AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060483


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060483.t1 MTR_8g024350 98.148 108 2 0 1 108 1 108 1.26e-72 211
MS.gene060483.t1 MTR_3g092600 60.000 110 42 1 1 108 1 110 4.73e-29 101
MS.gene060483.t1 MTR_2g066750 60.909 110 41 1 1 108 1 110 4.73e-29 101
MS.gene060483.t1 MTR_7g118060 66.102 59 20 0 1 59 1 59 1.53e-23 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060483.t1 AT5G24510 57.391 115 38 4 1 108 1 111 1.06e-37 123
MS.gene060483.t1 AT1G01100 51.402 107 39 1 2 108 3 96 7.04e-32 108
MS.gene060483.t1 AT4G00810 53.153 111 48 1 2 108 3 113 2.94e-31 107
MS.gene060483.t1 AT4G00810 53.153 111 48 1 2 108 3 113 2.94e-31 107
MS.gene060483.t1 AT1G01100 53.636 110 48 1 2 108 3 112 6.42e-31 106
MS.gene060483.t1 AT1G01100 53.636 110 48 1 2 108 3 112 6.42e-31 106
MS.gene060483.t1 AT1G01100 53.636 110 48 1 2 108 3 112 6.42e-31 106
MS.gene060483.t1 AT5G47700 53.153 111 48 1 2 108 3 113 9.37e-31 105
MS.gene060483.t1 AT5G47700 53.153 111 48 1 2 108 3 113 9.37e-31 105

