AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72210.t1 MTR_5g073480 85.864 382 53 1 35 416 169 549 0.0 668
MS.gene72210.t1 MTR_5g073480 76.744 43 10 0 1 43 1 43 6.92e-15 77.0
MS.gene72210.t1 MTR_5g073460 65.116 387 123 3 28 403 151 536 5.30e-172 498
MS.gene72210.t1 MTR_5g073460 69.767 43 13 0 1 43 1 43 1.77e-13 72.8
MS.gene72210.t1 MTR_5g073450 66.207 290 95 2 119 408 194 480 5.72e-132 394
MS.gene72210.t1 MTR_3g031130 58.562 292 115 3 119 407 184 472 2.22e-104 322
MS.gene72210.t1 MTR_5g073620 50.142 353 144 4 53 403 225 547 1.68e-101 325
MS.gene72210.t1 MTR_5g073620 74.419 43 11 0 1 43 1 43 8.04e-14 73.9
MS.gene72210.t1 MTR_5g073770 42.414 290 149 5 22 304 102 380 3.30e-68 221
MS.gene72210.t1 MTR_5g074130 39.660 353 191 9 53 401 183 517 1.60e-64 220
MS.gene72210.t1 MTR_5g074130 70.000 40 12 0 4 43 5 44 6.24e-12 68.2
MS.gene72210.t1 MTR_5g073900 42.642 265 124 7 144 403 1 242 1.92e-50 174
MS.gene72210.t1 MTR_5g073920 33.592 387 213 12 22 401 158 507 3.45e-50 181
MS.gene72210.t1 MTR_5g073820 35.503 338 124 5 69 403 182 428 9.19e-50 175
MS.gene72210.t1 MTR_5g073820 85.294 34 5 0 10 43 29 62 5.82e-13 70.9
MS.gene72210.t1 MTR_8g021330 32.313 294 184 3 126 408 161 450 1.31e-48 175
MS.gene72210.t1 MTR_3g464010 36.982 338 186 9 78 403 193 515 2.48e-48 175
MS.gene72210.t1 MTR_3g464010 72.093 43 12 0 1 43 1 43 2.67e-14 75.5
MS.gene72210.t1 MTR_4g103540 33.677 291 186 4 114 401 237 523 5.80e-48 174
MS.gene72210.t1 MTR_6g033310 32.468 308 189 4 113 401 259 566 1.35e-46 171
MS.gene72210.t1 MTR_5g073960 40.157 254 124 7 57 305 188 418 2.79e-46 165
MS.gene72210.t1 MTR_5g073960 60.465 43 17 0 1 43 1 43 5.53e-11 64.3
MS.gene72210.t1 MTR_1g090620 32.967 273 171 1 129 401 208 468 2.72e-44 163
MS.gene72210.t1 MTR_1g057560 32.482 274 173 1 129 402 202 463 1.58e-43 161
MS.gene72210.t1 MTR_7g013770 27.564 312 207 3 111 403 203 514 3.80e-38 146
MS.gene72210.t1 MTR_7g026630 31.597 288 180 4 128 403 212 494 8.05e-38 145
MS.gene72210.t1 MTR_4g062330 31.304 230 157 1 111 339 155 384 2.07e-36 141
MS.gene72210.t1 MTR_3g025900 28.716 296 199 3 134 420 189 481 1.21e-35 139
MS.gene72210.t1 MTR_5g073650 34.310 239 113 4 72 310 121 315 5.79e-34 129
MS.gene72210.t1 MTR_2g096230 25.952 289 192 4 141 415 172 452 1.14e-33 133
MS.gene72210.t1 MTR_5g073810 34.310 239 113 4 72 310 223 417 4.94e-32 126
MS.gene72210.t1 MTR_5g073810 65.116 43 15 0 1 43 1 43 2.12e-12 68.9
MS.gene72210.t1 MTR_5g093530 36.992 246 140 4 158 401 205 437 6.73e-32 127
MS.gene72210.t1 MTR_5g093530 67.500 40 13 0 4 43 5 44 1.59e-11 66.6
MS.gene72210.t1 MTR_1g067480 26.761 284 192 5 126 397 166 445 1.76e-29 120
MS.gene72210.t1 MTR_1g067460 26.910 301 207 7 126 415 164 462 4.43e-28 117
MS.gene72210.t1 MTR_1g117090 25.373 335 213 4 104 401 175 509 5.01e-26 111
MS.gene72210.t1 MTR_5g073470 36.913 149 59 2 35 183 145 258 3.33e-19 87.4
MS.gene72210.t1 MTR_5g073470 72.093 43 12 0 1 43 1 43 1.70e-13 70.9
MS.gene72210.t1 MTR_5g073610 33.548 155 84 4 48 202 182 317 6.90e-18 84.7
MS.gene72210.t1 MTR_5g074450 72.093 43 12 0 1 43 1 43 1.58e-13 72.8
MS.gene72210.t1 MTR_5g074450 61.818 55 20 1 47 100 195 249 5.07e-11 64.7
