AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74526

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74526.t1 MTR_2g030480 84.399 391 19 2 1 349 1 391 0.0 662
MS.gene74526.t1 MTR_2g030470 74.696 411 42 2 1 349 416 826 0.0 611
MS.gene74526.t1 MTR_2g030470 72.927 410 49 2 2 349 5 414 0.0 599
MS.gene74526.t1 MTR_1g052640 66.019 412 77 3 1 349 1 412 0.0 538
MS.gene74526.t1 MTR_0083s0100 63.835 412 86 3 1 349 1 412 1.51e-179 504
MS.gene74526.t1 MTR_2g030460 57.524 412 112 3 1 349 1 412 1.23e-172 486
MS.gene74526.t1 MTR_4g094555 52.871 418 124 4 1 348 1 415 8.04e-151 432
MS.gene74526.t1 MTR_4g094560 55.469 384 107 3 27 348 24 405 7.06e-150 429
MS.gene74526.t1 MTR_4g094570 52.826 407 121 4 10 346 8 413 4.97e-149 427
MS.gene74526.t1 MTR_4g094548 52.745 419 125 5 1 348 1 417 1.60e-148 426
MS.gene74526.t1 MTR_6g048350 51.914 418 128 6 1 348 1 415 2.09e-146 420
MS.gene74526.t1 MTR_4g094550 51.582 411 129 3 8 348 6 416 8.08e-145 416
MS.gene74526.t1 MTR_1g052640 63.174 334 60 3 1 271 1 334 2.75e-144 412
MS.gene74526.t1 MTR_4g094562 52.346 405 122 4 8 342 6 409 3.10e-143 412
MS.gene74526.t1 MTR_6g048310 51.531 392 115 6 27 348 23 409 3.56e-139 402
MS.gene74526.t1 MTR_2g030460 53.593 334 92 3 1 271 1 334 5.82e-126 365
MS.gene74526.t1 MTR_1g052640 66.797 256 52 2 1 223 1 256 3.69e-118 343
MS.gene74526.t1 MTR_4g094565 45.550 382 128 8 1 349 1 335 2.42e-100 300
MS.gene74526.t1 MTR_6g089930 36.562 413 189 8 1 346 1 407 2.59e-92 281
MS.gene74526.t1 MTR_6g090090 36.973 403 191 9 1 346 27 423 8.89e-92 281
MS.gene74526.t1 MTR_8g039300 35.109 413 195 9 1 346 1 407 6.31e-90 275
MS.gene74526.t1 MTR_3g035040 35.323 402 189 7 12 346 10 407 3.09e-88 271
MS.gene74526.t1 MTR_4g094562 48.289 263 93 4 1 223 1 260 3.24e-78 243
MS.gene74526.t1 MTR_1g035220 37.772 368 168 11 1 317 1 358 9.02e-78 243
MS.gene74526.t1 MTR_4g094565 43.750 256 87 5 1 223 1 232 6.03e-59 193
MS.gene74526.t1 MTR_1g062750 35.784 204 84 3 60 216 1 204 6.67e-40 141
MS.gene74526.t1 MTR_3g010160 34.286 105 48 2 175 279 10 93 1.74e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74526.t1 AT5G61820 45.278 413 141 8 14 345 11 419 1.52e-113 338
MS.gene74526.t1 AT5G61820 45.278 413 141 8 14 345 11 419 1.52e-113 338

Find 66 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAACCACCTTTATAATT+TGG 0.135759 2.3:+59386202 None:intergenic
