS.gene72180.t1 AT2G36670 28.000 375 228 16 87 435 105 463 1.87e-21 97.1
MS.gene72180.t1 AT2G36670 28.000 375 228 16 87 435 34 392 2.12e-21 96.3
MS.gene72180.t1 AT2G36670 28.000 375 228 16 87 435 34 392 2.12e-21 96.3
MS.gene72180.t1 AT1G08210 28.382 377 227 16 85 435 116 475 3.05e-21 96.7
MS.gene72180.t1 AT1G08210 28.382 377 227 16 85 435 82 441 3.07e-21 96.3
MS.gene72180.t1 AT1G08210 28.382 377 227 16 85 435 130 489 3.47e-21 96.3
MS.gene72180.t1 AT2G17760 27.297 370 213 17 92 434 109 449 4.72e-21 95.9
MS.gene72180.t1 AT5G07030 26.293 464 289 18 2 435 15 455 6.77e-20 92.0
MS.gene72180.t1 AT1G49050 26.316 285 170 14 80 347 197 458 2.86e-17 84.3
MS.gene72180.t1 AT1G08210 26.485 404 227 16 85 435 82 468 6.47e-17 83.2
MS.gene72180.t1 AT3G42550 24.251 367 206 12 87 435 78 390 2.41e-16 81.3
MS.gene72180.t1 AT3G51350 25.469 373 231 16 87 434 102 452 3.30e-16 81.3
MS.gene72180.t1 AT3G25700 29.592 196 111 7 39 221 39 220 5.69e-16 79.3
MS.gene72180.t1 AT4G35880 24.538 379 221 18 90 437 110 454 1.28e-13 73.2
MS.gene72180.t1 AT4G16563 26.585 410 210 21 104 437 100 494 2.49e-13 72.0
MS.gene72180.t1 AT3G51330 24.932 369 229 16 92 434 107 453 9.15e-12 67.0
MS.gene72180.t1 AT3G51330 24.932 369 229 16 92 434 107 453 1.04e-11 67.0
MS.gene72180.t1 AT4G35880 24.425 348 206 16 90 410 110 427 4.78e-11 64.7

Find 72 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTAATATTTATAATAATT+TGG 0.118502 4.2:-6917440 MS.gene72180:CDS
GGAGATGCCGCTGTGGTTTC+TGG 0.147563 4.2:-6917634 MS.gene72180:CDS
TGTGAATATACTATTGCTTA+TGG 0.243798 4.2:-6917940 MS.gene72180:CDS
GACAATACAATGTCGTTTAA+AGG 0.262527 4.2:-6917811 MS.gene72180:CDS
TTAAAATGCGCTGTTATTAG+AGG 0.262980 4.2:+6917319 None:intergenic
ACAATGCCATATATCTTAAA+TGG 0.279082 4.2:+6918132 None:intergenic
GGAAACAAAAGAATAGAATT+TGG 0.288475 4.2:-6917532 MS.gene72180:CDS
TCATTTCCTAAAACTGTGAT+AGG 0.290097 4.2:-6917844 MS.gene72180:CDS
TCAGGAATTACAGTTGATTC+AGG 0.295216 4.2:+6918195 None:intergenic
AGCCCACCATTTAAGATATA+TGG 0.321843 4.2:-6918138 MS.gene72180:CDS
AAGACAAGCAAACTCAGTTT+TGG 0.327541 4.2:-6917655 MS.gene72180:CDS
TGTTAAATAGTAGAAAACTA+TGG 0.332231 4.2:+6917573 None:intergenic
GGTAACATCATAATTGATTC+AGG 0.364855 4.2:-6917484 MS.gene72180:CDS
