Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72401.t1 | KEH31643.1 | 96.9 | 98 | 3 | 0 | 1 | 98 | 1 | 98 | 9.30E-40 | 172.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72401.t1 | A0A072URE5 | 96.9 | 98 | 3 | 0 | 1 | 98 | 1 | 98 | 6.7e-40 | 172.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060483 | MS.gene72401 | 0.808506 | 3.00E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72401.t1 | MTR_4g100820 | 96.939 | 98 | 3 | 0 | 1 | 98 | 1 | 98 | 4.75e-61 | 181 |
| MS.gene72401.t1 | MTR_4g100820 | 96.667 | 90 | 3 | 0 | 1 | 90 | 1 | 90 | 7.97e-55 | 165 |
| MS.gene72401.t1 | MTR_4g100820 | 94.505 | 91 | 5 | 0 | 1 | 91 | 1 | 91 | 3.08e-54 | 164 |
| MS.gene72401.t1 | MTR_4g100820 | 96.591 | 88 | 3 | 0 | 1 | 88 | 1 | 88 | 6.13e-53 | 160 |
| MS.gene72401.t1 | MTR_3g109720 | 46.250 | 80 | 43 | 0 | 19 | 98 | 14 | 93 | 6.53e-15 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 55.556 | 99 | 43 | 1 | 1 | 98 | 1 | 99 | 1.12e-24 | 89.4 |
| MS.gene72401.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene72401.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene72401.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene72401.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene72401.t1 | AT4G17310 | 52.747 | 91 | 42 | 1 | 1 | 90 | 1 | 91 | 5.20e-19 | 75.1 |
| MS.gene72401.t1 | AT5G47455 | 68.333 | 60 | 19 | 0 | 39 | 98 | 49 | 108 | 5.53e-19 | 75.5 |
| MS.gene72401.t1 | AT5G47455 | 68.333 | 60 | 19 | 0 | 39 | 98 | 49 | 108 | 5.53e-19 | 75.5 |
| MS.gene72401.t1 | AT5G47455 | 68.333 | 60 | 19 | 0 | 39 | 98 | 49 | 108 | 5.53e-19 | 75.5 |
| MS.gene72401.t1 | AT4G17310 | 51.685 | 89 | 42 | 1 | 1 | 88 | 1 | 89 | 2.43e-17 | 70.9 |
| MS.gene72401.t1 | AT4G17310 | 51.685 | 89 | 42 | 1 | 1 | 88 | 1 | 89 | 4.22e-17 | 70.1 |
| MS.gene72401.t1 | AT5G47455 | 65.385 | 52 | 18 | 0 | 39 | 90 | 49 | 100 | 2.70e-13 | 60.5 |
| MS.gene72401.t1 | AT5G47455 | 65.385 | 52 | 18 | 0 | 39 | 90 | 49 | 100 | 2.70e-13 | 60.5 |
| MS.gene72401.t1 | AT5G47455 | 65.385 | 52 | 18 | 0 | 39 | 90 | 49 | 100 | 2.70e-13 | 60.5 |
| MS.gene72401.t1 | AT5G47455 | 65.385 | 52 | 18 | 0 | 39 | 90 | 49 | 100 | 2.70e-13 | 60.5 |
| MS.gene72401.t1 | AT5G11630 | 41.414 | 99 | 51 | 4 | 1 | 98 | 1 | 93 | 4.99e-13 | 59.7 |
| MS.gene72401.t1 | AT5G11630 | 41.414 | 99 | 51 | 4 | 1 | 98 | 1 | 93 | 4.99e-13 | 59.7 |
| MS.gene72401.t1 | AT5G47455 | 62.963 | 54 | 19 | 1 | 39 | 91 | 49 | 102 | 8.11e-12 | 57.4 |
| MS.gene72401.t1 | AT5G47455 | 60.377 | 53 | 21 | 0 | 39 | 91 | 49 | 101 | 9.56e-12 | 56.6 |
