AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055453


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055453.t1 MTR_3g109360 98.431 255 4 0 1 255 1 255 0.0 522
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055453.t1 AT5G24840 79.012 243 48 2 8 249 8 248 2.07e-144 404

Find 65 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATTAAGTTTGCCGATAT+TGG 0.221557 3.3:-89684825 MS.gene055453:CDS
TCCGCACCACAGAAACATTT+TGG 0.250656 3.3:+89684661 None:intergenic
ATTCCGATCATCAATGTTTC+TGG 0.252188 3.3:+89684759 None:intergenic
GCCGATATTGGCTGTGGTTT+TGG 0.274610 3.3:-89684813 MS.gene055453:CDS
CCTCGTTTCTAGCAACCTTT+TGG 0.326676 3.3:+89684331 None:intergenic
TAAAATGAGGATCGGGAAAC+AGG 0.368009 3.3:+89684577 None:intergenic
TTCTCTTTAAAATGAGGATC+GGG 0.398927 3.3:+89684570 None:intergenic
GCATACTCATCTAGTAAGAA+TGG 0.400409 3.3:+89684522 None:intergenic
AAACGAGGGACAAACTTTCC+AGG 0.404412 3.3:-89684316 MS.gene055453:CDS
TTGTGCTTTCTCAAAGTGAT+TGG 0.404689 3.3:+89684617 None:intergenic
GGACTGGATGAAATCTTGTT+TGG 0.409104 3.3:-89684442 MS.gene055453:CDS
TCCAAAACCACAGCCAATAT+CGG 0.417152 3.3:+89684812 None:intergenic
TTAAGCTCTGCCACTGAAGA+AGG 0.428496 3.3:-89684354 MS.gene055453:CDS
TGTGCTTTCTCAAAGTGATT+GGG 0.444654 3.3:+89684618 None:intergenic
TCAACATTCAGTAAGTCAAC+AGG 0.452366 3.3:-89684999 MS.gene055453:CDS
GTGATTGGGAATGTACTTCA+TGG 0.454613 3.3:+89684632 None:intergenic
GTTGAATTTCCACCAGTCTC+AGG 0.455044 3.3:+89685017 None:intergenic
TGGTTCTTCTCTTTAAAATG+AGG 0.455252 3.3:+89684564 None:intergenic
CAGTCAAAGGTTCAAACAAC+GGG 0.464877 3.3:+89684412 None:intergenic
TAAAGTGTCCGAGTATGTTA+AGG 0.471708 3.3:-89684724 MS.gene055453:CDS
TCAAGTTCTTTCTCAGTCAA+AGG 0.473439 3.3:+89684399 None:intergenic
ATAACAGATGTGGAAGAACT+TGG 0.476880 3.3:-89684465 MS.gene055453:CDS
GCTCGATAGAAGCGCTTTCG+AGG 0.477207 3.3:+89684972 None:intergenic
TCTTTAAGGGTAGCAAATCC+AGG 0.486768 3.3:-89684690 MS.gene055453:CDS
GAAAGAACTTGAAGCAGATC+CGG 0.487319 3.3:-89684388 MS.gene055453:CDS
TTCCGATCATCAATGTTTCT+GGG 0.488618 3.3:+89684760 None:intergenic
AGAGAAGAACCATCGCCGTC+GGG 0.492041 3.3:-89684553 MS.gene055453:CDS
GAGGGAATAGTCAACATGAC+TGG 0.493842 3.3:+89684896 None:intergenic
CTTCTCTTTAAAATGAGGAT+CGG 0.494124 3.3:+89684569 None:intergenic
CATACTCATCTAGTAAGAAT+GGG 0.503167 3.3:+89684523 None:intergenic
