AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76354

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCGAGGAAGTGACATTA+AGG 0.317568 8.1:+29725988 MS.gene76354:CDS
CATTCATAAACCTTGTCACT+TGG 0.462160 8.1:-29725959 None:intergenic
AAGCTCTAGAGCAATTAATC+TGG 0.476879 8.1:+29727731 MS.gene76354:CDS
AAGTAAACTTGTCTCTGAAA+AGG 0.481023 8.1:+29726856 MS.gene76354:intron
TCATAAACCTTGTCACTTGG+AGG 0.500736 8.1:-29725956 None:intergenic
AATTTATCAATCACATCAGC+AGG 0.520508 8.1:-29727700 None:intergenic
TTACCTTAATGTCACTTCCT+CGG 0.538524 8.1:-29725991 None:intergenic
GTTAGATGCTATGGAACAGT+GGG 0.539899 8.1:+29725908 None:intergenic
ATGTGATTGATAAATTTGAC+AGG 0.544982 8.1:+29727707 MS.gene76354:CDS
ACAGTGGGGAGAAGAAAAGA+TGG 0.615197 8.1:+29725923 MS.gene76354:CDS
AGTTAGATGCTATGGAACAG+TGG 0.663318 8.1:+29725907 None:intergenic
ACAAGTACCTCCAAGTGACA+AGG 0.677388 8.1:+29725949 MS.gene76354:CDS
TTAGATGCTATGGAACAGTG+GGG 0.796776 8.1:+29725909 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAAAAATCAAAAGTAT+AGG - chr8.1:29727275-29727294 None:intergenic 10.0%
!! AAAAGGAAAATAATATACAA+AGG - chr8.1:29726366-29726385 None:intergenic 15.0%
!! CTATATTATCATTATTTAAC+TGG + chr8.1:29726014-29726033 MS.gene76354:intron 15.0%
!! TACGAAAAAAATAAATAACA+TGG - chr8.1:29726907-29726926 None:intergenic 15.0%
!!! ATATAATCTGACTTATTTTT+AGG + chr8.1:29728373-29728392 MS.gene76354:intron 15.0%
!!! TGTTATTTATTTTTTTCGTA+AGG + chr8.1:29726907-29726926 MS.gene76354:intron 15.0%
!!! TTGATCATATTCTTTTTAAT+AGG + chr8.1:29726412-29726431 MS.gene76354:intron 15.0%
!! ACTATTTAGATAGAATATGA+TGG + chr8.1:29727854-29727873 MS.gene76354:intron 20.0%
!! AGAAATTTGAGAAACAAAAA+TGG - chr8.1:29727656-29727675 None:intergenic 20.0%
!! ATGATTAGAAGAAAGATATA+TGG + chr8.1:29727125-29727144 MS.gene76354:intron 20.0%
!! TAAAATACTTCAACGAAAAA+TGG - chr8.1:29727803-29727822 None:intergenic 20.0%
!! TCAAAATAACTTAAAAGTCT+TGG - chr8.1:29728011-29728030 None:intergenic 20.0%
!! TCAAATTTCATAATAGCAAA+GGG - chr8.1:29726640-29726659 None:intergenic 20.0%
!!! AAACAAAAATGGTTGTTTTA+TGG - chr8.1:29727645-29727664 None:intergenic 20.0%
!!! TTGATATGATAACATTTTAG+TGG + chr8.1:29727067-29727086 MS.gene76354:intron 20.0%
!!! TTTTGTTTCTCAAATTTCTT+AGG + chr8.1:29727657-29727676 MS.gene76354:intron 20.0%
! AAAGATAGCAAACTTCAATT+AGG - chr8.1:29726808-29726827 None:intergenic 25.0%
! AGAATATGATCAACCTAAAA+AGG - chr8.1:29726405-29726424 None:intergenic 25.0%
! ATGTGATTGATAAATTTGAC+AGG + chr8.1:29727707-29727726 MS.gene76354:CDS 25.0%