| MS.gene72401.t1 | AT5G47455 | 60.377 | 53 | 21 | 0 | 39 | 91 | 49 | 101 | 9.56e-12 | 56.6 |
| MS.gene72401.t1 | AT5G47455 | 64.000 | 50 | 18 | 0 | 39 | 88 | 49 | 98 | 1.66e-11 | 56.2 |
Find 28 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCAGTGGTGGTTGGTTT+GGG | 0.163477 | 8.3:-20308493 | None:intergenic |
| TCCAAGCTCCGGCGCAATTC+TGG | 0.241996 | 8.3:-20308545 | None:intergenic |
| TGTGGCAGTGGTGGTTGGTT+TGG | 0.246581 | 8.3:-20308494 | None:intergenic |
| GGTTGGTTTGGGGATGGATG+AGG | 0.393701 | 8.3:-20308482 | None:intergenic |
| GGATTTAATTGCGGATTTGA+TGG | 0.409263 | 8.3:-20308449 | None:intergenic |
| AGTGGTGGTTGGTTTGGGGA+TGG | 0.417037 | 8.3:-20308488 | None:intergenic |
| TCCAGAATTGCGCCGGAGCT+TGG | 0.422852 | 8.3:+20308544 | MS.gene72401:CDS |
| CACGTCTCAGCGTAACTTCG+AGG | 0.434582 | 8.3:+20308623 | MS.gene72401:CDS |
| GATGGATGAGGTGCGAATGT+TGG | 0.451724 | 8.3:-20308470 | None:intergenic |
| GACTGCACGCATCCAAGCTC+CGG | 0.453744 | 8.3:-20308556 | None:intergenic |
| CTGCTGCATGCGCAAGCCAT+TGG | 0.469294 | 8.3:-20308400 | None:intergenic |
| TGGCAGTGGTGGTTGGTTTG+GGG | 0.472598 | 8.3:-20308492 | None:intergenic |
| GCCGCAACTGCGCTGTGAAG+TGG | 0.483273 | 8.3:-20308586 | None:intergenic |
| ACTCTCTGCTGCACCTCTCC+CGG | 0.490651 | 8.3:+20308670 | MS.gene72401:CDS |
| GAAGATGTGGCAGTGGTGGT+TGG | 0.504244 | 8.3:-20308499 | None:intergenic |
| GAACTGTCAATCGCTTTCAC+AGG | 0.505088 | 8.3:+20308645 | MS.gene72401:CDS |
| GCCACTTCACAGCGCAGTTG+CGG | 0.509854 | 8.3:+20308585 | MS.gene72401:CDS |
| AAAAGAAAACATTAGAGTCC+GGG | 0.554992 | 8.3:-20308688 | None:intergenic |
| ATTTGCATCCAGAATTGCGC+CGG | 0.566247 | 8.3:+20308537 | MS.gene72401:CDS |
| GCGAATGTTGGATTTAATTG+CGG | 0.569994 | 8.3:-20308458 | None:intergenic |
| TGGAGAAGATGTGGCAGTGG+TGG | 0.576473 | 8.3:-20308503 | None:intergenic |
| CAATTCTGGATGCAAATGAT+CGG | 0.592552 | 8.3:-20308531 | None:intergenic |
| AAAACATTAGAGTCCGGGAG+AGG | 0.598769 | 8.3:-20308683 | None:intergenic |
| GATGCAAATGATCGGCGAAG+TGG | 0.621136 | 8.3:-20308523 | None:intergenic |
| AACTGTCAATCGCTTTCACA+GGG | 0.623914 | 8.3:+20308646 | MS.gene72401:CDS |
| ACTTCACAGCGCAGTTGCGG+CGG | 0.632388 | 8.3:+20308588 | MS.gene72401:CDS |
| TCGGCGAAGTGGAGAAGATG+TGG | 0.637549 | 8.3:-20308512 | None:intergenic |
| AAGTGGAGAAGATGTGGCAG+TGG | 0.639588 | 8.3:-20308506 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAATTCTGGATGCAAATGAT+CGG | - | chr8.3:20308534-20308553 | None:intergenic | 35.0% | |
