Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75441.t1 | KYP31853.1 | 47.8 | 274 | 19 | 2 | 1 | 151 | 1042 | 1314 | 7.10E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75441.t1 | P10978 | 36.9 | 268 | 46 | 1 | 2 | 146 | 1058 | 1325 | 6.5e-41 | 168.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75441.t1 | A0A151QNH6 | 47.8 | 274 | 19 | 2 | 1 | 151 | 1042 | 1314 | 5.1e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene75441 | 0.805554 | 1.26E-49 | -1.69E-46 |
| MS.gene051597 | MS.gene75441 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene75441 | 0.803959 | 2.72E-49 | -1.69E-46 |
| MS.gene058702 | MS.gene75441 | 0.809949 | 1.47E-50 | -1.69E-46 |
| MS.gene058881 | MS.gene75441 | 0.808448 | 3.08E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene75441 | 0.809141 | 2.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTATCCTTTGATTAGTT+TGG | 0.231148 | 3.3:+73188542 | None:intergenic |
| AACTATCCTTTGATTAGTTT+GGG | 0.250379 | 3.3:+73188543 | None:intergenic |
| TGCTCATCTCTTCTTTCTCT+TGG | 0.271304 | 3.3:+73189227 | None:intergenic |
| CAAAACTCTATCTTCTCTTT+TGG | 0.321800 | 3.3:+73188593 | None:intergenic |
| TCTTTCTCTTGGTCATTCTC+AGG | 0.376250 | 3.3:+73189238 | None:intergenic |
| GCTACCCACTGCTGAAGAAT+AGG | 0.395959 | 3.3:+73189198 | None:intergenic |
| CATATCTGCTCCATTGTCAT+TGG | 0.455158 | 3.3:+73188631 | None:intergenic |
| AACACTGGAGTGCTGTGAAA+TGG | 0.463543 | 3.3:-73189090 | MS.gene75441:CDS |
| CCTGTGCATGTTCCGCTACC+TGG | 0.472224 | 3.3:-73189289 | MS.gene75441:CDS |
| CTACCCACTGCTGAAGAATA+GGG | 0.473406 | 3.3:+73189199 | None:intergenic |
| TGCAGACCATGGCGTACATA+AGG | 0.500035 | 3.3:+73189176 | None:intergenic |
| ACATCGATGTGAGGTATCAC+TGG | 0.524604 | 3.3:-73188703 | MS.gene75441:intron |
| TTGTAAAGAAGCTGCAGGCT+TGG | 0.530111 | 3.3:-73188573 | MS.gene75441:CDS |
| ATCTCAAGAAAGTTCTTGAA+AGG | 0.540106 | 3.3:-73189333 | MS.gene75441:CDS |
| CCTACAGCATAACCGATGTC+TGG | 0.542961 | 3.3:+73189148 | None:intergenic |
| CCAGACATCGGTTATGCTGT+AGG | 0.556943 | 3.3:-73189148 | MS.gene75441:CDS |
| GTCTGGCCTCGTGCAGACCA+TGG | 0.563338 | 3.3:+73189165 | None:intergenic |
| CCAGGTAGCGGAACATGCAC+AGG | 0.572979 | 3.3:+73189289 | None:intergenic |
| AAAATTCATACCAATGACAA+TGG | 0.582534 | 3.3:-73188641 | MS.gene75441:CDS |
| TTACTGAGTTTCATATGACC+AGG | 0.584762 | 3.3:+73189271 | None:intergenic |
| GTGCCCTATTCTTCAGCAGT+GGG | 0.600613 | 3.3:-73189202 | MS.gene75441:CDS |
| TCACTGGATACGTGATGTGT+TGG | 0.601006 | 3.3:-73188687 | MS.gene75441:CDS |
| GGTGCCCTATTCTTCAGCAG+TGG | 0.613159 | 3.3:-73189203 | MS.gene75441:CDS |
| ACTATCCTTTGATTAGTTTG+GGG | 0.614975 | 3.3:+73188544 | None:intergenic |
| GGGTAGCCTTATGTACGCCA+TGG | 0.620781 | 3.3:-73189182 | MS.gene75441:CDS |
| CAAACCCAGGAAAAGAACAC+TGG | 0.623987 | 3.3:-73189105 | MS.gene75441:CDS |
| AGAGAAAGAAGAGATGAGCA+AGG | 0.630245 | 3.3:-73189224 | MS.gene75441:CDS |
| GTCTGCACGAGGCCAGACAT+CGG | 0.643214 | 3.3:-73189160 | MS.gene75441:CDS |
| ATCGTTCAAGAAGAATGTTG+TGG | 0.658412 | 3.3:-73189372 | None:intergenic |
| AGTAGATTCCTATCAAACCC+AGG | 0.660954 | 3.3:-73189118 | MS.gene75441:CDS |
| AGTTTCATATGACCAGGTAG+CGG | 0.678545 | 3.3:+73189277 | None:intergenic |
