Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76480.t1 | AET03910.1 | 97.8 | 185 | 4 | 0 | 1 | 185 | 68 | 252 | 3.20E-65 | 258.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76480.t1 | G7LDZ4 | 97.8 | 185 | 4 | 0 | 1 | 185 | 68 | 252 | 2.3e-65 | 258.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055453 | MS.gene76480 | 0.822032 | 2.94E-53 | -1.69E-46 |
| MS.gene055455 | MS.gene76480 | 0.822669 | 2.09E-53 | -1.69E-46 |
| MS.gene057156 | MS.gene76480 | 0.816686 | 4.87E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76480.t1 | MTR_8g077440 | 97.838 | 185 | 4 | 0 | 1 | 185 | 68 | 252 | 1.68e-130 | 366 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76480.t1 | AT3G51010 | 54.450 | 191 | 70 | 4 | 8 | 185 | 1 | 187 | 1.67e-61 | 189 |
Find 0 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATAAAATGAAATTAAG+TGG | + | 57545:10475-10494 | MS.gene76480:intergenic | 10.0% |
| !!! | TTAATTTCATTTTATATATC+AGG | - | 57545:10476-10495 | MS.gene76480:intron | 10.0% |
| !!! | TTCATTTTTTTAAAAAGAAT+AGG | + | 57545:11402-11421 | MS.gene76480:intergenic | 10.0% |
| !! | AATATAATTGTATGTTCATT+GGG | - | 57545:11533-11552 | MS.gene76480:CDS | 15.0% |
| !! | AATGAACATACAATTATATT+AGG | + | 57545:11533-11552 | MS.gene76480:intergenic | 15.0% |
| !! | TAATATAATTGTATGTTCAT+TGG | - | 57545:11532-11551 | MS.gene76480:CDS | 15.0% |
| !! | TATTTAACATAAACTAATCT+TGG | - | 57545:10754-10773 | MS.gene76480:intron | 15.0% |
| !! | AAAAAATGAAAGTTTCTCTA+TGG | - | 57545:11412-11431 | MS.gene76480:intron | 20.0% |
| !! | AAAGTTCAAATTCTTCTATA+AGG | + | 57545:11453-11472 | MS.gene76480:intergenic | 20.0% |
| !! | TATGAGTTTCATTTAAATCT+TGG | + | 57545:11023-11042 | MS.gene76480:intergenic | 20.0% |
| !! | TTATAGAAGAATTTGAACTT+TGG | - | 57545:11452-11471 | MS.gene76480:intron | 20.0% |
| !!! | AATTCTTCTATAAGGAATTT+TGG | + | 57545:11445-11464 | MS.gene76480:intergenic | 20.0% |
| !!! | AATTTTCTATTGAATGTCAA+CGG | - | 57545:11057-11076 | MS.gene76480:intron | 20.0% |
| !!! | ACTTTCTTATTTCTATTTTG+TGG | + | 57545:11114-11133 | MS.gene76480:intergenic | 20.0% |
| !!! | TTTCTATTTTGTGGTTATTA+TGG | + | 57545:11105-11124 | MS.gene76480:intergenic | 20.0% |
| ! | ATACTTATGAACGATAGATA+AGG | - | 57545:10872-10891 | MS.gene76480:intron | 25.0% |
| ! | CTTTGTGAATATTGTTTACT+AGG | - | 57545:10579-10598 | MS.gene76480:intron | 25.0% |
| ! | GATACCTTAAACATACATAT+AGG | + | 57545:11155-11174 | MS.gene76480:intergenic | 25.0% |
| ! | GCTAATATATACCAGTATAA+AGG | + | 57545:10453-10472 | MS.gene76480:intergenic | 25.0% |
