Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75127.t1 | XP_024641792.1 | 94.1 | 762 | 20 | 2 | 1 | 737 | 1 | 762 | 0.00E+00 | 1527.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75127.t1 | Q764T8 | 83.4 | 758 | 95 | 4 | 1 | 730 | 1 | 755 | 0.0e+00 | 1343.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75127.t1 | A0A072UN62 | 94.1 | 762 | 20 | 2 | 1 | 737 | 1 | 762 | 0.0e+00 | 1527.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058042 | MS.gene75127 | 0.807762 | 4.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75127.t1 | MTR_6g093180 | 94.094 | 762 | 20 | 2 | 1 | 737 | 1 | 762 | 0.0 | 1492 |
| MS.gene75127.t1 | MTR_6g034985 | 78.478 | 762 | 138 | 3 | 1 | 736 | 1 | 762 | 0.0 | 1267 |
| MS.gene75127.t1 | MTR_6g035010 | 75.399 | 752 | 160 | 2 | 1 | 727 | 1 | 752 | 0.0 | 1188 |
| MS.gene75127.t1 | MTR_4g005190 | 59.205 | 755 | 280 | 4 | 1 | 727 | 1 | 755 | 0.0 | 955 |
| MS.gene75127.t1 | MTR_4g005270 | 58.278 | 755 | 287 | 4 | 1 | 727 | 1 | 755 | 0.0 | 946 |
| MS.gene75127.t1 | MTR_0013s0230 | 57.672 | 756 | 291 | 5 | 1 | 727 | 1 | 756 | 0.0 | 937 |
| MS.gene75127.t1 | MTR_8g016975 | 56.053 | 760 | 306 | 4 | 1 | 732 | 1 | 760 | 0.0 | 928 |
| MS.gene75127.t1 | MTR_5g008810 | 58.940 | 755 | 282 | 5 | 1 | 728 | 1 | 754 | 0.0 | 922 |
| MS.gene75127.t1 | MTR_6g088140 | 55.526 | 760 | 286 | 8 | 1 | 728 | 1 | 740 | 0.0 | 882 |
| MS.gene75127.t1 | MTR_6g093100 | 55.097 | 775 | 300 | 10 | 1 | 728 | 1 | 774 | 0.0 | 880 |
| MS.gene75127.t1 | MTR_8g016960 | 53.236 | 757 | 319 | 6 | 1 | 728 | 1 | 751 | 0.0 | 879 |
| MS.gene75127.t1 | MTR_7g062020 | 54.368 | 767 | 310 | 7 | 1 | 728 | 1 | 766 | 0.0 | 877 |
| MS.gene75127.t1 | MTR_8g017020 | 52.500 | 760 | 299 | 6 | 1 | 732 | 1 | 726 | 0.0 | 855 |
| MS.gene75127.t1 | MTR_7g034535 | 57.742 | 691 | 265 | 4 | 64 | 727 | 1 | 691 | 0.0 | 853 |
| MS.gene75127.t1 | MTR_4g005190 | 58.445 | 669 | 250 | 4 | 1 | 641 | 1 | 669 | 0.0 | 830 |
| MS.gene75127.t1 | MTR_3g436160 | 58.471 | 667 | 247 | 4 | 87 | 727 | 32 | 694 | 0.0 | 820 |
| MS.gene75127.t1 | MTR_6g088140 | 54.167 | 672 | 257 | 7 | 1 | 641 | 1 | 652 | 0.0 | 757 |
| MS.gene75127.t1 | MTR_8g016940 | 55.556 | 270 | 118 | 1 | 463 | 732 | 2 | 269 | 2.68e-102 | 315 |
| MS.gene75127.t1 | MTR_8g016570 | 61.290 | 62 | 24 | 0 | 667 | 728 | 28 | 89 | 7.64e-20 | 86.7 |
| MS.gene75127.t1 | MTR_8g017040 | 57.143 | 70 | 28 | 1 | 1 | 68 | 1 | 70 | 5.28e-19 | 84.0 |
| MS.gene75127.t1 | MTR_3g436170 | 45.679 | 81 | 42 | 1 | 1 | 79 | 1 | 81 | 2.92e-17 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75127.t1 | AT2G07050 | 59.367 | 758 | 280 | 5 | 1 | 731 | 1 | 757 | 0.0 | 964 |
| MS.gene75127.t1 | AT1G78960 | 56.316 | 760 | 303 | 5 | 1 | 731 | 1 | 760 | 0.0 | 910 |
| MS.gene75127.t1 | AT1G78960 | 56.316 | 760 | 303 | 5 | 1 | 731 | 1 | 760 | 0.0 | 910 |
| MS.gene75127.t1 | AT1G78960 | 56.316 | 760 | 303 | 5 | 1 | 731 | 1 | 760 | 0.0 | 910 |
| MS.gene75127.t1 | AT1G78955 | 57.407 | 756 | 293 | 4 | 1 | 727 | 1 | 756 | 0.0 | 909 |
| MS.gene75127.t1 | AT1G78950 | 56.217 | 756 | 302 | 4 | 1 | 727 | 10 | 765 | 0.0 | 897 |
| MS.gene75127.t1 | AT1G78950 | 56.217 | 756 | 302 | 4 | 1 | 727 | 1 | 756 | 0.0 | 896 |
