AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75037

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75037.t1 MTR_6g090290 91.743 436 36 0 1 436 1 436 0.0 800
MS.gene75037.t1 MTR_8g096990 70.709 437 123 2 1 437 1 432 0.0 630
MS.gene75037.t1 MTR_5g080960 70.387 439 123 5 1 437 1 434 0.0 622
MS.gene75037.t1 MTR_5g080890 70.159 439 124 5 1 437 1 434 0.0 621
MS.gene75037.t1 MTR_8g038280 66.590 437 145 1 1 437 1 436 0.0 585
MS.gene75037.t1 MTR_5g044770 65.217 437 146 3 1 437 1 431 0.0 568
MS.gene75037.t1 MTR_8g038250 63.205 443 130 4 1 437 1 416 0.0 555
MS.gene75037.t1 MTR_4g109760 62.929 437 124 6 1 437 1 399 5.90e-176 498
MS.gene75037.t1 MTR_8g038310 58.352 437 157 5 1 436 1 413 1.12e-175 498
MS.gene75037.t1 MTR_3g028120 50.119 419 196 7 27 437 26 439 1.43e-138 404
MS.gene75037.t1 MTR_5g038360 48.284 437 209 10 9 436 8 436 4.36e-132 387
MS.gene75037.t1 MTR_6g088760 49.642 419 194 10 6 415 7 417 2.16e-129 384
MS.gene75037.t1 MTR_2g067830 49.757 412 194 9 32 436 1 406 3.65e-121 358
MS.gene75037.t1 MTR_0024s0040 48.571 420 193 10 30 436 1 410 5.96e-121 358
MS.gene75037.t1 MTR_5g044570 44.196 448 201 11 1 436 1 411 1.57e-116 347
MS.gene75037.t1 MTR_2g105300 47.433 409 180 11 32 433 1 381 3.05e-109 327
MS.gene75037.t1 MTR_0036s0070 39.597 447 238 12 1 436 1 426 9.89e-105 317
MS.gene75037.t1 MTR_0036s0050 40.674 445 233 13 9 436 5 435 2.42e-103 314
MS.gene75037.t1 MTR_0036s0110 37.555 458 248 12 1 436 1 442 4.88e-93 288
MS.gene75037.t1 MTR_5g008070 40.758 422 224 10 21 435 23 425 2.72e-91 283
MS.gene75037.t1 MTR_2g059490 41.779 371 202 9 75 437 47 411 7.05e-91 282
MS.gene75037.t1 MTR_5g008150 40.310 387 212 10 59 436 36 412 3.12e-90 280
MS.gene75037.t1 MTR_2g059460 40.000 420 202 14 26 436 28 406 1.45e-89 278
MS.gene75037.t1 MTR_5g008110 38.235 374 215 7 70 436 51 415 2.34e-86 270
MS.gene75037.t1 MTR_3g097150 33.925 451 262 11 6 437 16 449 5.27e-76 244
MS.gene75037.t1 MTR_1g060750 34.398 407 225 11 40 436 2 376 1.00e-65 215
MS.gene75037.t1 MTR_5g008090 37.377 305 172 9 141 436 20 314 4.91e-58 193
MS.gene75037.t1 MTR_4g045857 44.130 247 129 7 74 315 46 288 1.01e-57 192
MS.gene75037.t1 MTR_1g115210 33.333 429 256 11 21 434 73 486 2.32e-56 194
MS.gene75037.t1 MTR_1g039270 33.565 432 242 16 28 434 58 469 6.62e-56 192
MS.gene75037.t1 MTR_7g117430 33.028 436 253 13 19 434 68 484 3.77e-55 190
MS.gene75037.t1 MTR_3g046300 33.505 388 235 11 68 437 79 461 6.35e-46 165
MS.gene75037.t1 MTR_2g028190 31.169 462 276 17 4 434 1 451 1.24e-44 161
MS.gene75037.t1 MTR_1g111350 34.160 363 201 14 85 434 130 467 2.18e-44 161
MS.gene75037.t1 MTR_6g088765 39.095 243 109 5 162 403 5 209 3.17e-42 149
MS.gene75037.t1 MTR_6g085010 32.861 353 216 10 86 434 156 491 3.31e-42 155
MS.gene75037.t1 MTR_8g103880 30.851 376 237 12 66 434 96 455 5.54e-42 154
MS.gene75037.t1 MTR_5g091130 30.591 389 246 10 68 437 173 556 1.82e-40 152
MS.gene75037.t1 MTR_3g053710 31.319 364 223 10 85 434 116 466 1.10e-39 148
MS.gene75037.t1 MTR_4g007740 30.175 401 250 11 49 435 94 478 9.90e-39 146
MS.gene75037.t1 MTR_2g055840 28.177 362 220 12 87 422 14 361 8.09e-32 125
MS.gene75037.t1 MTR_3g053980 30.194 361 225 9 85 434 141 485 2.18e-29 120