MS.gene72210.t1 MTR_5g073660 85.294 34 5 0 10 43 29 62 4.10e-13 70.1
MS.gene72210.t1 MTR_5g073840 65.116 43 15 0 1 43 1 43 5.49e-12 66.2
MS.gene72210.t1 MTR_5g073860 67.500 40 13 0 4 43 18 57 1.03e-11 66.6
MS.gene72210.t1 MTR_5g073880 67.500 40 13 0 4 43 5 44 1.79e-11 64.7
MS.gene72210.t1 MTR_4g019260 46.552 58 31 0 206 263 10 67 4.22e-11 58.9
MS.gene72210.t1 MTR_5g074190 33.333 144 81 4 261 401 184 315 6.79e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72210.t1 AT5G58430 62.069 290 98 2 119 408 189 466 1.03e-120 364
MS.gene72210.t1 AT1G07000 48.973 292 135 3 119 408 171 450 8.40e-85 271
MS.gene72210.t1 AT5G03540 34.520 281 171 2 129 408 87 355 6.86e-48 171
MS.gene72210.t1 AT5G03540 34.520 281 171 2 129 408 202 470 3.04e-47 171
MS.gene72210.t1 AT5G03540 34.520 281 171 2 129 408 228 496 5.40e-47 171
MS.gene72210.t1 AT1G72470 32.281 285 181 3 126 401 178 459 3.60e-46 168
MS.gene72210.t1 AT5G50380 31.618 272 178 3 130 401 240 503 4.05e-43 160
MS.gene72210.t1 AT1G54090 31.228 285 182 3 126 401 170 449 4.02e-42 157
MS.gene72210.t1 AT5G52340 32.967 273 171 1 129 401 202 462 1.56e-41 155
MS.gene72210.t1 AT3G14090 28.873 284 191 3 132 408 179 458 8.11e-40 150
MS.gene72210.t1 AT5G13990 30.747 348 224 6 55 401 192 523 2.39e-39 150
MS.gene72210.t1 AT5G13150 32.180 289 179 3 132 408 210 493 9.21e-39 148
MS.gene72210.t1 AT1G07725 28.873 284 187 5 141 415 184 461 4.19e-37 143
MS.gene72210.t1 AT3G29400 25.839 298 200 4 123 401 194 489 4.53e-32 129
MS.gene72210.t1 AT5G59730 28.041 296 202 6 131 415 164 459 1.06e-30 124
MS.gene72210.t1 AT5G59730 28.041 296 202 6 131 415 164 459 1.17e-30 124
MS.gene72210.t1 AT2G28640 25.795 283 200 3 127 401 150 430 1.56e-30 124
MS.gene72210.t1 AT5G52350 28.205 273 184 1 129 401 156 416 1.89e-30 124
MS.gene72210.t1 AT5G61010 27.675 271 185 4 137 401 215 480 8.82e-29 119
MS.gene72210.t1 AT5G61010 27.675 271 185 4 137 401 215 480 8.82e-29 119
MS.gene72210.t1 AT2G28650 25.843 267 183 3 141 401 156 413 1.29e-27 115
MS.gene72210.t1 AT2G39380 25.899 278 192 3 134 401 183 456 2.03e-27 115
MS.gene72210.t1 AT3G09520 29.075 227 155 2 119 339 155 381 3.33e-26 111
MS.gene72210.t1 AT3G09530 29.703 202 139 1 141 339 174 375 1.89e-25 109
MS.gene72210.t1 AT3G55150 26.990 289 200 5 134 413 184 470 3.03e-25 108

Find 101 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGTTTGAAGAAGATTT+TGG 0.106248 5.2:+61950315 MS.gene72210:CDS
ATGCACTCTATCCTCAATTT+TGG 0.137625 5.2:-61950358 None:intergenic
CAGTTACAGGAGGGAATATT+TGG 0.189792 5.2:+61949738 MS.gene72210:CDS
AGTAGGGTCCTCGTAGATTT+TGG 0.216151 5.2:-61950451 None:intergenic
AAATCTTATCGGAATGCTTT+TGG 0.224265 5.2:+61949555 MS.gene72210:CDS
CCGGAAACTGCTGCCTTTGC+TGG 0.241109 5.2:-61950216 None:intergenic
AGTGAAGATGGGTTTGATTA+TGG 0.242259 5.2:+61950398 MS.gene72210:CDS
AAAAGCATTCCGATAAGATT+TGG 0.270775 5.2:-61949553 None:intergenic
CCAGCAAAGGCAGCAGTTTC+CGG 0.273611 5.2:+61950216 MS.gene72210:CDS
TGCTCATGCAAACTGTAAAA+TGG 0.274159 5.2:+61948876 MS.gene72210:CDS
GAATACCTTAGCGATGATTC+TGG 0.302254 5.2:+61949589 MS.gene72210:CDS