| MS.gene75127.t1 | AT2G07050 | 60.241 | 664 | 237 | 4 | 94 | 731 | 4 | 666 | 0.0 | 863 |
| MS.gene75127.t1 | AT1G66960 | 54.183 | 753 | 316 | 4 | 1 | 724 | 1 | 753 | 0.0 | 863 |
| MS.gene75127.t1 | AT1G66960 | 54.183 | 753 | 316 | 4 | 1 | 724 | 1 | 753 | 0.0 | 863 |
| MS.gene75127.t1 | AT1G66960 | 54.183 | 753 | 316 | 4 | 1 | 724 | 10 | 762 | 0.0 | 863 |
| MS.gene75127.t1 | AT1G66960 | 54.183 | 753 | 316 | 4 | 1 | 724 | 1 | 753 | 0.0 | 863 |
| MS.gene75127.t1 | AT1G78960 | 56.293 | 723 | 287 | 5 | 1 | 694 | 1 | 723 | 0.0 | 862 |
| MS.gene75127.t1 | AT1G78970 | 54.954 | 757 | 309 | 6 | 1 | 728 | 1 | 754 | 0.0 | 859 |
| MS.gene75127.t1 | AT1G78970 | 54.954 | 757 | 309 | 6 | 1 | 728 | 1 | 754 | 0.0 | 859 |
| MS.gene75127.t1 | AT1G78970 | 54.954 | 757 | 309 | 6 | 1 | 728 | 1 | 754 | 0.0 | 859 |
| MS.gene75127.t1 | AT1G78955 | 57.391 | 690 | 268 | 3 | 64 | 727 | 2 | 691 | 0.0 | 835 |
| MS.gene75127.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene75127.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene75127.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene75127.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene75127.t1 | AT3G45130 | 56.596 | 758 | 297 | 7 | 1 | 729 | 1 | 755 | 0.0 | 822 |
| MS.gene75127.t1 | AT1G78955 | 56.621 | 657 | 259 | 3 | 64 | 694 | 2 | 658 | 0.0 | 779 |
| MS.gene75127.t1 | AT1G78500 | 49.736 | 758 | 346 | 7 | 1 | 723 | 1 | 758 | 0.0 | 764 |
| MS.gene75127.t1 | AT1G78500 | 49.736 | 758 | 346 | 7 | 1 | 723 | 1 | 758 | 0.0 | 764 |
| MS.gene75127.t1 | AT1G78960 | 57.635 | 609 | 232 | 3 | 149 | 731 | 1 | 609 | 0.0 | 762 |
| MS.gene75127.t1 | AT5G36150 | 47.884 | 756 | 365 | 5 | 1 | 727 | 1 | 756 | 0.0 | 761 |
| MS.gene75127.t1 | AT5G36150 | 47.884 | 756 | 365 | 5 | 1 | 727 | 1 | 756 | 0.0 | 761 |
| MS.gene75127.t1 | AT5G48010 | 47.375 | 762 | 361 | 9 | 1 | 725 | 1 | 759 | 0.0 | 738 |
| MS.gene75127.t1 | AT4G15370 | 47.354 | 756 | 358 | 7 | 1 | 723 | 1 | 749 | 0.0 | 737 |
| MS.gene75127.t1 | AT4G15370 | 47.167 | 759 | 358 | 7 | 1 | 723 | 1 | 752 | 0.0 | 735 |
| MS.gene75127.t1 | AT4G15340 | 47.320 | 765 | 369 | 7 | 1 | 731 | 1 | 765 | 0.0 | 734 |
| MS.gene75127.t1 | AT5G42600 | 47.012 | 753 | 368 | 7 | 1 | 723 | 1 | 752 | 0.0 | 724 |
| MS.gene75127.t1 | AT5G48010 | 46.588 | 762 | 359 | 10 | 1 | 725 | 1 | 751 | 0.0 | 716 |
| MS.gene75127.t1 | AT1G78955 | 58.520 | 581 | 215 | 3 | 173 | 727 | 1 | 581 | 0.0 | 711 |
| MS.gene75127.t1 | AT1G78955 | 56.219 | 603 | 238 | 3 | 64 | 640 | 2 | 604 | 0.0 | 709 |
| MS.gene75127.t1 | AT3G29255 | 43.617 | 752 | 335 | 9 | 2 | 723 | 6 | 698 | 0.0 | 636 |
| MS.gene75127.t1 | AT4G15340 | 46.127 | 581 | 279 | 7 | 1 | 547 | 1 | 581 | 0.0 | 533 |
| MS.gene75127.t1 | AT1G78480 | 34.615 | 156 | 87 | 5 | 49 | 197 | 29 | 176 | 1.82e-18 | 84.7 |
Find 211 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTGCACGGTTGTCTTCT+AGG | 0.195387 | 6.3:+84561650 | None:intergenic |
| TGGGCTTTGTTGTCCCTTAT+CGG | 0.261922 | 6.3:-84561613 | MS.gene75127:CDS |
| ATCCACTTCCACCAGAATTC+TGG | 0.268631 | 6.3:-84564054 | MS.gene75127:CDS |
| ATGCCCATGTCATATCTATA+TGG | 0.269795 | 6.3:-84563871 | MS.gene75127:CDS |
| TTCCATCATGGGGAAAGTTC+TGG | 0.280761 | 6.3:-84564207 | MS.gene75127:CDS |
