Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74083.t1 | XP_013447883.1 | 93.1 | 175 | 12 | 0 | 1 | 175 | 1 | 175 | 1.70E-87 | 332 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74083.t1 | O31633 | 28.7 | 171 | 109 | 4 | 1 | 170 | 21 | 179 | 8.2e-11 | 68.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74083.t1 | I3T613 | 93.1 | 175 | 12 | 0 | 1 | 175 | 1 | 175 | 1.2e-87 | 332.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene74083 | 0.820739 | 5.85E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74083.t1 | MTR_7g024765 | 93.143 | 175 | 12 | 0 | 1 | 175 | 1 | 175 | 1.43e-119 | 335 |
| MS.gene74083.t1 | MTR_7g024760 | 80.347 | 173 | 34 | 0 | 1 | 173 | 1 | 173 | 1.57e-100 | 287 |
| MS.gene74083.t1 | MTR_7g024780 | 68.786 | 173 | 53 | 1 | 1 | 172 | 1 | 173 | 2.50e-84 | 246 |
| MS.gene74083.t1 | MTR_7g114410 | 60.119 | 168 | 65 | 2 | 7 | 172 | 7 | 174 | 1.45e-64 | 196 |
| MS.gene74083.t1 | MTR_7g114410 | 59.333 | 150 | 59 | 2 | 7 | 154 | 7 | 156 | 9.99e-56 | 173 |
| MS.gene74083.t1 | MTR_3g463610 | 46.429 | 168 | 85 | 2 | 7 | 169 | 6 | 173 | 2.54e-47 | 154 |
| MS.gene74083.t1 | MTR_1g103110 | 42.771 | 166 | 92 | 3 | 7 | 169 | 22 | 187 | 1.33e-38 | 130 |
| MS.gene74083.t1 | MTR_5g006470 | 41.566 | 166 | 94 | 2 | 7 | 169 | 20 | 185 | 5.44e-36 | 124 |
| MS.gene74083.t1 | MTR_5g006510 | 42.262 | 168 | 91 | 4 | 7 | 169 | 22 | 188 | 1.13e-35 | 123 |
| MS.gene74083.t1 | MTR_5g006450 | 39.881 | 168 | 96 | 3 | 7 | 169 | 20 | 187 | 3.90e-35 | 122 |
| MS.gene74083.t1 | MTR_1g103150 | 40.964 | 166 | 95 | 3 | 7 | 169 | 22 | 187 | 4.89e-35 | 121 |
| MS.gene74083.t1 | MTR_5g006460 | 41.520 | 171 | 91 | 6 | 7 | 169 | 21 | 190 | 1.09e-34 | 120 |
| MS.gene74083.t1 | MTR_5g006520 | 38.690 | 168 | 98 | 3 | 7 | 169 | 1738 | 1905 | 5.52e-28 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74083.t1 | AT2G32020 | 41.040 | 173 | 98 | 3 | 1 | 171 | 12 | 182 | 1.10e-44 | 146 |
| MS.gene74083.t1 | AT2G32030 | 40.964 | 166 | 94 | 3 | 8 | 171 | 24 | 187 | 3.35e-42 | 140 |
| MS.gene74083.t1 | AT3G22560 | 42.771 | 166 | 91 | 3 | 8 | 169 | 7 | 172 | 3.35e-37 | 126 |
Find 34 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCTATTGGGTTCAATTAT+TGG | 0.148532 | 7.4:+76626054 | MS.gene74083:CDS |
| TGCTATTGGGTTCAATTATT+GGG | 0.160306 | 7.4:+76626055 | MS.gene74083:CDS |
| TGCAAAGTTTAAAGCAGATT+TGG | 0.219727 | 7.4:+76626028 | MS.gene74083:CDS |
| TCCTGACTTGCAAAGGTTGC+AGG | 0.286836 | 7.4:+76626136 | MS.gene74083:CDS |
| GCCTGCAACCTTTGCAAGTC+AGG | 0.340339 | 7.4:-76626137 | None:intergenic |
| TGTCTTGATGATCACTCTAT+TGG | 0.344658 | 7.4:+76625978 | MS.gene74083:CDS |
| GCAAAGTTTAAAGCAGATTT+GGG | 0.349624 | 7.4:+76626029 | MS.gene74083:CDS |
| TTAAACTTTGCATAAACAAT+TGG | 0.394119 | 7.4:-76626017 | None:intergenic |
| CATCAAGACATATTGATTGT+TGG | 0.420749 | 7.4:-76625965 | None:intergenic |
| TCGGCATCACTTTCTTTGAA+TGG | 0.427869 | 7.4:-76625828 | None:intergenic |
| TTTGCAAGTCAGGAAACTCT+TGG | 0.450184 | 7.4:-76626127 | None:intergenic |
