Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74094.t1 | XP_003621906.2 | 93.4 | 137 | 9 | 0 | 1 | 137 | 34 | 170 | 4.40E-64 | 253.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74094.t1 | G7KXV9 | 93.4 | 137 | 9 | 0 | 1 | 137 | 1 | 137 | 3.2e-64 | 253.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052211 | MS.gene74094 | 0.802847 | 4.62E-49 | -1.69E-46 |
| MS.gene053406 | MS.gene74094 | 0.803285 | 3.75E-49 | -1.69E-46 |
| MS.gene055240 | MS.gene74094 | 0.800532 | 1.38E-48 | -1.69E-46 |
| MS.gene058603 | MS.gene74094 | 0.805861 | 1.09E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74094.t1 | MTR_7g024890 | 93.431 | 137 | 9 | 0 | 1 | 137 | 1 | 137 | 3.26e-90 | 258 |
| MS.gene74094.t1 | MTR_7g024880 | 93.431 | 137 | 9 | 0 | 1 | 137 | 1 | 137 | 3.34e-90 | 258 |
| MS.gene74094.t1 | MTR_7g024873 | 91.971 | 137 | 11 | 0 | 1 | 137 | 1 | 137 | 5.29e-90 | 257 |
| MS.gene74094.t1 | MTR_8g446810 | 48.571 | 140 | 69 | 1 | 1 | 137 | 1 | 140 | 5.85e-41 | 133 |
| MS.gene74094.t1 | MTR_7g024900 | 90.196 | 51 | 5 | 0 | 1 | 51 | 1 | 51 | 3.06e-26 | 93.6 |
| MS.gene74094.t1 | MTR_1g081270 | 35.878 | 131 | 79 | 1 | 1 | 131 | 1 | 126 | 2.68e-19 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74094.t1 | AT2G44310 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 4.56e-39 | 129 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTCATCTTCAAGGAAA+TGG | 0.295262 | 7.4:-76495660 | None:intergenic |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7.4:-76495823 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.342383 | 7.4:-76495666 | None:intergenic |
| CTTGCAATGGCAGATGGGTT+GGG | 0.381320 | 7.4:+76495931 | MS.gene74094:CDS |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7.4:+76495957 | MS.gene74094:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7.4:-76495961 | None:intergenic |
| TTAATAGCTTGGTAGGTAAT+CGG | 0.402120 | 7.4:+76495686 | MS.gene74094:CDS |
| CTTGAATGGCACAATTGATC+TGG | 0.437675 | 7.4:+76495876 | MS.gene74094:CDS |
| GGGTTTCATGCCAATTCAAA+TGG | 0.441784 | 7.4:+76495951 | MS.gene74094:CDS |
| TGAAGAAGCATTTAATAGCT+TGG | 0.442582 | 7.4:+76495675 | MS.gene74094:CDS |
| CTCCTTCAACATCTCTTCAT+AGG | 0.458078 | 7.4:-76495746 | None:intergenic |
| GCTTGCAATGGCAGATGGGT+TGG | 0.461788 | 7.4:+76495930 | MS.gene74094:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7.4:-76495822 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7.4:+76495762 | MS.gene74094:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7.4:+76495862 | MS.gene74094:CDS |
| CTCCTATGAAGAGATGTTGA+AGG | 0.486473 | 7.4:+76495744 | MS.gene74094:CDS |
| GAAGCATTTAATAGCTTGGT+AGG | 0.494927 | 7.4:+76495679 | MS.gene74094:CDS |
| TTTCGAAAACCCTCAAGCTC+CGG | 0.509695 | 7.4:-76495771 | None:intergenic |
| AACACACTTTGGTATTGATG+TGG | 0.520192 | 7.4:+76495792 | MS.gene74094:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7.4:+76495761 | MS.gene74094:CDS |
| AAGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7.4:+76495752 | MS.gene74094:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.550783 | 7.4:+76495925 | MS.gene74094:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7.4:+76496008 | MS.gene74094:CDS |
| GACCGTCGTTGTTTGTGTCG+AGG | 0.574792 | 7.4:-76495717 | None:intergenic |
| TTCAAGGAAATGGAAAATTG+TGG | 0.612346 | 7.4:-76495650 | None:intergenic |
| TAAGCATATGATGCTTGCAA+TGG | 0.613159 | 7.4:+76495918 | MS.gene74094:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.627241 | 7.4:+76495926 | MS.gene74094:CDS |
