Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060258.t1 | XP_024638920.1 | 64.9 | 553 | 57 | 1 | 1 | 416 | 1 | 553 | 2.00E-184 | 655.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060258.t1 | Q9FGH9 | 62.1 | 290 | 98 | 2 | 115 | 404 | 189 | 466 | 1.5e-95 | 351.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060258.t1 | G7JZR8 | 64.3 | 549 | 59 | 1 | 1 | 412 | 1 | 549 | 5.7e-181 | 643.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene060258 | 0.8009 | 1.16E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene060258 | 0.804718 | 1.89E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene060258 | 0.817671 | 2.92E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene060258 | 0.829471 | 5.05E-55 | -1.69E-46 |
| MS.gene060257 | MS.gene060258 | 0.808156 | 3.56E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene06319 | 0.800187 | 1.62E-48 | -1.69E-46 |
| MS.gene060258 | MS.gene064321 | 0.803492 | 3.40E-49 | -1.69E-46 |
| MS.gene060258 | MS.gene065039 | 0.806338 | 8.63E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene066513 | 0.800168 | 1.64E-48 | -1.69E-46 |
| MS.gene060258 | MS.gene067087 | 0.818634 | 1.77E-52 | -1.69E-46 |
| MS.gene060258 | MS.gene070301 | 0.833881 | 4.13E-56 | -1.69E-46 |
| MS.gene060258 | MS.gene071342 | 0.806487 | 8.03E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene072280 | 0.806387 | 8.43E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene07815 | 0.806457 | 8.15E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene09233 | 0.81148 | 6.85E-51 | -1.69E-46 |
| MS.gene060258 | MS.gene26647 | 0.802585 | 5.23E-49 | -1.69E-46 |
| MS.gene060258 | MS.gene29036 | 0.80893 | 2.43E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene29096 | 0.803299 | 3.73E-49 | -1.69E-46 |
| MS.gene060258 | MS.gene32184 | 0.815243 | 1.02E-51 | -1.69E-46 |
| MS.gene060258 | MS.gene33650 | 0.813509 | 2.47E-51 | -1.69E-46 |
| MS.gene060258 | MS.gene33651 | 0.821665 | 3.58E-53 | -1.69E-46 |
| MS.gene060258 | MS.gene37029 | 0.80456 | 2.04E-49 | -1.69E-46 |
| MS.gene060258 | MS.gene38759 | 0.810954 | 8.91E-51 | -1.69E-46 |
| MS.gene060258 | MS.gene44058 | 0.81174 | 6.02E-51 | -1.69E-46 |
| MS.gene060258 | MS.gene53827 | 0.826901 | 2.10E-54 | -1.69E-46 |
| MS.gene060258 | MS.gene58653 | 0.82555 | 4.41E-54 | -1.69E-46 |
| MS.gene060258 | MS.gene63988 | 0.803022 | 4.25E-49 | -1.69E-46 |
| MS.gene060258 | MS.gene67268 | 0.864029 | 1.63E-64 | -1.69E-46 |
| MS.gene060258 | MS.gene68198 | 0.803118 | 4.06E-49 | -1.69E-46 |
| MS.gene060258 | MS.gene70656 | 0.81007 | 1.38E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene71534 | 0.804603 | 2.00E-49 | -1.69E-46 |
| MS.gene060258 | MS.gene71945 | 0.800377 | 1.48E-48 | -1.69E-46 |
| MS.gene060258 | MS.gene72210 | 0.896607 | 3.14E-76 | -1.69E-46 |
| MS.gene060258 | MS.gene74758 | 0.816239 | 6.13E-52 | -1.69E-46 |
| MS.gene060258 | MS.gene75521 | 0.814004 | 1.92E-51 | -1.69E-46 |
| MS.gene060258 | MS.gene76244 | 0.835658 | 1.48E-56 | -1.69E-46 |
| MS.gene060258 | MS.gene76755 | 0.810542 | 1.09E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene80844 | 0.83561 | 1.52E-56 | -1.69E-46 |
| MS.gene060258 | MS.gene81047 | 0.809236 | 2.09E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene81783 | 0.815391 | 9.47E-52 | -1.69E-46 |
| MS.gene060258 | MS.gene95778 | 0.810525 | 1.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060258.t1 | MTR_5g073480 | 83.163 | 392 | 66 | 0 | 21 | 412 | 158 | 549 | 0.0 | 664 |
| MS.gene060258.t1 | MTR_5g073480 | 71.111 | 45 | 13 | 0 | 1 | 45 | 1 | 45 | 1.13e-13 | 73.2 |
| MS.gene060258.t1 | MTR_5g073460 | 64.858 | 387 | 120 | 3 | 28 | 399 | 151 | 536 | 2.71e-171 | 496 |
| MS.gene060258.t1 | MTR_5g073460 | 67.442 | 43 | 14 | 0 | 1 | 43 | 1 | 43 | 3.18e-12 | 68.9 |
| MS.gene060258.t1 | MTR_5g073450 | 65.862 | 290 | 96 | 2 | 115 | 404 | 194 | 480 | 2.13e-131 | 392 |