ACACTGTTAAGTGGGTTAAT+TGG 0.176457 2.3:-59386105 MS.gene74526:CDS
GGTTAATTGGGAAAAGTTTA+TGG 0.206249 2.3:-59386092 MS.gene74526:intron
TGTACTTCAATTCCAAAGTA+TGG 0.291938 2.3:-59385199 MS.gene74526:CDS
GCAATTGCATCAACTCTATA+TGG 0.293672 2.3:-59385766 MS.gene74526:CDS
CATTCTCATCAGCTGCATTT+AGG 0.294556 2.3:-59386750 MS.gene74526:CDS
CACTGTTAAGTGGGTTAATT+GGG 0.313205 2.3:-59386104 MS.gene74526:CDS
ATCTTGATAGAACCTGGTTT+AGG 0.314738 2.3:+59385118 None:intergenic
GCTGAAGTAGTTGATGAATC+AGG 0.314770 2.3:-59386595 MS.gene74526:CDS
TCTTGATAGAACCTGGTTTA+GGG 0.328959 2.3:+59385119 None:intergenic
AGGAGAGCAGGAAATGAAAA+AGG 0.329308 2.3:-59385169 MS.gene74526:CDS
TTCAAATAAAGACTTTGTTT+TGG 0.337545 2.3:+59386130 None:intergenic
AGCTTACACTTACTATTATC+AGG 0.340801 2.3:+59386172 None:intergenic
ACATATATTCCTGACCCTTA+TGG 0.358122 2.3:-59386391 MS.gene74526:CDS
CACAAACTAGGAAGCTTTCA+TGG 0.362304 2.3:-59386807 MS.gene74526:CDS
TGTCGCAAATAAAGTATTCA+TGG 0.372215 2.3:-59386662 MS.gene74526:CDS
TTTGGACAGTACTATGGTCT+TGG 0.372998 2.3:-59386436 MS.gene74526:CDS
ATCAAACTTACCTACATCAT+TGG 0.374803 2.3:-59386555 MS.gene74526:CDS
CCTGGACTGAGTTCAATCTT+AGG 0.376795 2.3:+59386688 None:intergenic
ATAAGACACTCTGGAGTAAT+GGG 0.381977 2.3:-59385049 MS.gene74526:CDS
GCAAGCTTCCCAATGCAAAA+TGG 0.392679 2.3:-59385829 MS.gene74526:CDS
GGAGTTGGTCATCAACATTC+AGG 0.394933 2.3:-59385790 MS.gene74526:CDS
ACTTTATTTGCGACAGATCC+TGG 0.397215 2.3:+59386670 None:intergenic
AATGGCGGTTATGTCATCTA+TGG 0.409553 2.3:-59385811 MS.gene74526:CDS
AGTGTTACAAAGGATGAAAA+TGG 0.419443 2.3:-59386238 MS.gene74526:CDS
TATGGAAAAGGAAGGAGAGC+AGG 0.426604 2.3:-59385181 MS.gene74526:CDS
GCTTTGAGTCCAAATTATAA+AGG 0.431464 2.3:-59386211 MS.gene74526:CDS
GTAAGTGTAAGCTGAGTAAA+GGG 0.435996 2.3:-59386162 MS.gene74526:CDS
AATGATGTAGGTAAGTTTGA+TGG 0.441509 2.3:+59386557 None:intergenic
TTGAGTGAAATATGACCTCT+TGG 0.446302 2.3:+59386628 None:intergenic
AGTAAGTGTAAGCTGAGTAA+AGG 0.446870 2.3:-59386163 MS.gene74526:CDS
TTGCCAACTTCTATGCCATA+AGG 0.454223 2.3:+59386376 None:intergenic
CATAAGACACTCTGGAGTAA+TGG 0.462758 2.3:-59385050 MS.gene74526:CDS
ATTTGAAATACACTGTTAAG+TGG 0.470589 2.3:-59386114 MS.gene74526:CDS
TCAATTCCAAAGTATGGAAA+AGG 0.471476 2.3:-59385193 MS.gene74526:CDS
AATGTCTTTGGACAGTACTA+TGG 0.480647 2.3:-59386442 MS.gene74526:CDS