TCTTCCTTCAATTATCTGTT+TGG 0.319564 5.2:+61948974 MS.gene72210:CDS
GGAGCTATTAGAGAGCATTT+TGG 0.326513 5.2:+61950419 MS.gene72210:CDS
CCAACTGTGATGAATATAAA+TGG 0.327671 5.2:-61949459 None:intergenic
TATCCTTCACGGATGTTTGC+AGG 0.329769 5.2:+61949950 MS.gene72210:CDS
TCGACGTGTATATCAGTTAC+AGG 0.337038 5.2:+61949725 MS.gene72210:CDS
TTCAACACGTAATGGGTAAT+CGG 0.348059 5.2:-61950252 None:intergenic
CAAGAAAATCCAAATCTTAT+CGG 0.350480 5.2:+61949544 MS.gene72210:CDS
GTAGAAAGCTTATCAAGATT+AGG 0.355489 5.2:+61949760 MS.gene72210:CDS
CTTTCATCTTCTAAACTAGT+AGG 0.359405 5.2:-61949418 None:intergenic
ACAACAAAGCAACAACAATT+TGG 0.361181 5.2:+61950130 MS.gene72210:CDS
TAGAAAGCTTATCAAGATTA+GGG 0.361927 5.2:+61949761 MS.gene72210:CDS
AACAACAATTTGGAAGAGAT+TGG 0.368141 5.2:+61950140 MS.gene72210:CDS
TACTGATATTGGTAGTAGTA+TGG 0.368865 5.2:+61949486 MS.gene72210:CDS
CACTGCTGATTTATCCTTCA+CGG 0.374636 5.2:+61949939 MS.gene72210:CDS
TCACAAAACAGCAAAGTTGA+TGG 0.385033 5.2:+61949675 MS.gene72210:CDS
CTGCTGCCTTTGCTGGATCT+TGG 0.387509 5.2:-61950209 None:intergenic
AAGGCTTTGATCATGAAGTC+TGG 0.398018 5.2:-61949628 None:intergenic
ACAACAATTTGGAAGAGATT+GGG 0.400521 5.2:+61950141 MS.gene72210:CDS
CGAAACATGGACTTGAACTC+TGG 0.423943 5.2:-61950084 None:intergenic
CAGTTGGGCGGTACTGATAT+TGG 0.432419 5.2:+61949475 MS.gene72210:CDS
CCAAAGGTATGGAGATTTGT+GGG 0.434378 5.2:+61948908 MS.gene72210:CDS
TGAACGGAAACTTTGTGATC+GGG 0.445015 5.2:+61949897 MS.gene72210:CDS
CCATTTATATTCATCACAGT+TGG 0.451802 5.2:+61949459 MS.gene72210:CDS
AAGGTAGTTCAACACGTAAT+GGG 0.457041 5.2:-61950259 None:intergenic
AAGGCAGCAGTTTCCGGTGT+TGG 0.464676 5.2:+61950222 MS.gene72210:CDS
TTCCTCTCTATCAGTGAAGA+TGG 0.465906 5.2:+61950386 MS.gene72210:CDS
AGCCTTGCCAACTGAAACAA+TGG 0.468338 5.2:+61949645 MS.gene72210:CDS
CTCACCAAACAGATAATTGA+AGG 0.468970 5.2:-61948978 None:intergenic
GAAGGTAGTTCAACACGTAA+TGG 0.475458 5.2:-61950260 None:intergenic
CTTCACTGATAGAGAGGAAG+TGG 0.479915 5.2:-61950382 None:intergenic
CCCACAAATCTCCATACCTT+TGG 0.480790 5.2:-61948908 None:intergenic
GTCCATTGTTTCAGTTGGCA+AGG 0.483309 5.2:-61949647 None:intergenic
TGACAACATCTGAACGTCTT+CGG 0.483647 5.2:-61949800 None:intergenic
GGAAAACATAGCACTGAAGT+AGG 0.485132 5.2:-61950468 None:intergenic
CAGCAGGTACAGTGTTCAAA+AGG 0.485488 5.2:+61950501 MS.gene72210:CDS
TTGAAGAAGAGATTGAAAGA+TGG 0.488391 5.2:+61949833 MS.gene72210:CDS
CATTTATATTCATCACAGTT+GGG 0.489720 5.2:+61949460 MS.gene72210:CDS
ATCACAAAGTTTCCGTTCAG+TGG 0.495687 5.2:-61949893 None:intergenic
CTGAACGGAAACTTTGTGAT+CGG 0.497291 5.2:+61949896 MS.gene72210:CDS
TTGTCAGTCACGGATGGTGC+TGG 0.503030 5.2:+61950290 MS.gene72210:CDS
TGTACCTGCTGTAATTCATC+AGG 0.503183 5.2:-61950489 None:intergenic
TTTCATCTTCTAAACTAGTA+GGG 0.514168 5.2:-61949417 None:intergenic
ACCAAAGGTATGGAGATTTG+TGG 0.514902 5.2:+61948907 MS.gene72210:CDS
GCAAAGTTGATGGTTAGTGC+AGG 0.515007 5.2:+61949685 MS.gene72210:CDS
TCAACACGTAATGGGTAATC+GGG 0.521709 5.2:-61950251 None:intergenic
GGGTGATGTCACCAAAGGTA+TGG 0.527039 5.2:+61948897 MS.gene72210:CDS
ATGCCGTCCATTGTTTCAGT+TGG 0.529072 5.2:-61949652 None:intergenic
TCCTCTCTATCAGTGAAGAT+GGG 0.529089 5.2:+61950387 MS.gene72210:CDS
