AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76368


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76368.t1 MTR_5g036670 86.170 94 13 0 3 96 77 170 1.41e-47 166
MS.gene76368.t1 MTR_8g062700 71.250 80 23 0 3 82 76 155 7.45e-32 120
MS.gene76368.t1 MTR_8g062700 71.250 80 23 0 3 82 76 155 7.52e-32 120
MS.gene76368.t1 MTR_6g008970 64.773 88 31 0 3 90 97 184 1.22e-30 117
MS.gene76368.t1 MTR_6g008995 63.636 88 30 2 3 90 14 99 2.85e-27 106
MS.gene76368.t1 MTR_6g008995 63.636 88 30 2 3 90 71 156 4.46e-27 106
MS.gene76368.t1 MTR_1g023820 100.000 36 0 0 3 38 21 56 3.33e-19 79.3
MS.gene76368.t1 MTR_7g013410 41.935 93 43 3 3 92 32 116 9.87e-16 70.5
MS.gene76368.t1 MTR_8g062350 32.374 139 52 4 8 107 53 188 1.14e-13 66.6
MS.gene76368.t1 MTR_1g105955 44.872 78 36 2 1 78 48 118 1.18e-12 63.5
MS.gene76368.t1 MTR_0091s0080 34.066 91 56 2 89 179 387 473 4.33e-12 63.9
MS.gene76368.t1 MTR_1g105960 56.818 44 19 0 3 46 35 78 5.65e-12 60.1
MS.gene76368.t1 MTR_4g133300 36.905 84 47 1 3 86 43 120 3.57e-11 60.5
MS.gene76368.t1 MTR_4g133470 38.750 80 43 1 3 82 43 116 3.91e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76368.t1 AT1G70060 70.787 89 25 1 3 90 48 136 3.57e-34 127
MS.gene76368.t1 AT1G70060 70.787 89 25 1 3 90 48 136 3.57e-34 127
MS.gene76368.t1 AT1G24190 71.591 88 25 0 3 90 48 135 9.96e-34 126
MS.gene76368.t1 AT1G24190 71.591 88 25 0 3 90 48 135 1.02e-33 126
MS.gene76368.t1 AT1G24190 71.591 88 25 0 3 90 48 135 1.06e-33 126
MS.gene76368.t1 AT3G01320 60.000 90 35 1 3 92 91 179 1.02e-26 106
MS.gene76368.t1 AT5G15020 62.791 86 30 1 4 89 87 170 1.05e-26 106
MS.gene76368.t1 AT5G15020 62.791 86 30 1 4 89 87 170 1.05e-26 106
MS.gene76368.t1 AT3G01320 60.000 90 35 1 3 92 91 179 1.05e-26 106
MS.gene76368.t1 AT1G59890 59.091 88 34 1 3 90 79 164 2.61e-26 105
MS.gene76368.t1 AT1G59890 59.091 88 34 1 3 90 79 164 2.77e-26 105
MS.gene76368.t1 AT1G59890 59.091 88 34 1 3 90 79 164 2.87e-26 105
MS.gene76368.t1 AT1G59890 59.091 88 34 1 3 90 79 164 2.91e-26 105
MS.gene76368.t1 AT1G10450 61.250 80 29 1 3 82 67 144 1.43e-25 103
MS.gene76368.t1 AT1G10450 61.250 80 29 1 3 82 67 144 1.43e-25 103
MS.gene76368.t1 AT1G10450 61.250 80 29 1 3 82 118 195 1.46e-25 103
MS.gene76368.t1 AT1G24200 57.500 80 31 1 3 82 48 124 3.00e-22 89.0
MS.gene76368.t1 AT1G70030 53.947 76 33 2 8 83 50 123 1.11e-17 76.3
MS.gene76368.t1 AT5G35610 35.965 114 58 3 3 105 44 153 1.98e-13 65.1
MS.gene76368.t1 AT1G70030 50.000 72 33 2 8 77 61 131 1.89e-12 62.0
MS.gene76368.t1 AT1G70030 56.140 57 24 1 8 64 61 116 3.63e-12 60.8

Find 45 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGTTTACAAGTCATTTA+TGG 0.140196 8.1:+29852937 MS.gene76368:CDS
