Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72251.t1 | XP_013453119.1 | 97.2 | 143 | 4 | 0 | 1 | 143 | 100 | 242 | 1.10E-76 | 295.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72251.t1 | Q9ZSY8 | 74.2 | 151 | 30 | 3 | 2 | 143 | 143 | 293 | 4.7e-57 | 221.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72251.t1 | A0A072UBZ5 | 97.2 | 143 | 4 | 0 | 1 | 143 | 100 | 242 | 7.7e-77 | 295.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059607 | MS.gene72251 | 0.932315 | 1.02E-94 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACATAAAGGCATCCAAATT+TGG | 0.170065 | 6.3:-81427392 | None:intergenic |
| AGATCCACTTTCCTCAAGTA+TGG | 0.227669 | 6.3:-81427436 | None:intergenic |
| GGCCACCAATCCGTTCTTTC+AGG | 0.268848 | 6.3:+81427317 | MS.gene72251:CDS |
| GATGATGTAGAAGCCAAATT+TGG | 0.307716 | 6.3:+81427379 | MS.gene72251:CDS |
| ATGAAAAGTGTAGGTCAATT+TGG | 0.319033 | 6.3:+81427953 | MS.gene72251:intron |
| TTGACTCTTGCAAGAGGTTA+AGG | 0.334728 | 6.3:+81428504 | MS.gene72251:CDS |
| AGTTCTGAAGCAATTGGATT+AGG | 0.360095 | 6.3:+81428536 | MS.gene72251:CDS |
| TTTGTGTGAAAGTAGATTGA+TGG | 0.380197 | 6.3:+81428003 | MS.gene72251:CDS |
| ATGAAAAGTTCTGAAGCAAT+TGG | 0.388635 | 6.3:+81428530 | MS.gene72251:CDS |
| ATCACCATCTTTATCTTCAT+AGG | 0.403740 | 6.3:-81428056 | None:intergenic |
| TGATCTAGGGAACAAATTGT+AGG | 0.417413 | 6.3:+81427292 | None:intergenic |
| AGATTGATGGATCTCCTTCA+TGG | 0.440946 | 6.3:+81428016 | MS.gene72251:CDS |
| GTAGGTCAATTTGGCTCTCA+TGG | 0.457514 | 6.3:+81427962 | MS.gene72251:intron |
| TTCTTTCAGGAAGAATTCAA+TGG | 0.488415 | 6.3:+81427330 | MS.gene72251:CDS |
| ATGAAGATAAAGATGGTGAT+TGG | 0.490100 | 6.3:+81428059 | MS.gene72251:CDS |
| TATGTAAAAGTTAGCATGGA+TGG | 0.496315 | 6.3:+81427409 | MS.gene72251:CDS |
| TTGAATTCTTCCTGAAAGAA+CGG | 0.519997 | 6.3:-81427327 | None:intergenic |
| AAAATATGCACTTACTTCCA+TGG | 0.525031 | 6.3:-81428100 | None:intergenic |
| GATCCACTTTCCTCAAGTAT+GGG | 0.530733 | 6.3:-81427435 | None:intergenic |
| TTCCTGAAAGAACGGATTGG+TGG | 0.533390 | 6.3:-81427319 | None:intergenic |
| TGCTAGTTGGTGATGTTCCA+TGG | 0.551199 | 6.3:+81428083 | MS.gene72251:CDS |
| CCTTTATGTAAAAGTTAGCA+TGG | 0.552884 | 6.3:+81427405 | MS.gene72251:CDS |
| TTCTTCCTGAAAGAACGGAT+TGG | 0.577749 | 6.3:-81427322 | None:intergenic |
| CTCACCTATGAAGATAAAGA+TGG | 0.579438 | 6.3:+81428052 | MS.gene72251:CDS |
| TGCCCCATACTTGAGGAAAG+TGG | 0.579458 | 6.3:+81427432 | MS.gene72251:CDS |
| GATGGTGATTGGATGCTAGT+TGG | 0.601437 | 6.3:+81428070 | MS.gene72251:CDS |
| CATCTTTATCTTCATAGGTG+AGG | 0.607712 | 6.3:-81428051 | None:intergenic |
| GGACATATTCTGAACCATGA+AGG | 0.615918 | 6.3:-81428030 | None:intergenic |