AAATTATTATAAATATTAGA+TGG 0.365192 4.2:+6917442 None:intergenic
ATGGAAACAAAGGTACTAAT+AGG 0.366817 4.2:+6917274 None:intergenic
ATGAGATACTCACCATTATC+AGG 0.381520 4.2:+6918177 None:intergenic
CAAAGGTTTAATTGTTGACT+TGG 0.381798 4.2:+6917373 None:intergenic
CTTAGCTCAGCAGAATTTGT+TGG 0.389893 4.2:-6917191 MS.gene72180:CDS
ATACAGGTAGTGACATTGTT+TGG 0.394829 4.2:-6918107 MS.gene72180:CDS
TCAACTGTAATTCCTGATAA+TGG 0.402401 4.2:-6918189 MS.gene72180:CDS
TCTTCGGGCGTTGCTATCTT+CGG 0.415446 4.2:-6917217 MS.gene72180:CDS
TCTGGTATAGTTGGCCTTGG+AGG 0.436740 4.2:-6917781 MS.gene72180:CDS
TGGTGGGCTGCCAATTGAAT+AGG 0.439433 4.2:+6918152 None:intergenic
ACACAACTTGGATCGTCAAT+TGG 0.440174 4.2:-6917739 MS.gene72180:CDS
AGCTCTGGTATAGTTGGCCT+TGG 0.443123 4.2:-6917784 MS.gene72180:CDS
GTTAAATAGTAGAAAACTAT+GGG 0.448636 4.2:+6917574 None:intergenic
GTTTAAACTTAGGAGTGGTT+TGG 0.455654 4.2:+6918052 None:intergenic
TCGTTTAAAGGCGCAAGCTC+TGG 0.458230 4.2:-6917799 MS.gene72180:CDS
TTTAAACGACATTGTATTGT+CGG 0.466344 4.2:+6917813 None:intergenic
TATCTCATGACCTATTCAAT+TGG 0.471723 4.2:-6918162 MS.gene72180:CDS
CCTAAAACTGTGATAGGATG+TGG 0.472735 4.2:-6917838 MS.gene72180:CDS
GGTATAGTTGGCCTTGGAGG+TGG 0.473391 4.2:-6917778 MS.gene72180:CDS
AAAAGAAGCAATGAAACAAA+GGG 0.475787 4.2:+6918383 None:intergenic
GTGCGGCATTGACAACTTGT+TGG 0.485070 4.2:+6918271 None:intergenic
GGCGCAAGCTCTGGTATAGT+TGG 0.493766 4.2:-6917790 MS.gene72180:CDS
GGAATCAGCCGTGGTAGAAT+CGG 0.498390 4.2:-6917419 MS.gene72180:CDS
ATATATGGCATTGTTGATAC+AGG 0.504947 4.2:-6918123 MS.gene72180:CDS
ACCTTTGTTTCCATTGCCAA+TGG 0.512736 4.2:-6917265 MS.gene72180:CDS
GATAGCAACGCCCGAAGAAG+CGG 0.523072 4.2:+6917222 None:intergenic
GCAAACAATGCCATTGGCAA+TGG 0.526861 4.2:+6917255 None:intergenic
ATGCCATATATCTTAAATGG+TGG 0.536932 4.2:+6918135 None:intergenic
ACATTGGAAGCATTTAGTGT+TGG 0.540009 4.2:-6917553 MS.gene72180:CDS
ATCATCAAATGGTGGTGATG+AGG 0.548330 4.2:-6917506 MS.gene72180:CDS
AGCAAAGCAAACAATGCCAT+TGG 0.555164 4.2:+6917249 None:intergenic
GCTCAGCAGAATTTGTTGGT+TGG 0.563160 4.2:-6917187 MS.gene72180:CDS
CTTACATTAGAGTCCTCTAC+TGG 0.564370 4.2:-6917877 MS.gene72180:CDS
TGAAATATGACCGCCAGTAG+AGG 0.573661 4.2:+6917864 None:intergenic
