Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75762.t1 | AES73844.1 | 94.9 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.40E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75762.t1 | G7J2X5 | 94.9 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05985 | MS.gene75762 | -0.826702 | 2.35E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75762.t1 | MTR_3g109430 | 94.915 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.09e-72 | 212 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCGACCAACTAAATATTT+AGG | 0.285504 | 3.3:-90040432 | None:intergenic |
| AAACAGTATGGCAGAAGATT+TGG | 0.311355 | 3.3:+90040217 | MS.gene75762:CDS |
| AACGAACGAGGGAGATTATC+TGG | 0.376163 | 3.3:-90040372 | None:intergenic |
| TTACCACCTAAATATTTAGT+TGG | 0.429402 | 3.3:+90040426 | MS.gene75762:CDS |
| CCATCTTCAACTATAGTAGA+AGG | 0.451427 | 3.3:-90040285 | None:intergenic |
| TTGCTACCATCATAGTTGCT+TGG | 0.451500 | 3.3:-90040192 | None:intergenic |
| CTCGGTATGCTGATCCATCT+TGG | 0.452007 | 3.3:+90040400 | MS.gene75762:CDS |
| GATATGAAGAAGTACATGTT+TGG | 0.463772 | 3.3:+90040102 | MS.gene75762:CDS |
| TCAAGAAGAGAAGCAAGGTT+AGG | 0.500253 | 3.3:+90040126 | MS.gene75762:CDS |
| TACTCAATGAACTTTGATCA+TGG | 0.501867 | 3.3:+90040333 | MS.gene75762:CDS |
| ATGAACTTTGATCATGGAGC+AGG | 0.506680 | 3.3:+90040339 | MS.gene75762:CDS |
| TCCCTCGTTCGTTCTCAGCT+CGG | 0.509690 | 3.3:+90040382 | MS.gene75762:CDS |
| CCTTCTACTATAGTTGAAGA+TGG | 0.515448 | 3.3:+90040285 | MS.gene75762:CDS |
| GAAGAGAAGCAAGGTTAGGT+AGG | 0.539622 | 3.3:+90040130 | MS.gene75762:CDS |
| TGATCATGGAGCAGGTTGGA+TGG | 0.546024 | 3.3:+90040347 | MS.gene75762:CDS |
| ATTTCACCAAGCAACTATGA+TGG | 0.581383 | 3.3:+90040186 | MS.gene75762:CDS |
| TTAGGTGGTAAGATCCAAGA+TGG | 0.602511 | 3.3:-90040414 | None:intergenic |
| CGACCAACTAAATATTTAGG+TGG | 0.616235 | 3.3:-90040429 | None:intergenic |
| TCAACTATAGTAGAAGGAGA+AGG | 0.616257 | 3.3:-90040279 | None:intergenic |
| TACCGAGCTGAGAACGAACG+AGG | 0.628756 | 3.3:-90040384 | None:intergenic |
| ACTTTGATCATGGAGCAGGT+TGG | 0.656134 | 3.3:+90040343 | MS.gene75762:CDS |
| AGGTAGGAGCTACACAACAA+AGG | 0.677040 | 3.3:+90040146 | MS.gene75762:CDS |
| TTGGCTCAAGAAGAGAAGCA+AGG | 0.681319 | 3.3:+90040121 | MS.gene75762:CDS |
| ACCGAGCTGAGAACGAACGA+GGG | 0.691206 | 3.3:-90040383 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTACCACCTAAATATTTAGT+TGG | + | chr3.3:90040426-90040445 | MS.gene75762:CDS | 25.0% |
| ATGGTAGCAAAAAAACAGTA+TGG | + | chr3.3:90040205-90040224 | MS.gene75762:CDS | 30.0% | |
| CATCGACCAACTAAATATTT+AGG | - | chr3.3:90040435-90040454 | None:intergenic | 30.0% | |
| TACTCAATGAACTTTGATCA+TGG | + | chr3.3:90040333-90040352 | MS.gene75762:CDS | 30.0% | |
| ! | GATATGAAGAAGTACATGTT+TGG | + | chr3.3:90040102-90040121 | MS.gene75762:CDS | 30.0% |
| AAACAGTATGGCAGAAGATT+TGG | + | chr3.3:90040217-90040236 | MS.gene75762:CDS | 35.0% | |
| ATTTCACCAAGCAACTATGA+TGG | + | chr3.3:90040186-90040205 | MS.gene75762:CDS | 35.0% | |
| CCATCTTCAACTATAGTAGA+AGG | - | chr3.3:90040288-90040307 | None:intergenic | 35.0% | |
| CCTTCTACTATAGTTGAAGA+TGG | + | chr3.3:90040285-90040304 | MS.gene75762:CDS | 35.0% | |
| CGACCAACTAAATATTTAGG+TGG | - | chr3.3:90040432-90040451 | None:intergenic | 35.0% | |
| TCAACTATAGTAGAAGGAGA+AGG | - | chr3.3:90040282-90040301 | None:intergenic | 35.0% | |
| ATGAACTTTGATCATGGAGC+AGG | + | chr3.3:90040339-90040358 | MS.gene75762:CDS | 40.0% | |
| TCAAGAAGAGAAGCAAGGTT+AGG | + | chr3.3:90040126-90040145 | MS.gene75762:CDS | 40.0% | |
| TTAGGTGGTAAGATCCAAGA+TGG | - | chr3.3:90040417-90040436 | None:intergenic | 40.0% | |
| TTGCTACCATCATAGTTGCT+TGG | - | chr3.3:90040195-90040214 | None:intergenic | 40.0% | |
| AACGAACGAGGGAGATTATC+TGG | - | chr3.3:90040375-90040394 | None:intergenic | 45.0% | |
| ACTTTGATCATGGAGCAGGT+TGG | + | chr3.3:90040343-90040362 | MS.gene75762:CDS | 45.0% | |
| AGGTAGGAGCTACACAACAA+AGG | + | chr3.3:90040146-90040165 | MS.gene75762:CDS | 45.0% | |
| GAAGAGAAGCAAGGTTAGGT+AGG | + | chr3.3:90040130-90040149 | MS.gene75762:CDS | 45.0% | |
| TTGGCTCAAGAAGAGAAGCA+AGG | + | chr3.3:90040121-90040140 | MS.gene75762:CDS | 45.0% | |
| CTCGGTATGCTGATCCATCT+TGG | + | chr3.3:90040400-90040419 | MS.gene75762:CDS | 50.0% | |
| ! | TGATCATGGAGCAGGTTGGA+TGG | + | chr3.3:90040347-90040366 | MS.gene75762:CDS | 50.0% |
| ACCGAGCTGAGAACGAACGA+GGG | - | chr3.3:90040386-90040405 | None:intergenic | 55.0% | |
| TACCGAGCTGAGAACGAACG+AGG | - | chr3.3:90040387-90040406 | None:intergenic | 55.0% | |
| TCCCTCGTTCGTTCTCAGCT+CGG | + | chr3.3:90040382-90040401 | MS.gene75762:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 90040099 | 90040455 | 90040099 | ID=MS.gene75762 |
| chr3.3 | mRNA | 90040099 | 90040455 | 90040099 | ID=MS.gene75762.t1;Parent=MS.gene75762 |
| chr3.3 | exon | 90040099 | 90040455 | 90040099 | ID=MS.gene75762.t1.exon1;Parent=MS.gene75762.t1 |
| chr3.3 | CDS | 90040099 | 90040455 | 90040099 | ID=cds.MS.gene75762.t1;Parent=MS.gene75762.t1 |
| Gene Sequence |
| Protein sequence |
