Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74799.t1 | AFK36374.1 | 80.3 | 228 | 5 | 2 | 1 | 188 | 49 | 276 | 7.90E-96 | 359.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74799.t1 | Q9FZ62 | 46.4 | 211 | 71 | 4 | 1 | 169 | 52 | 262 | 7.1e-45 | 181.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74799.t1 | B7FI56 | 80.3 | 228 | 5 | 2 | 1 | 188 | 49 | 276 | 5.7e-96 | 359.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050640 | MS.gene74799 | 0.821194 | 4.60E-53 | -1.69E-46 |
| MS.gene055217 | MS.gene74799 | 0.82854 | 8.49E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74799.t1 | MTR_2g100340 | 80.263 | 228 | 5 | 2 | 1 | 188 | 49 | 276 | 1.26e-123 | 350 |
| MS.gene74799.t1 | MTR_8g015910 | 47.345 | 226 | 75 | 3 | 1 | 186 | 50 | 271 | 9.51e-63 | 195 |
| MS.gene74799.t1 | MTR_8g015960 | 45.815 | 227 | 78 | 4 | 1 | 186 | 50 | 272 | 5.89e-60 | 188 |
| MS.gene74799.t1 | MTR_2g100350 | 45.778 | 225 | 79 | 3 | 1 | 186 | 49 | 269 | 1.43e-58 | 184 |
| MS.gene74799.t1 | MTR_8g015950 | 44.934 | 227 | 80 | 5 | 1 | 186 | 50 | 272 | 1.12e-57 | 182 |
| MS.gene74799.t1 | MTR_2g062320 | 45.575 | 226 | 79 | 3 | 1 | 186 | 49 | 270 | 2.48e-57 | 181 |
| MS.gene74799.t1 | MTR_8g015940 | 45.794 | 214 | 72 | 3 | 1 | 174 | 50 | 259 | 2.53e-57 | 181 |
| MS.gene74799.t1 | MTR_8g101690 | 41.667 | 192 | 70 | 4 | 1 | 151 | 48 | 238 | 1.86e-43 | 145 |
| MS.gene74799.t1 | MTR_2g100345 | 73.846 | 65 | 17 | 0 | 123 | 187 | 13 | 77 | 4.34e-28 | 100 |
| MS.gene74799.t1 | MTR_8g040160 | 40.367 | 109 | 53 | 4 | 28 | 127 | 1 | 106 | 1.41e-17 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74799.t1 | AT1G17710 | 49.474 | 190 | 55 | 3 | 1 | 149 | 20 | 209 | 1.24e-56 | 179 |
| MS.gene74799.t1 | AT1G17710 | 49.474 | 190 | 55 | 3 | 1 | 149 | 52 | 241 | 4.06e-56 | 179 |
| MS.gene74799.t1 | AT1G73010 | 44.444 | 216 | 78 | 3 | 1 | 174 | 59 | 274 | 8.37e-52 | 168 |
| MS.gene74799.t1 | AT4G29530 | 39.153 | 189 | 74 | 3 | 1 | 148 | 38 | 226 | 2.72e-37 | 129 |
| MS.gene74799.t1 | AT4G29530 | 39.153 | 189 | 74 | 3 | 1 | 148 | 49 | 237 | 7.15e-37 | 128 |
Find 33 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATTGCCTTGCACATATT+TGG | 0.255407 | 7.1:+85598473 | None:intergenic |
| TTAGGAACTGATAGAGCTTC+AGG | 0.281232 | 7.1:+85597620 | None:intergenic |
| AAATTGTAGAAGTTCTTAAT+AGG | 0.333392 | 7.1:-85598902 | MS.gene74799:CDS |
| ATCATCAACAAAGCTAATAT+TGG | 0.348472 | 7.1:-85597725 | MS.gene74799:CDS |