| CCCGAATCCCTGATTACTTC+TGG | 0.281207 | 6.3:-84563358 | MS.gene75127:CDS |
| GCCTTGTCTTCACCATCTTC+AGG | 0.293538 | 6.3:+84564280 | None:intergenic |
| CTTCTCTCAGTGATTTGATT+AGG | 0.298981 | 6.3:+84563821 | None:intergenic |
| GGATGAATAGGGGTGAATTT+TGG | 0.300808 | 6.3:+84564025 | None:intergenic |
| AGTTTAACTGCTGGTGGAAT+GGG | 0.308591 | 6.3:+84566204 | None:intergenic |
| TTTGGTAGAAACCAGAATTC+TGG | 0.308766 | 6.3:+84564043 | None:intergenic |
| CAATGGAGAGATGCAGAAAT+TGG | 0.310238 | 6.3:-84564258 | MS.gene75127:CDS |
| ATAATATATATACCTTGTTT+TGG | 0.312394 | 6.3:+84561743 | None:intergenic |
| AGAGAAGAAGATATCATAAC+AGG | 0.329769 | 6.3:-84566180 | MS.gene75127:CDS |
| TTGTGCTATGACCCTCTATA+TGG | 0.332950 | 6.3:+84564353 | None:intergenic |
| TAACTTCATTCCACGGTGTA+TGG | 0.336068 | 6.3:+84561455 | None:intergenic |
| TCTTGTGGTGTTCCTGCATT+TGG | 0.337805 | 6.3:+84571248 | None:intergenic |
| AGGTATGCACCTGGATGAAT+AGG | 0.345041 | 6.3:+84564013 | None:intergenic |
| GTAATGGCCCTCTATGTTAC+AGG | 0.353508 | 6.3:-84565835 | MS.gene75127:CDS |
| TTCGATATGATGAGTAGTTC+AGG | 0.355276 | 6.3:+84561258 | None:intergenic |
| GAGGTCAGAACTTTGTTTGA+TGG | 0.356213 | 6.3:+84571175 | None:intergenic |
| CATAACATATCTTGAATCAT+AGG | 0.357423 | 6.3:+84563590 | None:intergenic |
| TTCAATGAAATGAATTGCTT+TGG | 0.360615 | 6.3:+84562098 | None:intergenic |
| ACAATTCAAGGTGCAGCATT+TGG | 0.361763 | 6.3:+84561176 | None:intergenic |
| ACTCCACAGGTACGGCTGTT+GGG | 0.364012 | 6.3:-84561920 | MS.gene75127:intron |
| CTATGATTCAAGATATGTTA+TGG | 0.368024 | 6.3:-84563589 | MS.gene75127:CDS |
| AGTTTGTATGCCTCTGAGTT+CGG | 0.368801 | 6.3:+84563389 | None:intergenic |
| AAATTCTTACAGTTGGTTAG+AGG | 0.371127 | 6.3:-84562478 | MS.gene75127:intron |
| GTTTGTATGCCTCTGAGTTC+GGG | 0.373213 | 6.3:+84563390 | None:intergenic |
| CCTTGACCATGGTGGTTTAG+TGG | 0.374435 | 6.3:-84564233 | MS.gene75127:CDS |
| TTCCAGTTGATTTGATCATA+AGG | 0.375182 | 6.3:+84563784 | None:intergenic |
| AACCTTAGCAATAGTATTCC+GGG | 0.376806 | 6.3:+84563756 | None:intergenic |
| GGATCCCTTGATGAGGTTCT+AGG | 0.377958 | 6.3:-84565814 | MS.gene75127:CDS |
| GCCAGAAGTAATCAGGGATT+CGG | 0.379265 | 6.3:+84563357 | None:intergenic |
| TCTGCAAGCCAGAAGTAATC+AGG | 0.379292 | 6.3:+84563350 | None:intergenic |
| CAGTTTAACTGCTGGTGGAA+TGG | 0.379805 | 6.3:+84566203 | None:intergenic |
| TTGTCAAAGCAGCTTCCTAA+TGG | 0.380943 | 6.3:-84561793 | MS.gene75127:CDS |
| ATTTCTGTGAATAACTTCAT+AGG | 0.384332 | 6.3:-84571293 | MS.gene75127:CDS |
| ATCGAAACATCTTTCCCATA+TGG | 0.386037 | 6.3:-84561241 | MS.gene75127:CDS |
| TTGTTGTCCCTTATCGGTGC+AGG | 0.386134 | 6.3:-84561607 | MS.gene75127:CDS |
| TGTCAAAGCAGCTTCCTAAT+GGG | 0.387123 | 6.3:-84561792 | MS.gene75127:CDS |
| AATAATGAATGCAGAGCTTT+GGG | 0.387482 | 6.3:-84562971 | MS.gene75127:intron |
| AACCTTATGATCAAATCAAC+TGG | 0.400633 | 6.3:-84563786 | MS.gene75127:CDS |
| TTTCCTTAGCTCTATGAGTT+GGG | 0.400737 | 6.3:+84562136 | None:intergenic |
| TAATAATGAATGCAGAGCTT+TGG | 0.407402 | 6.3:-84562972 | MS.gene75127:intron |
| TAATGGCGGGTTCCCTGCTT+GGG | 0.410324 | 6.3:-84562508 | MS.gene75127:CDS |
| TATGATTCAAGATATGTTAT+GGG | 0.411393 | 6.3:-84563588 | MS.gene75127:CDS |