Find 28 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAGGCAGCAGCAGCAAC+TGG 0.216487 8.1:-75971569 None:intergenic
ACGTTAATGTTGAAGCTTAC+TGG 0.234944 8.1:+75971453 MS.gene060483:CDS
TGGTGCTCCGGTTTCTGTTT+CGG 0.321080 8.1:+75971544 MS.gene060483:CDS
GTCGTCGTCACTCTCATCCT+TGG 0.357062 8.1:-75971719 None:intergenic
ATCATCAAGGTTCTTCTTCT+CGG 0.364681 8.1:-75971498 None:intergenic
ACTGGGGCCGAAACAGAAAC+CGG 0.462684 8.1:-75971551 None:intergenic
TCGTCGTCACTCTCATCCTT+GGG 0.464875 8.1:-75971718 None:intergenic
TGCCGCTGGAGGTGGTGCTC+CGG 0.491299 8.1:+75971532 MS.gene060483:CDS
ATATTGAATGCCGCTGGAGG+TGG 0.496313 8.1:+75971524 MS.gene060483:CDS
GCAAGTTTAGCGAATAAACT+TGG 0.520693 8.1:-75971476 None:intergenic
TGCTCCTATCGAGGAGAAGA+AGG 0.520970 8.1:+75971601 MS.gene060483:CDS
TGGAGGCAGCAGCAGCAACT+GGG 0.544694 8.1:-75971568 None:intergenic
AGGAGCAGCAACTGCAGTGG+AGG 0.545209 8.1:-75971585 None:intergenic
GGATGAGAGTGACGACGACA+TGG 0.547177 8.1:+75971723 MS.gene060483:CDS
GTTTGCAGCTTTCAACAAAG+TGG 0.560297 8.1:-75971432 None:intergenic
GATTTGATATTGAATGCCGC+TGG 0.580711 8.1:+75971518 MS.gene060483:CDS
AACCGGAGCACCACCTCCAG+CGG 0.583475 8.1:-75971534 None:intergenic
CGGCGATGTTATCATCATGA+AGG 0.588233 8.1:-75970729 None:intergenic
TGTAGTGTGAAAATGTCGAT+CGG 0.589603 8.1:+75970676 None:intergenic
CATTCAATATCAAATCATCA+AGG 0.592309 8.1:-75971511 None:intergenic
TGCAGTTGCTGCTCCTATCG+AGG 0.607768 8.1:+75971592 MS.gene060483:CDS
GGAGGCAGCAGCAGCAACTG+GGG 0.621755 8.1:-75971567 None:intergenic
CCTACCTTCTTCTCCTCGAT+AGG 0.622145 8.1:-75971605 None:intergenic
GATAGGAGCAGCAACTGCAG+TGG 0.632714 8.1:-75971588 None:intergenic
ATTATCAAAGGATACAGTGA+CGG 0.653291 8.1:-75970749 None:intergenic
GATGAGAGTGACGACGACAT+GGG 0.653294 8.1:+75971724 MS.gene060483:CDS
GTACTTACAGGAAGAACCCA+AGG 0.654187 8.1:+75971702 MS.gene060483:intron
TTGATATTGAATGCCGCTGG+AGG 0.659375 8.1:+75971521 MS.gene060483:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATCAATTTTTATATAGT+GGG + chr8.1:75971277-75971296 MS.gene060483:intron 10.0%
!!! TAAAAAATTAGGTTTTTTAA+AGG + chr8.1:75971125-75971144 MS.gene060483:intron 10.0%
!!! TAAAATCAATTTTTATATAG+TGG + chr8.1:75971276-75971295 MS.gene060483:intron 10.0%
!!! TTAAAAAACCTAATTTTTTA+AGG - chr8.1:75971125-75971144 None:intergenic 10.0%
!! AAAAATTCAGTAATAATTTG+AGG - chr8.1:75970993-75971012 None:intergenic 15.0%
!! AAATTAGTCCTTAAAAAATT+AGG + chr8.1:75971114-75971133 MS.gene060483:intron 15.0%
!! ATGAATTAATCAAATGTAAT+TGG - chr8.1:75970968-75970987 None:intergenic 15.0%
!! ATTACATTATCAATTATCAA+AGG - chr8.1:75970764-75970783 None:intergenic 15.0%
!!! AAAAATTGATTTTACAACTT+TGG - chr8.1:75971270-75971289 None:intergenic 15.0%
!!! ACTAAATTAACAATTTTCTA+CGG + chr8.1:75971237-75971256 MS.gene060483:intron 15.0%
!!! ATTATTACTGAATTTTTTAC+TGG + chr8.1:75970996-75971015 MS.gene060483:intron 15.0%
!!! TTGAAAAAAAGAGATTTTTT+TGG + chr8.1:75970856-75970875 MS.gene060483:intron 15.0%
!! AAATTCAGAATATAATTCCT+AGG + chr8.1:75971054-75971073 MS.gene060483:intron 20.0%
!!! GATTTTCTTTTGTTAGTATA+TGG + chr8.1:75970796-75970815 MS.gene060483:intron 20.0%
!!! TCTTATCTAATTGGTATTTT+TGG + chr8.1:75971677-75971696 MS.gene060483:intron 20.0%
! CATTCAATATCAAATCATCA+AGG - chr8.1:75971514-75971533 None:intergenic 25.0%
! TAATGAATTTAGAAGATGCT+AGG + chr8.1:75970824-75970843 MS.gene060483:intron 25.0%
!! ATTAACAATTTTCTACGGTT+AGG + chr8.1:75971242-75971261 MS.gene060483:intron 25.0%
!! TTAACAATTTTCTACGGTTA+GGG + chr8.1:75971243-75971262 MS.gene060483:intron 25.0%
!!! AGATTTTTTTGGATGATACA+AGG + chr8.1:75970867-75970886 MS.gene060483:intron 25.0%
!!! ATTTTTATATAGTGGGAATC+TGG + chr8.1:75971284-75971303 MS.gene060483:intron 25.0%
!!! ATTTTTTTGGATGATACAAG+GGG + chr8.1:75970869-75970888 MS.gene060483:intron 25.0%
!!! GAATTTTGTTTTGGTGAATA+AGG + chr8.1:75971312-75971331 MS.gene060483:intron 25.0%
!!! GATTTTTTTGGATGATACAA+GGG + chr8.1:75970868-75970887 MS.gene060483:intron 25.0%
!!! TTTTTATATAGTGGGAATCT+GGG + chr8.1:75971285-75971304 MS.gene060483:intron 25.0%
AATGACAATTAACAACTCCT+AGG - chr8.1:75971074-75971093 None:intergenic 30.0%
ATTATCAAAGGATACAGTGA+CGG - chr8.1:75970752-75970771 None:intergenic 30.0%
ATTTAGAAGATGCTAGGAAT+TGG + chr8.1:75970830-75970849 MS.gene060483:intron 30.0%
TAAAGTTCTTGGATAGAGTT+AGG + chr8.1:75971174-75971193 MS.gene060483:intron 30.0%