MS.gene75037.t1 MTR_4g008110 53.333 120 29 1 158 277 45 137 1.08e-28 110
MS.gene75037.t1 MTR_2g070430 26.216 473 293 20 2 436 3 457 2.48e-27 114
MS.gene75037.t1 MTR_7g062360 27.068 399 243 16 72 437 45 428 2.14e-26 110
MS.gene75037.t1 MTR_3g068000 27.062 388 219 14 89 434 76 441 2.21e-25 108
MS.gene75037.t1 MTR_3g087780 26.134 463 275 19 17 437 16 453 2.38e-25 108
MS.gene75037.t1 MTR_4g095270 25.180 417 265 17 41 437 37 426 1.51e-24 106
MS.gene75037.t1 MTR_4g095270 25.180 417 265 17 41 437 37 426 1.72e-24 106
MS.gene75037.t1 MTR_1g078240 25.587 383 233 15 85 435 68 430 1.75e-24 105
MS.gene75037.t1 MTR_7g080230 23.937 447 273 16 30 435 11 431 1.96e-24 105
MS.gene75037.t1 MTR_2g063110 25.686 401 238 14 85 434 117 508 2.09e-24 105
MS.gene75037.t1 MTR_8g075090 28.042 378 215 16 87 436 113 461 6.68e-24 104
MS.gene75037.t1 MTR_8g094590 30.070 286 163 10 85 358 72 332 2.97e-23 101
MS.gene75037.t1 MTR_8g094590 31.439 264 150 9 85 342 72 310 3.88e-23 99.8
MS.gene75037.t1 MTR_8g094590 30.070 286 163 10 85 358 72 332 4.90e-23 101
MS.gene75037.t1 MTR_1g078240 25.521 384 233 16 85 435 68 431 5.85e-23 101
MS.gene75037.t1 MTR_3g065160 24.282 383 238 13 83 437 71 429 8.12e-23 100
MS.gene75037.t1 MTR_8g075090 27.704 379 211 17 87 434 113 459 1.10e-22 100
MS.gene75037.t1 MTR_5g016260 24.397 373 237 15 85 437 87 434 1.18e-22 100
MS.gene75037.t1 MTR_5g016260 24.397 373 237 15 85 437 87 434 1.33e-22 100
MS.gene75037.t1 MTR_5g061690 29.380 371 210 19 78 422 89 433 1.70e-22 100
MS.gene75037.t1 MTR_7g105850 26.273 491 269 24 9 436 7 467 1.83e-22 99.8
MS.gene75037.t1 MTR_2g084550 28.238 386 219 17 85 435 76 438 2.37e-22 99.8
MS.gene75037.t1 MTR_8g039540 28.509 456 283 19 1 435 1 434 1.14e-21 97.1
MS.gene75037.t1 MTR_0945s0010 38.583 127 73 3 314 437 1 125 1.33e-21 91.3
MS.gene75037.t1 MTR_7g021510 24.219 384 238 14 85 435 71 434 1.61e-21 97.1
MS.gene75037.t1 MTR_1g037630 26.291 426 275 17 26 435 27 429 1.72e-21 96.7
MS.gene75037.t1 MTR_4g056420 26.904 394 219 19 82 435 73 437 7.82e-21 95.1
MS.gene75037.t1 MTR_1g061940 24.842 475 274 23 8 435 7 445 2.13e-20 94.0
MS.gene75037.t1 MTR_3g014290 28.969 359 223 14 87 435 81 417 2.17e-20 93.2
MS.gene75037.t1 MTR_1g078240 26.814 317 188 13 85 377 68 364 2.66e-20 92.4
MS.gene75037.t1 MTR_7g102750 25.191 393 221 19 85 435 80 441 2.93e-20 93.2
MS.gene75037.t1 MTR_2g103340 40.000 125 30 2 21 145 34 113 3.43e-20 88.2
MS.gene75037.t1 MTR_3g087780 27.469 324 201 11 128 437 8 311 6.67e-20 91.3
MS.gene75037.t1 MTR_8g075090 28.116 345 193 15 92 409 118 434 7.60e-20 92.0
MS.gene75037.t1 MTR_3g087780 27.469 324 201 11 128 437 44 347 8.73e-20 91.3
MS.gene75037.t1 MTR_1g029810 25.521 384 228 19 85 437 56 412 1.67e-19 90.5
MS.gene75037.t1 MTR_6g084710 26.910 301 192 11 71 360 176 459 2.60e-19 90.5
MS.gene75037.t1 MTR_6g084710 25.000 392 249 14 71 434 176 550 2.60e-19 90.9
MS.gene75037.t1 MTR_1g029810 25.521 384 228 19 85 437 56 412 2.92e-19 90.1
MS.gene75037.t1 MTR_5g022880 25.876 371 230 13 87 434 113 461 1.03e-18 89.0
MS.gene75037.t1 MTR_3g096930 27.526 287 166 13 85 357 83 341 1.21e-18 88.2