| TGGATGGTGCCCCATACTTG+AGG | 0.620867 | 6.3:+81427425 | MS.gene72251:CDS |
| CTAGGGAACAAATTGTAGGA+TGG | 0.620947 | 6.3:+81427296 | None:intergenic |
| TGTTCATTGACTCTTGCAAG+AGG | 0.620974 | 6.3:+81428498 | MS.gene72251:CDS |
| ATCCACTTTCCTCAAGTATG+GGG | 0.656738 | 6.3:-81427434 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATATTATTCAAAAGTTTA+TGG | - | chr6.3:81428159-81428178 | None:intergenic | 15.0% |
| !! | ATTTGATTATTTGAAAGTTT+AGG | + | chr6.3:81428206-81428225 | MS.gene72251:intron | 15.0% |
| !!! | GTTTTTATTTTTGTTGTTTT+AGG | + | chr6.3:81428474-81428493 | MS.gene72251:intron | 15.0% |
| !! | ATGGAAATTATATTACGAAA+AGG | + | chr6.3:81428384-81428403 | MS.gene72251:intron | 20.0% |
| !! | TTAAAACGTACATAACTAAA+GGG | + | chr6.3:81427794-81427813 | MS.gene72251:intron | 20.0% |
| !! | TTTAAAGGAGTGATATTAAT+AGG | + | chr6.3:81427867-81427886 | MS.gene72251:intron | 20.0% |
| !!! | GTTATGTACGTTTTAAATAA+AGG | - | chr6.3:81427790-81427809 | None:intergenic | 20.0% |
| !!! | TTTAAAACGTACATAACTAA+AGG | + | chr6.3:81427793-81427812 | MS.gene72251:intron | 20.0% |
| !!! | TTTTTATATAGTTTTTGGAC+CGG | + | chr6.3:81428319-81428338 | MS.gene72251:intron | 20.0% |
| ! | ATAACGTTGTTGCAAATTAA+TGG | + | chr6.3:81428365-81428384 | MS.gene72251:intron | 25.0% |
| !! | AACAACTAAACATCTTTTCT+AGG | - | chr6.3:81427498-81427517 | None:intergenic | 25.0% |
| !! | ACAACTAAACATCTTTTCTA+GGG | - | chr6.3:81427497-81427516 | None:intergenic | 25.0% |
| !! | GCAACAACGTTATATATTTT+AGG | - | chr6.3:81428358-81428377 | None:intergenic | 25.0% |
| !! | TAGGTTTAAGATAACAATCT+CGG | + | chr6.3:81427578-81427597 | MS.gene72251:intron | 25.0% |
| !! | TTTAGGTTCAATTATTTAGC+CGG | - | chr6.3:81428341-81428360 | None:intergenic | 25.0% |
| !!! | AAAGTGGATCTCAAATTTTT+TGG | + | chr6.3:81427448-81427467 | MS.gene72251:CDS | 25.0% |
| !!! | TAGTACCGATTTTAGATTTA+AGG | + | chr6.3:81427668-81427687 | MS.gene72251:intron | 25.0% |
| !!! | TTTTGATGAATGAAAAGTGT+AGG | + | chr6.3:81427944-81427963 | MS.gene72251:intron | 25.0% |
| AAAATATGCACTTACTTCCA+TGG | - | chr6.3:81428103-81428122 | None:intergenic | 30.0% | |
| ACAACACTGATACAATGAAA+AGG | - | chr6.3:81427744-81427763 | None:intergenic | 30.0% | |
| ATCACCATCTTTATCTTCAT+AGG | - | chr6.3:81428059-81428078 | None:intergenic | 30.0% | |
| ATGAAGATAAAGATGGTGAT+TGG | + | chr6.3:81428059-81428078 | MS.gene72251:CDS | 30.0% | |
| ATTTGAAAACATCTCGATGA+CGG | - | chr6.3:81427716-81427735 | None:intergenic | 30.0% | |
| CCTTTATGTAAAAGTTAGCA+TGG | + | chr6.3:81427405-81427424 | MS.gene72251:CDS | 30.0% | |
| GTTGATATTGTGGAGAATTA+CGG | + | chr6.3:81427614-81427633 | MS.gene72251:intron | 30.0% | |
