Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73239.t1 | XP_013454925.1 | 94.2 | 156 | 8 | 1 | 1 | 155 | 1 | 156 | 7.00E-74 | 286.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73239.t1 | A0A072UIR5 | 94.2 | 156 | 8 | 1 | 1 | 155 | 1 | 156 | 5.1e-74 | 286.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059006 | MS.gene73239 | 0.818969 | 1.48E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73239.t1 | MTR_4g019710 | 94.231 | 156 | 8 | 1 | 1 | 155 | 1 | 156 | 9.46e-102 | 288 |
| MS.gene73239.t1 | MTR_7g065150 | 53.237 | 139 | 61 | 3 | 18 | 155 | 21 | 156 | 4.06e-44 | 142 |
| MS.gene73239.t1 | MTR_7g065150 | 52.857 | 140 | 61 | 4 | 18 | 155 | 21 | 157 | 1.60e-42 | 139 |
| MS.gene73239.t1 | MTR_7g065150 | 51.449 | 138 | 63 | 3 | 18 | 154 | 21 | 155 | 1.56e-41 | 136 |
| MS.gene73239.t1 | MTR_4g019690 | 47.799 | 159 | 71 | 5 | 1 | 155 | 1 | 151 | 2.79e-36 | 122 |
| MS.gene73239.t1 | MTR_7g065150 | 50.442 | 113 | 53 | 2 | 18 | 130 | 21 | 130 | 5.58e-31 | 109 |
| MS.gene73239.t1 | MTR_4g034060 | 44.286 | 70 | 38 | 1 | 82 | 150 | 79 | 148 | 3.67e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73239.t1 | AT5G02020 | 50.714 | 140 | 56 | 6 | 17 | 155 | 22 | 149 | 8.66e-30 | 106 |
| MS.gene73239.t1 | AT5G02020 | 45.082 | 122 | 51 | 5 | 17 | 134 | 22 | 131 | 7.26e-19 | 77.8 |
| MS.gene73239.t1 | AT5G59080 | 47.619 | 63 | 27 | 1 | 99 | 155 | 73 | 135 | 4.83e-14 | 65.5 |
| MS.gene73239.t1 | AT5G59080 | 47.619 | 63 | 27 | 1 | 99 | 155 | 73 | 135 | 4.83e-14 | 65.5 |
| MS.gene73239.t1 | AT2G39855 | 35.000 | 140 | 71 | 5 | 18 | 155 | 21 | 142 | 3.09e-13 | 63.5 |
| MS.gene73239.t1 | AT2G39855 | 35.000 | 140 | 71 | 5 | 18 | 155 | 21 | 142 | 3.09e-13 | 63.5 |
| MS.gene73239.t1 | AT3G46880 | 41.176 | 85 | 48 | 1 | 73 | 155 | 32 | 116 | 5.66e-13 | 62.0 |
| MS.gene73239.t1 | AT3G55646 | 34.532 | 139 | 72 | 4 | 18 | 155 | 26 | 146 | 4.35e-12 | 60.5 |
Find 26 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTCTTCTGGAATTTA+TGG | 0.132630 | 4.1:-78501089 | MS.gene73239:CDS |
| TCACCTTGTTCTGAGCTCTT+TGG | 0.224503 | 4.1:-78501137 | MS.gene73239:CDS |
| CACCTTGTTCTGAGCTCTTT+GGG | 0.234708 | 4.1:-78501136 | MS.gene73239:CDS |
| TCATCATCATCTTCTTCTTC+TGG | 0.252399 | 4.1:-78501098 | MS.gene73239:CDS |
| CTTAGTTCATCAATCTATTA+TGG | 0.252777 | 4.1:-78499977 | MS.gene73239:CDS |
| TACAAGAGGGGACTGGTGGC+AGG | 0.416058 | 4.1:-78499599 | MS.gene73239:CDS |
| AGTACCTTGAGTAACAGTTT+TGG | 0.442652 | 4.1:+78500520 | None:intergenic |
| AACATATAGCACAAGAATCC+TGG | 0.479685 | 4.1:-78499643 | MS.gene73239:intron |
| GAGGTTGAGGATATATGTCT+TGG | 0.479701 | 4.1:+78499951 | None:intergenic |
| ATTTGAGAATCTTCTCCTCC+AGG | 0.481127 | 4.1:+78499625 | None:intergenic |
| CATAATAGATTGATGAACTA+AGG | 0.485902 | 4.1:+78499978 | None:intergenic |
| TTCAAAGGAGTAAGAATGGA+AGG | 0.520315 | 4.1:-78501188 | None:intergenic |
| TGGAGGAGAAGATTCTCAAA+TGG | 0.532994 | 4.1:-78499623 | MS.gene73239:CDS |
| ACAAGAGGGGACTGGTGGCA+GGG | 0.569843 | 4.1:-78499598 | MS.gene73239:intron |