TGCCATATATCTTAAATGGT+GGG 0.574669 4.2:+6918136 None:intergenic
GAATTTGGCAGATCATCAAA+TGG 0.575497 4.2:-6917517 MS.gene72180:CDS
TCATCAAATGGTGGTGATGA+GGG 0.576269 4.2:-6917505 MS.gene72180:CDS
TACACTTTGCTACAATCAGT+AGG 0.577745 4.2:+6917130 None:intergenic
TGCTATAGTACCACATCAGA+CGG 0.579957 4.2:-6917352 MS.gene72180:CDS
TGACATAGGTTAGATGAACA+TGG 0.583072 4.2:+6918000 None:intergenic
TATCTCTCACAGATTGACAT+AGG 0.588294 4.2:+6917986 None:intergenic
GCCATTGGCAATGGAAACAA+AGG 0.590979 4.2:+6917264 None:intergenic
TGATGGTTTAAACTTAGGAG+TGG 0.593505 4.2:+6918047 None:intergenic
GTGTCTCTTATAACACAACT+TGG 0.612159 4.2:-6917751 MS.gene72180:CDS
AAAATTATATCCGTCTGATG+TGG 0.617721 4.2:+6917342 None:intergenic
CTGATGTGGTACTATAGCAA+AGG 0.621790 4.2:+6917356 None:intergenic
GAGACACGGGACCACCTCCA+AGG 0.631998 4.2:+6917767 None:intergenic
GAGTTTGACTCAAAAGACAA+TGG 0.632515 4.2:+6917691 None:intergenic
GACATAGGTTAGATGAACAT+GGG 0.639950 4.2:+6918001 None:intergenic
TAATAATTTGGAATCAGCCG+TGG 0.648028 4.2:-6917428 MS.gene72180:CDS
AAGTTGTGTTATAAGAGACA+CGG 0.651825 4.2:+6917753 None:intergenic
GGAGATCATTCACATTCACA+AGG 0.667677 4.2:-6917919 MS.gene72180:CDS
AGTTGTGTTATAAGAGACAC+GGG 0.672510 4.2:+6917754 None:intergenic
ACATTAGAGTCCTCTACTGG+CGG 0.682517 4.2:-6917874 MS.gene72180:CDS
TTTGGCAGATCATCAAATGG+TGG 0.685338 4.2:-6917514 MS.gene72180:CDS
TTAAATAGTAGAAAACTATG+GGG 0.716714 4.2:+6917575 None:intergenic
ACGGTTGATAGAACGACGTG+CGG 0.717425 4.2:+6918254 None:intergenic
GAGTTTCACCGATTCTACCA+CGG 0.727068 4.2:+6917411 None:intergenic
ATCATCACCAGAAACCACAG+CGG 0.787801 4.2:+6917627 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTATATTCACAAAAATTTT+GGG + chr4.2:6917601-6917620 None:intergenic 15.0%
!!! TAGTATATTCACAAAAATTT+TGG + chr4.2:6917602-6917621 None:intergenic 15.0%
!! GTTAAATAGTAGAAAACTAT+GGG + chr4.2:6917978-6917997 None:intergenic 20.0%
!! TGTTAAATAGTAGAAAACTA+TGG + chr4.2:6917979-6917998 None:intergenic 20.0%
!! TTAAATAGTAGAAAACTATG+GGG + chr4.2:6917977-6917996 None:intergenic 20.0%
!!! AGTTTTCTACTATTTAACAT+TGG - chr4.2:6917980-6917999 MS.gene72180:CDS 20.0%
!!! TTTTGTAGAAATGATTAACA+CGG + chr4.2:6917317-6917336 None:intergenic 20.0%
! AAAAAGAAGCAATGAAACAA+AGG + chr4.2:6917170-6917189 None:intergenic 25.0%
