Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73356.t1 | RHN47394.1 | 90 | 130 | 13 | 0 | 8 | 137 | 101 | 230 | 2.80E-58 | 234.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73356.t1 | Q9AVK0 | 41.4 | 133 | 72 | 1 | 5 | 137 | 241 | 367 | 9.5e-23 | 107.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73356.t1 | G7KYA2 | 90.0 | 130 | 13 | 0 | 8 | 137 | 236 | 365 | 2.0e-58 | 234.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049915 | MS.gene73356 | 0.803555 | 3.30E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene73356 | 0.806425 | 8.28E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73356.t1 | MTR_7g084620 | 87.970 | 133 | 16 | 0 | 5 | 137 | 233 | 365 | 9.37e-81 | 242 |
| MS.gene73356.t1 | MTR_7g084330 | 87.121 | 132 | 17 | 0 | 6 | 137 | 202 | 333 | 3.98e-78 | 234 |
| MS.gene73356.t1 | MTR_7g084350 | 78.030 | 132 | 29 | 0 | 6 | 137 | 234 | 365 | 8.85e-70 | 214 |
| MS.gene73356.t1 | MTR_3g467560 | 79.389 | 131 | 24 | 1 | 5 | 135 | 260 | 387 | 2.65e-68 | 211 |
| MS.gene73356.t1 | MTR_7g084340 | 65.891 | 129 | 44 | 0 | 7 | 135 | 1 | 129 | 1.74e-55 | 170 |
| MS.gene73356.t1 | MTR_6g015620 | 46.032 | 126 | 67 | 1 | 12 | 136 | 12 | 137 | 2.40e-33 | 114 |
| MS.gene73356.t1 | MTR_3g117060 | 46.512 | 129 | 68 | 1 | 8 | 135 | 239 | 367 | 2.99e-32 | 117 |
| MS.gene73356.t1 | MTR_4g018820 | 37.405 | 131 | 75 | 2 | 5 | 135 | 234 | 357 | 3.43e-25 | 98.2 |
| MS.gene73356.t1 | MTR_1g022465 | 40.458 | 131 | 74 | 1 | 5 | 135 | 250 | 376 | 1.15e-24 | 97.1 |
| MS.gene73356.t1 | MTR_5g020940 | 37.778 | 135 | 80 | 2 | 5 | 135 | 245 | 379 | 1.79e-24 | 96.7 |
| MS.gene73356.t1 | MTR_8g077040 | 36.691 | 139 | 80 | 4 | 5 | 137 | 234 | 370 | 1.85e-24 | 96.7 |
| MS.gene73356.t1 | MTR_1g022455 | 40.458 | 131 | 75 | 1 | 5 | 135 | 250 | 377 | 1.36e-23 | 94.4 |
| MS.gene73356.t1 | MTR_3g467270 | 33.813 | 139 | 84 | 2 | 5 | 137 | 233 | 369 | 3.76e-23 | 92.8 |
| MS.gene73356.t1 | MTR_8g076900 | 35.115 | 131 | 81 | 1 | 5 | 135 | 234 | 360 | 4.33e-22 | 90.1 |
| MS.gene73356.t1 | MTR_8g020690 | 34.351 | 131 | 79 | 2 | 5 | 135 | 235 | 358 | 4.71e-20 | 84.3 |
| MS.gene73356.t1 | MTR_6g056070 | 33.083 | 133 | 79 | 3 | 5 | 137 | 235 | 357 | 5.35e-20 | 84.3 |
| MS.gene73356.t1 | MTR_8g020700 | 34.351 | 131 | 76 | 2 | 5 | 135 | 235 | 355 | 7.79e-19 | 81.3 |
| MS.gene73356.t1 | MTR_5g093900 | 32.540 | 126 | 78 | 2 | 11 | 134 | 269 | 389 | 2.12e-15 | 71.6 |
| MS.gene73356.t1 | MTR_3g052700 | 31.746 | 126 | 79 | 2 | 11 | 134 | 267 | 387 | 1.67e-14 | 69.3 |
| MS.gene73356.t1 | MTR_3g054330 | 33.010 | 103 | 64 | 1 | 11 | 113 | 260 | 357 | 4.82e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73356.t1 | AT1G19640 | 36.029 | 136 | 82 | 2 | 5 | 135 | 252 | 387 | 2.82e-25 | 99.0 |
| MS.gene73356.t1 | AT3G11480 | 37.405 | 131 | 75 | 1 | 5 | 135 | 255 | 378 | 2.55e-20 | 85.5 |
| MS.gene73356.t1 | AT5G04370 | 38.931 | 131 | 75 | 3 | 5 | 135 | 264 | 389 | 4.86e-20 | 84.7 |