| AGACATATTGATTGTTGGAA+TGG | 0.451178 | 7.4:-76625960 | None:intergenic |
| TTCTGAACAATCCTTCCCTA+TGG | 0.465225 | 7.4:-76626214 | None:intergenic |
| CATCACTTTCTTTGAATGGC+CGG | 0.466310 | 7.4:-76625824 | None:intergenic |
| GATTGGAGTTGTGTAAATCA+AGG | 0.488742 | 7.4:+76625898 | MS.gene74083:CDS |
| GGGTTCAATTATTGGGGCCA+AGG | 0.491840 | 7.4:+76626062 | MS.gene74083:CDS |
| GCTATTGGGTTCAATTATTG+GGG | 0.503790 | 7.4:+76626056 | MS.gene74083:CDS |
| AGACAACATGGTTTCACAAA+GGG | 0.505960 | 7.4:+76626172 | MS.gene74083:CDS |
| TCATCACAAAGGGAAAATTG+AGG | 0.519090 | 7.4:+76626244 | MS.gene74083:CDS |
| AGTCATTCAAAATACTCGAT+TGG | 0.520421 | 7.4:+76625881 | MS.gene74083:CDS |
| CATGGTTTCACAAAGGGTGT+TGG | 0.523587 | 7.4:+76626178 | MS.gene74083:CDS |
| AAGAGTTTCCTGACTTGCAA+AGG | 0.529380 | 7.4:+76626129 | MS.gene74083:CDS |
| TGCCGACGATGTGTTGTTGT+GGG | 0.540042 | 7.4:+76625845 | MS.gene74083:CDS |
| AAAGTTCGATTCCATAGGGA+AGG | 0.559980 | 7.4:+76626203 | MS.gene74083:CDS |
| TAGGGAAGGATTGTTCAGAA+AGG | 0.565276 | 7.4:+76626217 | MS.gene74083:CDS |
| ATGCCGACGATGTGTTGTTG+TGG | 0.566383 | 7.4:+76625844 | MS.gene74083:CDS |
| TCATGAGGAATTTCATCAGT+AGG | 0.566992 | 7.4:-76626290 | None:intergenic |
| ATTGGAGTTGTGTAAATCAA+GGG | 0.577493 | 7.4:+76625899 | MS.gene74083:CDS |
| ATGAGTCAAGGATCACTCTC+CGG | 0.583353 | 7.4:+76625805 | MS.gene74083:CDS |
| ACTAATCAAACAACATCATG+AGG | 0.616668 | 7.4:-76626305 | None:intergenic |
| TAATATTCTTGTAGACAACA+TGG | 0.625936 | 7.4:+76626160 | MS.gene74083:CDS |
| TGCCCACAACAACACATCGT+CGG | 0.630490 | 7.4:-76625847 | None:intergenic |
| TAGACAACATGGTTTCACAA+AGG | 0.637639 | 7.4:+76626171 | MS.gene74083:CDS |
| TGGGAATAGATCATGAGTCA+AGG | 0.642231 | 7.4:+76625793 | MS.gene74083:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAACTTTGCATAAACAAT+TGG | - | chr7.4:76626020-76626039 | None:intergenic | 20.0% |
| ! | AGGCAAGAAACTAAAAATAT+AGG | - | chr7.4:76626273-76626292 | None:intergenic | 25.0% |
| ! | TAATATTCTTGTAGACAACA+TGG | + | chr7.4:76626160-76626179 | MS.gene74083:CDS | 25.0% |
| AGTCATTCAAAATACTCGAT+TGG | + | chr7.4:76625881-76625900 | MS.gene74083:CDS | 30.0% | |
| ATTGGAGTTGTGTAAATCAA+GGG | + | chr7.4:76625899-76625918 | MS.gene74083:CDS | 30.0% | |
| TGCTATTGGGTTCAATTATT+GGG | + | chr7.4:76626055-76626074 | MS.gene74083:CDS | 30.0% | |
| ! | AGAAAGGTTTTTCATCACAA+AGG | + | chr7.4:76626233-76626252 | MS.gene74083:CDS | 30.0% |
| ! | AGACATATTGATTGTTGGAA+TGG | - | chr7.4:76625963-76625982 | None:intergenic | 30.0% |
| ! | CATCAAGACATATTGATTGT+TGG | - | chr7.4:76625968-76625987 | None:intergenic | 30.0% |
| ! | GAAAGGTTTTTCATCACAAA+GGG | + | chr7.4:76626234-76626253 | MS.gene74083:CDS | 30.0% |
| ! | GCAAAGTTTAAAGCAGATTT+GGG | + | chr7.4:76626029-76626048 | MS.gene74083:CDS | 30.0% |
| ! | TGCAAAGTTTAAAGCAGATT+TGG | + | chr7.4:76626028-76626047 | MS.gene74083:CDS | 30.0% |
| ! | TTGCTATTGGGTTCAATTAT+TGG | + | chr7.4:76626054-76626073 | MS.gene74083:CDS | 30.0% |
