Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72422.t1 | XP_003624491.1 | 84.5 | 193 | 30 | 0 | 1 | 193 | 1 | 193 | 1.00E-61 | 246.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72422.t1 | B7FGN6 | 84.5 | 193 | 30 | 0 | 1 | 193 | 1 | 193 | 7.2e-62 | 246.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050252 | MS.gene72422 | 0.807819 | 4.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72422.t1 | MTR_7g083930 | 97.409 | 193 | 5 | 0 | 1 | 193 | 1 | 193 | 1.02e-136 | 380 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72422.t1 | AT2G31410 | 60.417 | 192 | 72 | 3 | 1 | 191 | 1 | 189 | 1.91e-61 | 190 |
Find 61 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAAGTCTCCGGCAGAAAA+TGG | 0.278662 | 7.4:-23195685 | MS.gene72422:CDS |
| TCTTGGCATCTTCGATTGCT+TGG | 0.321144 | 7.4:+23195852 | None:intergenic |
| CTTGGCATCTTCGATTGCTT+GGG | 0.352355 | 7.4:+23195853 | None:intergenic |
| TCGTCGCGCTTCAGCTCTTC+AGG | 0.356562 | 7.4:-23195648 | MS.gene72422:CDS |
| CGATGACGCCGTCGTAGGTT+GGG | 0.366226 | 7.4:+23195711 | None:intergenic |
| CTAAGCAGAGGAAGCAGCTT+AGG | 0.368607 | 7.4:-23195400 | MS.gene72422:CDS |
| GCGATGACGCCGTCGTAGGT+TGG | 0.373346 | 7.4:+23195710 | None:intergenic |
| ACCTTCTCCGCAGTCTCCTT+CGG | 0.373542 | 7.4:-23195737 | MS.gene72422:CDS |
| GCGGAGAAGGTGGGTTTGTT+GGG | 0.393797 | 7.4:+23195749 | None:intergenic |
| AGAGGCGATAAGGATGAATA+AGG | 0.399865 | 7.4:-23195540 | MS.gene72422:CDS |
| TGCGGAGAAGGTGGGTTTGT+TGG | 0.406363 | 7.4:+23195748 | None:intergenic |
| CGTCACCTCGATGAAGGCTT+CGG | 0.429926 | 7.4:-23195908 | MS.gene72422:CDS |
| TTGTTCGCGTTTGCGTTTGT+AGG | 0.430764 | 7.4:+23195877 | None:intergenic |
| GGGTTTGTTGGGATCTGATA+AGG | 0.430834 | 7.4:+23195760 | None:intergenic |
| TATTTCTTAATCATATCATC+AGG | 0.441029 | 7.4:+23195371 | None:intergenic |
| AGCGAGAGAGAAGAGCATTA+AGG | 0.441499 | 7.4:-23195594 | MS.gene72422:CDS |
| GATTTCCGTCACCTCGATGA+AGG | 0.444455 | 7.4:-23195914 | MS.gene72422:CDS |
| AGAGAGTGAGAAGAGGAAGA+AGG | 0.465303 | 7.4:-23195495 | MS.gene72422:CDS |
| GGATACGATTTCTTCCATCT+TGG | 0.468088 | 7.4:+23195835 | None:intergenic |
| CGGAGTTGAAAGAGGCGATA+AGG | 0.468478 | 7.4:-23195550 | MS.gene72422:CDS |
| GTCATCGCCGGCAAAGTCTC+CGG | 0.471584 | 7.4:-23195695 | MS.gene72422:CDS |
| GGGAGGGCAGATCGCTTGGC+TGG | 0.481590 | 7.4:+23195785 | None:intergenic |
| CAAGAAGGGAACGACGTTTG+AGG | 0.484378 | 7.4:-23195621 | MS.gene72422:CDS |
| TGAATAAGGTGGAGAAGAGA+AGG | 0.492464 | 7.4:-23195526 | MS.gene72422:CDS |
| GGATGGGAGGGCAGATCGCT+TGG | 0.492944 | 7.4:+23195781 | None:intergenic |
| AGGGCAGATCGCTTGGCTGG+TGG | 0.501875 | 7.4:+23195788 | None:intergenic |
| TGCGGTGTCGTCGGTTTCCA+TGG | 0.504766 | 7.4:+23195814 | None:intergenic |
| ACCGAAGGAGACTGCGGAGA+AGG | 0.506049 | 7.4:+23195736 | None:intergenic |
| GCTCTTCAGGTGAGCAAGAA+GGG | 0.507561 | 7.4:-23195635 | MS.gene72422:CDS |