| TGTACGCCATGGTCTGCACG+AGG | 0.697121 | 3.3:-73189171 | MS.gene75441:CDS |
| GTGCTGTGAAATGGATACTG+AGG | 0.727600 | 3.3:-73189081 | MS.gene75441:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCATACCAATGACAA+TGG | - | chr3.3:73189273-73189292 | MS.gene75441:CDS | 25.0% |
| ! | TGCAAAAGTAATCAAATATC+CGG | + | chr3.3:73188971-73188990 | None:intergenic | 25.0% |
| !!! | ATATTTGATTACTTTTGCAG+GGG | - | chr3.3:73188972-73188991 | MS.gene75441:intron | 25.0% |
| !!! | GATATTTGATTACTTTTGCA+GGG | - | chr3.3:73188971-73188990 | MS.gene75441:intron | 25.0% |
| ATCTCAAGAAAGTTCTTGAA+AGG | - | chr3.3:73188581-73188600 | MS.gene75441:CDS | 30.0% | |
| GAACATAACTTTCTTGATTC+TGG | + | chr3.3:73189125-73189144 | None:intergenic | 30.0% | |
| ! | CAAAACTCTATCTTCTCTTT+TGG | + | chr3.3:73189324-73189343 | None:intergenic | 30.0% |
| ! | TTTTCTTGAATCTACATCTC+CGG | + | chr3.3:73188941-73188960 | None:intergenic | 30.0% |
| !! | GGATATTTGATTACTTTTGC+AGG | - | chr3.3:73188970-73188989 | MS.gene75441:intron | 30.0% |
| !! | TATTTGATTACTTTTGCAGG+GGG | - | chr3.3:73188973-73188992 | MS.gene75441:intron | 30.0% |
| AGTAATCAAATATCCGGATG+TGG | + | chr3.3:73188965-73188984 | None:intergenic | 35.0% | |
| GATTCAAGAAAATCCACATC+CGG | - | chr3.3:73188949-73188968 | MS.gene75441:intron | 35.0% | |
| TGGTTATGTAGATGCTGATA+TGG | - | chr3.3:73188915-73188934 | MS.gene75441:intron | 35.0% | |
| TTACTGAGTTTCATATGACC+AGG | + | chr3.3:73188646-73188665 | None:intergenic | 35.0% | |
| ! | GCCAAAACATAAGCACTTTT+TGG | + | chr3.3:73188875-73188894 | None:intergenic | 35.0% |
| ! | TGGATACTGAGGTATCTAAA+AGG | - | chr3.3:73188844-73188863 | MS.gene75441:intron | 35.0% |
| !!! | AAGTGCTTATGTTTTGGCAA+TGG | - | chr3.3:73188877-73188896 | MS.gene75441:intron | 35.0% |
| !!! | GCCAAAAAGTGCTTATGTTT+TGG | - | chr3.3:73188871-73188890 | MS.gene75441:intron | 35.0% |
| AAGAAGTTCCTGCAAGAGTT+AGG | - | chr3.3:73189099-73189118 | MS.gene75441:CDS | 40.0% | |
| AATGGAAATCAAATGCTCGC+TGG | - | chr3.3:73188895-73188914 | MS.gene75441:intron | 40.0% | |
| AGAACTCGACATACCATTCA+AGG | - | chr3.3:73189181-73189200 | MS.gene75441:CDS | 40.0% | |
| AGAGAAAGAAGAGATGAGCA+AGG | - | chr3.3:73188690-73188709 | MS.gene75441:CDS | 40.0% | |
| AGTAGATTCCTATCAAACCC+AGG | - | chr3.3:73188796-73188815 | MS.gene75441:intron | 40.0% | |
| AGTTTCATATGACCAGGTAG+CGG | + | chr3.3:73188640-73188659 | None:intergenic | 40.0% | |
| CATATCTGCTCCATTGTCAT+TGG | + | chr3.3:73189286-73189305 | None:intergenic | 40.0% | |
| TATGTAGATGCTGATATGGC+CGG | - | chr3.3:73188919-73188938 | MS.gene75441:intron | 40.0% | |
| TCTTTCTCTTGGTCATTCTC+AGG | + | chr3.3:73188679-73188698 | None:intergenic | 40.0% | |
| TGCTCATCTCTTCTTTCTCT+TGG | + | chr3.3:73188690-73188709 | None:intergenic | 40.0% | |
| ! | GTTCTTTTCCTGGGTTTGAT+AGG | + | chr3.3:73188807-73188826 | None:intergenic | 40.0% |
| !! | GAGTTTTGTAAAGAAGCTGC+AGG | - | chr3.3:73189336-73189355 | MS.gene75441:CDS | 40.0% |
| AAGGTTGTAAGGAGATCCTG+TGG | - | chr3.3:73189073-73189092 | MS.gene75441:intron | 45.0% | |
| CAAACCCAGGAAAAGAACAC+TGG | - | chr3.3:73188809-73188828 | MS.gene75441:intron | 45.0% | |
| CGATGTGTTTCGACCTTGAA+TGG | + | chr3.3:73189197-73189216 | None:intergenic | 45.0% | |