CTCATGGCCCTTTGGCAAAA+AGG 0.248205 8.1:-29852705 None:intergenic
CAAGAAGATCCGGGTGGTTT+TGG 0.249415 8.1:-29853141 None:intergenic
TTCATCTGTGTCGTGTTGAT+TGG 0.378078 8.1:+29853173 MS.gene76368:CDS
AAGTTAACCATTACTTTATG+AGG 0.408474 8.1:+29853375 MS.gene76368:CDS
TTGTATGAGTGCACTGCAAA+GGG 0.421632 8.1:-29853304 None:intergenic
TCGTGTTGATTGGCGGTGAC+TGG 0.441416 8.1:+29853183 MS.gene76368:CDS
GAGTTATGTGAACACGATAA+AGG 0.446913 8.1:+29852793 MS.gene76368:CDS
AGGGTAATCTCATGGCCCTT+TGG 0.451315 8.1:-29852713 None:intergenic
ACTCGGCATCAGTGGGGAAG+GGG 0.451431 8.1:+29853253 MS.gene76368:CDS
ACTGCAAAGGGGTATGTCTC+AGG 0.456330 8.1:-29853292 None:intergenic
AACCACCCGGATCTTCTTGA+AGG 0.457397 8.1:+29853145 MS.gene76368:CDS
TGGTTGAGGTTGCTCATCCT+CGG 0.459938 8.1:-29852741 None:intergenic
CAGGTACTTTCCCTCTTGGT+AGG 0.460534 8.1:-29853401 None:intergenic
GGTCATAGAGACTTGATCTT+GGG 0.467943 8.1:+29852674 MS.gene76368:CDS
ACATCAGGTACTTTCCCTCT+TGG 0.471918 8.1:-29853405 None:intergenic
ATAAACCCTTCAAGAAGATC+CGG 0.477187 8.1:-29853151 None:intergenic
TTTATGCAGACTCGGTTTCA+GGG 0.487740 8.1:+29852908 MS.gene76368:intron
AAACTCGGCATCAGTGGGGA+AGG 0.492373 8.1:+29853251 MS.gene76368:CDS
CTATAAAACTCGGCATCAGT+GGG 0.502922 8.1:+29853246 MS.gene76368:CDS
AGGTACTTTCCCTCTTGGTA+GGG 0.505190 8.1:-29853400 None:intergenic
GCTATAAAACTCGGCATCAG+TGG 0.506265 8.1:+29853245 MS.gene76368:CDS
ATCTGTGTCGTGTTGATTGG+CGG 0.509936 8.1:+29853176 MS.gene76368:CDS
AGGTCATAGAGACTTGATCT+TGG 0.513611 8.1:+29852673 MS.gene76368:CDS
CTCGGCATCAGTGGGGAAGG+GGG 0.515169 8.1:+29853254 MS.gene76368:CDS
AACTCGGCATCAGTGGGGAA+GGG 0.528339 8.1:+29853252 MS.gene76368:CDS
TTGCTCATCCTCGGACGAAA+GGG 0.539598 8.1:-29852732 None:intergenic
CTTGTATGAGTGCACTGCAA+AGG 0.547650 8.1:-29853305 None:intergenic
GTTGCTCATCCTCGGACGAA+AGG 0.549267 8.1:-29852733 None:intergenic
ACCACCCGGATCTTCTTGAA+GGG 0.554045 8.1:+29853146 MS.gene76368:CDS
TATATTCATCCTTCGAGCAA+TGG 0.563072 8.1:+29853329 MS.gene76368:CDS
CGGACGAAAGGGTAATCTCA+TGG 0.581909 8.1:-29852721 None:intergenic
TGAGATTACCCTTTCGTCCG+AGG 0.584557 8.1:+29852724 MS.gene76368:CDS
TAAACCCTTCAAGAAGATCC+GGG 0.599628 8.1:-29853150 None:intergenic
TGCATTCTTCCAAAACCACC+CGG 0.599692 8.1:+29853132 MS.gene76368:CDS
GTTGGTCATGCTATAAAACT+CGG 0.605058 8.1:+29853236 MS.gene76368:CDS
TCATTGACACCATTGCTCGA+AGG 0.620805 8.1:-29853338 None:intergenic
TAGGGCACCTCATAAAGTAA+TGG 0.626853 8.1:-29853382 None:intergenic
ATTTGTAGAGTTGATACTGA+TGG 0.633224 8.1:+29852620 MS.gene76368:intron
TATGAGGTGCCCTACCAAGA+GGG 0.635144 8.1:+29853391 MS.gene76368:CDS
TTATGAGGTGCCCTACCAAG+AGG 0.660657 8.1:+29853390 MS.gene76368:CDS
TGGTGTCATTGAAAGAGTGA+AGG 0.685356 8.1:+29852640 MS.gene76368:CDS