| GATAGCATCTTTAAAGACAA+TGG | 0.591645 | 4.1:-78500073 | MS.gene73239:CDS |
| AGTTCATCAATCTATTATGG+TGG | 0.597056 | 4.1:-78499974 | MS.gene73239:CDS |
| AAAACCAAAACTGTTACTCA+AGG | 0.599186 | 4.1:-78500524 | MS.gene73239:intron |
| AAATGGCTACAAGAGGGGAC+TGG | 0.608932 | 4.1:-78499606 | MS.gene73239:CDS |
| TTCTCAAATGGCTACAAGAG+GGG | 0.617613 | 4.1:-78499611 | MS.gene73239:CDS |
| GATTCTCAAATGGCTACAAG+AGG | 0.623491 | 4.1:-78499613 | MS.gene73239:CDS |
| ATTCTCAAATGGCTACAAGA+GGG | 0.629920 | 4.1:-78499612 | MS.gene73239:CDS |
| TGGCTACAAGAGGGGACTGG+TGG | 0.631380 | 4.1:-78499603 | MS.gene73239:CDS |
| GACCCAAAGAGCTCAGAACA+AGG | 0.656538 | 4.1:+78501134 | None:intergenic |
| AGCATCTTTAAAGACAATGG+TGG | 0.682264 | 4.1:-78500070 | MS.gene73239:CDS |
| ATTGATGAACTAAGGTGACA+TGG | 0.710947 | 4.1:+78499986 | None:intergenic |
| ATATAGCACAAGAATCCTGG+AGG | 0.726841 | 4.1:-78499640 | MS.gene73239:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAAAGAATTTTTTTCTAT+AGG | + | chr4.1:78500483-78500502 | None:intergenic | 10.0% |
| !! | AAAAAAAAAATCAAACAAAG+TGG | + | chr4.1:78500897-78500916 | None:intergenic | 15.0% |
| !! | CTTAACCAATTATATTTAAT+GGG | + | chr4.1:78500354-78500373 | None:intergenic | 15.0% |
| !! | TCAAATAAAATTGATGTATA+TGG | - | chr4.1:78500848-78500867 | MS.gene73239:intron | 15.0% |
| !!! | TATTTTTTTATATACTACTC+TGG | - | chr4.1:78500803-78500822 | MS.gene73239:intron | 15.0% |
| !! | AGAAAAAGAAAAAAAAACAG+AGG | + | chr4.1:78500167-78500186 | None:intergenic | 20.0% |
| !! | ATAAAAACAAAACCATGAAA+TGG | + | chr4.1:78499679-78499698 | None:intergenic | 20.0% |
| !! | GCTTAACCAATTATATTTAA+TGG | + | chr4.1:78500355-78500374 | None:intergenic | 20.0% |
| !! | TAAAAACAAAACCATGAAAT+GGG | + | chr4.1:78499678-78499697 | None:intergenic | 20.0% |
| !! | TTTGACCCATTAAATATAAT+TGG | - | chr4.1:78500346-78500365 | MS.gene73239:intron | 20.0% |
| !!! | ATTATGTTGATTCAGATTTT+AGG | - | chr4.1:78500048-78500067 | MS.gene73239:CDS | 20.0% |
| !!! | TGACAAAAATTAGATTTTTG+TGG | - | chr4.1:78501086-78501105 | MS.gene73239:CDS | 20.0% |
| !!! | TTATGTTGATTCAGATTTTA+GGG | - | chr4.1:78500049-78500068 | MS.gene73239:CDS | 20.0% |
| !!! | TTTTTTTTTTACACTCACTT+TGG | + | chr4.1:78500749-78500768 | None:intergenic | 20.0% |
| ! | AAAAACAAAACCATGAAATG+GGG | + | chr4.1:78499677-78499696 | None:intergenic | 25.0% |
| ! | AATCTGAATCAACATAATTC+AGG | + | chr4.1:78500046-78500065 | None:intergenic | 25.0% |
| ! | CATAATAGATTGATGAACTA+AGG | + | chr4.1:78500672-78500691 | None:intergenic | 25.0% |
| ! | CTTAGTTCATCAATCTATTA+TGG | - | chr4.1:78500670-78500689 | MS.gene73239:intron | 25.0% |
| ! | GAAGGAAAAAATAAGCAAAA+GGG | - | chr4.1:78499477-78499496 | MS.gene73239:intron | 25.0% |
| ! | TCAAACAAAGTGGATATATT+TGG | + | chr4.1:78500887-78500906 | None:intergenic | 25.0% |
| ! | TCTGTATTTACATGAGAAAA+AGG | + | chr4.1:78500556-78500575 | None:intergenic | 25.0% |
| ! | TGTAAATATAATACTCCTTC+TGG | + | chr4.1:78500936-78500955 | None:intergenic | 25.0% |