| MS.gene060258.t1 | MTR_3g031130 | 58.562 | 292 | 115 | 3 | 115 | 403 | 184 | 472 | 3.65e-104 | 322 |
| MS.gene060258.t1 | MTR_5g073620 | 49.858 | 353 | 145 | 4 | 49 | 399 | 225 | 547 | 7.01e-101 | 323 |
| MS.gene060258.t1 | MTR_5g073620 | 72.093 | 43 | 12 | 0 | 1 | 43 | 1 | 43 | 1.55e-12 | 70.1 |
| MS.gene060258.t1 | MTR_5g073770 | 44.318 | 264 | 135 | 3 | 44 | 300 | 122 | 380 | 1.90e-68 | 222 |
| MS.gene060258.t1 | MTR_5g074130 | 39.943 | 353 | 190 | 10 | 49 | 397 | 183 | 517 | 1.30e-64 | 220 |
| MS.gene060258.t1 | MTR_5g074130 | 66.667 | 42 | 14 | 0 | 4 | 45 | 5 | 46 | 2.77e-11 | 65.9 |
| MS.gene060258.t1 | MTR_5g073920 | 33.943 | 383 | 213 | 12 | 22 | 397 | 158 | 507 | 1.83e-51 | 184 |
| MS.gene060258.t1 | MTR_5g073900 | 42.642 | 265 | 124 | 7 | 140 | 399 | 1 | 242 | 1.24e-50 | 174 |
| MS.gene060258.t1 | MTR_5g073820 | 35.503 | 338 | 124 | 5 | 65 | 399 | 182 | 428 | 9.06e-50 | 175 |
| MS.gene060258.t1 | MTR_5g073820 | 82.353 | 34 | 6 | 0 | 10 | 43 | 29 | 62 | 1.04e-11 | 67.0 |
| MS.gene060258.t1 | MTR_3g464010 | 36.982 | 338 | 186 | 9 | 74 | 399 | 193 | 515 | 2.08e-48 | 175 |
| MS.gene060258.t1 | MTR_3g464010 | 69.767 | 43 | 13 | 0 | 1 | 43 | 1 | 43 | 4.35e-13 | 71.6 |
| MS.gene060258.t1 | MTR_4g103540 | 33.677 | 291 | 186 | 4 | 110 | 397 | 237 | 523 | 3.01e-48 | 175 |
| MS.gene060258.t1 | MTR_8g021330 | 31.973 | 294 | 185 | 3 | 122 | 404 | 161 | 450 | 3.87e-48 | 173 |
| MS.gene060258.t1 | MTR_6g033310 | 32.468 | 308 | 189 | 4 | 109 | 397 | 259 | 566 | 1.69e-46 | 170 |
| MS.gene060258.t1 | MTR_5g073960 | 39.560 | 273 | 136 | 8 | 35 | 301 | 169 | 418 | 2.06e-46 | 165 |
| MS.gene060258.t1 | MTR_1g090620 | 32.967 | 273 | 171 | 1 | 125 | 397 | 208 | 468 | 3.77e-44 | 163 |
| MS.gene060258.t1 | MTR_1g057560 | 32.482 | 274 | 173 | 1 | 125 | 398 | 202 | 463 | 3.50e-43 | 160 |
| MS.gene060258.t1 | MTR_7g013770 | 27.564 | 312 | 207 | 3 | 107 | 399 | 203 | 514 | 6.13e-38 | 145 |
| MS.gene060258.t1 | MTR_7g026630 | 31.250 | 288 | 181 | 4 | 124 | 399 | 212 | 494 | 2.26e-37 | 144 |
| MS.gene060258.t1 | MTR_4g062330 | 31.304 | 230 | 157 | 1 | 107 | 335 | 155 | 384 | 3.33e-36 | 140 |
| MS.gene060258.t1 | MTR_3g025900 | 28.620 | 297 | 200 | 3 | 129 | 416 | 188 | 481 | 9.14e-36 | 139 |
| MS.gene060258.t1 | MTR_5g073650 | 34.137 | 249 | 117 | 5 | 61 | 306 | 111 | 315 | 4.72e-34 | 129 |
| MS.gene060258.t1 | MTR_2g096230 | 25.952 | 289 | 192 | 4 | 137 | 411 | 172 | 452 | 8.97e-34 | 133 |
| MS.gene060258.t1 | MTR_5g073810 | 34.137 | 249 | 117 | 5 | 61 | 306 | 213 | 417 | 5.47e-32 | 126 |
| MS.gene060258.t1 | MTR_5g073810 | 60.000 | 45 | 18 | 0 | 1 | 45 | 1 | 45 | 3.60e-11 | 65.1 |
| MS.gene060258.t1 | MTR_5g093530 | 36.992 | 246 | 140 | 5 | 154 | 397 | 205 | 437 | 1.26e-31 | 127 |
| MS.gene060258.t1 | MTR_5g093530 | 64.286 | 42 | 15 | 0 | 4 | 45 | 5 | 46 | 5.58e-11 | 64.7 |
| MS.gene060258.t1 | MTR_1g067480 | 26.761 | 284 | 192 | 5 | 122 | 393 | 166 | 445 | 2.11e-29 | 120 |
| MS.gene060258.t1 | MTR_1g067460 | 26.910 | 301 | 207 | 7 | 122 | 411 | 164 | 462 | 7.52e-28 | 116 |
| MS.gene060258.t1 | MTR_1g117090 | 27.843 | 255 | 179 | 2 | 100 | 349 | 175 | 429 | 1.17e-25 | 109 |
| MS.gene060258.t1 | MTR_5g073610 | 33.548 | 155 | 84 | 4 | 44 | 198 | 182 | 317 | 1.05e-17 | 84.0 |
| MS.gene060258.t1 | MTR_5g073470 | 35.135 | 148 | 59 | 2 | 35 | 179 | 145 | 258 | 9.90e-17 | 80.1 |
| MS.gene060258.t1 | MTR_5g073470 | 66.667 | 45 | 15 | 0 | 1 | 45 | 1 | 45 | 2.59e-12 | 67.4 |
| MS.gene060258.t1 | MTR_5g074450 | 66.667 | 45 | 15 | 0 | 1 | 45 | 1 | 45 | 2.48e-12 | 68.9 |
| MS.gene060258.t1 | MTR_5g074450 | 64.815 | 54 | 18 | 1 | 44 | 96 | 196 | 249 | 9.51e-12 | 67.0 |
| MS.gene060258.t1 | MTR_5g073660 | 82.353 | 34 | 6 | 0 | 10 | 43 | 29 | 62 | 7.83e-12 | 66.2 |
| MS.gene060258.t1 | MTR_5g073840 | 62.222 | 45 | 17 | 0 | 1 | 45 | 1 | 45 | 2.16e-11 | 64.3 |
| MS.gene060258.t1 | MTR_4g019260 | 46.552 | 58 | 31 | 0 | 202 | 259 | 10 | 67 | 4.02e-11 | 58.9 |