CTAAATGCAGCTGATGAGAA+TGG 0.487643 2.3:+59386751 None:intergenic
GACCCTTATGGCATAGAAGT+TGG 0.502578 2.3:-59386379 MS.gene74526:CDS
AATCCTGAAGAAATTCCAAA+AGG 0.505206 2.3:-59386355 MS.gene74526:CDS
ACTTCTATGCCATAAGGGTC+AGG 0.511032 2.3:+59386382 None:intergenic
CCTAAGATTGAACTCAGTCC+AGG 0.516882 2.3:-59386688 MS.gene74526:CDS
TTCAAATAACTTGTGCTGCA+AGG 0.521800 2.3:+59385880 None:intergenic
CTGAAGTAGTTGATGAATCA+GGG 0.523027 2.3:-59386594 MS.gene74526:CDS
ATCCTGAAGAAATTCCAAAA+GGG 0.536824 2.3:-59386354 MS.gene74526:intron
TTTGAAATACACTGTTAAGT+GGG 0.542191 2.3:-59386113 MS.gene74526:CDS
TGCCAACTTCTATGCCATAA+GGG 0.542962 2.3:+59386377 None:intergenic
TTACAACAATCCAATGATGT+AGG 0.543269 2.3:+59386545 None:intergenic
TTGAGTCCAAATTATAAAGG+TGG 0.544936 2.3:-59386208 MS.gene74526:CDS
GTGACAAACAATAGTCAAGA+AGG 0.552302 2.3:-59386502 MS.gene74526:CDS
GGTTATGTCATCTATGGAGT+TGG 0.561035 2.3:-59385805 MS.gene74526:CDS
AGTAGCAACATAAGACACTC+TGG 0.563537 2.3:-59385058 MS.gene74526:CDS
TGCATCAACTCTATATGGAA+AGG 0.572778 2.3:-59385761 MS.gene74526:intron
TGAGGTTTGTATCACAAACT+AGG 0.574489 2.3:-59386819 MS.gene74526:CDS
TTGACAACAACACTAGTGCT+AGG 0.575008 2.3:+59386779 None:intergenic
TCACATATCTTGATAGAACC+TGG 0.575131 2.3:+59385112 None:intergenic
ACAAGTTATTTGAATCGTAG+TGG 0.578444 2.3:-59385871 MS.gene74526:CDS
GAATGTTAAACATGGTAATG+TGG 0.581889 2.3:+59384995 None:intergenic
TGTAGGTAAGTTTGATGGAG+AGG 0.595260 2.3:+59386562 None:intergenic
ACAAACTAGGAAGCTTTCAT+GGG 0.599787 2.3:-59386806 MS.gene74526:CDS
GGTTCTATCAAGATATGTGA+TGG 0.612437 2.3:-59385109 MS.gene74526:CDS
TTCCAAAGTATGGAAAAGGA+AGG 0.630723 2.3:-59385189 MS.gene74526:CDS
AATGATAGGAATGTTAAACA+TGG 0.639918 2.3:+59384987 None:intergenic
GGTCTTGGATCTGAAACAAG+AGG 0.643139 2.3:-59386421 MS.gene74526:CDS
TGATTTGTATAGTGTTACAA+AGG 0.644090 2.3:-59386248 MS.gene74526:intron
AGCTTCCCAATGCAAAATGG+CGG 0.662013 2.3:-59385826 MS.gene74526:CDS
TCAACATGTTACCCTAAACC+AGG 0.665969 2.3:-59385130 MS.gene74526:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAATTATTTTTATACAAT+TGG + chr2.3:59386386-59386405 None:intergenic 10.0%
!!! ATTATTTTTAAAAGAAACAA+TGG - chr2.3:59385342-59385361 MS.gene74526:intron 10.0%
!! CATATATAGTCTAATTATAA+AGG + chr2.3:59386484-59386503 None:intergenic 15.0%
