Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058702.t1 | KYP31853.1 | 48.2 | 274 | 18 | 2 | 1 | 151 | 1042 | 1314 | 1.40E-55 | 225.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058702.t1 | P10978 | 36.9 | 268 | 46 | 1 | 2 | 146 | 1058 | 1325 | 6.5e-41 | 168.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058702.t1 | A0A151QNH6 | 48.2 | 274 | 18 | 2 | 1 | 151 | 1042 | 1314 | 1.0e-55 | 225.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058702 | MS.gene31429 | 0.806291 | 8.84E-50 | -1.69E-46 |
| MS.gene058702 | MS.gene75441 | 0.809949 | 1.47E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTATCCTTTGATTAGTTT+GGG | 0.219958 | 7.4:-26443204 | None:intergenic |
| AAACTATCCTTTGATTAGTT+TGG | 0.231148 | 7.4:-26443205 | None:intergenic |
| TGCTCATCTCTTCTTTCTCT+TGG | 0.271304 | 7.4:-26442520 | None:intergenic |
| TCTTTCTCTTGGTCATTCTC+TGG | 0.317966 | 7.4:-26442509 | None:intergenic |
| CAAAACTCTATCTTCTCTTT+TGG | 0.321800 | 7.4:-26443154 | None:intergenic |
| GCTACCCACTGCTGAAGAAT+AGG | 0.395959 | 7.4:-26442549 | None:intergenic |
| AACATTGGAGTGCTGTGAAA+TGG | 0.453914 | 7.4:+26442657 | MS.gene058702:CDS |
| CATATCTGCTCCATTGTCAT+TGG | 0.455158 | 7.4:-26443116 | None:intergenic |
| CCTGTGCATGTTCCGCTACC+TGG | 0.472224 | 7.4:+26442458 | MS.gene058702:CDS |
| CTACCCACTGCTGAAGAATA+GGG | 0.473406 | 7.4:-26442548 | None:intergenic |
| TGCAGACCATGGCGTACATA+AGG | 0.500035 | 7.4:-26442571 | None:intergenic |
| ACCTCAAGAAAGTTCTTGAA+AGG | 0.504136 | 7.4:+26442414 | MS.gene058702:CDS |
| ACATCGATGTGAGGTATCAC+TGG | 0.524604 | 7.4:+26443044 | MS.gene058702:intron |
| TTGTAAAGAAGCTGCAGGCT+TGG | 0.530111 | 7.4:+26443174 | MS.gene058702:CDS |
| CCTACAGCATAACCGATGTC+TGG | 0.542961 | 7.4:-26442599 | None:intergenic |
| CCAGACATCGGTTATGCTGT+AGG | 0.556943 | 7.4:+26442599 | MS.gene058702:CDS |
| GTCTGGCCTCGTGCAGACCA+TGG | 0.563338 | 7.4:-26442582 | None:intergenic |
| CAAACCCAGGAAAAGAACAT+TGG | 0.572677 | 7.4:+26442642 | MS.gene058702:CDS |
| CCAGGTAGCGGAACATGCAC+AGG | 0.572979 | 7.4:-26442458 | None:intergenic |
| AAAATTCATACCAATGACAA+TGG | 0.582534 | 7.4:+26443106 | MS.gene058702:CDS |
| TTACTGAGTTTCATATGACC+AGG | 0.584762 | 7.4:-26442476 | None:intergenic |
| ACCTTTCAAGAACTTTCTTG+AGG | 0.592111 | 7.4:-26442415 | None:intergenic |
| GTGCCCTATTCTTCAGCAGT+GGG | 0.600613 | 7.4:+26442545 | MS.gene058702:CDS |
| TCACTGGATACGTGATGTGT+TGG | 0.601006 | 7.4:+26443060 | MS.gene058702:CDS |
| GGTGCCCTATTCTTCAGCAG+TGG | 0.613159 | 7.4:+26442544 | MS.gene058702:CDS |
| ACTATCCTTTGATTAGTTTG+GGG | 0.614975 | 7.4:-26443203 | None:intergenic |
| GGGTAGCCTTATGTACGCCA+TGG | 0.620781 | 7.4:+26442565 | MS.gene058702:CDS |
| AGAGAAAGAAGAGATGAGCA+AGG | 0.630245 | 7.4:+26442523 | MS.gene058702:CDS |
| GTCTGCACGAGGCCAGACAT+CGG | 0.643214 | 7.4:+26442587 | MS.gene058702:CDS |
| AGTAGATTCCTATCAAACCC+AGG | 0.660954 | 7.4:+26442629 | MS.gene058702:CDS |
| AGTTTCATATGACCAGGTAG+CGG | 0.678545 | 7.4:-26442470 | None:intergenic |
| TGTACGCCATGGTCTGCACG+AGG | 0.697121 | 7.4:+26442576 | MS.gene058702:CDS |
| GTGCTGTGAAATGGATACTG+AGG | 0.727600 | 7.4:+26442666 | MS.gene058702:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCATACCAATGACAA+TGG | + | chr7.4:26443106-26443125 | MS.gene058702:CDS | 25.0% |