| MS.gene060258.t1 | MTR_5g073880 | 64.286 | 42 | 15 | 0 | 4 | 45 | 5 | 46 | 4.04e-11 | 63.5 |
| MS.gene060258.t1 | MTR_5g073860 | 64.286 | 42 | 15 | 0 | 4 | 45 | 18 | 59 | 7.03e-11 | 63.9 |
| MS.gene060258.t1 | MTR_5g074190 | 33.333 | 144 | 81 | 4 | 257 | 397 | 184 | 315 | 8.50e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060258.t1 | AT5G58430 | 62.069 | 290 | 98 | 2 | 115 | 404 | 189 | 466 | 1.89e-120 | 363 |
| MS.gene060258.t1 | AT1G07000 | 48.973 | 292 | 135 | 3 | 115 | 404 | 171 | 450 | 1.59e-84 | 270 |
| MS.gene060258.t1 | AT5G03540 | 34.520 | 281 | 171 | 2 | 125 | 404 | 87 | 355 | 1.14e-47 | 171 |
| MS.gene060258.t1 | AT5G03540 | 34.520 | 281 | 171 | 2 | 125 | 404 | 202 | 470 | 7.17e-47 | 170 |
| MS.gene060258.t1 | AT5G03540 | 34.520 | 281 | 171 | 2 | 125 | 404 | 228 | 496 | 1.12e-46 | 170 |
| MS.gene060258.t1 | AT1G72470 | 31.930 | 285 | 182 | 3 | 122 | 397 | 178 | 459 | 1.13e-45 | 167 |
| MS.gene060258.t1 | AT5G50380 | 31.618 | 272 | 178 | 3 | 126 | 397 | 240 | 503 | 1.84e-43 | 161 |
| MS.gene060258.t1 | AT1G54090 | 30.877 | 285 | 183 | 3 | 122 | 397 | 170 | 449 | 1.10e-41 | 155 |
| MS.gene060258.t1 | AT5G52340 | 32.967 | 273 | 171 | 1 | 125 | 397 | 202 | 462 | 2.76e-41 | 155 |
| MS.gene060258.t1 | AT5G13990 | 31.034 | 348 | 223 | 6 | 51 | 397 | 192 | 523 | 6.66e-40 | 151 |
| MS.gene060258.t1 | AT3G14090 | 28.521 | 284 | 192 | 3 | 128 | 404 | 179 | 458 | 2.70e-39 | 149 |
| MS.gene060258.t1 | AT5G13150 | 32.180 | 289 | 179 | 3 | 128 | 404 | 210 | 493 | 9.15e-39 | 148 |
| MS.gene060258.t1 | AT1G07725 | 28.873 | 284 | 187 | 5 | 137 | 411 | 184 | 461 | 8.52e-37 | 142 |
| MS.gene060258.t1 | AT3G29400 | 25.839 | 298 | 200 | 4 | 119 | 397 | 194 | 489 | 4.38e-32 | 129 |
| MS.gene060258.t1 | AT5G59730 | 28.041 | 296 | 202 | 6 | 127 | 411 | 164 | 459 | 1.57e-30 | 124 |
| MS.gene060258.t1 | AT5G59730 | 28.041 | 296 | 202 | 6 | 127 | 411 | 164 | 459 | 2.02e-30 | 124 |
| MS.gene060258.t1 | AT2G28640 | 25.795 | 283 | 200 | 3 | 123 | 397 | 150 | 430 | 2.47e-30 | 123 |
| MS.gene060258.t1 | AT5G52350 | 28.205 | 273 | 184 | 1 | 125 | 397 | 156 | 416 | 2.82e-30 | 123 |
| MS.gene060258.t1 | AT5G61010 | 27.675 | 271 | 185 | 4 | 133 | 397 | 215 | 480 | 1.58e-28 | 118 |
| MS.gene060258.t1 | AT5G61010 | 27.675 | 271 | 185 | 4 | 133 | 397 | 215 | 480 | 1.58e-28 | 118 |
| MS.gene060258.t1 | AT2G39380 | 25.899 | 278 | 192 | 3 | 130 | 397 | 183 | 456 | 1.74e-27 | 115 |
| MS.gene060258.t1 | AT2G28650 | 25.843 | 267 | 183 | 3 | 137 | 397 | 156 | 413 | 2.24e-27 | 114 |
| MS.gene060258.t1 | AT3G09520 | 29.075 | 227 | 155 | 2 | 115 | 335 | 155 | 381 | 3.11e-26 | 111 |
| MS.gene060258.t1 | AT3G09530 | 29.703 | 202 | 139 | 1 | 137 | 335 | 174 | 375 | 1.55e-25 | 109 |
| MS.gene060258.t1 | AT3G55150 | 26.990 | 289 | 200 | 5 | 130 | 409 | 184 | 470 | 3.47e-25 | 108 |
Find 102 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAGTTTGAAGAAGATTT+TGG | 0.106248 | 5.4:-60204359 | MS.gene060258:CDS |
| ATGCACTCTATCCTCAATTT+TGG | 0.137625 | 5.4:+60204316 | None:intergenic |
| CAGTTACAGGAGGGAATATT+TGG | 0.189792 | 5.4:-60204936 | MS.gene060258:CDS |
| AGTAGGGTCCTCGTAGATTT+TGG | 0.216151 | 5.4:+60204223 | None:intergenic |
| AAATCTTATCGGAATGCTTT+TGG | 0.224265 | 5.4:-60205119 | MS.gene060258:CDS |
| CCGGAAACTGCTGCCTTTGC+TGG | 0.241109 | 5.4:+60204458 | None:intergenic |
| AGTGAAGATGGGTTTGATTA+TGG | 0.242259 | 5.4:-60204276 | MS.gene060258:CDS |
| AAAAGCATTCCGATAAGATT+TGG | 0.270775 | 5.4:+60205121 | None:intergenic |
| CCAGCAAAGGCAGCAGTTTC+CGG | 0.273611 | 5.4:-60204458 | MS.gene060258:CDS |
| TGCTCATGCAAACTGTAAAA+TGG | 0.274159 | 5.4:-60205798 | MS.gene060258:CDS |
| GAATACCTTAGCGATGATTC+TGG | 0.302254 | 5.4:-60205085 | MS.gene060258:CDS |
| TCTTCCTTCAATTATCTGTT+TGG | 0.319564 | 5.4:-60205700 | MS.gene060258:intron |
| GGAGCTATTAGAGAGCATTT+TGG | 0.326513 | 5.4:-60204255 | MS.gene060258:CDS |