MS.gene75037.t1 MTR_5g077850 23.057 386 243 13 83 437 78 440 2.14e-17 84.3
MS.gene75037.t1 MTR_7g102750 25.836 329 177 16 85 377 80 377 1.10e-16 82.0
MS.gene75037.t1 MTR_5g061690 29.293 297 173 15 138 422 23 294 1.23e-16 81.6
MS.gene75037.t1 MTR_4g100990 25.587 426 233 19 78 437 66 473 3.72e-16 80.9
MS.gene75037.t1 MTR_5g012490 26.008 496 270 25 6 435 6 470 5.76e-15 77.0
MS.gene75037.t1 MTR_4g023650 24.936 389 232 18 84 437 59 422 7.99e-15 76.3
MS.gene75037.t1 MTR_4g023630 23.969 388 237 15 84 437 59 422 9.27e-15 76.3
MS.gene75037.t1 MTR_5g077920 24.041 391 247 14 76 436 58 428 2.55e-14 75.1
MS.gene75037.t1 MTR_1g093100 24.742 388 239 17 67 419 17 386 1.51e-13 72.4
MS.gene75037.t1 MTR_2g037710 28.037 214 125 8 227 434 15 205 5.53e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75037.t1 AT1G64830 47.229 415 211 4 25 437 23 431 4.67e-131 385
MS.gene75037.t1 AT5G33340 46.860 414 211 5 27 437 30 437 5.58e-124 367
MS.gene75037.t1 AT2G35615 42.921 445 232 8 7 435 6 444 1.90e-110 333
MS.gene75037.t1 AT1G31450 41.685 451 238 10 1 435 1 442 2.36e-104 317
MS.gene75037.t1 AT2G28010 37.642 441 215 13 5 437 6 394 4.77e-82 258
MS.gene75037.t1 AT2G28030 41.176 357 177 9 87 437 61 390 6.53e-78 247
MS.gene75037.t1 AT2G28040 37.237 427 212 12 16 437 18 393 9.83e-77 244
MS.gene75037.t1 AT2G28040 37.740 416 203 12 27 437 20 384 1.15e-76 244
MS.gene75037.t1 AT2G28220 37.097 434 212 14 15 437 17 400 2.37e-74 238
MS.gene75037.t1 AT2G03200 35.714 434 244 11 25 437 42 461 3.55e-73 237
MS.gene75037.t1 AT2G28225 36.675 379 204 10 69 437 48 400 6.88e-69 224
MS.gene75037.t1 AT1G01300 37.396 361 201 12 85 435 140 485 6.63e-56 192
MS.gene75037.t1 AT2G23945 29.067 461 276 17 9 437 10 451 8.87e-53 183
MS.gene75037.t1 AT3G61820 34.704 389 232 10 55 434 108 483 4.07e-52 182
MS.gene75037.t1 AT4G30030 33.245 376 223 15 70 434 61 419 7.57e-50 175
MS.gene75037.t1 AT3G20015 30.023 443 259 15 20 434 51 470 8.35e-47 168
MS.gene75037.t1 AT3G18490 31.116 421 244 13 23 434 117 500 3.51e-45 164
MS.gene75037.t1 AT4G30040 29.955 444 262 17 9 429 10 427 1.74e-44 160
MS.gene75037.t1 AT1G25510 34.167 360 205 12 85 434 146 483 3.15e-44 161
MS.gene75037.t1 AT5G10770 32.964 361 216 9 85 435 130 474 3.00e-43 158
MS.gene75037.t1 AT5G10760 33.503 394 228 11 57 434 89 464 4.61e-43 157
MS.gene75037.t1 AT3G25700 28.637 433 252 16 39 435 39 450 2.10e-41 153
MS.gene75037.t1 AT2G42980 30.206 437 264 17 31 437 102 527 2.42e-39 148
MS.gene75037.t1 AT1G79720 32.249 369 210 15 86 434 134 482 1.44e-38 145
MS.gene75037.t1 AT5G36260 29.973 377 220 16 85 435 76 434 7.72e-36 138
MS.gene75037.t1 AT3G59080 28.827 392 242 13 72 437 155 535 8.71e-36 139
MS.gene75037.t1 AT3G12700 26.744 430 272 16 30 435 51 461 9.75e-30 120
MS.gene75037.t1 AT2G39710 29.288 379 210 16 89 429 67 425 1.37e-29 120
MS.gene75037.t1 AT1G65240 27.513 378 225 15 85 435 72 427 8.33e-29 118
MS.gene75037.t1 AT3G02740 29.820 389 221 19 74 435 77 440 1.11e-28 118
MS.gene75037.t1 AT4G12920 26.702 382 247 12 61 434 31 387 1.33e-28 116
MS.gene75037.t1 AT3G02740 29.521 376 218 18 87 435 10 365 2.35e-28 116
MS.gene75037.t1 AT3G54400 27.610 431 251 16 30 435 31 425 4.37e-27 112