TAAGTGCTAGTTAAAGTTCT+TGG + chr8.1:75971163-75971182 MS.gene060483:intron 30.0%
TCCTTAAGTCATTGTTGATA+GGG + chr8.1:75971398-75971417 MS.gene060483:intron 30.0%
TGTTGGTCATCTTATCTAAT+TGG + chr8.1:75971668-75971687 MS.gene060483:intron 30.0%
TTTAGAAGATGCTAGGAATT+GGG + chr8.1:75970831-75970850 MS.gene060483:intron 30.0%
!!! AGATAGCGTGTTTTAAAGAT+TGG + chr8.1:75971371-75971390 MS.gene060483:intron 30.0%
!!! CTGGGTTTTGAATTTTGTTT+TGG + chr8.1:75971303-75971322 MS.gene060483:intron 30.0%
!!! GTATTTTTGGTTGTACTTAC+AGG + chr8.1:75971690-75971709 MS.gene060483:intron 30.0%
ACCCTATCAACAATGACTTA+AGG - chr8.1:75971402-75971421 None:intergenic 35.0%
ACGTTAATGTTGAAGCTTAC+TGG + chr8.1:75971453-75971472 MS.gene060483:CDS 35.0%
ATCATCAAGGTTCTTCTTCT+CGG - chr8.1:75971501-75971520 None:intergenic 35.0%
CTCCTTAAGTCATTGTTGAT+AGG + chr8.1:75971397-75971416 MS.gene060483:intron 35.0%
GAACTTTGTCTTCTGTTTGT+TGG + chr8.1:75971651-75971670 MS.gene060483:intron 35.0%
GCAAGTTTAGCGAATAAACT+TGG - chr8.1:75971479-75971498 None:intergenic 35.0%
TTTGGTGAATAAGGAAAGTG+TGG + chr8.1:75971321-75971340 MS.gene060483:intron 35.0%
! AATAAGGAAAGTGTGGTGTA+AGG + chr8.1:75971328-75971347 MS.gene060483:intron 35.0%
! ATAAGGAAAGTGTGGTGTAA+GGG + chr8.1:75971329-75971348 MS.gene060483:intron 35.0%
GATGATACAAGGGGCTATAT+AGG + chr8.1:75970878-75970897 MS.gene060483:intron 40.0%
GATTTGATATTGAATGCCGC+TGG + chr8.1:75971518-75971537 MS.gene060483:CDS 40.0%
GTTTGCAGCTTTCAACAAAG+TGG - chr8.1:75971435-75971454 None:intergenic 40.0%
! AGGAGAAGAAGGTAGGTTTT+TGG + chr8.1:75971612-75971631 MS.gene060483:intron 40.0%
CCTAAAACGACACTTGTGTC+TGG + chr8.1:75970909-75970928 MS.gene060483:intron 45.0%
CGGCGATGTTATCATCATGA+AGG - chr8.1:75970732-75970751 None:intergenic 45.0%
GTACTTACAGGAAGAACCCA+AGG + chr8.1:75971702-75971721 MS.gene060483:intron 45.0%
TTGATATTGAATGCCGCTGG+AGG + chr8.1:75971521-75971540 MS.gene060483:CDS 45.0%
!!! CCAGACACAAGTGTCGTTTT+AGG - chr8.1:75970912-75970931 None:intergenic 45.0%
!!! TTTTTTTATTTTCTTATTTT+AGG + chr8.1:75971214-75971233 MS.gene060483:intron 5.0%
ATATTGAATGCCGCTGGAGG+TGG + chr8.1:75971524-75971543 MS.gene060483:CDS 50.0%
CCTACCTTCTTCTCCTCGAT+AGG - chr8.1:75971608-75971627 None:intergenic 50.0%
CCTATCGAGGAGAAGAAGGT+AGG + chr8.1:75971605-75971624 MS.gene060483:intron 50.0%
GATGAGAGTGACGACGACAT+GGG + chr8.1:75971724-75971743 MS.gene060483:CDS 50.0%
TCGTCGTCACTCTCATCCTT+GGG - chr8.1:75971721-75971740 None:intergenic 50.0%
TGCTCCTATCGAGGAGAAGA+AGG + chr8.1:75971601-75971620 MS.gene060483:CDS 50.0%
TGGTGCTCCGGTTTCTGTTT+CGG + chr8.1:75971544-75971563 MS.gene060483:CDS 50.0%
TGGTGTGTGTCAGTGCTTCA+TGG + chr8.1:75971016-75971035 MS.gene060483:intron 50.0%
ACTGGGGCCGAAACAGAAAC+CGG - chr8.1:75971554-75971573 None:intergenic 55.0%
GATAGGAGCAGCAACTGCAG+TGG - chr8.1:75971591-75971610 None:intergenic 55.0%
GGATGAGAGTGACGACGACA+TGG + chr8.1:75971723-75971742 MS.gene060483:CDS 55.0%
GTCGTCGTCACTCTCATCCT+TGG - chr8.1:75971722-75971741 None:intergenic 55.0%
TGCAGTTGCTGCTCCTATCG+AGG + chr8.1:75971592-75971611 MS.gene060483:CDS 55.0%
AGGAGCAGCAACTGCAGTGG+AGG - chr8.1:75971588-75971607 None:intergenic 60.0%
TGGAGGCAGCAGCAGCAACT+GGG - chr8.1:75971571-75971590 None:intergenic 60.0%
GGAGGCAGCAGCAGCAACTG+GGG - chr8.1:75971570-75971589 None:intergenic 65.0%
GTGGAGGCAGCAGCAGCAAC+TGG - chr8.1:75971572-75971591 None:intergenic 65.0%
! AACCGGAGCACCACCTCCAG+CGG - chr8.1:75971537-75971556 None:intergenic 65.0%
TGCCGCTGGAGGTGGTGCTC+CGG + chr8.1:75971532-75971551 MS.gene060483:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 75970688 75971765 75970688 ID=MS.gene060483
chr8.1 mRNA 75970688 75971765 75970688 ID=MS.gene060483.t1;Parent=MS.gene060483
chr8.1 exon 75970688 75970756 75970688 ID=MS.gene060483.t1.exon1;Parent=MS.gene060483.t1
chr8.1 CDS 75970688 75970756 75970688 ID=cds.MS.gene060483.t1;Parent=MS.gene060483.t1
chr8.1 exon 75971419 75971622 75971419 ID=MS.gene060483.t1.exon2;Parent=MS.gene060483.t1
chr8.1 CDS 75971419 75971622 75971419 ID=cds.MS.gene060483.t1;Parent=MS.gene060483.t1
chr8.1 exon 75971712 75971765 75971712 ID=MS.gene060483.t1.exon3;Parent=MS.gene060483.t1
chr8.1 CDS 75971712 75971765 75971712 ID=cds.MS.gene060483.t1;Parent=MS.gene060483.t1
Gene Sequence

