AlfalfaGEDB | MS.gene74497

M. sativa cultivar XinJiangDaYe / MS.gene74497

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12854.10 PF13041.9 PF13812.9 PF20431.1

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74497.t1	XP_003611862.1	96.1	539	21	0	1	539	125	663	9.10E-262	912.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74497.t1	Q9SJZ3	47.2	515	272	0	8	522	154	668	3.3e-95	350.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74497.t1	G7K9H7	96.1	539	21	0	1	539	125	663	6.5e-262	912.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051744	MS.gene74497	0.814431	1.55E-51	-1.69E-46
MS.gene053226	MS.gene74497	0.831179	1.93E-55	-1.69E-46
MS.gene05953	MS.gene74497	0.800266	1.56E-48	-1.69E-46
MS.gene060521	MS.gene74497	0.800527	1.38E-48	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74497.t1	MTR_5g018690	96.104	539	21	0	1	539	125	663	0.0	1085
MS.gene74497.t1	MTR_5g018690	27.869	427	242	6	32	426	55	447	2.97e-41	158
MS.gene74497.t1	MTR_5g018690	27.333	300	212	3	143	438	30	327	2.27e-24	107
MS.gene74497.t1	MTR_7g093900	41.551	503	293	1	15	516	149	651	1.61e-137	413
MS.gene74497.t1	MTR_7g093900	27.446	419	233	7	59	442	92	474	1.36e-43	164
MS.gene74497.t1	MTR_7g093900	28.531	354	180	8	8	323	243	561	2.14e-36	144
MS.gene74497.t1	MTR_7g093900	21.958	337	252	4	147	477	42	373	2.06e-19	92.4
MS.gene74497.t1	MTR_7g093900	41.767	498	289	1	15	511	99	596	6.10e-134	410
MS.gene74497.t1	MTR_7g093900	27.446	419	233	7	59	442	42	424	3.24e-43	165
MS.gene74497.t1	MTR_7g093900	28.249	354	181	7	8	323	193	511	3.49e-36	144

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74497.t1	AT2G22410	40.583	515	306	0	8	522	154	668	9.34e-141	422
MS.gene74497.t1	AT2G22410	26.764	411	234	5	66	442	108	485	5.59e-46	171
MS.gene74497.t1	AT2G29760	40.154	518	309	1	8	524	129	646	3.75e-139	420
MS.gene74497.t1	AT2G29760	27.778	396	220	7	64	426	83	445	2.22e-42	162
MS.gene74497.t1	AT2G29760	22.635	296	214	5	153	438	34	324	2.04e-19	92.4
MS.gene74497.t1	AT4G14820	37.016	516	325	0	9	524	110	625	2.90e-132	402
MS.gene74497.t1	AT2G13600	38.710	527	316	2	1	520	143	669	2.10e-127	388
MS.gene74497.t1	AT2G13600	26.556	482	250	9	32	443	42	489	3.45e-52	189
MS.gene74497.t1	AT2G13600	29.655	290	204	0	131	420	40	329	1.68e-42	162
MS.gene74497.t1	AT3G15930	37.121	528	325	2	1	524	125	649	1.57e-126	386

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Find 114 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATGGTAATTTGGATT+TGG	0.233157	5.2:+10456076	MS.gene74497:CDS
AAACAACAAAATTGCTTCTT+CGG	0.351830	5.2:-10455985	None:intergenic
AAACATGGTAATTTGGATTT+GGG	0.335265	5.2:+10456077	MS.gene74497:CDS
AAAGGGATTGTAAACTGGTT+TGG	0.405990	5.2:-10455732	None:intergenic
AACAAGTATATACTCTATGC+TGG	0.453754	5.2:+10457225	MS.gene74497:CDS
AAGATTTGTGCATCTTTATA+TGG	0.254300	5.2:+10456100	MS.gene74497:CDS
AATGGATCATCTAAAGTCTG+CGG	0.615203	5.2:+10457255	MS.gene74497:CDS
AATTGAATCAATTTCAATAC+CGG	0.471248	5.2:-10456129	None:intergenic
AATTGTTACAAATGCACTCA+TGG	0.472145	5.2:+10456148	MS.gene74497:CDS
AATTTGGTGTCATGTTCAAG+AGG	0.604857	5.2:+10456352	MS.gene74497:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAGTATATACTCTATGC+TGG	+	chr5.2:10457225-10457244	MS.gene74497:CDS	30.0%
AATTGTTACAAATGCACTCA+TGG	+	chr5.2:10456148-10456167	MS.gene74497:CDS	30.0%
ACAAGTATATACTCTATGCT+GGG	+	chr5.2:10457226-10457245	MS.gene74497:CDS	30.0%
ACATTATGAATTCGACTTTC+GGG	+	chr5.2:10456813-10456832	MS.gene74497:CDS	30.0%
ATAGACATGTATGCAAAATG+CGG	+	chr5.2:10456563-10456582	MS.gene74497:CDS	30.0%
ATCAGCTTCATTGAAATTGA+TGG	+	chr5.2:10457157-10457176	MS.gene74497:CDS	30.0%
ATGTAAACATTTGGATGTAG+AGG	+	chr5.2:10457285-10457304	MS.gene74497:CDS	30.0%
ATTAACGCTTATGCTAATCA+TGG	+	chr5.2:10456260-10456279	MS.gene74497:CDS	30.0%
CATCCTATAGAAAAGATCAA+CGG	-	chr5.2:10456393-10456412	None:intergenic	30.0%
CATCTTTATATGGTCATTAC+CGG	+	chr5.2:10456110-10456129	MS.gene74497:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	10455711	10457330	10455711	ID=MS.gene74497
chr5.2	mRNA	10455711	10457330	10455711	ID=MS.gene74497.t1;Parent=MS.gene74497
chr5.2	exon	10455711	10457330	10455711	ID=MS.gene74497.t1.exon1;Parent=MS.gene74497.t1
chr5.2	CDS	10455711	10457330	10455711	ID=cds.MS.gene74497.t1;Parent=MS.gene74497.t1