MS.gene75037.t1 AT5G43100 24.495 396 258 15 63 437 45 420 5.99e-27 113
MS.gene75037.t1 AT3G59080 27.907 387 216 12 72 437 155 499 1.21e-26 112
MS.gene75037.t1 AT5G43100 24.495 396 258 15 63 437 45 420 1.42e-26 112
MS.gene75037.t1 AT3G50050 26.829 410 248 17 55 437 55 439 3.69e-26 111
MS.gene75037.t1 AT1G09750 28.649 370 221 15 85 434 102 448 2.14e-25 108
MS.gene75037.t1 AT5G45120 26.536 407 229 16 87 434 83 478 2.62e-25 108
MS.gene75037.t1 AT5G02190 28.150 373 222 15 96 434 82 442 5.86e-25 107
MS.gene75037.t1 AT1G66180 25.990 404 239 18 69 437 51 429 2.01e-24 105
MS.gene75037.t1 AT3G52500 30.348 402 208 23 85 435 88 468 1.25e-23 103
MS.gene75037.t1 AT5G37540 26.121 379 241 14 81 434 74 438 1.40e-23 102
MS.gene75037.t1 AT3G51350 27.212 452 260 20 27 436 30 454 7.96e-22 98.2
MS.gene75037.t1 AT5G22850 26.649 379 234 15 85 435 79 441 3.08e-21 96.3
MS.gene75037.t1 AT1G05840 24.409 381 239 12 85 435 78 439 2.34e-20 93.6
MS.gene75037.t1 AT1G44130 24.505 404 248 17 67 437 23 402 1.11e-19 90.9
MS.gene75037.t1 AT4G33490 23.907 389 228 18 85 437 58 414 1.19e-19 91.3
MS.gene75037.t1 AT2G17760 27.397 365 219 16 92 434 109 449 1.48e-19 91.3
MS.gene75037.t1 AT2G36670 24.868 378 240 15 85 435 98 458 3.55e-19 90.1
MS.gene75037.t1 AT2G36670 24.734 376 239 15 87 435 34 392 3.99e-19 89.7
MS.gene75037.t1 AT2G36670 24.734 376 239 15 87 435 34 392 3.99e-19 89.7
MS.gene75037.t1 AT2G36670 24.734 376 239 15 87 435 105 463 7.27e-19 89.4
MS.gene75037.t1 AT1G77480 24.282 383 235 18 85 437 65 422 7.76e-19 88.6
MS.gene75037.t1 AT1G77480 24.282 383 235 18 85 437 65 422 1.19e-18 88.6
MS.gene75037.t1 AT1G08210 24.672 381 236 15 85 435 130 489 3.91e-18 87.0
MS.gene75037.t1 AT5G07030 23.644 461 305 17 2 435 15 455 4.45e-18 86.7
MS.gene75037.t1 AT1G08210 24.672 381 236 15 85 435 116 475 5.22e-18 86.7
MS.gene75037.t1 AT1G08210 24.672 381 236 15 85 435 82 441 5.65e-18 86.7
MS.gene75037.t1 AT1G49050 21.253 367 237 13 104 436 49 397 9.78e-18 85.1
MS.gene75037.t1 AT5G10080 26.625 477 271 26 11 436 7 455 1.78e-17 85.1
MS.gene75037.t1 AT1G49050 21.594 389 251 14 84 436 200 570 1.89e-17 85.1
MS.gene75037.t1 AT4G33490 26.772 254 156 10 85 330 55 286 2.02e-17 84.3
MS.gene75037.t1 AT3G25700 29.121 182 108 6 39 207 39 212 3.00e-16 80.1
MS.gene75037.t1 AT1G08210 24.781 343 209 14 85 399 82 403 1.77e-14 75.9
MS.gene75037.t1 AT3G42550 24.074 378 213 13 77 435 68 390 2.57e-14 75.1
MS.gene75037.t1 AT1G49050 24.046 262 173 9 84 333 200 447 7.87e-14 73.6
MS.gene75037.t1 AT4G35880 23.118 372 229 17 93 437 113 454 2.25e-13 72.4

Find 66 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTCCTAAAACTGTTAT+AGG 0.142168 6.3:+83304033 MS.gene75037:CDS
GGAGATGCCGCAGTCGTTTC+TGG 0.174606 6.3:+83304240 MS.gene75037:CDS
TGTGAATATTCTATTAATTA+TGG 0.194092 6.3:+83303937 MS.gene75037:CDS
CTTAGCTCAACAAAATTTCT+TGG 0.236063 6.3:+83304686 MS.gene75037:CDS
GGGTCGAGTAAACTCAATTT+TGG 0.266363 6.3:+83304219 MS.gene75037:CDS
ACAATACCATATGACTTAAT+TGG 0.286512 6.3:-83303745 None:intergenic
GAATTCAGCAATGGTAGATT+TGG 0.327057 6.3:+83304455 MS.gene75037:CDS
TAGGAGATTACAGTTGATTC+AGG 0.336604 6.3:-83303682 None:intergenic
