Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72219.t1 | XP_004492881.1 | 51.6 | 188 | 84 | 2 | 169 | 350 | 63 | 249 | 7.60E-39 | 171.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72219.t1 | A0A392PHL9 | 65.8 | 199 | 64 | 2 | 159 | 357 | 1 | 195 | 2.6e-65 | 258.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058537 | MS.gene72219 | 0.96773 | 1.11E-127 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72219.t1 | MTR_7g081150 | 42.063 | 126 | 64 | 2 | 225 | 350 | 206 | 322 | 8.37e-20 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 149 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATATTCAAATAATTGAT+AGG | + | 50753:4941-4960 | MS.gene72219:intron | 10.0% |
| !!! | AAAACATTGAAAACTATTTT+AGG | - | 50753:4468-4487 | MS.gene72219:intergenic | 15.0% |
| !!! | TAAAATAGTTTTCAATGTTT+TGG | + | 50753:4467-4486 | MS.gene72219:intron | 15.0% |
| !! | AAAAATAGAGGACAAAAATT+AGG | - | 50753:4244-4263 | MS.gene72219:intergenic | 20.0% |
| !! | ATTACTTGTACCTATATAAT+TGG | - | 50753:4714-4733 | MS.gene72219:intergenic | 20.0% |
| !!! | AAAGATTAGCTCAATATATA+AGG | + | 50753:5036-5055 | MS.gene72219:CDS | 20.0% |
| !!! | TTTCCTTTTTCTTTTTAGTA+AGG | - | 50753:4750-4769 | MS.gene72219:intergenic | 20.0% |
| !!! | TTTTTTGATTACCATGTTAT+AGG | + | 50753:4260-4279 | MS.gene72219:intron | 20.0% |
| ! | AAGAAAAAGGAAAACAGAAA+TGG | + | 50753:4757-4776 | MS.gene72219:intron | 25.0% |
| ! | ATTAAGCTTAGTTAGATGTA+GGG | - | 50753:4437-4456 | MS.gene72219:intergenic | 25.0% |
| ! | ATTTACAATCCATCTGTTTA+GGG | + | 50753:4292-4311 | MS.gene72219:CDS | 25.0% |
| ! | CATGGTAATCAAAAAAATAG+AGG | - | 50753:4256-4275 | MS.gene72219:intergenic | 25.0% |
| ! | GTAAAAACATTATCAAGCAT+CGG | - | 50753:4844-4863 | MS.gene72219:intergenic | 25.0% |
| ! | TAGAAGAAAAACAGTTAGAA+TGG | - | 50753:4178-4197 | MS.gene72219:intergenic | 25.0% |
| ! | TATCTTGCTACAAAAATGTT+AGG | + | 50753:5117-5136 | MS.gene72219:CDS | 25.0% |
| ! | TCACAATGAATCACAATATT+TGG | - | 50753:4636-4655 | MS.gene72219:intergenic | 25.0% |
| ! | TTCATTGTACTACTATTAGT+TGG | - | 50753:4683-4702 | MS.gene72219:intergenic | 25.0% |
| !! | AGAAAACTGAAATACAGTTT+TGG | + | 50753:5075-5094 | MS.gene72219:CDS | 25.0% |
| AACAGTATATCAAATCAAGC+AGG | - | 50753:4515-4534 | MS.gene72219:intergenic | 30.0% | |
| ACAAAACCAACAAATGGTTT+GGG | - | 50753:4413-4432 | MS.gene72219:intergenic | 30.0% | |