TGCCACTGAAGAAGGCCAAA+AGG 0.507812 3.3:-89684346 MS.gene055453:CDS
TCAACATGACTGGGTGATAA+CGG 0.513146 3.3:+89684906 None:intergenic
AGGGAAAGAACTGAGGGTAA+TGG 0.514591 3.3:+89684874 None:intergenic
AAGTTTGCCGATATTGGCTG+TGG 0.514722 3.3:-89684819 MS.gene055453:CDS
GAAAGAACTGAGGGTAATGG+AGG 0.517145 3.3:+89684877 None:intergenic
TAACAGATGTGGAAGAACTT+GGG 0.526686 3.3:-89684464 MS.gene055453:CDS
AAGAGAAGAACCATCGCCGT+CGG 0.534336 3.3:-89684554 MS.gene055453:CDS
CTATCAGCTTTCTCAGACGA+GGG 0.534942 3.3:+89684855 None:intergenic
CAATGCGTCTGTATACAGCC+TGG 0.550730 3.3:+89684298 None:intergenic
GGCTGATAACCCGACGGCGA+TGG 0.555787 3.3:+89684544 None:intergenic
TAAACAGACATGCCTGAGAC+TGG 0.565306 3.3:-89685029 None:intergenic
ACCAAAATGTTTCTGTGGTG+CGG 0.567556 3.3:-89684662 MS.gene055453:CDS
AATTCGTTCCTTAACATACT+CGG 0.570956 3.3:+89684716 None:intergenic
TTCCCAGAAACATTGATGAT+CGG 0.572410 3.3:-89684762 MS.gene055453:CDS
GAGCTTAAAAGCTTAACAGC+CGG 0.573038 3.3:+89684369 None:intergenic
ATGTGGAAGAACTTGGGGAC+TGG 0.574117 3.3:-89684458 MS.gene055453:CDS
TGACTGGGTGATAACGGCAC+AGG 0.576851 3.3:+89684912 None:intergenic
AGAAACATTGATGATCGGAA+TGG 0.582107 3.3:-89684757 MS.gene055453:CDS
AAAGAACTGAGGGTAATGGA+GGG 0.599329 3.3:+89684878 None:intergenic
AACAGATGTGGAAGAACTTG+GGG 0.605004 3.3:-89684463 MS.gene055453:CDS
GGAAATGAGAGTCACTGAGC+GGG 0.605858 3.3:+89684934 None:intergenic
GACTGGGTGATAACGGCACA+GGG 0.611699 3.3:+89684913 None:intergenic
TCAGTCAAAGGTTCAAACAA+CGG 0.617042 3.3:+89684411 None:intergenic
TCAATACCAAAATGTTTCTG+TGG 0.618646 3.3:-89684667 MS.gene055453:CDS
CCAAAAGGTTGCTAGAAACG+AGG 0.621682 3.3:-89684331 MS.gene055453:CDS
TTATAACACAATAACAGATG+TGG 0.624043 3.3:-89684475 MS.gene055453:CDS
AGGGAATAGTCAACATGACT+GGG 0.631622 3.3:+89684897 None:intergenic
ACAGACATGCCTGAGACTGG+TGG 0.638512 3.3:-89685026 None:intergenic
GGGAAATGAGAGTCACTGAG+CGG 0.640601 3.3:+89684933 None:intergenic
CAAAAGGTTGCTAGAAACGA+GGG 0.641444 3.3:-89684330 MS.gene055453:CDS
ACTATCAGCTTTCTCAGACG+AGG 0.648094 3.3:+89684854 None:intergenic
CAATGTTTCTGGGAACAGAG+TGG 0.655902 3.3:+89684770 None:intergenic
CAGACGAGGGAAAGAACTGA+GGG 0.659441 3.3:+89684868 None:intergenic
TCAGACGAGGGAAAGAACTG+AGG 0.695327 3.3:+89684867 None:intergenic
AGAATGGGCTGATAACCCGA+CGG 0.724299 3.3:+89684538 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTATAACACAATAACAGATG+TGG - chr3.3:89684820-89684839 MS.gene055453:CDS 25.0%