! AAAAGAAGCAATGAAACAAA+GGG + chr4.2:6917169-6917188 None:intergenic 25.0%
! AAAGAAAAAAAGGGTTAGAA+AGG + chr4.2:6917145-6917164 None:intergenic 25.0%
! AACAAAGGGAAAAGAAAAAA+AGG + chr4.2:6917155-6917174 None:intergenic 25.0%
! ACAAAGGGAAAAGAAAAAAA+GGG + chr4.2:6917154-6917173 None:intergenic 25.0%
! ACAATGCCATATATCTTAAA+TGG + chr4.2:6917420-6917439 None:intergenic 25.0%
! GGAAACAAAAGAATAGAATT+TGG - chr4.2:6918017-6918036 MS.gene72180:CDS 25.0%
! TGTGAATATACTATTGCTTA+TGG - chr4.2:6917609-6917628 MS.gene72180:CDS 25.0%
! TTTAAACGACATTGTATTGT+CGG + chr4.2:6917739-6917758 None:intergenic 25.0%
!!! ATGACACAGTATTTTGTTTA+AGG + chr4.2:6918394-6918413 None:intergenic 25.0%
!!! GATTTTGATGGTTTAAACTT+AGG + chr4.2:6917510-6917529 None:intergenic 25.0%
!!! TTGTAAGTTGATGATTTTGA+TGG + chr4.2:6917522-6917541 None:intergenic 25.0%
AAAATTATATCCGTCTGATG+TGG + chr4.2:6918210-6918229 None:intergenic 30.0%
AAGTTGTGTTATAAGAGACA+CGG + chr4.2:6917799-6917818 None:intergenic 30.0%
ATATATGGCATTGTTGATAC+AGG - chr4.2:6917426-6917445 MS.gene72180:CDS 30.0%
ATGCCATATATCTTAAATGG+TGG + chr4.2:6917417-6917436 None:intergenic 30.0%
TATCTCATGACCTATTCAAT+TGG - chr4.2:6917387-6917406 MS.gene72180:CDS 30.0%
TCAACTGTAATTCCTGATAA+TGG - chr4.2:6917360-6917379 MS.gene72180:CDS 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr4.2:6917705-6917724 MS.gene72180:CDS 30.0%
TGCCATATATCTTAAATGGT+GGG + chr4.2:6917416-6917435 None:intergenic 30.0%
TTAAAATGCGCTGTTATTAG+AGG + chr4.2:6918233-6918252 None:intergenic 30.0%
! CAAAGGTTTAATTGTTGACT+TGG + chr4.2:6918179-6918198 None:intergenic 30.0%
! CTAATAACAGCGCATTTTAA+AGG - chr4.2:6918233-6918252 MS.gene72180:CDS 30.0%
! GACAATACAATGTCGTTTAA+AGG - chr4.2:6917738-6917757 MS.gene72180:CDS 30.0%
! GGTAACATCATAATTGATTC+AGG - chr4.2:6918065-6918084 MS.gene72180:CDS 30.0%
! TGGTTGTAACATTTTTCACA+AGG + chr4.2:6917480-6917499 None:intergenic 30.0%
! TTTTCTCATGCGCTAAATAA+TGG - chr4.2:6917186-6917205 MS.gene72180:CDS 30.0%
!! ATGGAAACAAAGGTACTAAT+AGG + chr4.2:6918278-6918297 None:intergenic 30.0%
AGTTGTGTTATAAGAGACAC+GGG + chr4.2:6917798-6917817 None:intergenic 35.0%
ATACAGGTAGTGACATTGTT+TGG - chr4.2:6917442-6917461 MS.gene72180:CDS 35.0%
