AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76365


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76365.t1 MTR_5g036670 86.170 94 13 0 3 96 77 170 1.41e-47 166
MS.gene76365.t1 MTR_8g062700 71.250 80 23 0 3 82 76 155 7.45e-32 120
MS.gene76365.t1 MTR_8g062700 71.250 80 23 0 3 82 76 155 7.52e-32 120
MS.gene76365.t1 MTR_6g008970 64.773 88 31 0 3 90 97 184 1.22e-30 117
MS.gene76365.t1 MTR_6g008995 63.636 88 30 2 3 90 14 99 2.85e-27 106
MS.gene76365.t1 MTR_6g008995 63.636 88 30 2 3 90 71 156 4.46e-27 106
MS.gene76365.t1 MTR_1g023820 100.000 36 0 0 3 38 21 56 3.33e-19 79.3
MS.gene76365.t1 MTR_7g013410 41.935 93 43 3 3 92 32 116 9.87e-16 70.5
MS.gene76365.t1 MTR_8g062350 32.374 139 52 4 8 107 53 188 1.14e-13 66.6
MS.gene76365.t1 MTR_1g105955 44.872 78 36 2 1 78 48 118 1.18e-12 63.5
MS.gene76365.t1 MTR_0091s0080 34.066 91 56 2 89 179 387 473 4.33e-12 63.9
MS.gene76365.t1 MTR_1g105960 56.818 44 19 0 3 46 35 78 5.65e-12 60.1
MS.gene76365.t1 MTR_4g133300 36.905 84 47 1 3 86 43 120 3.57e-11 60.5
MS.gene76365.t1 MTR_4g133470 38.750 80 43 1 3 82 43 116 3.91e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76365.t1 AT1G70060 70.787 89 25 1 3 90 48 136 3.57e-34 127
MS.gene76365.t1 AT1G70060 70.787 89 25 1 3 90 48 136 3.57e-34 127
MS.gene76365.t1 AT1G24190 71.591 88 25 0 3 90 48 135 9.96e-34 126
MS.gene76365.t1 AT1G24190 71.591 88 25 0 3 90 48 135 1.02e-33 126
MS.gene76365.t1 AT1G24190 71.591 88 25 0 3 90 48 135 1.06e-33 126
MS.gene76365.t1 AT3G01320 60.000 90 35 1 3 92 91 179 1.02e-26 106
MS.gene76365.t1 AT5G15020 62.791 86 30 1 4 89 87 170 1.05e-26 106
MS.gene76365.t1 AT5G15020 62.791 86 30 1 4 89 87 170 1.05e-26 106
MS.gene76365.t1 AT3G01320 60.000 90 35 1 3 92 91 179 1.05e-26 106
MS.gene76365.t1 AT1G59890 59.091 88 34 1 3 90 79 164 2.61e-26 105
MS.gene76365.t1 AT1G59890 59.091 88 34 1 3 90 79 164 2.77e-26 105
MS.gene76365.t1 AT1G59890 59.091 88 34 1 3 90 79 164 2.87e-26 105
MS.gene76365.t1 AT1G59890 59.091 88 34 1 3 90 79 164 2.91e-26 105
MS.gene76365.t1 AT1G10450 61.250 80 29 1 3 82 67 144 1.43e-25 103
MS.gene76365.t1 AT1G10450 61.250 80 29 1 3 82 67 144 1.43e-25 103
MS.gene76365.t1 AT1G10450 61.250 80 29 1 3 82 118 195 1.46e-25 103
MS.gene76365.t1 AT1G24200 57.500 80 31 1 3 82 48 124 3.00e-22 89.0
MS.gene76365.t1 AT1G70030 53.947 76 33 2 8 83 50 123 1.11e-17 76.3
MS.gene76365.t1 AT5G35610 35.965 114 58 3 3 105 44 153 1.98e-13 65.1
MS.gene76365.t1 AT1G70030 50.000 72 33 2 8 77 61 131 1.89e-12 62.0
MS.gene76365.t1 AT1G70030 56.140 57 24 1 8 64 61 116 3.63e-12 60.8

Find 45 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGTTTACAAGTCATTTA+TGG 0.140196 8.1:-29829928 MS.gene76365:CDS