!! AGGAACGAATTTTATCTTTA+AGG - chr3.3:89684591-89684610 MS.gene055453:CDS 25.0%
!! GGAACGAATTTTATCTTTAA+GGG - chr3.3:89684592-89684611 MS.gene055453:CDS 25.0%
AAGATTAAGTTTGCCGATAT+TGG - chr3.3:89684470-89684489 MS.gene055453:CDS 30.0%
AATTCGTTCCTTAACATACT+CGG + chr3.3:89684582-89684601 None:intergenic 30.0%
CATACTCATCTAGTAAGAAT+GGG + chr3.3:89684775-89684794 None:intergenic 30.0%
! TATGCTTTTGTTCTTGAGAT+TGG - chr3.3:89684791-89684810 MS.gene055453:CDS 30.0%
! TCAATACCAAAATGTTTCTG+TGG - chr3.3:89684628-89684647 MS.gene055453:CDS 30.0%
!! CTTCTCTTTAAAATGAGGAT+CGG + chr3.3:89684729-89684748 None:intergenic 30.0%
!! TGGTTCTTCTCTTTAAAATG+AGG + chr3.3:89684734-89684753 None:intergenic 30.0%
!! TTCTCTTTAAAATGAGGATC+GGG + chr3.3:89684728-89684747 None:intergenic 30.0%
ATAACAGATGTGGAAGAACT+TGG - chr3.3:89684830-89684849 MS.gene055453:CDS 35.0%
ATTCCGATCATCAATGTTTC+TGG + chr3.3:89684539-89684558 None:intergenic 35.0%
GCATACTCATCTAGTAAGAA+TGG + chr3.3:89684776-89684795 None:intergenic 35.0%
TAACAGATGTGGAAGAACTT+GGG - chr3.3:89684831-89684850 MS.gene055453:CDS 35.0%
TCAACATTCAGTAAGTCAAC+AGG - chr3.3:89684296-89684315 MS.gene055453:CDS 35.0%
TCAAGTTCTTTCTCAGTCAA+AGG + chr3.3:89684899-89684918 None:intergenic 35.0%
TCAGTCAAAGGTTCAAACAA+CGG + chr3.3:89684887-89684906 None:intergenic 35.0%
TGTGCTTTCTCAAAGTGATT+GGG + chr3.3:89684680-89684699 None:intergenic 35.0%
TTCCGATCATCAATGTTTCT+GGG + chr3.3:89684538-89684557 None:intergenic 35.0%
TTGTGCTTTCTCAAAGTGAT+TGG + chr3.3:89684681-89684700 None:intergenic 35.0%
! AGAAACATTGATGATCGGAA+TGG - chr3.3:89684538-89684557 MS.gene055453:CDS 35.0%
! TAAAGTGTCCGAGTATGTTA+AGG - chr3.3:89684571-89684590 MS.gene055453:CDS 35.0%
! TTCCCAGAAACATTGATGAT+CGG - chr3.3:89684533-89684552 MS.gene055453:CDS 35.0%
!!! GAAACATTTTGGTATTGACC+TGG + chr3.3:89684626-89684645 None:intergenic 35.0%
AACAGATGTGGAAGAACTTG+GGG - chr3.3:89684832-89684851 MS.gene055453:CDS 40.0%
AGGGAATAGTCAACATGACT+GGG + chr3.3:89684401-89684420 None:intergenic 40.0%
CAAAAGGTTGCTAGAAACGA+GGG - chr3.3:89684965-89684984 MS.gene055453:CDS 40.0%
CAGTCAAAGGTTCAAACAAC+GGG + chr3.3:89684886-89684905 None:intergenic 40.0%
GAAAGAACTTGAAGCAGATC+CGG - chr3.3:89684907-89684926 MS.gene055453:CDS 40.0%