| ATTGAGGCTGCTTATTCTCT+AGG | 0.365751 | 7.1:-85598849 | MS.gene74799:CDS |
| CAAAATTCACTTGATGATGA+AGG | 0.378739 | 7.1:-85597942 | MS.gene74799:CDS |
| GTGATAATGCGAGGATGAAT+TGG | 0.382658 | 7.1:+85598876 | None:intergenic |
| AACAAAGCTAATATTGGTAA+AGG | 0.419483 | 7.1:-85597719 | MS.gene74799:CDS |
| ATCTCAGTTGATTGCAAGAT+GGG | 0.430587 | 7.1:-85597674 | MS.gene74799:CDS |
| AAGAAGAAAATCATCTACTT+AGG | 0.467585 | 7.1:-85597918 | MS.gene74799:CDS |
| ACAAAGCTAATATTGGTAAA+GGG | 0.467952 | 7.1:-85597718 | MS.gene74799:CDS |
| ATTGGTAAAGGGAACATTGT+TGG | 0.470539 | 7.1:-85597707 | MS.gene74799:CDS |
| CATGTACATGGTTGGAGAGA+TGG | 0.479326 | 7.1:-85597776 | MS.gene74799:CDS |
| TAGGAACTGATAGAGCTTCA+GGG | 0.502197 | 7.1:+85597621 | None:intergenic |
| TAGAGAATAAGCAGCCTCAA+TGG | 0.509736 | 7.1:+85598851 | None:intergenic |
| TTGAGGCTGCTTATTCTCTA+GGG | 0.512196 | 7.1:-85598848 | MS.gene74799:intron |
| AAAATCATCTACTTAGGAGA+TGG | 0.512607 | 7.1:-85597912 | MS.gene74799:CDS |
| CATCTCAGTTGATTGCAAGA+TGG | 0.524133 | 7.1:-85597675 | MS.gene74799:CDS |
| GATTATCTAAAATCTTCTCA+TGG | 0.538879 | 7.1:-85598512 | MS.gene74799:CDS |
| CTCACTTTGAGCTTTAGACT+AGG | 0.544849 | 7.1:+85597873 | None:intergenic |
| GACATAAAGGCACATGTACA+TGG | 0.547678 | 7.1:-85597788 | MS.gene74799:intron |
| TGCACATATTTGGTGGACAA+AGG | 0.560853 | 7.1:+85598483 | None:intergenic |
| TAAAGGCACATGTACATGGT+TGG | 0.564702 | 7.1:-85597784 | MS.gene74799:intron |
| TGACATACAGGGAAAGGTGA+TGG | 0.566260 | 7.1:-85597973 | MS.gene74799:intron |
| ATCTACTTAGGAGATGGAAG+TGG | 0.574030 | 7.1:-85597906 | MS.gene74799:CDS |
| ATTGCCTTGCACATATTTGG+TGG | 0.577663 | 7.1:+85598476 | None:intergenic |
| TCGCATTATCACTGCCATTG+AGG | 0.620838 | 7.1:-85598865 | MS.gene74799:CDS |
| TTGTCCACCAAATATGTGCA+AGG | 0.634563 | 7.1:-85598480 | MS.gene74799:intron |
| ATGAATGAACTTCATGCACA+AGG | 0.639690 | 7.1:-85598939 | MS.gene74799:CDS |
| TTGTGTTCAGGTGTGAGCTG+AGG | 0.644223 | 7.1:-85598670 | MS.gene74799:intron |
| ACATGGTTGGAGAGATGGAG+AGG | 0.648554 | 7.1:-85597771 | MS.gene74799:CDS |
| TCTCAGTTGATTGCAAGATG+GGG | 0.664938 | 7.1:-85597673 | MS.gene74799:CDS |
| TCAATGGCAGTGATAATGCG+AGG | 0.688273 | 7.1:+85598867 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAATTCAAATGACATAA+AGG | - | chr7.1:85598757-85598776 | MS.gene74799:intron | 15.0% |
| !! | TAGTATGAGAATAATTTATA+CGG | - | chr7.1:85597780-85597799 | MS.gene74799:CDS | 15.0% |
| !!! | TAAGATGTAATTTTATGTAT+AGG | + | chr7.1:85598508-85598527 | None:intergenic | 15.0% |
| !! | AAATTGTAGAAGTTCTTAAT+AGG | - | chr7.1:85597656-85597675 | MS.gene74799:CDS | 20.0% |
| !! | ATGAATATAATAGCGTTATA+TGG | + | chr7.1:85598134-85598153 | None:intergenic | 20.0% |
| !! | TCATCAACAATATATAGAAA+CGG | + | chr7.1:85598227-85598246 | None:intergenic | 20.0% |
| ! | AACAAAGCTAATATTGGTAA+AGG | - | chr7.1:85598839-85598858 | MS.gene74799:intron | 25.0% |
| ! | AAGAAGAAAATCATCTACTT+AGG | - | chr7.1:85598640-85598659 | MS.gene74799:CDS | 25.0% |
| ! | AAGATGAAATATTTGTGTTC+AGG | - | chr7.1:85597876-85597895 | MS.gene74799:intron | 25.0% |
| ! | ACAAAGCTAATATTGGTAAA+GGG | - | chr7.1:85598840-85598859 | MS.gene74799:intron | 25.0% |
| ! | ATCATCAACAAAGCTAATAT+TGG | - | chr7.1:85598833-85598852 | MS.gene74799:intron | 25.0% |
| ! | ATGGTCATAAATAGATTTAG+AGG | + | chr7.1:85598115-85598134 | None:intergenic | 25.0% |
| ! | TAGAAACGGTGAATAAAATA+TGG | + | chr7.1:85598213-85598232 | None:intergenic | 25.0% |
| !! | GAGAAGATTTTAGATAATCA+TGG | + | chr7.1:85598045-85598064 | None:intergenic | 25.0% |
| !! | GATTATCTAAAATCTTCTCA+TGG | - | chr7.1:85598046-85598065 | MS.gene74799:intron | 25.0% |
| !!! | AAACAATTTTGAAGCATCAT+GGG | - | chr7.1:85597942-85597961 | MS.gene74799:CDS | 25.0% |
| AAAATCATCTACTTAGGAGA+TGG | - | chr7.1:85598646-85598665 | MS.gene74799:CDS | 30.0% | |
| CAAGTTATGTTAGTGAAGAA+GGG | - | chr7.1:85597999-85598018 | MS.gene74799:intron | 30.0% | |
| CTTCTTCACTAACATAACTT+GGG | + | chr7.1:85598000-85598019 | None:intergenic | 30.0% | |
| ! | AGATTTTAGATAATCATGGC+AGG | + | chr7.1:85598041-85598060 | None:intergenic | 30.0% |
| ! | CAATCAACTGAGATGATTTT+TGG | + | chr7.1:85598878-85598897 | None:intergenic | 30.0% |
| ! | GATTTTAGATAATCATGGCA+GGG | + | chr7.1:85598040-85598059 | None:intergenic | 30.0% |
| !! | CAAAATTCACTTGATGATGA+AGG | - | chr7.1:85598616-85598635 | MS.gene74799:CDS | 30.0% |
| !!! | AACAATTTTGAAGCATCATG+GGG | - | chr7.1:85597943-85597962 | MS.gene74799:CDS | 30.0% |
| !!! | GAAACAATTTTGAAGCATCA+TGG | - | chr7.1:85597941-85597960 | MS.gene74799:CDS | 30.0% |
| AGAATTCTGCCTACACAAAA+AGG | - | chr7.1:85598398-85598417 | MS.gene74799:intron | 35.0% | |
| ATCTCAGTTGATTGCAAGAT+GGG | - | chr7.1:85598884-85598903 | MS.gene74799:CDS | 35.0% | |
| ATGAATGAACTTCATGCACA+AGG | - | chr7.1:85597619-85597638 | MS.gene74799:CDS | 35.0% | |