| ! | TGTACATAATGTTCCATATA+TGG | + | chr4.1:78500382-78500401 | None:intergenic | 25.0% |
| !! | GATATGAGTTTTCTCTATAA+GGG | - | chr4.1:78500625-78500644 | MS.gene73239:intron | 25.0% |
| !! | TGATATGAGTTTTCTCTATA+AGG | - | chr4.1:78500624-78500643 | MS.gene73239:intron | 25.0% |
| !!! | AGAAAAAGCATTATTACCTT+AGG | + | chr4.1:78499606-78499625 | None:intergenic | 25.0% |
| !!! | AGTAACAGCGTATTTAATTT+TGG | - | chr4.1:78500246-78500265 | MS.gene73239:intron | 25.0% |
| !!! | ATTGTTTTACCTAAATCTCT+AGG | - | chr4.1:78500434-78500453 | MS.gene73239:intron | 25.0% |
| !!! | GAAGGAGTATTATATTTACA+TGG | - | chr4.1:78500936-78500955 | MS.gene73239:intron | 25.0% |
| AAAACCAAAACTGTTACTCA+AGG | - | chr4.1:78500123-78500142 | MS.gene73239:intron | 30.0% | |
| AAGTTCATTGAACATGGATA+TGG | - | chr4.1:78499998-78500017 | MS.gene73239:CDS | 30.0% | |
| AGGTGATTGAGTAGAAAAAA+TGG | + | chr4.1:78499586-78499605 | None:intergenic | 30.0% | |
| AGTTCATCAATCTATTATGG+TGG | - | chr4.1:78500673-78500692 | MS.gene73239:intron | 30.0% | |
| ATAACCAGATGCATGAAATA+AGG | + | chr4.1:78499924-78499943 | None:intergenic | 30.0% | |
| GCATGAAATAAGGTTGAAAT+AGG | + | chr4.1:78499914-78499933 | None:intergenic | 30.0% | |
| GGAAGGAAAAAATAAGCAAA+AGG | - | chr4.1:78499476-78499495 | MS.gene73239:intron | 30.0% | |
| TGTAACAAGTTCATTGAACA+TGG | - | chr4.1:78499992-78500011 | MS.gene73239:CDS | 30.0% | |
| ! | ACTTGTTACATGATACAACA+TGG | + | chr4.1:78499982-78500001 | None:intergenic | 30.0% |
| ! | GATAGCATCTTTAAAGACAA+TGG | - | chr4.1:78500574-78500593 | MS.gene73239:CDS | 30.0% |
| ! | TGGTTAAGCTTTTCCATATA+TGG | - | chr4.1:78500366-78500385 | MS.gene73239:intron | 30.0% |
| ! | TGTTTATATTTGAGACCAGA+AGG | - | chr4.1:78500918-78500937 | MS.gene73239:intron | 30.0% |
| ! | TTTTATTCAACCCCATTTCA+TGG | - | chr4.1:78499664-78499683 | MS.gene73239:intron | 30.0% |
| ! | TTTTTCTACTCAATCACCTA+AGG | - | chr4.1:78499587-78499606 | MS.gene73239:intron | 30.0% |
| !! | TCTTCTTCTTCTGGAATTTA+TGG | - | chr4.1:78499558-78499577 | MS.gene73239:intron | 30.0% |
| AACATATAGCACAAGAATCC+TGG | - | chr4.1:78501004-78501023 | MS.gene73239:intron | 35.0% | |
| ATTCTCAAATGGCTACAAGA+GGG | - | chr4.1:78501035-78501054 | MS.gene73239:intron | 35.0% | |
| ATTGATGAACTAAGGTGACA+TGG | + | chr4.1:78500664-78500683 | None:intergenic | 35.0% | |
| TACATTGCACCTAGAGATTT+AGG | + | chr4.1:78500446-78500465 | None:intergenic | 35.0% | |
| TCAATAATAGAGACTTCCTG+TGG | + | chr4.1:78501176-78501195 | None:intergenic | 35.0% | |
| TCATCATCATCTTCTTCTTC+TGG | - | chr4.1:78499549-78499568 | MS.gene73239:intron | 35.0% | |
| TTGCTTTCAGACATGAATGT+TGG | + | chr4.1:78499779-78499798 | None:intergenic | 35.0% | |
| ! | ACATGTTAGTCTCGTGTAAA+CGG | - | chr4.1:78499874-78499893 | MS.gene73239:intron | 35.0% |
| ! | AGCATCTTTAAAGACAATGG+TGG | - | chr4.1:78500577-78500596 | MS.gene73239:CDS | 35.0% |
| ! | AGTACCTTGAGTAACAGTTT+TGG | + | chr4.1:78500130-78500149 | None:intergenic | 35.0% |