Gene Sequence

>MS.gene74497

ATGGTGGGTGATGGTCTTTTACCAAACCAGTTTACAATCCCTTTTGTTCTGAAAGCTTGTGCTGCCAAATCGTGTTATTGGGTCGGTGTTTGTGTTCATGCTCAGTCTTTTAAGCTTGGAATGGGGTCACATGCTTGTGTTCAGAACGCAATTTTGAATGTTTATGTTGCTTGTGGTTTGATAACAAGTGCAAGGAGAGTGTTTGATGATATTTCTGAAAGGACCTTGGTCTCGTGGAATTCGATGATTAATGGGTATTCTAAAATGGGTTGGTCCGAAGAAGCAATTTTGTTGTTTCGAGAAATGCAAGAAGTTGGATTGGAGCCTGATGTGTTTACGTTGGTTGGCTTGCTTTCTGTTTCGACAAAACATGGTAATTTGGATTTGGGAAGATTTGTGCATCTTTATATGGTCATTACCGGTATTGAAATTGATTCAATTGTTACAAATGCACTCATGGATATGTATGCCAAGTGTGGGAATTTGAAATGTGCTAAAAGTGTTTTTGATCAAATGCTTGATAAGGATGTTGTTTCTTGGACGTGTATGATTAACGCTTATGCTAATCATGGACTTATTGATTGTGCTTTGGAATTTTTTAACCAGATGCCGGGGAAGAACGTGGTTTCTTGGAATTCAATAATTTGGTGTCATGTTCAAGAGGGACTTTATCCGGAAGCCGTTGATCTTTTCTATAGGATGTGTGATTCAGGCGTGATGCCTAATGATGCTACTCTTGTTGCCATTCTTTCATCCTGCAGTCACATGGGTGATCTAGCATTGGGAAAACAAGCCCACAGCTACATTTTTGATAACAACATTACACTGAGTGTGACGCTTTGCAACGCTATAATAGACATGTATGCAAAATGCGGTGCTCTTCAGATTGCCATGGACGTCTTCTTCGGGATGCCTGAGAAGAATGCAGTGTCATGGAATGTTATTATCGGGGCACTTGCTCTGCATGGTTATGGAAAAGAAGCTATTGAGATGTTTGAGAAGATGCAGGCAAGTGGAGTTTGTCCTGATGAGATTACTTTTACAGGTTTGCTTTCTGCTTGTAGTCACAGTGGTCTTGTGGACATGGGGCAATATTACTTAGACATTATGAATTCGACTTTCGGGATTTCTCCTGATGTTGAACATTATGCGTGCATGGTCGATCTTTTAGGTCGCCGTGGATTGTTAGGAGAAGCGATATCATTGATTAAGAAGATGCCTGTTAAACCAGATGTTGTTGTTTGGAGTGCTTTACTTGGTGCTTGTCGAACCTATGGGAATCTAGCAATTGGTAAGCAAATCATGAAGCAGTTGTTGGAGTTGGGACGATACAATTCTGGTCTTTATGTGCTTCTCTCAAATATGTATTCAGAATCTCAAAGATGGGAAGACATGAAAAATATTTGGAAAATACTGGATCAAAATGGGATAAAAAAGTGCAGGGCAATCAGCTTCATTGAAATTGATGGCTGTTGTTATCAATTTATGGTGGATGATAAAAGACATGGAGCTTCAACAAGTATATACTCTATGCTGGGTCAATTAATGGATCATCTAAAGTCTGCGGGGTATCCATGTAAACATTTGGATGTAGAGGAAATATACTGCTCTTATATTTAA

Protein sequence

>MS.gene74497.t1

MVGDGLLPNQFTIPFVLKACAAKSCYWVGVCVHAQSFKLGMGSHACVQNAILNVYVACGLITSARRVFDDISERTLVSWNSMINGYSKMGWSEEAILLFREMQEVGLEPDVFTLVGLLSVSTKHGNLDLGRFVHLYMVITGIEIDSIVTNALMDMYAKCGNLKCAKSVFDQMLDKDVVSWTCMINAYANHGLIDCALEFFNQMPGKNVVSWNSIIWCHVQEGLYPEAVDLFYRMCDSGVMPNDATLVAILSSCSHMGDLALGKQAHSYIFDNNITLSVTLCNAIIDMYAKCGALQIAMDVFFGMPEKNAVSWNVIIGALALHGYGKEAIEMFEKMQASGVCPDEITFTGLLSACSHSGLVDMGQYYLDIMNSTFGISPDVEHYACMVDLLGRRGLLGEAISLIKKMPVKPDVVVWSALLGACRTYGNLAIGKQIMKQLLELGRYNSGLYVLLSNMYSESQRWEDMKNIWKILDQNGIKKCRAISFIEIDGCCYQFMVDDKRHGASTSIYSMLGQLMDHLKSAGYPCKHLDVEEIYCSYI