| MS.gene73356.t1 | AT5G04370 | 38.931 | 131 | 75 | 3 | 5 | 135 | 269 | 394 | 5.98e-20 | 84.7 |
| MS.gene73356.t1 | AT5G66430 | 40.000 | 115 | 65 | 2 | 5 | 117 | 223 | 335 | 1.16e-19 | 83.6 |
| MS.gene73356.t1 | AT5G04370 | 38.931 | 131 | 75 | 3 | 5 | 135 | 283 | 408 | 1.38e-18 | 80.9 |
| MS.gene73356.t1 | AT5G38020 | 37.069 | 116 | 60 | 3 | 12 | 117 | 197 | 309 | 5.12e-16 | 73.2 |
| MS.gene73356.t1 | AT5G38020 | 37.069 | 116 | 60 | 3 | 12 | 117 | 236 | 348 | 6.72e-16 | 73.2 |
| MS.gene73356.t1 | AT5G04380 | 34.513 | 113 | 72 | 1 | 5 | 117 | 189 | 299 | 1.43e-14 | 68.9 |
| MS.gene73356.t1 | AT5G04380 | 34.513 | 113 | 72 | 1 | 5 | 117 | 260 | 370 | 1.98e-14 | 68.9 |
| MS.gene73356.t1 | AT4G36470 | 28.244 | 131 | 87 | 1 | 5 | 135 | 246 | 369 | 3.87e-14 | 68.2 |
| MS.gene73356.t1 | AT3G21950 | 37.069 | 116 | 60 | 3 | 12 | 117 | 236 | 348 | 6.72e-13 | 64.7 |
| MS.gene73356.t1 | AT5G38100 | 30.909 | 110 | 62 | 2 | 9 | 117 | 219 | 315 | 1.28e-11 | 60.8 |
| MS.gene73356.t1 | AT2G14060 | 29.730 | 111 | 77 | 1 | 14 | 123 | 246 | 356 | 1.32e-11 | 60.8 |
| MS.gene73356.t1 | AT5G38100 | 31.193 | 109 | 61 | 2 | 9 | 116 | 242 | 337 | 1.46e-11 | 60.8 |
| MS.gene73356.t1 | AT3G21950 | 36.607 | 112 | 58 | 3 | 16 | 117 | 272 | 380 | 1.49e-11 | 60.8 |
| MS.gene73356.t1 | AT5G55250 | 32.000 | 100 | 63 | 1 | 11 | 110 | 226 | 320 | 2.13e-11 | 60.5 |
| MS.gene73356.t1 | AT5G55250 | 32.000 | 100 | 63 | 1 | 11 | 110 | 264 | 358 | 2.63e-11 | 60.1 |
Find 20 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAGTTGAAGAAGCAAAAT+TGG | 0.215168 | 7.2:+23022438 | MS.gene73356:CDS |
| TTACCATATTTAAGGATTCT+TGG | 0.276503 | 7.2:-23022788 | None:intergenic |
| TTGTAGATAGCAAAATGAAA+GGG | 0.439719 | 7.2:+23022605 | MS.gene73356:CDS |
| TGCTTCACTTCCTCTATAGT+AGG | 0.445450 | 7.2:-23022487 | None:intergenic |
| CCAAACCCGGCTATTAAAAG+AGG | 0.479976 | 7.2:-23022664 | None:intergenic |
| ATAAACAGCTCTGTGATACT+TGG | 0.484175 | 7.2:-23022638 | None:intergenic |
| TCATGGATGAATTGTTCTCA+AGG | 0.497534 | 7.2:+23022695 | MS.gene73356:CDS |
| GTTGTAGATAGCAAAATGAA+AGG | 0.531131 | 7.2:+23022604 | MS.gene73356:CDS |
| CATGCAGGTATTTCTCCTCA+TGG | 0.532438 | 7.2:+23021955 | None:intergenic |
| AATCAACATTGTCTTTCTCA+AGG | 0.538157 | 7.2:-23022579 | None:intergenic |
| TATGTATCTTCCTACTATAG+AGG | 0.551574 | 7.2:+23022477 | MS.gene73356:CDS |
| ACTCATTGAGATAGAGACAT+TGG | 0.576407 | 7.2:+23022738 | MS.gene73356:CDS |
| TCTATAGTAGGAAGATACAT+AGG | 0.592931 | 7.2:-23022475 | None:intergenic |
| AAGCAAGTGATCGAGACAGA+AGG | 0.596953 | 7.2:+23022505 | MS.gene73356:CDS |
| CATGGTGGTACTCAATGAGA+TGG | 0.597277 | 7.2:+23021973 | MS.gene73356:CDS |
| TGTAGATAGCAAAATGAAAG+GGG | 0.604335 | 7.2:+23022606 | MS.gene73356:CDS |
| CGGGTTTGGAGAAAACATCA+TGG | 0.634728 | 7.2:+23022678 | MS.gene73356:CDS |
| GCAGGTATTTCTCCTCATGG+TGG | 0.667435 | 7.2:+23021958 | MS.gene73356:CDS |