!!! CAGAACTTTTTTTATTTTTA+AGG + chr2.3:59386157-59386176 None:intergenic 15.0%
!!! TTTTGTTTGAATACTTTTTT+TGG + chr2.3:59385100-59385119 None:intergenic 15.0%
!! TCAAATATATAAATCTTGAG+AGG + chr2.3:59385756-59385775 None:intergenic 20.0%
!!! TTCAAATAAAGACTTTGTTT+TGG + chr2.3:59385693-59385712 None:intergenic 20.0%
!!! TTTTACCACAAATTTTTAGA+TGG - chr2.3:59386203-59386222 MS.gene74526:CDS 20.0%
! AATAGATTGTTAATTGTCCT+TGG + chr2.3:59386316-59386335 None:intergenic 25.0%
! AATGAAAAAGGATATGTTGT+AGG - chr2.3:59386663-59386682 MS.gene74526:CDS 25.0%
! ATATTCATGCTATTGATACT+AGG - chr2.3:59385511-59385530 MS.gene74526:intron 25.0%
! GTTAACCATCTAAAAATTTG+TGG + chr2.3:59386211-59386230 None:intergenic 25.0%
! TATTCATGCTATTGATACTA+GGG - chr2.3:59385512-59385531 MS.gene74526:intron 25.0%
!! ATTTGAAATACACTGTTAAG+TGG - chr2.3:59385706-59385725 MS.gene74526:intron 25.0%
!! CTATTGTTTGTCACATTTTT+AGG + chr2.3:59385312-59385331 None:intergenic 25.0%
!! TGATTTGTATAGTGTTACAA+AGG - chr2.3:59385572-59385591 MS.gene74526:intron 25.0%
!! TTTGAAATACACTGTTAAGT+GGG - chr2.3:59385707-59385726 MS.gene74526:intron 25.0%
!!! GGATCAAAGTTTTGAAATTT+TGG - chr2.3:59386565-59386584 MS.gene74526:CDS 25.0%
AAAGATCAAGACGTCTAATA+TGG + chr2.3:59386444-59386463 None:intergenic 30.0%
AAGATCAAGACGTCTAATAT+GGG + chr2.3:59386443-59386462 None:intergenic 30.0%
AAGATTTCAGATAAGTCCAA+AGG - chr2.3:59385792-59385811 MS.gene74526:CDS 30.0%
AATCCTGAAGAAATTCCAAA+AGG - chr2.3:59385465-59385484 MS.gene74526:intron 30.0%
AATGATGTAGGTAAGTTTGA+TGG + chr2.3:59385266-59385285 None:intergenic 30.0%
ACAAGTTATTTGAATCGTAG+TGG - chr2.3:59385949-59385968 MS.gene74526:intron 30.0%
AGCTTACACTTACTATTATC+AGG + chr2.3:59385651-59385670 None:intergenic 30.0%
AGTGTTACAAAGGATGAAAA+TGG - chr2.3:59385582-59385601 MS.gene74526:intron 30.0%
ATCAAACTTACCTACATCAT+TGG - chr2.3:59385265-59385284 MS.gene74526:intron 30.0%
ATCCTGAAGAAATTCCAAAA+GGG - chr2.3:59385466-59385485 MS.gene74526:intron 30.0%
ATGGTTAACGAAGTACAAAT+AGG - chr2.3:59386222-59386241 MS.gene74526:CDS 30.0%
ATTCATGCTATTGATACTAG+GGG - chr2.3:59385513-59385532 MS.gene74526:intron 30.0%
GGTTAATTGGGAAAAGTTTA+TGG - chr2.3:59385728-59385747 MS.gene74526:intron 30.0%
TCAATTCCAAAGTATGGAAA+AGG - chr2.3:59386627-59386646 MS.gene74526:CDS 30.0%
TGCAAACCACCTTTATAATT+TGG + chr2.3:59385621-59385640 None:intergenic 30.0%
TGTACTTCAATTCCAAAGTA+TGG - chr2.3:59386621-59386640 MS.gene74526:CDS 30.0%
TTACAACAATCCAATGATGT+AGG + chr2.3:59385278-59385297 None:intergenic 30.0%
TTGAGTCCAAATTATAAAGG+TGG - chr2.3:59385612-59385631 MS.gene74526:intron 30.0%
TTTCTCTTAATCATCTACCA+AGG - chr2.3:59386296-59386315 MS.gene74526:intron 30.0%
! GCTTTGAGTCCAAATTATAA+AGG - chr2.3:59385609-59385628 MS.gene74526:intron 30.0%
! TGTCGCAAATAAAGTATTCA+TGG - chr2.3:59385158-59385177 MS.gene74526:CDS 30.0%
! TTTTGTCAAGAGAATGTCTT+TGG - chr2.3:59385366-59385385 MS.gene74526:intron 30.0%
!! AATGGGACTTTTCTATTTCT+TGG - chr2.3:59386788-59386807 MS.gene74526:CDS 30.0%
!!! TGTTTGTCACATTTTTAGGT+TGG + chr2.3:59385308-59385327 None:intergenic 30.0%
AATGTCTTTGGACAGTACTA+TGG - chr2.3:59385378-59385397 MS.gene74526:intron 35.0%
ACAAACTAGGAAGCTTTCAT+GGG - chr2.3:59385014-59385033 MS.gene74526:CDS 35.0%
ACATATATTCCTGACCCTTA+TGG - chr2.3:59385429-59385448 MS.gene74526:intron 35.0%
AGATCAAGACGTCTAATATG+GGG + chr2.3:59386442-59386461 None:intergenic 35.0%
AGTAAGTGTAAGCTGAGTAA+AGG - chr2.3:59385657-59385676 MS.gene74526:intron 35.0%
ATCTTGATAGAACCTGGTTT+AGG + chr2.3:59386705-59386724 None:intergenic 35.0%
ATGGTTTAGTCTCTTTCCTT+TGG + chr2.3:59385811-59385830 None:intergenic 35.0%
GCAATTGCATCAACTCTATA+TGG - chr2.3:59386054-59386073 MS.gene74526:intron 35.0%
GGTTCTATCAAGATATGTGA+TGG - chr2.3:59386711-59386730 MS.gene74526:CDS 35.0%
GTAAGTGTAAGCTGAGTAAA+GGG - chr2.3:59385658-59385677 MS.gene74526:intron 35.0%
GTGACAAACAATAGTCAAGA+AGG - chr2.3:59385318-59385337 MS.gene74526:intron 35.0%
TCACATATCTTGATAGAACC+TGG + chr2.3:59386711-59386730 None:intergenic 35.0%
TCTTGATAGAACCTGGTTTA+GGG + chr2.3:59386704-59386723 None:intergenic 35.0%
TGAGGTTTGTATCACAAACT+AGG - chr2.3:59385001-59385020 MS.gene74526:CDS 35.0%
TGCATCAACTCTATATGGAA+AGG - chr2.3:59386059-59386078 MS.gene74526:intron 35.0%
TTCCAAAGTATGGAAAAGGA+AGG - chr2.3:59386631-59386650 MS.gene74526:CDS 35.0%
TTGAGTGAAATATGACCTCT+TGG + chr2.3:59385195-59385214 None:intergenic 35.0%
TTGGTTGTGACATTAATGTC+AGG - chr2.3:59386584-59386603 MS.gene74526:CDS 35.0%
TTGTGACATTAATGTCAGGA+TGG - chr2.3:59386588-59386607 MS.gene74526:CDS 35.0%
! AAGCTGAGTAAAGGGTTTTT+AGG - chr2.3:59385666-59385685 MS.gene74526:intron 35.0%
! AGAAATTCCAAAAGGGTATG+TGG - chr2.3:59385473-59385492 MS.gene74526:intron 35.0%