AGGCTTTGATCATGAAGTCT+GGG 0.531816 5.2:-61949627 None:intergenic
GCTCATGCAAACTGTAAAAT+GGG 0.532751 5.2:+61948877 MS.gene72210:CDS
ATCCTTCACGGATGTTTGCA+GGG 0.539926 5.2:+61949951 MS.gene72210:CDS
GAAGGAATATCCCAAAATTG+AGG 0.542073 5.2:+61950347 MS.gene72210:CDS
TAAACAACCGCTCAGGTGAT+CGG 0.543038 5.2:-61950023 None:intergenic
CAACAATTTGGAAGAGATTG+GGG 0.543080 5.2:+61950142 MS.gene72210:CDS
CAAAGTTGATGGTTAGTGCA+GGG 0.555798 5.2:+61949686 MS.gene72210:CDS
ACTGTATTGATCACGAAACA+TGG 0.560282 5.2:-61950097 None:intergenic
AGATATTATTTCCCACTGAA+CGG 0.563648 5.2:+61949881 MS.gene72210:CDS
TTTACAGTTTGCATGAGCAT+CGG 0.567219 5.2:-61948872 None:intergenic
TCACAAAGTTTCCGTTCAGT+GGG 0.570711 5.2:-61949892 None:intergenic
CGTTCAGATGTTGTCATGGA+AGG 0.571479 5.2:+61949807 MS.gene72210:CDS
TTTGCCGATGCTATTGCTAG+TGG 0.574984 5.2:+61949997 MS.gene72210:CDS
GGTTAGTGCAGGGTTTGAGA+AGG 0.577226 5.2:+61949696 MS.gene72210:CDS
ACCCATCTTCACTGATAGAG+AGG 0.584934 5.2:-61950388 None:intergenic
GAAAACATAGCACTGAAGTA+GGG 0.592856 5.2:-61950467 None:intergenic
TAAATCCAGAATCATCGCTA+AGG 0.600639 5.2:-61949594 None:intergenic
TGCTGATTGTCAGTCACGGA+TGG 0.600899 5.2:+61950284 MS.gene72210:CDS
ATGACTCTAAACAACCGCTC+AGG 0.601313 5.2:-61950030 None:intergenic
TTCCCTGCAAACATCCGTGA+AGG 0.612227 5.2:-61949953 None:intergenic
GTGACTGACAATCAGCATGA+AGG 0.619865 5.2:-61950278 None:intergenic
AAGACGTTCAGATGTTGTCA+TGG 0.622352 5.2:+61949803 MS.gene72210:CDS
TTCATGCTGATTGTCAGTCA+CGG 0.623312 5.2:+61950280 MS.gene72210:CDS
GAAGGATAAATCAGCAGTGG+AGG 0.628489 5.2:-61949935 None:intergenic
AAAATTCAGCAGCTGAAGTG+CGG 0.630978 5.2:-61949977 None:intergenic
CGTGAAGGATAAATCAGCAG+TGG 0.632684 5.2:-61949938 None:intergenic
TCAGTCACGGATGGTGCTGG+AGG 0.634051 5.2:+61950293 MS.gene72210:CDS
ACAACTTAGTGATTATGTGA+AGG 0.636749 5.2:+61949513 MS.gene72210:CDS
TGATATTGGTAGTAGTATGG+TGG 0.641647 5.2:+61949489 MS.gene72210:CDS
GCTTCCACTAGCAATAGCAT+CGG 0.641991 5.2:-61950001 None:intergenic
GATTCGCCAAGATCCAGCAA+AGG 0.649881 5.2:+61950203 MS.gene72210:CDS
AACAATTTGGAAGAGATTGG+GGG 0.658750 5.2:+61950143 MS.gene72210:CDS
ACGTGTATATCAGTTACAGG+AGG 0.665468 5.2:+61949728 MS.gene72210:CDS
CGTGTATATCAGTTACAGGA+GGG 0.666664 5.2:+61949729 MS.gene72210:CDS
AAAATGGGTGATGTCACCAA+AGG 0.672395 5.2:+61948892 MS.gene72210:CDS
TTGCCAACTGAAACAATGGA+CGG 0.672416 5.2:+61949649 MS.gene72210:CDS
TAATATCTTCAAAGCCACGT+TGG 0.673863 5.2:-61949866 None:intergenic
TTATATTCATCACAGTTGGG+CGG 0.683805 5.2:+61949463 MS.gene72210:CDS
AATCGGGTGAAGTCCAACAC+CGG 0.686811 5.2:-61950235 None:intergenic
ATGGATTAAAATTTCCAACG+TGG 0.715635 5.2:+61949852 MS.gene72210:CDS
GTGGAAGCCGATCACCTGAG+CGG 0.733440 5.2:+61950016 MS.gene72210:CDS
AGCCAAATCCAAAATCTACG+AGG 0.755525 5.2:+61950443 MS.gene72210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATTGCAGTTATTCATTGT+TGG + chr5.2:61949319-61949338 MS.gene72210:intron 25.0%
! CAAGAAAATCCAAATCTTAT+CGG + chr5.2:61949544-61949563 MS.gene72210:CDS 25.0%
! CATTTATATTCATCACAGTT+GGG + chr5.2:61949460-61949479 MS.gene72210:CDS 25.0%
! TAGAAAGCTTATCAAGATTA+GGG + chr5.2:61949761-61949780 MS.gene72210:CDS 25.0%