| ACAGAGCAAATTAAGGATAA+TGG | - | 50753:4204-4223 | MS.gene72219:intergenic | 30.0% | |
| ACTTGTACCTATATAATTGG+TGG | - | 50753:4711-4730 | MS.gene72219:intergenic | 30.0% | |
| AGGCCTTACTAAAAAGAAAA+AGG | + | 50753:4744-4763 | MS.gene72219:intron | 30.0% | |
| ATGTCTTCTTACCTATAACA+TGG | - | 50753:4274-4293 | MS.gene72219:intergenic | 30.0% | |
| CAAGATAACAGAGCAAATTA+AGG | - | 50753:4211-4230 | MS.gene72219:intergenic | 30.0% | |
| CATTAAGCTTAGTTAGATGT+AGG | - | 50753:4438-4457 | MS.gene72219:intergenic | 30.0% | |
| CATTTACAATCCATCTGTTT+AGG | + | 50753:4291-4310 | MS.gene72219:CDS | 30.0% | |
| TGATTGAAGATGCATCAAAA+TGG | - | 50753:5741-5760 | MS.gene72219:intergenic | 30.0% | |
| TGTACGAATTTGTGAAACTT+TGG | + | 50753:4539-4558 | MS.gene72219:intron | 30.0% | |
| TTAAGCTTAGTTAGATGTAG+GGG | - | 50753:4436-4455 | MS.gene72219:intergenic | 30.0% | |
| TTGGTATTTCCTACAACATA+TGG | + | 50753:5534-5553 | MS.gene72219:CDS | 30.0% | |
| ! | GAAAGCTATGCTTATCTTTT+TGG | + | 50753:5715-5734 | MS.gene72219:CDS | 30.0% |
| ! | GAAGTGTTTGTTACATTAGT+CGG | + | 50753:5776-5795 | MS.gene72219:CDS | 30.0% |
| !! | AACTGAAATACAGTTTTGGT+TGG | + | 50753:5079-5098 | MS.gene72219:CDS | 30.0% |
| AACAAGAACCATTACAGCAA+CGG | - | 50753:5672-5691 | MS.gene72219:intergenic | 35.0% | |
| AACACGACCACCAATTATAT+AGG | + | 50753:4701-4720 | MS.gene72219:intron | 35.0% | |
| AATGCTGTAAAATGGTTTCG+TGG | - | 50753:5290-5309 | MS.gene72219:intergenic | 35.0% | |
| AATTTCAAAGCAACACCTAC+AGG | - | 50753:3990-4009 | MS.gene72219:intergenic | 35.0% | |
| ATGCTGTAAAATGGTTTCGT+GGG | - | 50753:5289-5308 | MS.gene72219:intergenic | 35.0% | |
| ATTTCAAAGCAACACCTACA+GGG | - | 50753:3989-4008 | MS.gene72219:intergenic | 35.0% | |
| CAAAACCAACAAATGGTTTG+GGG | - | 50753:4412-4431 | MS.gene72219:intergenic | 35.0% | |
| GACAAAACCAACAAATGGTT+TGG | - | 50753:4414-4433 | MS.gene72219:intergenic | 35.0% | |
| GACAATCGAATAAGCATTAC+AGG | + | 50753:5347-5366 | MS.gene72219:CDS | 35.0% | |
| GCTTTCTGTATACTAGTGAA+TGG | - | 50753:5701-5720 | MS.gene72219:intergenic | 35.0% | |
| GGAAATACCAAGAAAGACAA+TGG | - | 50753:5525-5544 | MS.gene72219:intergenic | 35.0% | |
| TACAAGTAATGAGCTAAGTG+AGG | + | 50753:4724-4743 | MS.gene72219:intron | 35.0% | |
| TATATCAAATCAAGCAGGAG+TGG | - | 50753:4510-4529 | MS.gene72219:intergenic | 35.0% | |
| TATTGCGTATGGAGAAAGTT+TGG | - | 50753:4376-4395 | MS.gene72219:intergenic | 35.0% | |