>MS.gene060483

ATGTCGATCGGAGAAGTTGCTTGCAGCTACTCACTTATGATCCTTCATGATGATAACATCGCCGTCACTGTATCCTTTGATAATTGATAATGTAATTAATTTAATTTTGATTTTCTTTTGTTAGTATATGGTTTTTTAATGAATTTAGAAGATGCTAGGAATTGGGTTTTGAAAAAAAGAGATTTTTTTGGATGATACAAGGGGCTATATAGGATTATGAACCTAAAACGACACTTGTGTCTGGTGTCTTTTGCGTGTTTTGTCAGCGCACTCACATCCAATTACATTTGATTAATTCATTTCCTCAAATTATTACTGAATTTTTTACTGGTGTGTGTCAGTGCTTCATGGTCTCTGATGCAAGTGAAATTCAGAATATAATTCCTAGGAGTTGTTAATTGTCATTTAGATTCAATTATTGAAATCAAATTAGTCCTTAAAAAATTAGGTTTTTTAAAGGTTAAACTAGTTTTGATAAGTGCTAGTTAAAGTTCTTGGATAGAGTTAGGATAATGTTTTTGTGATTTTTTTTTATTTTCTTATTTTAGGACTAAATTAACAATTTTCTACGGTTAGGGACCAAAGTTGTAAAATCAATTTTTATATAGTGGGAATCTGGGTTTTGAATTTTGTTTTGGTGAATAAGGAAAGTGTGGTGTAAGGGTTTGAAGTTGAATATTTGAAGATAGCGTGTTTTAAAGATTGGTTTCTCCTTAAGTCATTGTTGATAGGGTGAGAAAATCTCCACTTTGTTGAAAGCTGCAAACGTTAATGTTGAAGCTTACTGGCCAAGTTTATTCGCTAAACTTGCCGAGAAGAAGAACCTTGATGATTTGATATTGAATGCCGCTGGAGGTGGTGCTCCGGTTTCTGTTTCGGCCCCAGTTGCTGCTGCTGCCTCCACTGCAGTTGCTGCTCCTATCGAGGAGAAGAAGGTAGGTTTTTGGTGAAAAAGTTGTTGTTGAACTTTGTCTTCTGTTTGTTGGTCATCTTATCTAATTGGTATTTTTGGTTGTACTTACAGGAAGAACCCAAGGATGAGAGTGACGACGACATGGGATTCAGCTTGTTTGATTAG

Protein sequence

>MS.gene060483.t1

MSIGEVACSYSLMILHDDNIAVTGEKISTLLKAANVNVEAYWPSLFAKLAEKKNLDDLILNAAGGGAPVSVSAPVAAAASTAVAAPIEEKKEEPKDESDDDMGFSLFD