| TAAAAATCTCCTTAGCTCAA+TGG | - | chr6.3:81427851-81427870 | None:intergenic | 30.0% | |
| TATGTAAAAGTTAGCATGGA+TGG | + | chr6.3:81427409-81427428 | MS.gene72251:CDS | 30.0% | |
| TTCTTTCAGGAAGAATTCAA+TGG | + | chr6.3:81427330-81427349 | MS.gene72251:CDS | 30.0% | |
| TTGAATTCTTCCTGAAAGAA+CGG | - | chr6.3:81427330-81427349 | None:intergenic | 30.0% | |
| TTTGTGTGAAAGTAGATTGA+TGG | + | chr6.3:81428003-81428022 | MS.gene72251:CDS | 30.0% | |
| ! | ACACGCCTTAAATCTAAAAT+CGG | - | chr6.3:81427676-81427695 | None:intergenic | 30.0% |
| ! | AGGTTTAAGATAACAATCTC+GGG | + | chr6.3:81427579-81427598 | MS.gene72251:intron | 30.0% |
| ! | ATGAAAAGTGTAGGTCAATT+TGG | + | chr6.3:81427953-81427972 | MS.gene72251:intron | 30.0% |
| ! | CCATGCTAACTTTTACATAA+AGG | - | chr6.3:81427408-81427427 | None:intergenic | 30.0% |
| ! | TGAGCTAAGGAGATTTTTAA+AGG | + | chr6.3:81427852-81427871 | MS.gene72251:intron | 30.0% |
| !! | ATGAAAAGTTCTGAAGCAAT+TGG | + | chr6.3:81428530-81428549 | MS.gene72251:CDS | 30.0% |
| !! | TACATAAAGGCATCCAAATT+TGG | - | chr6.3:81427395-81427414 | None:intergenic | 30.0% |
| !!! | ATCATTCTTTTGAGATTGAG+TGG | - | chr6.3:81427356-81427375 | None:intergenic | 30.0% |
| !!! | CAAATTTTTTGGCACTTACA+AGG | + | chr6.3:81427459-81427478 | MS.gene72251:CDS | 30.0% |
| CATCTTTATCTTCATAGGTG+AGG | - | chr6.3:81428054-81428073 | None:intergenic | 35.0% | |
| CTCACCTATGAAGATAAAGA+TGG | + | chr6.3:81428052-81428071 | MS.gene72251:CDS | 35.0% | |
| GATGATGTAGAAGCCAAATT+TGG | + | chr6.3:81427379-81427398 | MS.gene72251:CDS | 35.0% | |
| GTACATAACTAAAGGGTAGT+TGG | + | chr6.3:81427801-81427820 | MS.gene72251:intron | 35.0% | |
| ! | AGTGCATATTTTGTCTCACA+TGG | + | chr6.3:81428111-81428130 | MS.gene72251:intron | 35.0% |
| ! | GTAGTTGGTTCATATGCTAA+GGG | + | chr6.3:81427816-81427835 | MS.gene72251:intron | 35.0% |
| !! | AGTTCTGAAGCAATTGGATT+AGG | + | chr6.3:81428536-81428555 | MS.gene72251:CDS | 35.0% |
| !! | GTTCTGAAGCAATTGGATTA+GGG | + | chr6.3:81428537-81428556 | MS.gene72251:CDS | 35.0% |
| AGATCCACTTTCCTCAAGTA+TGG | - | chr6.3:81427439-81427458 | None:intergenic | 40.0% | |
| ATCCACTTTCCTCAAGTATG+GGG | - | chr6.3:81427437-81427456 | None:intergenic | 40.0% | |
| CGTCATGATCGTTGATATTG+TGG | + | chr6.3:81427604-81427623 | MS.gene72251:intron | 40.0% | |
| GATCCACTTTCCTCAAGTAT+GGG | - | chr6.3:81427438-81427457 | None:intergenic | 40.0% | |
| GGACATATTCTGAACCATGA+AGG | - | chr6.3:81428033-81428052 | None:intergenic | 40.0% | |
| TGGAGAATTACGGTAAAACG+CGG | + | chr6.3:81427624-81427643 | MS.gene72251:intron | 40.0% | |
| TTCTTCCTGAAAGAACGGAT+TGG | - | chr6.3:81427325-81427344 | None:intergenic | 40.0% | |