ATGAGATACTCACCATTATC+AGG + chr4.2:6917375-6917394 None:intergenic 35.0%
GAATTTGGCAGATCATCAAA+TGG - chr4.2:6918032-6918051 MS.gene72180:CDS 35.0%
GACATAGGTTAGATGAACAT+GGG + chr4.2:6917551-6917570 None:intergenic 35.0%
GAGTTTGACTCAAAAGACAA+TGG + chr4.2:6917861-6917880 None:intergenic 35.0%
GTGTCTCTTATAACACAACT+TGG - chr4.2:6917798-6917817 MS.gene72180:CDS 35.0%
GTTTAAACTTAGGAGTGGTT+TGG + chr4.2:6917500-6917519 None:intergenic 35.0%
TACACTTTGCTACAATCAGT+AGG + chr4.2:6918422-6918441 None:intergenic 35.0%
TATCTCTCACAGATTGACAT+AGG + chr4.2:6917566-6917585 None:intergenic 35.0%
TGACATAGGTTAGATGAACA+TGG + chr4.2:6917552-6917571 None:intergenic 35.0%
TGATGGTTTAAACTTAGGAG+TGG + chr4.2:6917505-6917524 None:intergenic 35.0%
! AAGACAAGCAAACTCAGTTT+TGG - chr4.2:6917894-6917913 MS.gene72180:CDS 35.0%
! AGCCCACCATTTAAGATATA+TGG - chr4.2:6917411-6917430 MS.gene72180:CDS 35.0%
! TAATAATTTGGAATCAGCCG+TGG - chr4.2:6918121-6918140 MS.gene72180:CDS 35.0%
! TCAGGAATTACAGTTGATTC+AGG + chr4.2:6917357-6917376 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr4.2:6917996-6918015 MS.gene72180:CDS 35.0%
!! ATGGTGATTTTGAAGAGTCA+CGG + chr4.2:6917233-6917252 None:intergenic 35.0%
!!! ATGGGGTGTTTTTTCACTAT+AGG + chr4.2:6917960-6917979 None:intergenic 35.0%
!!! TGTTGGTACTTGTTTTGTGT+AGG + chr4.2:6917264-6917283 None:intergenic 35.0%
!!! TTTTGTGTAGGTTGGTAGAA+TGG + chr4.2:6917252-6917271 None:intergenic 35.0%
ACACAACTTGGATCGTCAAT+TGG - chr4.2:6917810-6917829 MS.gene72180:CDS 40.0%
AGCAAAGCAAACAATGCCAT+TGG + chr4.2:6918303-6918322 None:intergenic 40.0%
CCTAAAACTGTGATAGGATG+TGG - chr4.2:6917711-6917730 MS.gene72180:CDS 40.0%
CTGATGTGGTACTATAGCAA+AGG + chr4.2:6918196-6918215 None:intergenic 40.0%
CTTACATTAGAGTCCTCTAC+TGG - chr4.2:6917672-6917691 MS.gene72180:CDS 40.0%
CTTAGCTCAGCAGAATTTGT+TGG - chr4.2:6918358-6918377 MS.gene72180:CDS 40.0%
GGAGATCATTCACATTCACA+AGG - chr4.2:6917630-6917649 MS.gene72180:CDS 40.0%
TGCTATAGTACCACATCAGA+CGG - chr4.2:6918197-6918216 MS.gene72180:CDS 40.0%
TTTGGCAGATCATCAAATGG+TGG - chr4.2:6918035-6918054 MS.gene72180:CDS 40.0%
! ACCTTTGTTTCCATTGCCAA+TGG - chr4.2:6918284-6918303 MS.gene72180:CDS 40.0%
! ATCATCAAATGGTGGTGATG+AGG - chr4.2:6918043-6918062 MS.gene72180:CDS 40.0%