| ATACTCTCCCAGAGCCCATA+TGG | 0.417018 | 6.3:+84561226 | None:intergenic |
| GGTATGCACCTGGATGAATA+GGG | 0.417291 | 6.3:+84564014 | None:intergenic |
| GAGGGTCATAGCACAATGTT+TGG | 0.419263 | 6.3:-84564346 | MS.gene75127:CDS |
| CATCTTTCCCATATGGGCTC+TGG | 0.420694 | 6.3:-84561234 | MS.gene75127:CDS |
| GTAGCTTTGTGAAGAGATAC+AGG | 0.421470 | 6.3:+84562160 | None:intergenic |
| GATTTGATTAGGTCAGTGAT+AGG | 0.421520 | 6.3:+84563832 | None:intergenic |
| CACTGGCCTGCAGAATCTGC+TGG | 0.422487 | 6.3:-84566090 | MS.gene75127:CDS |
| TCTTCTCTCAGTTTAACTGC+TGG | 0.428487 | 6.3:+84566195 | None:intergenic |
| TACTTCTGGCTTGCAGAAGA+TGG | 0.431129 | 6.3:-84563344 | MS.gene75127:CDS |
| AAACCTTAGCAATAGTATTC+CGG | 0.434367 | 6.3:+84563755 | None:intergenic |
| TCTCTCAGTTTAACTGCTGG+TGG | 0.434390 | 6.3:+84566198 | None:intergenic |
| ATTTCCTTAGCTCTATGAGT+TGG | 0.436325 | 6.3:+84562135 | None:intergenic |
| AACCTGCCCTGCACCGATAA+GGG | 0.436795 | 6.3:+84561600 | None:intergenic |
| TCCATCATGGGGAAAGTTCT+GGG | 0.438199 | 6.3:-84564206 | MS.gene75127:CDS |
| TAACCTGCCCTGCACCGATA+AGG | 0.438248 | 6.3:+84561599 | None:intergenic |
| TTGAAGACACACTGATTGAG+AGG | 0.442774 | 6.3:-84562331 | MS.gene75127:CDS |
| CCAGAAGTAATCAGGGATTC+GGG | 0.443793 | 6.3:+84563358 | None:intergenic |
| ATGCAACTACAGGAGATAAC+AGG | 0.444350 | 6.3:-84561302 | MS.gene75127:intron |
| GTTTAGTGGCTATTCCATCA+TGG | 0.446920 | 6.3:-84564219 | MS.gene75127:CDS |
| TAATGCCTTCTGTCTAAGCT+TGG | 0.447789 | 6.3:+84563517 | None:intergenic |
| AAGATAGCAGAAGGAGGAGA+AGG | 0.447791 | 6.3:-84571320 | MS.gene75127:CDS |
| CAAATTTAGTTCAAACTTCG+TGG | 0.454847 | 6.3:-84561633 | MS.gene75127:CDS |
| ATAGAATACCAGGATCCATC+AGG | 0.456520 | 6.3:+84562063 | None:intergenic |
| GTAATGGCGGGTTCCCTGCT+TGG | 0.457885 | 6.3:-84562509 | MS.gene75127:CDS |
| AAAATGCCAACTGATCTTGT+TGG | 0.460164 | 6.3:-84562594 | MS.gene75127:CDS |
| AGCCTCAAAATTCTTACAGT+TGG | 0.465115 | 6.3:-84562485 | MS.gene75127:CDS |
| AACCAGAATTCTGGTGGAAG+TGG | 0.466445 | 6.3:+84564052 | None:intergenic |
| TCGAAACATCTTTCCCATAT+GGG | 0.466714 | 6.3:-84561240 | MS.gene75127:CDS |
| TACTCCACAGGTACGGCTGT+TGG | 0.468900 | 6.3:-84561921 | MS.gene75127:intron |
| TGGCACTTGGTTTGCTGTAG+AGG | 0.475158 | 6.3:-84561881 | MS.gene75127:CDS |
| TGCCCATGTCATATCTATAT+GGG | 0.475927 | 6.3:-84563870 | MS.gene75127:CDS |
| AGATGGAGATTTCCCACAAC+AGG | 0.476470 | 6.3:-84561412 | MS.gene75127:intron |
| CACAAAACCCTGATGGATCC+TGG | 0.477094 | 6.3:-84562071 | MS.gene75127:intron |
| AACAGTAGATACCTTTGCAT+TGG | 0.479394 | 6.3:-84563458 | MS.gene75127:CDS |
| AACATAAGAAGGAAATTGTT+CGG | 0.480544 | 6.3:-84565786 | MS.gene75127:CDS |
| TCAATCTCAGCATGTTCTTG+TGG | 0.480961 | 6.3:+84571233 | None:intergenic |
| ATCAATTCACAGATGGAAGA+TGG | 0.483390 | 6.3:-84561429 | MS.gene75127:CDS |
| ACCCAGAACTTTCCCCATGA+TGG | 0.487722 | 6.3:+84564205 | None:intergenic |
| GGCACTTGGTTTGCTGTAGA+GGG | 0.487793 | 6.3:-84561880 | MS.gene75127:CDS |
| ATCTTTCCCATATGGGCTCT+GGG | 0.491199 | 6.3:-84561233 | MS.gene75127:CDS |
| ATGGGGATTTCTTTATCATG+TGG | 0.493396 | 6.3:-84563570 | MS.gene75127:CDS |
| ACTTCATAGGACGTCAACAC+TGG | 0.494111 | 6.3:-84571280 | MS.gene75127:CDS |
| GTCCCTTATCGGTGCAGGGC+AGG | 0.495376 | 6.3:-84561602 | MS.gene75127:intron |