| GCGAATGTTGGATTTAATTG+CGG | - | chr8.3:20308461-20308480 | None:intergenic | 35.0% | |
| GGATTTAATTGCGGATTTGA+TGG | - | chr8.3:20308452-20308471 | None:intergenic | 35.0% | |
| AACTGTCAATCGCTTTCACA+GGG | + | chr8.3:20308646-20308665 | MS.gene72401:CDS | 40.0% | |
| ATTTGCATCCAGAATTGCGC+CGG | + | chr8.3:20308537-20308556 | MS.gene72401:CDS | 45.0% | |
| GAACTGTCAATCGCTTTCAC+AGG | + | chr8.3:20308645-20308664 | MS.gene72401:CDS | 45.0% | |
| AAGTGGAGAAGATGTGGCAG+TGG | - | chr8.3:20308509-20308528 | None:intergenic | 50.0% | |
| GATGCAAATGATCGGCGAAG+TGG | - | chr8.3:20308526-20308545 | None:intergenic | 50.0% | |
| GATGGATGAGGTGCGAATGT+TGG | - | chr8.3:20308473-20308492 | None:intergenic | 50.0% | |
| CACGTCTCAGCGTAACTTCG+AGG | + | chr8.3:20308623-20308642 | MS.gene72401:CDS | 55.0% | |
| GAAGATGTGGCAGTGGTGGT+TGG | - | chr8.3:20308502-20308521 | None:intergenic | 55.0% | |
| TCGGCGAAGTGGAGAAGATG+TGG | - | chr8.3:20308515-20308534 | None:intergenic | 55.0% | |
| TGGAGAAGATGTGGCAGTGG+TGG | - | chr8.3:20308506-20308525 | None:intergenic | 55.0% | |
| ! | TGTGGCAGTGGTGGTTGGTT+TGG | - | chr8.3:20308497-20308516 | None:intergenic | 55.0% |
| !! | AGTGGTGGTTGGTTTGGGGA+TGG | - | chr8.3:20308491-20308510 | None:intergenic | 55.0% |
| !! | GGTTGGTTTGGGGATGGATG+AGG | - | chr8.3:20308485-20308504 | None:intergenic | 55.0% |
| !! | GTGGCAGTGGTGGTTGGTTT+GGG | - | chr8.3:20308496-20308515 | None:intergenic | 55.0% |
| !! | TGGCAGTGGTGGTTGGTTTG+GGG | - | chr8.3:20308495-20308514 | None:intergenic | 55.0% |
| ACTCTCTGCTGCACCTCTCC+CGG | + | chr8.3:20308670-20308689 | MS.gene72401:CDS | 60.0% | |
| ACTTCACAGCGCAGTTGCGG+CGG | + | chr8.3:20308588-20308607 | MS.gene72401:CDS | 60.0% | |
| GACTGCACGCATCCAAGCTC+CGG | - | chr8.3:20308559-20308578 | None:intergenic | 60.0% | |
| GCCACTTCACAGCGCAGTTG+CGG | + | chr8.3:20308585-20308604 | MS.gene72401:CDS | 60.0% | |
| TCCAAGCTCCGGCGCAATTC+TGG | - | chr8.3:20308548-20308567 | None:intergenic | 60.0% | |
| TCCAGAATTGCGCCGGAGCT+TGG | + | chr8.3:20308544-20308563 | MS.gene72401:CDS | 60.0% | |
| GCCGCAACTGCGCTGTGAAG+TGG | - | chr8.3:20308589-20308608 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 20308403 | 20308699 | 20308403 | ID=MS.gene72401 |
| chr8.3 | mRNA | 20308403 | 20308699 | 20308403 | ID=MS.gene72401.t1;Parent=MS.gene72401 |
| chr8.3 | exon | 20308403 | 20308699 | 20308403 | ID=MS.gene72401.t1.exon1;Parent=MS.gene72401.t1 |
| chr8.3 | CDS | 20308403 | 20308699 | 20308403 | ID=cds.MS.gene72401.t1;Parent=MS.gene72401.t1 |
| Gene Sequence |
| Protein sequence |