| TGTAATCGTAGGTATAGTCA+TGG | - | 50753:5448-5467 | MS.gene72219:intergenic | 35.0% | |
| ! | GGACATGGATAATTGATAAC+TGG | - | 50753:5565-5584 | MS.gene72219:intergenic | 35.0% |
| AAAGGAAGCCTGAGATTACA+AGG | + | 50753:4090-4109 | MS.gene72219:CDS | 40.0% | |
| AAATGTAAACACATCCTCCC+CGG | - | 50753:4045-4064 | MS.gene72219:intergenic | 40.0% | |
| AACTTACCCAGATACTCGAA+AGG | - | 50753:5157-5176 | MS.gene72219:intergenic | 40.0% | |
| ATGCTGAAATACTTGCAACC+GGG | + | 50753:4024-4043 | MS.gene72219:CDS | 40.0% | |
| ATGTAAAGATGACAGCCTAC+CGG | - | 50753:4155-4174 | MS.gene72219:intergenic | 40.0% | |
| CCACTTCACAAAGAACAGAA+TGG | + | 50753:5587-5606 | MS.gene72219:CDS | 40.0% | |
| CCATTCTGTTCTTTGTGAAG+TGG | - | 50753:5590-5609 | MS.gene72219:intergenic | 40.0% | |
| CGAGTTTGATGAGAAGAATG+GGG | + | 50753:4998-5017 | MS.gene72219:CDS | 40.0% | |
| CGTATGGAGAAAGTTTGGTT+GGG | - | 50753:4371-4390 | MS.gene72219:intergenic | 40.0% | |
| CTACAATGAAGATTATCCGC+CGG | + | 50753:4133-4152 | MS.gene72219:CDS | 40.0% | |
| CTGGTTGTTCCATATGTTGT+AGG | - | 50753:5546-5565 | MS.gene72219:intergenic | 40.0% | |
| CTGTTTCACGTATCCTTCAA+AGG | + | 50753:4072-4091 | MS.gene72219:CDS | 40.0% | |
| GATGCTGAAATACTTGCAAC+CGG | + | 50753:4023-4042 | MS.gene72219:CDS | 40.0% | |
| GATTGTCTTTCATTGCCCAA+GGG | - | 50753:5334-5353 | MS.gene72219:intergenic | 40.0% | |
| GCGAGTTTGATGAGAAGAAT+GGG | + | 50753:4997-5016 | MS.gene72219:CDS | 40.0% | |
| GGCGGATAATCTTCATTGTA+GGG | - | 50753:4134-4153 | MS.gene72219:intergenic | 40.0% | |
| GGTATAGTCATGGTCAAACT+CGG | - | 50753:5438-5457 | MS.gene72219:intergenic | 40.0% | |
| GTATAGTCATGGTCAAACTC+GGG | - | 50753:5437-5456 | MS.gene72219:intergenic | 40.0% | |
| GTCTCAACCATTGTCTTTCT+TGG | + | 50753:5515-5534 | MS.gene72219:CDS | 40.0% | |
| GTTGCTGTAATGGTTCTTGT+TGG | + | 50753:5671-5690 | MS.gene72219:CDS | 40.0% | |
| TAAAACTGCATAGCAGCAGT+GGG | - | 50753:4799-4818 | MS.gene72219:intergenic | 40.0% | |
| TAATCTCAGGCTTCCTTTGA+AGG | - | 50753:4088-4107 | MS.gene72219:intergenic | 40.0% | |
| TAGGGGACAAAACCAACAAA+TGG | - | 50753:4419-4438 | MS.gene72219:intergenic | 40.0% | |
| TATAGTCATGGTCAAACTCG+GGG | - | 50753:5436-5455 | MS.gene72219:intergenic | 40.0% | |
| TCAAACCCCAAACCATTTGT+TGG | + | 50753:4404-4423 | MS.gene72219:intron | 40.0% | |
| TCAGGAAACCTTGTAATCTC+AGG | - | 50753:4101-4120 | MS.gene72219:intergenic | 40.0% | |