! TTTCATCTTCTAAACTAGTA+GGG - chr5.2:61949420-61949439 None:intergenic 25.0%
!! AAGAGTTTGAAGAAGATTTT+GGG + chr5.2:61950316-61950335 MS.gene72210:CDS 25.0%
!! ATCTAAAACAACATCTATAG+AGG - chr5.2:61949389-61949408 None:intergenic 25.0%
!!! GATCTTCTTTACTTTTTTCT+GGG + chr5.2:61949268-61949287 MS.gene72210:intron 25.0%
!!! TGATTTTGTTTGCAAAGATA+TGG + chr5.2:61949056-61949075 MS.gene72210:intron 25.0%
!!! TTCATTTTTCTTCGATAAAG+TGG + chr5.2:61949147-61949166 MS.gene72210:intron 25.0%
AACAACAATTTGGAAGAGAT+TGG + chr5.2:61950140-61950159 MS.gene72210:CDS 30.0%
ACAACAAAGCAACAACAATT+TGG + chr5.2:61950130-61950149 MS.gene72210:CDS 30.0%
ACAACAATTTGGAAGAGATT+GGG + chr5.2:61950141-61950160 MS.gene72210:CDS 30.0%
ACAACTTAGTGATTATGTGA+AGG + chr5.2:61949513-61949532 MS.gene72210:CDS 30.0%
AGATATTATTTCCCACTGAA+CGG + chr5.2:61949881-61949900 MS.gene72210:CDS 30.0%
CCAACTGTGATGAATATAAA+TGG - chr5.2:61949462-61949481 None:intergenic 30.0%
CCATTTATATTCATCACAGT+TGG + chr5.2:61949459-61949478 MS.gene72210:CDS 30.0%
CTTTCATCTTCTAAACTAGT+AGG - chr5.2:61949421-61949440 None:intergenic 30.0%
GTAGAAAGCTTATCAAGATT+AGG + chr5.2:61949760-61949779 MS.gene72210:CDS 30.0%
TCTTCCTTCAATTATCTGTT+TGG + chr5.2:61948974-61948993 MS.gene72210:CDS 30.0%
TTGAAGAAGAGATTGAAAGA+TGG + chr5.2:61949833-61949852 MS.gene72210:CDS 30.0%
! AAATCTTATCGGAATGCTTT+TGG + chr5.2:61949555-61949574 MS.gene72210:CDS 30.0%
! ATGGATTAAAATTTCCAACG+TGG + chr5.2:61949852-61949871 MS.gene72210:CDS 30.0%
! GAAGAGTTTGAAGAAGATTT+TGG + chr5.2:61950315-61950334 MS.gene72210:CDS 30.0%
! TACTGATATTGGTAGTAGTA+TGG + chr5.2:61949486-61949505 MS.gene72210:CDS 30.0%
!! AAAAGCATTCCGATAAGATT+TGG - chr5.2:61949556-61949575 None:intergenic 30.0%
!!! ATTTTGGGATATTCCTTCAA+CGG - chr5.2:61950345-61950364 None:intergenic 30.0%
!!! GGATCTTCTTTACTTTTTTC+TGG + chr5.2:61949267-61949286 MS.gene72210:intron 30.0%
!!! TTTGTGATCGGGTTTTATTT+GGG + chr5.2:61949908-61949927 MS.gene72210:CDS 30.0%
AACAATTTGGAAGAGATTGG+GGG + chr5.2:61950143-61950162 MS.gene72210:CDS 35.0%
AAGGTAGTTCAACACGTAAT+GGG - chr5.2:61950262-61950281 None:intergenic 35.0%
ACTCAATAACATGGTAGCAA+AGG - chr5.2:61949215-61949234 None:intergenic 35.0%
CAACAATTTGGAAGAGATTG+GGG + chr5.2:61950142-61950161 MS.gene72210:CDS 35.0%
CAGTTATTCATTGTTGGAGT+AGG + chr5.2:61949325-61949344 MS.gene72210:intron 35.0%
CCATGTTATTGAGTTTGTCA+CGG + chr5.2:61949221-61949240 MS.gene72210:intron 35.0%
CTCACCAAACAGATAATTGA+AGG - chr5.2:61948981-61949000 None:intergenic 35.0%
GAAGGAATATCCCAAAATTG+AGG + chr5.2:61950347-61950366 MS.gene72210:CDS 35.0%
GCTCATGCAAACTGTAAAAT+GGG + chr5.2:61948877-61948896 MS.gene72210:CDS 35.0%
TAAATCCAGAATCATCGCTA+AGG - chr5.2:61949597-61949616 None:intergenic 35.0%
TAATATCTTCAAAGCCACGT+TGG - chr5.2:61949869-61949888 None:intergenic 35.0%
TCACAAAACAGCAAAGTTGA+TGG + chr5.2:61949675-61949694 MS.gene72210:CDS 35.0%
TGCTCATGCAAACTGTAAAA+TGG + chr5.2:61948876-61948895 MS.gene72210:CDS 35.0%
TTATATTCATCACAGTTGGG+CGG + chr5.2:61949463-61949482 MS.gene72210:CDS 35.0%
TTTACAGTTTGCATGAGCAT+CGG - chr5.2:61948875-61948894 None:intergenic 35.0%