TGTATGAGTGCACTGCAAAG+GGG 0.718580 8.1:-29853303 None:intergenic
ACCCTTCAAGAAGATCCGGG+TGG 0.747614 8.1:-29853147 None:intergenic
TATAAAACTCGGCATCAGTG+GGG 0.747744 8.1:+29853247 MS.gene76368:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTGTTTTTGTATATATATT+GGG + chr8.1:29852545-29852564 MS.gene76368:intron 15.0%
!! ATATATATACAAAAACAACG+TGG - chr8.1:29852544-29852563 None:intergenic 20.0%
!!! AATTGATTTGTTTATCCTTT+TGG + chr8.1:29852594-29852613 MS.gene76368:intron 20.0%
!!! CGTTGTTTTTGTATATATAT+TGG + chr8.1:29852544-29852563 MS.gene76368:intron 20.0%
!!! TAAAATTCAACCTTCTTTTT+TGG - chr8.1:29852764-29852783 None:intergenic 20.0%
! AAGTTAACCATTACTTTATG+AGG + chr8.1:29853375-29853394 MS.gene76368:CDS 25.0%
! TAGAATGTTTGTGAGTTTAA+TGG + chr8.1:29852817-29852836 MS.gene76368:intron 25.0%
! TCATGTTTACAAGTCATTTA+TGG + chr8.1:29852937-29852956 MS.gene76368:CDS 25.0%
! TTTGTATATATATTGGGAGA+GGG + chr8.1:29852551-29852570 MS.gene76368:intron 25.0%
!! TTTTGTATATATATTGGGAG+AGG + chr8.1:29852550-29852569 MS.gene76368:intron 25.0%
!!! AGTTTTAGTTTTGAGCTTAA+TGG + chr8.1:29852879-29852898 MS.gene76368:intron 25.0%
CATATTGAATACGTACAGAA+AGG + chr8.1:29852961-29852980 MS.gene76368:intron 30.0%
! ATTTAACACCTTTTTGCCAA+AGG + chr8.1:29852697-29852716 MS.gene76368:CDS 30.0%
! TTTAACACCTTTTTGCCAAA+GGG + chr8.1:29852698-29852717 MS.gene76368:CDS 30.0%
!! ATTTGTAGAGTTGATACTGA+TGG + chr8.1:29852620-29852639 MS.gene76368:intron 30.0%
AACTCTACAAATGCACCAAA+AGG - chr8.1:29852612-29852631 None:intergenic 35.0%
ATAAACCCTTCAAGAAGATC+CGG - chr8.1:29853154-29853173 None:intergenic 35.0%
GAGTTATGTGAACACGATAA+AGG + chr8.1:29852793-29852812 MS.gene76368:CDS 35.0%
GTCAGGAAAATCTGTAATGT+TGG + chr8.1:29853031-29853050 MS.gene76368:intron 35.0%
GTTGGTCATGCTATAAAACT+CGG + chr8.1:29853236-29853255 MS.gene76368:CDS 35.0%
TATATTCATCCTTCGAGCAA+TGG + chr8.1:29853329-29853348 MS.gene76368:CDS 35.0%
! AGAGTGAAGGAACTTTTTAG+AGG + chr8.1:29852653-29852672 MS.gene76368:CDS 35.0%
!!! TCAACCTTCTTTTTTGGTTG+AGG - chr8.1:29852758-29852777 None:intergenic 35.0%
AATCATGATTGCACTTCTCC+AGG + chr8.1:29853105-29853124 MS.gene76368:intron 40.0%
ACATACCTCTTTGTAGACCA+GGG - chr8.1:29853002-29853021 None:intergenic 40.0%
AGGTCATAGAGACTTGATCT+TGG + chr8.1:29852673-29852692 MS.gene76368:CDS 40.0%
CACAGCAATATGTCAAGTAC+TGG - chr8.1:29853074-29853093 None:intergenic 40.0%
CTATAAAACTCGGCATCAGT+GGG + chr8.1:29853246-29853265 MS.gene76368:CDS 40.0%
GAAACCGAGTCTGCATAAAA+AGG - chr8.1:29852907-29852926 None:intergenic 40.0%
GCAACCTCAACCAAAAAAGA+AGG + chr8.1:29852751-29852770 MS.gene76368:CDS 40.0%
GGTCATAGAGACTTGATCTT+GGG + chr8.1:29852674-29852693 MS.gene76368:CDS 40.0%
