Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75914.t1 | AES73844.1 | 94.9 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.40E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75914.t1 | G7J2X5 | 94.9 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05985 | MS.gene75914 | -0.810586 | 1.07E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75914.t1 | MTR_3g109430 | 94.915 | 118 | 5 | 1 | 1 | 118 | 1 | 117 | 1.09e-72 | 212 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCGACCAACTAAATATTT+AGG | 0.285504 | 3.3:-90029594 | None:intergenic |
| AAACAGTATGGCAGAAGATT+TGG | 0.311355 | 3.3:+90029379 | MS.gene75914:CDS |
| AACGAACGAGGGAGATTATC+TGG | 0.376163 | 3.3:-90029534 | None:intergenic |
| TTACCACCTAAATATTTAGT+TGG | 0.429402 | 3.3:+90029588 | MS.gene75914:CDS |
| CCATCTTCAACTATAGTAGA+AGG | 0.451427 | 3.3:-90029447 | None:intergenic |
| TTGCTACCATCATAGTTGCT+TGG | 0.451500 | 3.3:-90029354 | None:intergenic |
| CTCGGTATGCTGATCCATCT+TGG | 0.452007 | 3.3:+90029562 | MS.gene75914:CDS |
| GATATGAAGAAGTACATGTT+TGG | 0.464031 | 3.3:+90029264 | MS.gene75914:CDS |
| TCAAGAAGAGAAGCAAGGTT+AGG | 0.482860 | 3.3:+90029288 | MS.gene75914:CDS |
| TACTCAATGAACTTTGATCA+TGG | 0.501867 | 3.3:+90029495 | MS.gene75914:CDS |
| ATGAACTTTGATCATGGAGC+AGG | 0.506680 | 3.3:+90029501 | MS.gene75914:CDS |
| TCCCTCGTTCGTTCTCAGCT+CGG | 0.509690 | 3.3:+90029544 | MS.gene75914:CDS |
| CCTTCTACTATAGTTGAAGA+TGG | 0.515448 | 3.3:+90029447 | MS.gene75914:CDS |
| GAAGAGAAGCAAGGTTAGGT+AGG | 0.539622 | 3.3:+90029292 | MS.gene75914:CDS |
| TGATCATGGAGCAGGTTGGA+TGG | 0.546024 | 3.3:+90029509 | MS.gene75914:CDS |
| ATTTCACCAAGCAACTATGA+TGG | 0.581383 | 3.3:+90029348 | MS.gene75914:CDS |
| TTAGGTGGTAAGATCCAAGA+TGG | 0.602511 | 3.3:-90029576 | None:intergenic |
| CGACCAACTAAATATTTAGG+TGG | 0.616235 | 3.3:-90029591 | None:intergenic |
| TCAACTATAGTAGAAGGAGA+AGG | 0.616257 | 3.3:-90029441 | None:intergenic |
| TTGGTTCAAGAAGAGAAGCA+AGG | 0.626097 | 3.3:+90029283 | MS.gene75914:CDS |
| TACCGAGCTGAGAACGAACG+AGG | 0.628756 | 3.3:-90029546 | None:intergenic |
| ACTTTGATCATGGAGCAGGT+TGG | 0.656134 | 3.3:+90029505 | MS.gene75914:CDS |
| AGGTAGGAGCTACACAACAA+AGG | 0.677040 | 3.3:+90029308 | MS.gene75914:CDS |
| ACCGAGCTGAGAACGAACGA+GGG | 0.691206 | 3.3:-90029545 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTACCACCTAAATATTTAGT+TGG | + | chr3.3:90029588-90029607 | MS.gene75914:CDS | 25.0% |
| ATGGTAGCAAAAAAACAGTA+TGG | + | chr3.3:90029367-90029386 | MS.gene75914:CDS | 30.0% | |