! CAAATTTCATAATAGCAAAG+GGG - chr8.1:29726639-29726658 None:intergenic 25.0%
! CTACTTGTAAATGTAACTTT+CGG + chr8.1:29727958-29727977 MS.gene76354:intron 25.0%
! CTCAAATTTCATAATAGCAA+AGG - chr8.1:29726641-29726660 None:intergenic 25.0%
! TAGATAAACAATAGTGCTAT+GGG + chr8.1:29727879-29727898 MS.gene76354:intron 25.0%
! TCACATTGAATGCAATTAAT+TGG + chr8.1:29728194-29728213 MS.gene76354:intron 25.0%
! TCTCTGTAATAAATAGCTAA+TGG + chr8.1:29728228-29728247 MS.gene76354:intron 25.0%
! TTTGTCAAAAATAACCACTA+TGG - chr8.1:29728259-29728278 None:intergenic 25.0%
!! GAGAATTTTGTAGAAGTAAA+AGG - chr8.1:29726383-29726402 None:intergenic 25.0%
!! GTGTTAATTGCTATTCTTTA+AGG - chr8.1:29726757-29726776 None:intergenic 25.0%
!! TCTACAAAATTCTCCTTTTT+AGG + chr8.1:29726389-29726408 MS.gene76354:intron 25.0%
!!! CGTTGAAGTATTTTATTAAG+TGG + chr8.1:29727808-29727827 MS.gene76354:intron 25.0%
!!! CTTTGCTATTATGAAATTTG+AGG + chr8.1:29726639-29726658 MS.gene76354:intron 25.0%
!!! GAAGTATTTTATTAAGTGGT+GGG + chr8.1:29727812-29727831 MS.gene76354:intron 25.0%
!!! TAATCTGACTTATTTTTAGG+TGG + chr8.1:29728376-29728395 MS.gene76354:intron 25.0%
!!! TAATTGAAGTTTGCTATCTT+TGG + chr8.1:29726807-29726826 MS.gene76354:intron 25.0%
!!! TATGATAACATTTTAGTGGT+TGG + chr8.1:29727071-29727090 MS.gene76354:intron 25.0%
!!! TGAAGTATTTTATTAAGTGG+TGG + chr8.1:29727811-29727830 MS.gene76354:intron 25.0%
!!! TGTGATATATCTTAGTTTTG+AGG + chr8.1:29726158-29726177 MS.gene76354:intron 25.0%
!!! TTTTTCGTAAGGTATTTTGA+TGG + chr8.1:29726918-29726937 MS.gene76354:intron 25.0%
AAAAGTTGCATAAACGAACT+AGG - chr8.1:29726244-29726263 None:intergenic 30.0%
AAAGTTGCATAAACGAACTA+GGG - chr8.1:29726243-29726262 None:intergenic 30.0%
AATTTATCAATCACATCAGC+AGG - chr8.1:29727703-29727722 None:intergenic 30.0%
ACTCAACAACTATTGATATG+TGG + chr8.1:29727572-29727591 MS.gene76354:intron 30.0%
ACTCAATAGATTGATGTGTA+TGG + chr8.1:29726282-29726301 MS.gene76354:intron 30.0%
CTAGATAAACAATAGTGCTA+TGG + chr8.1:29727878-29727897 MS.gene76354:intron 30.0%
TGAGTAGTCTAAGAATACAA+AGG + chr8.1:29728038-29728057 MS.gene76354:intron 30.0%
TTATAAAACTCTCACCAAGA+AGG + chr8.1:29726444-29726463 MS.gene76354:intron 30.0%
TTGAGTTCCTTCACTAAATT+TGG + chr8.1:29728072-29728091 MS.gene76354:intron 30.0%
TTTACTGCTGTATATTTGTG+CGG + chr8.1:29727338-29727357 MS.gene76354:intron 30.0%
! AAGTAAACTTGTCTCTGAAA+AGG + chr8.1:29726856-29726875 MS.gene76354:intron 30.0%
! CAAGAGGATCATTTATTTCT+AGG + chr8.1:29726576-29726595 MS.gene76354:intron 30.0%