GAGCTTAAAAGCTTAACAGC+CGG + chr3.3:89684929-89684948 None:intergenic 40.0%
GGACTGGATGAAATCTTGTT+TGG - chr3.3:89684853-89684872 MS.gene055453:CDS 40.0%
GTGATTGGGAATGTACTTCA+TGG + chr3.3:89684666-89684685 None:intergenic 40.0%
TAAAATGAGGATCGGGAAAC+AGG + chr3.3:89684721-89684740 None:intergenic 40.0%
TCAACATGACTGGGTGATAA+CGG + chr3.3:89684392-89684411 None:intergenic 40.0%
TCCAAAACCACAGCCAATAT+CGG + chr3.3:89684486-89684505 None:intergenic 40.0%
! ACCAAAATGTTTCTGTGGTG+CGG - chr3.3:89684633-89684652 MS.gene055453:CDS 40.0%
! GCTTTTGTTCTTGAGATTGG+TGG - chr3.3:89684794-89684813 MS.gene055453:CDS 40.0%
! TCTTTAAGGGTAGCAAATCC+AGG - chr3.3:89684605-89684624 MS.gene055453:CDS 40.0%
!! AAAGAACTGAGGGTAATGGA+GGG + chr3.3:89684420-89684439 None:intergenic 40.0%
!!! ATATTGGCTGTGGTTTTGGA+GGG - chr3.3:89684486-89684505 MS.gene055453:CDS 40.0%
AAACGAGGGACAAACTTTCC+AGG - chr3.3:89684979-89684998 MS.gene055453:CDS 45.0%
ACTATCAGCTTTCTCAGACG+AGG + chr3.3:89684444-89684463 None:intergenic 45.0%
AGGGAAAGAACTGAGGGTAA+TGG + chr3.3:89684424-89684443 None:intergenic 45.0%
CCAAAAGGTTGCTAGAAACG+AGG - chr3.3:89684964-89684983 MS.gene055453:CDS 45.0%
CTATCAGCTTTCTCAGACGA+GGG + chr3.3:89684443-89684462 None:intergenic 45.0%
GAGGGAATAGTCAACATGAC+TGG + chr3.3:89684402-89684421 None:intergenic 45.0%
GTTGAATTTCCACCAGTCTC+AGG + chr3.3:89684281-89684300 None:intergenic 45.0%
TTAAGCTCTGCCACTGAAGA+AGG - chr3.3:89684941-89684960 MS.gene055453:CDS 45.0%
! AAGTTTGCCGATATTGGCTG+TGG - chr3.3:89684476-89684495 MS.gene055453:CDS 45.0%
! GAAAGAACTGAGGGTAATGG+AGG + chr3.3:89684421-89684440 None:intergenic 45.0%
! TCCGCACCACAGAAACATTT+TGG + chr3.3:89684637-89684656 None:intergenic 45.0%
!! AACCTTTTGGCCTTCTTCAG+TGG + chr3.3:89684954-89684973 None:intergenic 45.0%
!! CAATGTTTCTGGGAACAGAG+TGG + chr3.3:89684528-89684547 None:intergenic 45.0%
!!! CCTCGTTTCTAGCAACCTTT+TGG + chr3.3:89684967-89684986 None:intergenic 45.0%
!!! GATATTGGCTGTGGTTTTGG+AGG - chr3.3:89684485-89684504 MS.gene055453:CDS 45.0%
AAGAGAAGAACCATCGCCGT+CGG - chr3.3:89684741-89684760 MS.gene055453:CDS 50.0%
ATGTGGAAGAACTTGGGGAC+TGG - chr3.3:89684837-89684856 MS.gene055453:CDS 50.0%
CAATGCGTCTGTATACAGCC+TGG + chr3.3:89685000-89685019 None:intergenic 50.0%
CAGACGAGGGAAAGAACTGA+GGG + chr3.3:89684430-89684449 None:intergenic 50.0%