| GATGGAAGAAATCGTATCCA+TGG | 0.511500 | 7.4:-23195831 | MS.gene72422:CDS |
| AGAGAGAAGAGAGTGAGAAG+AGG | 0.518204 | 7.4:-23195502 | MS.gene72422:CDS |
| ACCTACGACGGCGTCATCGC+CGG | 0.519391 | 7.4:-23195707 | MS.gene72422:CDS |
| AGCTCTTCAGGTGAGCAAGA+AGG | 0.525692 | 7.4:-23195636 | MS.gene72422:CDS |
| GATGACGCCGTCGTAGGTTG+GGG | 0.532347 | 7.4:+23195712 | None:intergenic |
| GAAATCGATGGTGCAGGCCA+TGG | 0.533089 | 7.4:+23195931 | None:intergenic |
| GTGACGGAAATCGATGGTGC+AGG | 0.542066 | 7.4:+23195925 | None:intergenic |
| GTCGTAGGTTGGGGCACCGA+AGG | 0.543026 | 7.4:+23195721 | None:intergenic |
| TGTTGGGATCTGATAAGGAT+GGG | 0.548088 | 7.4:+23195765 | None:intergenic |
| TTGTTGGGATCTGATAAGGA+TGG | 0.548205 | 7.4:+23195764 | None:intergenic |
| GCCGGCGATGACGCCGTCGT+AGG | 0.555915 | 7.4:+23195706 | None:intergenic |
| ATCGAGGTGACGGAAATCGA+TGG | 0.556822 | 7.4:+23195919 | None:intergenic |
| CTCCGGCAGAAAATGGAAGC+AGG | 0.556915 | 7.4:-23195678 | MS.gene72422:CDS |
| AGCAATCGAAGATGCCAAGA+TGG | 0.559936 | 7.4:-23195849 | MS.gene72422:CDS |
| GAAGATGACGGAGTTGAAAG+AGG | 0.560293 | 7.4:-23195558 | MS.gene72422:CDS |
| CGAAGCCTTCATCGAGGTGA+CGG | 0.561218 | 7.4:+23195909 | None:intergenic |
| GCAGAGGAAGCAGCTTAGGA+TGG | 0.563867 | 7.4:-23195396 | MS.gene72422:CDS |
| GGCGATAAGGATGAATAAGG+TGG | 0.566855 | 7.4:-23195537 | MS.gene72422:CDS |
| GAAGGAGACTGCGGAGAAGG+TGG | 0.571575 | 7.4:+23195739 | None:intergenic |
| TGGTGGAGCTGCGGTGTCGT+CGG | 0.572732 | 7.4:+23195805 | None:intergenic |
| GAGCTGAAGCGCGACGAGTA+CGG | 0.582697 | 7.4:+23195654 | None:intergenic |
| TCGCTTGGCTGGTGGAGCTG+CGG | 0.593001 | 7.4:+23195796 | None:intergenic |
| CACCTCGATGAAGGCTTCGG+CGG | 0.597988 | 7.4:-23195905 | MS.gene72422:CDS |
| AAGGAGACTGCGGAGAAGGT+GGG | 0.614501 | 7.4:+23195740 | None:intergenic |
| GGGATCTGATAAGGATGGGA+GGG | 0.621088 | 7.4:+23195769 | None:intergenic |
| TTCGGTGCCCCAACCTACGA+CGG | 0.654096 | 7.4:-23195719 | MS.gene72422:CDS |
| TGGGGCACCGAAGGAGACTG+CGG | 0.657319 | 7.4:+23195730 | None:intergenic |
| AAATTGCTAAGTCTAAGCAG+AGG | 0.675462 | 7.4:-23195412 | MS.gene72422:CDS |
| TGGGATCTGATAAGGATGGG+AGG | 0.678962 | 7.4:+23195768 | None:intergenic |
| GGCGTATAAGAAGAAGATGA+CGG | 0.686607 | 7.4:-23195570 | MS.gene72422:CDS |
| GAGAGAGAAGAGCATTAAGG+CGG | 0.691264 | 7.4:-23195591 | MS.gene72422:CDS |
| TGCCGCCGAAGCCTTCATCG+AGG | 0.700268 | 7.4:+23195903 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTCTTAATCATATCATC+AGG | + | chr7.4:23195931-23195950 | None:intergenic | 20.0% |
| !! | GCAATTTTCTTCAAAGTTTT+CGG | + | chr7.4:23195874-23195893 | None:intergenic | 25.0% |
| !!! | AAGGAGAATGTTTTGAAATC+TGG | - | chr7.4:23195823-23195842 | MS.gene72422:CDS | 30.0% |
| AAATTGCTAAGTCTAAGCAG+AGG | - | chr7.4:23195887-23195906 | MS.gene72422:CDS | 35.0% | |
| AGAGGCGATAAGGATGAATA+AGG | - | chr7.4:23195759-23195778 | MS.gene72422:CDS | 40.0% | |
| GATGGAAGAAATCGTATCCA+TGG | - | chr7.4:23195468-23195487 | MS.gene72422:CDS | 40.0% | |
| GGATACGATTTCTTCCATCT+TGG | + | chr7.4:23195467-23195486 | None:intergenic | 40.0% | |
| GGCGTATAAGAAGAAGATGA+CGG | - | chr7.4:23195729-23195748 | MS.gene72422:CDS | 40.0% | |
| TGAATAAGGTGGAGAAGAGA+AGG | - | chr7.4:23195773-23195792 | MS.gene72422:CDS | 40.0% | |
| TGTTGGGATCTGATAAGGAT+GGG | + | chr7.4:23195537-23195556 | None:intergenic | 40.0% | |
| TTGTTGGGATCTGATAAGGA+TGG | + | chr7.4:23195538-23195557 | None:intergenic | 40.0% | |
| AGAGAGAAGAGAGTGAGAAG+AGG | - | chr7.4:23195797-23195816 | MS.gene72422:CDS | 45.0% | |
| AGAGAGTGAGAAGAGGAAGA+AGG | - | chr7.4:23195804-23195823 | MS.gene72422:CDS | 45.0% | |
| AGCAATCGAAGATGCCAAGA+TGG | - | chr7.4:23195450-23195469 | MS.gene72422:CDS | 45.0% | |
| CTTGGCATCTTCGATTGCTT+GGG | + | chr7.4:23195449-23195468 | None:intergenic | 45.0% | |
| GGCGATAAGGATGAATAAGG+TGG | - | chr7.4:23195762-23195781 | MS.gene72422:CDS | 45.0% | |
| TCTTGGCATCTTCGATTGCT+TGG | + | chr7.4:23195450-23195469 | None:intergenic | 45.0% | |
| TTGTTCGCGTTTGCGTTTGT+AGG | + | chr7.4:23195425-23195444 | None:intergenic | 45.0% | |
| !! | AGCGAGAGAGAAGAGCATTA+AGG | - | chr7.4:23195705-23195724 | MS.gene72422:CDS | 45.0% |
| !! | GAAGATGACGGAGTTGAAAG+AGG | - | chr7.4:23195741-23195760 | MS.gene72422:CDS | 45.0% |
| !! | GAGAGAGAAGAGCATTAAGG+CGG | - | chr7.4:23195708-23195727 | MS.gene72422:CDS | 45.0% |
| !! | GGGTTTGTTGGGATCTGATA+AGG | + | chr7.4:23195542-23195561 | None:intergenic | 45.0% |
| AGCTCTTCAGGTGAGCAAGA+AGG | - | chr7.4:23195663-23195682 | MS.gene72422:CDS | 50.0% | |
| CAAGAAGGGAACGACGTTTG+AGG | - | chr7.4:23195678-23195697 | MS.gene72422:CDS | 50.0% | |
| CGGAGTTGAAAGAGGCGATA+AGG | - | chr7.4:23195749-23195768 | MS.gene72422:CDS | 50.0% | |
| CTAAGCAGAGGAAGCAGCTT+AGG | - | chr7.4:23195899-23195918 | MS.gene72422:CDS | 50.0% | |
| GATTTCCGTCACCTCGATGA+AGG | - | chr7.4:23195385-23195404 | MS.gene72422:CDS | 50.0% | |
| GCAAAGTCTCCGGCAGAAAA+TGG | - | chr7.4:23195614-23195633 | MS.gene72422:CDS | 50.0% | |
| GCTCTTCAGGTGAGCAAGAA+GGG | - | chr7.4:23195664-23195683 | MS.gene72422:CDS | 50.0% | |
| GGGATCTGATAAGGATGGGA+GGG | + | chr7.4:23195533-23195552 | None:intergenic | 50.0% | |
| TGGGATCTGATAAGGATGGG+AGG | + | chr7.4:23195534-23195553 | None:intergenic | 50.0% | |
| ! | GACCTGCTTCCATTTTCTGC+CGG | + | chr7.4:23195626-23195645 | None:intergenic | 50.0% |
| ! | TTTTCTGCCGGAGACTTTGC+CGG | + | chr7.4:23195614-23195633 | None:intergenic | 50.0% |
| !! | ATCGAGGTGACGGAAATCGA+TGG | + | chr7.4:23195383-23195402 | None:intergenic | 50.0% |
| AAGGAGACTGCGGAGAAGGT+GGG | + | chr7.4:23195562-23195581 | None:intergenic | 55.0% | |
| ACCTTCTCCGCAGTCTCCTT+CGG | - | chr7.4:23195562-23195581 | MS.gene72422:CDS | 55.0% | |
| CGAAGCCTTCATCGAGGTGA+CGG | + | chr7.4:23195393-23195412 | None:intergenic | 55.0% | |