| ATTGGTAAAGGGAACATTGT+TGG | - | chr7.1:85598851-85598870 | MS.gene74799:CDS | 35.0% | |
| CCAAGTTATGTTAGTGAAGA+AGG | - | chr7.1:85597998-85598017 | MS.gene74799:intron | 35.0% | |
| CCTTCTTCACTAACATAACT+TGG | + | chr7.1:85598001-85598020 | None:intergenic | 35.0% | |
| TCATGCACAAGGAAAAACAA+TGG | - | chr7.1:85597630-85597649 | MS.gene74799:CDS | 35.0% | |
| ! | ACGATTTTGCCTTTTTGTGT+AGG | + | chr7.1:85598410-85598429 | None:intergenic | 35.0% |
| !! | TTGTTGATGACTAATGAACG+TGG | - | chr7.1:85598237-85598256 | MS.gene74799:intron | 35.0% |
| ATCTACTTAGGAGATGGAAG+TGG | - | chr7.1:85598652-85598671 | MS.gene74799:CDS | 40.0% | |
| ATTGAGGCTGCTTATTCTCT+AGG | - | chr7.1:85597709-85597728 | MS.gene74799:CDS | 40.0% | |
| ATTGCCTTGCACATATTTGG+TGG | + | chr7.1:85598085-85598104 | None:intergenic | 40.0% | |
| CATCTCAGTTGATTGCAAGA+TGG | - | chr7.1:85598883-85598902 | MS.gene74799:CDS | 40.0% | |
| GCAATTGCCTTGCACATATT+TGG | + | chr7.1:85598088-85598107 | None:intergenic | 40.0% | |
| GTTATGTTAGTGAAGAAGGG+AGG | - | chr7.1:85598002-85598021 | MS.gene74799:intron | 40.0% | |
| TAGAGAATAAGCAGCCTCAA+TGG | + | chr7.1:85597710-85597729 | None:intergenic | 40.0% | |
| TAGGAACTGATAGAGCTTCA+GGG | + | chr7.1:85598940-85598959 | None:intergenic | 40.0% | |
| TCTCAGTTGATTGCAAGATG+GGG | - | chr7.1:85598885-85598904 | MS.gene74799:CDS | 40.0% | |
| TTAGGAACTGATAGAGCTTC+AGG | + | chr7.1:85598941-85598960 | None:intergenic | 40.0% | |
| TTGAGGCTGCTTATTCTCTA+GGG | - | chr7.1:85597710-85597729 | MS.gene74799:CDS | 40.0% | |
| TTGTCCACCAAATATGTGCA+AGG | - | chr7.1:85598078-85598097 | MS.gene74799:intron | 40.0% | |
| TTTCCCTGTATGTCATTCGA+GGG | + | chr7.1:85598580-85598599 | None:intergenic | 40.0% | |
| ! | GACATAAAGGCACATGTACA+TGG | - | chr7.1:85598770-85598789 | MS.gene74799:intron | 40.0% |
| ! | GTGATAATGCGAGGATGAAT+TGG | + | chr7.1:85597685-85597704 | None:intergenic | 40.0% |
| ! | TAAAGGCACATGTACATGGT+TGG | - | chr7.1:85598774-85598793 | MS.gene74799:intron | 40.0% |
| ! | TGCACATATTTGGTGGACAA+AGG | + | chr7.1:85598078-85598097 | None:intergenic | 40.0% |
| ! | TTGACCAGATTTTCCTCCAA+AGG | + | chr7.1:85598267-85598286 | None:intergenic | 40.0% |
| !! | CTCACTTTGAGCTTTAGACT+AGG | + | chr7.1:85598688-85598707 | None:intergenic | 40.0% |
| CATGTACATGGTTGGAGAGA+TGG | - | chr7.1:85598782-85598801 | MS.gene74799:intron | 45.0% | |
| CTCGAATGACATACAGGGAA+AGG | - | chr7.1:85598579-85598598 | MS.gene74799:intron | 45.0% | |