| ACAATTTCCTTCTTATGTTG+AGG | 0.498096 | 6.3:+84565790 | None:intergenic |
| AATAGGTATGTTGAGTGCAC+TGG | 0.502674 | 6.3:-84562198 | MS.gene75127:intron |
| CGCTACAGAGCAGTAATGGC+GGG | 0.502708 | 6.3:-84562521 | MS.gene75127:CDS |
| CTCTCGCTACAGAGCAGTAA+TGG | 0.505047 | 6.3:-84562525 | MS.gene75127:CDS |
| CATTGATTACAACAATAAGA+AGG | 0.505283 | 6.3:-84566152 | MS.gene75127:CDS |
| TCTAGGACCTCAACATAAGA+AGG | 0.512830 | 6.3:-84565797 | MS.gene75127:CDS |
| TGAAATGAATTGCTTTGGAA+AGG | 0.513073 | 6.3:+84562103 | None:intergenic |
| TTTAGTGGCTATTCCATCAT+GGG | 0.514005 | 6.3:-84564218 | MS.gene75127:CDS |
| TCACGCTATATATAGGTACT+TGG | 0.516796 | 6.3:-84564100 | MS.gene75127:intron |
| GTGTCTTCAAAGAATTCAGT+TGG | 0.517960 | 6.3:+84562344 | None:intergenic |
| AGATAGCAGAAGGAGGAGAA+GGG | 0.517972 | 6.3:-84571319 | MS.gene75127:CDS |
| GAATTTGTGCAGACAAGACT+CGG | 0.520633 | 6.3:+84561198 | None:intergenic |
| AAAGGCCAGCAATTCAGAAA+AGG | 0.520870 | 6.3:+84563542 | None:intergenic |
| CTCCACAGGTACGGCTGTTG+GGG | 0.523437 | 6.3:-84561919 | MS.gene75127:intron |
| GACAGAACGAAGATGGTGGA+TGG | 0.523858 | 6.3:-84564381 | MS.gene75127:intron |
| ATATCTTGAATCATAGGATG+TGG | 0.524227 | 6.3:+84563596 | None:intergenic |
| TTCAAGCTCATGATGGCCAC+TGG | 0.530366 | 6.3:-84566107 | MS.gene75127:CDS |
| GTCTCTGACTGCACCGCAGA+AGG | 0.530369 | 6.3:-84562744 | MS.gene75127:CDS |
| CTACACTAATTACCTGTTGT+GGG | 0.532832 | 6.3:+84561400 | None:intergenic |
| ACAGAACGAAGATGGTGGAT+GGG | 0.533685 | 6.3:-84564380 | MS.gene75127:intron |
| GCTACACTAATTACCTGTTG+TGG | 0.534062 | 6.3:+84561399 | None:intergenic |
| TATATTGCACTGAGGCTGCT+TGG | 0.534774 | 6.3:-84564310 | MS.gene75127:CDS |
| TCTATATGGGAAGAAGTTTG+TGG | 0.537189 | 6.3:-84563857 | MS.gene75127:CDS |
| AAATCAACTGGAACAAAGCC+CGG | 0.537569 | 6.3:-84563774 | MS.gene75127:CDS |
| TCGATATGATGAGTAGTTCA+GGG | 0.538351 | 6.3:+84561259 | None:intergenic |
| TTTGCAAGGAAGGACTGTTA+CGG | 0.538358 | 6.3:+84561833 | None:intergenic |
| AACTTCATTCCACGGTGTAT+GGG | 0.539799 | 6.3:+84561456 | None:intergenic |
| AAGAACATGCTGAGATTGAA+AGG | 0.540342 | 6.3:-84571229 | MS.gene75127:CDS |
| TACTCTCCCAGAGCCCATAT+GGG | 0.543860 | 6.3:+84561227 | None:intergenic |
| TGTTGTCCCTTATCGGTGCA+GGG | 0.544685 | 6.3:-84561606 | MS.gene75127:CDS |
| AGATAACAGGAGTATTTATG+AGG | 0.545080 | 6.3:-84561289 | MS.gene75127:CDS |
| GTTAATAATCAATTCACAGA+TGG | 0.545863 | 6.3:-84561436 | MS.gene75127:CDS |
| TGTAACGTGAGTGAAGAGTA+TGG | 0.547729 | 6.3:-84562903 | MS.gene75127:CDS |
| TGGGGATTTCTTTATCATGT+GGG | 0.548896 | 6.3:-84563569 | MS.gene75127:CDS |
| TGGGGAATTTGCTATACGTA+TGG | 0.549854 | 6.3:-84561901 | MS.gene75127:CDS |
| GCACTGAGGCTGCTTGGAGA+AGG | 0.550449 | 6.3:-84564304 | MS.gene75127:CDS |
| CTTGGAGAAGGACCTGAAGA+TGG | 0.550707 | 6.3:-84564292 | MS.gene75127:CDS |
| AAATTTAGTTCAAACTTCGT+GGG | 0.551738 | 6.3:-84561632 | MS.gene75127:CDS |
| AGCCCGGAATACTATTGCTA+AGG | 0.551892 | 6.3:-84563758 | MS.gene75127:intron |
| TCTCAATGCATGACCAAGGT+TGG | 0.552996 | 6.3:-84562770 | MS.gene75127:CDS |
| CAGAACGAAGATGGTGGATG+GGG | 0.555253 | 6.3:-84564379 | MS.gene75127:CDS |
| AGAAGATGGCTTGAAAATCC+AGG | 0.558427 | 6.3:-84563330 | MS.gene75127:intron |