CTCATGGCCCTTTGGCAAAA+AGG 0.248205 8.1:+29830160 None:intergenic
CAAGAAGATCCGGGTGGTTT+TGG 0.249415 8.1:+29829725 None:intergenic
TTCATCTGTGTCGTGTTGAT+TGG 0.378078 8.1:-29829693 MS.gene76365:CDS
AAGTTAACCATTACTTTATG+AGG 0.408474 8.1:-29829491 MS.gene76365:CDS
TTGTATGAGTGCACTGCAAA+GGG 0.421632 8.1:+29829562 None:intergenic
TCGTGTTGATTGGCGGTGAC+TGG 0.441416 8.1:-29829683 MS.gene76365:CDS
GAGTTATGTGAACACGATAA+AGG 0.446913 8.1:-29830072 MS.gene76365:intron
AGGGTAATCTCATGGCCCTT+TGG 0.451315 8.1:+29830152 None:intergenic
ACTCGGCATCAGTGGGGAAG+GGG 0.451431 8.1:-29829613 MS.gene76365:CDS
ACTGCAAAGGGGTATGTCTC+AGG 0.456330 8.1:+29829574 None:intergenic
AACCACCCGGATCTTCTTGA+AGG 0.457397 8.1:-29829721 MS.gene76365:CDS
TGGTTGAGGTTGCTCATCCT+CGG 0.459938 8.1:+29830124 None:intergenic
CAGGTACTTTCCCTCTTGGT+AGG 0.460534 8.1:+29829465 None:intergenic
GGTCATAGAGACTTGATCTT+GGG 0.467943 8.1:-29830191 MS.gene76365:CDS
ACATCAGGTACTTTCCCTCT+TGG 0.471918 8.1:+29829461 None:intergenic
ATAAACCCTTCAAGAAGATC+CGG 0.477187 8.1:+29829715 None:intergenic
TTTATGCAGACTCGGTTTCA+GGG 0.487740 8.1:-29829957 MS.gene76365:intron
AAACTCGGCATCAGTGGGGA+AGG 0.492373 8.1:-29829615 MS.gene76365:CDS
CTATAAAACTCGGCATCAGT+GGG 0.502922 8.1:-29829620 MS.gene76365:CDS
AGGTACTTTCCCTCTTGGTA+GGG 0.505190 8.1:+29829466 None:intergenic
GCTATAAAACTCGGCATCAG+TGG 0.506265 8.1:-29829621 MS.gene76365:CDS
ATCTGTGTCGTGTTGATTGG+CGG 0.509936 8.1:-29829690 MS.gene76365:CDS
AGGTCATAGAGACTTGATCT+TGG 0.513611 8.1:-29830192 MS.gene76365:CDS
CTCGGCATCAGTGGGGAAGG+GGG 0.515169 8.1:-29829612 MS.gene76365:CDS
AACTCGGCATCAGTGGGGAA+GGG 0.528339 8.1:-29829614 MS.gene76365:CDS
TTGCTCATCCTCGGACGAAA+GGG 0.539598 8.1:+29830133 None:intergenic
CTTGTATGAGTGCACTGCAA+AGG 0.547650 8.1:+29829561 None:intergenic
GTTGCTCATCCTCGGACGAA+AGG 0.549267 8.1:+29830132 None:intergenic
ACCACCCGGATCTTCTTGAA+GGG 0.554045 8.1:-29829720 MS.gene76365:CDS
TATATTCATCCTTCGAGCAA+TGG 0.563072 8.1:-29829537 MS.gene76365:CDS
CGGACGAAAGGGTAATCTCA+TGG 0.581909 8.1:+29830144 None:intergenic
TGAGATTACCCTTTCGTCCG+AGG 0.584557 8.1:-29830141 MS.gene76365:CDS
TAAACCCTTCAAGAAGATCC+GGG 0.599628 8.1:+29829716 None:intergenic
TGCATTCTTCCAAAACCACC+CGG 0.599692 8.1:-29829734 MS.gene76365:CDS
GTTGGTCATGCTATAAAACT+CGG 0.605058 8.1:-29829630 MS.gene76365:CDS
TCATTGACACCATTGCTCGA+AGG 0.620805 8.1:+29829528 None:intergenic
TAGGGCACCTCATAAAGTAA+TGG 0.626853 8.1:+29829484 None:intergenic
ATTTGTAGAGTTGATACTGA+TGG 0.633224 8.1:-29830245 MS.gene76365:intron
TATGAGGTGCCCTACCAAGA+GGG 0.635144 8.1:-29829475 MS.gene76365:CDS
TTATGAGGTGCCCTACCAAG+AGG 0.660657 8.1:-29829476 MS.gene76365:CDS
TGGTGTCATTGAAAGAGTGA+AGG 0.685356 8.1:-29830225 MS.gene76365:CDS