| CATCGACCAACTAAATATTT+AGG | - | chr3.3:90029597-90029616 | None:intergenic | 30.0% | |
| TACTCAATGAACTTTGATCA+TGG | + | chr3.3:90029495-90029514 | MS.gene75914:CDS | 30.0% | |
| ! | GATATGAAGAAGTACATGTT+TGG | + | chr3.3:90029264-90029283 | MS.gene75914:CDS | 30.0% |
| AAACAGTATGGCAGAAGATT+TGG | + | chr3.3:90029379-90029398 | MS.gene75914:CDS | 35.0% | |
| ATTTCACCAAGCAACTATGA+TGG | + | chr3.3:90029348-90029367 | MS.gene75914:CDS | 35.0% | |
| CCATCTTCAACTATAGTAGA+AGG | - | chr3.3:90029450-90029469 | None:intergenic | 35.0% | |
| CCTTCTACTATAGTTGAAGA+TGG | + | chr3.3:90029447-90029466 | MS.gene75914:CDS | 35.0% | |
| CGACCAACTAAATATTTAGG+TGG | - | chr3.3:90029594-90029613 | None:intergenic | 35.0% | |
| TCAACTATAGTAGAAGGAGA+AGG | - | chr3.3:90029444-90029463 | None:intergenic | 35.0% | |
| ATGAACTTTGATCATGGAGC+AGG | + | chr3.3:90029501-90029520 | MS.gene75914:CDS | 40.0% | |
| TCAAGAAGAGAAGCAAGGTT+AGG | + | chr3.3:90029288-90029307 | MS.gene75914:CDS | 40.0% | |
| TTAGGTGGTAAGATCCAAGA+TGG | - | chr3.3:90029579-90029598 | None:intergenic | 40.0% | |
| TTGCTACCATCATAGTTGCT+TGG | - | chr3.3:90029357-90029376 | None:intergenic | 40.0% | |
| TTGGTTCAAGAAGAGAAGCA+AGG | + | chr3.3:90029283-90029302 | MS.gene75914:CDS | 40.0% | |
| AACGAACGAGGGAGATTATC+TGG | - | chr3.3:90029537-90029556 | None:intergenic | 45.0% | |
| ACTTTGATCATGGAGCAGGT+TGG | + | chr3.3:90029505-90029524 | MS.gene75914:CDS | 45.0% | |
| AGGTAGGAGCTACACAACAA+AGG | + | chr3.3:90029308-90029327 | MS.gene75914:CDS | 45.0% | |
| GAAGAGAAGCAAGGTTAGGT+AGG | + | chr3.3:90029292-90029311 | MS.gene75914:CDS | 45.0% | |
| CTCGGTATGCTGATCCATCT+TGG | + | chr3.3:90029562-90029581 | MS.gene75914:CDS | 50.0% | |
| ! | TGATCATGGAGCAGGTTGGA+TGG | + | chr3.3:90029509-90029528 | MS.gene75914:CDS | 50.0% |
| ACCGAGCTGAGAACGAACGA+GGG | - | chr3.3:90029548-90029567 | None:intergenic | 55.0% | |
| TACCGAGCTGAGAACGAACG+AGG | - | chr3.3:90029549-90029568 | None:intergenic | 55.0% | |
| TCCCTCGTTCGTTCTCAGCT+CGG | + | chr3.3:90029544-90029563 | MS.gene75914:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 90029261 | 90029617 | 90029261 | ID=MS.gene75914 |
| chr3.3 | mRNA | 90029261 | 90029617 | 90029261 | ID=MS.gene75914.t1;Parent=MS.gene75914 |
| chr3.3 | exon | 90029261 | 90029617 | 90029261 | ID=MS.gene75914.t1.exon1;Parent=MS.gene75914.t1 |
| chr3.3 | CDS | 90029261 | 90029617 | 90029261 | ID=cds.MS.gene75914.t1;Parent=MS.gene75914.t1 |
| Gene Sequence |
| Protein sequence |