! CAAAAGGGTATGTGGAAAAA+TGG - chr2.3:59385481-59385500 MS.gene74526:intron 35.0%
! CATTTTTCCACATACCCTTT+TGG + chr2.3:59385483-59385502 None:intergenic 35.0%
! TTCAAATAACTTGTGCTGCA+AGG + chr2.3:59385943-59385962 None:intergenic 35.0%
!! ACACTGTTAAGTGGGTTAAT+TGG - chr2.3:59385715-59385734 MS.gene74526:intron 35.0%
!! ATAAGACACTCTGGAGTAAT+GGG - chr2.3:59386771-59386790 MS.gene74526:CDS 35.0%
!! CACTGTTAAGTGGGTTAATT+GGG - chr2.3:59385716-59385735 MS.gene74526:intron 35.0%
!! CTGAAGTAGTTGATGAATCA+GGG - chr2.3:59385226-59385245 MS.gene74526:intron 35.0%
!!! ATGGATGTTGATTTTCCAAG+AGG - chr2.3:59385177-59385196 MS.gene74526:CDS 35.0%
!!! TACCCTTTTGGAATTTCTTC+AGG + chr2.3:59385471-59385490 None:intergenic 35.0%
!!! TTGTCACATTTTTAGGTTGG+TGG + chr2.3:59385305-59385324 None:intergenic 35.0%
AATGGCGGTTATGTCATCTA+TGG - chr2.3:59386009-59386028 MS.gene74526:intron 40.0%
ACTACTACTCTCTAGTGACA+TGG - chr2.3:59386121-59386140 MS.gene74526:CDS 40.0%
AGGAGAGCAGGAAATGAAAA+AGG - chr2.3:59386651-59386670 MS.gene74526:CDS 40.0%
AGTAGCAACATAAGACACTC+TGG - chr2.3:59386762-59386781 MS.gene74526:CDS 40.0%
CACAAACTAGGAAGCTTTCA+TGG - chr2.3:59385013-59385032 MS.gene74526:CDS 40.0%
CATTCTCATCAGCTGCATTT+AGG - chr2.3:59385070-59385089 MS.gene74526:CDS 40.0%
CTAAACCATGCATGATTGCA+AGG - chr2.3:59385822-59385841 MS.gene74526:CDS 40.0%
CTAAATGCAGCTGATGAGAA+TGG + chr2.3:59385072-59385091 None:intergenic 40.0%
GATCAAGACGTCTAATATGG+GGG + chr2.3:59386441-59386460 None:intergenic 40.0%
GGTTATGTCATCTATGGAGT+TGG - chr2.3:59386015-59386034 MS.gene74526:intron 40.0%
TCAACATGTTACCCTAAACC+AGG - chr2.3:59386690-59386709 MS.gene74526:CDS 40.0%
TGCCAACTTCTATGCCATAA+GGG + chr2.3:59385446-59385465 None:intergenic 40.0%
TGTAGGTAAGTTTGATGGAG+AGG + chr2.3:59385261-59385280 None:intergenic 40.0%
TTGACAACAACACTAGTGCT+AGG + chr2.3:59385044-59385063 None:intergenic 40.0%
TTGCCAACTTCTATGCCATA+AGG + chr2.3:59385447-59385466 None:intergenic 40.0%
TTTGGACAGTACTATGGTCT+TGG - chr2.3:59385384-59385403 MS.gene74526:intron 40.0%
! ACTTTATTTGCGACAGATCC+TGG + chr2.3:59385153-59385172 None:intergenic 40.0%
! CTCCTTCCTTTTCCATACTT+TGG + chr2.3:59386636-59386655 None:intergenic 40.0%
!! ACATAACCGCCATTTTGCAT+TGG + chr2.3:59386003-59386022 None:intergenic 40.0%