TGTATGAGTGCACTGCAAAG+GGG 0.718580 8.1:+29829563 None:intergenic
ACCCTTCAAGAAGATCCGGG+TGG 0.747614 8.1:+29829719 None:intergenic
TATAAAACTCGGCATCAGTG+GGG 0.747744 8.1:-29829619 MS.gene76365:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTGTTTTTGTATATATATT+GGG - chr8.1:29829476-29829495 MS.gene76365:CDS 15.0%
!! ATATATATACAAAAACAACG+TGG + chr8.1:29829475-29829494 None:intergenic 20.0%
!!! AATTGATTTGTTTATCCTTT+TGG - chr8.1:29829525-29829544 MS.gene76365:CDS 20.0%
!!! CGTTGTTTTTGTATATATAT+TGG - chr8.1:29829475-29829494 MS.gene76365:CDS 20.0%
!!! TAAAATTCAACCTTCTTTTT+TGG + chr8.1:29829695-29829714 None:intergenic 20.0%
! AAGTTAACCATTACTTTATG+AGG - chr8.1:29830305-29830324 MS.gene76365:intron 25.0%
! TAGAATGTTTGTGAGTTTAA+TGG - chr8.1:29829748-29829767 MS.gene76365:intron 25.0%
! TCATGTTTACAAGTCATTTA+TGG - chr8.1:29829868-29829887 MS.gene76365:intron 25.0%
! TTTGTATATATATTGGGAGA+GGG - chr8.1:29829482-29829501 MS.gene76365:CDS 25.0%
!! TTTTGTATATATATTGGGAG+AGG - chr8.1:29829481-29829500 MS.gene76365:CDS 25.0%
!!! AGTTTTAGTTTTGAGCTTAA+TGG - chr8.1:29829810-29829829 MS.gene76365:intron 25.0%
CATATTGAATACGTACAGAA+AGG - chr8.1:29829892-29829911 MS.gene76365:intron 30.0%
! ATTTAACACCTTTTTGCCAA+AGG - chr8.1:29829628-29829647 MS.gene76365:CDS 30.0%
! TTTAACACCTTTTTGCCAAA+GGG - chr8.1:29829629-29829648 MS.gene76365:CDS 30.0%
!! ATTTGTAGAGTTGATACTGA+TGG - chr8.1:29829551-29829570 MS.gene76365:CDS 30.0%
AACTCTACAAATGCACCAAA+AGG + chr8.1:29829543-29829562 None:intergenic 35.0%
ATAAACCCTTCAAGAAGATC+CGG + chr8.1:29830084-29830103 None:intergenic 35.0%
GAGTTATGTGAACACGATAA+AGG - chr8.1:29829724-29829743 MS.gene76365:CDS 35.0%
GTCAGGAAAATCTGTAATGT+TGG - chr8.1:29829962-29829981 MS.gene76365:intron 35.0%
GTTGGTCATGCTATAAAACT+CGG - chr8.1:29830166-29830185 MS.gene76365:CDS 35.0%
TATATTCATCCTTCGAGCAA+TGG - chr8.1:29830259-29830278 MS.gene76365:intron 35.0%
! AGAGTGAAGGAACTTTTTAG+AGG - chr8.1:29829584-29829603 MS.gene76365:CDS 35.0%
!!! TCAACCTTCTTTTTTGGTTG+AGG + chr8.1:29829689-29829708 None:intergenic 35.0%
AATCATGATTGCACTTCTCC+AGG - chr8.1:29830035-29830054 MS.gene76365:intron 40.0%
ACATACCTCTTTGTAGACCA+GGG + chr8.1:29829933-29829952 None:intergenic 40.0%
AGGTCATAGAGACTTGATCT+TGG - chr8.1:29829604-29829623 MS.gene76365:CDS 40.0%
CACAGCAATATGTCAAGTAC+TGG + chr8.1:29830004-29830023 None:intergenic 40.0%
CTATAAAACTCGGCATCAGT+GGG - chr8.1:29830176-29830195 MS.gene76365:CDS 40.0%
GAAACCGAGTCTGCATAAAA+AGG + chr8.1:29829838-29829857 None:intergenic 40.0%
GCAACCTCAACCAAAAAAGA+AGG - chr8.1:29829682-29829701 MS.gene76365:CDS 40.0%
GGTCATAGAGACTTGATCTT+GGG - chr8.1:29829605-29829624 MS.gene76365:CDS 40.0%