| CTATATGGGAAGAAGTTTGT+GGG | 0.559860 | 6.3:-84563856 | MS.gene75127:CDS |
| TTTGCTATACGTATGGCACT+TGG | 0.562241 | 6.3:-84561894 | MS.gene75127:CDS |
| ATTTGATTAGGTCAGTGATA+GGG | 0.563688 | 6.3:+84563833 | None:intergenic |
| TGGAAGTTGAAGATAGCAGA+AGG | 0.564446 | 6.3:-84571329 | MS.gene75127:CDS |
| TGATTATTAACTTCATTCCA+CGG | 0.564716 | 6.3:+84561448 | None:intergenic |
| AAAGGGCCAGCAGATTCTGC+AGG | 0.566025 | 6.3:+84566084 | None:intergenic |
| TCCTCAATTCAAGCTCATGA+TGG | 0.566026 | 6.3:-84566114 | MS.gene75127:CDS |
| GAAAACACACAAAACCCTGA+TGG | 0.566223 | 6.3:-84562078 | MS.gene75127:CDS |
| AAGTTTGAACTAAATTTGCA+CGG | 0.573160 | 6.3:+84561638 | None:intergenic |
| ATTCCACGGTGTATGGGTGT+AGG | 0.573394 | 6.3:+84561462 | None:intergenic |
| TGGATGGGGATTCCATATAG+AGG | 0.575350 | 6.3:-84564365 | MS.gene75127:CDS |
| TGACCTCTTGATGAGAATGC+AGG | 0.575657 | 6.3:-84571159 | MS.gene75127:intron |
| TTCTGTCTAAGCTTGGTGAA+AGG | 0.576271 | 6.3:+84563524 | None:intergenic |
| GCCATCATGAGCTTGAATTG+AGG | 0.576423 | 6.3:+84566113 | None:intergenic |
| ATTGATTACAACAATAAGAA+GGG | 0.576445 | 6.3:-84566151 | MS.gene75127:CDS |
| CTTCATAGGACGTCAACACT+GGG | 0.580277 | 6.3:-84571279 | MS.gene75127:CDS |
| AGATGGTGAAGACAAGGCAA+TGG | 0.581885 | 6.3:-84564275 | MS.gene75127:CDS |
| AAATTCACCCCTATTCATCC+AGG | 0.582742 | 6.3:-84564022 | MS.gene75127:intron |
| TGGGAATTTGATCCAAATGC+AGG | 0.583989 | 6.3:-84571260 | MS.gene75127:CDS |
| CAAGGGATCCTGTAACATAG+AGG | 0.586621 | 6.3:+84565827 | None:intergenic |
| ATACCTGCATTCTCATCAAG+AGG | 0.587025 | 6.3:+84571156 | None:intergenic |
| TCATGTGCTTTCCGAAGTGT+AGG | 0.587144 | 6.3:+84562879 | None:intergenic |
| TTTCACCAAGCTTAGACAGA+AGG | 0.587774 | 6.3:-84563522 | MS.gene75127:CDS |
| GTCAAAGCAGCTTCCTAATG+GGG | 0.591159 | 6.3:-84561791 | MS.gene75127:CDS |
| GGTAGAAACCAGAATTCTGG+TGG | 0.591415 | 6.3:+84564046 | None:intergenic |
| GAGGTCCTAGAACCTCATCA+AGG | 0.592674 | 6.3:+84565809 | None:intergenic |
| CCACTAAACCACCATGGTCA+AGG | 0.595228 | 6.3:+84564233 | None:intergenic |
| CTGCAAGCCAGAAGTAATCA+GGG | 0.596867 | 6.3:+84563351 | None:intergenic |
| ATGATTCAAGATATGTTATG+GGG | 0.597243 | 6.3:-84563587 | MS.gene75127:CDS |
| CTTCCCATATAGATATGACA+TGG | 0.597880 | 6.3:+84563867 | None:intergenic |
| ACCTGAAGATGGTGAAGACA+AGG | 0.599140 | 6.3:-84564281 | MS.gene75127:CDS |
| AAGAGTATGGTCCTACACTT+CGG | 0.599344 | 6.3:-84562890 | MS.gene75127:CDS |
| CAGCAGATTCTGCAGGCCAG+TGG | 0.600116 | 6.3:+84566091 | None:intergenic |
| CTGCACCGCAGAAGGACTCA+AGG | 0.601671 | 6.3:-84562736 | MS.gene75127:intron |
| ATGGGGAAAGTTCTGGGTCA+CGG | 0.605144 | 6.3:-84564200 | MS.gene75127:intron |
| GTTTAACTGCTGGTGGAATG+GGG | 0.606958 | 6.3:+84566205 | None:intergenic |
| AGTCAGAGACTTGCCAACCT+TGG | 0.608423 | 6.3:+84562757 | None:intergenic |
| TCGGTATTTATATAATCACC+AGG | 0.608586 | 6.3:-84565767 | MS.gene75127:intron |
| ACACCCAACTCATAGAGCTA+AGG | 0.609087 | 6.3:-84562139 | MS.gene75127:CDS |
| TCTCTACACTTCCAATGCAA+AGG | 0.614361 | 6.3:+84563447 | None:intergenic |
| CTTGGAGTTTATGAGTGGCG+AGG | 0.615223 | 6.3:-84564082 | MS.gene75127:CDS |
| TCAAAGCAGCTTCCTAATGG+GGG | 0.615650 | 6.3:-84561790 | MS.gene75127:CDS |