| CGCAATCACTGAAGGTTGTA+AGG | - | chr3.3:73189062-73189081 | MS.gene75441:intron | 45.0% | |
| CTACCCACTGCTGAAGAATA+GGG | + | chr3.3:73188718-73188737 | None:intergenic | 45.0% | |
| GAGTACATCGCAATCACTGA+AGG | - | chr3.3:73189054-73189073 | MS.gene75441:intron | 45.0% | |
| GTGCTGTGAAATGGATACTG+AGG | - | chr3.3:73188833-73188852 | MS.gene75441:intron | 45.0% | |
| TCACTGGATACGTGATGTGT+TGG | - | chr3.3:73189227-73189246 | MS.gene75441:CDS | 45.0% | |
| TGCTGAGGTGAATTGCACTT+TGG | + | chr3.3:73189161-73189180 | None:intergenic | 45.0% | |
| TTGTAAAGAAGCTGCAGGCT+TGG | - | chr3.3:73189341-73189360 | MS.gene75441:CDS | 45.0% | |
| ! | ACATCGATGTGAGGTATCAC+TGG | - | chr3.3:73189211-73189230 | MS.gene75441:CDS | 45.0% |
| ! | CACTCCAGTGTTCTTTTCCT+GGG | + | chr3.3:73188816-73188835 | None:intergenic | 45.0% |
| !! | AACACTGGAGTGCTGTGAAA+TGG | - | chr3.3:73188824-73188843 | MS.gene75441:intron | 45.0% |
| CCTACAGCATAACCGATGTC+TGG | + | chr3.3:73188769-73188788 | None:intergenic | 50.0% | |
| GATTCTGGCCTAACTCTTGC+AGG | + | chr3.3:73189110-73189129 | None:intergenic | 50.0% | |
| GCAGGAACTTCTTCATCCAC+AGG | + | chr3.3:73189092-73189111 | None:intergenic | 50.0% | |
| GCTACCCACTGCTGAAGAAT+AGG | + | chr3.3:73188719-73188738 | None:intergenic | 50.0% | |
| GGTATGTCGAGTTCTTGCTG+AGG | + | chr3.3:73189176-73189195 | None:intergenic | 50.0% | |
| GGTCGAAACACATCGATGTG+AGG | - | chr3.3:73189202-73189221 | MS.gene75441:CDS | 50.0% | |
| GTGCCCTATTCTTCAGCAGT+GGG | - | chr3.3:73188712-73188731 | MS.gene75441:intron | 50.0% | |
| TGCAGACCATGGCGTACATA+AGG | + | chr3.3:73188741-73188760 | None:intergenic | 50.0% | |
| TGCGATGTACTCTGCTTCTG+TGG | + | chr3.3:73189046-73189065 | None:intergenic | 50.0% | |
| ! | CCAGACATCGGTTATGCTGT+AGG | - | chr3.3:73188766-73188785 | MS.gene75441:intron | 50.0% |
| ! | GCACTCCAGTGTTCTTTTCC+TGG | + | chr3.3:73188817-73188836 | None:intergenic | 50.0% |
| GGTGCCCTATTCTTCAGCAG+TGG | - | chr3.3:73188711-73188730 | MS.gene75441:intron | 55.0% | |
| !! | GGGTAGCCTTATGTACGCCA+TGG | - | chr3.3:73188732-73188751 | MS.gene75441:intron | 55.0% |
| CCTGTGCATGTTCCGCTACC+TGG | - | chr3.3:73188625-73188644 | MS.gene75441:CDS | 60.0% | |
| GTCTGCACGAGGCCAGACAT+CGG | - | chr3.3:73188754-73188773 | MS.gene75441:intron | 60.0% | |
| TGTACGCCATGGTCTGCACG+AGG | - | chr3.3:73188743-73188762 | MS.gene75441:intron | 60.0% | |
| TTGCAGGGGGAGCTGTCTCA+TGG | - | chr3.3:73188986-73189005 | MS.gene75441:intron | 60.0% | |
| !! | CCAGGTAGCGGAACATGCAC+AGG | + | chr3.3:73188628-73188647 | None:intergenic | 60.0% |
| GTCTGGCCTCGTGCAGACCA+TGG | + | chr3.3:73188752-73188771 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 73188556 | 73189380 | 73188556 | ID=MS.gene75441 |
| chr3.3 | mRNA | 73188556 | 73189380 | 73188556 | ID=MS.gene75441.t1;Parent=MS.gene75441 |
| chr3.3 | exon | 73189082 | 73189380 | 73189082 | ID=MS.gene75441.t1.exon1;Parent=MS.gene75441.t1 |
| chr3.3 | CDS | 73189082 | 73189380 | 73189082 | ID=cds.MS.gene75441.t1;Parent=MS.gene75441.t1 |
| chr3.3 | exon | 73188556 | 73188712 | 73188556 | ID=MS.gene75441.t1.exon2;Parent=MS.gene75441.t1 |
| chr3.3 | CDS | 73188556 | 73188712 | 73188556 | ID=cds.MS.gene75441.t1;Parent=MS.gene75441.t1 |
| Gene Sequence |
| Protein sequence |