| ! | TGCAAAAGTAATCAAATATC+CGG | - | chr7.4:26442804-26442823 | None:intergenic | 25.0% |
| !!! | ATATTTGATTACTTTTGCAG+GGG | + | chr7.4:26442805-26442824 | MS.gene058702:intron | 25.0% |
| !!! | GATATTTGATTACTTTTGCA+GGG | + | chr7.4:26442804-26442823 | MS.gene058702:intron | 25.0% |
| GAACATAACTTTCTTGATTC+TGG | - | chr7.4:26442958-26442977 | None:intergenic | 30.0% | |
| ! | CAAAACTCTATCTTCTCTTT+TGG | - | chr7.4:26443157-26443176 | None:intergenic | 30.0% |
| ! | TTTTCTTGAATCTACATCTC+CGG | - | chr7.4:26442774-26442793 | None:intergenic | 30.0% |
| !! | GGATATTTGATTACTTTTGC+AGG | + | chr7.4:26442803-26442822 | MS.gene058702:intron | 30.0% |
| !! | TATTTGATTACTTTTGCAGG+GGG | + | chr7.4:26442806-26442825 | MS.gene058702:intron | 30.0% |
| ACCTCAAGAAAGTTCTTGAA+AGG | + | chr7.4:26442414-26442433 | MS.gene058702:CDS | 35.0% | |
| ACCTTTCAAGAACTTTCTTG+AGG | - | chr7.4:26442418-26442437 | None:intergenic | 35.0% | |
| AGAATTCGACATACCATTCA+AGG | + | chr7.4:26443014-26443033 | MS.gene058702:intron | 35.0% | |
| AGTAATCAAATATCCGGATG+TGG | - | chr7.4:26442798-26442817 | None:intergenic | 35.0% | |
| GATTCAAGAAAATCCACATC+CGG | + | chr7.4:26442782-26442801 | MS.gene058702:intron | 35.0% | |
| TGGTTATGTAGATGCTGATA+TGG | + | chr7.4:26442748-26442767 | MS.gene058702:intron | 35.0% | |
| TTACTGAGTTTCATATGACC+AGG | - | chr7.4:26442479-26442498 | None:intergenic | 35.0% | |
| ! | GCCAAAACATAAGCACTTTT+TGG | - | chr7.4:26442708-26442727 | None:intergenic | 35.0% |
| ! | TGGATACTGAGGTATCTAAA+AGG | + | chr7.4:26442677-26442696 | MS.gene058702:intron | 35.0% |
| !!! | AAGTGCTTATGTTTTGGCAA+TGG | + | chr7.4:26442710-26442729 | MS.gene058702:intron | 35.0% |
| !!! | GCCAAAAAGTGCTTATGTTT+TGG | + | chr7.4:26442704-26442723 | MS.gene058702:intron | 35.0% |
| AAGAAGTTCCTGCAAGAGTT+AGG | + | chr7.4:26442932-26442951 | MS.gene058702:intron | 40.0% | |
| AATGGAAATCAAATGCTCGC+TGG | + | chr7.4:26442728-26442747 | MS.gene058702:intron | 40.0% | |
| AGAGAAAGAAGAGATGAGCA+AGG | + | chr7.4:26442523-26442542 | MS.gene058702:CDS | 40.0% | |
| AGTAGATTCCTATCAAACCC+AGG | + | chr7.4:26442629-26442648 | MS.gene058702:CDS | 40.0% | |
| AGTTTCATATGACCAGGTAG+CGG | - | chr7.4:26442473-26442492 | None:intergenic | 40.0% | |
| CAAACCCAGGAAAAGAACAT+TGG | + | chr7.4:26442642-26442661 | MS.gene058702:CDS | 40.0% | |
| CATATCTGCTCCATTGTCAT+TGG | - | chr7.4:26443119-26443138 | None:intergenic | 40.0% | |
| TATGTAGATGCTGATATGGC+CGG | + | chr7.4:26442752-26442771 | MS.gene058702:intron | 40.0% | |
| TCTTTCTCTTGGTCATTCTC+TGG | - | chr7.4:26442512-26442531 | None:intergenic | 40.0% | |
| TGCTCATCTCTTCTTTCTCT+TGG | - | chr7.4:26442523-26442542 | None:intergenic | 40.0% | |
| ! | CACTCCAATGTTCTTTTCCT+GGG | - | chr7.4:26442649-26442668 | None:intergenic | 40.0% |
| ! | GTTCTTTTCCTGGGTTTGAT+AGG | - | chr7.4:26442640-26442659 | None:intergenic | 40.0% |
| !! | AACATTGGAGTGCTGTGAAA+TGG | + | chr7.4:26442657-26442676 | MS.gene058702:CDS | 40.0% |
| !! | GAGTTTTGTAAAGAAGCTGC+AGG | + | chr7.4:26443169-26443188 | MS.gene058702:CDS | 40.0% |
| !!! | CGATGTGTTTTGACCTTGAA+TGG | - | chr7.4:26443030-26443049 | None:intergenic | 40.0% |