| ! | TTGACCTATATGTATGTTTA+AGG | - | 57545:11148-11167 | MS.gene76480:intron | 25.0% |
| !! | AATAGGACATGTATTTTGTT+AGG | + | 57545:11385-11404 | MS.gene76480:intergenic | 25.0% |
| !! | GTTATTCTAGCTATGTATTA+GGG | - | 57545:11334-11353 | MS.gene76480:CDS | 25.0% |
| !! | TGTTATTCTAGCTATGTATT+AGG | - | 57545:11333-11352 | MS.gene76480:CDS | 25.0% |
| !!! | CTTTTATTTTTGCTACAAGT+TGG | + | 57545:10849-10868 | MS.gene76480:intergenic | 25.0% |
| ATAACAGCAAAGAAAACATG+AGG | + | 57545:10821-10840 | MS.gene76480:intergenic | 30.0% | |
| ATGTTTATCCAAAGACCATT+TGG | + | 57545:11489-11508 | MS.gene76480:intergenic | 30.0% | |
| GATAAACATAACTGTGATCA+TGG | - | 57545:11500-11519 | MS.gene76480:CDS | 30.0% | |
| GGTTATTATGGCTATAAGTA+TGG | + | 57545:11093-11112 | MS.gene76480:intergenic | 30.0% | |
| TATATACCAGTATAAAGGCA+CGG | + | 57545:10448-10467 | MS.gene76480:intergenic | 30.0% | |
| TGCATGGAGTATAAACTATA+AGG | - | 57545:10615-10634 | MS.gene76480:intron | 30.0% | |
| AAAAAGGGAAGAAGGAATTG+GGG | + | 57545:10146-10165 | MS.gene76479:intergenic | 35.0% | |
| AAATCTTGGACCATCTGTAT+TGG | + | 57545:11009-11028 | MS.gene76479:intergenic | 35.0% | |
| AACTATAAGGTGCATGAGTT+TGG | - | 57545:10628-10647 | MS.gene76480:intron | 35.0% | |
| AAGTGTCAACTAAAGCTAGA+AGG | - | 57545:11235-11254 | MS.gene76480:intron | 35.0% | |
| AATCTTGGACCATCTGTATT+GGG | + | 57545:11008-11027 | MS.gene76480:intergenic | 35.0% | |
| AGAAAAATGAGATTCGGAAG+AGG | - | 57545:9996-10015 | MS.gene76480:CDS | 35.0% | |
| AGGAAACATATCATCTTGCA+TGG | - | 57545:10599-10618 | MS.gene76480:intron | 35.0% | |
| ATAAGAAAGTGACAACTGAG+AGG | - | 57545:11124-11143 | MS.gene76480:intron | 35.0% | |
| ATTAGGCTTATTGGGAAGTA+TGG | + | 57545:10378-10397 | MS.gene76480:intergenic | 35.0% | |
| CAAAAAGGGAAGAAGGAATT+GGG | + | 57545:10147-10166 | MS.gene76480:intergenic | 35.0% | |
| CATACTATCATACCAGTATG+TGG | - | 57545:11174-11193 | MS.gene76480:intron | 35.0% | |
| GACTCACACATGTAAATCAA+AGG | + | 57545:10531-10550 | MS.gene76480:intergenic | 35.0% | |
| GGAAGAGAAGAAAAAGTCTA+TGG | - | 57545:11710-11729 | MS.gene76480:CDS | 35.0% | |
| GGTTGCAAAGAAGAATGAAA+CGG | + | 57545:10104-10123 | MS.gene76480:intergenic | 35.0% | |
| TAAGAAGGTAGAGCGTAAAA+TGG | - | 57545:11629-11648 | MS.gene76480:CDS | 35.0% | |
| TATATCAGCCAAATGGTCTT+TGG | - | 57545:11478-11497 | MS.gene76480:CDS | 35.0% | |
| TCAAAAAGGGAAGAAGGAAT+TGG | + | 57545:10148-10167 | MS.gene76480:intergenic | 35.0% | |
| TCTGTAACCATCATAAACAC+TGG | - | 57545:10951-10970 | MS.gene76480:intron | 35.0% | |
| TGCACGAGAAAAATGAGATT+CGG | - | 57545:9990-10009 | MS.gene76480:CDS | 35.0% | |