! CAGAAACATGTTTTGTGTAT+TGG - chr8.1:29727186-29727205 None:intergenic 30.0%
! GGTTTCCGGTTAAATTTTTA+GGG + chr8.1:29727469-29727488 MS.gene76354:intron 30.0%
! GTGGGTATAGATTATGATTT+TGG + chr8.1:29727830-29727849 MS.gene76354:intron 30.0%
! GTTAAGTATTGCTCAAATTC+AGG + chr8.1:29726312-29726331 MS.gene76354:intron 30.0%
! TGGTTTCCGGTTAAATTTTT+AGG + chr8.1:29727468-29727487 MS.gene76354:intron 30.0%
! TGTTTTGTGTATTGGATACT+TGG - chr8.1:29727178-29727197 None:intergenic 30.0%
ACCGTCCCTAAAAATTTAAC+CGG - chr8.1:29727477-29727496 None:intergenic 35.0%
ACTTCAACGAAAAATGGTAG+AGG - chr8.1:29727797-29727816 None:intergenic 35.0%
AGCTAATGGTTATTCCATAG+TGG + chr8.1:29728242-29728261 MS.gene76354:intron 35.0%
AGTTCCTTCACTAAATTTGG+AGG + chr8.1:29728075-29728094 MS.gene76354:intron 35.0%
CATTCATAAACCTTGTCACT+TGG - chr8.1:29725962-29725981 None:intergenic 35.0%
CTCAGGTATTTAAGCTATGT+CGG + chr8.1:29727511-29727530 MS.gene76354:intron 35.0%
CTTCAACGAAAAATGGTAGA+GGG - chr8.1:29727796-29727815 None:intergenic 35.0%
GAATGTTGCAATGGAAATTC+TGG + chr8.1:29726965-29726984 MS.gene76354:intron 35.0%
GATGAGATTGGATTATGTGT+AGG + chr8.1:29726536-29726555 MS.gene76354:intron 35.0%
GTTCCTTCACTAAATTTGGA+GGG + chr8.1:29728076-29728095 MS.gene76354:intron 35.0%
TGTGTATTGGATACTTGGTT+TGG - chr8.1:29727173-29727192 None:intergenic 35.0%
TTTATGTGTCTAACTGCCAA+TGG + chr8.1:29726943-29726962 MS.gene76354:intron 35.0%
TTTCCATTGCAACATTCCAT+TGG - chr8.1:29726962-29726981 None:intergenic 35.0%
! ATTGCTCAAATTCAGGTTCT+TGG + chr8.1:29726319-29726338 MS.gene76354:intron 35.0%
! GACAAGTTTACTTGATGACA+AGG - chr8.1:29726849-29726868 None:intergenic 35.0%
! TATGAATGCATTCTTTTCCG+AGG + chr8.1:29725974-29725993 MS.gene76354:CDS 35.0%
! TGGTGCAACTGATGTATTTT+GGG + chr8.1:29726339-29726358 MS.gene76354:intron 35.0%
! TTAACCGGAAACCAATTTTC+CGG - chr8.1:29727462-29727481 None:intergenic 35.0%
! TTACCTTAATGTCACTTCCT+CGG - chr8.1:29725994-29726013 None:intergenic 35.0%
! TTGGTGCAACTGATGTATTT+TGG + chr8.1:29726338-29726357 MS.gene76354:intron 35.0%
!! AAGCTCTAGAGCAATTAATC+TGG + chr8.1:29727731-29727750 MS.gene76354:CDS 35.0%
!! AGACTGGTGTTTTGAATGAA+AGG + chr8.1:29728154-29728173 MS.gene76354:intron 35.0%
!! GACTGGTGTTTTGAATGAAA+GGG + chr8.1:29728155-29728174 MS.gene76354:intron 35.0%
!! GTTAAATTTTTAGGGACGGT+TGG + chr8.1:29727477-29727496 MS.gene76354:intron 35.0%
!! TCCGGTTAAATTTTTAGGGA+CGG + chr8.1:29727473-29727492 MS.gene76354:intron 35.0%
!!! TCTGTTTTTTTGGCCTCTTT+CGG + chr8.1:29727432-29727451 MS.gene76354:intron 35.0%