!! CATAACCGCCATTTTGCATT+GGG + chr2.3:59386002-59386021 None:intergenic 40.0%
!! CATAAGACACTCTGGAGTAA+TGG - chr2.3:59386770-59386789 MS.gene74526:CDS 40.0%
!! GCTGAAGTAGTTGATGAATC+AGG - chr2.3:59385225-59385244 MS.gene74526:intron 40.0%
!! GGGACTTTTCTATTTCTTGG+TGG - chr2.3:59386791-59386810 MS.gene74526:CDS 40.0%
ACTTCTATGCCATAAGGGTC+AGG + chr2.3:59385441-59385460 None:intergenic 45.0%
AGCTTCCCAATGCAAAATGG+CGG - chr2.3:59385994-59386013 MS.gene74526:intron 45.0%
CCTAAGATTGAACTCAGTCC+AGG - chr2.3:59385132-59385151 MS.gene74526:CDS 45.0%
CCTGGACTGAGTTCAATCTT+AGG + chr2.3:59385135-59385154 None:intergenic 45.0%
GACCCTTATGGCATAGAAGT+TGG - chr2.3:59385441-59385460 MS.gene74526:intron 45.0%
GCAAGCTTCCCAATGCAAAA+TGG - chr2.3:59385991-59386010 MS.gene74526:intron 45.0%
GCTTACCTTGCAATCATGCA+TGG + chr2.3:59385830-59385849 None:intergenic 45.0%
TATGGAAAAGGAAGGAGAGC+AGG - chr2.3:59386639-59386658 MS.gene74526:CDS 45.0%
TGCTATTGATACTAGGGGTG+TGG - chr2.3:59385518-59385537 MS.gene74526:intron 45.0%
! AGGGGTGTGGAAGATAGAAT+GGG - chr2.3:59385531-59385550 MS.gene74526:intron 45.0%
! GGAGTTGGTCATCAACATTC+AGG - chr2.3:59386030-59386049 MS.gene74526:intron 45.0%
! GGTCTTGGATCTGAAACAAG+AGG - chr2.3:59385399-59385418 MS.gene74526:intron 45.0%
! TAGGGGTGTGGAAGATAGAA+TGG - chr2.3:59385530-59385549 MS.gene74526:intron 45.0%
!!! TTATCATTTTAATTAAATAT+TGG - chr2.3:59386544-59386563 MS.gene74526:CDS 5.0%
GGGGTGTGGAAGATAGAATG+GGG - chr2.3:59385532-59385551 MS.gene74526:intron 50.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 59385000 59386842 59385000 ID=MS.gene74526
chr2.3 mRNA 59385000 59386842 59385000 ID=MS.gene74526.t1;Parent=MS.gene74526
chr2.3 exon 59386355 59386842 59386355 ID=MS.gene74526.t1.exon1;Parent=MS.gene74526.t1
chr2.3 CDS 59386355 59386842 59386355 ID=cds.MS.gene74526.t1;Parent=MS.gene74526.t1
chr2.3 exon 59386093 59386258 59386093 ID=MS.gene74526.t1.exon2;Parent=MS.gene74526.t1
chr2.3 CDS 59386093 59386258 59386093 ID=cds.MS.gene74526.t1;Parent=MS.gene74526.t1
chr2.3 exon 59385762 59385920 59385762 ID=MS.gene74526.t1.exon3;Parent=MS.gene74526.t1
chr2.3 CDS 59385762 59385920 59385762 ID=cds.MS.gene74526.t1;Parent=MS.gene74526.t1
chr2.3 exon 59385000 59385236 59385000 ID=MS.gene74526.t1.exon4;Parent=MS.gene74526.t1
chr2.3 CDS 59385000 59385236 59385000 ID=cds.MS.gene74526.t1;Parent=MS.gene74526.t1