TAAACCCTTCAAGAAGATCC+GGG + chr8.1:29830083-29830102 None:intergenic 40.0%
TATAAAACTCGGCATCAGTG+GGG - chr8.1:29830177-29830196 MS.gene76365:CDS 40.0%
TTGTATGAGTGCACTGCAAA+GGG + chr8.1:29830237-29830256 None:intergenic 40.0%
! AGGTATGTGCTGTTATAGTC+AGG - chr8.1:29829945-29829964 MS.gene76365:CDS 40.0%
! GGTTCCTTTTTATGCAGACT+CGG - chr8.1:29829831-29829850 MS.gene76365:intron 40.0%
! GTGTTGCGTAATTTTCGTGT+TGG - chr8.1:29830148-29830167 MS.gene76365:CDS 40.0%
! TAGGGCACCTCATAAAGTAA+TGG + chr8.1:29830315-29830334 None:intergenic 40.0%
! TTCATCTGTGTCGTGTTGAT+TGG - chr8.1:29830103-29830122 MS.gene76365:CDS 40.0%
! TTTATGCAGACTCGGTTTCA+GGG - chr8.1:29829839-29829858 MS.gene76365:intron 40.0%
!! AGCAATATGTCAAGTACTGG+AGG + chr8.1:29830001-29830020 None:intergenic 40.0%
!! TGGTGTCATTGAAAGAGTGA+AGG - chr8.1:29829571-29829590 MS.gene76365:CDS 40.0%
!! TTTTATGCAGACTCGGTTTC+AGG - chr8.1:29829838-29829857 MS.gene76365:intron 40.0%
AGGTACTTTCCCTCTTGGTA+GGG + chr8.1:29830333-29830352 None:intergenic 45.0%
CACATACCTCTTTGTAGACC+AGG + chr8.1:29829934-29829953 None:intergenic 45.0%
CTTGTATGAGTGCACTGCAA+AGG + chr8.1:29830238-29830257 None:intergenic 45.0%
GCTATAAAACTCGGCATCAG+TGG - chr8.1:29830175-29830194 MS.gene76365:CDS 45.0%
TATCACCCTGGTCTACAAAG+AGG - chr8.1:29829925-29829944 MS.gene76365:CDS 45.0%
TCATTGACACCATTGCTCGA+AGG + chr8.1:29830271-29830290 None:intergenic 45.0%
TCTTTGTAGACCAGGGTGAT+AGG + chr8.1:29829926-29829945 None:intergenic 45.0%
TGCATTCTTCCAAAACCACC+CGG - chr8.1:29830062-29830081 MS.gene76365:intron 45.0%
TGTATGAGTGCACTGCAAAG+GGG + chr8.1:29830236-29830255 None:intergenic 45.0%
TTTGGAAGAATGCAGCAACC+TGG + chr8.1:29830056-29830075 None:intergenic 45.0%
!! ATCTGTGTCGTGTTGATTGG+CGG - chr8.1:29830106-29830125 MS.gene76365:CDS 45.0%
AACCACCCGGATCTTCTTGA+AGG - chr8.1:29830075-29830094 MS.gene76365:CDS 50.0%
ACCACCCGGATCTTCTTGAA+GGG - chr8.1:29830076-29830095 MS.gene76365:CDS 50.0%
AGGGTAATCTCATGGCCCTT+TGG + chr8.1:29829647-29829666 None:intergenic 50.0%
CAGGTACTTTCCCTCTTGGT+AGG + chr8.1:29830334-29830353 None:intergenic 50.0%
CGGACGAAAGGGTAATCTCA+TGG + chr8.1:29829655-29829674 None:intergenic 50.0%
CTCATGGCCCTTTGGCAAAA+AGG + chr8.1:29829639-29829658 None:intergenic 50.0%
GGAAAGTAAGCCTATCACCC+TGG - chr8.1:29829913-29829932 MS.gene76365:intron 50.0%
TGAGATTACCCTTTCGTCCG+AGG - chr8.1:29829655-29829674 MS.gene76365:CDS 50.0%
TGGTTGAGGTTGCTCATCCT+CGG + chr8.1:29829675-29829694 None:intergenic 50.0%
TTGCTCATCCTCGGACGAAA+GGG + chr8.1:29829666-29829685 None:intergenic 50.0%
! ACTGCAAAGGGGTATGTCTC+AGG + chr8.1:29830225-29830244 None:intergenic 50.0%
! CAAGAAGATCCGGGTGGTTT+TGG + chr8.1:29830074-29830093 None:intergenic 50.0%