!!! TTGCTGCTGTTCTGTTTTTT+TGG + chr8.1:29727422-29727441 MS.gene76354:intron 35.0%
AATTTGGAGGGTTCAAATGC+TGG + chr8.1:29728088-29728107 MS.gene76354:intron 40.0%
AGAAGGCTTAATCGACTGAT+TGG + chr8.1:29726461-29726480 MS.gene76354:intron 40.0%
AGCTTGGAATGATGAACAAG+AGG + chr8.1:29726560-29726579 MS.gene76354:intron 40.0%
ATCAGTCGATTAAGCCTTCT+TGG - chr8.1:29726461-29726480 None:intergenic 40.0%
ATCCAATCTCATCAGTTGTG+TGG - chr8.1:29726529-29726548 None:intergenic 40.0%
ATGATTGTTGTGTTCTAGCG+TGG + chr8.1:29726997-29727016 MS.gene76354:intron 40.0%
CAGAACAGCAGCAAAACAAA+TGG - chr8.1:29727416-29727435 None:intergenic 40.0%
CGACATAGCTTAAATACCTG+AGG - chr8.1:29727513-29727532 None:intergenic 40.0%
GAACCCTCCAAATTTAGTGA+AGG - chr8.1:29728082-29728101 None:intergenic 40.0%
GAAGGCTTAATCGACTGATT+GGG + chr8.1:29726462-29726481 MS.gene76354:intron 40.0%
TACCACACAACTGATGAGAT+TGG + chr8.1:29726524-29726543 MS.gene76354:intron 40.0%
TGCTGCAGAAAACAAAGACA+AGG - chr8.1:29726779-29726798 None:intergenic 40.0%
TGGATTATGTGTAGGTAGCT+TGG + chr8.1:29726544-29726563 MS.gene76354:intron 40.0%
TTGAGTTCAAGCAAAACTCC+AGG - chr8.1:29728135-29728154 None:intergenic 40.0%
TTTCCGAGGAAGTGACATTA+AGG + chr8.1:29725988-29726007 MS.gene76354:CDS 40.0%
! GGTGCAACTGATGTATTTTG+GGG + chr8.1:29726340-29726359 MS.gene76354:intron 40.0%
! GTTTTGCTTGAACTCAAGAC+TGG + chr8.1:29728138-29728157 MS.gene76354:intron 40.0%
! TCATAAACCTTGTCACTTGG+AGG - chr8.1:29725959-29725978 None:intergenic 40.0%
!! GTCTGTTTTCTTGTCGTTTG+TGG + chr8.1:29727360-29727379 MS.gene76354:intron 40.0%
!!! CTGTTTTTTTGGCCTCTTTC+GGG + chr8.1:29727433-29727452 MS.gene76354:intron 40.0%
!!! TGTTTTTTTGGCCTCTTTCG+GGG + chr8.1:29727434-29727453 MS.gene76354:intron 40.0%
!!! TTTAGGGACGGTTGGTTTTT+AGG + chr8.1:29727485-29727504 MS.gene76354:intron 40.0%
AAACTCCAGGTACTCCAGTA+TGG - chr8.1:29728122-29728141 None:intergenic 45.0%
AAATGGTAGAGGGCAATGCA+TGG - chr8.1:29727786-29727805 None:intergenic 45.0%
ACAAGTACCTCCAAGTGACA+AGG + chr8.1:29725949-29725968 MS.gene76354:CDS 45.0%
ACAGGGGAGTTAATAAGGCT+TGG + chr8.1:29726117-29726136 MS.gene76354:intron 45.0%
ACAGTGGGGAGAAGAAAAGA+TGG + chr8.1:29725923-29725942 MS.gene76354:CDS 45.0%
CGGCAAGCAAATTCGAATCT+GGG + chr8.1:29727978-29727997 MS.gene76354:intron 45.0%
CTGCCAATGGAATGTTGCAA+TGG + chr8.1:29726956-29726975 MS.gene76354:intron 45.0%
TCGGCAAGCAAATTCGAATC+TGG + chr8.1:29727977-29727996 MS.gene76354:intron 45.0%
! GCTATGGGTTGAATTACTCG+AGG + chr8.1:29727894-29727913 MS.gene76354:intron 45.0%
! TTCTTGTCGTTTGTGGCCTT+TGG + chr8.1:29727367-29727386 MS.gene76354:intron 45.0%
!!! GGTTGGTTTTTAGGTTCCTC+AGG + chr8.1:29727494-29727513 MS.gene76354:intron 45.0%
ACAAGCCTCAACCGAACCAA+AGG - chr8.1:29727386-29727405 None:intergenic 50.0%
CCTTATTAACTCCCCTGTGC+GGG - chr8.1:29726115-29726134 None:intergenic 50.0%
CTTATTAACTCCCCTGTGCG+GGG - chr8.1:29726114-29726133 None:intergenic 50.0%
GTCGTTTGTGGCCTTTGGTT+CGG + chr8.1:29727372-29727391 MS.gene76354:intron 50.0%
! AGCATCCATACTGGAGTACC+TGG + chr8.1:29728114-29728133 MS.gene76354:intron 50.0%
! GCCTTATTAACTCCCCTGTG+CGG - chr8.1:29726116-29726135 None:intergenic 50.0%
! GGCTCCGGAAAATTGGTTTC+CGG + chr8.1:29727455-29727474 MS.gene76354:intron 50.0%
! TGCTGGTCTAGCATCCATAC+TGG + chr8.1:29728105-29728124 MS.gene76354:intron 50.0%
CCCGCACAGGGGAGTTAATA+AGG + chr8.1:29726112-29726131 MS.gene76354:intron 55.0%
CTTTCGGGGCTCCGGAAAAT+TGG + chr8.1:29727448-29727467 MS.gene76354:intron 55.0%
TGGTAGAGGGCAATGCATGG+AGG - chr8.1:29727783-29727802 None:intergenic 55.0%
! ATTTTCCGGAGCCCCGAAAG+AGG - chr8.1:29727448-29727467 None:intergenic 55.0%
!! TGTGGCCTTTGGTTCGGTTG+AGG + chr8.1:29727378-29727397 MS.gene76354:intron 55.0%
AAGCATCTCACCCCGCACAG+GGG + chr8.1:29726101-29726120 MS.gene76354:intron 60.0%
TTTGGCCTCTTTCGGGGCTC+CGG + chr8.1:29727440-29727459 MS.gene76354:intron 60.0%
! GAAGCATCTCACCCCGCACA+GGG + chr8.1:29726100-29726119 MS.gene76354:intron 60.0%
!! AGAAGCATCTCACCCCGCAC+AGG + chr8.1:29726099-29726118 MS.gene76354:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 29725918 29728407 29725918 ID=MS.gene76354
chr8.1 mRNA 29725918 29728407 29725918 ID=MS.gene76354.t1;Parent=MS.gene76354
chr8.1 exon 29725918 29726009 29725918 ID=MS.gene76354.t1.exon1;Parent=MS.gene76354.t1
chr8.1 CDS 29725918 29726009 29725918 ID=cds.MS.gene76354.t1;Parent=MS.gene76354.t1
chr8.1 exon 29726859 29726877 29726859 ID=MS.gene76354.t1.exon2;Parent=MS.gene76354.t1
chr8.1 CDS 29726859 29726877 29726859 ID=cds.MS.gene76354.t1;Parent=MS.gene76354.t1
chr8.1 exon 29727679 29727752 29727679 ID=MS.gene76354.t1.exon3;Parent=MS.gene76354.t1
chr8.1 CDS 29727679 29727752 29727679 ID=cds.MS.gene76354.t1;Parent=MS.gene76354.t1
chr8.1 exon 29728395 29728407 29728395 ID=MS.gene76354.t1.exon4;Parent=MS.gene76354.t1
chr8.1 CDS 29728395 29728407 29728395 ID=cds.MS.gene76354.t1;Parent=MS.gene76354.t1
Gene Sequence

>MS.gene76354