GGAAATGAGAGTCACTGAGC+GGG + chr3.3:89684364-89684383 None:intergenic 50.0%
GGGAAATGAGAGTCACTGAG+CGG + chr3.3:89684365-89684384 None:intergenic 50.0%
TCAGACGAGGGAAAGAACTG+AGG + chr3.3:89684431-89684450 None:intergenic 50.0%
TGCCACTGAAGAAGGCCAAA+AGG - chr3.3:89684949-89684968 MS.gene055453:CDS 50.0%
!! AGAATGGGCTGATAACCCGA+CGG + chr3.3:89684760-89684779 None:intergenic 50.0%
!! GCCGATATTGGCTGTGGTTT+TGG - chr3.3:89684482-89684501 MS.gene055453:CDS 50.0%
AGAGAAGAACCATCGCCGTC+GGG - chr3.3:89684742-89684761 MS.gene055453:CDS 55.0%
!! GACTGGGTGATAACGGCACA+GGG + chr3.3:89684385-89684404 None:intergenic 55.0%
!! GCTCGATAGAAGCGCTTTCG+AGG + chr3.3:89684326-89684345 None:intergenic 55.0%
!! TGACTGGGTGATAACGGCAC+AGG + chr3.3:89684386-89684405 None:intergenic 55.0%
GGCTGATAACCCGACGGCGA+TGG + chr3.3:89684754-89684773 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 89684275 89685042 89684275 ID=MS.gene055453
chr3.3 mRNA 89684275 89685042 89684275 ID=MS.gene055453.t1;Parent=MS.gene055453
chr3.3 exon 89684275 89685042 89684275 ID=MS.gene055453.t1.exon1;Parent=MS.gene055453.t1
chr3.3 CDS 89684275 89685042 89684275 ID=cds.MS.gene055453.t1;Parent=MS.gene055453.t1
Gene Sequence

>MS.gene055453

ATGCCTGAGACTGGTGGAAATTCAACATTCAGTAAGTCAACAGGATTGCCTCGAAAGCGCTTCTATCGAGCACGAGCACACAGCAACCCGCTCAGTGACTCTCATTTCCCTGTGCCGTTATCACCCAGTCATGTTGACTATTCCCTCCATTACCCTCAGTTCTTTCCCTCGTCTGAGAAAGCTGATAGTTGCAAAAAGATTAAGTTTGCCGATATTGGCTGTGGTTTTGGAGGGTTGCTCATCAGTCTTTCCACTCTGTTCCCAGAAACATTGATGATCGGAATGGAACTTAGAGATAAAGTGTCCGAGTATGTTAAGGAACGAATTTTATCTTTAAGGGTAGCAAATCCAGGTCAATACCAAAATGTTTCTGTGGTGCGGACTAACTCCATGAAGTACATTCCCAATCACTTTGAGAAAGCACAACTCTCAAAGATGTTTTTCCTGTTTCCCGATCCTCATTTTAAAGAGAAGAACCATCGCCGTCGGGTTATCAGCCCATTCTTACTAGATGAGTATGCTTTTGTTCTTGAGATTGGTGGAATTTATAACACAATAACAGATGTGGAAGAACTTGGGGACTGGATGAAATCTTGTTTGGAGAATCACCCGTTGTTTGAACCTTTGACTGAGAAAGAACTTGAAGCAGATCCGGCTGTTAAGCTTTTAAGCTCTGCCACTGAAGAAGGCCAAAAGGTTGCTAGAAACGAGGGACAAACTTTCCAGGCTGTATACAGACGCATTGCAGCATCTGATCAAATATCCTAG

Protein sequence

>MS.gene055453.t1

MPETGGNSTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPLSPSHVDYSLHYPQFFPSSEKADSCKKIKFADIGCGFGGLLISLSTLFPETLMIGMELRDKVSEYVKERILSLRVANPGQYQNVSVVRTNSMKYIPNHFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAFVLEIGGIYNTITDVEELGDWMKSCLENHPLFEPLTEKELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIAASDQIS