AlfalfaGEDB | MS.gene76368

M. sativa cultivar XinJiangDaYe / MS.gene76368

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76368.t1	XP_003613438.2	86.2	94	13	0	3	96	77	170	6.10E-37	164.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76368.t1	O48686	71.6	88	25	0	3	90	48	135	4.4e-28	125.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76368.t1	G7K6W4	86.2	94	13	0	3	96	77	170	4.4e-37	164.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene76368.t1	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051262	MS.gene76368	0.855145	7.68E-62	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76368.t1	MTR_5g036670	86.170	94	13	0	3	96	77	170	1.41e-47	166
MS.gene76368.t1	MTR_8g062700	71.250	80	23	0	3	82	76	155	7.45e-32	120
MS.gene76368.t1	MTR_8g062700	71.250	80	23	0	3	82	76	155	7.52e-32	120
MS.gene76368.t1	MTR_6g008970	64.773	88	31	0	3	90	97	184	1.22e-30	117
MS.gene76368.t1	MTR_6g008995	63.636	88	30	2	3	90	14	99	2.85e-27	106
MS.gene76368.t1	MTR_6g008995	63.636	88	30	2	3	90	71	156	4.46e-27	106
MS.gene76368.t1	MTR_1g023820	100.000	36	0	0	3	38	21	56	3.33e-19	79.3
MS.gene76368.t1	MTR_7g013410	41.935	93	43	3	3	92	32	116	9.87e-16	70.5
MS.gene76368.t1	MTR_8g062350	32.374	139	52	4	8	107	53	188	1.14e-13	66.6
MS.gene76368.t1	MTR_1g105955	44.872	78	36	2	1	78	48	118	1.18e-12	63.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76368.t1	AT1G70060	70.787	89	25	1	3	90	48	136	3.57e-34	127
MS.gene76368.t1	AT1G70060	70.787	89	25	1	3	90	48	136	3.57e-34	127
MS.gene76368.t1	AT1G24190	71.591	88	25	0	3	90	48	135	9.96e-34	126
MS.gene76368.t1	AT1G24190	71.591	88	25	0	3	90	48	135	1.02e-33	126
MS.gene76368.t1	AT1G24190	71.591	88	25	0	3	90	48	135	1.06e-33	126
MS.gene76368.t1	AT3G01320	60.000	90	35	1	3	92	91	179	1.02e-26	106
MS.gene76368.t1	AT5G15020	62.791	86	30	1	4	89	87	170	1.05e-26	106
MS.gene76368.t1	AT5G15020	62.791	86	30	1	4	89	87	170	1.05e-26	106
MS.gene76368.t1	AT3G01320	60.000	90	35	1	3	92	91	179	1.05e-26	106
MS.gene76368.t1	AT1G59890	59.091	88	34	1	3	90	79	164	2.61e-26	105