| ! | TCAACCTTATTTCATGCATC+TGG | - | chr4.1:78499917-78499936 | MS.gene73239:intron | 35.0% |
| !! | CTTGTTATCTTTTGATCCAC+AGG | - | chr4.1:78501157-78501176 | MS.gene73239:CDS | 35.0% |
| !!! | TTTTGAGTACTTTGAGGTTG+AGG | + | chr4.1:78500712-78500731 | None:intergenic | 35.0% |
| ATATAGCACAAGAATCCTGG+AGG | - | chr4.1:78501007-78501026 | MS.gene73239:intron | 40.0% | |
| ATTTGAGAATCTTCTCCTCC+AGG | + | chr4.1:78501025-78501044 | None:intergenic | 40.0% | |
| GAGGTTGAGGATATATGTCT+TGG | + | chr4.1:78500699-78500718 | None:intergenic | 40.0% | |
| GATTCTCAAATGGCTACAAG+AGG | - | chr4.1:78501034-78501053 | MS.gene73239:intron | 40.0% | |
| TGGAGGAGAAGATTCTCAAA+TGG | - | chr4.1:78501024-78501043 | MS.gene73239:intron | 40.0% | |
| TTCTCAAATGGCTACAAGAG+GGG | - | chr4.1:78501036-78501055 | MS.gene73239:intron | 40.0% | |
| !! | GCAGCATTTTGAGTACTTTG+AGG | + | chr4.1:78500718-78500737 | None:intergenic | 40.0% |
| ! | CACCTTGTTCTGAGCTCTTT+GGG | - | chr4.1:78499511-78499530 | MS.gene73239:intron | 45.0% |
| ! | TCACCTTGTTCTGAGCTCTT+TGG | - | chr4.1:78499510-78499529 | MS.gene73239:intron | 45.0% |
| !! | AAAAGTTATTAATTATTATT+TGG | + | chr4.1:78500281-78500300 | None:intergenic | 5.0% |
| !! | CAATATATTAAAATATAATA+TGG | - | chr4.1:78500214-78500233 | MS.gene73239:intron | 5.0% |
| AAATGGCTACAAGAGGGGAC+TGG | - | chr4.1:78501041-78501060 | MS.gene73239:intron | 50.0% | |
| GACCCAAAGAGCTCAGAACA+AGG | + | chr4.1:78499516-78499535 | None:intergenic | 50.0% | |
| TGGCTACAAGAGGGGACTGG+TGG | - | chr4.1:78501044-78501063 | MS.gene73239:intron | 60.0% | |
| ! | ACAAGAGGGGACTGGTGGCA+GGG | - | chr4.1:78501049-78501068 | MS.gene73239:intron | 60.0% |
| ! | TACAAGAGGGGACTGGTGGC+AGG | - | chr4.1:78501048-78501067 | MS.gene73239:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 78499474 | 78501195 | 78499474 | ID=MS.gene73239 |
| chr4.1 | mRNA | 78499474 | 78501195 | 78499474 | ID=MS.gene73239.t1;Parent=MS.gene73239 |
| chr4.1 | exon | 78501061 | 78501195 | 78501061 | ID=MS.gene73239.t1.exon1;Parent=MS.gene73239.t1 |
| chr4.1 | CDS | 78501061 | 78501195 | 78501061 | ID=cds.MS.gene73239.t1;Parent=MS.gene73239.t1 |
| chr4.1 | exon | 78500525 | 78500606 | 78500525 | ID=MS.gene73239.t1.exon2;Parent=MS.gene73239.t1 |
| chr4.1 | CDS | 78500525 | 78500606 | 78500525 | ID=cds.MS.gene73239.t1;Parent=MS.gene73239.t1 |
| chr4.1 | exon | 78499919 | 78500094 | 78499919 | ID=MS.gene73239.t1.exon3;Parent=MS.gene73239.t1 |
| chr4.1 | CDS | 78499919 | 78500094 | 78499919 | ID=cds.MS.gene73239.t1;Parent=MS.gene73239.t1 |
| chr4.1 | exon | 78499599 | 78499656 | 78499599 | ID=MS.gene73239.t1.exon4;Parent=MS.gene73239.t1 |
| chr4.1 | CDS | 78499599 | 78499656 | 78499599 | ID=cds.MS.gene73239.t1;Parent=MS.gene73239.t1 |
| chr4.1 | exon | 78499474 | 78499490 | 78499474 | ID=MS.gene73239.t1.exon5;Parent=MS.gene73239.t1 |
| chr4.1 | CDS | 78499474 | 78499490 | 78499474 | ID=cds.MS.gene73239.t1;Parent=MS.gene73239.t1 |
| Gene Sequence |
| Protein sequence |