| TTCCCAATAAGCCTAATCAA+GGG | - | 57545:10381-10400 | MS.gene76480:intron | 35.0% | |
| TTGGTTGTATATCAGCCAAA+TGG | - | 57545:11471-11490 | MS.gene76480:CDS | 35.0% | |
| ! | ATTCTAGCTATGTATTAGGG+AGG | - | 57545:11337-11356 | MS.gene76480:CDS | 35.0% |
| !! | ACTTTTTCTTCTCTTCCTCA+AGG | + | 57545:11707-11726 | MS.gene76480:intergenic | 35.0% |
| !! | AGAAGTACCAGTGTTTATGA+TGG | + | 57545:10961-10980 | MS.gene76480:intergenic | 35.0% |
| !! | TTTGGAACAAAGGTATTGTC+TGG | - | 57545:10646-10665 | MS.gene76480:intron | 35.0% |
| !!! | TTGGGAACTTTTTTGCATGT+AGG | - | 57545:11551-11570 | MS.gene76480:CDS | 35.0% |
| ACAGCCAATTATTTGCCACA+GGG | + | 57545:11214-11233 | MS.gene76480:intergenic | 40.0% | |
| ACTCCATTACCCAATACAGA+TGG | - | 57545:10996-11015 | MS.gene76480:intron | 40.0% | |
| AGAAGAATGAAACGGCAATG+TGG | + | 57545:10096-10115 | MS.gene76480:intergenic | 40.0% | |
| CTCCCTTGATTAGGCTTATT+GGG | + | 57545:10386-10405 | MS.gene76480:intergenic | 40.0% | |
| CTGTGAAAGTGGCTCAAAAA+GGG | + | 57545:10161-10180 | MS.gene76480:intergenic | 40.0% | |
| CTTCCCAATAAGCCTAATCA+AGG | - | 57545:10380-10399 | MS.gene76480:intron | 40.0% | |
| TAAAATGGCTGCAGTTGCAA+GGG | - | 57545:11644-11663 | MS.gene76480:CDS | 40.0% | |
| TATGATGAACTTGTCGTCCA+GGG | - | 57545:10318-10337 | MS.gene76480:intron | 40.0% | |
| TCTTCTTTGCAACCATCTTC+CGG | - | 57545:10110-10129 | MS.gene76480:CDS | 40.0% | |
| TGTAGGCGGAAAAGAAGAAA+AGG | - | 57545:11568-11587 | MS.gene76480:CDS | 40.0% | |
| TTATCAGAAGGTCGTCTCTT+GGG | + | 57545:10203-10222 | MS.gene76480:intergenic | 40.0% | |
| TTATGATGAACTTGTCGTCC+AGG | - | 57545:10317-10336 | MS.gene76480:intron | 40.0% | |
| TTTATCAGAAGGTCGTCTCT+TGG | + | 57545:10204-10223 | MS.gene76480:intergenic | 40.0% | |
| !! | AACAAAGGTATTGTCTGGAG+TGG | - | 57545:10651-10670 | MS.gene76480:intron | 40.0% |
| !! | CACAGTGAAAGCATCTTTAG+AGG | - | 57545:10908-10927 | MS.gene76480:intron | 40.0% |
| !! | CTGTATTGGGTAATGGAGTT+AGG | + | 57545:10995-11014 | MS.gene76480:intergenic | 40.0% |
| !! | GGGTAATGGAGTTAGGTTTA+TGG | + | 57545:10988-11007 | MS.gene76480:intergenic | 40.0% |
| !! | GTTATGAGAGAGCATCAAGA+TGG | + | 57545:10780-10799 | MS.gene76480:intergenic | 40.0% |
| !!! | GGAACTTTTTTGCATGTAGG+CGG | - | 57545:11554-11573 | MS.gene76480:CDS | 40.0% |
| AAGGAAAGCTCAGATGCAAG+AGG | - | 57545:11587-11606 | MS.gene76480:CDS | 45.0% | |
| AGAATGAAACGGCAATGTGG+AGG | + | 57545:10093-10112 | MS.gene76480:intergenic | 45.0% | |
| AGCAGCAGCTGACAGATAAA+TGG | + | 57545:11313-11332 | MS.gene76480:intergenic | 45.0% | |