! TATGAGGTGCCCTACCAAGA+GGG - chr8.1:29830321-29830340 MS.gene76365:intron 50.0%
! TTATGAGGTGCCCTACCAAG+AGG - chr8.1:29830320-29830339 MS.gene76365:intron 50.0%
AAACTCGGCATCAGTGGGGA+AGG - chr8.1:29830181-29830200 MS.gene76365:CDS 55.0%
AACTCGGCATCAGTGGGGAA+GGG - chr8.1:29830182-29830201 MS.gene76365:CDS 55.0%
ACCCTTCAAGAAGATCCGGG+TGG + chr8.1:29830080-29830099 None:intergenic 55.0%
GTTGCTCATCCTCGGACGAA+AGG + chr8.1:29829667-29829686 None:intergenic 55.0%
!! TCGTGTTGATTGGCGGTGAC+TGG - chr8.1:29830113-29830132 MS.gene76365:CDS 55.0%
ACTCGGCATCAGTGGGGAAG+GGG - chr8.1:29830183-29830202 MS.gene76365:CDS 60.0%
CTCGGCATCAGTGGGGAAGG+GGG - chr8.1:29830184-29830203 MS.gene76365:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 29829464 29830354 29829464 ID=MS.gene76365
chr8.1 mRNA 29829464 29830354 29829464 ID=MS.gene76365.t1;Parent=MS.gene76365
chr8.1 exon 29830350 29830354 29830350 ID=MS.gene76365.t1.exon1;Parent=MS.gene76365.t1
chr8.1 CDS 29830350 29830354 29830350 ID=cds.MS.gene76365.t1;Parent=MS.gene76365.t1
chr8.1 exon 29830073 29830259 29830073 ID=MS.gene76365.t1.exon2;Parent=MS.gene76365.t1
chr8.1 CDS 29830073 29830259 29830073 ID=cds.MS.gene76365.t1;Parent=MS.gene76365.t1
chr8.1 exon 29829915 29829970 29829915 ID=MS.gene76365.t1.exon3;Parent=MS.gene76365.t1
chr8.1 CDS 29829915 29829970 29829915 ID=cds.MS.gene76365.t1;Parent=MS.gene76365.t1
chr8.1 exon 29829464 29829761 29829464 ID=MS.gene76365.t1.exon4;Parent=MS.gene76365.t1
chr8.1 CDS 29829464 29829761 29829464 ID=cds.MS.gene76365.t1;Parent=MS.gene76365.t1
Gene Sequence

>MS.gene76365

ATGTTGTACCACGTTGTTTTTGTATATATATTGGGAGAGGGATTATGAATTTGTTAGTTGTAATTGATTTGTTTATCCTTTTGGTGCATTTGTAGAGTTGATACTGATGGTGTCATTGAAAGAGTGAAGGAACTTTTTAGAGGTCATAGAGACTTGATCTTGGGATTTAACACCTTTTTGCCAAAGGGCCATGAGATTACCCTTTCGTCCGAGGATGAGCAACCTCAACCAAAAAAGAAGGTTGAATTTTATGAAGCTACGAGTTATGTGAACACGATAAAGGTTAGAATGTTTGTGAGTTTAATGGATTCTCTTCATGCAGATTTGTTTGCAATGTAGAATTAATAGTTTTAGTTTTGAGCTTAATGGTTCCTTTTTATGCAGACTCGGTTTCAGGGCGATGTTCATGTTTACAAGTCATTTATGGACATATTGAATACGTACAGAAAGGAAAGTAAGCCTATCACCCTGGTCTACAAAGAGGTATGTGCTGTTATAGTCAGGAAAATCTGTAATGTTGGTTTTTTTTTATTTCCTCCAGTACTTGACATATTGCTGTGATACACATTTTAATCATGATTGCACTTCTCCAGGTTGCTGCATTCTTCCAAAACCACCCGGATCTTCTTGAAGGGTTTATTCATCTGTGTCGTGTTGATTGGCGGTGACTGGAAAGCTATCTGTGTGTTGCGTAATTTTCGTGTTGGTCATGCTATAAAACTCGGCATCAGTGGGGAAGGGGGTGCTGATGTTGTGTACCTGAGACATACCCCTTTGCAGTGCACTCATACAAGCTATATTCATCCTTCGAGCAATGGTGTCAATGAGAAATTTGTATATCAAGTTAACCATTACTTTATGAGGTGCCCTACCAAGAGGGAAAGTACCTGA

Protein sequence

>MS.gene76365.t1

MLVDTDGVIERVKELFRGHRDLILGFNTFLPKGHEITLSSEDEQPQPKKKVEFYEATSYVNTIKTRFQGDVHVYKSFMDILNTLLHSSKTTRIFLKGLFICVVLIGGDWKAICVLRNFRVGHAIKLGISGEGGADVVYLRHTPLQCTHTSYIHPSSNGVNEKFVYQVNHYFMRCPTKREST