| TATCCTTCACGGATGTTTGC+AGG | 0.329769 | 5.4:-60204724 | MS.gene060258:CDS |
| TCGACGTGTATATCAGTTAC+AGG | 0.337038 | 5.4:-60204949 | MS.gene060258:CDS |
| TTCAACACGTAATGGGTAAT+CGG | 0.348059 | 5.4:+60204422 | None:intergenic |
| CAAGAAAATCCAAATCTTAT+CGG | 0.350480 | 5.4:-60205130 | MS.gene060258:CDS |
| GTAGAAAGCTTATCAAGATT+AGG | 0.355489 | 5.4:-60204914 | MS.gene060258:CDS |
| CTTTCATCTTCTAAACTAGT+AGG | 0.359405 | 5.4:+60205256 | None:intergenic |
| ACAACAAAGCAACAACAATT+TGG | 0.361181 | 5.4:-60204544 | MS.gene060258:CDS |
| TAGAAAGCTTATCAAGATTA+GGG | 0.361927 | 5.4:-60204913 | MS.gene060258:CDS |
| AACAACAATTTGGAAGAGAT+TGG | 0.368141 | 5.4:-60204534 | MS.gene060258:CDS |
| TACTGATATTGGTAGTAGTA+TGG | 0.368865 | 5.4:-60205188 | MS.gene060258:CDS |
| CACTGCTGATTTATCCTTCA+CGG | 0.374636 | 5.4:-60204735 | MS.gene060258:CDS |
| TCACAAAACAGCAAAGTTGA+TGG | 0.385033 | 5.4:-60204999 | MS.gene060258:CDS |
| CTGCTGCCTTTGCTGGATCT+TGG | 0.387509 | 5.4:+60204465 | None:intergenic |
| AAGGCTTTGATCATGAAGTC+TGG | 0.398018 | 5.4:+60205046 | None:intergenic |
| ACAACAATTTGGAAGAGATT+GGG | 0.400521 | 5.4:-60204533 | MS.gene060258:CDS |
| CGAAACATGGACTTGAACTC+TGG | 0.423943 | 5.4:+60204590 | None:intergenic |
| CAGTTGGGCGGTACTGATAT+TGG | 0.432419 | 5.4:-60205199 | MS.gene060258:CDS |
| CCAAAGGTATGGAGATTTGT+GGG | 0.434378 | 5.4:-60205766 | MS.gene060258:CDS |
| TGAACGGAAACTTTGTGATC+GGG | 0.445015 | 5.4:-60204777 | MS.gene060258:CDS |
| AAGGTAGTTCAACACGTAAT+GGG | 0.457041 | 5.4:+60204415 | None:intergenic |
| AAGGCAGCAGTTTCCGGTGT+TGG | 0.464676 | 5.4:-60204452 | MS.gene060258:CDS |
| TTCCTCTCTATCAGTGAAGA+TGG | 0.465906 | 5.4:-60204288 | MS.gene060258:CDS |
| AGCCTTGCCAACTGAAACAA+TGG | 0.468338 | 5.4:-60205029 | MS.gene060258:CDS |
| CTCACCAAACAGATAATTGA+AGG | 0.468970 | 5.4:+60205696 | None:intergenic |
| GAAGGTAGTTCAACACGTAA+TGG | 0.475458 | 5.4:+60204414 | None:intergenic |
| CTTCACTGATAGAGAGGAAG+TGG | 0.479915 | 5.4:+60204292 | None:intergenic |
| CCCACAAATCTCCATACCTT+TGG | 0.480790 | 5.4:+60205766 | None:intergenic |
| GTCCATTGTTTCAGTTGGCA+AGG | 0.483309 | 5.4:+60205027 | None:intergenic |
| TGACAACATCTGAACGTCTT+CGG | 0.483647 | 5.4:+60204874 | None:intergenic |
| GGAAAACATAGCACTGAAGT+AGG | 0.485132 | 5.4:+60204206 | None:intergenic |
| CAGCAGGTACAGTGTTCAAA+AGG | 0.485488 | 5.4:-60204173 | MS.gene060258:CDS |
| TTGAAGAAGAGATTGAAAGA+TGG | 0.488391 | 5.4:-60204841 | MS.gene060258:CDS |
| ATCACAAAGTTTCCGTTCAG+TGG | 0.495687 | 5.4:+60204781 | None:intergenic |
| CTGAACGGAAACTTTGTGAT+CGG | 0.497291 | 5.4:-60204778 | MS.gene060258:CDS |
| TTGTCAGTCACGGATGGTGC+TGG | 0.503030 | 5.4:-60204384 | MS.gene060258:CDS |
| TGTACCTGCTGTAATTCATC+AGG | 0.503183 | 5.4:+60204185 | None:intergenic |
| TTTCATCTTCTAAACTAGTA+GGG | 0.514168 | 5.4:+60205257 | None:intergenic |
| ACCAAAGGTATGGAGATTTG+TGG | 0.514902 | 5.4:-60205767 | MS.gene060258:CDS |
| GCAAAGTTGATGGTTAGTGC+AGG | 0.515007 | 5.4:-60204989 | MS.gene060258:CDS |
| TCAACACGTAATGGGTAATC+GGG | 0.521709 | 5.4:+60204423 | None:intergenic |
| GGGTGATGTCACCAAAGGTA+TGG | 0.527039 | 5.4:-60205777 | MS.gene060258:CDS |
| CATTCATATTCATCACAGTT+GGG | 0.527750 | 5.4:-60205214 | MS.gene060258:CDS |
| ATGCCGTCCATTGTTTCAGT+TGG | 0.529072 | 5.4:+60205022 | None:intergenic |
| TCCTCTCTATCAGTGAAGAT+GGG | 0.529089 | 5.4:-60204287 | MS.gene060258:CDS |
| CCATTCATATTCATCACAGT+TGG | 0.531008 | 5.4:-60205215 | MS.gene060258:CDS |
| AGGCTTTGATCATGAAGTCT+GGG | 0.531816 | 5.4:+60205047 | None:intergenic |
| GCTCATGCAAACTGTAAAAT+GGG | 0.532751 | 5.4:-60205797 | MS.gene060258:CDS |
| ATCCTTCACGGATGTTTGCA+GGG | 0.539926 | 5.4:-60204723 | MS.gene060258:CDS |
| GAAGGAATATCCCAAAATTG+AGG | 0.542073 | 5.4:-60204327 | MS.gene060258:CDS |
| TAAACAACCGCTCAGGTGAT+CGG | 0.543038 | 5.4:+60204651 | None:intergenic |
| CAACAATTTGGAAGAGATTG+GGG | 0.543080 | 5.4:-60204532 | MS.gene060258:CDS |