| AGCCAATTATTTGCCACAGG+GGG | + | 57545:11212-11231 | MS.gene76480:intergenic | 45.0% | |
| AGTGGCTCAAAAAGGGAAGA+AGG | + | 57545:10154-10173 | MS.gene76480:intergenic | 45.0% | |
| AGTTGCAAGGGAAAGAGAGT+GGG | - | 57545:11656-11675 | MS.gene76480:CDS | 45.0% | |
| CAGCCAATTATTTGCCACAG+GGG | + | 57545:11213-11232 | MS.gene76480:intergenic | 45.0% | |
| CAGTAATACGGACCACATAC+TGG | + | 57545:11189-11208 | MS.gene76480:intergenic | 45.0% | |
| CTCTTGATGCTCCCTTGATT+AGG | + | 57545:10395-10414 | MS.gene76480:intergenic | 45.0% | |
| GACAGCCAATTATTTGCCAC+AGG | + | 57545:11215-11234 | MS.gene76480:intergenic | 45.0% | |
| GCGCTGAGGCTAAATTGATT+TGG | - | 57545:11281-11300 | MS.gene76480:CDS | 45.0% | |
| GCGGAAGAAGAACGTTAAGA+AGG | - | 57545:11614-11633 | MS.gene76480:CDS | 45.0% | |
| GCTCCCTTGATTAGGCTTAT+TGG | + | 57545:10387-10406 | MS.gene76480:intergenic | 45.0% | |
| GCTGTGAAAGTGGCTCAAAA+AGG | + | 57545:10162-10181 | MS.gene76480:intergenic | 45.0% | |
| GGAATGAGAAGAAACGCATG+AGG | - | 57545:10417-10436 | MS.gene76480:intron | 45.0% | |
| GGACCATCTGTATTGGGTAA+TGG | + | 57545:11002-11021 | MS.gene76480:intergenic | 45.0% | |
| GTAAAATGGCTGCAGTTGCA+AGG | - | 57545:11643-11662 | MS.gene76480:CDS | 45.0% | |
| GTGTGTTCAACCCAAGCATA+AGG | + | 57545:10344-10363 | MS.gene76480:intergenic | 45.0% | |
| TACAACTTCACAATGCGCTG+AGG | - | 57545:11267-11286 | MS.gene76480:CDS | 45.0% | |
| TGTCTGTCAAGCTGTGAAAG+TGG | + | 57545:10172-10191 | MS.gene76480:intergenic | 45.0% | |
| TTTCGACGGGGTTTATCAGA+AGG | + | 57545:10215-10234 | MS.gene76480:intergenic | 45.0% | |
| ! | GGTGCATGAGTTTGGAACAA+AGG | - | 57545:10636-10655 | MS.gene76480:intron | 45.0% |
| !! | CTAATCAAGGGAGCATCAAG+AGG | - | 57545:10393-10412 | MS.gene76480:intron | 45.0% |
| AAGAGAGTGGGCAGAAAGAC+TGG | - | 57545:11668-11687 | MS.gene76480:CDS | 50.0% | |
| AAGGGAAGAAGGAATTGGGG+TGG | + | 57545:10143-10162 | MS.gene76480:intergenic | 50.0% | |
| ACGACGAGAGTGAGATCAGA+GGG | + | 57545:10043-10062 | MS.gene76480:intergenic | 50.0% | |
| AGATTCGGAAGAGGAGTCCT+CGG | - | 57545:10005-10024 | MS.gene76480:CDS | 50.0% | |
| CAGTTGCAAGGGAAAGAGAG+TGG | - | 57545:11655-11674 | MS.gene76480:CDS | 50.0% | |
| CTCAGATGCAAGAGGCTAAG+CGG | - | 57545:11595-11614 | MS.gene76480:CDS | 50.0% | |
| CTGATAAACCCCGTCGAAAG+CGG | - | 57545:10216-10235 | MS.gene76480:intron | 50.0% | |
| TGATAAACCCCGTCGAAAGC+GGG | - | 57545:10217-10236 | MS.gene76480:intron | 50.0% | |
| TGATCTCACTCTCGTCGTCA+AGG | - | 57545:10045-10064 | MS.gene76480:CDS | 50.0% | |
| TGCCCCCTGTGGCAAATAAT+TGG | - | 57545:11207-11226 | MS.gene76480:intron | 50.0% | |