| AAGGGATCCTGTAACATAGA+GGG | 0.615864 | 6.3:+84565828 | None:intergenic |
| GTAGAGGGACTAACAGCTTG+TGG | 0.616366 | 6.3:-84561865 | MS.gene75127:CDS |
| GGAAAATGTTGTGCTACTGT+CGG | 0.617544 | 6.3:-84563903 | MS.gene75127:CDS |
| TGAAGACACACTGATTGAGA+GGG | 0.617821 | 6.3:-84562330 | MS.gene75127:CDS |
| TTATTTGTCAAGCCAAAACA+AGG | 0.619533 | 6.3:-84561755 | MS.gene75127:intron |
| ATGAGGTGACAGAACGAAGA+TGG | 0.622105 | 6.3:-84564388 | MS.gene75127:intron |
| AATTAATCATGTGCGTTACG+AGG | 0.622234 | 6.3:-84563486 | MS.gene75127:CDS |
| GACACATATGCAAAGGATCG+TGG | 0.624587 | 6.3:-84562797 | MS.gene75127:CDS |
| GAAAGCACATGACTTCCTGA+AGG | 0.625323 | 6.3:-84562868 | MS.gene75127:intron |
| GAGCTTTGGGTGTCAAATGT+GGG | 0.627157 | 6.3:-84562958 | MS.gene75127:CDS |
| TATCTTGAATCATAGGATGT+GGG | 0.628079 | 6.3:+84563597 | None:intergenic |
| GAGAAGAAGATATCATAACA+GGG | 0.628365 | 6.3:-84566179 | MS.gene75127:CDS |
| AGGTACTTGGAGTTTATGAG+TGG | 0.631997 | 6.3:-84564087 | MS.gene75127:CDS |
| AAGTTGAAGATAGCAGAAGG+AGG | 0.632969 | 6.3:-84571326 | MS.gene75127:CDS |
| AGAGCTTTGGGTGTCAAATG+TGG | 0.632997 | 6.3:-84562959 | MS.gene75127:CDS |
| AGGTCCTAGAACCTCATCAA+GGG | 0.639948 | 6.3:+84565810 | None:intergenic |
| ATGTACAGACACATATGCAA+AGG | 0.641280 | 6.3:-84562804 | MS.gene75127:CDS |
| TAAAACCTTGAGTCCTTCTG+CGG | 0.644286 | 6.3:+84562731 | None:intergenic |
| TGTTACAGGATCCCTTGATG+AGG | 0.647790 | 6.3:-84565821 | MS.gene75127:CDS |
| TCGCTACAGAGCAGTAATGG+CGG | 0.652702 | 6.3:-84562522 | MS.gene75127:CDS |
| GTATGCACCTGGATGAATAG+GGG | 0.653207 | 6.3:+84564015 | None:intergenic |
| CATTACTGCTCTGTAGCGAG+AGG | 0.654544 | 6.3:+84562526 | None:intergenic |
| TAGAATACCAGGATCCATCA+GGG | 0.660292 | 6.3:+84562064 | None:intergenic |
| TTAGTGGCTATTCCATCATG+GGG | 0.661744 | 6.3:-84564217 | MS.gene75127:CDS |
| CAAAGCAGCTTCCTAATGGG+GGG | 0.662526 | 6.3:-84561789 | MS.gene75127:CDS |
| AATTGGATCCTTGACCATGG+TGG | 0.663800 | 6.3:-84564241 | MS.gene75127:CDS |
| GGATGGGGATTCCATATAGA+GGG | 0.664160 | 6.3:-84564364 | MS.gene75127:CDS |
| AGGTGACAGAACGAAGATGG+TGG | 0.664546 | 6.3:-84564385 | MS.gene75127:intron |
| TTCCCATATAGATATGACAT+GGG | 0.670291 | 6.3:+84563868 | None:intergenic |
| AGAAGAAGATATCATAACAG+GGG | 0.679958 | 6.3:-84566178 | MS.gene75127:CDS |
| TAGCTTTGTGAAGAGATACA+GGG | 0.686063 | 6.3:+84562161 | None:intergenic |
| GGTTGAAGACCCGAACTCAG+AGG | 0.694258 | 6.3:-84563399 | MS.gene75127:intron |
| ACATTCTCAATGCATGACCA+AGG | 0.704194 | 6.3:-84562774 | MS.gene75127:CDS |
| GAATAGCCACTAAACCACCA+TGG | 0.737768 | 6.3:+84564227 | None:intergenic |
| AGAAATTGGATCCTTGACCA+TGG | 0.742771 | 6.3:-84564244 | MS.gene75127:CDS |
| GATCCTACACCCATACACCG+TGG | 0.761975 | 6.3:-84561465 | MS.gene75127:CDS |
| CATTGAGCTATATTGCACTG+AGG | 0.768445 | 6.3:-84564318 | MS.gene75127:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 84561181 | 84571354 | 84561181 | ID=MS.gene75127 |
| chr6.3 | mRNA | 84561181 | 84571354 | 84561181 | ID=MS.gene75127.t1;Parent=MS.gene75127 |