Gene Sequence

>MS.gene74526

ATGAGGTTTGTATCACAAACTAGGAAGCTTTCATGGGCAATCCTAGCACTAGTGTTGTTGTCAAACATACCATTCTCATCAGCTGCATTTAGGAAGACCAAAAAAAGTATTCAAACAAAAGTTTTCTTCTCACCTAAGATTGAACTCAGTCCAGGATCTGTCGCAAATAAAGTATTCATGGATGTTGATTTTCCAAGAGGTCATATTTCACTCAAGAGTTTCTTTGCTGAAGTAGTTGATGAATCAGGGAACTCAATACCTCTCCATCAAACTTACCTACATCATTGGATTGTTGTAAGATACCACCAACCTAAAAATGTGACAAACAATAGTCAAGAAGGTATTATTTTTAAAAGAAACAATGGATTTTGTCAAGAGAATGTCTTTGGACAGTACTATGGTCTTGGATCTGAAACAAGAGGCACAAATACATATATTCCTGACCCTTATGGCATAGAAGTTGGCAATCCTGAAGAAATTCCAAAAGGGTATGTGGAAAAATGGTTTATAAATATTCATGCTATTGATACTAGGGGTGTGGAAGATAGAATGGGGTGTACTGAGTGTAAGTGTGATTTGTATAGTGTTACAAAGGATGAAAATGGAGAAGCTTTGAGTCCAAATTATAAAGGTGGTTTGCAATGTTGTCCTGATAATAGTAAGTGTAAGCTGAGTAAAGGGTTTTTAGGACCAAAACAAAGTCTTTATTTGAAATACACTGTTAAGTGGGTTAATTGGGAAAAGTTTATGGTACCTCTCAAGATTTATATATTTGATGTGACTGATACTTTGAAGATTTCAGATAAGTCCAAAGGAAAGAGACTAAACCATGCATGATTGCAAGGTAAGCTAAATCAAAATATAATTTATATAAAATTCTATTTCATGTGTGAGTAACTGAGATTCTTTGCTATGTGTACAGCTTGAATATGAAGTAGAACCTTGCAGCACAAGTTATTTGAATCGTAGTGGTTGTGTTGATGTCAAGAGAGCAAGCTTCCCAATGCAAAATGGCGGTTATGTCATCTATGGAGTTGGTCATCAACATTCAGGTGCAATTGCATCAACTCTATATGGAAAGGTGAACATTAAATAATTGCAATCATTGTTAATATTTGAATACTACTACTCTCTAGTGACATGGTGCATACATACCTTAAAAATAAAAAAAGTTCTGTTATTTAAAAAATTAAATTGTTCAACTTTTACCACAAATTTTTAGATGGTTAACGAAGTACAAATAGGTAGTTAACTATATTATCATGAATGATAAAGTTGTGTTGACACTTGATAAGTTTTCTCTTAATCATCTACCAAGGACAATTAACAATCTATTTGAACTTTGTTACTTTATTAAGAAGTTGTCACAAAAGTTGTCTAAAACCAATTGTATAAAAATAATTTCTCAAAATAAAAATGTTTTCTAAATTTGCAGTTTCCCCCATATTAGACGTCTTGATCTTTTGTATTAATTTGATCAACCTTTATAATTAGACTATATATGCTACTGTTACTTCGTTTGATTACTTATACTCTTTGTACTCTTATCATTTTAATTAAATATTGGATCAAAGTTTTGAAATTTTGGTTGTGACATTAATGTCAGGATGGAAGAGTTATATGTACTTCAATTCCAAAGTATGGAAAAGGAAGGAGAGCAGGAAATGAAAAAGGATATGTTGTAGGAATGTCAACATGTTACCCTAAACCAGGTTCTATCAAGATATGTGATGGTGAAACTTTAACATTAGAGACTAACTACAGTAGCAACATAAGACACTCTGGAGTAATGGGACTTTTCTATTTCTTGGTGGCAGAAAAGCTACCACATTACCATGTTTAA
Protein sequence

>MS.gene74526.t1

MRFVSQTRKLSWAILALVLLSNIPFSSAAFRKTKKSIQTKVFFSPKIELSPGSVANKVFMDVDFPRGHISLKSFFAEVVDESGNSIPLHQTYLHHWIVVRYHQPKNVTNNSQEGIIFKRNNGFCQENVFGQYYGLGSETRGTNTYIPDPYGIEVGNPEEIPKGVTKDENGEALSPNYKGGLQCCPDNSKCKLSKGFLGPKQSLYLKYTVKWVNWEKFMLEYEVEPCSTSYLNRSGCVDVKRASFPMQNGGYVIYGVGHQHSGAIASTLYGKDGRVICTSIPKYGKGRRAGNEKGYVVGMSTCYPKPGSIKICDGETLTLETNYSSNIRHSGVMGLFYFLVAEKLPHYHV