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Find 45 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCGGCATCAGTGGGGA+AGG	0.492373	8.1:+29853251	MS.gene76368:CDS
AACCACCCGGATCTTCTTGA+AGG	0.457397	8.1:+29853145	MS.gene76368:CDS
AACTCGGCATCAGTGGGGAA+GGG	0.528339	8.1:+29853252	MS.gene76368:CDS
AAGTTAACCATTACTTTATG+AGG	0.408474	8.1:+29853375	MS.gene76368:CDS
ACATCAGGTACTTTCCCTCT+TGG	0.471918	8.1:-29853405	None:intergenic
ACCACCCGGATCTTCTTGAA+GGG	0.554045	8.1:+29853146	MS.gene76368:CDS
ACCCTTCAAGAAGATCCGGG+TGG	0.747614	8.1:-29853147	None:intergenic
ACTCGGCATCAGTGGGGAAG+GGG	0.451431	8.1:+29853253	MS.gene76368:CDS
ACTGCAAAGGGGTATGTCTC+AGG	0.456330	8.1:-29853292	None:intergenic
AGGGTAATCTCATGGCCCTT+TGG	0.451315	8.1:-29852713	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATATTGAATACGTACAGAA+AGG	+	chr8.1:29852961-29852980	MS.gene76368:intron	30.0%
AACTCTACAAATGCACCAAA+AGG	-	chr8.1:29852612-29852631	None:intergenic	35.0%
ATAAACCCTTCAAGAAGATC+CGG	-	chr8.1:29853154-29853173	None:intergenic	35.0%
GAGTTATGTGAACACGATAA+AGG	+	chr8.1:29852793-29852812	MS.gene76368:CDS	35.0%
GTCAGGAAAATCTGTAATGT+TGG	+	chr8.1:29853031-29853050	MS.gene76368:intron	35.0%
GTTGGTCATGCTATAAAACT+CGG	+	chr8.1:29853236-29853255	MS.gene76368:CDS	35.0%
TATATTCATCCTTCGAGCAA+TGG	+	chr8.1:29853329-29853348	MS.gene76368:CDS	35.0%
AATCATGATTGCACTTCTCC+AGG	+	chr8.1:29853105-29853124	MS.gene76368:intron	40.0%
ACATACCTCTTTGTAGACCA+GGG	-	chr8.1:29853002-29853021	None:intergenic	40.0%
AGGTCATAGAGACTTGATCT+TGG	+	chr8.1:29852673-29852692	MS.gene76368:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	29852533	29853424	29852533	ID=MS.gene76368
chr8.1	mRNA	29852533	29853424	29852533	ID=MS.gene76368.t1;Parent=MS.gene76368
chr8.1	exon	29852533	29852537	29852533	ID=MS.gene76368.t1.exon1;Parent=MS.gene76368.t1
chr8.1	CDS	29852533	29852537	29852533	ID=cds.MS.gene76368.t1;Parent=MS.gene76368.t1
chr8.1	exon	29852628	29852814	29852628	ID=MS.gene76368.t1.exon2;Parent=MS.gene76368.t1
chr8.1	CDS	29852628	29852814	29852628	ID=cds.MS.gene76368.t1;Parent=MS.gene76368.t1
chr8.1	exon	29852917	29852972	29852917	ID=MS.gene76368.t1.exon3;Parent=MS.gene76368.t1
chr8.1	CDS	29852917	29852972	29852917	ID=cds.MS.gene76368.t1;Parent=MS.gene76368.t1
chr8.1	exon	29853127	29853424	29853127	ID=MS.gene76368.t1.exon4;Parent=MS.gene76368.t1
chr8.1	CDS	29853127	29853424	29853127	ID=cds.MS.gene76368.t1;Parent=MS.gene76368.t1

Gene Sequence

>MS.gene76368

ATGTTGTACCACGTTGTTTTTGTATATATATTGGGAGAGGGATTATGAATTTGTTAGTTGTAATTGATTTGTTTATCCTTTTGGTGCATTTGTAGAGTTGATACTGATGGTGTCATTGAAAGAGTGAAGGAACTTTTTAGAGGTCATAGAGACTTGATCTTGGGATTTAACACCTTTTTGCCAAAGGGCCATGAGATTACCCTTTCGTCCGAGGATGAGCAACCTCAACCAAAAAAGAAGGTTGAATTTTATGAAGCTACGAGTTATGTGAACACGATAAAGGTTAGAATGTTTGTGAGTTTAATGGATTCTCTTCATGCAGATTTGTTTGCAATGTAGAATTAATAGTTTTAGTTTTGAGCTTAATGGTTCCTTTTTATGCAGACTCGGTTTCAGGGCGATGTTCATGTTTACAAGTCATTTATGGACATATTGAATACGTACAGAAAGGAAAGTAAGCCTATCACCCTGGTCTACAAAGAGGTATGTGCTGTTATAGTCAGGAAAATCTGTAATGTTGGTTTTTTTTTTATTTCCTCCAGTACTTGACATATTGCTGTGATACACATTTTAATCATGATTGCACTTCTCCAGGTTGCTGCATTCTTCCAAAACCACCCGGATCTTCTTGAAGGGTTTATTCATCTGTGTCGTGTTGATTGGCGGTGACTGGAAAGCTATCTGTGTGTTGCGTAATTTTCGTGTTGGTCATGCTATAAAACTCGGCATCAGTGGGGAAGGGGGTGCTGATGTTGTGTACCTGAGACATACCCCTTTGCAGTGCACTCATACAAGCTATATTCATCCTTCGAGCAATGGTGTCAATGAGAAATTTGTATATCAAGTTAACCATTACTTTATGAGGTGCCCTACCAAGAGGGAAAGTACCTGA

Protein sequence

>MS.gene76368.t1

MLVDTDGVIERVKELFRGHRDLILGFNTFLPKGHEITLSSEDEQPQPKKKVEFYEATSYVNTIKTRFQGDVHVYKSFMDILNTLLHSSKTTRIFLKGLFICVVLIGGDWKAICVLRNFRVGHAIKLGISGEGGADVVYLRHTPLQCTHTSYIHPSSNGVNEKFVYQVNHYFMRCPTKREST