! ACTGTATTGATCACGAAACA+TGG - chr5.2:61950100-61950119 None:intergenic 35.0%
! AGTGAAGATGGGTTTGATTA+TGG + chr5.2:61950398-61950417 MS.gene72210:CDS 35.0%
! ATGCACTCTATCCTCAATTT+TGG - chr5.2:61950361-61950380 None:intergenic 35.0%
! CAAAGCTCACACAACATTTT+TGG + chr5.2:61949102-61949121 MS.gene72210:intron 35.0%
! GAAAACATAGCACTGAAGTA+GGG - chr5.2:61950470-61950489 None:intergenic 35.0%
! GGTTTTGCTTCATCTATTCT+TGG + chr5.2:61948929-61948948 MS.gene72210:CDS 35.0%
! TCAATTATCTGTTTGGTGAG+TGG + chr5.2:61948981-61949000 MS.gene72210:intron 35.0%
! TGATATTGGTAGTAGTATGG+TGG + chr5.2:61949489-61949508 MS.gene72210:CDS 35.0%
! TGCACTCTATCCTCAATTTT+GGG - chr5.2:61950360-61950379 None:intergenic 35.0%
! TTTTCCTGATGAATTACAGC+AGG + chr5.2:61950485-61950504 MS.gene72210:CDS 35.0%
!! TTCAACACGTAATGGGTAAT+CGG - chr5.2:61950255-61950274 None:intergenic 35.0%
!!! CTTTGTGATCGGGTTTTATT+TGG + chr5.2:61949907-61949926 MS.gene72210:CDS 35.0%
AAAATGGGTGATGTCACCAA+AGG + chr5.2:61948892-61948911 MS.gene72210:CDS 40.0%
AAAATTCAGCAGCTGAAGTG+CGG - chr5.2:61949980-61949999 None:intergenic 40.0%
AAGACGTTCAGATGTTGTCA+TGG + chr5.2:61949803-61949822 MS.gene72210:CDS 40.0%
ACGTGTATATCAGTTACAGG+AGG + chr5.2:61949728-61949747 MS.gene72210:CDS 40.0%
AGCCAAATCCAAAATCTACG+AGG + chr5.2:61950443-61950462 MS.gene72210:CDS 40.0%
ATCACAAAGTTTCCGTTCAG+TGG - chr5.2:61949896-61949915 None:intergenic 40.0%
CAGTTACAGGAGGGAATATT+TGG + chr5.2:61949738-61949757 MS.gene72210:CDS 40.0%
CCGTGACAAACTCAATAACA+TGG - chr5.2:61949224-61949243 None:intergenic 40.0%
CGTGTATATCAGTTACAGGA+GGG + chr5.2:61949729-61949748 MS.gene72210:CDS 40.0%
CTGAACGGAAACTTTGTGAT+CGG + chr5.2:61949896-61949915 MS.gene72210:CDS 40.0%
GAAGGTAGTTCAACACGTAA+TGG - chr5.2:61950263-61950282 None:intergenic 40.0%
GAATACCTTAGCGATGATTC+TGG + chr5.2:61949589-61949608 MS.gene72210:CDS 40.0%
TCACAAAGTTTCCGTTCAGT+GGG - chr5.2:61949895-61949914 None:intergenic 40.0%
TCCTCTCTATCAGTGAAGAT+GGG + chr5.2:61950387-61950406 MS.gene72210:CDS 40.0%
TCGACGTGTATATCAGTTAC+AGG + chr5.2:61949725-61949744 MS.gene72210:CDS 40.0%
TGAACGGAAACTTTGTGATC+GGG + chr5.2:61949897-61949916 MS.gene72210:CDS 40.0%
TGACAACATCTGAACGTCTT+CGG - chr5.2:61949803-61949822 None:intergenic 40.0%
TGTACCTGCTGTAATTCATC+AGG - chr5.2:61950492-61950511 None:intergenic 40.0%
TTCCTCTCTATCAGTGAAGA+TGG + chr5.2:61950386-61950405 MS.gene72210:CDS 40.0%
TTCTTCGATAAAGTGGTGAG+TGG + chr5.2:61949154-61949173 MS.gene72210:intron 40.0%
TTGCCAACTGAAACAATGGA+CGG + chr5.2:61949649-61949668 MS.gene72210:CDS 40.0%
! AAGGCTTTGATCATGAAGTC+TGG - chr5.2:61949631-61949650 None:intergenic 40.0%
! ACCAAAGGTATGGAGATTTG+TGG + chr5.2:61948907-61948926 MS.gene72210:CDS 40.0%
! AGGCTTTGATCATGAAGTCT+GGG - chr5.2:61949630-61949649 None:intergenic 40.0%
! CAAAGGAAGCACATGAAATG+AGG - chr5.2:61949198-61949217 None:intergenic 40.0%
! CCAAAGGTATGGAGATTTGT+GGG + chr5.2:61948908-61948927 MS.gene72210:CDS 40.0%
! GGAAAACATAGCACTGAAGT+AGG - chr5.2:61950471-61950490 None:intergenic 40.0%
! GTCCTCGTAGATTTTGGATT+TGG - chr5.2:61950448-61950467 None:intergenic 40.0%
! TTCATGCTGATTGTCAGTCA+CGG + chr5.2:61950280-61950299 MS.gene72210:CDS 40.0%