| !! | ATCAAGGGAGCATCAAGAGG+AGG | - | 57545:10396-10415 | MS.gene76480:intron | 50.0% |
| !! | GCTTGGGTTGAACACACAAC+TGG | - | 57545:10347-10366 | MS.gene76480:intron | 50.0% |
| AAGCAACGCAACGCACCAGA+GGG | + | 57545:10254-10273 | MS.gene76480:intergenic | 55.0% | |
| CTCACTCTCGTCGTCAAGGA+CGG | - | 57545:10049-10068 | MS.gene76480:CDS | 55.0% | |
| GACGACGAGAGTGAGATCAG+AGG | + | 57545:10044-10063 | MS.gene76480:intergenic | 55.0% | |
| GATTCGGAAGAGGAGTCCTC+GGG | - | 57545:10006-10025 | MS.gene76480:CDS | 55.0% | |
| GGTTGAATTGCAGCGCCTTG+AGG | - | 57545:11689-11708 | MS.gene76480:CDS | 55.0% | |
| TCAACCCAAGCATAAGGCCC+TGG | + | 57545:10338-10357 | MS.gene76480:intergenic | 55.0% | |
| TCAAGCTAGCCCGCTTTCGA+CGG | + | 57545:10229-10248 | MS.gene76480:intergenic | 55.0% | |
| TGCCACAGGGGGCAGTAATA+CGG | + | 57545:11201-11220 | MS.gene76480:intergenic | 55.0% | |
| ! | GAGGGCGTAAAACATTCCCG+AGG | + | 57545:10025-10044 | MS.gene76480:intergenic | 55.0% |
| ! | TCGTCCAGGGCCTTATGCTT+GGG | - | 57545:10331-10350 | MS.gene76480:intron | 55.0% |
| !! | GAATTGGGGTGGTTTGCTGC+CGG | + | 57545:10132-10151 | MS.gene76480:intergenic | 55.0% |
| AAGCTAGCCCGCTTTCGACG+GGG | + | 57545:10227-10246 | MS.gene76480:intergenic | 60.0% | |
| CAAGCTAGCCCGCTTTCGAC+GGG | + | 57545:10228-10247 | MS.gene76480:intergenic | 60.0% | |
| GAAGCAACGCAACGCACCAG+AGG | + | 57545:10255-10274 | MS.gene76480:intergenic | 60.0% | |
| GCAGCGCCGTGCCTTTATAC+TGG | - | 57545:10439-10458 | MS.gene76480:intron | 60.0% | |
| GTCCGTATTACTGCCCCCTG+TGG | - | 57545:11196-11215 | MS.gene76480:intron | 60.0% | |
| ! | GTCGTCCAGGGCCTTATGCT+TGG | - | 57545:10330-10349 | MS.gene76480:intron | 60.0% |
| !! | GGTGGTTTGCTGCCGGAAGA+TGG | + | 57545:10125-10144 | MS.gene76480:intergenic | 60.0% |
| !! | CGGGCTAGCTTGAGACCCTC+TGG | - | 57545:10236-10255 | MS.gene76480:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57545 | gene | 9987 | 11737 | 9987 | ID=MS.gene76480 |
| 57545 | mRNA | 9987 | 11737 | 9987 | ID=MS.gene76480.t1;Parent=MS.gene76480 |
| 57545 | exon | 11467 | 11737 | 11467 | ID=MS.gene76480.t1.exon1;Parent=MS.gene76480.t1 |
| 57545 | CDS | 11467 | 11737 | 11467 | ID=cds.MS.gene76480.t1;Parent=MS.gene76480.t1 |
| 57545 | exon | 11264 | 11385 | 11264 | ID=MS.gene76480.t1.exon2;Parent=MS.gene76480.t1 |
| 57545 | CDS | 11264 | 11385 | 11264 | ID=cds.MS.gene76480.t1;Parent=MS.gene76480.t1 |
| 57545 | exon | 9987 | 10151 | 9987 | ID=MS.gene76480.t1.exon3;Parent=MS.gene76480.t1 |
| 57545 | CDS | 9987 | 10151 | 9987 | ID=cds.MS.gene76480.t1;Parent=MS.gene76480.t1 |
| Gene Sequence |
| Protein sequence |