| TTAAAACTGCATAGCAGCAG+TGG | - | 50753:4800-4819 | MS.gene72219:intergenic | 40.0% | |
| TTACAATCAGCACATGCGTA+AGG | + | 50753:5463-5482 | MS.gene72219:CDS | 40.0% | |
| TTCCAGGGAATGCTGTAAAA+TGG | - | 50753:5298-5317 | MS.gene72219:intergenic | 40.0% | |
| TTCTCCATACGCAATAGTCA+GGG | + | 50753:4380-4399 | MS.gene72219:intron | 40.0% | |
| TTCTGTTCTTTGTGAAGTGG+CGG | - | 50753:5587-5606 | MS.gene72219:intergenic | 40.0% | |
| TTGTAGGGAAAACTGTCATC+AGG | - | 50753:4119-4138 | MS.gene72219:intergenic | 40.0% | |
| TTTCTCCATACGCAATAGTC+AGG | + | 50753:4379-4398 | MS.gene72219:intron | 40.0% | |
| ! | AACCATTTTACAGCATTCCC+TGG | + | 50753:5293-5312 | MS.gene72219:CDS | 40.0% |
| ! | AGTGATTTCCAGTGTGGTTT+TGG | + | 50753:5257-5276 | MS.gene72219:CDS | 40.0% |
| ! | ATTAGTCGGTGGACTTTCTA+TGG | + | 50753:5790-5809 | MS.gene72219:CDS | 40.0% |
| ! | GTGTTTGTTACATTAGTCGG+TGG | + | 50753:5779-5798 | MS.gene72219:CDS | 40.0% |
| ! | TCTATGGGGTGGTATTGTTT+TGG | + | 50753:5806-5825 | MS.gene72219:CDS | 40.0% |
| ! | TTAGTCGGTGGACTTTCTAT+GGG | + | 50753:5791-5810 | MS.gene72219:CDS | 40.0% |
| !! | CATGTGCTGATTGTAATCGT+AGG | - | 50753:5459-5478 | MS.gene72219:intergenic | 40.0% |
| AAAACCGAACAACCCAAGCA+AGG | - | 50753:5633-5652 | MS.gene72218:intergenic | 45.0% | |
| AAAACTGCATAGCAGCAGTG+GGG | - | 50753:4798-4817 | MS.gene72218:intergenic | 45.0% | |
| AAAATGTTAGGAGTCGTCCG+TGG | + | 50753:5129-5148 | MS.gene72219:CDS | 45.0% | |
| AAGTAGGCTTCCAATCCCTT+GGG | + | 50753:5316-5335 | MS.gene72219:CDS | 45.0% | |
| AATACGTGGCCCTAAACAGA+TGG | - | 50753:4304-4323 | MS.gene72219:intergenic | 45.0% | |
| AATGAAGATTATCCGCCGGT+AGG | + | 50753:4137-4156 | MS.gene72219:intron | 45.0% | |
| ACTCACCTTGAATGTCCAAC+CGG | - | 50753:4340-4359 | MS.gene72219:intergenic | 45.0% | |
| ACTTCAGACTTCATCCCTGT+AGG | + | 50753:3972-3991 | MS.gene72219:CDS | 45.0% | |
| CAGATACTCGAAAGGTTCCA+CGG | - | 50753:5149-5168 | MS.gene72219:intergenic | 45.0% | |
| CGATTGTCTTTCATTGCCCA+AGG | - | 50753:5335-5354 | MS.gene72219:intergenic | 45.0% | |
| CGGCGGATAATCTTCATTGT+AGG | - | 50753:4135-4154 | MS.gene72219:intergenic | 45.0% | |
| GAAAACCGGTTGGACATTCA+AGG | + | 50753:4332-4351 | MS.gene72219:CDS | 45.0% | |
| GCGTATGGAGAAAGTTTGGT+TGG | - | 50753:4372-4391 | MS.gene72219:intergenic | 45.0% | |
| GGCGAGTTTGATGAGAAGAA+TGG | + | 50753:4996-5015 | MS.gene72219:CDS | 45.0% | |