!! AGATTTTGGGTATCCGTTGA+AGG + chr5.2:61950329-61950348 MS.gene72210:CDS 40.0%
!! CAAAGTTGATGGTTAGTGCA+GGG + chr5.2:61949686-61949705 MS.gene72210:CDS 40.0%
!! CACTGCTGATTTATCCTTCA+CGG + chr5.2:61949939-61949958 MS.gene72210:CDS 40.0%
!! TCAACACGTAATGGGTAATC+GGG - chr5.2:61950254-61950273 None:intergenic 40.0%
!!! GGAGCTATTAGAGAGCATTT+TGG + chr5.2:61950419-61950438 MS.gene72210:CDS 40.0%
AACTCAGTGCGGATTTGAAG+TGG + chr5.2:61949246-61949265 MS.gene72210:intron 45.0%
ACCCATCTTCACTGATAGAG+AGG - chr5.2:61950391-61950410 None:intergenic 45.0%
AGCCTTGCCAACTGAAACAA+TGG + chr5.2:61949645-61949664 MS.gene72210:CDS 45.0%
ATCCTTCACGGATGTTTGCA+GGG + chr5.2:61949951-61949970 MS.gene72210:CDS 45.0%
ATGACTCTAAACAACCGCTC+AGG - chr5.2:61950033-61950052 None:intergenic 45.0%
ATGCCGTCCATTGTTTCAGT+TGG - chr5.2:61949655-61949674 None:intergenic 45.0%
CAGCAGGTACAGTGTTCAAA+AGG + chr5.2:61950501-61950520 MS.gene72210:CDS 45.0%
CCCACAAATCTCCATACCTT+TGG - chr5.2:61948911-61948930 None:intergenic 45.0%
CGAAACATGGACTTGAACTC+TGG - chr5.2:61950087-61950106 None:intergenic 45.0%
CGTGAAGGATAAATCAGCAG+TGG - chr5.2:61949941-61949960 None:intergenic 45.0%
CGTTCAGATGTTGTCATGGA+AGG + chr5.2:61949807-61949826 MS.gene72210:CDS 45.0%
CTTCACTGATAGAGAGGAAG+TGG - chr5.2:61950385-61950404 None:intergenic 45.0%
GAAATGAGGCTATACGCATC+TGG - chr5.2:61949184-61949203 None:intergenic 45.0%
GAAGGATAAATCAGCAGTGG+AGG - chr5.2:61949938-61949957 None:intergenic 45.0%
GCTTCCACTAGCAATAGCAT+CGG - chr5.2:61950004-61950023 None:intergenic 45.0%
GTCCATTGTTTCAGTTGGCA+AGG - chr5.2:61949650-61949669 None:intergenic 45.0%
GTGACTGACAATCAGCATGA+AGG - chr5.2:61950281-61950300 None:intergenic 45.0%
TAAACAACCGCTCAGGTGAT+CGG - chr5.2:61950026-61950045 None:intergenic 45.0%
TATCCTTCACGGATGTTTGC+AGG + chr5.2:61949950-61949969 MS.gene72210:CDS 45.0%
TTTGCCGATGCTATTGCTAG+TGG + chr5.2:61949997-61950016 MS.gene72210:CDS 45.0%
! AGTAGGGTCCTCGTAGATTT+TGG - chr5.2:61950454-61950473 None:intergenic 45.0%
!! GCAAAGTTGATGGTTAGTGC+AGG + chr5.2:61949685-61949704 MS.gene72210:CDS 45.0%
AAGAGATTGGGGGAAGCGAT+CGG + chr5.2:61950153-61950172 MS.gene72210:CDS 50.0%
AATCGGGTGAAGTCCAACAC+CGG - chr5.2:61950238-61950257 None:intergenic 50.0%
GATTCGCCAAGATCCAGCAA+AGG + chr5.2:61950203-61950222 MS.gene72210:CDS 50.0%
GGGTGATGTCACCAAAGGTA+TGG + chr5.2:61948897-61948916 MS.gene72210:CDS 50.0%
GGTTAGTGCAGGGTTTGAGA+AGG + chr5.2:61949696-61949715 MS.gene72210:CDS 50.0%
TTCCCTGCAAACATCCGTGA+AGG - chr5.2:61949956-61949975 None:intergenic 50.0%
TTGTCACGGCAAACTCAGTG+CGG + chr5.2:61949235-61949254 MS.gene72210:intron 50.0%
! TGCTGATTGTCAGTCACGGA+TGG + chr5.2:61950284-61950303 MS.gene72210:CDS 50.0%
!! CAGTTGGGCGGTACTGATAT+TGG + chr5.2:61949475-61949494 MS.gene72210:CDS 50.0%
!!! AGCGATCGGAGGCATTTTCA+TGG + chr5.2:61950167-61950186 MS.gene72210:CDS 50.0%
!!! CGGAGGCATTTTCATGGAGT+TGG + chr5.2:61950173-61950192 MS.gene72210:CDS 50.0%
AAGGCAGCAGTTTCCGGTGT+TGG + chr5.2:61950222-61950241 MS.gene72210:CDS 55.0%
CCAGCAAAGGCAGCAGTTTC+CGG + chr5.2:61950216-61950235 MS.gene72210:CDS 55.0%