! CCACATCCTATCACAGTTTT+AGG + chr4.2:6917714-6917733 None:intergenic 40.0%
! TCATCAAATGGTGGTGATGA+GGG - chr4.2:6918044-6918063 MS.gene72180:CDS 40.0%
!! AACAGCGCATTTTAAAGGTG+CGG - chr4.2:6918238-6918257 MS.gene72180:CDS 40.0%
!! CTTTGCTTTTACCGCTTCTT+CGG - chr4.2:6918316-6918335 MS.gene72180:CDS 40.0%
!! TTTGCTTTTACCGCTTCTTC+GGG - chr4.2:6918317-6918336 MS.gene72180:CDS 40.0%
!!! GGTACTTGTTTTGTGTAGGT+TGG + chr4.2:6917260-6917279 None:intergenic 40.0%
ACATTAGAGTCCTCTACTGG+CGG - chr4.2:6917675-6917694 MS.gene72180:CDS 45.0%
ATCATCACCAGAAACCACAG+CGG + chr4.2:6917925-6917944 None:intergenic 45.0%
GCAAACAATGCCATTGGCAA+TGG + chr4.2:6918297-6918316 None:intergenic 45.0%
GCCATTGGCAATGGAAACAA+AGG + chr4.2:6918288-6918307 None:intergenic 45.0%
TGAAATATGACCGCCAGTAG+AGG + chr4.2:6917688-6917707 None:intergenic 45.0%
!! GAGTTTCACCGATTCTACCA+CGG + chr4.2:6918141-6918160 None:intergenic 45.0%
!! GCTCAGCAGAATTTGTTGGT+TGG - chr4.2:6918362-6918381 MS.gene72180:CDS 45.0%
!! GTGATTTTGAAGAGTCACGG+TGG + chr4.2:6917230-6917249 None:intergenic 45.0%
!! AAATTATTATAAATATTAGA+TGG + chr4.2:6918110-6918129 None:intergenic 5.0%
!! ATCTAATATTTATAATAATT+TGG - chr4.2:6918109-6918128 MS.gene72180:CDS 5.0%
GGAATCAGCCGTGGTAGAAT+CGG - chr4.2:6918130-6918149 MS.gene72180:CDS 50.0%
! AGCTCTGGTATAGTTGGCCT+TGG - chr4.2:6917765-6917784 MS.gene72180:CDS 50.0%
! TCGTTTAAAGGCGCAAGCTC+TGG - chr4.2:6917750-6917769 MS.gene72180:CDS 50.0%
! TCTTCGGGCGTTGCTATCTT+CGG - chr4.2:6918332-6918351 MS.gene72180:CDS 50.0%
!! ACGGTTGATAGAACGACGTG+CGG + chr4.2:6917298-6917317 None:intergenic 50.0%
!! GTGCGGCATTGACAACTTGT+TGG + chr4.2:6917281-6917300 None:intergenic 50.0%
!! TCTGGTATAGTTGGCCTTGG+AGG - chr4.2:6917768-6917787 MS.gene72180:CDS 50.0%
!! TGGTGGGCTGCCAATTGAAT+AGG + chr4.2:6917400-6917419 None:intergenic 50.0%
GATAGCAACGCCCGAAGAAG+CGG + chr4.2:6918330-6918349 None:intergenic 55.0%
! GGCGCAAGCTCTGGTATAGT+TGG - chr4.2:6917759-6917778 MS.gene72180:CDS 55.0%
!! GGTATAGTTGGCCTTGGAGG+TGG - chr4.2:6917771-6917790 MS.gene72180:CDS 55.0%
!!! CAGTTTTGGAGATGCCGCTG+TGG - chr4.2:6917908-6917927 MS.gene72180:CDS 55.0%
GGAGATGCCGCTGTGGTTTC+TGG - chr4.2:6917915-6917934 MS.gene72180:CDS 60.0%