| GGGGAGGATGTGTTTACATT+TGG | + | 50753:4044-4063 | MS.gene72219:CDS | 45.0% | |
| TCTGTTCTTTGTGAAGTGGC+GGG | - | 50753:5586-5605 | MS.gene72219:intergenic | 45.0% | |
| TCTTTCATTGCCCAAGGGAT+TGG | - | 50753:5329-5348 | MS.gene72219:intergenic | 45.0% | |
| TGATCCCTGACTATTGCGTA+TGG | - | 50753:4387-4406 | MS.gene72219:intergenic | 45.0% | |
| TGCTGAAATACTTGCAACCG+GGG | + | 50753:4025-4044 | MS.gene72219:CDS | 45.0% | |
| TGGATGACAATGAGCTGCAT+TGG | + | 50753:5387-5406 | MS.gene72219:CDS | 45.0% | |
| TTACAGCATTCCCTGGAAGT+AGG | + | 50753:5300-5319 | MS.gene72219:CDS | 45.0% | |
| ! | GGAGTGTTAGAAGGTAAGTG+AGG | - | 50753:4823-4842 | MS.gene72219:intergenic | 45.0% |
| ! | GTGGAACCTTTCGAGTATCT+GGG | + | 50753:5148-5167 | MS.gene72219:CDS | 45.0% |
| ! | TAGTCGGTGGACTTTCTATG+GGG | + | 50753:5792-5811 | MS.gene72219:CDS | 45.0% |
| ! | TGTTTGTGCCGTTGCTGTAA+TGG | + | 50753:5661-5680 | MS.gene72219:CDS | 45.0% |
| !! | TCAAGCATCGGAGTGTTAGA+AGG | - | 50753:4832-4851 | MS.gene72219:intergenic | 45.0% |
| AAACTGCATAGCAGCAGTGG+GGG | - | 50753:4797-4816 | MS.gene72219:intergenic | 50.0% | |
| AGAATGGCCGCCTTTCTGTA+TGG | + | 50753:5603-5622 | MS.gene72219:CDS | 50.0% | |
| AGGAAACATGTTCACCCAGC+TGG | + | 50753:5367-5386 | MS.gene72219:CDS | 50.0% | |
| AGGACGACCATACAGAAAGG+CGG | - | 50753:5613-5632 | MS.gene72219:intergenic | 50.0% | |
| CAGCTCATTGTCATCCAGCT+GGG | - | 50753:5384-5403 | MS.gene72219:intergenic | 50.0% | |
| CTGCAGAGTGATTTCCAGTG+TGG | + | 50753:5251-5270 | MS.gene72219:intron | 50.0% | |
| CTGTATGGTCGTCCTTGCTT+GGG | + | 50753:5618-5637 | MS.gene72219:CDS | 50.0% | |
| GAAGTAGGCTTCCAATCCCT+TGG | + | 50753:5315-5334 | MS.gene72219:CDS | 50.0% | |
| GCAAGGACGACCATACAGAA+AGG | - | 50753:5616-5635 | MS.gene72219:intergenic | 50.0% | |
| GGATTGGAAGCCTACTTCCA+GGG | - | 50753:5313-5332 | MS.gene72219:intergenic | 50.0% | |
| GGCACTTTCCAAAACCACAC+TGG | - | 50753:5268-5287 | MS.gene72219:intergenic | 50.0% | |
| TAAAGATGACAGCCTACCGG+CGG | - | 50753:4152-4171 | MS.gene72219:intergenic | 50.0% | |
| TCTCATCAAACTCGCCACGT+AGG | - | 50753:4992-5011 | MS.gene72219:intergenic | 50.0% | |
| TCTGTATGGTCGTCCTTGCT+TGG | + | 50753:5617-5636 | MS.gene72219:CDS | 50.0% | |
| TGAAATACTTGCAACCGGGG+AGG | + | 50753:4028-4047 | MS.gene72219:CDS | 50.0% | |
| ! | CGTGGAACCTTTCGAGTATC+TGG | + | 50753:5147-5166 | MS.gene72219:CDS | 50.0% |