| AGACAACATGGTTTCACAAA+GGG | + | chr7.4:76626172-76626191 | MS.gene74083:CDS | 35.0% | |
| GATTGGAGTTGTGTAAATCA+AGG | + | chr7.4:76625898-76625917 | MS.gene74083:CDS | 35.0% | |
| GCTATTGGGTTCAATTATTG+GGG | + | chr7.4:76626056-76626075 | MS.gene74083:CDS | 35.0% | |
| GGAAAAAGTTCGATTCCATA+GGG | + | chr7.4:76626199-76626218 | MS.gene74083:CDS | 35.0% | |
| TAGACAACATGGTTTCACAA+AGG | + | chr7.4:76626171-76626190 | MS.gene74083:CDS | 35.0% | |
| TCATCACAAAGGGAAAATTG+AGG | + | chr7.4:76626244-76626263 | MS.gene74083:CDS | 35.0% | |
| TCATGAGGAATTTCATCAGT+AGG | - | chr7.4:76626293-76626312 | None:intergenic | 35.0% | |
| TGGAAAAAGTTCGATTCCAT+AGG | + | chr7.4:76626198-76626217 | MS.gene74083:CDS | 35.0% | |
| ! | AAGAATCTTGAGTGCTTTTG+TGG | - | chr7.4:76626093-76626112 | None:intergenic | 35.0% |
| ! | TGTCTTGATGATCACTCTAT+TGG | + | chr7.4:76625978-76625997 | MS.gene74083:CDS | 35.0% |
| !!! | CAGATTTGGGTTTTGCTATT+GGG | + | chr7.4:76626042-76626061 | MS.gene74083:CDS | 35.0% |
| AAAGTTCGATTCCATAGGGA+AGG | + | chr7.4:76626203-76626222 | MS.gene74083:CDS | 40.0% | |
| CATCACTTTCTTTGAATGGC+CGG | - | chr7.4:76625827-76625846 | None:intergenic | 40.0% | |
| TAGGGAAGGATTGTTCAGAA+AGG | + | chr7.4:76626217-76626236 | MS.gene74083:CDS | 40.0% | |
| TCGGCATCACTTTCTTTGAA+TGG | - | chr7.4:76625831-76625850 | None:intergenic | 40.0% | |
| TGGGAATAGATCATGAGTCA+AGG | + | chr7.4:76625793-76625812 | MS.gene74083:CDS | 40.0% | |
| TTCTGAACAATCCTTCCCTA+TGG | - | chr7.4:76626217-76626236 | None:intergenic | 40.0% | |
| TTTGCAAGTCAGGAAACTCT+TGG | - | chr7.4:76626130-76626149 | None:intergenic | 40.0% | |
| ! | AAGAGTTTCCTGACTTGCAA+AGG | + | chr7.4:76626129-76626148 | MS.gene74083:CDS | 40.0% |
| !! | GCAGATTTGGGTTTTGCTAT+TGG | + | chr7.4:76626041-76626060 | MS.gene74083:CDS | 40.0% |
| ATGAGTCAAGGATCACTCTC+CGG | + | chr7.4:76625805-76625824 | MS.gene74083:CDS | 45.0% | |
| ! | CATGGTTTCACAAAGGGTGT+TGG | + | chr7.4:76626178-76626197 | MS.gene74083:CDS | 45.0% |
| ! | GTGCTTTTGTGGCTATACCT+TGG | - | chr7.4:76626082-76626101 | None:intergenic | 45.0% |
| GGGTTCAATTATTGGGGCCA+AGG | + | chr7.4:76626062-76626081 | MS.gene74083:CDS | 50.0% | |
| TGCCCACAACAACACATCGT+CGG | - | chr7.4:76625850-76625869 | None:intergenic | 50.0% | |
| ! | ATGCCGACGATGTGTTGTTG+TGG | + | chr7.4:76625844-76625863 | MS.gene74083:CDS | 50.0% |
| ! | TCCTGACTTGCAAAGGTTGC+AGG | + | chr7.4:76626136-76626155 | MS.gene74083:CDS | 50.0% |
| ! | TGCCGACGATGTGTTGTTGT+GGG | + | chr7.4:76625845-76625864 | MS.gene74083:CDS | 50.0% |
| GCCTGCAACCTTTGCAAGTC+AGG | - | chr7.4:76626140-76626159 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 76625792 | 76626322 | 76625792 | ID=MS.gene74083 |
| chr7.4 | mRNA | 76625792 | 76626322 | 76625792 | ID=MS.gene74083.t1;Parent=MS.gene74083 |
| chr7.4 | exon | 76625792 | 76626322 | 76625792 | ID=MS.gene74083.t1.exon1;Parent=MS.gene74083.t1 |
| chr7.4 | CDS | 76625792 | 76626322 | 76625792 | ID=cds.MS.gene74083.t1;Parent=MS.gene74083.t1 |
| Gene Sequence |
| Protein sequence |