CACATCTCTAATAACACAAT+TGG 0.340665 6.3:+83304125 MS.gene75037:CDS
CATAGACTGAATTGTTGATT+AGG 0.346843 6.3:-83304501 None:intergenic
GATTAAACTTAGGAGGGGTT+TGG 0.366519 6.3:-83303825 None:intergenic
TTGTAACTTGATGACTTTGA+TGG 0.396449 6.3:-83303847 None:intergenic
AGCTCTGGTGTAGTTGGCCT+TGG 0.403335 6.3:+83304093 MS.gene75037:CDS
CCTAAAACTGTTATAGGATG+TGG 0.410701 6.3:+83304039 MS.gene75037:CDS
GACAAAAGAGTAGAATTTGC+AGG 0.417625 6.3:+83304345 MS.gene75037:CDS
CGCGTAAGCTCTGGTGTAGT+TGG 0.418544 6.3:+83304087 MS.gene75037:CDS
ACACAATTGGGACCGTCAAT+TGG 0.426973 6.3:+83304138 MS.gene75037:CDS
TCTAACGGTGGTGCCATCTT+CGG 0.431189 6.3:+83304660 MS.gene75037:CDS
TTAAAATGTGCGGTCACATA+AGG 0.431541 6.3:-83304558 None:intergenic
TGGTAAATTCTCTTATTGTT+TGG 0.432097 6.3:+83304161 MS.gene75037:CDS
GCTCAACAAAATTTCTTGGT+TGG 0.437753 6.3:+83304690 MS.gene75037:CDS
ACCCCACCAATTAAGTCATA+TGG 0.439961 6.3:+83303739 MS.gene75037:CDS
TTGAGCTAAGCTTCCGAAGA+TGG 0.446795 6.3:-83304673 None:intergenic
GAAGTGACCGACACGATTGA+TGG 0.448925 6.3:-83303635 None:intergenic
TCTGGTGTAGTTGGCCTTGG+AGG 0.451277 6.3:+83304096 MS.gene75037:CDS
TCGTTTAAACGCGTAAGCTC+TGG 0.455409 6.3:+83304078 MS.gene75037:CDS
ACAATAGAAGCATTTAGTGT+TGG 0.456653 6.3:+83304321 MS.gene75037:CDS
TTCAATTTAGTATAAACATC+AGG 0.457412 6.3:-83304435 None:intergenic
GGTGTAGTTGGCCTTGGAGG+TGG 0.457901 6.3:+83304099 MS.gene75037:CDS
GGATGTGGAACTAACAATAC+AGG 0.467964 6.3:+83304054 MS.gene75037:CDS
GACTTTGATGGATTAAACTT+AGG 0.476937 6.3:-83303835 None:intergenic
AAAAGAAATACTAAAACAAA+AGG 0.486755 6.3:-83303494 None:intergenic
ATACAGGTAGTGACATTGTA+TGG 0.494388 6.3:+83303770 MS.gene75037:CDS
TCATATGGTATTGTCGATAC+AGG 0.494460 6.3:+83303754 MS.gene75037:CDS
GAATTTGCAGGGTCTTCAAA+AGG 0.494554 6.3:+83304357 MS.gene75037:CDS
TTTGATGGATTAAACTTAGG+AGG 0.495954 6.3:-83303832 None:intergenic
ACATCTCTAATAACACAATT+GGG 0.500209 6.3:+83304126 MS.gene75037:CDS
GAGAATTTACCACCAATTGA+CGG 0.523865 6.3:-83304150 None:intergenic
TACCATATGACTTAATTGGT+GGG 0.543258 6.3:-83303741 None:intergenic
CAATTGTGTTATTAGAGATG+TGG 0.543750 6.3:-83304124 None:intergenic
AAATGAAATAGGACGGCCAT+TGG 0.543813 6.3:-83304016 None:intergenic
TCTTCAAAAGGTGTTGAAGA+AGG 0.544477 6.3:+83304369 MS.gene75037:CDS
TTGATGGATTAAACTTAGGA+GGG 0.554944 6.3:-83303831 None:intergenic
AATTGTGTTATTAGAGATGT+GGG 0.556322 6.3:-83304123 None:intergenic
TACTAAATTGAATTCAGCAA+TGG 0.567220 6.3:+83304446 MS.gene75037:CDS
ATACCATATGACTTAATTGG+TGG 0.567621 6.3:-83303742 None:intergenic
ATCTGCACCTTTAAAATGTG+CGG 0.576686 6.3:-83304568 None:intergenic
CAATTGGGACCGTCAATTGG+TGG 0.579391 6.3:+83304141 MS.gene75037:CDS
TCAACTGTAATCTCCTACGA+GGG 0.579524 6.3:+83303688 MS.gene75037:CDS
AAGATGGCACCACCGTTAGA+CGG 0.582649 6.3:-83304657 None:intergenic
TGATGGATTAAACTTAGGAG+GGG 0.584520 6.3:-83303830 None:intergenic
TGGTTGTAACATTGTTCACA+AGG 0.591014 6.3:-83303805 None:intergenic
GAGATGTGGGTCCACCTCCA+AGG 0.595636 6.3:-83304110 None:intergenic
ACCATATGACTTAATTGGTG+GGG 0.597911 6.3:-83303740 None:intergenic
TATATCATGAGCTATTCAGT+TGG 0.598495 6.3:+83303715 MS.gene75037:CDS
CTTCAACCCTCATTACACTT+CGG 0.599430 6.3:-83304760 None:intergenic
ACAAAAGAGTAGAATTTGCA+GGG 0.643770 6.3:+83304346 MS.gene75037:CDS