| CGCCTCGACACAAACAACGA+CGG | 0.676658 | 7.4:+76495715 | MS.gene74094:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAAATTTTAGA+TGG | + | chr7.4:76495625-76495644 | MS.gene74094:CDS | 25.0% |
| !! | TGAGTGTAGAAATTTTAGAT+GGG | + | chr7.4:76495626-76495645 | MS.gene74094:CDS | 25.0% |
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7.4:76495669-76495688 | None:intergenic | 30.0% | |
| TTAATAGCTTGGTAGGTAAT+CGG | + | chr7.4:76495686-76495705 | MS.gene74094:CDS | 30.0% | |
| TTCAAGGAAATGGAAAATTG+TGG | - | chr7.4:76495653-76495672 | None:intergenic | 30.0% | |
| ! | GAGTGTAGAAATTTTAGATG+GGG | + | chr7.4:76495627-76495646 | MS.gene74094:CDS | 30.0% |
| ! | TGAAAGAGAGTACAATAAAG+TGG | + | chr7.4:76496008-76496027 | MS.gene74094:CDS | 30.0% |
| !! | TGAAGAAGCATTTAATAGCT+TGG | + | chr7.4:76495675-76495694 | MS.gene74094:CDS | 30.0% |
| CTTCTTCATCTTCAAGGAAA+TGG | - | chr7.4:76495663-76495682 | None:intergenic | 35.0% | |
| TAAGCATATGATGCTTGCAA+TGG | + | chr7.4:76495918-76495937 | MS.gene74094:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | - | chr7.4:76495964-76495983 | None:intergenic | 35.0% | |
| ! | GAAGCATTTAATAGCTTGGT+AGG | + | chr7.4:76495679-76495698 | MS.gene74094:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7.4:76495792-76495811 | MS.gene74094:CDS | 35.0% |
| !! | CTCTTTCAACAGCTATTTTG+AGG | - | chr7.4:76495996-76496015 | None:intergenic | 35.0% |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7.4:76495862-76495881 | MS.gene74094:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | + | chr7.4:76495957-76495976 | MS.gene74094:CDS | 40.0% | |
| CTCCTATGAAGAGATGTTGA+AGG | + | chr7.4:76495744-76495763 | MS.gene74094:CDS | 40.0% | |
| CTCCTTCAACATCTCTTCAT+AGG | - | chr7.4:76495749-76495768 | None:intergenic | 40.0% | |
| CTTGAATGGCACAATTGATC+TGG | + | chr7.4:76495876-76495895 | MS.gene74094:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7.4:76495951-76495970 | MS.gene74094:CDS | 40.0% | |
| ! | AGGGTTTTCGAAACACACTT+TGG | + | chr7.4:76495781-76495800 | MS.gene74094:CDS | 40.0% |
| AAACACGAGCAAGCTCGTTT+GGG | - | chr7.4:76495825-76495844 | None:intergenic | 45.0% | |
| AAGAGATGTTGAAGGAGCTC+CGG | + | chr7.4:76495752-76495771 | MS.gene74094:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7.4:76495925-76495944 | MS.gene74094:CDS | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | - | chr7.4:76495826-76495845 | None:intergenic | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7.4:76495926-76495945 | MS.gene74094:CDS | 45.0% | |
| TTTCGAAAACCCTCAAGCTC+CGG | - | chr7.4:76495774-76495793 | None:intergenic | 45.0% | |
| ! | CTTGCAATGGCAGATGGGTT+GGG | + | chr7.4:76495931-76495950 | MS.gene74094:CDS | 50.0% |
| CGCCTCGACACAAACAACGA+CGG | + | chr7.4:76495715-76495734 | MS.gene74094:CDS | 55.0% | |
| GACCGTCGTTGTTTGTGTCG+AGG | - | chr7.4:76495720-76495739 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGT+TGG | + | chr7.4:76495930-76495949 | MS.gene74094:CDS | 55.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | + | chr7.4:76495762-76495781 | MS.gene74094:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | + | chr7.4:76495761-76495780 | MS.gene74094:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 76495625 | 76496038 | 76495625 | ID=MS.gene74094 |
| chr7.4 | mRNA | 76495625 | 76496038 | 76495625 | ID=MS.gene74094.t1;Parent=MS.gene74094 |
| chr7.4 | exon | 76495625 | 76496038 | 76495625 | ID=MS.gene74094.t1.exon1;Parent=MS.gene74094.t1 |
| chr7.4 | CDS | 76495625 | 76496038 | 76495625 | ID=cds.MS.gene74094.t1;Parent=MS.gene74094.t1 |
| Gene Sequence |
| Protein sequence |