TAAACCCTTCAAGAAGATCC+GGG - chr8.1:29853153-29853172 None:intergenic 40.0%
TATAAAACTCGGCATCAGTG+GGG + chr8.1:29853247-29853266 MS.gene76368:CDS 40.0%
TTGTATGAGTGCACTGCAAA+GGG - chr8.1:29853307-29853326 None:intergenic 40.0%
! AGGTATGTGCTGTTATAGTC+AGG + chr8.1:29853014-29853033 MS.gene76368:intron 40.0%
! GGTTCCTTTTTATGCAGACT+CGG + chr8.1:29852900-29852919 MS.gene76368:intron 40.0%
! GTGTTGCGTAATTTTCGTGT+TGG + chr8.1:29853218-29853237 MS.gene76368:CDS 40.0%
! TAGGGCACCTCATAAAGTAA+TGG - chr8.1:29853385-29853404 None:intergenic 40.0%
! TTCATCTGTGTCGTGTTGAT+TGG + chr8.1:29853173-29853192 MS.gene76368:CDS 40.0%
! TTTATGCAGACTCGGTTTCA+GGG + chr8.1:29852908-29852927 MS.gene76368:intron 40.0%
!! AGCAATATGTCAAGTACTGG+AGG - chr8.1:29853071-29853090 None:intergenic 40.0%
!! TGGTGTCATTGAAAGAGTGA+AGG + chr8.1:29852640-29852659 MS.gene76368:CDS 40.0%
!! TTTTATGCAGACTCGGTTTC+AGG + chr8.1:29852907-29852926 MS.gene76368:intron 40.0%
AGGTACTTTCCCTCTTGGTA+GGG - chr8.1:29853403-29853422 None:intergenic 45.0%
CACATACCTCTTTGTAGACC+AGG - chr8.1:29853003-29853022 None:intergenic 45.0%
CTTGTATGAGTGCACTGCAA+AGG - chr8.1:29853308-29853327 None:intergenic 45.0%
GCTATAAAACTCGGCATCAG+TGG + chr8.1:29853245-29853264 MS.gene76368:CDS 45.0%
TATCACCCTGGTCTACAAAG+AGG + chr8.1:29852994-29853013 MS.gene76368:intron 45.0%
TCATTGACACCATTGCTCGA+AGG - chr8.1:29853341-29853360 None:intergenic 45.0%
TCTTTGTAGACCAGGGTGAT+AGG - chr8.1:29852995-29853014 None:intergenic 45.0%
TGCATTCTTCCAAAACCACC+CGG + chr8.1:29853132-29853151 MS.gene76368:CDS 45.0%
TGTATGAGTGCACTGCAAAG+GGG - chr8.1:29853306-29853325 None:intergenic 45.0%
TTTGGAAGAATGCAGCAACC+TGG - chr8.1:29853126-29853145 None:intergenic 45.0%
!! ATCTGTGTCGTGTTGATTGG+CGG + chr8.1:29853176-29853195 MS.gene76368:CDS 45.0%
AACCACCCGGATCTTCTTGA+AGG + chr8.1:29853145-29853164 MS.gene76368:CDS 50.0%
ACCACCCGGATCTTCTTGAA+GGG + chr8.1:29853146-29853165 MS.gene76368:CDS 50.0%
AGGGTAATCTCATGGCCCTT+TGG - chr8.1:29852716-29852735 None:intergenic 50.0%
CAGGTACTTTCCCTCTTGGT+AGG - chr8.1:29853404-29853423 None:intergenic 50.0%
CGGACGAAAGGGTAATCTCA+TGG - chr8.1:29852724-29852743 None:intergenic 50.0%
CTCATGGCCCTTTGGCAAAA+AGG - chr8.1:29852708-29852727 None:intergenic 50.0%
GGAAAGTAAGCCTATCACCC+TGG + chr8.1:29852982-29853001 MS.gene76368:intron 50.0%
TGAGATTACCCTTTCGTCCG+AGG + chr8.1:29852724-29852743 MS.gene76368:CDS 50.0%
TGGTTGAGGTTGCTCATCCT+CGG - chr8.1:29852744-29852763 None:intergenic 50.0%
TTGCTCATCCTCGGACGAAA+GGG - chr8.1:29852735-29852754 None:intergenic 50.0%
! ACTGCAAAGGGGTATGTCTC+AGG - chr8.1:29853295-29853314 None:intergenic 50.0%
! CAAGAAGATCCGGGTGGTTT+TGG - chr8.1:29853144-29853163 None:intergenic 50.0%