| ACGGTATTGATCACGAAACA+TGG | 0.549210 | 5.4:+60204577 | None:intergenic |
| CAAAGTTGATGGTTAGTGCA+GGG | 0.555798 | 5.4:-60204988 | MS.gene060258:CDS |
| AGATATTATTTCCCACTGAA+CGG | 0.563648 | 5.4:-60204793 | MS.gene060258:CDS |
| CTTTGTTGTGCAGCGACTGA+CGG | 0.564182 | 5.4:+60204558 | None:intergenic |
| TTTACAGTTTGCATGAGCAT+CGG | 0.567219 | 5.4:+60205802 | None:intergenic |
| TCACAAAGTTTCCGTTCAGT+GGG | 0.570711 | 5.4:+60204782 | None:intergenic |
| CGTTCAGATGTTGTCATGGA+AGG | 0.571479 | 5.4:-60204867 | MS.gene060258:CDS |
| TTTGCCGATGCTATTGCTAG+TGG | 0.574984 | 5.4:-60204677 | MS.gene060258:CDS |
| GGTTAGTGCAGGGTTTGAGA+AGG | 0.577226 | 5.4:-60204978 | MS.gene060258:CDS |
| CCAACTGTGATGAATATGAA+TGG | 0.582865 | 5.4:+60205215 | None:intergenic |
| ACCCATCTTCACTGATAGAG+AGG | 0.584934 | 5.4:+60204286 | None:intergenic |
| GAAAACATAGCACTGAAGTA+GGG | 0.592856 | 5.4:+60204207 | None:intergenic |
| TAAATCCAGAATCATCGCTA+AGG | 0.600639 | 5.4:+60205080 | None:intergenic |
| TGCTGATTGTCAGTCACGGA+TGG | 0.600899 | 5.4:-60204390 | MS.gene060258:CDS |
| ATGACTCTAAACAACCGCTC+AGG | 0.601313 | 5.4:+60204644 | None:intergenic |
| TTCCCTGCAAACATCCGTGA+AGG | 0.612227 | 5.4:+60204721 | None:intergenic |
| GTGACTGACAATCAGCATGA+AGG | 0.619865 | 5.4:+60204396 | None:intergenic |
| AAGACGTTCAGATGTTGTCA+TGG | 0.622352 | 5.4:-60204871 | MS.gene060258:CDS |
| TTCATGCTGATTGTCAGTCA+CGG | 0.623312 | 5.4:-60204394 | MS.gene060258:CDS |
| GAAGGATAAATCAGCAGTGG+AGG | 0.628489 | 5.4:+60204739 | None:intergenic |
| AAAATTCAGCAGCTGAAGTG+CGG | 0.630978 | 5.4:+60204697 | None:intergenic |
| CGTGAAGGATAAATCAGCAG+TGG | 0.632684 | 5.4:+60204736 | None:intergenic |
| TCAGTCACGGATGGTGCTGG+AGG | 0.634051 | 5.4:-60204381 | MS.gene060258:CDS |
| ACAACTTAGTGATTATGTGA+AGG | 0.636749 | 5.4:-60205161 | MS.gene060258:CDS |
| TGATATTGGTAGTAGTATGG+TGG | 0.641647 | 5.4:-60205185 | MS.gene060258:CDS |
| GCTTCCACTAGCAATAGCAT+CGG | 0.641991 | 5.4:+60204673 | None:intergenic |
| GATTCGCCAAGATCCAGCAA+AGG | 0.649881 | 5.4:-60204471 | MS.gene060258:CDS |
| AACAATTTGGAAGAGATTGG+GGG | 0.658750 | 5.4:-60204531 | MS.gene060258:CDS |
| ACGTGTATATCAGTTACAGG+AGG | 0.665468 | 5.4:-60204946 | MS.gene060258:CDS |
| CGTGTATATCAGTTACAGGA+GGG | 0.666664 | 5.4:-60204945 | MS.gene060258:CDS |
| AAAATGGGTGATGTCACCAA+AGG | 0.672395 | 5.4:-60205782 | MS.gene060258:CDS |
| TTGCCAACTGAAACAATGGA+CGG | 0.672416 | 5.4:-60205025 | MS.gene060258:CDS |
| TAATATCTTCAAAGCCACGT+TGG | 0.673863 | 5.4:+60204808 | None:intergenic |
| TCATATTCATCACAGTTGGG+CGG | 0.677437 | 5.4:-60205211 | MS.gene060258:CDS |
| AATCGGGTGAAGTCCAACAC+CGG | 0.686811 | 5.4:+60204439 | None:intergenic |
| ATGGATTAAAATTTCCAACG+TGG | 0.715635 | 5.4:-60204822 | MS.gene060258:CDS |
| GTGGAAGCCGATCACCTGAG+CGG | 0.733440 | 5.4:-60204658 | MS.gene060258:CDS |
| AGCCAAATCCAAAATCTACG+AGG | 0.755525 | 5.4:-60204231 | MS.gene060258:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGCAGTTATTCATTGT+TGG | - | chr5.4:60204622-60204641 | MS.gene060258:CDS | 25.0% |
| ! | CAAGAAAATCCAAATCTTAT+CGG | - | chr5.4:60204847-60204866 | MS.gene060258:CDS | 25.0% |
| ! | TAGAAAGCTTATCAAGATTA+GGG | - | chr5.4:60205064-60205083 | MS.gene060258:CDS | 25.0% |
| ! | TTTCATCTTCTAAACTAGTA+GGG | + | chr5.4:60204723-60204742 | None:intergenic | 25.0% |
| !! | AAGAGTTTGAAGAAGATTTT+GGG | - | chr5.4:60205619-60205638 | MS.gene060258:intron | 25.0% |
| !! | ATCTAAAACAACATCTATAG+AGG | + | chr5.4:60204692-60204711 | None:intergenic | 25.0% |
| !!! | GATCTTCTTTACTTTTTTCT+CGG | - | chr5.4:60204571-60204590 | MS.gene060258:CDS | 25.0% |
| !!! | TGATTTTGTTTGCAAAGATA+TGG | - | chr5.4:60204359-60204378 | MS.gene060258:CDS | 25.0% |
| !!! | TTCATTTTTCTTCGATAAAG+TGG | - | chr5.4:60204450-60204469 | MS.gene060258:CDS | 25.0% |
| AACAACAATTTGGAAGAGAT+TGG | - | chr5.4:60205443-60205462 | MS.gene060258:intron | 30.0% | |