| CTTTCCCTGTATGTCATTCG+AGG | + | chr7.1:85598581-85598600 | None:intergenic | 45.0% | |
| TAATGAACGTGGCATGCCTT+TGG | - | chr7.1:85598248-85598267 | MS.gene74799:intron | 45.0% | |
| TCGCATTATCACTGCCATTG+AGG | - | chr7.1:85597693-85597712 | MS.gene74799:CDS | 45.0% | |
| TGACATACAGGGAAAGGTGA+TGG | - | chr7.1:85598585-85598604 | MS.gene74799:intron | 45.0% | |
| TTCCCTGTATGTCATTCGAG+GGG | + | chr7.1:85598579-85598598 | None:intergenic | 45.0% | |
| ! | CATGCCTTTGGAGGAAAATC+TGG | - | chr7.1:85598260-85598279 | MS.gene74799:intron | 45.0% |
| ! | TCAATGGCAGTGATAATGCG+AGG | + | chr7.1:85597694-85597713 | None:intergenic | 45.0% |
| ACATGGTTGGAGAGATGGAG+AGG | - | chr7.1:85598787-85598806 | MS.gene74799:intron | 50.0% | |
| TCCCCCTCGAATGACATACA+GGG | - | chr7.1:85598574-85598593 | MS.gene74799:intron | 50.0% | |
| TCCCTGTATGTCATTCGAGG+GGG | + | chr7.1:85598578-85598597 | None:intergenic | 50.0% | |
| TTGTGTTCAGGTGTGAGCTG+AGG | - | chr7.1:85597888-85597907 | MS.gene74799:CDS | 50.0% | |
| CTCCCCCTCGAATGACATAC+AGG | - | chr7.1:85598573-85598592 | MS.gene74799:intron | 55.0% | |
| TGAACGTGGCATGCCTTTGG+AGG | - | chr7.1:85598251-85598270 | MS.gene74799:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 85597616 | 85598964 | 85597616 | ID=MS.gene74799 |
| chr7.1 | mRNA | 85597616 | 85598964 | 85597616 | ID=MS.gene74799.t1;Parent=MS.gene74799 |
| chr7.1 | exon | 85598849 | 85598964 | 85598849 | ID=MS.gene74799.t1.exon1;Parent=MS.gene74799.t1 |
| chr7.1 | CDS | 85598849 | 85598964 | 85598849 | ID=cds.MS.gene74799.t1;Parent=MS.gene74799.t1 |
| chr7.1 | exon | 85598606 | 85598682 | 85598606 | ID=MS.gene74799.t1.exon2;Parent=MS.gene74799.t1 |
| chr7.1 | CDS | 85598606 | 85598682 | 85598606 | ID=cds.MS.gene74799.t1;Parent=MS.gene74799.t1 |
| chr7.1 | exon | 85598481 | 85598560 | 85598481 | ID=MS.gene74799.t1.exon3;Parent=MS.gene74799.t1 |
| chr7.1 | CDS | 85598481 | 85598560 | 85598481 | ID=cds.MS.gene74799.t1;Parent=MS.gene74799.t1 |
| chr7.1 | exon | 85597878 | 85597985 | 85597878 | ID=MS.gene74799.t1.exon4;Parent=MS.gene74799.t1 |
| chr7.1 | CDS | 85597878 | 85597985 | 85597878 | ID=cds.MS.gene74799.t1;Parent=MS.gene74799.t1 |
| chr7.1 | exon | 85597616 | 85597801 | 85597616 | ID=MS.gene74799.t1.exon5;Parent=MS.gene74799.t1 |
| chr7.1 | CDS | 85597616 | 85597801 | 85597616 | ID=cds.MS.gene74799.t1;Parent=MS.gene74799.t1 |
| Gene Sequence |
| Protein sequence |