CATGATATAATCACCCTCGT+AGG 0.657331 6.3:-83303701 None:intergenic
AATTTAGTATAAACATCAGG+TGG 0.660177 6.3:-83304432 None:intergenic
GGCAATCAATCACATTCACA+AGG 0.662936 6.3:+83303958 MS.gene75037:CDS
CTTACATTAGAATCTACCAA+TGG 0.676774 6.3:+83304000 MS.gene75037:CDS
CGTCGTTCCATCAATCGTGT+CGG 0.677525 6.3:+83303628 MS.gene75037:CDS
GATGTGGAACTAACAATACA+GGG 0.681631 6.3:+83304055 MS.gene75037:CDS
GTACTGCATTGACAACATGT+TGG 0.687626 6.3:-83303606 None:intergenic
ATCAACTGTAATCTCCTACG+AGG 0.740288 6.3:+83303687 MS.gene75037:CDS
ATTGTGACCAGAAACGACTG+CGG 0.749221 6.3:-83304247 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAAAAAATATGTCAAT+GGG + chr6.3:83304198-83304217 MS.gene75037:CDS 10.0%
!! TATATTAAAAAATATGTCAA+TGG + chr6.3:83304197-83304216 MS.gene75037:CDS 10.0%
!! AAAAGAAATACTAAAACAAA+AGG - chr6.3:83303497-83303516 None:intergenic 15.0%
!! TATTAAAAAATATGTCAATG+GGG + chr6.3:83304199-83304218 MS.gene75037:CDS 15.0%
!! TCAAATAGTATAAAAATGAA+GGG - chr6.3:83304302-83304321 None:intergenic 15.0%
!! TGTGAATATTCTATTAATTA+TGG + chr6.3:83303937-83303956 MS.gene75037:CDS 15.0%
!! AACAAAAGGAAAAGAAAAAA+AGG - chr6.3:83303483-83303502 None:intergenic 20.0%
!! ACAAAAGGAAAAGAAAAAAA+GGG - chr6.3:83303482-83303501 None:intergenic 20.0%
!! GTCAAATAGTATAAAAATGA+AGG - chr6.3:83304303-83304322 None:intergenic 20.0%
!! TTCAATTTAGTATAAACATC+AGG - chr6.3:83304438-83304457 None:intergenic 20.0%
! AAAGAAAAAAAGGGTAAGAA+AGG - chr6.3:83303473-83303492 None:intergenic 25.0%
! AATTTAGTATAAACATCAGG+TGG - chr6.3:83304435-83304454 None:intergenic 25.0%
! ACAATACCATATGACTTAAT+TGG - chr6.3:83303748-83303767 None:intergenic 25.0%
! ACATCTCTAATAACACAATT+GGG + chr6.3:83304126-83304145 MS.gene75037:CDS 25.0%
! TACTAAATTGAATTCAGCAA+TGG + chr6.3:83304446-83304465 MS.gene75037:CDS 25.0%
! TCATTTCCTAAAACTGTTAT+AGG + chr6.3:83304033-83304052 MS.gene75037:CDS 25.0%
! TGGTAAATTCTCTTATTGTT+TGG + chr6.3:83304161-83304180 MS.gene75037:CDS 25.0%
!! AATTGTGTTATTAGAGATGT+GGG - chr6.3:83304126-83304145 None:intergenic 25.0%
!!! ACAGTTTTAGGAAATGAAAT+AGG - chr6.3:83304030-83304049 None:intergenic 25.0%
!!! TGTTGGTATTTATTTTGTGT+AGG - chr6.3:83303592-83303611 None:intergenic 25.0%
!!! TTTCTTTTTCTCAAGTTGTA+AGG + chr6.3:83303509-83303528 MS.gene75037:CDS 25.0%
!!! TTTTAGGAAATGAAATAGGA+CGG - chr6.3:83304026-83304045 None:intergenic 25.0%
ACAAAAGAGTAGAATTTGCA+GGG + chr6.3:83304346-83304365 MS.gene75037:CDS 30.0%
ATACCATATGACTTAATTGG+TGG - chr6.3:83303745-83303764 None:intergenic 30.0%
CACATCTCTAATAACACAAT+TGG + chr6.3:83304125-83304144 MS.gene75037:CDS 30.0%
CTTACATTAGAATCTACCAA+TGG + chr6.3:83304000-83304019 MS.gene75037:CDS 30.0%
CTTAGCTCAACAAAATTTCT+TGG + chr6.3:83304686-83304705 MS.gene75037:CDS 30.0%
TACCATATGACTTAATTGGT+GGG - chr6.3:83303744-83303763 None:intergenic 30.0%
TATATCATGAGCTATTCAGT+TGG + chr6.3:83303715-83303734 MS.gene75037:CDS 30.0%
! CAATTGTGTTATTAGAGATG+TGG - chr6.3:83304127-83304146 None:intergenic 30.0%
! CATAGACTGAATTGTTGATT+AGG - chr6.3:83304504-83304523 None:intergenic 30.0%
! TTGTAACTTGATGACTTTGA+TGG - chr6.3:83303850-83303869 None:intergenic 30.0%