| ACAACAAAGCAACAACAATT+TGG | - | chr5.4:60205433-60205452 | MS.gene060258:intron | 30.0% | |
| ACAACAATTTGGAAGAGATT+GGG | - | chr5.4:60205444-60205463 | MS.gene060258:intron | 30.0% | |
| ACAACTTAGTGATTATGTGA+AGG | - | chr5.4:60204816-60204835 | MS.gene060258:CDS | 30.0% | |
| AGATATTATTTCCCACTGAA+CGG | - | chr5.4:60205184-60205203 | MS.gene060258:CDS | 30.0% | |
| CATTCATATTCATCACAGTT+GGG | - | chr5.4:60204763-60204782 | MS.gene060258:CDS | 30.0% | |
| CTTTCATCTTCTAAACTAGT+AGG | + | chr5.4:60204724-60204743 | None:intergenic | 30.0% | |
| GTAGAAAGCTTATCAAGATT+AGG | - | chr5.4:60205063-60205082 | MS.gene060258:CDS | 30.0% | |
| TCTTCCTTCAATTATCTGTT+TGG | - | chr5.4:60204277-60204296 | MS.gene060258:CDS | 30.0% | |
| TTGAAGAAGAGATTGAAAGA+TGG | - | chr5.4:60205136-60205155 | MS.gene060258:CDS | 30.0% | |
| ! | AAATCTTATCGGAATGCTTT+TGG | - | chr5.4:60204858-60204877 | MS.gene060258:CDS | 30.0% |
| ! | ATGGATTAAAATTTCCAACG+TGG | - | chr5.4:60205155-60205174 | MS.gene060258:CDS | 30.0% |
| ! | GAAGAGTTTGAAGAAGATTT+TGG | - | chr5.4:60205618-60205637 | MS.gene060258:intron | 30.0% |
| ! | TACTGATATTGGTAGTAGTA+TGG | - | chr5.4:60204789-60204808 | MS.gene060258:CDS | 30.0% |
| !! | AAAAGCATTCCGATAAGATT+TGG | + | chr5.4:60204859-60204878 | None:intergenic | 30.0% |
| !!! | ATTTTGGGATATTCCTTCAA+CGG | + | chr5.4:60205648-60205667 | None:intergenic | 30.0% |
| !!! | TTTGTGATCGGGTTTTATTT+GGG | - | chr5.4:60205211-60205230 | MS.gene060258:CDS | 30.0% |
| AACAATTTGGAAGAGATTGG+GGG | - | chr5.4:60205446-60205465 | MS.gene060258:intron | 35.0% | |
| AAGGTAGTTCAACACGTAAT+GGG | + | chr5.4:60205565-60205584 | None:intergenic | 35.0% | |
| ACTCAATAACATGGTAGCAA+AGG | + | chr5.4:60204518-60204537 | None:intergenic | 35.0% | |
| CAACAATTTGGAAGAGATTG+GGG | - | chr5.4:60205445-60205464 | MS.gene060258:intron | 35.0% | |
| CAGTTATTCATTGTTGGAGT+AGG | - | chr5.4:60204628-60204647 | MS.gene060258:CDS | 35.0% | |
| CCAACTGTGATGAATATGAA+TGG | + | chr5.4:60204765-60204784 | None:intergenic | 35.0% | |
| CCATGTTATTGAGTTTGTCA+CGG | - | chr5.4:60204524-60204543 | MS.gene060258:CDS | 35.0% | |
| CCATTCATATTCATCACAGT+TGG | - | chr5.4:60204762-60204781 | MS.gene060258:CDS | 35.0% | |
| CTCACCAAACAGATAATTGA+AGG | + | chr5.4:60204284-60204303 | None:intergenic | 35.0% | |
| GAAGGAATATCCCAAAATTG+AGG | - | chr5.4:60205650-60205669 | MS.gene060258:intron | 35.0% | |
| GCTCATGCAAACTGTAAAAT+GGG | - | chr5.4:60204180-60204199 | MS.gene060258:CDS | 35.0% | |
| TAAATCCAGAATCATCGCTA+AGG | + | chr5.4:60204900-60204919 | None:intergenic | 35.0% | |
| TAATATCTTCAAAGCCACGT+TGG | + | chr5.4:60205172-60205191 | None:intergenic | 35.0% | |
| TCACAAAACAGCAAAGTTGA+TGG | - | chr5.4:60204978-60204997 | MS.gene060258:CDS | 35.0% | |
| TGCTCATGCAAACTGTAAAA+TGG | - | chr5.4:60204179-60204198 | MS.gene060258:CDS | 35.0% | |
| TTTACAGTTTGCATGAGCAT+CGG | + | chr5.4:60204178-60204197 | None:intergenic | 35.0% | |
| ! | AGTGAAGATGGGTTTGATTA+TGG | - | chr5.4:60205701-60205720 | MS.gene060258:CDS | 35.0% |
| ! | ATGCACTCTATCCTCAATTT+TGG | + | chr5.4:60205664-60205683 | None:intergenic | 35.0% |
| ! | CAAAGCTCACACAACATTTT+TGG | - | chr5.4:60204405-60204424 | MS.gene060258:CDS | 35.0% |
| ! | GAAAACATAGCACTGAAGTA+GGG | + | chr5.4:60205773-60205792 | None:intergenic | 35.0% |
| ! | GGTTTTGCTTCATCTATTCT+TGG | - | chr5.4:60204232-60204251 | MS.gene060258:CDS | 35.0% |
| ! | TCAATTATCTGTTTGGTGAG+TGG | - | chr5.4:60204284-60204303 | MS.gene060258:CDS | 35.0% |
| ! | TGATATTGGTAGTAGTATGG+TGG | - | chr5.4:60204792-60204811 | MS.gene060258:CDS | 35.0% |
| ! | TGCACTCTATCCTCAATTTT+GGG | + | chr5.4:60205663-60205682 | None:intergenic | 35.0% |
| ! | TTTTCCTGATGAATTACAGC+AGG | - | chr5.4:60205788-60205807 | MS.gene060258:CDS | 35.0% |
| !! | TTCAACACGTAATGGGTAAT+CGG | + | chr5.4:60205558-60205577 | None:intergenic | 35.0% |
| !!! | CTTTGTGATCGGGTTTTATT+TGG | - | chr5.4:60205210-60205229 | MS.gene060258:CDS | 35.0% |