| ACTCAATGAGATGGTCCAAG+AGG | 0.686877 | 7.2:+23021982 | MS.gene73356:CDS |
| TCTCATTGAGTACCACCATG+AGG | 0.763377 | 7.2:-23021970 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATTAAAACTTTTAAAA+TGG | + | chr7.2:23022543-23022562 | MS.gene73356:CDS | 10.0% |
| !!! | ACAATTAAAACTTTTAAAAT+GGG | + | chr7.2:23022544-23022563 | MS.gene73356:CDS | 10.0% |
| !! | ATACTACTAGTATATTATAA+TGG | + | chr7.2:23022193-23022212 | MS.gene73356:intron | 15.0% |
| !! | TTTATAATGATTAATGACTA+TGG | + | chr7.2:23022265-23022284 | MS.gene73356:intron | 15.0% |
| !!! | AAAAGTTTTAATTGTTTGAA+GGG | - | chr7.2:23022539-23022558 | None:intergenic | 15.0% |
| !!! | ACAAAACATTATTTTTATCA+AGG | - | chr7.2:23022378-23022397 | None:intergenic | 15.0% |
| !!! | CTTTTCTATAAAATTTTGTA+GGG | + | chr7.2:23022415-23022434 | MS.gene73356:intron | 15.0% |
| !!! | TAAAAGTTTTAATTGTTTGA+AGG | - | chr7.2:23022540-23022559 | None:intergenic | 15.0% |
| !! | CATTAATCATTATAAAAAGC+AGG | - | chr7.2:23022261-23022280 | None:intergenic | 20.0% |
| !!! | ATCAATATTTCTAACGATTT+AGG | + | chr7.2:23022104-23022123 | MS.gene73356:intron | 20.0% |
| !!! | CCTACAAAATTTTATAGAAA+AGG | - | chr7.2:23022417-23022436 | None:intergenic | 20.0% |
| !!! | CCTTTTCTATAAAATTTTGT+AGG | + | chr7.2:23022414-23022433 | MS.gene73356:intron | 20.0% |
| !!! | TAAAACTTTTAAAATGGGTT+GGG | + | chr7.2:23022549-23022568 | MS.gene73356:CDS | 20.0% |
| !!! | TAGTTATTACTTTGAAAGTT+AGG | - | chr7.2:23022237-23022256 | None:intergenic | 20.0% |
| !!! | TTAAAACTTTTAAAATGGGT+TGG | + | chr7.2:23022548-23022567 | MS.gene73356:CDS | 20.0% |
| ! | AACCATTATTTATTACCTCT+TGG | - | chr7.2:23022000-23022019 | None:intergenic | 25.0% |
| ! | AAGAGGTAATAAATAATGGT+TGG | + | chr7.2:23021999-23022018 | MS.gene73356:intron | 25.0% |
| ! | AATCAAGACTTAAAATGAGT+TGG | + | chr7.2:23022344-23022363 | MS.gene73356:intron | 25.0% |
| ! | ATAATGATTAATGACTATGG+AGG | + | chr7.2:23022268-23022287 | MS.gene73356:intron | 25.0% |
| ! | ATCAAGACTTAAAATGAGTT+GGG | + | chr7.2:23022345-23022364 | MS.gene73356:intron | 25.0% |
| ! | TTGTAGATAGCAAAATGAAA+GGG | + | chr7.2:23022605-23022624 | MS.gene73356:CDS | 25.0% |
| ! | TTTAAGTCTTGATTGTTTGA+AGG | - | chr7.2:23022338-23022357 | None:intergenic | 25.0% |
| ! | TTTAGTTGAAGAAGCAAAAT+TGG | + | chr7.2:23022438-23022457 | MS.gene73356:CDS | 25.0% |
| !! | GTAATAAATAATGGTTGGTA+GGG | + | chr7.2:23022004-23022023 | MS.gene73356:intron | 25.0% |
| !! | TATTACTTTGAAAGTTAGGT+AGG | - | chr7.2:23022233-23022252 | None:intergenic | 25.0% |
| AATCAACATTGTCTTTCTCA+AGG | - | chr7.2:23022582-23022601 | None:intergenic | 30.0% | |
| CATAGTTTCTATCCTGTAAA+AGG | - | chr7.2:23022135-23022154 | None:intergenic | 30.0% | |
| GGTAATAAATAATGGTTGGT+AGG | + | chr7.2:23022003-23022022 | MS.gene73356:intron | 30.0% | |
| GTCCAAGAGGTAATAAATAA+TGG | + | chr7.2:23021995-23022014 | MS.gene73356:intron | 30.0% | |