! TTTTGTGTAGGCTTGTAAAA+TGG - chr6.3:83303580-83303599 None:intergenic 30.0%
!! ACAATAGAAGCATTTAGTGT+TGG + chr6.3:83304321-83304340 MS.gene75037:CDS 30.0%
!! GACTTTGATGGATTAAACTT+AGG - chr6.3:83303838-83303857 None:intergenic 30.0%
!! TTGATGGATTAAACTTAGGA+GGG - chr6.3:83303834-83303853 None:intergenic 30.0%
!! TTTGATGGATTAAACTTAGG+AGG - chr6.3:83303835-83303854 None:intergenic 30.0%
!! TTTTCTCAAGTTGTAAGGAA+TGG + chr6.3:83303514-83303533 MS.gene75037:CDS 30.0%
!!! AAGACACAGTTTTTTGTTGA+AGG - chr6.3:83304722-83304741 None:intergenic 30.0%
!!! GAAGGGTCTTTTTTAACTAT+AGG - chr6.3:83304285-83304304 None:intergenic 30.0%
ACCATATGACTTAATTGGTG+GGG - chr6.3:83303743-83303762 None:intergenic 35.0%
ATACAGGTAGTGACATTGTA+TGG + chr6.3:83303770-83303789 MS.gene75037:CDS 35.0%
CCTAAAACTGTTATAGGATG+TGG + chr6.3:83304039-83304058 MS.gene75037:CDS 35.0%
GAATTCAGCAATGGTAGATT+TGG + chr6.3:83304455-83304474 MS.gene75037:CDS 35.0%
GACAAAAGAGTAGAATTTGC+AGG + chr6.3:83304345-83304364 MS.gene75037:CDS 35.0%
GAGAATTTACCACCAATTGA+CGG - chr6.3:83304153-83304172 None:intergenic 35.0%
GATGTGGAACTAACAATACA+GGG + chr6.3:83304055-83304074 MS.gene75037:CDS 35.0%
GCTCAACAAAATTTCTTGGT+TGG + chr6.3:83304690-83304709 MS.gene75037:CDS 35.0%
TCATATGGTATTGTCGATAC+AGG + chr6.3:83303754-83303773 MS.gene75037:CDS 35.0%
TGGTTGTAACATTGTTCACA+AGG - chr6.3:83303808-83303827 None:intergenic 35.0%
TTAAAATGTGCGGTCACATA+AGG - chr6.3:83304561-83304580 None:intergenic 35.0%
! CCACATCCTATAACAGTTTT+AGG - chr6.3:83304042-83304061 None:intergenic 35.0%
! TAGGAGATTACAGTTGATTC+AGG - chr6.3:83303685-83303704 None:intergenic 35.0%
! TATGTGACCGCACATTTTAA+AGG + chr6.3:83304561-83304580 MS.gene75037:CDS 35.0%
!! ATCTGCACCTTTAAAATGTG+CGG - chr6.3:83304571-83304590 None:intergenic 35.0%
!! ATGGTGATTTTAACGAGTCA+CGG - chr6.3:83303561-83303580 None:intergenic 35.0%
!! TCTTCAAAAGGTGTTGAAGA+AGG + chr6.3:83304369-83304388 MS.gene75037:CDS 35.0%
!! TGATGGATTAAACTTAGGAG+GGG - chr6.3:83303833-83303852 None:intergenic 35.0%
AAATGAAATAGGACGGCCAT+TGG - chr6.3:83304019-83304038 None:intergenic 40.0%
ACCCCACCAATTAAGTCATA+TGG + chr6.3:83303739-83303758 MS.gene75037:CDS 40.0%
ATCAACTGTAATCTCCTACG+AGG + chr6.3:83303687-83303706 MS.gene75037:CDS 40.0%
CATGATATAATCACCCTCGT+AGG - chr6.3:83303704-83303723 None:intergenic 40.0%
GAATTTGCAGGGTCTTCAAA+AGG + chr6.3:83304357-83304376 MS.gene75037:CDS 40.0%
GATTAAACTTAGGAGGGGTT+TGG - chr6.3:83303828-83303847 None:intergenic 40.0%
GGATGTGGAACTAACAATAC+AGG + chr6.3:83304054-83304073 MS.gene75037:CDS 40.0%
GGCAATCAATCACATTCACA+AGG + chr6.3:83303958-83303977 MS.gene75037:CDS 40.0%
TCAACTGTAATCTCCTACGA+GGG + chr6.3:83303688-83303707 MS.gene75037:CDS 40.0%
! GGGTCGAGTAAACTCAATTT+TGG + chr6.3:83304219-83304238 MS.gene75037:CDS 40.0%
! GTACTGCATTGACAACATGT+TGG - chr6.3:83303609-83303628 None:intergenic 40.0%
!! TTTGCTTTTGCTCCGTCTAA+CGG + chr6.3:83304645-83304664 MS.gene75037:CDS 40.0%
ACACAATTGGGACCGTCAAT+TGG + chr6.3:83304138-83304157 MS.gene75037:CDS 45.0%
ATTGTGACCAGAAACGACTG+CGG - chr6.3:83304250-83304269 None:intergenic 45.0%
TCGTTTAAACGCGTAAGCTC+TGG + chr6.3:83304078-83304097 MS.gene75037:CDS 45.0%