| AAAATGGGTGATGTCACCAA+AGG | - | chr5.4:60204195-60204214 | MS.gene060258:CDS | 40.0% | |
| AAAATTCAGCAGCTGAAGTG+CGG | + | chr5.4:60205283-60205302 | None:intergenic | 40.0% | |
| AAGACGTTCAGATGTTGTCA+TGG | - | chr5.4:60205106-60205125 | MS.gene060258:CDS | 40.0% | |
| ACGTGTATATCAGTTACAGG+AGG | - | chr5.4:60205031-60205050 | MS.gene060258:CDS | 40.0% | |
| AGCCAAATCCAAAATCTACG+AGG | - | chr5.4:60205746-60205765 | MS.gene060258:CDS | 40.0% | |
| ATCACAAAGTTTCCGTTCAG+TGG | + | chr5.4:60205199-60205218 | None:intergenic | 40.0% | |
| CAGTTACAGGAGGGAATATT+TGG | - | chr5.4:60205041-60205060 | MS.gene060258:CDS | 40.0% | |
| CCGTGACAAACTCAATAACA+TGG | + | chr5.4:60204527-60204546 | None:intergenic | 40.0% | |
| CGTGTATATCAGTTACAGGA+GGG | - | chr5.4:60205032-60205051 | MS.gene060258:CDS | 40.0% | |
| CTGAACGGAAACTTTGTGAT+CGG | - | chr5.4:60205199-60205218 | MS.gene060258:CDS | 40.0% | |
| GAAGGTAGTTCAACACGTAA+TGG | + | chr5.4:60205566-60205585 | None:intergenic | 40.0% | |
| GAATACCTTAGCGATGATTC+TGG | - | chr5.4:60204892-60204911 | MS.gene060258:CDS | 40.0% | |
| TCACAAAGTTTCCGTTCAGT+GGG | + | chr5.4:60205198-60205217 | None:intergenic | 40.0% | |
| TCATATTCATCACAGTTGGG+CGG | - | chr5.4:60204766-60204785 | MS.gene060258:CDS | 40.0% | |
| TCCTCTCTATCAGTGAAGAT+GGG | - | chr5.4:60205690-60205709 | MS.gene060258:intron | 40.0% | |
| TCGACGTGTATATCAGTTAC+AGG | - | chr5.4:60205028-60205047 | MS.gene060258:CDS | 40.0% | |
| TGAACGGAAACTTTGTGATC+GGG | - | chr5.4:60205200-60205219 | MS.gene060258:CDS | 40.0% | |
| TGACAACATCTGAACGTCTT+CGG | + | chr5.4:60205106-60205125 | None:intergenic | 40.0% | |
| TGTACCTGCTGTAATTCATC+AGG | + | chr5.4:60205795-60205814 | None:intergenic | 40.0% | |
| TTCCTCTCTATCAGTGAAGA+TGG | - | chr5.4:60205689-60205708 | MS.gene060258:intron | 40.0% | |
| TTCTTCGATAAAGTGGTGAG+TGG | - | chr5.4:60204457-60204476 | MS.gene060258:CDS | 40.0% | |
| TTGCCAACTGAAACAATGGA+CGG | - | chr5.4:60204952-60204971 | MS.gene060258:CDS | 40.0% | |
| ! | AAGGCTTTGATCATGAAGTC+TGG | + | chr5.4:60204934-60204953 | None:intergenic | 40.0% |
| ! | ACCAAAGGTATGGAGATTTG+TGG | - | chr5.4:60204210-60204229 | MS.gene060258:CDS | 40.0% |
| ! | AGGCTTTGATCATGAAGTCT+GGG | + | chr5.4:60204933-60204952 | None:intergenic | 40.0% |
| ! | CAAAGGAAGCACATGAAATG+AGG | + | chr5.4:60204501-60204520 | None:intergenic | 40.0% |
| ! | CCAAAGGTATGGAGATTTGT+GGG | - | chr5.4:60204211-60204230 | MS.gene060258:CDS | 40.0% |
| ! | GGAAAACATAGCACTGAAGT+AGG | + | chr5.4:60205774-60205793 | None:intergenic | 40.0% |
| ! | GTCCTCGTAGATTTTGGATT+TGG | + | chr5.4:60205751-60205770 | None:intergenic | 40.0% |
| ! | TTCATGCTGATTGTCAGTCA+CGG | - | chr5.4:60205583-60205602 | MS.gene060258:intron | 40.0% |
| !! | ACGGTATTGATCACGAAACA+TGG | + | chr5.4:60205403-60205422 | None:intergenic | 40.0% |
| !! | AGATTTTGGGTATCCGTTGA+AGG | - | chr5.4:60205632-60205651 | MS.gene060258:intron | 40.0% |
| !! | CAAAGTTGATGGTTAGTGCA+GGG | - | chr5.4:60204989-60205008 | MS.gene060258:CDS | 40.0% |
| !! | CACTGCTGATTTATCCTTCA+CGG | - | chr5.4:60205242-60205261 | MS.gene060258:CDS | 40.0% |
| !! | TCAACACGTAATGGGTAATC+GGG | + | chr5.4:60205557-60205576 | None:intergenic | 40.0% |
| !!! | GGAGCTATTAGAGAGCATTT+TGG | - | chr5.4:60205722-60205741 | MS.gene060258:CDS | 40.0% |
| AACTCAGTGCGGATTTGAAG+TGG | - | chr5.4:60204549-60204568 | MS.gene060258:CDS | 45.0% | |
| ACCCATCTTCACTGATAGAG+AGG | + | chr5.4:60205694-60205713 | None:intergenic | 45.0% | |
| AGCCTTGCCAACTGAAACAA+TGG | - | chr5.4:60204948-60204967 | MS.gene060258:CDS | 45.0% | |
| ATCCTTCACGGATGTTTGCA+GGG | - | chr5.4:60205254-60205273 | MS.gene060258:CDS | 45.0% | |
| ATGACTCTAAACAACCGCTC+AGG | + | chr5.4:60205336-60205355 | None:intergenic | 45.0% | |
| ATGCCGTCCATTGTTTCAGT+TGG | + | chr5.4:60204958-60204977 | None:intergenic | 45.0% | |