| chr6.3 | exon | 84571160 | 84571354 | 84571160 | ID=MS.gene75127.t1.exon1;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84571160 | 84571354 | 84571160 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84566065 | 84566250 | 84566065 | ID=MS.gene75127.t1.exon2;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84566065 | 84566250 | 84566065 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84565768 | 84565857 | 84565768 | ID=MS.gene75127.t1.exon3;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84565768 | 84565857 | 84565768 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84564201 | 84564398 | 84564201 | ID=MS.gene75127.t1.exon4;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84564201 | 84564398 | 84564201 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84564023 | 84564107 | 84564023 | ID=MS.gene75127.t1.exon5;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84564023 | 84564107 | 84564023 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84563759 | 84563925 | 84563759 | ID=MS.gene75127.t1.exon6;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84563759 | 84563925 | 84563759 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84563456 | 84563633 | 84563456 | ID=MS.gene75127.t1.exon7;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84563456 | 84563633 | 84563456 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84563331 | 84563413 | 84563331 | ID=MS.gene75127.t1.exon8;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84563331 | 84563413 | 84563331 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84562869 | 84562979 | 84562869 | ID=MS.gene75127.t1.exon9;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84562869 | 84562979 | 84562869 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84562737 | 84562835 | 84562737 | ID=MS.gene75127.t1.exon10;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84562737 | 84562835 | 84562737 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84562479 | 84562634 | 84562479 | ID=MS.gene75127.t1.exon11;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84562479 | 84562634 | 84562479 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84562329 | 84562375 | 84562329 | ID=MS.gene75127.t1.exon12;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84562329 | 84562375 | 84562329 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84562072 | 84562215 | 84562072 | ID=MS.gene75127.t1.exon13;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84562072 | 84562215 | 84562072 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84561756 | 84561933 | 84561756 | ID=MS.gene75127.t1.exon14;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84561756 | 84561933 | 84561756 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84561603 | 84561683 | 84561603 | ID=MS.gene75127.t1.exon15;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84561603 | 84561683 | 84561603 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84561413 | 84561496 | 84561413 | ID=MS.gene75127.t1.exon16;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84561413 | 84561496 | 84561413 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| chr6.3 | exon | 84561181 | 84561312 | 84561181 | ID=MS.gene75127.t1.exon17;Parent=MS.gene75127.t1 |
| chr6.3 | CDS | 84561181 | 84561312 | 84561181 | ID=cds.MS.gene75127.t1;Parent=MS.gene75127.t1 |
| Gene Sequence |
| Protein sequence |