TTGAGCTAAGCTTCCGAAGA+TGG - chr6.3:83304676-83304695 None:intergenic 45.0%
AAGATGGCACCACCGTTAGA+CGG - chr6.3:83304660-83304679 None:intergenic 50.0%
CAATTGGGACCGTCAATTGG+TGG + chr6.3:83304141-83304160 MS.gene75037:CDS 50.0%
CGTCGTTCCATCAATCGTGT+CGG + chr6.3:83303628-83303647 MS.gene75037:CDS 50.0%
GAAGTGACCGACACGATTGA+TGG - chr6.3:83303638-83303657 None:intergenic 50.0%
!! TCTAACGGTGGTGCCATCTT+CGG + chr6.3:83304660-83304679 MS.gene75037:CDS 50.0%
! AGCTCTGGTGTAGTTGGCCT+TGG + chr6.3:83304093-83304112 MS.gene75037:CDS 55.0%
! CGCGTAAGCTCTGGTGTAGT+TGG + chr6.3:83304087-83304106 MS.gene75037:CDS 55.0%
! GCTTTTGCTCCGTCTAACGG+TGG + chr6.3:83304648-83304667 MS.gene75037:CDS 55.0%
!! TCTGGTGTAGTTGGCCTTGG+AGG + chr6.3:83304096-83304115 MS.gene75037:CDS 55.0%
GAGATGTGGGTCCACCTCCA+AGG - chr6.3:83304113-83304132 None:intergenic 60.0%
GGAGATGCCGCAGTCGTTTC+TGG + chr6.3:83304240-83304259 MS.gene75037:CDS 60.0%
!! GGTGTAGTTGGCCTTGGAGG+TGG + chr6.3:83304099-83304118 MS.gene75037:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 83303457 83304770 83303457 ID=MS.gene75037
chr6.3 mRNA 83303457 83304770 83303457 ID=MS.gene75037.t1;Parent=MS.gene75037
chr6.3 exon 83303457 83304770 83303457 ID=MS.gene75037.t1.exon1;Parent=MS.gene75037.t1
chr6.3 CDS 83303457 83304770 83303457 ID=cds.MS.gene75037.t1;Parent=MS.gene75037.t1
Gene Sequence

>MS.gene75037

ATGAACACACTTTCCTTTCTTACCCTTTTTTTCTTTTCCTTTTGTTTTAGTATTTCTTTTTCTCAAGTTGTAAGGAATGGTTTTAGTGTTGAACTTATACACCGTGACTCGTTAAAATCACCATTTTACAAGCCTACACAAAATAAATACCAACATGTTGTCAATGCAGTACGTCGTTCCATCAATCGTGTCGGTCACTTCTACAAGAATTCTCTCGCAAGCACACCTGAATCAACTGTAATCTCCTACGAGGGTGATTATATCATGAGCTATTCAGTTGGTACCCCACCAATTAAGTCATATGGTATTGTCGATACAGGTAGTGACATTGTATGGCTACAATGCGAGCCTTGTGAACAATGTTACAACCAAACCCCTCCTAAGTTTAATCCATCAAAGTCATCAAGTTACAAAAACATTTCTTGTTCGTCTAAACTATGTCAATCTGTGAAAGATATCTCTTGCAATGACAAAAAAAATTGTGAATATTCTATTAATTATGGCAATCAATCACATTCACAAGGAGATCTTAGTGTTGAGACTCTTACATTAGAATCTACCAATGGCCGTCCTATTTCATTTCCTAAAACTGTTATAGGATGTGGAACTAACAATACAGGGTCGTTTAAACGCGTAAGCTCTGGTGTAGTTGGCCTTGGAGGTGGACCCACATCTCTAATAACACAATTGGGACCGTCAATTGGTGGTAAATTCTCTTATTGTTTGGTGCGAATGTCAATTATATTAAAAAATATGTCAATGGGGTCGAGTAAACTCAATTTTGGAGATGCCGCAGTCGTTTCTGGTCACAATGTATTGTCAACTCCTATAGTTAAAAAAGACCCTTCATTTTTATACTATTTGACAATAGAAGCATTTAGTGTTGGAGACAAAAGAGTAGAATTTGCAGGGTCTTCAAAAGGTGTTGAAGAAGGTAACATCATAATTGATTCAAGTACAATTGTGACGTTTGTACCACCTGATGTTTATACTAAATTGAATTCAGCAATGGTAGATTTGGTGACACTAGAACGTGTTGATGATCCTAATCAACAATTCAGTCTATGTTATAATTCGTCGTCAGATGAAGAATATGATTTTCCTTATGTGACCGCACATTTTAAAGGTGCAGATATTTTGCTGTATGCTACTAATACGTTTATCGAAGTTGCTAGAGATGTTCTTTGCTTTGCTTTTGCTCCGTCTAACGGTGGTGCCATCTTCGGAAGCTTAGCTCAACAAAATTTCTTGGTTGGTTATGACCTTCAACAAAAAACTGTGTCTTTTAAGTCTATAGATTGTACCGAAGTGTAA
Protein sequence

>MS.gene75037.t1

MNTLSFLTLFFFSFCFSISFSQVVRNGFSVELIHRDSLKSPFYKPTQNKYQHVVNAVRRSINRVGHFYKNSLASTPESTVISYEGDYIMSYSVGTPPIKSYGIVDTGSDIVWLQCEPCEQCYNQTPPKFNPSKSSSYKNISCSSKLCQSVKDISCNDKKNCEYSINYGNQSHSQGDLSVETLTLESTNGRPISFPKTVIGCGTNNTGSFKRVSSGVVGLGGGPTSLITQLGPSIGGKFSYCLVRMSIILKNMSMGSSKLNFGDAAVVSGHNVLSTPIVKKDPSFLYYLTIEAFSVGDKRVEFAGSSKGVEEGNIIIDSSTIVTFVPPDVYTKLNSAMVDLVTLERVDDPNQQFSLCYNSSSDEEYDFPYVTAHFKGADILLYATNTFIEVARDVLCFAFAPSNGGAIFGSLAQQNFLVGYDLQQKTVSFKSIDCTEV