| CAGCAGGTACAGTGTTCAAA+AGG | - | chr5.4:60205804-60205823 | MS.gene060258:CDS | 45.0% | |
| CCCACAAATCTCCATACCTT+TGG | + | chr5.4:60204214-60204233 | None:intergenic | 45.0% | |
| CGAAACATGGACTTGAACTC+TGG | + | chr5.4:60205390-60205409 | None:intergenic | 45.0% | |
| CGTGAAGGATAAATCAGCAG+TGG | + | chr5.4:60205244-60205263 | None:intergenic | 45.0% | |
| CGTTCAGATGTTGTCATGGA+AGG | - | chr5.4:60205110-60205129 | MS.gene060258:CDS | 45.0% | |
| CTTCACTGATAGAGAGGAAG+TGG | + | chr5.4:60205688-60205707 | None:intergenic | 45.0% | |
| GAAATGAGGCTATACGCATC+TGG | + | chr5.4:60204487-60204506 | None:intergenic | 45.0% | |
| GAAGGATAAATCAGCAGTGG+AGG | + | chr5.4:60205241-60205260 | None:intergenic | 45.0% | |
| GCTTCCACTAGCAATAGCAT+CGG | + | chr5.4:60205307-60205326 | None:intergenic | 45.0% | |
| GTCCATTGTTTCAGTTGGCA+AGG | + | chr5.4:60204953-60204972 | None:intergenic | 45.0% | |
| GTGACTGACAATCAGCATGA+AGG | + | chr5.4:60205584-60205603 | None:intergenic | 45.0% | |
| TAAACAACCGCTCAGGTGAT+CGG | + | chr5.4:60205329-60205348 | None:intergenic | 45.0% | |
| TATCCTTCACGGATGTTTGC+AGG | - | chr5.4:60205253-60205272 | MS.gene060258:CDS | 45.0% | |
| TTTGCCGATGCTATTGCTAG+TGG | - | chr5.4:60205300-60205319 | MS.gene060258:intron | 45.0% | |
| ! | AGTAGGGTCCTCGTAGATTT+TGG | + | chr5.4:60205757-60205776 | None:intergenic | 45.0% |
| !! | GCAAAGTTGATGGTTAGTGC+AGG | - | chr5.4:60204988-60205007 | MS.gene060258:CDS | 45.0% |
| AAGAGATTGGGGGAAGCGAT+CGG | - | chr5.4:60205456-60205475 | MS.gene060258:intron | 50.0% | |
| AATCGGGTGAAGTCCAACAC+CGG | + | chr5.4:60205541-60205560 | None:intergenic | 50.0% | |
| GATTCGCCAAGATCCAGCAA+AGG | - | chr5.4:60205506-60205525 | MS.gene060258:intron | 50.0% | |
| GGGTGATGTCACCAAAGGTA+TGG | - | chr5.4:60204200-60204219 | MS.gene060258:CDS | 50.0% | |
| GGTTAGTGCAGGGTTTGAGA+AGG | - | chr5.4:60204999-60205018 | MS.gene060258:CDS | 50.0% | |
| TTCCCTGCAAACATCCGTGA+AGG | + | chr5.4:60205259-60205278 | None:intergenic | 50.0% | |
| TTGTCACGGCAAACTCAGTG+CGG | - | chr5.4:60204538-60204557 | MS.gene060258:CDS | 50.0% | |
| ! | CTTTGTTGTGCAGCGACTGA+CGG | + | chr5.4:60205422-60205441 | None:intergenic | 50.0% |
| ! | TGCTGATTGTCAGTCACGGA+TGG | - | chr5.4:60205587-60205606 | MS.gene060258:intron | 50.0% |
| !! | CAGTTGGGCGGTACTGATAT+TGG | - | chr5.4:60204778-60204797 | MS.gene060258:CDS | 50.0% |
| !!! | AGCGATCGGAGGCATTTTCA+TGG | - | chr5.4:60205470-60205489 | MS.gene060258:intron | 50.0% |
| !!! | CGGAGGCATTTTCATGGAGT+TGG | - | chr5.4:60205476-60205495 | MS.gene060258:intron | 50.0% |
| AAGGCAGCAGTTTCCGGTGT+TGG | - | chr5.4:60205525-60205544 | MS.gene060258:intron | 55.0% | |
| CCAGCAAAGGCAGCAGTTTC+CGG | - | chr5.4:60205519-60205538 | MS.gene060258:intron | 55.0% | |
| CTGCTGCCTTTGCTGGATCT+TGG | + | chr5.4:60205515-60205534 | None:intergenic | 55.0% | |
| TTGTCAGTCACGGATGGTGC+TGG | - | chr5.4:60205593-60205612 | MS.gene060258:intron | 55.0% | |
| AGATTGGGGGAAGCGATCGG+AGG | - | chr5.4:60205459-60205478 | MS.gene060258:intron | 60.0% | |
| CCGGAAACTGCTGCCTTTGC+TGG | + | chr5.4:60205522-60205541 | None:intergenic | 60.0% | |
| GTGGAAGCCGATCACCTGAG+CGG | - | chr5.4:60205319-60205338 | MS.gene060258:intron | 60.0% | |
| ! | TCAGTCACGGATGGTGCTGG+AGG | - | chr5.4:60205596-60205615 | MS.gene060258:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 60204169 | 60205830 | 60204169 | ID=MS.gene060258 |
| chr5.4 | mRNA | 60204169 | 60205830 | 60204169 | ID=MS.gene060258.t1;Parent=MS.gene060258 |
| chr5.4 | exon | 60205701 | 60205830 | 60205701 | ID=MS.gene060258.t1.exon1;Parent=MS.gene060258.t1 |
| chr5.4 | CDS | 60205701 | 60205830 | 60205701 | ID=cds.MS.gene060258.t1;Parent=MS.gene060258.t1 |
| chr5.4 | exon | 60204169 | 60205289 | 60204169 | ID=MS.gene060258.t1.exon2;Parent=MS.gene060258.t1 |
| chr5.4 | CDS | 60204169 | 60205289 | 60204169 | ID=cds.MS.gene060258.t1;Parent=MS.gene060258.t1 |
| Gene Sequence |
| Protein sequence |