| TTGACTCTTGCAAGAGGTTA+AGG | + | chr6.3:81428504-81428523 | MS.gene72251:CDS | 40.0% | |
| ! | AGATTGATGGATCTCCTTCA+TGG | + | chr6.3:81428016-81428035 | MS.gene72251:CDS | 40.0% |
| ! | GGTAGTTGGTTCATATGCTA+AGG | + | chr6.3:81427815-81427834 | MS.gene72251:intron | 40.0% |
| ! | TCTCATTTTGCTTGTGCACT+AGG | + | chr6.3:81427559-81427578 | MS.gene72251:intron | 40.0% |
| ! | TGGTTGATTCCATTGAGCTA+AGG | + | chr6.3:81427839-81427858 | MS.gene72251:intron | 40.0% |
| !! | TGTTCATTGACTCTTGCAAG+AGG | + | chr6.3:81428498-81428517 | MS.gene72251:CDS | 40.0% |
| !!! | TCTCATGGAGTTTTTGGTCA+AGG | + | chr6.3:81427977-81427996 | MS.gene72251:CDS | 40.0% |
| !!! | TTTGGCTCTCATGGAGTTTT+TGG | + | chr6.3:81427971-81427990 | MS.gene72251:CDS | 40.0% |
| AGTGTTGTGTCCGATGTTTG+TGG | + | chr6.3:81427756-81427775 | MS.gene72251:intron | 45.0% | |
| GATGGTGATTGGATGCTAGT+TGG | + | chr6.3:81428070-81428089 | MS.gene72251:CDS | 45.0% | |
| ! | GTTGGTTCATATGCTAAGGG+TGG | + | chr6.3:81427819-81427838 | MS.gene72251:intron | 45.0% |
| !! | GTAGGTCAATTTGGCTCTCA+TGG | + | chr6.3:81427962-81427981 | MS.gene72251:intron | 45.0% |
| !! | TGCTAGTTGGTGATGTTCCA+TGG | + | chr6.3:81428083-81428102 | MS.gene72251:CDS | 45.0% |
| !! | TTCCTGAAAGAACGGATTGG+TGG | - | chr6.3:81427322-81427341 | None:intergenic | 45.0% |
| !!! | AAATTTTTTTATATAGTTTT+TGG | + | chr6.3:81428314-81428333 | MS.gene72251:intron | 5.0% |
| GGCCTAACAGCCACAAACAT+CGG | - | chr6.3:81427769-81427788 | None:intergenic | 50.0% | |
| GTCCGATGTTTGTGGCTGTT+AGG | + | chr6.3:81427764-81427783 | MS.gene72251:intron | 50.0% | |
| TGCCCCATACTTGAGGAAAG+TGG | + | chr6.3:81427432-81427451 | MS.gene72251:CDS | 50.0% | |
| GGCCACCAATCCGTTCTTTC+AGG | + | chr6.3:81427317-81427336 | MS.gene72251:CDS | 55.0% | |
| TGGATGGTGCCCCATACTTG+AGG | + | chr6.3:81427425-81427444 | MS.gene72251:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 81427300 | 81428557 | 81427300 | ID=MS.gene72251 |
| chr6.3 | mRNA | 81427300 | 81428557 | 81427300 | ID=MS.gene72251.t1;Parent=MS.gene72251 |
| chr6.3 | exon | 81427300 | 81427526 | 81427300 | ID=MS.gene72251.t1.exon1;Parent=MS.gene72251.t1 |
| chr6.3 | CDS | 81427300 | 81427526 | 81427300 | ID=cds.MS.gene72251.t1;Parent=MS.gene72251.t1 |
| chr6.3 | exon | 81427966 | 81428107 | 81427966 | ID=MS.gene72251.t1.exon2;Parent=MS.gene72251.t1 |
| chr6.3 | CDS | 81427966 | 81428107 | 81427966 | ID=cds.MS.gene72251.t1;Parent=MS.gene72251.t1 |
| chr6.3 | exon | 81428496 | 81428557 | 81428496 | ID=MS.gene72251.t1.exon3;Parent=MS.gene72251.t1 |
| chr6.3 | CDS | 81428496 | 81428557 | 81428496 | ID=cds.MS.gene72251.t1;Parent=MS.gene72251.t1 |
| Gene Sequence |
| Protein sequence |