CTGCTGCCTTTGCTGGATCT+TGG - chr5.2:61950212-61950231 None:intergenic 55.0%
TTGTCAGTCACGGATGGTGC+TGG + chr5.2:61950290-61950309 MS.gene72210:CDS 55.0%
AGATTGGGGGAAGCGATCGG+AGG + chr5.2:61950156-61950175 MS.gene72210:CDS 60.0%
CCGGAAACTGCTGCCTTTGC+TGG - chr5.2:61950219-61950238 None:intergenic 60.0%
GTGGAAGCCGATCACCTGAG+CGG + chr5.2:61950016-61950035 MS.gene72210:CDS 60.0%
! TCAGTCACGGATGGTGCTGG+AGG + chr5.2:61950293-61950312 MS.gene72210:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 61948866 61950527 61948866 ID=MS.gene72210
chr5.2 mRNA 61948866 61950527 61948866 ID=MS.gene72210.t1;Parent=MS.gene72210
chr5.2 exon 61948866 61948995 61948866 ID=MS.gene72210.t1.exon1;Parent=MS.gene72210.t1
chr5.2 CDS 61948866 61948995 61948866 ID=cds.MS.gene72210.t1;Parent=MS.gene72210.t1
chr5.2 exon 61949395 61950527 61949395 ID=MS.gene72210.t1.exon2;Parent=MS.gene72210.t1
chr5.2 CDS 61949395 61950527 61949395 ID=cds.MS.gene72210.t1;Parent=MS.gene72210.t1
Gene Sequence

>MS.gene72210

ATGGAACCGATGCTCATGCAAACTGTAAAATGGGTGATGTCACCAAAGGTATGGAGATTTGTGGGTTTTGCTTCATCTATTCTTGGACTACTTTGTTACGCTCTCAGTTCTTCCTTCAATTATCTGTTTGGTGAGTGGAATCTGTTGAAAATCTTTCTTTACAGTGTTTTTAGTTTCATCATTTGTCTAATGATTTTGTTTGCAAAGATATGGCAACAATCAAGAAGTCTTCGATTCAAAGCTCACACAACATTTTTGGTATTGACAATCACATCACTATATTCATTTTTCTTCGATAAAGTGGTGAGTGGAAAACCAGATGCGTATAGCCTCATTTCATGTGCTTCCTTTGCTACCATGTTATTGAGTTTGTCACGGCAAACTCAGTGCGGATTTGAAGTGGATCTTCTTTACTTTTTTCTGGGATGTTTAATTGTGCAACTCATGAAGATTAAATTGCAGTTATTCATTGTTGGAGTAGGTTTTAGCTATTTAATTATTATTCTTCGTTCTTCTTTCACCTCTATAGATGTTGTTTTAGATAATGAACACCCTACTAGTTTAGAAGATGAAAGTCAGATCGTTATTGAAGTCCATTTATATTCATCACAGTTGGGCGGTACTGATATTGGTAGTAGTATGGTGGAACAACTTAGTGATTATGTGAAGGCGCTTCAGCAAGAAAATCCAAATCTTATCGGAATGCTTTTGGAGACGTTGAAAGAATACCTTAGCGATGATTCTGGATTTAATGTTTCAGACCCAGACTTCATGATCAAAGCCTTGCCAACTGAAACAATGGACGGCATTCACAAAACAGCAAAGTTGATGGTTAGTGCAGGGTTTGAGAAGGACTTCTTCGACGTGTATATCAGTTACAGGAGGGAATATTTGGTAGAAAGCTTATCAAGATTAGGGCTCAAGAAACTTAGCACCGAAGACGTTCAGATGTTGTCATGGAAGGAACTTGAAGAAGAGATTGAAAGATGGATTAAAATTTCCAACGTGGCTTTGAAGATATTATTTCCCACTGAACGGAAACTTTGTGATCGGGTTTTATTTGGGTTCTCCTCCACTGCTGATTTATCCTTCACGGATGTTTGCAGGGAATCCGCACTTCAGCTGCTGAATTTTGCCGATGCTATTGCTAGTGGAAGCCGATCACCTGAGCGGTTGTTTAGAGTCATTGACATGTTTGAAACAATGTGTGATATAATTCCAGAGTTCAAGTCCATGTTTCGTGATCAATACAGTCAGTCGCTGCACAACAAAGCAACAACAATTTGGAAGAGATTGGGGGAAGCGATCGGAGGCATTTTCATGGAGTTGGCGAATTTGATTCGCCAAGATCCAGCAAAGGCAGCAGTTTCCGGTGTTGGACTTCACCCGATTACCCATTACGTGTTGAACTACCTTCATGCTGATTGTCAGTCACGGATGGTGCTGGAGGAAGAGTTTGAAGAAGATTTTGGGTATCCGTTGAAGGAATATCCCAAAATTGAGGATAGAGTGCATTCCACTTCCTCTCTATCAGTGAAGATGGGTTTGATTATGGAGCTATTAGAGAGCATTTTGGAAGCCAAATCCAAAATCTACGAGGACCCTACTTCAGTGCTATGTTTTCCTGATGAATTACAGCAGGTACAGTGTTCAAAAGGCTGA
Protein sequence

>MS.gene72210.t1

MEPMLMQTVKWVMSPKVWRFVGFASSILGLLCYALSSSFNYLFDVVLDNEHPTSLEDESQIVIEVHLYSSQLGGTDIGSSMVEQLSDYVKALQQENPNLIGMLLETLKEYLSDDSGFNVSDPDFMIKALPTETMDGIHKTAKLMVSAGFEKDFFDVYISYRREYLVESLSRLGLKKLSTEDVQMLSWKELEEEIERWIKISNVALKILFPTERKLCDRVLFGFSSTADLSFTDVCRESALQLLNFADAIASGSRSPERLFRVIDMFETMCDIIPEFKSMFRDQYSQSLHNKATTIWKRLGEAIGGIFMELANLIRQDPAKAAVSGVGLHPITHYVLNYLHADCQSRMVLEEEFEEDFGYPLKEYPKIEDRVHSTSSLSVKMGLIMELLESILEAKSKIYEDPTSVLCFPDELQQVQCSKG