! TATGAGGTGCCCTACCAAGA+GGG + chr8.1:29853391-29853410 MS.gene76368:CDS 50.0%
! TTATGAGGTGCCCTACCAAG+AGG + chr8.1:29853390-29853409 MS.gene76368:CDS 50.0%
AAACTCGGCATCAGTGGGGA+AGG + chr8.1:29853251-29853270 MS.gene76368:CDS 55.0%
AACTCGGCATCAGTGGGGAA+GGG + chr8.1:29853252-29853271 MS.gene76368:CDS 55.0%
ACCCTTCAAGAAGATCCGGG+TGG - chr8.1:29853150-29853169 None:intergenic 55.0%
GTTGCTCATCCTCGGACGAA+AGG - chr8.1:29852736-29852755 None:intergenic 55.0%
!! TCGTGTTGATTGGCGGTGAC+TGG + chr8.1:29853183-29853202 MS.gene76368:CDS 55.0%
ACTCGGCATCAGTGGGGAAG+GGG + chr8.1:29853253-29853272 MS.gene76368:CDS 60.0%
CTCGGCATCAGTGGGGAAGG+GGG + chr8.1:29853254-29853273 MS.gene76368:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 29852533 29853424 29852533 ID=MS.gene76368
chr8.1 mRNA 29852533 29853424 29852533 ID=MS.gene76368.t1;Parent=MS.gene76368
chr8.1 exon 29852533 29852537 29852533 ID=MS.gene76368.t1.exon1;Parent=MS.gene76368.t1
chr8.1 CDS 29852533 29852537 29852533 ID=cds.MS.gene76368.t1;Parent=MS.gene76368.t1
chr8.1 exon 29852628 29852814 29852628 ID=MS.gene76368.t1.exon2;Parent=MS.gene76368.t1
chr8.1 CDS 29852628 29852814 29852628 ID=cds.MS.gene76368.t1;Parent=MS.gene76368.t1
chr8.1 exon 29852917 29852972 29852917 ID=MS.gene76368.t1.exon3;Parent=MS.gene76368.t1
chr8.1 CDS 29852917 29852972 29852917 ID=cds.MS.gene76368.t1;Parent=MS.gene76368.t1
chr8.1 exon 29853127 29853424 29853127 ID=MS.gene76368.t1.exon4;Parent=MS.gene76368.t1
chr8.1 CDS 29853127 29853424 29853127 ID=cds.MS.gene76368.t1;Parent=MS.gene76368.t1
Gene Sequence

>MS.gene76368

ATGTTGTACCACGTTGTTTTTGTATATATATTGGGAGAGGGATTATGAATTTGTTAGTTGTAATTGATTTGTTTATCCTTTTGGTGCATTTGTAGAGTTGATACTGATGGTGTCATTGAAAGAGTGAAGGAACTTTTTAGAGGTCATAGAGACTTGATCTTGGGATTTAACACCTTTTTGCCAAAGGGCCATGAGATTACCCTTTCGTCCGAGGATGAGCAACCTCAACCAAAAAAGAAGGTTGAATTTTATGAAGCTACGAGTTATGTGAACACGATAAAGGTTAGAATGTTTGTGAGTTTAATGGATTCTCTTCATGCAGATTTGTTTGCAATGTAGAATTAATAGTTTTAGTTTTGAGCTTAATGGTTCCTTTTTATGCAGACTCGGTTTCAGGGCGATGTTCATGTTTACAAGTCATTTATGGACATATTGAATACGTACAGAAAGGAAAGTAAGCCTATCACCCTGGTCTACAAAGAGGTATGTGCTGTTATAGTCAGGAAAATCTGTAATGTTGGTTTTTTTTTTATTTCCTCCAGTACTTGACATATTGCTGTGATACACATTTTAATCATGATTGCACTTCTCCAGGTTGCTGCATTCTTCCAAAACCACCCGGATCTTCTTGAAGGGTTTATTCATCTGTGTCGTGTTGATTGGCGGTGACTGGAAAGCTATCTGTGTGTTGCGTAATTTTCGTGTTGGTCATGCTATAAAACTCGGCATCAGTGGGGAAGGGGGTGCTGATGTTGTGTACCTGAGACATACCCCTTTGCAGTGCACTCATACAAGCTATATTCATCCTTCGAGCAATGGTGTCAATGAGAAATTTGTATATCAAGTTAACCATTACTTTATGAGGTGCCCTACCAAGAGGGAAAGTACCTGA

Protein sequence

>MS.gene76368.t1

MLVDTDGVIERVKELFRGHRDLILGFNTFLPKGHEITLSSEDEQPQPKKKVEFYEATSYVNTIKTRFQGDVHVYKSFMDILNTLLHSSKTTRIFLKGLFICVVLIGGDWKAICVLRNFRVGHAIKLGISGEGGADVVYLRHTPLQCTHTSYIHPSSNGVNEKFVYQVNHYFMRCPTKREST