| AAGGTTGTAAGGAGATCCTG+TGG | + | chr7.4:26442906-26442925 | MS.gene058702:intron | 45.0% | |
| CGCAATCACTGAAGGTTGTA+AGG | + | chr7.4:26442895-26442914 | MS.gene058702:intron | 45.0% | |
| CTACCCACTGCTGAAGAATA+GGG | - | chr7.4:26442551-26442570 | None:intergenic | 45.0% | |
| GAGTACATCGCAATCACTGA+AGG | + | chr7.4:26442887-26442906 | MS.gene058702:intron | 45.0% | |
| GGTATGTCGAATTCTTGCTG+AGG | - | chr7.4:26443009-26443028 | None:intergenic | 45.0% | |
| GGTCAAAACACATCGATGTG+AGG | + | chr7.4:26443035-26443054 | MS.gene058702:intron | 45.0% | |
| GTGCTGTGAAATGGATACTG+AGG | + | chr7.4:26442666-26442685 | MS.gene058702:CDS | 45.0% | |
| TCACTGGATACGTGATGTGT+TGG | + | chr7.4:26443060-26443079 | MS.gene058702:CDS | 45.0% | |
| TGCTGAGGTGAATTGCACTT+TGG | - | chr7.4:26442994-26443013 | None:intergenic | 45.0% | |
| TTGTAAAGAAGCTGCAGGCT+TGG | + | chr7.4:26443174-26443193 | MS.gene058702:CDS | 45.0% | |
| ! | ACATCGATGTGAGGTATCAC+TGG | + | chr7.4:26443044-26443063 | MS.gene058702:intron | 45.0% |
| ! | GCACTCCAATGTTCTTTTCC+TGG | - | chr7.4:26442650-26442669 | None:intergenic | 45.0% |
| CCTACAGCATAACCGATGTC+TGG | - | chr7.4:26442602-26442621 | None:intergenic | 50.0% | |
| GATTCTGGCCTAACTCTTGC+AGG | - | chr7.4:26442943-26442962 | None:intergenic | 50.0% | |
| GCAGGAACTTCTTCATCCAC+AGG | - | chr7.4:26442925-26442944 | None:intergenic | 50.0% | |
| GCTACCCACTGCTGAAGAAT+AGG | - | chr7.4:26442552-26442571 | None:intergenic | 50.0% | |
| GTGCCCTATTCTTCAGCAGT+GGG | + | chr7.4:26442545-26442564 | MS.gene058702:CDS | 50.0% | |
| TGCAGACCATGGCGTACATA+AGG | - | chr7.4:26442574-26442593 | None:intergenic | 50.0% | |
| TGCGATGTACTCTGCTTCTG+TGG | - | chr7.4:26442879-26442898 | None:intergenic | 50.0% | |
| ! | CCAGACATCGGTTATGCTGT+AGG | + | chr7.4:26442599-26442618 | MS.gene058702:CDS | 50.0% |
| GGTGCCCTATTCTTCAGCAG+TGG | + | chr7.4:26442544-26442563 | MS.gene058702:CDS | 55.0% | |
| !! | GGGTAGCCTTATGTACGCCA+TGG | + | chr7.4:26442565-26442584 | MS.gene058702:CDS | 55.0% |
| CCTGTGCATGTTCCGCTACC+TGG | + | chr7.4:26442458-26442477 | MS.gene058702:CDS | 60.0% | |
| GTCTGCACGAGGCCAGACAT+CGG | + | chr7.4:26442587-26442606 | MS.gene058702:CDS | 60.0% | |
| TGTACGCCATGGTCTGCACG+AGG | + | chr7.4:26442576-26442595 | MS.gene058702:CDS | 60.0% | |
| TTGCAGGGGGAGCTGTCTCA+TGG | + | chr7.4:26442819-26442838 | MS.gene058702:intron | 60.0% | |
| !! | CCAGGTAGCGGAACATGCAC+AGG | - | chr7.4:26442461-26442480 | None:intergenic | 60.0% |
| GTCTGGCCTCGTGCAGACCA+TGG | - | chr7.4:26442585-26442604 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 26442389 | 26443213 | 26442389 | ID=MS.gene058702 |
| chr7.4 | mRNA | 26442389 | 26443213 | 26442389 | ID=MS.gene058702.t1;Parent=MS.gene058702 |
| chr7.4 | exon | 26442389 | 26442687 | 26442389 | ID=MS.gene058702.t1.exon1;Parent=MS.gene058702.t1 |
| chr7.4 | CDS | 26442389 | 26442687 | 26442389 | ID=cds.MS.gene058702.t1;Parent=MS.gene058702.t1 |
| chr7.4 | exon | 26443057 | 26443213 | 26443057 | ID=MS.gene058702.t1.exon2;Parent=MS.gene058702.t1 |
| chr7.4 | CDS | 26443057 | 26443213 | 26443057 | ID=cds.MS.gene058702.t1;Parent=MS.gene058702.t1 |
| Gene Sequence |
| Protein sequence |