| ! | TCGTCCTTGCTTGGGTTGTT+CGG | + | 50753:5626-5645 | MS.gene72219:CDS | 50.0% |
| !! | AATGTCCAACCGGTTTTCCG+AGG | - | 50753:4330-4349 | MS.gene72219:intergenic | 50.0% |
| !! | ATGTCCAACCGGTTTTCCGA+GGG | - | 50753:4329-4348 | MS.gene72219:intergenic | 50.0% |
| AAACTCGCCACGTAGGAGGT+GGG | - | 50753:4985-5004 | MS.gene72219:intergenic | 55.0% | |
| AACTGCATAGCAGCAGTGGG+GGG | - | 50753:4796-4815 | MS.gene72219:intergenic | 55.0% | |
| CATCAAACTCGCCACGTAGG+AGG | - | 50753:4989-5008 | MS.gene72219:intergenic | 55.0% | |
| CGTATTCGCCCTCGGAAAAC+CGG | + | 50753:4318-4337 | MS.gene72219:CDS | 55.0% | |
| CGTTTAACCCACCTCCTACG+TGG | + | 50753:4975-4994 | MS.gene72219:CDS | 55.0% | |
| GCAGCTCATTGTCATCCAGC+TGG | - | 50753:5385-5404 | MS.gene72219:intergenic | 55.0% | |
| GGGATTGGAAGCCTACTTCC+AGG | - | 50753:5314-5333 | MS.gene72219:intergenic | 55.0% | |
| ! | CTTTGTGAAGTGGCGGGACA+TGG | - | 50753:5580-5599 | MS.gene72219:intergenic | 55.0% |
| ! | GTTTTCCGAGGGCGAATACG+TGG | - | 50753:4318-4337 | MS.gene72219:intergenic | 55.0% |
| ! | TCGGTGGACTTTCTATGGGG+TGG | + | 50753:5795-5814 | MS.gene72219:CDS | 55.0% |
| !! | CGTAGGAGGTGGGTTAAACG+AGG | - | 50753:4975-4994 | MS.gene72219:intergenic | 55.0% |
| CAAACTCGCCACGTAGGAGG+TGG | - | 50753:4986-5005 | MS.gene72219:intergenic | 60.0% | |
| TAGGGCCACGTATTCGCCCT+CGG | + | 50753:4310-4329 | MS.gene72219:CDS | 60.0% | |
| TTCGCCCTCGGAAAACCGGT+TGG | + | 50753:4322-4341 | MS.gene72219:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50753 | gene | 3960 | 5859 | 3960 | ID=MS.gene72219 |
| 50753 | mRNA | 3960 | 5859 | 3960 | ID=MS.gene72219.t1;Parent=MS.gene72219 |
| 50753 | exon | 3960 | 4154 | 3960 | ID=MS.gene72219.t1.exon1;Parent=MS.gene72219.t1 |
| 50753 | CDS | 3960 | 4154 | 3960 | ID=cds.MS.gene72219.t1;Parent=MS.gene72219.t1 |
| 50753 | exon | 4282 | 4353 | 4282 | ID=MS.gene72219.t1.exon2;Parent=MS.gene72219.t1 |
| 50753 | CDS | 4282 | 4353 | 4282 | ID=cds.MS.gene72219.t1;Parent=MS.gene72219.t1 |
| 50753 | exon | 4963 | 5169 | 4963 | ID=MS.gene72219.t1.exon3;Parent=MS.gene72219.t1 |
| 50753 | CDS | 4963 | 5169 | 4963 | ID=cds.MS.gene72219.t1;Parent=MS.gene72219.t1 |
| 50753 | exon | 5257 | 5859 | 5257 | ID=MS.gene72219.t1.exon4;Parent=MS.gene72219.t1 |
| 50753 | CDS | 5257 | 5859 | 5257 | ID=cds.MS.gene72219.t1;Parent=MS.gene72219.t1 |
| Gene Sequence |
| Protein sequence |