GAGACACGGGACCACCTCCA+AGG + chr4.2:6917785-6917804 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 6917129 6918442 6917129 ID=MS.gene72180
chr4.2 mRNA 6917129 6918442 6917129 ID=MS.gene72180.t1;Parent=MS.gene72180
chr4.2 exon 6917129 6918442 6917129 ID=MS.gene72180.t1.exon1;Parent=MS.gene72180.t1
chr4.2 CDS 6917129 6918442 6917129 ID=cds.MS.gene72180.t1;Parent=MS.gene72180.t1
Gene Sequence

>MS.gene72180

ATGAACGCACTTTCCTTTCTAACCCTTTTTTTCTTTTCCCTTTGTTTCATTGCTTCTTTTTCTCATGCGCTAAATAATGGTTTTAGTGTTGAACTCATCCACCGTGACTCTTCAAAATCACCATTCTACCAACCTACACAAAACAAGTACCAACAAGTTGTCAATGCCGCACGTCGTTCTATCAACCGTGTTAATCATTTCTACAAAAATTCAATTACAAGTACACCTGAATCAACTGTAATTCCTGATAATGGTGAGTATCTCATGACCTATTCAATTGGCAGCCCACCATTTAAGATATATGGCATTGTTGATACAGGTAGTGACATTGTTTGGCTTCAATGCGAGCCTTGTGAAAAATGTTACAACCAAACCACTCCTAAGTTTAAACCATCAAAATCATCAACTTACAAAAATATCCCATGTTCATCTAACCTATGTCAATCTGTGAGAGATATCTCTTGTAATGCCCAAAATTTTTGTGAATATACTATTGCTTATGGAGATCATTCACATTCACAAGGAGATCTTAGTGTTGATACTCTTACATTAGAGTCCTCTACTGGCGGTCATATTTCATTTCCTAAAACTGTGATAGGATGTGGAACCGACAATACAATGTCGTTTAAAGGCGCAAGCTCTGGTATAGTTGGCCTTGGAGGTGGTCCCGTGTCTCTTATAACACAACTTGGATCGTCAATTGGTTCAAAATTCTCTTATTGTTTGCTTCCATTGTCTTTTGAGTCAAACTCAATTGAGTCAAACAAGACAAGCAAACTCAGTTTTGGAGATGCCGCTGTGGTTTCTGGTGATGATGTTGTGTCAACTCCTATAGTGAAAAAACACCCCATAGTTTTCTACTATTTAACATTGGAAGCATTTAGTGTTGGAAACAAAAGAATAGAATTTGGCAGATCATCAAATGGTGGTGATGAGGGTAACATCATAATTGATTCAGGTACAACATTGACGATATTGCCATCTAATATTTATAATAATTTGGAATCAGCCGTGGTAGAATCGGTGAAACTCGAGCGTGTTGATGATCCAAGTCAACAATTAAACCTTTGCTATAGTACCACATCAGACGGATATAATTTTCCTCTAATAACAGCGCATTTTAAAGGTGCGGATATTAAGTTGCATCCTATTAGTACCTTTGTTTCCATTGCCAATGGCATTGTTTGCTTTGCTTTTACCGCTTCTTCGGGCGTTGCTATCTTCGGAAACTTAGCTCAGCAGAATTTGTTGGTTGGTTATGACCTTAAACAAAATACTGTGTCATTTAAGCCTACTGATTGTAGCAAAGTGTAA
Protein sequence

>MS.gene72180.t1

MNALSFLTLFFFSLCFIASFSHALNNGFSVELIHRDSSKSPFYQPTQNKYQQVVNAARRSINRVNHFYKNSITSTPESTVIPDNGEYLMTYSIGSPPFKIYGIVDTGSDIVWLQCEPCEKCYNQTTPKFKPSKSSTYKNIPCSSNLCQSVRDISCNAQNFCEYTIAYGDHSHSQGDLSVDTLTLESSTGGHISFPKTVIGCGTDNTMSFKGASSGIVGLGGGPVSLITQLGSSIGSKFSYCLLPLSFESNSIESNKTSKLSFGDAAVVSGDDVVSTPIVKKHPIVFYYLTLEAFSVGNKRIEFGRSSNGGDEGNIIIDSGTTLTILPSNIYNNLESAVVESVKLERVDDPSQQLNLCYSTTSDGYNFPLITAHFKGADIKLHPISTFVSIANGIVCFAFTASSGVAIFGNLAQQNLLVGYDLKQNTVSFKPTDCSKV