ATGGAACAGTGGGGAGAAGAAAAGATGGTCAACAAGTACCTCCAAGTGACAAGGTTTATGAATGCATTCTTTTCCGAGGAAGTGACATTAAGGTAACTATATTATCATTATTTAACTGGTTTGCAATTTATTGTTAATAAGAGAAATAAGCATATGATGAAATATTTCTGATTTCTTGCTTAGAAGCATCTCACCCCGCACAGGGGAGTTAATAAGGCTTGGCTGATGTTGTTGTATTGCTGTGATATATCTTAGTTTTGAGGTTTTAAAATAATATTATAATATATGAATGATTTCTGATTGAGACTTTAAAATTTGTTGTCCCTAGTTCGTTTATGCAACTTTTCACTAGTCATAATCTTTTACTCAATAGATTGATGTGTATGGTTTTCTTGTTAAGTATTGCTCAAATTCAGGTTCTTGGTGCAACTGATGTATTTTGGGGCCTTTGTATATTATTTTCCTTTTACTTCTACAAAATTCTCCTTTTTAGGTTGATCATATTCTTTTTAATAGGTGATTTTCTTTATAAAACTCTCACCAAGAAGGCTTAATCGACTGATTGGGCAAATATCTCTTTATAGACTTTTGCTAGATATATAATTTTACCACACAACTGATGAGATTGGATTATGTGTAGGTAGCTTGGAATGATGAACAAGAGGATCATTTATTTCTAGGAATGTTTGATGTTTTTTGTTCATTTAGTATAAAATTTCCCCTTTGCTATTATGAAATTTGAGGTGTATTATCATATTTTATTTATATTTATAGTTCATTTTATGTTACTATGATGATATTTTTAATGAACTTAGTACTGTTTTCTTATTTAGAAACCTTAAAGAATAGCAATTAACACCTTGTCTTTGTTTTCTGCAGCATCTTTACCTAATTGAAGTTTGCTATCTTTGGTTGATTTAATATCACTCCTTGTCATCAAGTAAACTTGTCTCTGAAAAGGTGAGATACTAGTTCTAAATTTTAATCCATGTTATTTATTTTTTTCGTAAGGTATTTTGATGGAGTTTATGTGTCTAACTGCCAATGGAATGTTGCAATGGAAATTCTGGATTTTTCTTATGATTGTTGTGTTCTAGCGTGGTAATCTTATCATAATAATTTTGTTTAGATTTCATACTAACTTAGTTGTTGATATGATAACATTTTAGTGGTTGGTGTTGACATATAGAAAGTTACTCTGCGATTCATGATTAGAAGAAAGATATATGGTGTATCAAGTAGCTCAAGTAGTCCAAACCAAGTATCCAATACACAAAACATGTTTCTGCTAATGTTTATAATTGAAGTTGATGATGTTTATAATTGAAGTTGATGATGTTTTTATGTTCATTTTCCTATACTTTTGATTTTTTAAAAATGAGTATTTTGTGAAAAATATTTTCTTGTTTTCTCTATTGAATTTACTGCTGTATATTTGTGCGGTCTGTTTTCTTGTCGTTTGTGGCCTTTGGTTCGGTTGAGGCTTGTTCAAAGTCCATTTGTTTTGCTGCTGTTCTGTTTTTTTGGCCTCTTTCGGGGCTCCGGAAAATTGGTTTCCGGTTAAATTTTTAGGGACGGTTGGTTTTTAGGTTCCTCAGGTATTTAAGCTATGTCGGTCTTGTATTTAAGAGCATGCAGCTGTGTTGAATACAATACTCAACAACTATTGATATGTGGTCTCTTTATTGTATAGTGAATGAATTAATTGATGATAACTATCTTGTCCATAAAACAACCATTTTTGTTTCTCAAATTTCTTAGGCATACAATGTTCTCAAAAGACCTGCTGATGTGATTGATAAATTTGACAGGAAAGCTCTAGAGCAATTAATCTGGTAAGAAATCTAGCTTTCTATTCATTTCCTCCATGCATTGCCCTCTACCATTTTTCGTTGAAGTATTTTATTAAGTGGTGGGTATAGATTATGATTTTGGTACTATTTAGATAGAATATGATGGTCTAGATAAACAATAGTGCTATGGGTTGAATTACTCGAGGTTGAGAATGTTGAAAATTATTTTAATGATAAGTTTGTTGATCTACTTGTAAATGTAACTTTCGGCAAGCAAATTCGAATCTGGGTTAGATACCAAGACTTTTAAGTTATTTTGATGAAATTTGAGTAGTCTAAGAATACAAAGGTAATGATATTATTGAGTTCCTTCACTAAATTTGGAGGGTTCAAATGCTGGTCTAGCATCCATACTGGAGTACCTGGAGTTTTGCTTGAACTCAAGACTGGTGTTTTGAATGAAAGGGTAATGATTTATTAGTTTCACATTGAATGCAATTAATTGGACTTGTAAGCTTCTCTGTAATAAATAGCTAATGGTTATTCCATAGTGGTTATTTTTGACAAATATATCAGAAAGTAATTATAAGTTTAATTGCGCGATAAGTTAGAATCATGACTTGCGTGACATTATTGCTTATTTTTGTTAATTGATGTGTCTTATATAATCTGACTTATTTTTAGGTGGAAGACTTAG
Protein sequence

>MS.gene76354.t1

MEQWGEEKMVNKYLQVTRFMNAFFSEEVTLSKLVSEKAYNVLKRPADVIDKFDRKALEQLIWWKT