| GTTGTAGATAGCAAAATGAA+AGG | + | chr7.2:23022604-23022623 | MS.gene73356:CDS | 30.0% | |
| TATGTATCTTCCTACTATAG+AGG | + | chr7.2:23022477-23022496 | MS.gene73356:CDS | 30.0% | |
| TCTATAGTAGGAAGATACAT+AGG | - | chr7.2:23022478-23022497 | None:intergenic | 30.0% | |
| TGTAGATAGCAAAATGAAAG+GGG | + | chr7.2:23022606-23022625 | MS.gene73356:CDS | 30.0% | |
| ! | TATGAACCTCTTTTAATAGC+CGG | + | chr7.2:23022658-23022677 | MS.gene73356:CDS | 30.0% |
| ACTCATTGAGATAGAGACAT+TGG | + | chr7.2:23022738-23022757 | MS.gene73356:CDS | 35.0% | |
| ATAAACAGCTCTGTGATACT+TGG | - | chr7.2:23022641-23022660 | None:intergenic | 35.0% | |
| TCATGGATGAATTGTTCTCA+AGG | + | chr7.2:23022695-23022714 | MS.gene73356:CDS | 35.0% | |
| TTGATTGTTTGAAGGCCAAA+GGG | - | chr7.2:23022330-23022349 | None:intergenic | 35.0% | |
| ! | ATGAACCTCTTTTAATAGCC+GGG | + | chr7.2:23022659-23022678 | MS.gene73356:CDS | 35.0% |
| ! | GGAATTAAGACTACTGATTG+AGG | + | chr7.2:23022289-23022308 | MS.gene73356:intron | 35.0% |
| CAGAAGGATCTTTAACCCTT+TGG | + | chr7.2:23022312-23022331 | MS.gene73356:intron | 40.0% | |
| CTTGATTGTTTGAAGGCCAA+AGG | - | chr7.2:23022331-23022350 | None:intergenic | 40.0% | |
| TGCTTCACTTCCTCTATAGT+AGG | - | chr7.2:23022490-23022509 | None:intergenic | 40.0% | |
| ! | CATGATGTTTTCTCCAAACC+CGG | - | chr7.2:23022680-23022699 | None:intergenic | 40.0% |
| !! | ATTTAGGCACTGCCTTTTAC+AGG | + | chr7.2:23022120-23022139 | MS.gene73356:intron | 40.0% |
| AAGCAAGTGATCGAGACAGA+AGG | + | chr7.2:23022505-23022524 | MS.gene73356:CDS | 45.0% | |
| ACTCAATGAGATGGTCCAAG+AGG | + | chr7.2:23021982-23022001 | MS.gene73356:CDS | 45.0% | |
| CATGGTGGTACTCAATGAGA+TGG | + | chr7.2:23021973-23021992 | MS.gene73356:CDS | 45.0% | |
| CCAAACCCGGCTATTAAAAG+AGG | - | chr7.2:23022667-23022686 | None:intergenic | 45.0% | |
| CGGGTTTGGAGAAAACATCA+TGG | + | chr7.2:23022678-23022697 | MS.gene73356:CDS | 45.0% | |
| TCTCATTGAGTACCACCATG+AGG | - | chr7.2:23021973-23021992 | None:intergenic | 45.0% | |
| ! | AGACTACTGATTGAGGCAGA+AGG | + | chr7.2:23022296-23022315 | MS.gene73356:intron | 45.0% |
| ! | CCTCTTTTAATAGCCGGGTT+TGG | + | chr7.2:23022664-23022683 | MS.gene73356:CDS | 45.0% |
| GCAGGTATTTCTCCTCATGG+TGG | + | chr7.2:23021958-23021977 | MS.gene73356:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 23021956 | 23022801 | 23021956 | ID=MS.gene73356 |
| chr7.2 | mRNA | 23021956 | 23022801 | 23021956 | ID=MS.gene73356.t1;Parent=MS.gene73356 |
| chr7.2 | exon | 23021956 | 23022003 | 23021956 | ID=MS.gene73356.t1.exon1;Parent=MS.gene73356.t1 |
| chr7.2 | CDS | 23021956 | 23022003 | 23021956 | ID=cds.MS.gene73356.t1;Parent=MS.gene73356.t1 |
| chr7.2 | exon | 23022436 | 23022801 | 23022436 | ID=MS.gene73356.t1.exon2;Parent=MS.gene73356.t1 |
| chr7.2 | CDS | 23022436 | 23022801 | 23022436 | ID=cds.MS.gene73356.t1;Parent=MS.gene73356.t1 |
| Gene Sequence |
| Protein sequence |