| CGTCACCTCGATGAAGGCTT+CGG | - | chr7.4:23195391-23195410 | MS.gene72422:CDS | 55.0% | |
| CTCCGGCAGAAAATGGAAGC+AGG | - | chr7.4:23195621-23195640 | MS.gene72422:CDS | 55.0% | |
| GCAGAGGAAGCAGCTTAGGA+TGG | - | chr7.4:23195903-23195922 | MS.gene72422:CDS | 55.0% | |
| ! | GCGGAGAAGGTGGGTTTGTT+GGG | + | chr7.4:23195553-23195572 | None:intergenic | 55.0% |
| ! | GTGACGGAAATCGATGGTGC+AGG | + | chr7.4:23195377-23195396 | None:intergenic | 55.0% |
| ! | TGCGGAGAAGGTGGGTTTGT+TGG | + | chr7.4:23195554-23195573 | None:intergenic | 55.0% |
| ACCGAAGGAGACTGCGGAGA+AGG | + | chr7.4:23195566-23195585 | None:intergenic | 60.0% | |
| CACCTCGATGAAGGCTTCGG+CGG | - | chr7.4:23195394-23195413 | MS.gene72422:CDS | 60.0% | |
| CGATGACGCCGTCGTAGGTT+GGG | + | chr7.4:23195591-23195610 | None:intergenic | 60.0% | |
| GAAGGAGACTGCGGAGAAGG+TGG | + | chr7.4:23195563-23195582 | None:intergenic | 60.0% | |
| GAGCTGAAGCGCGACGAGTA+CGG | + | chr7.4:23195648-23195667 | None:intergenic | 60.0% | |
| GATGACGCCGTCGTAGGTTG+GGG | + | chr7.4:23195590-23195609 | None:intergenic | 60.0% | |
| GTCATCGCCGGCAAAGTCTC+CGG | - | chr7.4:23195604-23195623 | MS.gene72422:CDS | 60.0% | |
| TCGTCGCGCTTCAGCTCTTC+AGG | - | chr7.4:23195651-23195670 | MS.gene72422:CDS | 60.0% | |
| !! | TGCGGTGTCGTCGGTTTCCA+TGG | + | chr7.4:23195488-23195507 | None:intergenic | 60.0% |
| !! | TTCGGTGCCCCAACCTACGA+CGG | - | chr7.4:23195580-23195599 | MS.gene72422:CDS | 60.0% |
| ACCTACGACGGCGTCATCGC+CGG | - | chr7.4:23195592-23195611 | MS.gene72422:CDS | 65.0% | |
| AGGGCAGATCGCTTGGCTGG+TGG | + | chr7.4:23195514-23195533 | None:intergenic | 65.0% | |
| GCGATGACGCCGTCGTAGGT+TGG | + | chr7.4:23195592-23195611 | None:intergenic | 65.0% | |
| GGATGGGAGGGCAGATCGCT+TGG | + | chr7.4:23195521-23195540 | None:intergenic | 65.0% | |
| TCGCTTGGCTGGTGGAGCTG+CGG | + | chr7.4:23195506-23195525 | None:intergenic | 65.0% | |
| TGCCGCCGAAGCCTTCATCG+AGG | + | chr7.4:23195399-23195418 | None:intergenic | 65.0% | |
| !! | GTCGTAGGTTGGGGCACCGA+AGG | + | chr7.4:23195581-23195600 | None:intergenic | 65.0% |
| !! | TGGGGCACCGAAGGAGACTG+CGG | + | chr7.4:23195572-23195591 | None:intergenic | 65.0% |
| !! | TGGTGGAGCTGCGGTGTCGT+CGG | + | chr7.4:23195497-23195516 | None:intergenic | 65.0% |
| GGGAGGGCAGATCGCTTGGC+TGG | + | chr7.4:23195517-23195536 | None:intergenic | 70.0% | |
| GCCGGCGATGACGCCGTCGT+AGG | + | chr7.4:23195596-23195615 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 23195370 | 23195951 | 23195370 | ID=MS.gene72422 |
| chr7.4 | mRNA | 23195370 | 23195951 | 23195370 | ID=MS.gene72422.t1;Parent=MS.gene72422 |
| chr7.4 | exon | 23195370 | 23195951 | 23195370 | ID=MS.gene72422.t1.exon1;Parent=MS.gene72422.t1 |
| chr7.4 | CDS | 23195370 | 23195951 | 23195370 | ID=cds.MS.gene72422.t1;Parent=MS.gene72422.t1 |
| Gene Sequence |
| Protein sequence |
